Claims
- 1. A device for measuring a set of biological analytes, comprising:
a substrate; a plurality of enhancing particle structures on said substrate; and a set of receptor types, each receptor type being specific for one member of said set of biological analytes, each of said receptor types being spatially separated from other receptor types on said substrate.
- 2. The device of claim 1, further comprising a passivation agent associated with said substrate or said plurality of enhancing structures.
- 3. The device of claim 1, wherein each of said receptor types is specific for an analyte selected from the group consisting of cAMP, serotonin, monoamine oxidase I, monoamine oxidase II, beta-amyloid peptide, Tau protein, PrPSc, ATP, ADP, AMP, enzymes involved in energy metabolism, calmodulin, calmodulin binding protein, heat-shock proteins, superoxide dismutase, glutathione peroxidase, reduced and oxidized glutathione, nitrotyrosine, FADH, NADH, pyruvate, acetyl Co-A, GTP, NADPH, NADPH oxido-reductase, catalase, cytochrome-A, cytochrome-B, cytochrome-C, beta-hydroxybutyrate, acetylacetate, lactate, glycerol 3-phosphate, glucose 6-phosphate, creatine phosphate, 1,3-diphosphoglycerate, phosphoenolpyruvic acid, acetylphosphate, UTP, CTP, dATP, dGTP, dTTP and dCTP.
- 4. A device for measuring an analyte, comprising:
a substrate; an enhancing particle structure; and a receptor specific for a carcinophore.
- 5. The device of claim 4, wherein said carcinophore is a metalloproteinase.
- 6. The device of claim 4, wherein said carcinophore is selected from the group consisting of Pro MMP-1, MMP-1, Pro MMP-9, MMP-9, MT1-MMPT, Pro MMP-2, MMP-2, TNF-alpha, IL-1 beta, IL2, VEGF, TIMP-1, TIMP-2, TIMP-3, Pro-cathepsin, cathepsin B and plasmin.
- 7. The device of claim 2, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of cytochrome-C, Bak, Bax, Bcl-x, Bcl-1, Bcl-2, p-53, caspase-2, caspase-3, PARP, and WAF-1.
- 8. The device of claim 2, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of ATP, ADP, AMP, creatine, creatine-phosphate, lactic acid, acetyl-CoA, NADH, NAD+, NADPH, NADP+, GTP, total mitochondria, B-OHb and AcA.
- 9. The device of claim 2, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of Hsp27, Hsp70, Hsp0, Hsp90, malondialdehyde, nitrosotyrosine, nitric oxide synthetase, superoxide dismutase, glutathione peroxidase, glutathione reductase, cyclooxygenase 1, cyclooxygenase 2, a thromboxane, a prostaglandin, prostaglandin H synthetase, isoprostane ipF2α-VI, heme oxygenase and catalase.
- 10. The device of claim 2, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-α, C-reactive protein, ceruloplasmin, MTP, a prostaglandin, a leukotriene and a glucocorticoid.
- 11. The device of claim 2, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of white blood cells, TNF-α, insulin, proinsulin, Apo-A, ferritin, a VLDL, a FFA, glucose, and an HDL.
- 12. The device of claim 2, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of cathepsin-B, cathepsin-L, cathepsin-D, MMP-1, MMP-2, MMP-9, MMP-13, α2-macroglobulin, and TIMP-1.
- 13. The device of claim 1, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of cytochrome-C, Bak, Bax, Bcl-x, Bcl-1, Bcl-2, p-53, caspase-2, caspase-3, PARP, and WAF-1.
- 14. The device of claim 1, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of ATP, ADP, AMP, creatine, creatine-phosphate, lactic acid, acetyl-CoA, NADH, NAD+, NADPH, NADP+, GTP, total mitochondria, B-OHb and AcA.
- 15. The device of claim 1, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of Hsp27, Hsp70, Hsp0, Hsp90, malondialdehyde, nitrosotyrosine, nitric oxide synthetase, superoxide dismutase, glutathione peroxidase, glutathione reductase, cyclooxygenase 1, cyclooxygenase 2, a thromboxane, a prostaglandin, prostaglandin H synthetase, isoprostane ipF2α-VI, heme oxygenase and catalase.
- 16. The device of claim 1, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-α, C-reactive protein, ceruloplasmin, MTP, a prostaglandin, a leukotriene and a glucocorticoid.
- 17. The device of claim 1, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of white blood cells, TNF-α, insulin, proinsulin, Apo-A, ferritin, a VLDL, a FFA, glucose, and an HDL.
- 18. The device of claim 1, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of cathepsin-B, cathepsin-L, cathepsin-D, MMP-1, MMP-2, MMP-9, MMP-13, α2-macroglobulin, and TIMP-1.
- 19. The device of claim 1, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of cytochrome-A, cytochrome-B, cytochrome-C, Bak, Bax, Bcl-x, Bcl-1, Bcl-2, p-53, caspase-2, caspase-3, PARP, and WAF-1, ATP, ADP, AMP, creatine, creatine-phosphate, lactic acid, acetyl-CoA, NADH, NAD+, NADPH, NADP+, GTP, total mitochondria, B-OHb and AcA, Hsp27, Hsp70, Hsp0, Hsp90, malondialdehyde, nitrosotyrosine, nitric oxide synthetase, superoxide dismutase, glutathione peroxidase, glutathione reductase, cyclooxygenase 1, cyclooxygenase 2, a thromboxane, a prostaglandin, prostaglandin H synthetase, isoprostane ipF2α-VI, heme oxygenase and catalase, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-α, C-reactive protein, ceruloplasmin, MTP, a prostaglandin, a leukotriene and a glucocorticoid, white blood cells, TNF-α, insulin, proinsulin, Apo-A, ferritin, a VLDL, a FFA, glucose, and a HDL, cathepsin-B, cathepsin-L, cathepsin-D, MMP-1, MMP-2, MMP-9, MMP-13, α2-macroglobulin, and TIMP-1, cAMP, serotonin, monoamine oxidase I, monoamine oxidase II, beta-amyloid peptide, Tau protein, PrPSc, calmodulin, calmodulin binding protein, FADH, pyruvate, GTP, NADPH oxido-reductase, catalase, lactate, glycerol 3-phosphate, glucose 6-phosphate, 1,3diphosphoglycerate, phosphoenolpyruvic acid, acetylphosphate, UTP, CTP, dATP, dGTP, dTTP, dCTP, Pro MMP-1, Pro MMP-9, MT1-MMPT, Pro MMP-2, TNF-alpha, IL-1 beta, VEGF, TIMP-2, TIMP-3, Pro-cathepsin, cathepsin B and plasmin.
- 20. A device for measuring an analyte, comprising:
a substrate; at least one enhancing particle structure on said substrate; and at least one receptor type specific for an analyte selected from the group consisting of cytochrome-A, cytochrome-B, cytochrome-C, Bak, Bax, Bcl-x, Bcl-1, Bcl-2, p-53, caspase-2, caspase-3, PARP, and WAF-1, ATP, ADP, AMP, creatine, creatine-phosphate, lactic acid, acetyl-CoA, NADH, NAD+, NADPH, NADP+, GTP, total mitochondria, B-OHb and AcA, Hsp27, Hsp70, Hsp0, Hsp90, malondialdehyde, nitrosotyrosine, nitric oxide synthetase, superoxide dismutase, glutathione peroxidase, glutathione reductase, cyclooxygenase 1, cyclooxygenase 2, a thromboxane, a prostaglandin, prostaglandin H synthetase, isoprostane ipF2α-VI, heme oxygenase and catalase, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-α, C-reactive protein, ceruloplasmin, MTP, a prostaglandin, a leukotriene and a glucocorticoid, white blood cells, TNF-α, insulin, proinsulin, Apo-A, ferritin, a VLDL, a FFA, glucose, and a HDL, cathepsin-B, cathepsin-L, cathepsin-D, MMP-1, MMP-2, MMP-9, MMP-13, α2-macroglobulin, and TIMP-1, cAMP, serotonin, monoamine oxidase I, monoamine oxidase II, beta-amyloid peptide, Tau protein, PrPSc, calmodulin, calmodulin binding protein, FADH, pyruvate, GTP, NADPH oxido-reductase, catalase, lactate, glycerol 3-phosphate, glucose 6-phosphate, 1,3diphosphoglycerate, phosphoenolpyruvic acid, acetylphosphate, UTP, CTP, dATP, dGTP, dTTP, dCTP, Pro MMP-1, Pro MMP-9, MT1-MMPT, Pro MMP-2, TNF-alpha, IL-1 beta, VEGF, TIMP-2, TIMP-3, Pro-cathepsin, cathepsin B and plasmin.
- 21. The device of claim 2, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of cytochrome-A, cytochrome-B, cytochrome-C, Bak, Bax, Bcl-x, Bcl-1, Bcl-2, p-53, caspase-2, caspase-3, PARP, and WAF-1, ATP, ADP, AMP, creatine, creatine-phosphate, lactic acid, acetyl-CoA, NADH, NAD+, NADPH, NADP+, GTP, total mitochondria, B-OHb and AcA, Hsp27, Hsp70, Hsp0, Hsp90, malondialdehyde, nitrosotyrosine, nitric oxide synthetase, superoxide dismutase, glutathione peroxidase, glutathione reductase, cyclooxygenase 1, cyclooxygenase 2, a thromboxane, a prostaglandin, prostaglandin H synthetase, isoprostane ipF2α-VI, heme oxygenase and catalase, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-α, C-reactive protein, ceruloplasmin, MTP, a prostaglandin, a leukotriene and a glucocorticoid, white blood cells, TNF-α, insulin, proinsulin, Apo-A, ferritin, a VLDL, a FFA, glucose, and a HDL, cathepsin-B, cathepsin-L, cathepsin-D, MMP-1, MMP-2, MMP-9, MMP-13, α2-macroglobulin, and TIMP-1, cAMP, serotonin, monoamine oxidase I, monoamine oxidase II, beta-amyloid peptide, Tau protein, PrPSc, calmodulin, calmodulin binding protein, FADH, pyruvate, GTP, NADPH oxido-reductase, catalase, lactate, glycerol 3-phosphate, glucose 6-phosphate, 1,3-diphosphoglycerate, phosphoenolpyruvic acid, acetylphosphate, UTP, CTP, dATP, dGTP, dTTP, dCTP, Pro MMP-1, Pro MMP-9, MT1-MMPT, Pro MMP-2, TNF-alpha, IL-1 beta, VEGF, TIMP-2, TIMP-3, Pro-cathepsin, cathepsin B and plasmin.
- 22. The device of claim 1, wherein said set of receptor types includes at least two receptor types, each of said receptor types specific for an analyte selected from the group consisting of cytochrome-A, cytochrome-B, cytochrome-C, Bak, Bax, Bcl-x, Bcl-1, Bcl-2, p-53, caspase-2, caspase-3, PARP, and WAF-1, ATP, ADP, AMP, creatine, creatine-phosphate, lactic acid, acetyl-CoA, NADH, NAD+, NADPH, NADP+, GTP, total mitochondria, B-OHb and AcA, Hsp27, Hsp70, Hsp0, Hsp90, malondialdehyde, nitrosotyrosine, nitric oxide synthetase, a superoxide dismutase, glutathione peroxidase, glutathione reductase, cyclooxygenase 1, cyclooxygenase 2, a thromboxane, a prostaglandin, prostaglandin H synthetase, isoprostane ipF2α-VI, heme oxygenase and catalase, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-α, C-reactive protein, ceruloplasmin, MTP, a prostaglandin, a leukotriene, a glucocorticoid, white blood cells, TNF-α, insulin, proinsulin, Apo-A, ferritin, a VLDL, a FFA, glucose, an HDL, cathepsin-B, cathepsin-L, cathepsin-D, MMP-1, MMP-2, MMP-9, MMP-13, α2-macroglobulin, TIMP-1, cAMP, serotonin, monoamine oxidase I, monoamine oxidase II, beta-amyloid peptide, Tau protein, PrPSc, calmodulin, calmodulin binding protein, FADH, pyruvate, GTP, NADPH oxido-reductase, catalase, lactate, glycerol 3-phosphate, glucose 6-phosphate, 1,3-diphosphoglycerate, phosphoenolpyruvic acid, acetylphosphate, UTP, CTP, dATP, dGTP, dTTP, dCTP, Pro MMP-1, Pro MMP-9, MT1-MMPT, Pro MMP-2, TNF-alpha, IL-1 beta, VEGF, TIMP-2, TIMP-3, Pro-cathepsin, cathepsin B and plasmin.
- 23. A method for analyzing a biological process, comprising:
(a) providing a substrate having:
a plurality of enhancing particle structures on said substrate; and at least one receptor type associated with said enhancing particle structures, said receptor type being specific for a bioanalyte characteristic of said biological process; (b) placing a biological sample containing said bioanalyte in contact with said receptors; and (c) detecting and/or quantifying the presence of said bioanalyte.
- 24. The method of claim 23, further comprising, after step (b), washing unbound analyte from said substrate.
- 25. The method of claim 23, wherein said bioanalyte is selected from the group consisting of cytochrome-A, cytochrome-B, cytochrome-C, Bak, Bax, Bcl-x, Bcl-1, Bcl-2, p-53, caspase-2, caspase-3, PARP, and WAF-1, ATP, ADP, AMP, creatine, creatine-phosphate, lactic acid, acetyl-CoA, NADH, NAD+, NADPH, NADP+, GTP, total mitochondria, B-OHb and AcA, Hsp27, Hsp70, Hsp0, Hsp90, malondialdehyde, nitrosotyrosine, nitric oxide synthetase, superoxide dismutase, glutathione peroxidase, glutathione reductase, cyclooxygenase 1, cyclooxygenase 2, a thromboxane, a prostaglandin, prostaglandin H synthetase, isoprostane ipF2α-VI, heme oxygenase and catalase, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-α, C-reactive protein, ceruloplasmin, MTP, a prostaglandin, a leukotriene and a glucocorticoid, white blood cells, TNF-α, insulin, proinsulin, Apo-A, ferritin, a VLDL, a FFA, glucose, and a HDL, cathepsin-B, cathepsin-L, cathepsin-D, MMP-1, MMP-2, MMP-9, MMP-13, α2-macroglobulin, and TIMP-1, cAMP, serotonin, monoamine oxidase I, monoamine oxidase II, beta-amyloid peptide, Tau protein, PrPSc, calmodulin, calmodulin binding protein, FADH, pyruvate, GTP, NADPH oxido-reductase, catalase, lactate, glycerol 3-phosphate, glucose 6-phosphate, 1,3-diphosphoglycerate, phosphoenolpyruvic acid, acetylphosphate, UTP, CTP, dATP, dGTP, dTTP, dCTP, Pro MMP-1, Pro MMP-9, MT1-MMPT, Pro MMP-2, TNF-alpha, IL-1 beta, VEGF, TIMP-2, TIMP-3, Pro-cathepsin, cathepsin B and plasmin.
- 26. The method of claim 23, wherein said substrate has at least one additional receptor type specific for a different bioanalyte of said set of bioanalytes.
- 27. The method of claim 26, wherein said additional bioanalyte is selected from the group consisting of cytochrome-A, cytochrome-B, cytochrome-C, Bak, Bax, Bcl-x, Bcl-1, Bcl-2, p-53, caspase-2, caspase-3, PARP, and WAF-1, ATP, ADP, AMP, creatine, creatine-phosphate, lactic acid, acetyl-CoA, NADH, NAD+, NADPH, NADP+, GTP, total mitochondria, B-OHb and AcA, Hsp27, Hsp70, Hsp0, Hsp90, malondialdehyde, nitrosotyrosine, nitric oxide synthetase, superoxide dismutase, glutathione peroxidase, glutathione reductase, cyclooxygenase 1, cyclooxygenase 2, a thromboxane, a prostaglandin, prostaglandin H synthetase, isoprostane ipF2α-VI, heme oxygenase and catalase, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-α, C-reactive protein, ceruloplasmin, MTP, a prostaglandin, a leukotriene and a glucocorticoid, white blood cells, TNF-α, insulin, proinsulin, Apo-A, ferritin, a VLDL, a FFA, glucose, and a HDL, cathepsin-B, cathepsin-L, cathepsin-D, MMP-1, MMP-2, MMP-9, MMP- 13, α2-macroglobulin, and TIMP-1, cAMP, serotonin, monoamine oxidase I, monoamine oxidase II, beta-amyloid peptide, Tau protein, PrPSc, calmodulin, calmodulin binding protein, FADH, pyruvate, GTP, NADPH oxido-reductase, catalase, lactate, glycerol 3-phosphate, glucose 6-phosphate, 1,3-diphosphoglycerate, phosphoenolpyruvic acid, acetylphosphate, UTP, CTP, dATP, dGTP, dTTP, dCTP, Pro MMP-1, Pro MMP-9, MT1-MMPT, Pro MMP-2, TNF-alpha, IL-1 beta, VEGF, TIMP-2, TIMP-3, Pro-cathepsin, cathepsin B and plasmin.
- 28. A method for manufacturing a biochip, comprising:
(a) providing a substrate; (b) attaching a plurality of enhancing particle structures to said substrate; and (c) attaching at least one receptor to said biochip, said receptor associated with at least one of said particle structures, said receptor adapted to associate with a bioanalyte characteristic of a biological process.
- 29. The method of claim 28, further comprising attaching at least one additional receptor to said biochip, said at least one additional receptor adapted to associated with another bioanalyte characteristic of said biological process.
- 30. The method of claim 29, further comprising passivating one or more of said substrate and said plurality of enhancing particle structures.
- 31. The device of claim 1, wherein said enhancing particle structures are fractal structures.
- 32. The device of claim 2, wherein said enhancing particle structures are fractal structures.
- 33. The device of claim 4, wherein said enhancing particle structures are fractal structures.
- 34. The device of claim 20, wherein said enhancing particle structures are fractal structures.
- 35. The method of claim 23, wherein said enhancing particle structures are fractal structures.
- 36. The method of claim 28, wherein said enhancing particle structures are fractal structures.
- 37. A device for measuring a set of biological analytes, comprising:
a substrate; means for enhancing a Raman signal of one of said biological analytes on said substrate; and a set of receptor types, each receptor type being specific for one member of said set of biological analytes, each of said receptor types being spatially separated from other receptor types on said substrate.
- 38. The device of claim 37, further comprising a passivating agent associated with said substrate or said plurality of enhancing structures.
- 39. A method for analyzing a biological process, comprising:
(a) providing a substrate having:
means for enhancing a Raman signal of a bioanalyte characteristic of said biological process; and at least one receptor type associated with said means for enhancing a Raman signal, said receptor type being specific for said bioanalyte; (b) placing a biological sample containing said bioanalyte in contact with said receptors; and (c) detecting and/or quantifying the presence of said bioanalyte.
- 40. The method of claim 39, further comprising providing said substrate with at least one additional receptor type associated with said means for enhancing a Raman signal, said receptor type being specific for another bioanalyte characteristic of said biological process.
RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. application Ser. No. 09/925,189, which is a continuation-in-part of U.S. application, Ser. No. 09/815,909 and U.S. application Ser. No. 09/670,453, filed Sep. 26, 2000, which claimed priority under 35 U.S.C. § 119 to U.S. Provisional Patent Application Serial No. 60/156,195, now abandoned. This application also claims priority to U.S. Provisional Patent Application Serial No. 60/336,445, filed Nov. 14, 2001. This application is related to U.S. patent application Ser. No. 09/669,369, filed Sep. 26, 2000, and U.S. patent application Ser. No. 09/669,796, filed Sep. 26, 2000. Each of these Patent Applications is herein incorporated fully by reference.
Provisional Applications (2)
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Number |
Date |
Country |
|
60156195 |
Sep 1999 |
US |
|
60336445 |
Nov 2001 |
US |
Continuation in Parts (3)
|
Number |
Date |
Country |
Parent |
09925189 |
Aug 2001 |
US |
Child |
10294385 |
Nov 2002 |
US |
Parent |
09815909 |
Mar 2001 |
US |
Child |
09925189 |
Aug 2001 |
US |
Parent |
09670453 |
Sep 2000 |
US |
Child |
09925189 |
Aug 2001 |
US |