Bioassay element and its producing method

Abstract

The present invention relates to a bioassay element and its producing method. The bioassay element includes a carrier, a testing material, and a coating film, in which the testing material is dispersed in the coating film which covers on the surface of the carrier and comprises a casein and calcium ions. The bioassay element is produced by applying a bioassay solution which includes a testing material, a casein and calcium ions to the carrier, and then drying the bioassay solution.

Description

BRIEF DESCRIPTION OF FIGURES

FIG. 1 illustrates a structure of the bioassay element of one embodiment of the present invention.

FIG. 2 is a flow chart illustrating a process for preparing the bioassay element of one embodiment of the present invention.

FIG. 3 shows enzyme activity in the bioassay elements of a prior art, a comparative example, and a working example of the present invention.

FIG. 4 shows enzyme activity of the bioassay element produced in the working example of the present invention.

FIG. 5 shows inhibition of the bioassay element produced in the working example of the present invention against several pesticides at different concentrations.

FIG. 6 shows color of the bioassay element produced in the working example of the present invention against several pesticides at different concentrations.

Claims

1. A bioassay element,comprising: a carrier;a coating film, which covers on the surface of said carrier and comprises a casein and calcium ions; anda testing material, which disperses in said coating membrane.

2. A bioassay element according to claim 1, wherein said testing material is an enzyme.

3. A bioassay element according to claim 2, wherein said enzyme is acetylcholinesterase.

4. A bioassay element according to claim 1, wherein said carrier includes a membrane and a stationary layer applying on said membrane, and said coating film covers on the surface of said stationary layer.

5. A bioassay element according to claim 4, wherein said stationary layer comprises a casein and calcium ions.

6. A bioassay element according to claim 1, wherein said coating film further comprises a color developer.

7. A bioassay element according to claim 1, wherein said coating film further comprises trehalose.

8. A method of producing a bioassay element, which comprises steps of: providing a carrier;applying a bioassay solution which includes a testing material, a casein and calcium ions to said carrier; anddrying said bioassay solution.

9. A method according to claim 8, wherein said testing material is an enzyme.

10. A method according to claim 9, wherein said enzyme is acetylcholinesterase.

11. A method according to claim 8, wherein the step of providing a carrier further includes the steps of: applying a mixed solution which includes a casein and calcium ions on a membrane; anddrying said mixed solution to form a stationary layer on said membrane.

12. A method according to claim 11, wherein said mixed solution is dried by means of air drying, vacuum drying, suction drying, or a combination thereof.

13. A method according to claim 11, wherein said mixed solution is prepared by mixing 120 mM of a calcium ion solution and 2.5% of a casein solution in a ratio of 1:14 by volume.

14. A method according to claim 8, wherein said bioassay solution is dried by means of air drying, vacuum drying, suction drying, or a combination thereof.

15. A method according to claim 8, wherein said bioassay solution is prepared by mixing an enzyme solution and a mixed solution of casein in a ratio of 5:7 by volume.

16. A method according to claim 15, wherein said enzyme solution is prepared by mixing 1.52 U/μl of an enzyme and 5% of trehalose in a ratio of 2.61:108 by volume.

17. A method according to claim 15, wherein said mixed solution of casein is prepared by mixing a color developer, 120 mM of a calcium ion solution and 4% of a casein solution in a ratio of (0.009 to 0.012):1:(6 to 7.75) (w/v/v).