Patent Grant
7514105
The present invention relates to a composition containing extract and/or bioactive fractions from the plant Cuminum cyminum as a bioavailability enhancer. The present invention also relates to a composition containing extract and/or bioactive fractions from the plant Cuminum cyminum with piperine as a bioavailability enhancer. The present invention in addition relates to the use of bioavailability and/or bioefficacy enhancers—also termed as bioenhancers or BE and methods of their preparation which include their isolation from a natural source and obtaining the final products in their chemically characterized or fingerprint-profiled form.
Several approaches have been adopted in the past to maximize oral bioavailability, such as (a) particle size reduction (micronization, nanonization, etc.,) (b) polymorphic or crystal size and form selection, (c) solubilization of lesser soluble drugs by way of chemical modifications, complexation and use of co-solvents/surfactants, (d) targeted delivery of drug at the site of action, (e) controlled drug delivery by film coating or use of polymeric matrices for sustained release of drugs, (f) prodrug approach, and (g) micro-encapsulation using liposomes.
However, based on clues from Ayurvedic literature, a new approach of increasing the bioavailability of drugs including poorly-bioavailable drugs had been conceptualized at applicants institute Regional Research Laboratory (RRL), Jammu. One of the groups of herbals which has been documented very frequently as essential part of about 70% of Ayurvedic prescriptions, was noted to be ‘Trikatu’, that comprises three acrids viz. long pepper, black pepper and dry ginger in equal proportions. A single major alkaloidal constituent from peppers (piperine) was found to be responsible for bioavailability enhancing effect. The role of ginger is to regulate intestinal function to facilitate absorption. Influence of piperine was extensively studied on anti-TB drugs. It was determined that in combination with piperine, the dose of rifampicin can be reduced by about 50% while retaining the therapeutic efficacy of this anti-TB drug at par with the standard dose (450 mg). Based on these findings several other reputed plants were evaluated for bioavailability/bioefficacy enhancing activity. Polar and non-polar extracts of parts of a few plants viz., Zingiber officinale, and Carum carvi increased significantly (25-300%), [Applicants co-pending patent applications] the bioavailability of a number of classes of drugs, for example, but not limited to, antibiotics, antifungals, anti-virals, anticancer, cardiovascular, CNS, anti-inflammatory/anti-arthritic, anti-TB/antileprosy, anti-histaminic/respiratory disorders, corticosteroids, immunosuppressants, anti-ulcer. Such extracts either in presence or absence of piperine have been found to be highly selective in their bioavailability/bioefficacy enhancing action.
Cuminum cyminum (Linn.) (Umbelliferae) is a small, slender annual herb, which is grown extensively in South-Eastern Europe, North Africa bordering the Mediterranean-sea, in India and China. It is cultivated in almost all the states in India. The chief areas are reported to be U.P., Punjab, Rajasthan, Gujarat and Maharashtra. The plant prefers a mild climate and grows from sea level up to an elevation of 10,000 feet.
Its seeds have been used as an important condiment. In Ayurveda it is documented as katu, ushna and pacifies deranged vata. It is an effective gastric stimulant, beneficial in abdominal lump, flatulence, diarrhoea, sprue and a strong anthelmintic. It has therapeutically been used as an anti-diarrhoeal, galactagogue, diuretic and also beneficial in hoarseness of voice. It is also used as astringent, carminative. Paste of seeds when applied externally allays pain and irritation due to worms in the abdomen. Oil is useful in eczema.
Based on our past experience with the development of piperine as bioavailability enhancer from plants which are otherwise part of human diet and also documented to possess medicinal properties, we took up this plant to evaluate its bioavailability enhancing effect, if any, based on its attributes bearing some similarities to the plant sources of piperine.
Chemistry of Cuminum cyminum
The seeds of Cuminum cyminum were analyzed and it was reported that, analytical contents of seeds are (in percentage): moisture 11.9; protein 18.7; ether extractive 15.0; carbohydrates 36.6; fibre 12.0; mineral matter 5.8; calcium 1.08; phosphorus 0.49%; iron 31.0 mg/100 g; carotene calculated as vitamin A 870 1.U./100 gm; and vitamin C 3.0 mg/100 g (Hlth Bull., No. 23, 1941,36).
The seeds on distillation yield a volatile oil (2.0-4.0%) having an unpleasant characteristic odour, spicy and somewhat bitter taste. The oil is colourless or yellow when fresh, turning dark on keeping. The analytical constants of the Indian oil are d15°, 0.8945; nD25° 1.490; [α]D25°, +3.60; aldehydes 16%; the oil is soluble in 11 volume of 80% alcohol at 20° C. The ranges of constants reported by Parry are: sp. gr. 0.900-0.930;
n=1.494−1.507; [α]+3.0 to +8.0°; aldehydes 25-35% (Rao et al, J. Indian Inst. Sci., 1925, 8A, 182; Parry, E. J. “The Chemistry of Essential Oils and Artificial Perfumes” [1921] (Scott, Greenwood & Son Ltd., London) Vol. 1, p.311).
The chief constituent of the volatile oil is cuminaldehyde (C10H12O, p-isopropylbenzaldehyde, b.p. 235°), which forms nearly 20-40% of the oil. Besides the aldehyde, oil contains p-cymene, pinene, dipentene, cumene, cuminic alcohol, β-phellandrene and α-terpineol. The residue left after the volatile oil extraction contains 17.2% protein and 30.0% fat. It can be used as cattle feed (Finnemore, H. “The Essential Oils” [1926] (Ernest Benn. Ltd., London) p. 641). Besides volatile oil seeds contains 10% fixed oil, which is greenish brown in color with a strong aromatic flavor.
Other chemical constituents reported are apigenin-7-glucoside, apigenin-7-diglucoside, apigenin-7,4′-diglucoside, apigenin-7-digalacturonide, apigenin-7-galacturonylglucoside, apigenin-7-digalacturonide-4′-glucoside, apigenin-6,8-di-C-glucoside, luteolin-glucoside, luteolin-7-diglucoside, luteolin-7,3′-diglucoside, luteolin-7,4′-diglucoside and luteolin-7-galacturonide-4′-glucoside and chrysoeriol glycoside [El-Negoumy, S. I. et al. Grasas Aceites (Seville) 1989, 40 (2) 87-9].
There is a great interest and medical need for the improvement of bioavailability of a large number of drugs, which are (a) poorly bioavailable, (b) administered for long periods, (c) toxic and (d) expensive. Maximizing oral bioavailability is therapeutically important because the extent of bioavailability directly influences plasma concentrations and consequently therapeutic efficacy and dose related toxic effects resulting after oral drug administration. Poorly bioavailable drugs remain sub-therapeutic because a major portion of a dose never reaches the plasma or exerts its pharmacological effect unless and until very large doses are given which may lead to serious side effects. Any significant improvement in bioavailability will result in lowering the dose or the dose frequency of that particular drug. Besides, inter-subject variability is inversely correlated with the extent of bioavailability. Therefore, low oral bioavailability leads to high variability and poor control of plasma concentration and pharmacodynamic effects. Inter-subject variability is particularly of concern for a drug with a narrow safety margin.
Incomplete oral bioavailability has various causes. These include poor dissolution or low aqueous solubility, poor intestinal membrane permeation, degradation of the drug in gastric or intestinal fluids and pre-systemic intestinal or hepatic metabolism. The normal practice to offset some of these problems has been to increase the dosage as stated earlier, which has the concerns of toxicity patients' non-compliance.
Many therapeutic treatments are also accompanied by loss of essential nutraceuticals in the course of therapy. The present invention improves nutritional status by increasing bioavailability/bioefficacy of various nutraceuticals also, which include metals and vitamins. The bioenhancers of the invention also have the potential to enhance the bioefficacy of a drug without influencing its plasma concentrations for various reasons, some of which, but not limited to, are described later in this invention under Section on ‘Bioavailability/Bioenhancing activity’.
Bioavailability/Bioefficacy Enhancing Activity
In the present invention, the term Bioavailability or bioenhancing (BE) activity is defined as “a product at a lower dosage level which in combination with a drug or nutrient, provides more availability of the drug there by reducing the consumption of the drug or nutrient resulting in enhanced efficacy of the said drug.
In the present invention, the aqueous, aqueous—alcoholic, ketonic, ethereal, halogenated solvent extracts of the plant parts were evaluated with different therapeutic categories of drugs and nutraceutical (vital amino acids, metals, antioxidants, vitamins), and herbal drugs either alone or in combination. The bioavailability enhancing (BE) activity of the extracts was found to be consistent from 2.0 mg to 100 mg irrespective of the amount of the drug(s) present in the formulation. Sub-fractions of the extracts were also evaluated, with the same categories of drugs. The BE activity of the fractions increased corresponding to their proportions in the parent extract. The doses of the fractions responsible for the BE activity ranged from 0.5 to 25.0 mg. Both the fractions were found to be equally active within the above-mentioned range. Both the parent extracts as well as the fractions were found to be active individually as well as in combination with each other with different categories of drugs. The bioenhancer activity of the fraction(s) was found to be consistent from 0.5 mg to 25.0 mg irrespective of the amount of the drug(s) present in the formulation. The BE activity of the fractions was more enhanced as compared to that of the parent extracts.
The extracts or its fractions were found to be upto 50% more active when used individually in combination with piperine (1-piperoyl piperidine). Besides, both the parent extracts as wells as their fractions in different combinations showed almost similar enhanced activity upto 60% in presence of piperine. The amount of piperine in these formulations ranged from 3-20 mg.
The extracts or its fractions either in presence or absence of piperine have been found to be highly selective in their bioavailability enhancing activity. This is apparent from the degree of bioavailibility enhancement caused by these extracts/fractions. It varies from Nil to nearly significant (25%) to highly significant (435%). The reasons for this selective pattern may be attributable to one or more than one of the following reasons:
Primarily, but not exclusively, the invention enhances the carrier mediated entry of drugs and also the passive diffusion and the active transport pathways in the tissue which are responsible for transporting physiological substances such as nutraceuticals to their target sites. As applicable to any mechanism of action the products of this invention contribute in a synergistic and/or additive manner so that most drugs and nutraceuticals in presence of the products described in the present art are more bioavailable or bioefficaceous as a result of one or more of these mechanisms. The bioavailability and/or bioefficacy of drugs and nutraceuticals is also relevant to animal health besides being important for humans. The invention therefore is also intended to be used in veterinary preparations.
The main object of the invention is to provide an active of extract and bioactive fraction obtained from Cuminum cyminum.
Another object of the invention is to evaluate bioenhancing/bioavailability of Cuminum cyminum extract or bioactive fraction in combination with drugs, nutrients, nutraceuticals, micronutrients and herbal drugs/products.
Still another object of the invention is to provide a bioenhancer composition comprising active principles of Cuminum cyminum in combination with drugs, nutrients, nutraceuticals, micronutrients and herbal drugs/products.
Still another embodiment of the present invention is to provide a bioenhancer composition comprising extract and/or bioactive fractions obtained from Cuminum cyminum, piperine and one or more selected from the group consisting of drugs, nutrients, nutraceuticals, micronutrients and herbal drugs/products.
Yet, another object of the invention is to provide a process for isolating bioactive factions from Cuminum cyminum useful as a bioenhancer.
Yet, another object of the invention is to provide a process for isolating bioactive faction from Cuminum cyminum using aqueous and/or alcoholic solvent
Accordingly, the present invention is directed to preparation of active extracts/fraction from the plant Cuminum cyminum which include their chemical characterization, fingerprint profiling and methods of using such products to enhance bioavailability and/or bioefficacy of drugs, natural products and essential nutraceuticals. The present invention is directed to preparation of composite bioenhancers comprising polar and non-polar extracts of parts of Cuminum cyminum and/or piperine (Ex: Piper nigrum and Piper longum) which increased significantly (25 to 435%), the bioavailability of a number of classes of drugs, for example, but not limited to, antibiotics, antifungals, anti-virals, anticancer, cardiovascular, CNS, anti-inflammatory/anti-arthritic, anti-TB/anti leprosy, anti-histaminic/respiratory disorders, corticosteroids, immunosuppressants, anti-ulcer. Such extracts/fractions of C. cyminum either in presence or absence of piperine (Ex: Piper nigrum and Piper longum) have been found to be highly selective in their bioavailability/bioefficacy enhancing action.
Accordingly, the present invention provides a bioenhancing/bioavailability-facilitating composition comprising:
One embodiment of the invention the amount of Cuminum cyminum extract used is in the range of about 1.0 to 250 mg, preferably in the range of 2.0 to 100 mg.
Another embodiment, the amount of Cuminum cyminum fraction used is in the range of about 0.5 to 75.0 mg irrespective of the amount of additive selected from drugs, nutrients, vitamins, nutraceuticals, herbal drugs/products, micro nutrients and antioxidants, preferably in the range of about 1.0 to 30 mg. The fraction Cuminum cyminum used is selected from fractions identified as 1 to 5
The amount of piperine used is in the range of about 3 to 50 mg, more particularly between 3.to 20 mg, wherein the piperine is isolated from piper nigrum, piper longum or its oleoresin.
Another embodiment of the invention provides a composition in which the drugs are selected from the group consisting of antibiotics, antifungal, antiviral, anticancer, cardiovascular, CNS drugs, anti-inflammatory/anti-arthritic, anti-TB/anti-leprosy drugs, anti-histamines/drugs for respiratory disorders, corticosteriods, immuno-suppressants, anti-ulcer drugs and herbal drugs.
Still another embodiment, the antibiotic used is selected from the group consisting of quinolones, macrolides, cephalosproins, penicillin and aminoglycosides; the quinolone is selected from the group consisting of Ciprofloxacin, Pefloxacin, Ofloxacin and Norfloxacin; the macrolide is selected from the group consisting of Erythromycin, Roxythromycin and Azithromycin; the cephalosproins is selected from the group consisting of Cefalexin, cefatrioxone, cefixime, Cefpirome, Cefdinir and Cefadroxil; the penicillin is selected from the group consisting of Amoxycillin and Cloxacillin; and the aminoglycocide is selected from the group consisting of Amikacin and Kanamycin.
Still another embodiment, the anti-fungal drug is selected from the group consisting of Fluconazole, Amphotericin B, Griseofulvin and Ketoconazole and the antiviral drug is selected from the group consisting of Acyclovir and Zidovudine.
Yet another embodiment, the anticancer drug is selected from the group consisting of Methotrexate, 5-Fluorouracil, Doxorubicin, Taxol and Cisplatin.
Another embodiment, the cardiovascular drug is selected from the group consisting of Amlodipin, Lisinopril, propranolol and Atenolol and CNS drugs is selected from the group consisting of Alprazolam and Haloperidol
Still another embodiment anti-inflammatory/anti-arthritic drug is selected from the group consisting of Diclofenac, Piroxicam, Nimesulide and Rofecoxib and anti-TB/anti-leprosy drug is selected from the group consisting of Rifampicin, Ethionamide, Isoniazid, Cycloserine, Pyrazinamide, Ethambutol and Dapsone
The antihistamine/drugs for respiratory disorders compound is selected from the group consisting of Salbutamol, Theophylline, Bromhexine and Loratidine; the corticosteriod is selected from the group consisting of Prednisolone, dexamethasone and Betamethasone; immuno-supressant is selected from the group consisting of Cyclosporin A, Tacrolimus and Mycophenolatemofetil and the anti-ulcer compound is selected from the group consisting of Rantidine, Cimetidine and Omerprazole.
Yet another embodiment the herbal product/drug is selected from Echinacea, Tinospora cordifolia, Picrorrhiza kurroa, Aegles marmelos, Andrographis paniculata, Emblica ribes, Asparagus racemosus, Terminalia chebula Withania somnifera, Centella asiatica and/or their mixture thereof.
Another embodiment of the invention relates to a composition, wherein the nutrient is selected from group consisting of sugar, carbohydrates, fats and proteins, the vitamin is selected from the group consisting of Vitamin A, Vitamin E, Vitamin B1, Vitamin B6, Vitamin B12, Vitamin C and Folic acid; and the antioxidant is selected from the group consisting of B-Carotene, Silymarin, Selenium, Lycopene and Ellagiogallotannins
Still another embodiment, natural herbal product is selected from the group consisting of Curcumin, Boswellic acids and Rutin and essential micro nutrients is selected from the group consisting of Methionine, Lysine, Leucine, Valine, Isoleucine, Zinc, Calcium, Glucose, Potassium, Copper and Iron
Yet another embodiment, the plant extract of Cuminum cyminum or its bioactive fraction is extracted from any plant parts of Cuminum cyminum
In another embodiment, the above said composition is administered through oral, parenteral, nasal, inhalation including nebulisers, rectal, vaginal, transdermal and any others suitable routes.
In another embodiment, the bioenhancing effect of the extracts/fractions of Cuminum cyminum alone or in combination with piperine is selective in enhancing the bioavailability/bioefficacy of a drug, nutraceutical, and herbal drug/formulation.
One more embodiment of the invention provides the composition containing Cuminum cyminum extract or the fractions there of which provides bioavailability/bio-enhancing activity in the range of 25-335%
Still another embodiment, the composition comprising components of Cuminum cyminum and piperine exhibit nearly significant i.e. about 25% to highly significant i.e. about 435% bioenhancing/bioavailability activity.
Yet another embodiment, the said composition-containing piperine and Cuminum cyminum provides further bioavailability/bio-enhancing activity in the range of 10 to 85% more than bioenhancing activity of Cuminum cyminum alone.
Another embodiment of the invention relates to the dosage level of the composition comprising Cuminum cyminum extract is in the range of 10 to 30-mg/kg/body weight and composition comprising bioactive fraction is in the range of 2 to 20-mg/kg/body weight.
Another embodiment of the invention relates to the dosage level of the composition comprising Cuminum cyminum extract or bioactive fraction along with piperine, wherein the dosage of piperine is in the range of 0 to 12-mg/kg/body weight.
One more embodiment of the present invention provides a process for the preparation of an aqueous extract, aqueous alcoholic extract and bioactive fraction from the plant Cuminum cyminum, said process comprises steps of:
One more embodiment of the invention provides HPLC chromatogram for the extract and bioactive fractions obtained from plant Cuminum cyminum.
Still another embodiment, the HPLC chromatrogaph is obtained by using 2% acetic acid in water:acetonitrile in the ratio 83:17, RP18 column; flow rate 1 ml/minute using UV detector.
Another embodiment, the aqueous extract obtained from the plant Cuminum cyminum is having HPLC chromatograph major peaks with the retention time 2.16, 2.44, 4.40, 6.56, 8.27, 14.34 and 15.24.
Another embodiment, 50% aqueous alcoholic extract obtained from the plant Cuminum cyminum is having HPLC chromatograph peaks with the retention time 4.38, 6.53, 8.25, 10.43, 14.29 and 15.17.
Still another embodiment, fraction 1 obtained from the plant Cuminum cyminum is having HPLC chromatograph peaks with the retention time 2.52, 3.59, 6.25, 11.01 and 14.46.
Still another embodiment, fraction 2 obtained from the plant Cuminum cyminum has HPLC chromatograph peaks with the retention time 2.43, 4.38 and 6.52.
Still another embodiment, fraction 3 obtained from the plant Cuminum cyminum has HPLC chromatograph peaks with the retention time 3.11, 7.75, 10.37, 13.80 and 17.95.
Still another embodiment, fraction 4 obtained from the plant Cuminum cyminum has HPLC chromatograph major peaks with the retention time 2.31, 3.14, 5.25, 7.71, 9.63, 13.82 and 17.92.
Still another embodiment, fraction 5 obtained from the plant Cuminum cyminum has HPLC chromatograph major peaks with the retention time 2.25, 3.01, 5.21 and 7.69.
The following examples are intended to demonstrate some of the preferred embodiments and in no way should be construed so as to limit the scope of the invention. Any person skilled in the art can design more formulations, which may be considered as part of the present invention.
Preparation of colourless, non-pungent piperine by a novel process as already claimed in IP 1726890 and further modified as follows: Commercially available Piper nigrum or Piper longum or their oleoresins have been used as the source material. 20 kg long pepper oleoresin is extracted with chlorinated solvents like CHCl3, CH2Cl2, C2H4Cl2 (25 litre) for six hours or 20 kg black pepper powder is Soxhletted with toluene for 8 hours. The extracts are concentrated to dryness under reduced pressure and dissolved in ethanol at 78° C. The ethanolic solution is adsorbed over neutral Al2O3 and packed in a glass column. Elution is carried out with CHCl3: EtOH (9:1) and the eluate is concentrated to dryness and dissolved in minimum quantity of ethanol. The solution is treated with activated charcoal and filtered through a celite bed. The filtrate is concentrated to saturation point, cooled when colourless crystalline precipitate is obtained. The precipitate is separated by suction filtration and dried.
The specifications of the preferred materials are as under:
Doses, models/design of experiments and estimation methodology in a typical experiment is given below:
Doses of Different Bioenhancers Used and the Design of a Typical Experiment
As an example of an experiment in Rat (fasted):
Experimental procedure: Drug alone/or in combination with BE was administered to rats as per the following design:
Blood from control/treated animals at predetermined intervals (0-24 hrs) (Total 14 timings). Rifampicin was extracted from the blood (plasma) using dichloromethane. The concentration of rifampicin in the samples was determined using HPLC (Model: Shimadzu 1080 BP) ; PDA detector; Mobile phase : phosphate buffer: acetonitile (40:60); Flow rate 1.0 ml/min. Column RP 18.
Control and BE only groups were employed to study the interference of plasma component and the bioenhancer used.
The above methodology was adapted for evaluating the bio-enhancing activity of other drugs, micro nutrients, nutracuticals, nutrients and other herbal products and the enhancing effects are tabulated under each heading. List of drugs, nutraceuticals, herbal formulations cited below as some of the example for the purpose of present invention.
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum
Cuminum cyminum.
Cuminum cyminum.
Cuminum cyminum
Cuminum cyminum.
Cuminum cyminum.
Cuminum
cyminum
C. cyminum
Cuminum cyminum.
C. cyminum
Echinacea
Tinospora cordifolia
Picrorrhiza kurroa
Aegles marmelos
Andrographis paniculata
Emblica ribes
Asparagus racemosus
Terminalia chebula
Withania somnifera
Centella asiatica
Flow Sheet for Fractionation of Aqueous Extract of Cuminum Cyminum
Bioactivity Guided Fractionation of Aqueous Extract was Carried out as Typical Example by Partitioning with n-BuOH and H2O (Scheme 1) and Triturating Another Portion of Extract with 95% EtOH, 50% Aq. EtOH (Scheme-2)
The original 50% aq. alcoholic extract of the plant material can also be fractionated by the same scheme as above.
The original 50% aq. alcoholic extract of the plant material can also be fractionated by scheme as mentioned above.
This application is a divisional of application Ser. No. 10/386,395 filed Mar. 11, 2003 now U.S. Pat. No. 7,070,814. The nonprovisional application designated above, namely application Ser. No. 10/386,395 filed on Mar. 11, 2003, claims the benefit of U.S. Provisional Application No. 60/363,460 filed Mar. 12, 2002 and incorporates the same by reference.
| Number | Name | Date | Kind |
|---|---|---|---|
| 5482711 | Medenica | Jan 1996 | A |
| 20010055625 | Katiyar et al. | Dec 2001 | A1 |
| 20030170321 | Oazi et al. | Sep 2003 | A1 |
| 20030194456 | Arora et al. | Oct 2003 | A1 |
| 20040121028 | Qazi et al. | Jun 2004 | A1 |
| 20040198672 | Qazi et al. | Oct 2004 | A1 |
| Number | Date | Country | |
|---|---|---|---|
| 20060141080 A1 | Jun 2006 | US |
| Number | Date | Country | |
|---|---|---|---|
| 60363460 | Mar 2002 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 10386395 | Mar 2003 | US |
| Child | 11360653 | US |
