Claims
- 1. A biologically active toluene monooxygenase enzyme from Pseudomonas mendocina KR-1, wherein the enzyme comprises TmoA, TmoB, TmoC, TmoD, and TmoE proteins, wherein said enzyme is free from association with other Pseudomonas mendocina KR-1 proteins, and wherein said enzyme has been produced by expression of the tmoA, tmoB, tmoC, tmoD, and tmoE genes in a microorganism other than P. mendocina.
- 2. The enzyme of claim 1, wherein said proteins have the amino acid sequences represented in FIG. 5.
- 3. A biologically active toluene monooxygenase enzyme from Pseudomonas mendocina KR-1, wherein the enzyme comprises TmoA, TmoB, TmoC, TmoD, TmoE, and TmoF proteins, wherein said enzyme is free from association with other Pseudomonas mendocina KR-1 proteins, and wherein said enzyme has been produced by expression of the tmoA, tmoB, tmoC, tmoD, tmoE, and tmoF genes in a microorganism other than P. mendocina.
- 4. The enzyme of claim 3, wherein said proteins have the amino acid sequences represented in FIG. 5.
Parent Case Info
This application is a continuation of application Ser. No. 07/960,025, filed Oct. 13, 1992, now abandoned, which is a division of application Ser. No. 07/590,374, filed Sep. 28, 1990, now U.S. Pat. No. 5,171,684, which is a continuation-in-part of application Ser. No. 07/177,631, filed Apr. 5, 1988, now U.S. Pat. No. 5,017,495.
Non-Patent Literature Citations (3)
Entry |
Whited, G. M. et al. 1991. Journal of Bacteriology, vol. 173, pp. 3010-3016. |
Whited, G. M. et al. 1991. Journal of Bacteriology, vol. 173, pp. 3017-3020. |
Whited Dissertatian Abstracts International vol. 47, No 5 p. 1861B, Nov. 1986. |
Divisions (1)
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590374 |
Sep 1990 |
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Continuations (1)
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Date |
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960025 |
Oct 1992 |
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Continuation in Parts (1)
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177631 |
Apr 1988 |
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