Claims
- 1. A bioaffinity separation process comprising the steps of:
- (A) forming a liquid affinity support by attaching a protein having available amino groups to the surface of the droplets of an emulsion of a liquid perfluorocarbon carrier through a highly fluorinated isocyanate anchor group by placing said anchor groups on about 20% of the available amino groups of said protein; and
- (B) capturing a target ligand or binder for the the ligand, complementary to the protein attached to the carrier from a mixture using said liquid affinity support.
- 2. The process of claim 1 wherein said ligand is selected from the group consisting of enzyme substrate and enzyme inhibitors.
- 3. The process of claim 1 wherein said binder for the ligand is selected from the group consisting of antibody, enzyme, and binding protein.
- 4. The process of claim 1 wherein said ligand is an antigen.
- 5. The process of claim 1 wherein said ligand is a hapten.
CROSS REFERENCE TO RELATED APPLICATIONS
This is a division of application Ser. No. 07/134,026, filed Dec. 17, 1987, now abandoned which is a continuation-in-part of an application entitled Bioaffinity Separations with Liquid Affinity Supports, Ser. No. 032,642, filed Mar. 31, 1987 which is a continuation-in-part of Ser. No. 863,607, filed May 15, 1986 both by Julian P. Breillatt, Jr. et al.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
4101380 |
Rubinstein et al. |
Jul 1978 |
|
4619904 |
Giaever et al. |
Oct 1986 |
|
4634681 |
Giaever et al. |
Jan 1987 |
|
Divisions (1)
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Number |
Date |
Country |
Parent |
134026 |
Dec 1987 |
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Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
32642 |
Mar 1987 |
|
Parent |
863607 |
May 1986 |
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