BIOENGINEERED VOCAL FOLD MUCOSA FOR FUNCTIONAL VOICE RESTORATION

Abstract

An engineered vocal fold mucosa, including an engineered lamina propria layer and an engineered squamous epithelium layer, is disclosed. The engineered lamina propria is made by seeding and culturing human vocal fold fibroblasts within a polymerized collagen scaffold, and the engineered squamous epithelium is made by culturing human vocal fold epithelial cells on the scaffold surface. The resulting engineered vocal fold mucosa is not immunogenic, and is capable of exhibiting the vibratory function and acoustic output of a native vocal fold mucosa. Accordingly, the engineered vocal fold mucosa may be implanted into the larynx to treat voice impairment.

Description

BACKGROUND

The human vocal folds (VFs, sometimes referred to as vocal cords or vocal chords), which are essential to the production of speech (phonation), are made up of a pair of structures that are stretched horizontally across the top of the trachea, within the larynx. The VF inner region of is made up of the vocalis muscle, which has both passive and active mechanical properties. Passively, the vocalis muscle has a relatively stiff consistency (sometimes compared to the consistency of a stiff rubber band), while, as a muscle, its active contractile properties help control its precise location and level of stiffness.

The vocalis muscle is covered by two mechanically de-coupled regions, each containing multiple layers. The region making up the outer covering of the vocal fold is known as the vocal fold mucosa (VFM). The vocal fold mucosa includes the outermost squamous epithelium layer and a mucosal lamina propria layer directly underneath the squamous epithelium. The squamous epithelium, which is composed primarily of stratified vocal fold epithelial cells (VFEs), serves as an initial boundary of protection for the underlying tissue and helps regulate vocal fold hydration. The underlying mucosal lamina propria, which is composed primarily of loose fibrous and elastic components in a vascularized matrix that is populated by vocal fold fibroblasts (VFEs), provides a pliant cushion having the mechanical properties needed for the vocal folds to vibrate in a manner that facilitates phonation.

The vocal folds also include a third region interposed between the inner vocalis muscle and the outer mucosa: the vocal ligament. The vocal ligament, which includes two non-mucosal lamina propria layers (the intermediate lamina propria and the deep lamina propria), is composed primarily of elastic and collagenous fibers, which provide this intermediate region with its elastic mechanical integrity and durability. See, e.g., Hirano M. Structure and vibratory behavior of the vocal fold, in Sawashima M, Cooper F (eds) (1977), Dynamic aspects of speech production, University of Tokyo, Tokyo, Japan: 13-30.

When inhaling, the vocal folds are separated, to facilitate the free flow of air through the trachea. When holding one's breath, the vocal folds tighten and come together, completely shutting off the free flow of air through the trachea. During phonation, the vocal folds are in an intermediate position, and the controlled passage of air from the lungs through the trachea causes the mucosa of adjoining vocal folds in contact with each other to vibrate at a high frequency. This transduction of energy from air flow from the lungs into high frequency vocal fold vibration in turn results in airborne sound waves that can be heard as speech. See, e.g., Matsushita H. The vibratory mode of the vocal folds in the excised larynx, Folia Phoniatr (Basel) 27 (1975): 7-18. Accordingly, phonation requires that both vocal fold mucosae are biomechanically capable of aerodynamic-to-acoustic energy transfer and high-frequency vibration, and physiologically capable of maintaining a barrier against the airway lumen.

Voice impairment (dysphonia) affects an estimated 20 million people in the United States, resulting in reduced general and disease-specific quality of life¹, reduced occupational performance and attendance^2,3, and direct health care costs exceeding $11 billion per year^4,5. Between 60 and 80% of voice complaints in the treatment-seeking population involve changes to the vocal fold (VF) mucosa⁶; severe mucosal impairment or loss due to trauma, disease, or disease resection often culminates in fibrosis and deterioration of VF vibratory capacity for voice⁷.

Patients with significant VF mucosal damage have limited treatment options. Medialization of the impaired VF, achieved by delivering an implant or injectate to the paraglottic space^8-10, can improve VF closure and therefore voice, but does not address fibrotic changes within the extracellular matrix (ECM). Superficial injection of regenerative biomaterials offers an alternative means to improve VF viscoelasticity and vibratory function^11,12; however, most biomaterials are not specifically engineered for the VF biomechanical environment, have limited residence time, and are not suited for large deficits involving extensive tissue loss. Creation of an organotypic bioengineered VF mucosa could theoretically bypass these challenges by providing on-demand tissue for transplantation that is both biomechanically appropriate for use as a dynamic sound source for voice production and capable of maintaining barrier function at the boundary of the upper and lower airways.

Tissue engineering of partial and complete VF mucosae has been attempted using decellularized ECM-based¹³ and collagen^14,15 and fibril^16,17 gel-based scaffolds, seeded with embryonic stem cell derivatives¹⁵, adult stem cells^16,17, and terminally differentiated cells^13,14,18. These organotypic culture approaches have generated engineered mucosae with desirable histologic features; however, to date, there is no benchmark culture system based solely on human-sourced VF cells against which stem cell-based approaches can be evaluated, no direct comparisons showing equivalency with native human VF mucosa, and most importantly, limited progress towards the restoration of physiologic function¹⁷. Significant advances have been hampered by the near-unavailability of disease-free primary human VF mucosal cells¹⁹ and limited attention to the intricate protein- and anatomic substructure-level complexity that characterizes mucosal morphogenesis.

Accordingly, there is a need for improved non-immunogenic transplantable engineered mucosae that are biomechanically capable of aerodynamic-to-acoustic energy transfer and high-frequency vibration, and physiologically capable of maintaining a barrier against the airway lumen.

BRIEF SUMMARY

This application discloses engineered vocal fold mucosae made from isolated and purified human VF fibroblasts (VFF) and human VF epithelial cells (VFE) co-cultured under organotypic conditions. The engineered vocal fold mucosae show morphologic features of native tissue. Specifically, the engineered vocal fold mucosae include an engineered mucosal lamina propria layer comprising a collagen polymer matrix populated by human VFFs in contact with an engineered outer squamous epithelium layer made from cultured human VFEs. The engineered vocal fold mucosae show proteome-level evidence of mucosal morphogenesis and emerging extracellular matrix complexity, and rudimentary barrier function in vitro. When grafted into larynges ex vivo, the engineered vocal fold mucosae generate physiologically appropriate vibratory behavior and acoustic output that are indistinguishable from those of native VF tissue. When grafted into humanized mice in vivo, the mucosae survive and are well tolerated by the human adaptive immune system. These results show that the disclosed compositions and methods can be used to restore voice function in patients with otherwise untreatable VF mucosal disease or damage.

In a first aspect, the disclosure encompasses an engineered vocal fold mucosa that includes (a) an engineered non-vascularized lamina propria made up of a scaffold of polymerized collagen populated by a plurality of human vocal fold fibroblasts (VFFs); and (b) an engineered stratified squamous epithelium that includes a plurality of human vocal fold epithelial cells (VFEs) that is in contact with the engineered non-vascularized lamina propria. The engineered vocal fold mucosa is capable of exhibiting the vibratory function and acoustic output of a native vocal fold mucosa.

As used herein, the term “scaffold” refers to a polymerized collagen that forms a gel-like composition. The scaffold is not impervious to aqueous solutions and other liquids, which can readily flow into the scaffold. For example, if the cell-populated scaffold is immersed in a culture medium, the culture medium may be absorbed into the scaffold and come in contact with the cells populating the scaffold. In the engineered vocal fold mucosa, the boundaries of the scaffold within which the VFFs reside also define the boundaries of the engineered non-vascularized lamina propria. Accordingly, the outer boundary of the scaffold is referred to herein as the “surface” of the scaffold.

The engineered stratified squamous epithelium (and the VFEs comprising the engineered squamous epithelium) is outside of the scaffold, and thus has an inner boundary at the scaffold surface. The engineered stratified squamous epithelium extends outward from the scaffold surface to its outermost boundary, the epithelial surface or luminal epithelial surface. The squamous epithelium region that is closest to the inner boundary at the scaffold surface is known as the basal surface region, and the squamous epithelium region that is closest to the luminal epithelial surface is known as the epithelial surface region. As in native VF mucosae, in the engineered VF mucosae, the VFE in the basal region form processes that extend into the underlying lamina propria, consistent with epithelial anchoring and barrier-like structure formation.

By “human vocal fold fibroblasts” (VFFs), we mean fibroblasts that are isolated from the vocal fold mucosa of a human (i.e., primary human vocal fold fibroblasts), or cells that are descended from such isolated human vocal fold mucosa fibroblasts or their progeny. Accordingly, the term is not limited to primary human vocal fold fibroblasts, but also encompasses cells resulting from the continued culturing, proliferation and/or passaging of cells derived from primary human vocal fold fibroblasts or their progeny. By “isolated,” we mean that the cells that have been removed from the native environment in which they originally resided. In the case of primary human vocal fold fibroblasts, the isolated cells are removed from the native extracellular matrix. The vocal fold mucosa from which the cells are isolated may have been obtained from a living human or from a human cadaver. In the case of a human cadaver, the vocal fold mucosa is preferably obtained less than six hours post-mortem.

By “human vocal fold epithelial cells” (VFEs), we mean epithelial cells that are isolated from the vocal fold mucosa of a human (i.e., primary human vocal fold epithelial cells), or cells that are descended from such isolated human vocal fold mucosa epithelial cells or their progeny. Accordingly, the term is not limited to primary human vocal fold epithelial cells, but also encompasses cells resulting from the continued culture, proliferation and/or passaging of cells derived from primary human vocal fold epithelial cells or their progeny. By “isolated,” we mean that the cells that have been removed from the native environment in which they originally resided. In the case of primary human vocal fold epithelial cells, the isolated cells are removed from the native squamous epithelium. The vocal fold mucosa from which the cells are isolated may have been obtained from a living human or from a human cadaver. In the case of a human cadaver, the vocal fold mucosa is preferably obtained less than six hours post-mortem.

The human vocal fold fibroblasts and human vocal fold epithelial cells do not include cells isolated from non-vocal fold mucosal tissue, such as cells isolated from generalized laryngeal mucosa or oral mucosa, or cells isolated from non-human subjects.

In some embodiments, the polymerized collagen is polymerized collagen, type I.

In some embodiments, the density of the VFFs populating the engineered non-vascularized lamina propria is from 100-300 cells/mm² or from 130-270 cells/mm².

In some embodiments, the engineered stratified squamous epithelium is between 30 and 70 um thick.

In some embodiments, one or more VFEs at both the basal and epithelial surface regions of the engineered stratified squamous epithelium express the basement membrane marker collagen, type IV.

In some embodiments, the engineered stratified squamous epithelium includes one or more Keratin 5⁺ VFEs, and the basal region of the engineered stratified squamous epithelium does not have a higher percentage of Keratin 5⁺ VFEs than the stratified squamous epithelium as a whole.

In some embodiments, one or more of the proteins listed in Table 1 below as being present in the engineered VF mucosa are included in the engineered VF mucosa. In some such embodiments, at least one of the included proteins is a protein that is not present in native VF mucosa.

In a second aspect, the disclosure encompasses an engineered vocal fold mucosa as described herein for use in treating voice impairment caused by vocal fold fibrosis or vocal fold mucosal tissue damage or loss.

In a third aspect, the disclosure encompasses an engineered vocal fold mucosa as described herein for use in manufacturing a composition for treating voice impairment caused by vocal fold fibrosis or vocal fold mucosal tissue damage or loss.

In a fourth aspect, the disclosure encompasses a method of treating voice impairment caused by vocal fold fibrosis or vocal fold mucosal tissue damage or loss. The method includes the step of implanting an engineered vocal fold mucosa as described herein into the larynx of a subject having a vocal impairment. As a result of performing this step, the degree of voice impairment is reduced.

In a fifth aspect, the disclosure encompasses a method of making an engineered vocal fold mucosa. The method includes the steps of (a) culturing a plurality of human vocal fold fibroblasts (VFFs) within a scaffold comprising polymerized collagen; and (b) culturing a plurality of human vocal fold epithelial cells (VFEs) on the scaffold surface. As a result of performing these steps, an engineered vocal fold mucosa is formed that includes an engineered stratified squamous epithelium in contact with an engineered non-vascularized lamina propria made of a polymerized collagen scaffold populated by a plurality of human VFFs within the scaffold.

In some embodiments, the polymerized collagen is polymerized collagen, type I.

In some embodiments, the step of culturing the plurality of human VFEs on the scaffold surface is first performed within an epithelial cell-oriented medium in which the human VFEs are immersed. In some such embodiments, the human VFEs are cultured for 1 to 3 days within the epithelial cell-oriented medium in which the human VFEs are immersed.

By “epithelial cell-oriented medium,” we mean any medium known to or reasonably expected to support the maintenance, growth, and/or proliferation of human epithelial cells in culture, including, without limitation, the epithelial cell-oriented medium disclosed in the Example below.

In some embodiments, the step of culturing the plurality of human VFEs on the scaffold surface is later performed at an air-liquid interface on the scaffold surface, wherein the scaffold comprises a composition comprising both a fibroblast-oriented medium and a epithelial cell-oriented medium. As a result, the human VFEs stratify to form a squamous epithelium layer. In some such embodiments, the VFEs are cultured for 5 to 50 days at the air-liquid interface on the scaffold surface.

In some embodiments, the step of culturing the plurality of human VFFs within the scaffold is first performed when the scaffold includes a fibroblast-oriented medium, and later performed when the scaffold includes a composition comprising both a fibroblast-oriented medium and an epithelial cell-oriented medium.

By “fibroblast-oriented medium,” we mean any medium known to or reasonably expected to support the maintenance, growth, and/or proliferation of human fibroblasts in culture, including, without limitation, the fibroblast-oriented medium disclosed in the Example below.

In some embodiments, prior to culturing the human VFFs within the scaffold, the human VFFs are seeded into the scaffold at a density of between 5×10⁴ and 1×10⁶ VFF/mL. In some such embodiments, the human VFFs are seeded into the scaffold by mixing the VFFs into a collagen composition wherein the collagen has not yet polymerized to form the scaffold. When the collagen subsequently polymerizes into a gel-like composition, the VFFs are cultured within the scaffold.

In some embodiments, the human VFFs are obtained by (a) seeding cells obtained from a human vocal fold mucosa onto an extracellular matrix (ECM)-coated surface, whereby some of the cells adhere to the ECM-coated surface and some of the cells remain free-floating and non-adherent; and (b) culturing the cells that adhere to the ECM-coated surface in fibroblast-oriented medium, resulting in a substantially pure subpopulation of human VFFs. In some such embodiments, the human VFEs are obtained by the further steps of (c) separately culturing the non-adherent cells in an epithelial cell-oriented medium, whereby the non-adherent cells become adherent, and (d) incubating the now-adherent cells in a composition comprising trypsin and removing one or more initially detached cells. In some such embodiments, the remaining adherent cells are detached and passaged into a separate epithelial cell-oriented medium, where the cells again become adherent. In some such embodiments, the passaged cells are again incubated in a composition comprising trypsin, and one or more initially detached cells are again removed, resulting in a substantially pure subpopulation of human VFEs.

In some embodiments, the step of culturing the VFEs, the step of culturing the VFFs, or both further include passaging the cultured cells at least twice. In some such embodiments, the human VFFs, the human VFEs, or both are obtained from the second passage, the third passage, the fourth passage, the fifth passage, the sixth passage, or any combination thereof. In some such embodiments, the human VFFs, the human VFEs, or both are obtained from the third passage.

In some embodiments, the cells are obtained from the human vocal fold mucosa by (a) mincing the human vocal fold mucosa; and (b) enzymatically digesting the minced mucosa to release the cells from the extracellular matrix (ECM).

In a sixth aspect, the disclosure encompasses an engineered vocal fold mucosa as made by the method described herein.

In a seventh aspect, the disclosure encompasses a method of separating cells obtained from human vocal fold mucosa into a substantially pure human VFF subpopulation. The method includes the steps of (a) seeding cells obtained from a human vocal fold mucosa onto an extracellular matrix (ECM)-coated surface, whereby some of the cells adhere to the ECM-coated surface and some of the cells remain free-floating and non-adherent; (b) culturing the cells that adhere to the ECM-coated surface in fibroblast-oriented medium, resulting in a substantially pure subpopulation of human VFFs. In some such embodiments, the cells are further separated into a VFE subpopulation by (c) separately culturing the non-adherent cells in an epithelial cell-oriented medium, whereby the non-adherent cells become adherent, and (d) incubating the now-adherent cells in a composition comprising trypsin and removing one or more initially detached cells. In some such embodiments, the remaining adherent cells are detached and passaged into a separate epithelial cell-oriented medium, where the cells again become adherent. In some such embodiments, the passaged cells are again incubated in a composition comprising trypsin, and one or more initially detached cells are again removed, resulting in a substantially pure subpopulation of human VFEs.

In some embodiments, the cells are obtained from the human vocal fold mucosa by (a) mincing the human vocal fold mucosa; and (b) enzymatically digesting the minced mucosa to release the cells from the extracellular matrix (ECM).

The disclosed compositions and methods are further detailed below.

BRIEF DESCRIPTION OF THE DRAWINGS

The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.

FIGS. 1A-1B demonstrate explant culture of primary human VF mucosal cells. (A) Microscopy images of live cells growing in fibroblast-oriented medium, 14- and 21-days (d) post-seeding. Cells exhibited morphological variance at 14 d but appeared uniform at 21 d. White arrows indicate fibroblast-appearing cells with spindle/star-like somata and elongated processes; white arrowheads indicate epithelial-appearing cells with cuboidal somata and short processes. (B) Microscopy images of live cells growing in epithelial cell-oriented medium, 28 and 35 d post-seeding. Cells exhibited morphological variance with clear fibroblast contamination at 28 d. Non-epithelial cell growth continued at 35 d, leading to mixed-morphology cell sphere formation and difficulty with reattachment during culture passage. White arrows indicate fibroblast-appearing cells with spindle/star-like somata and elongated processes; white arrowheads indicate epithelial-appearing cells with cuboidal somata and short processes. Scale bar, 40 μm (A, B).

FIGS. 2A-2E demonstrate isolation, purification and expansion of primary VFF and VFE from human VF mucosa. (A) Schematic showing general procedure for cell isolation and purification. (B) Light microscopy images showing morphological characteristics of primary VFF and VFE in monolayer culture prior to first passage (left panels; unstained live cells) and at passage 3 (P3; center and right panels; fixed, H&E-stained cells). Black arrows indicate large VFF and VFE. Black arrowheads indicate VFE clusters. Scale bar, 30 μm. (C) Flow cytometry histograms and bar charts showing relative expression of the markers P4H-β, CD90, pan-keratin, keratin 14, keratin 19 and CD227 in VFF and VFE. Positive/negative gates (versus fluorescence-minus-one [FMO] control) are shown in gray; low/high gates are shown in black. (D) Flow cytometry dot plot showing complete separation of VFF and VFE populations via CD90 CD227 double staining. (E) Line graph summarizing VFF and VFE population doubling times from P1 to P6. *, P<0.01 (C, E); n.s., non-significant difference (C); error bars, s.e.m. (C, E).

FIGS. 3A-3G demonstrate assembly of engineered human VF mucosa. (A) Schematic illustrating the experimental approach. (B) H&E- and Movat's pentachrome-stained sections showing comparable morphologic features in engineered and native mucosae. Black arrows indicate basal VFE cytoplasmic projections extending into the lamina propria. Scale bar, 100 μm (main images); 40 μm (insets). (C) Immunofluorescent images showing P4H-β, collagen, type IV, and e-cadherin staining patterns in engineered mucosa compared to native mucosa. White arrows indicate P4H-β⁺ VFE, collagen, type IV⁺ basement membrane and luminal epithelial structures, and e-cadherin⁺ VFE. White arrowheads indicate P4H-β⁺ VFF and collagen, type IV⁺ VFF and vascular basement membrane structures in the lamina propria. Scale bar, 50 μm. (D) Venn diagram summarizing proteome coverage in engineered mucosa compared to native mucosa and scaffold only. FDR, false discovery rate. (E) Functional enrichment analysis of the engineered mucosa proteome. Enriched gene ontology terms are depicted as nodes connected by arrows that represent hierarchies and relationships between terms. Node size is proportional to the number of proteins assigned to a given term; node color represents the Benjamin Hochberg-corrected P-value corresponding to enrichment of the term. Functionally related ontology terms are labeled and grouped using colored ovals (biological process terms in green; molecular function terms in red; cellular component terms in blue). Organogenesis/morphogenesis and ECM functional groups are enlarged for better visualization of individual terms. (F) Heatmaps summarizing normalized spectral abundance factor-based quantification of proteins associated with the ontology terms highlighted in (E). A corresponding list of proteins and fold changes is presented in Table 3. (G) Rheologic data showing elastic (G′) and viscous (G″) moduli of engineered mucosa compared to native mucosa and scaffold only. *, P<0.01; n.s., non-significant difference; error bars, s.e.m.

FIGS. 4A-4E demonstrate VFF distribution and contractile function in collagen, type I scaffold. (A) H&E-stained sections showing VFF density in engineered lamina propria (14 day culture of 2×10⁵ VFF·mL⁻¹ collagen, type I) compared to native human VF lamina propria. Whereas cell density was relatively uniform throughout the engineered lamina propria, it varied considerably throughout the native lamina propria, in part due to the presence of high-cell-density vascular structures (black dashed lines). Cell density in the engineered lamina propria was most similar to the medium-cell-density, non-vascular region of the native lamina propria. Scale bar, 40 μm. (B) Bar graph showing overall cell density in engineered and native lamina propria. *, P<0.01; error bars, s.e.m. (C) Representative schematics and photographs illustrating progressive scaffold contraction following seeding of 2×10⁵ VFF·mL⁻¹ collagen, type I. Scaffold contraction reached a plateau of ˜70% of initial cross-sectional area at 96 hours (h) post-seeding; acellular scaffolds exhibited no contraction. (D) Bar graph showing matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) production during 96 h culture of 2×10⁵ VFF·mL⁻¹ collagen, type I. MMP/TIMP concentrations in culture supernatant reflect accumulation during the 24 h period from 24-48 h post-seeding, and again during the 24 h period from 72-96 h post-seeding. Mean values represent pooled samples from 6 independent biological replicates, assayed using a multiplexed protein array. Medium only was used as a negative control. (E) Immunofluorescent images showing VFF expression of the contractile protein α-actin 2 (ACTA2) in the engineered lamina propria at 48- and 96-hours post-seeding. White arrows indicate representative ACTA2+ VFF. Scaffold only is shown as a negative control. Scale bar, 100 μm (main image); 35 μm (inset).

FIGS. 5A-5B present additional histologic characterization of engineered and native human VF lamina propria. (A) Alcian blue-stained sections showing glycosaminoglycan (GAG) distribution (blue) within the extracellular matrix (ECM). Hyaluronic acid (HA) abundance is indicated by the difference in signal intensity between adjacent (5 μm) sections treated with (right panels) and without (left panels) hyaluronidase. GAG and HA abundance were comparable in the engineered and native lamina propria. (B) Elastic van Gieson-stained sections showing elastin (black) and collagen (pink) fiber distribution within the ECM. The engineered ECM contained thin and sparsely distributed fibrous proteins and appeared immature compared to the native ECM. Blue arrows indicate elastin deposits/fibers; blue arrowheads indicate collagen deposits/fibers. Scale bar, 60 μm (A, B).

FIGS. 6A-6F present proteomic-based analysis of engineered VF mucosa compared to its isolated subcomponents. (A) Schematic illustrating experimental conditions. (B) Venn diagram summarizing proteome coverage and overlap in protein identifications across experimental conditions. FDR, false discovery rate. (C) Volcano plot summarizing normalized spectral abundance factor-based protein quantification in engineered mucosa versus VFF in scaffold (red data points) and VFE on scaffold (blue data points). The dashed rectangle denotes cutoff criteria for protein overrepresentation in engineered mucosa compared to the other conditions (fold change >4; Benjamini Hochberg-adjusted P<0.01). (D) Summary of enriched biological process ontology terms associated with the protein set exclusive to engineered mucosa or overrepresented in engineered mucosa compared to both VFF in scaffold and VFE on scaffold. The table lists the three most highly represented terms identified using the BiNGO enrichment and REViGO term redundancy algorithms, as well as the mechanistically relevant epidermis development term (a complete list of enriched biological process terms is presented in Table 5). The heatmap shows the relative abundance of overrepresented proteins that map to these biological process terms of interest. Pleiotropic proteins associated with multiple terms are assigned multiple rows in the heatmap. The enlarged region highlights 5 proteins associated with epidermis (in the context of mucosa, epithelium) development. (E) Immunohistochemical validation of overrepresented proteins LAMAS (costained with COL4), KRT5, and JUP (costained with CDH1) in engineered and native VFmucosae. White arrows indicate KRT5+ VFE; white arrowheads indicate COL4+ signals in the deep epithelium and JUP+ VFE; and yellow arrows indicate LAMA5+COL4+ basal VFE in engineered mucosa, LAMA5+COL4+ basement membrane structures in native mucosa, and CDH1+JUP+ VFE. Scale bar, 50 mm; 25 mm (inset). (F) Transmucosal electrical resistance data showing significantly increased physiologic barrier function in engineered mucosa compared to scaffold only and VFF in scaffold, and no significant difference compared to VFE on scaffold. *, P<0.01; n.s., non-significant difference; error bars, s.e.m.

FIGS. 7A-7B present additional quantitative proteomic analysis of engineered VF mucosa compared to its isolated subcomponents. (A) Volcano plot and heatmap showing normalized spectral abundance factor-based protein quantification in engineered mucosa versus VFF in scaffold. The cyan dashed rectangle (Volcano plot) and square bracket (heatmap) denote cutoff criteria for protein overrepresentation in engineered mucosa compared to VFF in scaffold (fold change >4; Benjamini Hochberg-adjusted P<0.01); the magenta dashed rectangle (Volcano plot) and square bracket (heatmap) denote cutoff criteria for protein overrepresentation in VFF in scaffold compared to engineered mucosa (fold change <−4; Benjamini Hochberg-adjusted P<0.01). (B) Parallel analysis of engineered mucosa versus VFE on scaffold. The cyan dashed rectangle (Volcano plot) and square bracket (heatmap) denote cutoff criteria for protein overrepresentation in engineered mucosa compared to VFE on scaffold (fold change >4; Benjamini Hochberg-adjusted P<0.01); the magenta dashed rectangle (Volcano plot) and square bracket (heatmap) denote cutoff criteria for protein overrepresentation in VFE on scaffold compared to engineered mucosa (fold change <−4; Benjamini Hochberg-adjusted P<0.01). A complete list of overrepresented proteins with corresponding fold changes and P-values is presented in Table 4.

FIGS. 8A-8H demonstrate ex vivo physiologic performance of engineered VF mucosa in a large animal excised larynx setup. (A) Sequential photographs of a canine larynx under native conditions (upper panel), following unilateral VF mucosa resection (center panel), and following unilateral placement of engineered VF mucosa (lower panel). (B) Schematic illustrating the experimental conditions shown in (A) in coronal orientation in the excised larynx setup. HSDI, high-speed digital imaging; TA, thyroarytenoid. (C) Aerodynamic data showing phonation threshold pressure (P_th), subglottal pressure (P_s) and flow (U) relationships (i.e., glottal resistance [R_g] plots), aerodynamic input power (P_aero) and radiated acoustic output power (P_ae) relationships (i.e., glottal efficiency [E_g] plots). VF mucosa resection eliminated vibratory capacity below P_s=5 kPa (preventing R_g and E_g measurement under this condition), whereas the engineered mucosa restored function with comparable R_g and E_g to native mucosa. (D) HSDI-based glottal area analysis showing restoration of waveform morphology and area magnitude with placement of engineered VF mucosa. The upper panels represent sequential resection and engineered mucosa replacement within a single larynx. Grey arrows indicate the beginning, midpoint and endpoint of a single 5.8-ms-duration vibratory cycle. The yellow dashed ellipse indicates maximum glottal area. The yellow horizontal dashed line indicates the midmembranous plane used for subsequent kymographic analyses in (E, F). Scale bar, 3 mm. (E) Representative kymograms from the larynx presented in (D) showing restoration of typical vibratory physiology (lateral [left-right] phase similarity, vertical phase difference, mucosal wave excursion) following engineered VF mucosa replacement. Sinusoidal curve fitting (R²>0.98) to the upper and lower VF margins (UM; LM) is shown for the native and engineered conditions. Red dashed lines indicate open and closed phases of a single vibratory cycle. Yellow dashed lines indicate UM and LM·f₀, fundamental frequency. (F) Analysis of lateral and vertical phase differences for all larynges and conditions, showing comparable vibratory physiology for native and engineered VF mucosae. #, contralateral VF mucosa condition used to calculate lateral phase difference; !, VF mucosa condition contralateral to that for which vertical phase difference is calculated. (G) Representative acoustic data showing time-domain signals (upper panels), narrowband spectrograms (center panels) and phase plots (lower panels). VF resection resulted in signal aperiodicity, loss of harmonic structure and deterministic chaos; the engineered mucosa restored signal periodicity and measurable f₀, harmonic structure and a closed phase trajectory. (H) Summary of qualitative acoustic signal typing for all larynges and conditions. Native and engineered VF mucosae generated near-periodic type 1 signals, whereas mucosal resection resulted in predominantly stochastic type 4 signals. Data from the same larynx are plotted in the same color (C, D, F, H); *, P<0.01 (C, D, H); n.s., non-significant difference (C, D, F, H). Data from a parallel experiment evaluating the ex vivo physiologic performance of human oral mucosa are presented in FIGS. 10A-10G.

FIGS. 9A-9G demonstrate immunogenicity of engineered VF mucosa. (A) Flow cytometry histograms and bar charts showing expression of cell surface markers HLA-ABC, HLA-DR, CD80, CD86, PD-L1 (CD274) and PD-L2 (CD273) in VFF and VFE compared to peripheral blood mononuclear cell (PBMC) control. Gates are based on isotype controls. (B) Schematic illustrating the experimental approach used to evaluate in vivo immunogenicity via subrenal capsule auto/allograft transplantation in the humanized NOD-scid IL2r^γnull (NSG) mouse model. GVHD, graft-versus-host disease; hPBL, human peripheral blood lymphocyte. (C) Line graph showing decrease in body mass of NSG mice following hPBL injection, compared to no hPBL control mice. Mice were euthanized in response to a >15% decrease in body mass and clinical signs of xenogeneic GVHD, which occurred 15-21 d post-hPBL injection. (D) Flow cytometry dot plots and bar graph confirming engraftment and proliferation of hCD⁴⁵⁺mCD45⁻ human lymphocytes in the peripheral blood of NSG mice following hPBL injection. (E) Immunostained sections showing predominant hCD4⁺ T helper cell infiltration and minimal hCD8⁺ cytotoxic T cell infiltration of the engineered grafts, 15 d post-hPBL engraftment. Comparable staining patterns were observed in the auto- and allografts. Dashed black contour lines indicate the boundaries between the engineered human grafts (top) and the mouse kidneys (bottom). Scale bar, 500 μm. (F) Analysis of hFOXP3 expression by infiltrating hCD4⁺ T cells, 15 d post-hPBL engraftment. The immunofluorescent image shows representative hCD4 and hFOXP3 expression in the engineered allograft; the bar graph summarizes cell count data showing a higher percentage of hCD4⁺hFOXP3⁺ regulatory T cells in the engineered auto- and allografts compared to the mouse eyelid, a GVHD-affected positive control tissue. White arrows indicate hCD4⁺hFOXP3⁻ T helper cells; yellow arrows indicate hCD4⁺hFOXP3⁺ regulatory T cells; the white arrowhead indicates a hCD4⁻hFOXP3⁻ cell. Scale bar, 5 μm. (G) H&E-stained sections showing intact tissue morphology in the engineered auto- and allografts, compared to extensive cellular infiltration and destruction of naïve tissue morphology in the mouse eyelid at 15 d post-hPBL engraftment. Scale bar, 50 μm. *, P<0.01 (A, C, D, F); n.s., non-significant difference (A, F); error bars, s.e.m. (A, D, F).

FIGS. 10A-10G demonstrate ex vivo physiologic performance of human oral mucosa, compared to that of engineered VF mucosa, in a large animal excised larynx setup. (A) Coronal orientation schematic illustrating unilateral placement of oral or engineered VF mucosa following resection of native VF mucosa. HSDI, high-speed digital imaging; TA, thyroarytenoid. (B) Phonation threshold pressure (P_th) data showing elevated threshold in the oral mucosa condition compared to the engineered VF mucosa condition. (C) HSDI-based glottal area analysis showing normal-appearing waveform morphology but reduced area magnitude in the oral mucosa condition compared to the engineered VF mucosa condition. P_s, subglottal pressure. (D) Representative kymogram from the larynx presented in (C) showing grossly intact lateral (left-right) phase symmetry but reduced vibratory amplitude and mucosal wave excursion following oral mucosa placement, compared to contralateral native VF mucosa. Sinusoidal curve fitting (R²>0.98) to the upper and lower VF margins (UM; LM) is also shown. Red dashed lines indicate open and closed phases of a single vibratory cycle. Yellow dashed lines indicate UM and LM·f₀, fundamental frequency. (E) Analysis of lateral and vertical phase differences for all larynges in the oral and engineered VF mucosa conditions. No significant differences were observed. #, contralateral VF mucosa condition used to calculate lateral phase difference; !, VF mucosa condition contralateral to that for which vertical phase difference is calculated. (F) Acoustic data showing a representative time-domain signal (upper panel), narrowband spectrogram (center panel) and phase plot (lower panel) obtained from a larynx in the oral mucosa condition. Placement of oral mucosa resulted in a periodic signal with measurable f₀, some harmonic structure, and a closed phase trajectory. (G) Summary of qualitative acoustic signal typing for the oral and engineered VF mucosa conditions. Both mucosae generated near-periodic type 1 signals in the majority of experimental runs. The engineered VF mucosa data used for statistical comparisons (B, C, E, G) are presented in complete form in FIGS. 8A-H; *, P<0.01 (B, C); n.s., non-significant difference (E, G).

FIGS. 11A-11C present additional in vivo graft survival and immunogenicity data. (A) Immunostained sections showing long-term survival of engineered VF mucosa following subrenal capsule transplantation in the NOD-scid IL2rγ^null (NSG) mouse. Black arrows indicate positive intracellular immunostaining of human mitochrondia, 78 days (d) following graft placement with no human peripheral blood lymphocyte (hPBL) injection. Scale bar, 30 μm. (B) Immunostained section showing long-term survival of the collagen, type I scaffold, 78 days following graft implantation with no hPBL injection. No hCD45 immunosignal was observed, consistent with the absence of human cells in both the implant and the NSG mouse recipient. Scale bar, 100 μm. (C) Immunostained section showing performance of the collagen, type I scaffold in the NSG mouse, 21 d following hPBL injection. Black arrows indicate hCD45⁺lymphocytes at the boundary of the scaffold. No lymphocyte infiltration of the scaffold was observed. Separation of the scaffold and kidney represents artifact introduced by tissue processing and sectioning. Scale bar, 100 μm. The dashed black contour lines indicate the boundaries between the engineered human graft/collagen, type I scaffolds (top) and the mouse kidneys (bottom) (A, B, C).

DETAILED DESCRIPTION

This application discloses engineered vocal fold mucosae made from isolated and purified human vocal fold fibroblasts and isolated and purified human VF epithelial cells, co-cultured under organotypic conditions. When grafted into larynges ex vivo, the engineered vocal fold mucosae generate physiologically appropriate vibratory behavior and acoustic output that are indistinguishable from those of native VF tissue, thus demonstrating that they have the biomechanical properties that are essential for use as an implant in treating voice impairment due to vocal fold mucosal tissue damage, loss, or disease. In addition, when grafted into humanized mice in vivo (a transgenic mouse model supporting a functional human adaptive immune system; see Schulz, L. D., et al., Nat. Rev Immunol 12, 786-798 (2012)), the mucosae survive and are well tolerated by the human adaptive immune system, indicating that implanting the engineered mucosa into the larynx of a patient would likely not trigger rejection or immune system attack against the implant.

The inventors have found that using human vocal fold mucosal cells in the engineered vocal fold mucosa imparts the unique biomechanical and immunological properties that make the engineered mucosa useful as an implant for restoring vocal function in humans. Without being bound by any theory, human-sourced vocal fold mucosal cells are derived from cells that have been exposed to unique mechanical forces and vibration during voice production, and are thus specifically adapted to have the functional and underlying structural characteristics necessary to support phonation.

Regarding the human immunotolerance engendered by the grafted engineered vocal fold mucosa, the vocal fold mucosal cells may have immunoprivilege conferred by the unique position in the body of the cells from which they are derived. Specifically, humans are constantly inhaling a variety of potentially immunogenic foreign matter, much of which would come in contact with the vocal fold mucosae. If the vocal fold mucosal cells were immunologically active, the vocal fold mucosa would be continuously inflamed, resulting in chronic laryngitis and other airway problems. Thus, vocal fold mucosal cells may have evolved to favor and promote immunotolerance, as exhibited by the engineered vocal fold mucosa.

The engineered vocal fold mucosa is capable of exhibiting the vibratory function and acoustic output of a native vocal fold mucosa. Such capability can be measured using any of a number of specific tests known in the art, including, without limitation, the physiological, vibratory, and acoustic tests using ex vivo canine larynges that are described in detail in the Example below.

In the disclosed method of making the engineered vocal fold mucosa, the human VFFs may be cultured in a fibroblast-oriented medium or in a composition containing a fibroblast-oriented medium. The human VFEs may be cultured in a epithelial cell-oriented medium or in a composition containing an epithelial cell-oriented medium. By “fibroblast-oriented medium,” we mean any medium known to or reasonably expected to support the maintenance, growth, and/or proliferation of human fibroblasts in culture, including, without limitation, the fibroblast-oriented medium disclosed in the Example below. By “epithelial cell-oriented medium,” we mean any medium known to or reasonably expected to support the maintenance, growth, and/or proliferation of human airway epithelial cells in culture, including, without limitation, the epithelial cell-oriented medium disclosed in the Example below.

The human vocal fold mucosa can be useful for various in vitro and in vivo applications. Preparations for use in clinical applications must be obtained in accordance with regulations imposed by governmental agencies such as the U.S. Food and Drug Administration. Accordingly, in exemplary embodiments, the methods provided herein are conducted in accordance with Good Manufacturing Practices (GMPs), Good Tissue Practices (GTPs), and Good Laboratory Practices (GLPs). Reagents comprising animal derived components are not used, and all reagents are purchased from sources that are GMP-compliant. In the context of clinical manufacturing of a bioengineered implant for use in restoring vocal function in humans, GTPs govern cell donor consent, traceability, and infectious disease screening, whereas GMPs are relevant to the facility, processes, testing, and practices to produce consistently safe and effective products for human use. See Lu et al. Stem Cells 27: 2126-2135 (2009). Where appropriate, oversight of patient protocols by agencies and institutional panels is envisioned to ensure that informed consent is obtained; safety, bioactivity, appropriate dosage, and efficacy of products are studied in phases; results are statistically significant; and ethical guidelines are followed.

As used herein, the term “engineered tissue” (or similar term) refers to a tissue prepared in accordance with the methods of the invention. An acceptably engineered tissue displays physical characteristics typical of the type of the tissue in vivo and functional characteristics typical of the type of the tissue in vivo, i.e., has a functional activity. For example, physical characteristics of an engineered vocal fold mucosa can include the presence of a lamina propria layer and a squamous epithelium layer. Functional characteristics of an engineered vocal fold mucosa can include the capacity of the engineered tissue for exhibiting the vibratory function and acoustic output of a native vocal fold mucosa. As used herein, the term “bioengineered” generally refers to a tissue prepared in vitro using biological techniques including, for example, techniques of cell biology, biochemistry, tissue culture, and materials science.

The following abbreviations and acronyms are used in this application: VF, vocal fold; VFF, vocal fold fibroblasts; VFE, vocal fold epithelial cells; ECM, extracellular matrix.

Each of the publications cited in this application is incorporated by reference in its entirety and for all purposes. While specific embodiments and examples of the disclosed subject matter have been discussed herein, these examples are illustrative and not restrictive. Many variations will become apparent to those skilled in the art upon review of this specification and the claims below.

The following Example is offered for illustrative purposes only, and is not intended to limit the scope of the present invention in any way. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description and the following example and fall within the scope of the appended claims.

Example

Bioengineered Vocal Fold Mucosa for Voice Restoration

In this Example, we generated bioengineered vocal fold mucosae from isolated and purified vocal fold fibroblasts (VFF) and epithelial cells (VFE). Specifically, we initially hypothesized that primary human-sourced VF mucosal cells, exposed to unique mechanical forces during human voice production^20,21, could be an appropriate cell source for the development of a bioengineered VF mucosa capable of recapitulating native VF physiologic function. We therefore isolated and purified primary vocal fold fibroblasts (VFF) and epithelial cells (VFE) from individual human donors and cultured these cells under 3D organotypic conditions, based on techniques commonly employed in skin and other mucosal systems^22,23. The resulting engineered mucosae showed morphologic resemblance to native human VF mucosa, proteome-level evidence of active organogenesis/morphogenesis along with emerging ECM complexity, and rudimentary epithelial barrier function in vitro. Using a large animal ex vivo setup, we observed aerodynamic-to-acoustic energy transfer, periodic VF vibratory motion with physiologic mucosal wave travel, and acoustic output that were each indistinguishable from those generated by native tissue. Using a humanized mouse system, we implanted engineered VF mucosal auto- and allografts in vivo and documented robust graft survival with favorable tolerance by the human adaptive immune system. Taken together, these results indicate that the disclosed bioengineered mucosae have the potential to restore voice function in patients with otherwise untreatable VF mucosal disease.

Results

Isolation and Characterization of Primary Cells from Human VF Mucosa.

Primary VF mucosal cell culture is rarely feasible with disease-free tissue from human donors, as elective biopsy carries an unacceptable risk of lamina propria scar formation and dysphonia. For this reason, and because of associated technical challenges, there are no published reports of isolation, purification and primary culture of VFF and VFE from a single human donor. We obtained human tissue from cadavers at autopsy (<6 h post-mortem) and patients undergoing total laryngectomy for indications that did not include laryngeal disease (e.g., hypopharyngeal cancer, dysphagia with otherwise untreatable pulmonary aspiration) and then initially conducted primary explant culture in fibroblast- or epithelial cell-oriented medium. Fibroblast-oriented culture resulted in steady VFF proliferation and successful deletion of non-target cells, yielding a morphologically pure VFF population within 21 d (FIG. 1A); however, epithelial cell-oriented culture resulted in limited VFE proliferation alongside growth of non-epithelial cells, including those with fibroblastic morphology. We observed tangling of various cell subpopulations within the culture dish (FIG. 1B) and heterogeneous cell sphere formation leading to problems with reattachment during culture passage.

To address these issues, we developed an approach to better isolate and purify VFF and VFE from human VF mucosa (FIG. 2A). We microdissected and minced the mucosa; performed enzymatic digestion to release cells from the ECM; filtered, washed and pipetted cell clumps to release individual cells; separated the cells into VFF and VFE subpopulations based on their adhesion capacity on ECM-coated culture surfaces (see Materials and Methods below); and cultured under fibroblast- or epithelial cell-oriented conditions. These VFF and VFE proliferated well in primary culture and formed colonies containing generally homogenous cell morphology (FIG. 2B). VFF formed initial large colonies 10-15 d post-seeding: the majority of cells contained classic-appearing spindle/star-like somata and elongated processes. VFE formed initial colonies 20-25 d post-seeding: the majority of cells contained relatively large nuclei, cuboidal somata, and short processes. We observed a persistent subpopulation of morphologically similar but notably large cells in both VFF and VFE cultures.

Fibroblasts and epithelial cells exhibit distinct immunomarkers in vivo but share expression of most markers (at different abundances) in vitro.^24,25 We therefore used a 6-marker flow cytometry panel to characterize the relative expression of classic fibroblast- and epithelial cell-associated proteins in our adhesion-separated VFF and VFE (FIG. 2C). Relative expression levels (based on low/high gating) were consistent with cell phenotype; however, as expected, single marker analysis was ineffective at completely separating the two subpopulations. VFF expressed more of the collagen synthesis enzyme prolyl-4-hydroxylase β (P4H-β) and fibroblast marker CD90 (also known as Thy-1) (P<0.01). VFF and VFE expressed equivalent pan-keratin (P=0.73), whereas VFE expressed more of the type I keratin isoforms 14 and 19, as well as the mucin-producing epithelial cell marker CD227 (also known as epithelial membrane antigen or mucin 1) (P<0.01). Subsequent double staining resulted in successful separation of these VF mucosal cells into CD90^hiCD227^lo (VFF) and CD90^loCD227^hi (VFE) subpopulations (FIG. 2D), confirming the effectiveness of our isolation and purification workflow.

VFF maintained a consistent proliferation rate (30-40 h population doubling time) over 6 passages (FIG. 2E). VFE proliferated more slowly overall (P<0.01), initially maintaining a 55-65 h population doubling time that progressively increased across passages 4-6. We therefore used passage 3 cells for subsequent mucosal engineering experiments.

Assembly of 3D Engineered VF Mucosa.

Having successfully identified a viable source of purified human primary cells, we pursued 3D organotypic culture (FIG. 3A) in polymerized collagen, type I, a primary ECM constituent of native human VF mucosa²⁶. Initial trials using 2×10⁵ VFF·mL⁻¹, without VFE, resulted in a final intrascaffold cell density comparable to the medium-cell-density, non-vascular region of the native lamina propria (FIG. 4a) and ˜50% of mean cell density across the entire (vascularized) native lamina propria (P<0.01; FIG. 4B). We further observed moderate scaffold contraction over the first 96 h of culture (FIG. 4C), which corresponded to expression of a subset of matrix metalloproteinase (MMP) and tissue inhibitor of mellatoproteinase (TIMP) enzymes/inhibitors (FIG. 4D), as well as the contractile protein α-actin 2 (also known as α-smooth muscle actin) (FIG. 4E). We maintained this 2×10⁵ VFF·mL⁻¹ seeding density in subsequent experiments, followed by VFE seeding at 24 h, media-immersed VFF-VFE coculture for 48 h, and coculture with VFE at the air-liquid interface for a further 8-28 d.

The engineered VF mucosa began to resemble native mucosa after 10-14 d in culture, exhibiting a ˜50 μm-thick stratified squamous epithelium and sparsely cell populated lamina propria (FIG. 3B). Basal VFE processes extended into the underlying lamina propria, consistent with epithelial anchoring. The lamina propria contained glycosaminoglycans (GAGs), including the biomechanically important GAG hyaluronic acid²⁷ (FIG. 5A); however its ECM appeared immature overall, populated by sparsely distributed fibers that did not resemble the sophisticated ECM network of native mucosa (FIG. 5B). P4H-β⁺ VFF were identified throughout the lamina propria, and P4H-β⁺VFE were preferentially localized to the basal epithelium near the basement membrane, as seen in native mucosa (FIG. 3C). Basal VFE also expressed the basement membrane marker collagen, type IV, which formed an emerging barrier-like structure in the subepithelium. Unlike native mucosa, however, a comparable collagen, type IV⁺ structure was also observed at the luminal epithelial surface. The majority of VFE were E-cadherin⁺, suggesting early establishment of intercellular junctional complexes that approximated those seen in native VF mucosa.

To further characterize the biological complexity of the engineered VF mucosa, we conducted discovery proteomic analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Using a 1% false discovery rate, we identified 762 unique proteins in the engineered mucosa, compared with 908 in the native mucosa and 32 in the collagen, type I-based scaffold (FIG. 3D; Table 1). Gene ontology-based enrichment analysis of the engineered VF mucosa proteome, as compared to the full human protein database (Uniprot), revealed a wide complement of functional protein sets (ontology terms) associated with various metabolic, catalytic, transport, binding and signaling processes; spanning an array of subcellular and extracellular locations (FIG. 3E; Table 2). We identified organogenesis/morphogenesis-specific ontology term enrichment, consistent with successful organotypic culture; as well as ECM terms indicative of protein complexes and anatomic substructures that are characteristic of native VF mucosa, such as the basal lamina, anchoring collagen and fibrillar collagen. Follow-up normalized spectral abundance factor (NSAF)-based quantitative analysis²⁸ showed strong correspondence between the engineered and native mucosae (FIG. 3F; Table 3), implying that most of the ECM subproteome in the engineered mucosa is attributable to new protein synthesis by VFF and VFE, rather than the original scaffold. We identified ECM proteins and glycoconjugates that are considered critical to the biomechanical capacity of native VF mucosa for self-sustained physiologic vibration^26,29-32, including: multiple collagen isoforms; the elastin conduit fibrillin 1 and elastin microfibril interface-located protein (EMILIN) 1; the small leucine-rich repeat proteoglycans decorin, lumican and biglycan; and the glycoproteins fibronectin, fibulin 1 and tenascin X. These observations suggest that while the engineered lamina propria appeared immature on histologic analysis, its developing ECM is populated by a core set of protein constituents with the potential to support vibratory function. This conclusion was further supported by rheologic experiments showing that the viscoelastic profile of the engineered mucosa was more similar to that of the native mucosa (P=0.51 and 0.88 for elastic [G′] and viscous [G″] moduli, respectively; FIG. 3G) than that of the scaffold (P<0.01 for both G′ and G″).

Quantitative Proteomic Analysis of Engineered VF Mucosa Compared to its Isolated Subcomponents.

We pursued further quantitative proteomic analyses to identify protein complexes and functionality that were unique to the engineered VF mucosa, compared to its isolated subcomponents: VFF in collagen, type I scaffold and VFE on collagen, type I scaffold (FIG. 6A). Proteome coverage and overlap were generally comparable, with 528 proteins common to all three conditions and 59 proteins unique to the engineered mucosa (FIG. 6B; Table 1). NSAF-based analysis showed that the majority of differentially abundant proteins were overrepresented in engineered VF mucosa, as compared to the isolated subcomponents (fold change >4; adjusted P<0.01; FIG. 6C), suggesting upregulation of a specific protein set due to VFF-VFE synergy in organotypic culture. Subsequent pairwise comparisons indicated that the engineered mucosa proteome was most similar to VFE on scaffold and least similar to VFF in scaffold (69 versus 139 differentially abundant proteins, respectively; FIG. 7; Table 4), consistent with VFE being the predominant cell type in the engineered mucosa.

Next, we performed functional enrichment analysis of the protein set that was either exclusively identified or quantitatively overrepresented in engineered VF mucosa compared to both VFF in scaffold and VFE on scaffold. The most highly represented biological process ontology terms (adjusted P<4×10⁻⁵) indicated that the engineered mucosa was uniquely engaged in macromolecule catabolism, protein localization, and cellular component organization or biogenesis (FIG. 6D), suggesting dynamic structural assembly. Further evaluation of the deep ontology output revealed significant enrichment of additional terms relevant to mucosal assembly: cell-substrate junction assembly (adjusted P=3×10⁻³), epidermis development (adjusted P=5.3×10⁻³), adherens junction organization (adjusted P=7.4×10⁻³) and cell junction assembly (adjusted P=8.7×10⁻³) (Table 5). Enrichment of these ontology terms was driven by a common protein set that was significantly overrepresented in engineered VF mucosa compared to the other experimental conditions, consisting of the basal lamina constituent laminin 5, the basal epithelial cell marker keratin 5, the desmosome constituents junction plakoglobin (also known as γ-catenin) and periplakin, as well as collagen, type V.

Emergence of Immature Barrier Function in Engineered VF Mucosa.

As our histologic and proteomic data suggested the emergence of basement membrane structures and epithelial junctional complexes under organotypic culture conditions, we performed follow-up immunovalidation of laminin 5, keratin 5 and junction plakoglobin expression in engineered VF mucosa, and compared the distribution of these proteins to native VF mucosa. Immunohistochemistry confirmed that each of these targets was expressed in the engineered VF epithelium but lacked the region-specific localization of native mucosa (FIG. 6E). Laminin 5 was expressed by basal and suprabasal VFE and showed intracellular colocalization with its functional partner collagen type IV, but these proteins did not form mature basal laminae as seen in native tissue. Keratin 5⁺ VFE were scattered throughout the engineered epithelium but were not preferentially localized to the basal region. Junction plakoglobin was expressed by the majority of VFE and generally colocalized with its binding partner e-cadherin, but these proteins did not exhibit the intercellular distribution pattern of mature junctional complexes within native VF epithelium.

To further evaluate physiologic barrier function in the engineered mucosa, we conducted transmucosal electrical resistance experiments. These experiments required reducing fibroblast seeding density by ˜3.5-fold (to 5.6×10⁴ VFF·mL⁻¹) to reduce scaffold contraction and eliminate detachment from the insert wall. Under comparable culture conditions, the engineered mucosa had significantly greater resistance than scaffold only and VFF in scaffold (P<0.01), but showed no significant difference compared to VFE on scaffold (P=0.06; FIG. 6F), corroborating our immunohistochemical findings and confirming that while emerging basement membrane and epithelial junctional complexes were present, physiologic barrier function remained immature.

Ex Vivo Physiologic Function of Engineered VF Mucosa.

Given the favorable morphologic and histologic appearance of engineered VF mucosa compared to native tissue, we scaled-up our engineering approach to create human-sized tissues, and evaluated their physiologic performance in a large animal excised larynx setup. This ex vivo approach holds key methodological advantages over in vivo techniques for studying VF physiology, due to greater control of anatomic, postural and aerodynamic input parameters, direct visual exposure of the VFs during vibration, and more precise multichannel measurement. We used canine larynges based on their anatomic similarity to human specimens and precedence in excised larynx studies^33-35 and collected sequential datasets from each larynx: (i) with bilateral native VFs intact, (ii) following unilateral VF mucosa resection to impair physiologic function, and (iii) following unilateral placement of engineered VF mucosa in an attempt to restore function (FIG. 8A).

We collected a complete array of aerodynamic, high-speed digital imaging (HSDI) and acoustic data (FIG. 8B). Following arytenoid adduction, we delivered humidified air at gradually increasing subglottal pressures (P_s) to initiate flow (U)-induced VF vibration. Phonation threshold pressure (P_th), the minimum P_s required to initiate vibration, was ˜1 kPa in the native condition, but was eliminated at P_s up to 5 kPa following VF mucosa resection (FIG. 8C). Placement of engineered mucosa restored vibration with comparable P_th to the native condition (P=0.80). Native and engineered mucosae exhibited similar within-larynx glottal resistance (R_g), aerodynamic input power (P_aero), radiated acoustic power (P_ac), and glottal efficiency (E_g) (FIG. 8C). P_aero to P_ac loss resulted in E_g values of ˜10⁻²-10⁻⁴% across all runs, consistent with previously reported data from excised larynx experiments in the absence of a vocal tract³⁴.

HSDI analysis showed restoration of typical vibratory physiology following engineered VF mucosa replacement. Glottal area waveforms and displacement values were similar to those generated by native mucosa, particularly for within-larynx comparisons (P=0.26; FIG. 8D). Engineered mucosa vibrated with a clear vertical phase difference between upper and lower margins (UM; LM), intact mucosal wave excursion, and lateral (left-right) phase symmetry with the contralateral VF (FIG. 8E). Using HSDI-extracted kymograms, we fitted sinusoidal curves to the UM and LM of each VF (R²>0.98) and observed comparable phase differences for all pairwise comparisons within the experiment (P=0.09-0.76 for all comparisons; FIG. 8f). Acoustic analysis confirmed these vibratory physiology findings, showing restoration of signal periodicity, harmonic structure, and closed phase trajectories following placement of engineered mucosa (FIG. 8g). Using narrow-band spectrography-based qualitative signal typing^36,37, native and engineered mucosae-generated acoustic signals were uniformly categorized as type 1 (near-periodic), whereas signals associated with mucosal resection were uniformly categorized as type 4 (stochastic) (FIG. 8H). In total, the aerodynamic, vibratory and acoustic performance of the engineered mucosa was functionally equivalent to that of native tissue.

Using the same ex vivo experimental set-up, we next compared the physiologic function of engineered VF mucosa with that of human oral mucosa (FIG. 9A), an autologous free graft material traditionally used for reconstructing large VF mucosal deficits^38,39. Oral mucosa vibrated with elevated P_th (P<0.01; FIG. 9B), as well as reduced glottal area magnitude and mucosal wave excursion (P<0.01; FIGS. 9C, D), suggesting a poor viscosity match to native tissue^20,40. Lateral and vertical phase differences were comparable across oral and engineered VF mucosa conditions (P=0.10-0.61 for all comparisons; FIG. 9E) and the oral mucosa generated type 1 acoustic signals with some harmonic structure in the majority of experimental runs (FIGS. 9F, G). These data indicate that, in addition to having comparable physiologic function to native VF mucosa, the engineered VF mucosa is superior to oral mucosa, a standard-of-care material used in current surgical practice.

Immunogenicity of Engineered VF Mucosa.

To further evaluate its potential for therapeutic implementation, we tested the immunogenicity of engineered VF mucosa and its constituent cells using flow cytometry and an in vivo transplantation assay. Previous work has shown that primary VFF express a cell-surface phenotype that is comparable to immunoprivileged mesenchymal stein cells⁴¹, however no such data have been reported for VFE. VFF and VFE uniformly expressed the pan-major histocompatibility complex (MHC) class I marker human leukocyte antigen (HLA)-ABC (common to all nucleated cells), but were negative for the pan-MHC class 11 marker HLA-DR (associated with professional antigen-presenting cells), as well as the T cell costimulatory molecules CD80 and CD86 (FIG. 10A). Further, 45-75% of VFF and VFE expressed the T cell inhibiting and tolerance-promoting molecules programmed death-ligand (PD-L)1 and PD-L2 (also known as CD274 and CD273, respectively). These findings suggest that naïve VFF and VFE have limited antigen-presentation capacity and may actively promote immunotolerance in a transplantation context.

Given these encouraging in vitro data, we next evaluated in vivo survival and tolerance of engineered VF mucosa following subrenal capsule auto- and allograft transplantation in the humanized NOD-scid IL2rγ^null (NSG) mouse. This transgenic model contains a targeted mutation causing complete silencing of the interleukin-2 receptor γ-chain (IL2rγ) locus, which results in severely restricted B, T, and natural killer (NK) cell development⁴². The NSG model supports engraftment of human peripheral blood lymphocytes (hPBL) and establishment of a functional human adaptive immune system, allowing evaluation of graft immunogenicity during the period prior to terminal xenogeneic graft-versus-host disease (GVHD).

We implanted engineered VF mucosal grafts from unrelated human donors under the bilateral renal capsules of irradiated NSG mice, followed by intravenous delivery of 13×10⁶ hPBL that were obtained from an original laryngeal tissue donor and autologous to one of the two grafts (FIG. 10B). Mice exhibited a significant decrease in body mass (P<0.01; FIG. 10C) and progressive onset of clinical GVHD signs following hPBL injection; flow cytometry analysis confirmed engraftment and subsequent proliferation of hCD45⁺mCD45⁻ human lymphocytes in mouse peripheral blood (FIG. 10D). Evaluation of graft outcomes 15-21 d post-hPBL engraftment showed predominant infiltration by hCD4⁺ T helper cells and minimal infiltration by hCD8⁺ cytotoxic T cells, with no observable difference between the auto- and allograft conditions (FIG. 10E). Follow-up analysis of forkhead box P3 (hFOXP3) coexpression by the infiltrating hCD4⁺ cells revealed a significantly higher subpopulation of hCD4⁺hFOXP3⁺ regulatory T cells in the engineered auto- and allografts compared to the mouse eyelid, a GVHD-affected positive control tissue (P<0.01; FIG. 10F). This finding corresponded to preservation of tissue morphology in the engineered auto- and allografts, which contrasted with extensive cellular infiltration and destruction of naïve tissue morphology in the mouse eyelid (FIG. 10G).

The identification of a robust subpopulation of regulatory T cells in the auto- and allografts, combined with the absence of tissue destruction in both conditions, suggests that engineered VF mucosa holds low immunogenicity and is well tolerated by the human adaptive immune system in vivo. Additional long-term experiments in NSG mice without hPBL challenge confirmed that the grafts survived and remained populated by human cells for the entire 70-98 days (d) experimental time course (FIG. 10C; FIG. 11A). The collagen, type I scaffold exhibited comparable long-term survival (FIG. 11B). When implanted in hPBL-induced humanized NSG mice, the acellular scaffold exhibited no hCD45⁺ lymphocyte infiltration (FIG. 11C) despite active GVHD in the host, indicating absence of recognition by the human adaptive immune system. This observation is consistent with previous reports of minimal host response to xenogeneic collagen in human clinical applications⁴³.

Discussion

We have met a series of important milestones towards the development of a fully functioning bioengineered VF mucosa suitable for therapeutic transplantation. These milestones include: concurrent isolation and purification of primary VFF and VFE from individual human donors; 3D organotypic primary culture resulting in recapitulation of key morphologic features and emerging barrier function; confirmation of size scalability; restoration of normal-appearing physiologic vibratory function and acoustic output; and demonstration of tolerance by the human adaptive immune system. The robust biomechanical performance and low immunogenicity of this engineered VF mucosa indicates that our approach could be used to restore voice function in patients with otherwise untreatable VF mucosal impairment or loss.

Although difficult to obtain, and never previously isolated and purified in parallel, we used primary human VFF and VFE as a source of human VFFs and VFEs based on the rationale that these cells are ideally suited for VF mucosal organotypic culture due to their prolonged exposure to phonation-associated mechanical forces in vivo^20,21. This approach was successful, and under permissive conditions the cells engaged in mucosal morphogenesis, contributing to assembly of a functional 3D tissue. Given that primary VF mucosal cells are not currently available for large-scale clinical therapeutics, utilization of alternative cell populations, such as embryonic stem cell derivatives¹⁵, bone marrow-^44-46 or adipose-derived stem cells^16,17,47, or non-VF somatic cells⁴⁸, is an attractive future direction. Such cells might be differentiated towards a VF mucosal cell phenotype, and primed for successful VF organotypic culture and transplantation, by exposure to defined tensile and vibratory forces in a laryngeal bioreactor^49-51.

VF vibratory function and mucosal wave travel are highly dependent on tissue viscoelasticity²⁰, which in turn is a direct function of VFF-secreted ECM composition^29,30. We seeded cells in a relatively simple collagen, type I-based scaffold material, selected for its cytocompatibility⁴³, high abundance in native human VF mucosa²⁶, and utility in previous organotypic culture systems^22,23 and tissue engineering applications⁴³. This initial collagen matrix was augmented by endogenous ECM production by the seeded cells, and the engineered VF mucosa was further strengthened by VFF-driven contraction to the point that it was able to withstand physiologically relevant aerodynamic driving pressures (˜1-3 kPa) and tissue vibration rates (˜100-300 Hz) for accumulated time doses of 10-15 min during our ex vivo experiments.

Still, while physiologic function was comparable, the engineered VF mucosa did not show lamina propria fiber complexity equivalent to mature human VF mucosa. This observation is not surprising considering that, during human development, differentiation of the VF lamina propria ECM into a complex structure with depth-dependent fibrous protein distribution begins at postnatal age 2 months and is not complete until at least 13 years (y)⁵². Extended culture time, phonation-relevant dosing with mechanical forces (as noted above)^49-51, and/or the use of more architecturally relevant scaffold materials such as decellularized VF mucosa⁵³, may provide the additional microenvironmental cues needed to yield even greater maturation in vitro.

The engineered VF mucosa exhibited low immunogenicity when presented to the human adaptive immune system in vivo, suggesting that this primary cell-based approach itself has clinical potential in an allotransplantation scenario. Full development of this approach may require additional orthotopic transplantation experiments, with evaluation of long-term tolerance and physiologic outcomes. These experiments will allow the skilled artisan to determine the optimal nature and length of in vitro organotypic culture prior to transplantation, and the extent to which subsequent in vivo incorporation of host cells and their participation in ongoing ECM remodeling completes the regeneration process.

Free mucosal graft is a technically straightforward procedure in laryngeal reconstruction and, given its limited vascular demand, has high viability³⁹. Key considerations in determining in vivo outcomes, therefore, may be the relative long-term immunoprivilege of the (primary or non-primary) cells used for organotypic culture, as well as the cumulative impact of the host response and local biomechanical environment on long-term graft remodeling.

In summary, this Example provides a framework for the efficient generation of physiologically relevant and clinically useful bioengineered VF mucosae. Beyond its direct potential for therapeutic use, this approach has application to the development of advanced in vitro model systems for VF mucosal disease modeling and preclinical testing of therapeutic agents.

Materials and Methods

Cell Isolation and Culture.

Human VF mucosae (n=10) were obtained from cadavers at autopsy (<6 h post-mortem) and patients undergoing total laryngectomy with no evidence of VF mucosal disease on routine clinical, endoscopic and radiographic work-up. Procurement was performed with approval of the University of Wisconsin-Madison Health Sciences Institutional Review Board (IRB). Each mucosa was microdissected from its underlying thyroaytenoid muscle, minced with scalpels, and incubated in PBS containing 7.5 mg·mL⁻¹ collagenase, type I (Wako) and 0.5 mg·mL⁻¹ DNase I (Sigma-Aldrich) at 37° C. using 50 Hz agitation for 1 h. Cells released from the ECM were strained through a 40 μm filter (BD Biosciences), rinsed and resuspended in fibroblast-oriented medium (DMEM containing 10% FBS and 100 U·mL⁻¹ antibiotic-antimycotic solution; Sigma-Aldrich), seeded on culture plates pre-coated with Earle's balanced salt solution (EBSS) containing 30 μg·mL⁻¹ collagen, type I, 10 μg·mL⁻¹ fibronectin and 10 μg·mL⁻¹ BSA⁵⁴, and incubated at 37° C. in 5% CO₂. After 30 min, free-floating non-adherent cells were collected, rinsed in epithelial cell-oriented medium (DMEM/Ham's F-12 supplemented with 15 μg·mL⁻¹ bovine pituitary extract, 10 ng·mL⁻¹ epidermal growth factor, 0.5 μg·mL⁻¹ epinephrine, 5 μg·mL⁻¹ insulin, 10 μg·mL⁻¹ transferrin, 10 ng·mL⁻¹ triiodo-L-thyronine, 0.5 μg·mL⁻¹ hydrocortisone, 0.1 ng·mL⁻¹ retinoic acid, 1.5 μg·mL⁻¹ albumin, 100 U·mL⁻¹ antibiotic-antimycotic solution, and 1% FBS; Sigma-Aldrich)⁵⁴, reseeded on pre-coated culture plates, and maintained in epithelial cell-oriented culture conditions. Adherent cells were maintained in fibroblast-oriented culture conditions.

Both cell populations were cultured at 37° C. in 5% CO₂ and passaged when 80% confluent. Given that epithelial cells, once attached, are more adherent than fibroblasts in 2D culture, we performed additional adherence-based VFE purification at passages 1 and 2, as follows. Cells were incubated with 0.05% trypsin-EDTA (Sigma-Aldrich) at 37° C. for 1 minute to detach contaminating VFF, rinsed with PBS, and then incubated with 0.25% trypsin-EDTA at 37° C. for 2 min to detach remaining VFE for subsequent passage. Cell passage was performed using 5×10³ cells·cm⁻² seeding density. Cells intended for fixation and staining were seeded at 1-3×10³ cells·cm⁻² density on slide chambers.

Cell Growth Kinetics.

Passage 1-6 VFF and VFE (n=4 biological replicates per condition) were plated at 3×10⁵ cells·cm⁻² seeding density in 6-well plates and cultured at 37° C. in 5% CO₂. Cells were trypsinized and harvested after 96 hours, stained with trypan blue, and counted. All counts (at the time of cell seeding and harvest) were performed using a hematocytometer in technical quadruplicate. Cell plates were imaged to confirm cell trypsinization and complete detachment. Population doubling time was calculated as 2^N=C_f/C_i, where N denotes doubling time, C_f denotes the final cell count at time of harvest, and C, denotes the initial cell count at time of seeding⁵⁵.

3D Organotypic Culture.

We engineered 167 VF mucosae using 3D organotypic culture. Purified rat tail collagen, type I (BD Biosciences) was prepared to a final concentration of 2.4 mg·mL⁻¹ according to the manufacturer's instructions and seeded with 2×10⁵ VFF·mL⁻¹. Mucosae used for transmucosal resistance experiments were prepared with a reduced fibroblast seeding density of 5.6×10⁴ VFF·mL⁻¹ to reduce scaffold contraction and eliminate detachment from the insert wall. The cell-scaffold mixture was added to the apical chamber of a culture insert (0.14 mL per well of a 24-well insert with 0.4 μm pore size, 0.64 cm diameter, 0.3 cm² surface area; or 2.0 mL per well of a 6-well insert with 0.4 μm pore size, 2.31 cm diameter, 4.2 cm² surface area; BD Biosciences). The collagen-based scaffold was then polymerized at 37° C. for 40 min. Fibroblast-oriented medium was added to both apical and basolateral chambers and the cells were cultured for 24 hours. Next, VFE were seeded on the polymerized scaffold surface within the apical chamber (2×10⁵ VFE per well of a 24-well insert; 2×10⁶ VFE per well of a 6-well insert) and cultured in epithelial cell-oriented medium; a 1:1 ratio of fibroblast- and epithelial cell-oriented media was added to the basolateral chamber. After an additional 48 h, the epithelial cell-oriented medium was aspirated from the apical chamber, leaving VFE at the air-liquid interface. We continued organotypic culture for a total of 8-28 d with basolateral chamber media change every 48 h.

As initial histological assessment showed no difference in engineered VF mucosa morphology at 14 and 28 d, we performed all subsequent histological, immunohistochemical, proteomic, physiologic and immunologic assays on samples harvested at 14 d. Experimental comparisons involving the scaffold only, VFF in scaffold and VFE on scaffold involved identical culture conditions for the entire 14 d period.

Ex Vivo Physiologic Experiments.

Ex vivo physiologic data were collected using a previously reported experimental setup⁵⁶. HSDI data were captured at 13,500 frames·s⁻¹ and calibrated for distance measurement in mm. Acoustic data were digitized at 48 kHz with 16-bit quantization. The microphone and sound level meter (dB A-weighted) were positioned 3 cm from the glottal midpoint.

Previously harvested and cryopreserved canine larynges (n=10) were thawed at 4° C. overnight and the epiglottis, aryepiglottic folds and false VFs were removed to maximize visual exposure of the true VFs. Arytenoid adduction procedures were performed using 5-0 sutures. Following instrument calibration, each larynx was mounted and then subjected to gradual increases in P_s to obtain P_th. Aerodynamic, acoustic and HSDI data were then collected at P_th, 1.5P_th and 2P_th. For 5 larynges, data were collected under native conditions, following unilateral VF mucosa resection, and following engineered VF mucosa placement. Additional data were collected in a parallel experiment using the remaining 5 larynges and previously cryopreserved human oral mucosa (n=5; harvested from cadavers at autopsy [<6 h post-mortem] under IRB exemption) in place of the engineered VF mucosa. The engineered VF or oral mucosa was attached to the underlying thyroarytenoid muscle using fibrin sealant (Tisseel; Baxter). All ex vivo tissues were draped with saline-soaked gauze between experimental runs to prevent dehydration.

_ac was derived from acoustic intensity in dB SPL, based on an assumption of uniform acoustic radiation from the glottis in all directions. _aero was calculated by multiplying P_s in kPa by U in L·s¹. E_g was calculated by dividing _ac by _aero (both in W) and converting to a percentage. HSDI-based analyses of glottal area and vibratory phase differences were performed in Matlab (Mathworks) with implementation of previously described algorithms^57-60. Acoustic time-domain plots and narrowband spectrograms (150 ms analysis window) were generated using Praat 5.3.41 (Paul Boersma and David Weenink, University of Amsterdam). Phase plots were generated as previously described⁶¹. Qualitative signal typing was performed using previously reported criteria^36,37.

Humanized Mouse Experiments.

hPBL were obtained with IRB approval and isolated from individuals with no known hematopoietic disorders via either leukapheresis using lymphocyte separation medium (Cellgro) or routine venipuncture with collection in a lavender-top Vacutainer® tube (BD Biosciences) followed by centrifugation and separation. All samples were subject to ACK lysis of red blood cells and frozen prior to experimental use.

NOD-scid IL2rγ^null (NSG) mice aged 7-8 weeks (wk) were used for all in vivo experiments (n=23; Jackson Laboratory); protocols were approved by the University of Wisconsin School of Medicine and Public Health Animal Care and Use Committee. Mice were conditioned with sublethal (2.5 Gy) total body irradiation; 4 h following irradiation, engineered VF mucosae were implanted under the bilateral renal capsules. The following day, mice received an intravenous injection of 13×10⁶ hPBL that were isolated from an original laryngeal tissue donor and autologous to one of the two engineered VF mucosal grafts. Additional mice were subject to the following conditions: engineered VF mucosal graft implantation followed by delivery of hPBL that were allogeneic to both grafts; engineered VF mucosal graft implantation followed by no hPBL delivery; acellular scaffold implantation followed by hPBL delivery; acellular scaffold implantation followed by no hPBL delivery (n=3-6 per condition).

Mice were monitored every 1-2 d for decrease in body mass and clinical signs consistent with GVHD, such as a hunched posture, ruffled fur, and mobility difficulty: mice who received hPBL were euthanized in response to a >15% decrease in body mass compared to baseline. Other mice were monitored for 70-98 d. Peripheral blood was collected at 7 d post-engraftment and at the experimental endpoint and processed for flow cytometry. Auto- and allografts, acellular scaffolds and eyelids (used as a positive control for xenogeneic GVHD) were harvested at the experimental endpoint and processed for histology and immunohistochemisty.

Flow Cytometry.

Cells were washed and suspended in staining buffer (Hanks' balanced salt solution (HBSS) containing 2% FBS and 10 mM HEPES). For cell surface staining, single cell suspensions were incubated with fluorochrome-conjugated antibodies. For intracellular staining, unstained or surface-stained cells were washed, then fixed and permeabilized using Cytofix/Cytoperm™ (BD Biosciences) according to the manufacturer's instructions. The permeabilized cells were then incubated with fluorochrome-conjugated antibodies against the intracellular targets, washed, and resuspended in staining buffer. All antibodies are listed in Table 6. Cells were also incubated with DAPI: a change in DAPI fluorescent intensity was used to gate live versus dead cells. Samples were run on a 4-laser, 14-color LSR II instrument (BD Biosciences) and data were analyzed using FlowJo 8.7.1 (Tree Star). We employed both isotype (Table 6) and fluorescence-minus-one (FMO) controls.

Histology, Immunocytochemistry and Immunohistochemistry.

Cells seeded on slide chambers were harvested after 2-3 days in culture and fixed using 2-4% paraformaldehyde. Native and engineered tissues were processed for paraffin (5 μm-thick) and frozen (8 μm-thick) sections. Routine H&E, Alcian blue (pH 2.5, with and without hyaluronidase digestion), Elastic van Gieson, and Movat's pentachrome histological staining was performed on both paraffin and frozen sections to evaluate morphology.

Frozen sections used for immunostaining were incubated with 0.5% Triton X-100 for 15 min, Image iT-FX (Life Technologies) for 30 min, and Block-Ace (AbD Serotech) and 5% donkey serum (Sigma-Aldrich) for 30 min. Fixed cells and sections were incubated with primary antibodies for 90 min followed by appropriate secondary antibodies for 90 min, with thorough wash steps between each incubation step. A complete list of antibodies is provided in Table 7. Following primary and secondary antibody incubations, cells and sections were counterstained with DAPI, covered with Vectashield antifade mounting medium (Vector Labs), and coverslipped.

Paraffin sections used for immunostaining were first processed for antigen retrieval in a decloaking chamber (Biocare Medical) using 10 mM citrate buffer (pH 6.0). Sections were permeabilized using 0.2% Triton X-100 for 10 minutes and incubated with 10% BSA in PBS for 60 minutes to block non-specific binding, prior to incubation with primary antibodies for 60 minutes (Table 7). Thorough washing was performed between each incubation step. For HRP-based detection, endogenous peroxidase was quenched using 3% hydrogen peroxide in PBS. ImmPRESS anti-mouse and anti-rabbit Ig HRP polymers were used for secondary detection (30 minutes incubation) and the ImmPACT DAB kit was used to develop the signal (all reagents from Vector Labs), according to the manufacturer's instructions. Sections were counterstained with hematoxylin, dehydrated, cleared and coverslipped.

All staining protocols were performed using 5-10 biological replicates, each with two technical replicates. Histological, immunocytochemical and immunohistochemical images were captured using a microscope (E-600; Nikon) equipped with a digital microscopy camera (DP-71; Olympus). Consistent exposure parameters were used for each immunostained protein of interest. Positive control tissues (Table 7) showed expected immunostaining patterns. Negative control sections, stained with an IgG isotype control or without the primary or secondary antibody, showed no immunoreactivity.

Histology- and immunohistochemistry-based cell counts were performed using 10 non-overlapping, high-magnification fields per biological replicate. For measurement of overall cell density in the engineered and native VF mucosae, 0.25 mm²-sized fields were randomly sampled from the central lamina propria region. For measurement of hCD4⁺hFOXP3⁺ cell density in the VF auto- and allografts, 0.1 mm²-sized fields were randomly sampled from the deep lamina propria region of each graft (nearest the mouse kidney) as this region contained the greatest number of infiltrating cells. For measurement of hCD4⁺hFOXP3⁺ cell density in the mouse eyelid, 0.1 mm²-sized fields were randomly sampled from the meibomian gland-containing region.

Proteomic Analyses.

Proteins were extracted from each sample (n=3 biological replicates per condition) by first adding 150 μL of 4% SDS, 0.1 M Tris-HCl (pH 7.6) and 0.1 M dithiothreitol. Next, samples were sonicated (alternating 20 s on/off cycles for 6 min) using a probe sonicator (XL2015 with PN/418 microtip; Misonix), heated to 95° C. for 7 min, and then centrifuged at 16,100 g at 20° C. for 5 min. A 30 μL aliquot of the supernatant was processed according to the filter-aided sample preparation (FASP) protocol for SDS removal and on-filter digestion^62,63. Briefly, the supernatant was added to a 30,000 MWCO Vivacon 500 filter (Sartorius), washed, alkylated, and digested with trypsin (50:1 w/w protein-to-trypsin ratio) at 37° C. overnight. The digested sample was desalted using a Sep-Pak C18 1 cc Vac cartridge (Waters), evaporated to dryness in a vacuum centrifuge, and reconstituted in 5% acetonitrile and 2% formic acid in water.

The following mass spectrometry (MS) experiment was performed using two technical replicates per biological replicate. Approximately 1.2 μg of the protein digest, as estimated by a BCA assay (Pierce), was injected into a Waters nanoAcquity HPLC coupled to an ESI ion-trap/orbitrap mass spectrometer (LTQ Orbitrap Velos; Thermo Scientific). Peptides were separated on a 100 μm-inner-diameter column packed with 20 cm of 3 μm MAGIC aqC18 beads (Bruker-Michrom), which were packed against an in-house laser-pulled tip, and eluted at 0.3 μL·min⁻¹ in 0.1% formic acid with a gradient of increasing acetonitrile, over 2.5 h. A full-mass scan (300-1500 m/z) was performed in the orbitrap at a resolution of 60,000. The ten most intense peaks were then selected for fragmentation by high-energy collisional dissociation (HCD) at 42% collision energy, with a resolution of 7500 and isolation width of 2.5 m/z. Dynamic exclusion was enabled with a repeat count of 2 over 30 seconds (s) and an exclusion duration of 120 s.

We searched the mass spectra against appropriate organism protein databases (Homo sapiens and Rattus norvegicus; UniProt) using the SEQUEST algorithm within Proteome Discoverer (Thermo Scientific). We allowed two missed cleavages, required at least two unique peptides per protein identification, and filtered the results using a 1% peptide false discovery rate. Precursor mass tolerance was set to 25 ppm and 0.05 Da for fragment ion tolerance. Variable methionine and proline oxidation (+15.995 Da) and static carbamidomethylation of cysteines (+57.021 Da) were also used. Spectral counting-based protein quantification was performed using the normalized spectral abundance factor (NSAF) approach²⁸. Further normalization, based on an assumption of comparable degradation of rat collagen across experimental conditions, was performed using a correction factor calculated from the NSAF values of the 10 most abundant proteins in the collagen, type I-based scaffold. The raw MS data files may be downloaded from the PeptideAtlas peptide data repository⁶⁴ using the dataset identifier PASS00271.

Protein Array.

Passage 3 VFF were seeded at a density of 2×10⁵ cells·mL⁻¹ collagen, type I and cultured at 37° C. in 5% CO₂ for 96 h. Fibroblast-oriented medium was changed every 24 h. Culture supernatants were harvested 48 and 96 h post-seeding, pooled across 6 biological replicates per time point, and processed for measurement of MMP/TIMP concentrations using the Quantibody® Human MMP Array 1 platform (RayBiotech), according to the manufacturer's instructions. Medium only was used as a negative control. Each MMP/TIMP of interest was assayed in technical quadruplicate. The array was scanned and data processed using Q-Analyzer software (RayBiotech).

Rheologic Analyses.

Small-amplitude oscillatory shear measurements (n=4-12 biological replicates per condition) were performed in a Bohlin C-VOR rheometer (Malvern) using 15-mm parallel-plate geometry with a 0.3-0.6 mm gap size (adjusted according to the sample volume) at 37° C. Serrated plates were used to avoid sample slippage during testing. Frequency sweep tests were performed from 0.01-10 Hz under a constant applied stress of 3 Pa, which was determined as the linear viscoelastic limit via stress sweep tests.

Transmucosal Electrical Resistance Analyses.

Electrical resistance measurements (n=4 biological replicates per condition, each with two technical replicates) were performed in 24-well inserts using a Millicell-ERS volt-ohm meter (Millipore), according to the manufacturer's instructions. Samples were rinsed and fresh media were added to the basolateral and apical insert chambers 15-30 min prior to data collection. The resistance value of an insert containing fresh media but no scaffold or cells was subtracted from each sample measurement. Resistance values were then further normalized to the scaffold only condition.

Statistical Analyses.

Technical replicates were averaged and all statistical comparisons were performed using independent biological replicates. NSAF-based quantitative proteomic data were analyzed using a two-tailed Student's t test with implementation of Benjamin-Hochberg correction⁶⁵ to account for multiple testing. Gene ontology term enrichment analysis was performed using the BiNGO⁶⁶ (hypergeometric model with Benjamini-Hochberg correction) and REViGO⁶⁷ (SimRel cutoff=0.4) algorithms. Ontology term enrichment schematics were generated using Cytoscape 2.8.2⁶⁸. Other statistical testing was performed using SAS 9.2 (SAS Institute). Flow cytometry, rheology (comparison of slopes of each fitted curve), cell density, transmucosal resistance, P_th, and body mass data were analyzed using one-way ANOVA. Glottal area and vibratory phase data were analyzed using two-way ANOVA, with VF mucosa condition and P_th as fixed effects, and their interaction term included. Cell proliferation data were also analyzed using two-way ANOVA, with cell type and culture passage as fixed effects, and their interaction term included. Data were first evaluated for normality and equality of variance using visual inspection of raw data plots and Levene's test; data were rank-transformed where needed to meet the equal variance assumptions of the Student's t test and ANOVA. In all ANOVA models, if the omnibus F test revealed a significant difference, pairwise comparisons were performed using Fisher's protected least significant difference method. Categorical acoustic signal typing data were analyzed using a chi-squared test. An initial (pre-correction) type I error rate of 0.01 was used for all statistical testing; quantitative proteomic data were subject to an additional fold change cutoff of 4. All P-values were two-sided.

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TABLE 1
Lists of proteins identified in LC-MS/MS analysis of scaffold only, VFF in scaffold, VFE on scaffold, engineered VF mucosa and native
VF mucosa. Identifications were based on a 1% false discovery rate. The scaffold was sourced from Rattus norvegicus; VFF, VFE and
native VF mucosa were sourced from Homo sapiens. Proteins are listed in order of descending peptide spectra matches.
UniProt					Sequence		Peptide
accession					coverage	Unique	spectra
number	Protein name	Gene symbol	Organism	Score	(%)	peptides	matches
Scaffold only
P02454	Collagen alpha-1(I) chain	COL1A1	Rattus norvegicus	14562.52	74.88	79	16858
P02466	Collagen alpha-2(I) chain	COL1A2	Rattus norvegicus	10402.75	69.39	68	8327
P13941	Collagen alpha-1(III) chain	COL3A1	Rattus norvegicus	475.78	11.41	8	456
Q29RW1	Myosin-4	MYH4	Rattus norvegicus	414.90	15.58	15	101
P02770	Serum albumin	ALB	Rattus norvegicus	285.30	25.99	14	74
P50609	Fibromodulin	FMOD	Rattus norvegicus	255.20	19.68	5	58
P04937	Fibronectin	FN1	Rattus norvegicus	167.39	5.41	9	45
P68035	Actin, alpha cardiac muscle 1	ACTC1	Rattus norvegicus	158.07	19.63	3	39
P60711	Actin, cytoplasmic 1	ACTB	Rattus norvegicus	113.85	20.53	3	28
P01026	Complement C3	C3	Rattus norvegicus	89.00	2.53	3	25
Q6IFW6	Keratin, type I cytoskeletal 10	KRT10	Rattus norvegicus	81.38	9.89	5	24
P11517	Hemoglobin subunit beta-2	HBB2	Rattus norvegicus	66.34	40.14	5	23
P00564	Creatine kinase M-type	CKM	Rattus norvegicus	92.71	20.21	6	22
Q01129	Decorin	DCN	Rattus norvegicus	78.80	15.25	4	21
P04797	Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	Rattus norvegicus	42.24	14.71	3	19
P63018	Heat shock cognate 71 kDa protein	HSPA8	Rattus norvegicus	70.47	7.59	5	19
Q6IMF3	Keratin, type II cytoskeletal 1	KRT1	Rattus norvegicus	64.27	5.60	3	19
P01946	Hemoglobin subunit alpha-1/2	HBA1	Rattus norvegicus	74.27	35.21	3	17
P47853	Biglycan	BGN	Rattus norvegicus	50.62	8.94	2	16
P62260	14-3-3 protein epsilon	YWHAE	Rattus norvegicus	44.11	8.63	2	15
Q68FP1	Gelsolin	GSN	Rattus norvegicus	49.45	3.85	2	13
Q6P6Q2	Keratin, type II cytoskeletal 5	KRT5	Rattus norvegicus	42.90	4.17	2	13
P04462	Myosin-8 (Fragment)	MYH8	Rattus norvegicus	32.58	19.07	2	11
Q9Z1P2	Alpha-actinin-1	ACTN1	Rattus norvegicus	33.89	5.16	4	11
Q62812	Myosin-9	MYH9	Rattus norvegicus	33.15	3.21	5	10
P07335	Creatine kinase B-type	CKB	Rattus norvegicus	24.32	5.51	2	9
P34058	Heat shock protein HSP 90-beta	HSP90AB1	Rattus norvegicus	21.83	5.39	3	7
P48037	Annexin A6	ANXA6	Rattus norvegicus	13.18	4.31	3	5
Q63041	Alpha-1-macroglobulin	A1M	Rattus norvegicus	20.75	2.27	2	5
P11980	Pyruvate kinase isozymes M1/M2	PKM	Rattus norvegicus	17.00	7.53	2	4
B0BNI5	Olfactomedin-like protein 3	OLFML3	Rattus norvegicus	15.93	9.85	2	3
P04642	L-lactate dehydrogenase A chain	LDHA	Rattus norvegicus	11.00	7.53	2	3
VFF in scaffold
P02454	Collagen alpha-1(I) chain	COL1A1	Rattus norvegicus	19037.82	70.06	82	23558
P02452	Collagen alpha-1(I) chain	COL1A1	Homo sapiens	9435.37	51.91	54	12141
P02466	Collagen alpha-2(I) chain	COL1A2	Rattus norvegicus	13087.50	67.49	67	11240
P08123	Collagen alpha-2(I) chain	COL1A2	Homo sapiens	3527.28	43.70	37	3038
Q09666	Neuroblast differentiation-associated protein	AHNAK	Homo sapiens	3901.70	45.53	121	1159
	AHNAK
P02751	Fibronectin	FN1	Homo sapiens	2589.18	36.92	59	665
P12111	Collagen alpha-3(VI) chain	COL6A3	Homo sapiens	1935.31	24.99	59	587
P21333	Filamin-A	FLNA	Homo sapiens	2125.93	37.97	65	524
P08670	Vimentin	VIM	Homo sapiens	1767.03	59.23	34	518
P60711	Actin, cytoplasmic 1	ACTB	Rattus norvegicus	1522.82	63.20	16	411
P35579	Myosin-9	MYH9	Homo sapiens	1580.80	37.96	56	396
P12109	Collagen alpha-1(VI) chain	COL6A1	Homo sapiens	901.30	23.35	15	378
P14618	Pyruvate kinase PKM	PKM	Homo sapiens	1333.05	59.70	26	356
P07355	Annexin A2	ANXA2	Homo sapiens	1335.76	58.11	20	350
Q15149	Plectin	PLEC	Homo sapiens	1144.56	19.75	69	329
P02545	Prelamin-A/C [Cleaved into: Lamin-A/C]	LMNA	Homo sapiens	1094.53	54.67	35	326
P02458	Collagen alpha-1(II) chain	COL2A1	Homo sapiens	430.06	7.37	5	305
P07437	Tubulin beta chain	TUBB	Homo sapiens	1015.36	66.89	21	281
Q00610	Clathrin heavy chain 1	CLTC	Homo sapiens	1081.13	27.22	36	268
O43707	Alpha-actinin-4	ACTN4	Homo sapiens	1000.62	51.37	36	265
P68371	Tubulin beta-4B chain	TUBB4B	Homo sapiens	967.12	66.74	21	262
P11021	78 kDa glucose-regulated protein	HSPA5	Homo sapiens	866.31	33.33	18	250
P08238	Heat shock protein HSP 90-beta	HSP90AB1	Homo sapiens	880.50	33.01	18	238
P11142	Heat shock cognate 71 kDa protein	HSPA8	Homo sapiens	853.97	39.01	22	236
P04406	Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	Homo sapiens	790.07	46.87	12	235
Q13885	Tubulin beta-2A chain	TUBB2A	Homo sapiens	780.66	56.63	18	221
P15144	Aminopeptidase N	ANPEP	Homo sapiens	776.75	25.54	23	217
P13941	Collagen alpha-1(III) chain	COL3A1	Rattus norvegicus	235.58	12.03	11	217
P12814	Alpha-actinin-1	ACTN1	Homo sapiens	771.27	36.77	27	215
P68363	Tubulin alpha-1B chain	TUBA1B	Homo sapiens	753.76	50.55	18	215
Q13509	Tubulin beta-3 chain	TUBB3	Homo sapiens	668.44	36.89	14	201
P00558	Phosphoglycerate kinase 1	PGK1	Homo sapiens	755.19	52.04	17	199
P04083	Annexin A1	ANXA1	Homo sapiens	758.14	52.60	16	192
P68104	Elongation factor 1-alpha 1	EEF1A1	Homo sapiens	761.73	33.98	11	188
P68035	Actin, alpha cardiac muscle 1	ACTC1	Rattus norvegicus	611.13	32.89	10	188
P07900	Heat shock protein HSP 90-alpha	HSP90AA1	Homo sapiens	649.34	21.31	12	183
P05539	Collagen alpha-1(II) chain	COL2A1	Rattus norvegicus	293.86	5.50	4	177
Q9Y490	Talin-1	TLN1	Homo sapiens	638.77	19.91	31	168
P06576	ATP synthase subunit beta, mitochondrial	ATP5B	Homo sapiens	584.55	39.89	14	161
P06733	Alpha-enolase	ENO1	Homo sapiens	521.99	39.86	13	147
P14625	Endoplasmin	HSP90B1	Homo sapiens	515.39	25.28	15	144
P08758	Annexin A5	ANXA5	Homo sapiens	478.42	55.31	15	141
Q14764	Major vault protein	MVP	Homo sapiens	443.98	31.35	19	132
P50454	Serpin H1	SERPINH1	Homo sapiens	533.29	41.15	13	131
P04264	Keratin, type II cytoskeletal 1	KRT1	Homo sapiens	429.54	28.88	18	125
P07585	Decorin	DCN	Homo sapiens	397.67	43.18	12	123
Q9BUF5	Tubulin beta-6 chain	TUBB6	Homo sapiens	397.03	33.86	13	122
P46940	Ras GTPase-activating-like protein IQGAP1	IQGAP1	Homo sapiens	441.43	19.01	21	121
P62805	Histone H4	HIST1H4A	Homo sapiens	385.21	51.46	6	116
P04075	Fructose-bisphosphate aldolase A	ALDOA	Homo sapiens	494.95	57.69	14	114
O43852	Calumenin	CALU	Homo sapiens	447.18	44.76	14	114
O60814	Histone H2B type 1-K	HIST1H2BK	Homo sapiens	314.19	34.92	4	112
P08133	Annexin A6	ANXA6	Homo sapiens	404.62	33.73	18	111
P11216	Glycogen phosphorylase, brain form	PYGB	Homo sapiens	415.67	28.23	19	110
P62937	Peptidyl-prolyl cis-trans isomerase A	PPIA	Homo sapiens	393.57	55.76	9	110
Q07065	Cytoskeleton-associated protein 4	CKAP4	Homo sapiens	378.99	38.70	18	105
P63104	14-3-3 protein zeta/delta	YWHAZ	Homo sapiens	352.91	37.14	8	103
Q14315	Filamin-C	FLNC	Homo sapiens	456.37	11.12	18	101
P07237	Protein disulfide-isomerase	P4HB	Homo sapiens	383.52	35.63	15	101
P00338	L-lactate dehydrogenase A chain	LDHA	Homo sapiens	340.74	37.95	11	101
P13639	Elongation factor 2	EEF2	Homo sapiens	332.84	28.90	21	101
P18669	Phosphoglycerate mutase 1	PGAM1	Homo sapiens	324.02	44.88	9	99
P11413	Glucose-6-phosphate 1-dehydrogenase	G6PD	Homo sapiens	375.31	27.57	10	98
P67936	Tropomyosin alpha-4 chain	TPM4	Homo sapiens	340.33	47.58	13	98
P60174	Triosephosphate isomerase	TPI1	Homo sapiens	367.86	61.19	12	97
P12110	Collagen alpha-2(VI) chain	COL6A2	Homo sapiens	329.15	22.37	17	96
P18206	Vinculin	VCL	Homo sapiens	294.56	26.81	21	94
Q06830	Peroxiredoxin-1	PRDX1	Homo sapiens	397.52	52.26	9	93
Q6NZI2	Polymerase I and transcript release factor	PTRF	Homo sapiens	339.44	28.21	9	92
P10809	60 kDa heat shock protein, mitochondrial	HSPD1	Homo sapiens	336.47	28.27	13	91
P25705	ATP synthase subunit alpha, mitochondrial	ATP5A1	Homo sapiens	321.89	20.43	8	91
P08107	Heat shock 70 kDa protein 1A/1B	HSPA1A	Homo sapiens	300.85	25.12	11	87
P62260	14-3-3 protein epsilon	YWHAE	Rattus norvegicus	264.94	40.00	8	87
P06396	Gelsolin	GSN	Homo sapiens	359.55	21.10	12	86
P27348	14-3-3 protein theta	YWHAQ	Homo sapiens	294.97	38.37	7	86
P30101	Protein disulfide-isomerase A3	PDIA3	Homo sapiens	293.81	28.12	11	86
Q99536	Synaptic vesicle membrane protein VAT-1	VAT1	Homo sapiens	294.87	29.77	7	85
	homolog
P23284	Peptidyl-prolyl cis-trans isomerase B	PPIB	Homo sapiens	268.90	40.28	9	85
O00299	Chloride intracellular channel protein 1	CLIC1	Homo sapiens	342.42	63.90	11	84
P09382	Galectin-1	LGALS1	Homo sapiens	300.65	46.67	5	84
P02768	Serum albumin	ALB	Homo sapiens	319.64	6.73	4	83
P55072	Transitional endoplasmic reticulum ATPase	VCP	Homo sapiens	344.40	26.30	14	82
P29401	Transketolase	TKT	Homo sapiens	331.79	28.09	12	81
P13489	Ribonuclease inhibitor	RNH1	Homo sapiens	320.57	33.84	10	80
P02461	Collagen alpha-1(III) chain	COL3A1	Homo sapiens	119.06	9.75	9	77
Q13813	Spectrin alpha chain, non-erythrocytic 1	SPTAN1	Homo sapiens	287.37	12.46	21	76
Q16555	Dihydropyrimidinase-related protein 2	DPYSL2	Homo sapiens	285.66	35.14	12	74
P38646	Stress-70 protein, mitochondrial	HSPA9	Homo sapiens	275.02	17.23	9	74
Q04828	Aldo-keto reductase family 1 member C1	AKR1C1	Homo sapiens	282.79	35.91	8	73
Q9NZN4	EH domain-containing protein 2	EHD2	Homo sapiens	275.71	38.31	15	73
P27797	Calreticulin	CALR	Homo sapiens	295.72	37.41	9	72
P63244	Guanine nucleotide-binding protein subunit	GNB2L1	Homo sapiens	254.12	55.84	11	72
	beta-2-like 1
Q99715	Collagen alpha-1(XII) chain	COL12A1	Homo sapiens	251.62	8.23	18	72
P49368	T-complex protein 1 subunit gamma	CCT3	Homo sapiens	251.20	29.17	11	72
P53396	ATP-citrate synthase	ACLY	Homo sapiens	254.08	16.71	12	71
P07195	L-lactate dehydrogenase B chain	LDHB	Homo sapiens	228.06	37.43	10	70
P49327	Fatty acid synthase	FASN	Homo sapiens	276.72	11.87	18	69
P23528	Cofilin-1	CFL1	Homo sapiens	283.28	56.02	8	68
Q14204	Cytoplasmic dynein 1 heavy chain 1	DYNC1H1	Homo sapiens	240.66	6.03	21	67
P51149	Ras-related protein Rab-7a	RAB7A	Homo sapiens	226.35	47.83	9	66
P09211	Glutathione S-transferase P	GSTP1	Homo sapiens	284.60	48.57	6	65
P02765	Alpha-2-HS-glycoprotein	AHSG	Homo sapiens	248.58	5.45	3	64
O60664	Perilipin-3	PLIN3	Homo sapiens	227.81	30.88	8	62
P37802	Transgelin-2	TAGLN2	Homo sapiens	217.26	41.21	7	59
P21980	Protein-glutamine gamma-glutamyltransferase	TGM2	Homo sapiens	200.88	13.54	6	58
	2
P08865	40S ribosomal protein SA	RPSA	Homo sapiens	228.76	31.86	7	57
Q03135	Caveolin-1	CAV1	Homo sapiens	206.84	63.48	9	57
P40926	Malate dehydrogenase, mitochondrial	MDH2	Homo sapiens	223.19	36.69	9	56
P27824	Calnexin	CANX	Homo sapiens	209.31	15.37	6	56
P60660	Myosin light polypeptide 6	MYL6	Homo sapiens	179.26	39.07	5	56
P11766	Alcohol dehydrogenase class-3	ADH5	Homo sapiens	256.32	22.99	6	55
P31946	14-3-3 protein beta/alpha	YWHAB	Homo sapiens	197.15	28.46	5	55
P04792	Heat shock protein beta-1	HSPB1	Homo sapiens	193.74	49.76	7	55
P07737	Profilin-1	PFN1	Homo sapiens	166.70	63.57	7	55
P21796	Voltage-dependent anion-selective channel	VDAC1	Homo sapiens	235.02	27.21	6	54
	protein 1
P05387	60S acidic ribosomal protein P2	RPLP2	Homo sapiens	228.69	69.57	4	54
P16152	Carbonyl reductase [NADPH] 1	CBR1	Homo sapiens	227.14	41.16	7	54
P05388	60S acidic ribosomal protein P0	RPLP0	Homo sapiens	222.31	35.02	7	54
Q9H299	SH3 domain-binding glutamic acid-rich-like	SH3BGRL3	Homo sapiens	199.84	31.18	3	54
	protein 3
Q15084	Protein disulfide-isomerase A6	PDIA6	Homo sapiens	196.61	32.50	9	54
O75083	WD repeat-containing protein 1	WDR1	Homo sapiens	275.17	26.57	8	53
P17301	Integrin alpha-2	ITGA2	Homo sapiens	197.72	11.52	9	53
P46821	Microtubule-associated protein 1B	MAP1B	Homo sapiens	180.13	9.08	14	52
P61158	Actin-related protein 3	ACTR3	Homo sapiens	222.55	37.80	10	51
P26038	Moesin	MSN	Homo sapiens	180.77	19.93	12	51
P35237	Serpin B6	SERPINB6	Homo sapiens	176.70	26.06	8	51
P35908	Keratin, type II cytoskeletal 2 epidermal	KRT2	Homo sapiens	168.33	22.54	11	51
P00387	NADH-cytochrome b5 reductase 3	CYB5R3	Homo sapiens	188.27	31.56	7	50
P61961	14-3-3 protein gamma	YWHAG	Homo sapiens	172.74	24.70	5	50
Q15365	Poly(rC)-binding protein 1	PCBP1	Homo sapiens	172.07	32.02	6	50
P13645	Keratin, type I cytoskeletal 10	KRT10	Homo sapiens	165.38	29.28	12	50
Q14697	Neutral alpha-glucosidase AB	GANAB	Homo sapiens	234.90	10.59	6	49
Q01082	Spectrin beta chain, non-erythrocytic 1	SPTBN1	Homo sapiens	162.63	10.24	16	49
Q9NZM1	Myoferlin	MYOF	Homo sapiens	184.03	9.70	13	48
P22314	Ubiquitin-like modifier-activating enzyme 1	UBA1	Homo sapiens	182.43	15.03	11	48
Q01518	Adenylyl cyclase-associated protein 1	CAP1	Homo sapiens	174.85	17.47	7	48
Q15019	Septin-2	41884	Homo sapiens	171.34	38.23	8	48
P22626	Heterogeneous nuclear ribonucleoproteins A2/B1	HNRNPA2B1	Homo sapiens	156.70	20.11	6	48
P23396	40S ribosomal protein S3	RPS3	Homo sapiens	139.84	38.68	8	48
P04843	Dolichyl-diphosphooligosaccharide--protein	RPN1	Homo sapiens	185.20	23.56	9	47
	glycosyltransferase subunit 1
Q96AG4	Leucine-rich repeat-containing protein 59	LRRC59	Homo sapiens	172.33	23.45	5	47
P21810	Biglycan	BGN	Homo sapiens	183.82	28.80	9	46
P61978	Heterogeneous nuclear ribonucleoprotein K	HNRNPK	Homo sapiens	175.85	28.08	9	46
P61204	ADP-ribosylation factor 3	ARF3	Homo sapiens	150.47	38.67	6	46
P30050	60S ribosomal protein L12	RPL12	Homo sapiens	168.79	54.55	6	45
P30086	Phosphatidylethanolamine-binding protein 1	PEBP1	Homo sapiens	155.97	50.27	5	45
P26641	Elongation factor 1-gamma	EEF1G	Homo sapiens	164.58	21.97	7	44
P12882	Myosin-1	MYH1	Homo sapiens	160.33	8.92	12	44
P62241	40S ribosomal protein S8	RPS8	Homo sapiens	149.78	32.69	5	44
P62424	60S ribosomal protein L7a	RPL7A	Homo sapiens	144.33	18.42	5	44
Q96D15	Reticulocalbin-3	RCN3	Homo sapiens	177.77	21.95	4	43
P36578	60S ribosomal protein L4	RPL4	Homo sapiens	136.85	13.35	5	43
P30041	Peroxiredoxin-6	PRDX6	Homo sapiens	134.23	41.07	8	43
Q92928	Putative Ras-related protein Rab-1C	RAB1C	Homo sapiens	129.95	22.89	4	43
P04844	Dolichyl-diphosphooligosaccharide--protein	RPN2	Homo sapiens	175.85	29.64	10	42
	glycosyltransferase subunit 2
P17655	Calpain-2 catalytic subunit	CAPN2	Homo sapiens	161.98	17.43	8	42
P00367	Glutamate dehydrogenase 1, mitochondrial	GLUD1	Homo sapiens	157.44	25.63	10	42
P49748	Very long-chain specific acyl-CoA	ACADVL	Homo sapiens	156.86	21.07	9	42
	dehydrogenase, mitochondrial
P07339	Cathepsin D	CTSD	Homo sapiens	146.93	20.63	6	42
P09525	Annexin A4	ANXA4	Homo sapiens	135.78	31.03	8	42
Q96AY3	Peptidyl-prolyl cis-trans isomerase FKBP10	FKBP10	Homo sapiens	133.36	11.86	5	42
Q9GZV4	Eukaryotic translation initiation factor 5A-2	EIF5A2	Homo sapiens	164.28	19.61	3	41
P40939	Trifunctional enzyme subunit alpha,	HADHA	Homo sapiens	159.99	17.96	8	41
	mitochondrial
O60701	UDP-glucose 6-dehydrogenase	UGDH	Homo sapiens	145.28	25.71	9	41
P02538	Keratin, type II cytoskeletal 6A	KRT6A	Homo sapiens	139.17	26.77	13	41
P18085	ADP-ribosylation factor 4	ARF4	Homo sapiens	122.95	48.89	8	41
P24534	Elongation factor 1-beta	EEF1B2	Homo sapiens	172.02	20.44	3	40
P68871	Hemoglobin subunit beta	HBB	Homo sapiens	134.50	38.78	5	40
P50990	T-complex protein 1 subunit theta	CCT6	Homo sapiens	134.31	16.97	7	40
P12236	ADP/ATP translocase 3	SLC25A6	Homo sapiens	112.19	27.85	8	40
P09972	Fructose-bisphosphate aldolase C	ALDOC	Homo sapiens	171.14	18.41	4	38
P22392	Nucleoside diphosphate kinase B	NME2	Homo sapiens	130.86	34.21	4	38
P60842	Eukaryotic initiation factor 4A-I	EIF4A1	Homo sapiens	119.34	13.30	4	38
P05141	ADP/ATP translocase 2	SLC25A5	Homo sapiens	107.37	28.19	8	38
P04080	Cystatin-B	CSTB	Homo sapiens	150.19	45.92	3	37
P62158	Calmodulin	CALM1	Homo sapiens	149.55	29.53	4	37
P52209	6-phosphogluconate dehydrogenase,	PGD	Homo sapiens	146.06	18.22	6	37
	decarboxylating
P01033	Metalloproteinase inhibitor 1	TIMP1	Homo sapiens	145.27	28.02	4	37
Q86VP6	Cullin-associated NEDD8-dissociated protein 1	CAND1	Homo sapiens	126.64	9.02	8	37
Q07020	60S ribosomal protein L18	RPL18	Homo sapiens	112.50	30.85	5	37
P60033	CD81 antigen	CD81	Homo sapiens	193.33	25.00	3	36
P07858	Cathepsin B	CTSB	Homo sapiens	176.56	23.60	5	36
Q9Y678	Coatomer subunit gamma-1	COPG1	Homo sapiens	151.53	10.41	6	36
P52907	F-actin-capping protein subunit alpha-1	ALB	Homo sapiens	138.39	34.62	6	36
P19105	Myosin regulatory light chain 12A	CAPZA1	Homo sapiens	134.09	39.18	5	36
P21589	5′-nucleotidase	MYL12A	Homo sapiens	125.92	18.82	8	36
Q96KK5	Histone H2A type 1-H	NT5E	Homo sapiens	123.16	27.34	3	36
P50995	Annexin A11	HIST1H2AH	Homo sapiens	121.26	15.84	6	36
Q969G5	Protein kinase C delta-binding protein	ANXA11	Homo sapiens	119.53	25.29	6	36
P52565	Rho GDP-dissociation inhibitor 1	PRKCDBP	Homo sapiens	117.26	21.08	4	36
P45880	Voltage-dependent anion-selective channel	ARHGDIA	Homo sapiens	111.78	18.03	4	36
	protein 2
Q96CX2	BTB/POZ domain-containing protein KCTD12	VDAC2	Homo sapiens	111.07	14.77	4	36
P02770	Serum albumin	KCTD12	Rattus norvegicus	139.43	5.92	3	36
P62979	Ubiquitin-40S ribosomal protein S27a	RPS27A	Homo sapiens	119.99	33.97	4	35
P40925	Malate dehydrogenase, cytoplasmic	MDH1	Homo sapiens	115.14	25.15	6	35
P05556	Integrin beta-1	ITGB1	Homo sapiens	114.15	9.77	6	35
P50395	Rab GDP dissociation inhibitor beta	FMOD	Homo sapiens	126.73	21.35	6	34
P61247	40S ribosomal protein S3a	GDI2	Homo sapiens	116.67	18.18	4	34
P07384	Calpain-1 catalytic subunit	RPS3A	Homo sapiens	114.52	7.56	4	34
P10599	Thioredoxin	CAPN1	Homo sapiens	111.79	22.86	2	34
P36955	Pigment epithelium-derived factor	TXN	Homo sapiens	111.21	11.96	4	34
P07910	Heterogeneous nuclear ribonucleoproteins C1/C2	SERPINF1	Homo sapiens	96.99	13.07	4	34
P50609	Fibromodulin	HNRNPC	Rattus norvegicus	129.65	11.17	3	34
P62906	60S ribosomal protein L10a	RPL10A	Homo sapiens	130.04	25.81	4	33
P62888	60S ribosomal protein L30	RPL30	Homo sapiens	113.47	34.78	3	33
P51884	Lumican	LUM	Homo sapiens	107.66	24.56	6	33
Q16658	Fascin	FSCN1	Homo sapiens	128.33	17.04	7	32
Q01813	6-phosphofructokinase type C	PFKP	Homo sapiens	126.94	10.46	5	32
P69905	Hemoglobin subunit alpha	HBA1;	Homo sapiens	124.85	28.17	3	32
P04216	Thy-1 membrane glycoprotein	THY1	Homo sapiens	121.63	24.22	3	32
P14314	Glucosidase 2 subunit beta	PRKCSH	Homo sapiens	120.87	9.85	4	32
P23219	Prostaglandin G/H synthase 1	PTGS1	Homo sapiens	120.15	12.85	5	32
Q15293	Reticulocalbin-1	RCN1	Homo sapiens	118.65	22.36	4	32
P04899	Guanine nucleotide-binding protein G(i)	GNAI2	Homo sapiens	117.69	20.28	5	32
	subunit alpha-2
P78417	Glutathione S-transferase omega-1	GSTO1	Homo sapiens	97.78	19.92	4	32
P55209	Nucleosome assembly protein 1-like 1	NAP1L1	Homo sapiens	125.54	14.58	4	31
P62701	40S ribosomal protein S4, X isoform	RPS4X	Homo sapiens	116.91	25.10	5	31
P12956	X-ray repair cross-complementing protein 6	XRCC6	Homo sapiens	94.21	12.32	6	31
Q16851	UTP--glucose-1-phosphate uridylyltransferase	UGP2	Homo sapiens	154.66	12.60	4	30
P06744	Glucose-6-phosphate isomerase	GPI	Homo sapiens	127.61	17.74	5	30
Q9Y696	Chloride intracellular channel protein 4	CLIC4	Homo sapiens	122.96	40.32	7	30
P30153	Serine/threonine-protein phosphatase 2A 65	PPP2R1A	Homo sapiens	116.53	19.52	7	30
	kDa regulatory subunit A alpha isoform
Q9NQC3	Reticulon-4	RTN4	Homo sapiens	105.15	9.65	6	30
P34932	Heat shock 70 kDa protein 4	HSPA4	Homo sapiens	97.65	12.86	7	30
Q15366	Poly(rC)-binding protein 2	PCBP2	Homo sapiens	96.66	14.79	4	30
P83731	60S ribosomal protein L24	DCN	Homo sapiens	90.70	20.38	3	30
P62249	40S ribosomal protein S16	RPL24	Homo sapiens	85.51	22.60	3	30
Q01129	Decorin	RPS16	Rattus norvegicus	90.83	22.60	7	30
P07108	Acyl-CoA-binding protein	DBI	Homo sapiens	129.14	50.57	3	29
P29373	Cellular retinoic acid-binding protein 2	CRABP2	Homo sapiens	126.14	34.78	4	29
P09651	Heterogeneous nuclear ribonucleoprotein A1	HNRNPA1	Homo sapiens	110.16	30.11	8	29
P16070	CD44 antigen	CD44	Homo sapiens	108.92	3.77	2	29
O43390	Heterogeneous nuclear ribonucleoprotein R	HNRNPR	Homo sapiens	103.11	9.79	5	29
P51991	Heterogeneous nuclear ribonucleoprotein A3	HNRNPA3	Homo sapiens	99.86	17.20	5	29
P39019	40S ribosomal protein S19	RPS19	Homo sapiens	84.77	35.86	7	29
P07686	Beta-hexosaminidase subunit beta	HEXB	Homo sapiens	78.39	7.55	4	29
Q04446	1,4-alpha-glucan-branching enzyme	GBE1	Homo sapiens	126.14	16.95	7	28
P62829	60S ribosomal protein L23	RPL23	Homo sapiens	103.56	25.00	2	28
P10909	Clusterin	CLU	Homo sapiens	97.80	14.03	4	28
P55786	Puromycin-sensitive aminopeptidase	C3	Homo sapiens	86.55	5.98	5	28
P00505	Aspartate aminotransferase, mitochondrial	NPEPPS	Homo sapiens	84.77	11.40	4	28
P01026	Complement C3 [Cleaved into: Complement	GOT2	Rattus norvegicus	90.69	1.74	2	28
	C3 beta chain; Complement C3 alpha chain]
P35527	Keratin, type I cytoskeletal 9	KRT9	Homo sapiens	117.56	25.36	10	27
P13667	Protein disulfide-isomerase A4	PDIA4	Homo sapiens	109.73	12.25	5	27
P50991	T-complex protein 1 subunit delta	CCT4	Homo sapiens	108.02	10.95	4	27
Q00839	Heterogeneous nuclear ribonucleoprotein U	HNRNPU	Homo sapiens	96.33	12.97	6	27
P30044	Peroxiredoxin-5, mitochondrial	PRDX5	Homo sapiens	89.27	26.17	4	27
P07602	Proactivator polypeptide [Cleaved into:	PSAP	Homo sapiens	78.87	10.31	5	27
	Saposin-A]
P20810	Calpastatin	CAST	Homo sapiens	119.50	10.88	4	26
P17931	Galectin-3	LGALS3	Homo sapiens	98.39	15.20	3	26
P22105	Tenascin-X	TNXB	Homo sapiens	96.39	4.17	8	26
P60903	Protein S100-A10	S100A10	Homo sapiens	95.99	17.53	2	26
P62277	40S ribosomal protein S13	RPS13	Homo sapiens	93.59	17.88	2	26
P23634	Plasma membrane calcium-transporting	ATP2B4	Homo sapiens	93.32	6.29	5	26
	ATPase 4
P32119	Peroxiredoxin-2	PRDX2	Homo sapiens	89.25	14.65	3	26
P46782	40S ribosomal protein S5 [Cleaved into: 40S	RPS5	Homo sapiens	87.53	13.73	2	26
	ribosomal protein S5, N-terminally processed]
P19823	Inter-alpha-trypsin inhibitor heavy chain H2	ITIH2	Homo sapiens	86.44	3.91	3	26
Q14974	Importin subunit beta-1	KPNB1	Homo sapiens	82.63	13.70	8	26
P53618	Coatomer subunit beta	COPB1	Homo sapiens	81.46	12.38	8	26
P31949	Protein S100-A11	S100A11	Homo sapiens	73.32	25.71	3	26
P59998	Actin-related protein 2/3 complex subunit 4	ARPC4	Homo sapiens	71.67	17.88	3	26
Q7KZF4	Staphylococcal nuclease domain-containing	SND1	Homo sapiens	100.24	8.35	5	25
	protein 1
P18124	60S ribosomal protein L7	RPL7	Homo sapiens	89.60	16.13	3	25
Q12797	Aspartyl/asparaginyl beta-hydroxylase	ASPH	Homo sapiens	83.42	10.55	5	25
Q8NBS9	Thioredoxin domain-containing protein 5	TXNDC5	Homo sapiens	111.81	9.26	3	24
P47756	F-actin-capping protein subunit beta	CAPZB	Homo sapiens	97.32	17.33	3	24
P47755	F-actin-capping protein subunit alpha-2	CAPZA2	Homo sapiens	93.19	28.67	5	24
P06748	Nucleophosmin	NPM1	Homo sapiens	88.64	12.24	3	24
P15531	Nucleoside diphosphate kinase A	NME1	Homo sapiens	82.11	33.55	4	24
P01024	Complement C3	C3	Homo sapiens	79.98	2.47	3	24
P53621	Coatomer subunit alpha	COPA	Homo sapiens	76.36	7.76	7	24
O75915	PRA1 family protein 3	ARL6IP5	Homo sapiens	71.25	15.96	2	24
Q15582	Transforming growth factor-beta-induced	TGFBI	Homo sapiens	71.03	7.91	4	24
	protein ig-h3
P60981	Destrin	DSTN	Homo sapiens	67.90	25.45	4	24
P02774	Vitamin D-binding protein	GC	Homo sapiens	116.41	6.33	2	23
P62263	40S ribosomal protein S14	RPS14	Homo sapiens	90.99	27.81	3	23
P63010	AP-2 complex subunit beta	AP2B1	Homo sapiens	87.06	8.54	5	23
O43399	Tumor protein D54	TPD52L2	Homo sapiens	85.22	24.27	3	23
P62826	GTP-binding nuclear protein Ran	RAN	Homo sapiens	82.88	22.69	4	23
P61106	Ras-related protein Rab-14	RAB14	Homo sapiens	82.68	25.58	3	23
P08134	Rho-related GTP-binding protein RhoC	RHOC	Homo sapiens	78.51	26.94	4	23
P17980	26S protease regulatory subunit 6A	PSMC3	Homo sapiens	76.14	17.08	5	23
P62879	Guanine nucleotide-binding protein	GNB2	Homo sapiens	69.24	15.59	5	23
	G(I)/G(S)/G(T) subunit beta-2
Q13838	Spliceosome RNA helicase DDX39B	DDX39B	Homo sapiens	68.43	10.51	4	23
P02533	Keratin, type I cytoskeletal 14	KRT14	Homo sapiens	64.86	17.80	9	23
O75390	Citrate synthase, mitochondrial	CS	Homo sapiens	63.78	10.09	4	23
Q92743	Serine protease HTRA1	HTRA1	Homo sapiens	61.31	8.75	4	23
P62269	40S ribosomal protein S18	RPS18	Homo sapiens	60.45	16.45	3	23
Q07021	Complement component 1 Q subcomponent-	C1QBP	Homo sapiens	94.64	12.06	3	22
	binding protein, mitochondrial
P62244	40S ribosomal protein S15a	RPS15A	Homo sapiens	93.74	24.62	3	22
P13693	Translationally-controlled tumor protein	TPT1	Homo sapiens	82.09	12.21	2	22
O60506	Heterogeneous nuclear ribonucleoprotein Q	SYNCRIP	Homo sapiens	80.19	13.16	6	22
Q00325	Phosphate carrier protein, mitochondrial	SLC25A3	Homo sapiens	75.91	14.64	4	22
P78527	DNA-dependent protein kinase catalytic	PRKDC	Homo sapiens	74.42	2.06	6	22
	subunit
P15880	40S ribosomal protein S2	RPS2	Homo sapiens	71.50	16.04	4	22
Q96TA1	Niban-like protein 1	FAM129B	Homo sapiens	70.01	9.79	5	22
P62857	40S ribosomal protein S28	RPS28	Homo sapiens	69.54	33.33	2	22
P02788	Lactotransferrin	LTF	Homo sapiens	61.38	4.23	3	22
P62942	Peptidyl-prolyl cis-trans isomerase FKBP1A	FKBP1A	Homo sapiens	87.52	29.63	3	21
P15559	NAD(P)H dehydrogenase [quinone] 1	NQO1	Homo sapiens	81.73	13.50	3	21
O00159	Unconventional myosin-Ic	MYO1C	Homo sapiens	69.32	8.18	6	21
Q9BSJ8	Extended synaptotagmin-1	ESYT1	Homo sapiens	66.23	8.70	7	21
P02787	Serotransferrin	TF	Homo sapiens	66.03	16.76	8	21
Q8WUM4	Programmed cell death 6-interacting protein	PDCD6IP	Homo sapiens	64.27	7.49	4	21
P17987	T-complex protein 1 subunit alpha	TCP1	Homo sapiens	61.00	10.79	5	21
P16403	Histone H1.2	HIST1H1C	Homo sapiens	57.53	15.49	3	21
P05366	60S acidic ribosomal protein P1	RPLP1	Homo sapiens	110.45	57.02	3	20
P68036	Ubiquitin-conjugating enzyme E2 L3	UBE2L3	Homo sapiens	92.04	35.71	3	20
P25398	40S ribosomal protein S12	RPS12	Homo sapiens	84.49	31.82	3	20
P31943	Heterogeneous nuclear ribonucleoprotein H	HNRNPH1	Homo sapiens	76.64	10.02	3	20
Q99623	Prohibitin-2	PHB2	Homo sapiens	76.40	16.72	4	20
Q07954	Prolow-density lipoprotein receptor-related protein 1	LRP1	Homo sapiens	75.28	2.13	6	20
P12955	Xaa-Pro dipeptidase	PEPD	Homo sapiens	74.39	8.32	3	20
O95782	AP-2 complex subunit alpha-1	AP2A1	Homo sapiens	71.27	5.94	4	20
P56134	ATP synthase subunit f, mitochondrial	ATP5J2	Homo sapiens	61.83	25.53	2	20
P26447	Protein S100-A4	S100A4	Homo sapiens	61.18	28.71	3	20
P06779	Keratin, type I cytoskeletal 16	KRT16	Homo sapiens	54.25	15.01	8	20
P78371	T-complex protein 1 subunit beta	CCT2	Homo sapiens	81.92	17.01	6	19
P62140	Serine/threonine-protein phosphatase PP1-	PPP1CB	Homo sapiens	67.52	21.41	6	19
	beta catalytic subunit
P29692	Elongation factor 1-delta	EEF1D	Homo sapiens	61.11	17.08	3	19
P46783	40S ribosomal protein S10	RPS10	Homo sapiens	58.94	14.55	2	19
P19338	Nucleolin	NCL	Homo sapiens	58.45	9.30	5	19
P13797	Plastin-3	PLS3	Homo sapiens	56.74	16.67	8	19
Q6NXT2	Histone H3.3C	H3F3C	Homo sapiens	54.28	11.85	2	19
P35606	Coatomer subunit beta′	COPB2	Homo sapiens	52.01	10.82	7	19
P46781	40S ribosomal protein S9	RPS9	Homo sapiens	50.85	17.53	4	19
P26599	Polypyrimidine tract-binding protein 1	PTBP1	Homo sapiens	75.12	15.63	4	18
P31939	Bifunctional purine biosynthesis protein PURH	ATIC	Homo sapiens	68.66	8.78	4	18
	[Includes:
	Phosphoribosylaminoimidazolecarboxamide
	formyltransferase]
P49755	Transmembrane emp24 domain-containing	TMED10	Homo sapiens	68.15	12.79	2	18
	protein 10
P49411	Elongation factor Tu, mitochondrial	TUFM	Homo sapiens	66.68	18.58	5	18
P14550	Alcohol dehydrogenase [NADP(+)]	AKR1A1	Homo sapiens	63.84	13.54	4	18
Q14195	Dihydropyrimidinase-related protein 3	DPYSL3	Homo sapiens	62.54	21.58	8	18
Q15436	Protein transport protein Sec23A	SEC23A	Homo sapiens	60.90	5.75	3	18
Q9Y2Q3	Glutathione S-transferase kappa 1	GSTK1	Homo sapiens	59.42	23.89	4	18
Q14108	Lysosome membrane protein 2	SCARB2	Homo sapiens	58.13	9.00	3	18
P48444	Coatomer subunit delta	ARCN1	Homo sapiens	56.94	6.46	3	18
P41250	Glycine-tRNA ligase	GARS	Homo sapiens	55.95	7.71	4	18
Q04760	Lactoylglutathione lyase	GLO1	Homo sapiens	54.07	19.57	3	18
P50914	60S ribosomal protein L14	RPL14	Homo sapiens	53.56	15.35	3	18
P35232	Prohibitin	PHB	Homo sapiens	53.10	12.13	3	18
P11940	Polyadenylate-binding protein 1	PABPC1	Homo sapiens	49.49	7.55	4	18
Q9H4M9	EH domain-containing protein 1	EHD1	Homo sapiens	79.67	12.36	4	17
Q15185	Prostaglandin E synthase 3	PTGES3	Homo sapiens	73.37	24.38	3	17
Q13492	Phosphatidylinositol-binding clathrin assembly protein	PICALM	Homo sapiens	61.52	4.91	2	17
P60953	Cell division control protein 42 homolog	CDC42	Homo sapiens	61.37	20.42	3	17
P54920	Alpha-soluble NSF attachment protein	NAPA	Homo sapiens	58.25	17.97	4	17
O00571	ATP-dependent RNA helicase DDX3X	DDX3X	Homo sapiens	55.60	9.67	5	17
Q13162	Peroxiredoxin-4	PRDX4	Homo sapiens	48.19	11.81	3	17
P61160	Actin-related protein 2	ACTR2	Homo sapiens	44.56	17.77	6	17
P30048	Thioredoxin-dependent peroxide reductase,	PRDX3	Homo sapiens	79.84	18.75	2	16
	mitochondrial
P62280	40S ribosomal protein S11	RPS11	Homo sapiens	67.34	16.46	2	16
Q8IUX7	Adipocyte enhancer-binding protein 1	AEBP1	Homo sapiens	65.82	5.96	4	16
P55084	Trifunctional enzyme subunit beta,	HADHB	Homo sapiens	63.53	9.28	3	16
	mitochondrial
Q07960	Rho GTPase-activating protein 1	ARHGAP1	Homo sapiens	62.30	14.81	4	16
P98160	Basement membrane-specific heparan sulfate	HSPG2	Homo sapiens	61.92	1.80	5	16
	proteoglycan core protein
P54709	Sodium/potassium-transporting ATPase	ATP1B3	Homo sapiens	58.07	17.20	3	16
	subunit beta-3
P37837	Transaldolase	TALDO1	Homo sapiens	53.35	7.12	2	16
P61224	Ras-related protein Rap-1b	RAP1B	Homo sapiens	49.98	20.65	3	16
Q14103	Heterogeneous nuclear ribonucleoprotein D0	HNRNPD	Homo sapiens	48.34	6.76	2	16
P14866	Heterogeneous nuclear ribonucleoprotein L	HNRNPL	Homo sapiens	71.48	8.66	2	15
P51636	Caveolin-2	CAV2	Homo sapiens	65.54	29.63	3	15
O15511	Actin-related protein 2/3 complex subunit 5	ARPC5	Homo sapiens	61.90	20.53	2	15
O43242	26S proteasome non-ATPase regulatory	PSMD3	Homo sapiens	61.84	5.62	2	15
	subunit 3
Q08211	ATP-dependent RNA helicase A	DHX9	Homo sapiens	61.31	5.04	4	15
Q15181	Inorganic pyrophosphatase	PPA1	Homo sapiens	60.87	23.18	4	15
P46777	60S ribosomal protein L5	RPL5	Homo sapiens	60.47	19.87	4	15
P61604	10 kDa heat shock protein, mitochondrial	HSPE1	Homo sapiens	58.06	33.33	3	15
P10620	Microsomal glutathione S-transferase 1	MGST1	Homo sapiens	57.64	19.35	2	15
Q13765	Nascent polypeptide-associated complex	NACA	Homo sapiens	56.71	18.60	3	15
	subunit alpha
P17858	6-phosphofructokinase, liver type	PFKL	Homo sapiens	55.45	6.54	3	15
Q9P2E9	Ribosome-binding protein 1	RRBP1	Homo sapiens	52.97	6.60	6	15
P16615	Sarcoplasmic/endoplasmic reticulum calcium	ATP2A2	Homo sapiens	52.48	5.37	4	15
	ATPase 2
P15121	Aldose reductase	AKR1B1	Homo sapiens	51.96	24.37	4	15
Q8NC51	Plasminogen activator inhibitor 1 RNA-binding	SERBP1	Homo sapiens	49.13	18.14	5	15
	protein
P51659	Peroxisomal multifunctional enzyme type 2	HSD17B4	Homo sapiens	48.08	11.01	5	15
P10155	60 kDa SS-A/Ro ribonucleoprotein	TROVE2	Homo sapiens	48.07	4.46	2	15
O94979	Protein transport protein Sec31A	SEC31A	Homo sapiens	47.81	4.51	4	15
P46778	60S ribosomal protein L21	RPL21	Homo sapiens	46.39	27.50	3	15
P78539	Sushi repeat-containing protein SRPX	SRPX	Homo sapiens	45.56	7.76	3	15
P51148	Ras-related protein Rab-5C	RAB5C	Homo sapiens	44.79	23.15	4	15
Q14647	LIM and SH3 domain protein 1	LASP1	Homo sapiens	44.78	14.18	3	15
Q9ULV4	Coronin-1C	CORO1C	Homo sapiens	44.37	8.23	3	15
O43776	Asparagine--tRNA ligase, cytoplasmic	NARS	Homo sapiens	43.41	8.94	4	15
Q14152	Eukaryotic translation initiation factor 3 subunit	EIF3A	Homo sapiens	42.47	3.47	4	15
	A
P35268	60S ribosomal protein L22	RPL22	Homo sapiens	41.79	18.75	2	15
P37235	Hippocalcin-like protein 1	HPCAL1	Homo sapiens	41.04	23.83	4	15
P00441	Superoxide dismutase [Cu—Zn]	SOD1	Homo sapiens	75.69	32.47	4	14
Q99460	26S proteasome non-ATPase regulatory	PSMD1	Homo sapiens	69.67	5.46	3	14
	subunit 1
Q08431	Lactadherin	MFGE8	Homo sapiens	67.71	8.53	2	14
O95373	Importin-7	IPO7	Homo sapiens	61.22	4.53	3	14
Q16181	Septin-7	41889	Homo sapiens	55.03	13.50	4	14
Q13200	26S proteasome non-ATPase regulatory subunit 2	PSMD2	Homo sapiens	53.05	5.18	3	14
Q9Y265	RuvB-like 1	RUVBL1	Homo sapiens	50.48	15.79	5	14
Q9UBG0	C-type mannose receptor 2	MRC2	Homo sapiens	48.78	2.77	3	14
P51571	Translocon-associated protein subunit delta	SSR4	Homo sapiens	44.44	24.86	3	14
Q9H8H3	Methyltransferase-like protein 7A	METTL7A	Homo sapiens	42.92	12.30	2	14
Q16698	2,4-dienoyl-CoA reductase, mitochondrial	DECR1	Homo sapiens	40.03	13.73	3	14
Q9UHD8	Septin-9	41891	Homo sapiens	39.86	11.60	5	14
Q9NVA2	Septin-11	41893	Homo sapiens	38.96	11.19	4	14
Q99829	Copine-1	CPNE1	Homo sapiens	38.95	6.33	3	14
Q92499	ATP-dependent RNA helicase DDX1	DDX1	Homo sapiens	64.96	7.43	3	13
P07099	Epoxide hydrolase 1	EPHX1	Homo sapiens	62.32	10.77	3	13
Q99497	Protein DJ-1	PARK7	Homo sapiens	58.25	29.63	3	13
P40227	T-complex protein 1 subunit zeta	CCT6A	Homo sapiens	57.05	6.97	2	13
P28838	Cytosol aminopeptidase	LAP3	Homo sapiens	51.95	12.33	4	13
Q9UBI6	Guanine nucleotide-binding protein	GNG12	Homo sapiens	50.08	31.94	2	13
	G(I)/G(S)/G(O) subunit gamma-12
O14818	Proteasome subunit alpha type-7	PSMA7	Homo sapiens	48.17	20.56	3	13
P19367	Hexokinase-1	HK1	Homo sapiens	45.34	5.89	4	13
P39023	60S ribosomal protein L3	RPL3	Homo sapiens	44.04	12.66	3	13
Q6YHK3	CD109 antigen	CD109	Homo sapiens	42.37	7.47	7	13
P46776	60S ribosomal protein L27a	RPL27A	Homo sapiens	39.08	14.19	2	13
Q99584	Protein S100-A13	S100A13	Homo sapiens	39.08	23.47	2	13
Q15636	Vesicle-associated membrane protein 3	VAMP3	Homo sapiens	38.92	33.00	2	13
Q92688	Acidic leucine-rich nuclear phosphoprotein 32	ANP32B	Homo sapiens	38.66	15.94	3	13
	family member B
Q9Y6N5	Sulfide quinone oxidoreductase, mitochondrial	SQRDL	Homo sapiens	37.33	10.00	4	13
P62318	Small nuclear ribonucleoprotein Sm D3	SNRPD3	Homo sapiens	34.78	15.08	2	13
P01008	Antithrombin-III	SERPINC1	Homo sapiens	45.87	4.31	2	12
P28066	Proteasome subunit alpha type-5	PSMA5	Homo sapiens	43.47	22.41	3	12
O75874	Isocitrate dehydrogenase [NADP] cytoplasmic	IDH1	Homo sapiens	41.65	10.14	3	12
P48047	ATP synthase subunit O, mitochondrial	ATP5O	Homo sapiens	40.13	29.11	4	12
Q9BWM7	Sideroflexin-3	SFXN3	Homo sapiens	37.95	8.62	2	12
P61088	Ubiquitin-conjugating enzyme E2 N	UBE2N	Homo sapiens	36.77	27.63	3	12
Q05682	Caldesmon	CALD1	Homo sapiens	36.05	5.30	4	12
P38159	RNA-binding motif protein, X chromosome	RBMX	Homo sapiens	35.98	8.95	3	12
O15260	Surfeit locus protein 4	SURF4	Homo sapiens	35.84	8.55	2	12
P30493	HLA class I histocompatibility antigen, B-55	HLA-B	Homo sapiens	34.77	14.64	4	12
	alpha chain
P62851	40S ribosomal protein S25	RPS25	Homo sapiens	34.69	16.00	3	12
P27816	Microtubule-associated protein 4	MAP4	Homo sapiens	34.36	4.69	4	12
P61353	60S ribosomal protein L27	RPL27	Homo sapiens	33.55	27.94	3	12
P52272	Heterogeneous nuclear ribonucleoprotein M	HNRNPM	Homo sapiens	33.53	7.67	4	12
Q15417	Calponin-3	CNN3	Homo sapiens	33.42	10.64	3	12
P49773	Histidine triad nucleotide-binding protein 1	HINT1	Homo sapiens	69.01	34.92	2	11
Q15121	Astrocytic phosphoprotein PEA-15	PEA15	Homo sapiens	52.21	27.69	3	11
Q12905	Interleukin enhancer-binding factor 2	ILF2	Homo sapiens	43.95	8.72	2	11
P68402	Platelet-activating factor acetylhydrolase IB subunit beta	PAFAH1B2	Homo sapiens	43.27	12.23	2	11
P08253	72 kDa type IV collagenase	MMP2	Homo sapiens	42.46	10.91	4	11
P62333	26S protease regulatory subunit 10B	PSMC6	Homo sapiens	41.19	14.91	4	11
P36957	Dihydrolipoyllysine-residue	DLST	Homo sapiens	40.04	10.15	3	11
	succinyltransferase component of 2-
	oxoglutarate dehydrogenase complex,
	mitochondrial
P61313	60S ribosomal protein L15	RPL15	Homo sapiens	38.61	12.75	2	11
P04632	Calpain small subunit 1	CAPNS1	Homo sapiens	36.46	16.42	3	11
P11047	Laminin subunit gamma-1	LAMC1	Homo sapiens	36.41	1.74	2	11
P08195	4F2 cell-surface antigen heavy chain	SLC3A2	Homo sapiens	34.57	4.13	2	11
Q01105	Protein SET	SET	Homo sapiens	34.28	12.07	3	11
Q9Y277	Voltage-dependent anion-selective channel protein 3	VDAC3	Homo sapiens	34.15	19.08	4	11
P62753	40S ribosomal protein S6	RPS6	Homo sapiens	31.26	10.84	2	11
O14979	Heterogeneous nuclear ribonucleoprotein D-	HNRNPDL	Homo sapiens	31.19	9.76	3	11
	like
P30040	Endoplasmic reticulum resident protein 29	ERP29	Homo sapiens	29.37	8.43	2	11
O00410	Importin-5	IPO5	Homo sapiens	46.49	6.47	4	10
Q8IWE2	Protein NOXP20	FAM114A1	Homo sapiens	45.84	5.68	2	10
O14579	Coatomer subunit epsilon	COPE	Homo sapiens	44.19	6.82	2	10
O00154	Cytosolic acyl coenzyme A thioester hydrolase	ACOT7	Homo sapiens	41.44	12.37	3	10
Q9Y3F4	Serine-threonine kinase receptor-associated	STRAP	Homo sapiens	41.18	8.86	2	10
	protein
P09429	High mobility group protein B1	HMGB1	Homo sapiens	39.56	27.44	4	10
O15144	Actin-related protein 2/3 complex subunit 2	ARPC2	Homo sapiens	36.55	18.00	3	10
Q96CW1	AP-2 complex subunit mu	AP2M1	Homo sapiens	32.81	12.87	4	10
PB4103	Serine/arginine-rich splicing factor 3	SRSF3	Homo sapiens	31.49	14.02	2	10
O60763	General vesicular transport factor p115	USO1	Homo sapiens	31.07	5.41	4	10
P40429	60S ribosomal protein L13a	RPL13A	Homo sapiens	25.35	8.87	2	10
Q6DD88	Atlastin-3	ATL3	Homo sapiens	40.84	12.38	4	9
P09622	Dihydrolipoyl dehydrogenase, mitochondrial	DLD	Homo sapiens	40.56	6.48	2	9
Q9BWD1	Acetyl-CoA acetyltransferase, cytosolic	ACAT2	Homo sapiens	37.63	14.61	3	9
Q00341	Vigilin	HDLBP	Homo sapiens	37.29	4.10	3	9
Q99798	Aconitate hydratase, mitochondrial	ACO2	Homo sapiens	36.61	4.49	2	9
Q02878	60S ribosomal protein L6	RPL6	Homo sapiens	34.78	15.63	3	9
Q15008	26S proteasome non-ATPase regulatory	PSMD6	Homo sapiens	34.04	7.20	2	9
	subunit 6
P21266	Glutathione S-transferase Mu 3	GSTM3	Homo sapiens	31.19	17.33	3	9
P10301	Ras-related protein R-Ras	RRAS	Homo sapiens	29.36	12.84	2	9
Q06323	Proteasome activator complex subunit 1	PSME1	Homo sapiens	26.97	23.29	4	9
Q13557	Calcium/calmodulin-dependent protein kinase	CAMK2D	Homo sapiens	23.13	8.62	3	9
	type II subunit delta
P21964	Catechol O-methyltransferase	COMT	Homo sapiens	34.16	8.49	2	8
P63167	Dynein light chain 1, cytoplasmic	DYNLL1	Homo sapiens	33.21	37.08	2	8
Q02952	A-kinase anchor protein 12	AKAP12	Homo sapiens	32.58	3.42	3	8
P16278	Beta-galactosidase	GLB1	Homo sapiens	29.46	3.69	2	8
P62081	40S ribosomal protein S7	RPS7	Homo sapiens	27.65	17.53	2	8
P31937	3-hydroxyisobutyrate dehydrogenase,	HIBADH	Homo sapiens	27.07	7.14	2	8
	mitochondrial
Q96QK1	Vacuolar protein sorting-associated protein 35	VPS35	Homo sapiens	26.95	2.89	2	8
P33176	Kinesin-1 heavy chain	KIF5B	Homo sapiens	26.86	2.80	2	8
P61019	Ras-related protein Rab-2A	RAB2A	Homo sapiens	26.44	16.87	3	8
O00231	26S proteasome non-ATPase regulatory	PSMD11	Homo sapiens	25.87	8.77	3	8
	subunit 11
P26373	60S ribosomal protein L13	RPL13	Homo sapiens	25.81	10.90	2	8
Q13724	Mannosyl-oligosaccharide glucosidase	MOGS	Homo sapiens	25.51	3.58	2	8
O95816	BAG family molecular chaperone regulator 2	BAG2	Homo sapiens	24.67	13.27	2	8
P06703	Protein S100-A6	S100A6	Homo sapiens	24.35	37.78	2	8
P34897	Serine hydroxymethyltransferase, mitochondrial	SHMT2	Homo sapiens	24.35	10.32	4	8
P17844	Probable ATP-dependent RNA helicase DDX5	DDX5	Homo sapiens	24.22	5.86	3	8
O94905	Erlin-2	ERLIN2	Homo sapiens	23.59	6.49	2	8
P08648	Integrin alpha-5	ITGA5	Homo sapiens	23.55	5.62	4	8
P62917	60S ribosomal protein L8	RPL8	Homo sapiens	22.51	10.51	2	8
P01023	Alpha-2-macroglobulin	A2M	Homo sapiens	20.91	2.04	3	8
P26022	Pentraxin-related protein PTX3	PTX3	Homo sapiens	20.28	13.12	4	8
P07814	Bifunctional glutamate/proline--tRNA ligase	EPRS	Homo sapiens	34.10	2.18	2	7
Q6IBS0	Twinfilin-2	TWF2	Homo sapiens	29.62	9.74	2	7
P49257	Protein ERGIC-53	LMAN1	Homo sapiens	28.55	10.39	2	7
O75368	SH3 domain-binding glutamic acid-rich-like	SH3BGRL	Homo sapiens	26.17	26.32	2	7
	protein
Q07666	KH domain-containing, RNA-binding, signal	KHDRBS1	Homo sapiens	24.96	7.67	2	7
	transduction-associated protein 1
P46977	Dolichyl-diphosphooligosaccharide--protein	STT3A	Homo sapiens	24.54	3.69	2	7
	glycosyltransferase subunit STT3A
Q7L2H7	Eukaryotic translation initiation factor 3 subunit M	EIF3M	Homo sapiens	24.21	6.95	2	7
Q15847	Adipogenesis regulatory factor	ADIRF	Homo sapiens	23.65	59.21	2	7
P48643	T-complex protein 1 subunit epsilon	CCT5	Homo sapiens	23.35	11.83	4	7
P12268	Inosine-5′-monophosphate dehydrogenase 2	IMPDH2	Homo sapiens	23.35	9.73	3	7
Q63ZY3	KN motif and ankyrin repeat domain-	KANK2	Homo sapiens	23.26	4.70	3	7
	containing protein 2
P22102	Trifunctional purine biosynthetic protein	GART	Homo sapiens	22.93	2.67	2	7
	adenosine-3 [Includes: Phosphoribosylamine--
	glycine ligase]
P49458	Signal recognition particle 9 kDa protein	SRP9	Homo sapiens	22.86	23.26	2	7
P05023	Sodium/potassium-transporting ATPase	ATP1A1	Homo sapiens	22.17	3.91	3	7
	subunit alpha-1
P02792	Ferritin light chain	FTL	Homo sapiens	22.12	26.86	3	7
P35613	Basigin	BSG	Homo sapiens	21.87	8.31	2	7
P18621	60S ribosomal protein L17	RPL17	Homo sapiens	21.02	17.93	3	7
P11177	Pyruvate dehydrogenase E1 component	PDHB	Homo sapiens	19.98	8.91	2	7
	subunit beta, mitochondrial
P62330	ADP-ribosylation factor 6	ARF6	Homo sapiens	19.84	12.57	2	7
Q99832	T-complex protein 1 subunit eta	CCT7	Homo sapiens	19.63	7.92	3	7
P35637	RNA-binding protein FUS	FUS	Homo sapiens	19.53	6.27	2	7
P42677	40S ribosomal protein S27	RPS27	Homo sapiens	19.53	25.00	2	7
P31948	Stress-induced-phosphoprotein 1	STIP1	Homo sapiens	19.50	7.73	3	7
P26640	Valine--tRNA ligase	VARS	Homo sapiens	18.39	1.98	2	7
P15586	N-acetylglucosamine-6-sulfatase	GNS	Homo sapiens	18.07	4.71	2	7
P62191	26S protease regulatory subunit 4	PSMC1	Homo sapiens	30.43	8.18	2	6
Q13423	NAD(P) transhydrogenase, mitochondrial	NNT	Homo sapiens	28.78	3.41	2	6
P09960	Leukotriene A-4 hydrolase	LTA4H	Homo sapiens	26.18	5.07	2	6
P22695	Cytochrome b-c1 complex subunit 2,	UQCRC2	Homo sapiens	24.46	10.82	3	6
	mitochondrial
P10619	Lysosomal protective protein	CTSA	Homo sapiens	22.01	5.00	2	6
P23381	Tryptophan--tRNA ligase, cytoplasmic	WARS	Homo sapiens	21.16	5.73	2	6
P02794	Ferritin heavy chain	FTH1	Homo sapiens	20.44	33.33	3	6
P55010	Eukaryotic translation initiation factor 5	EIF5	Homo sapiens	18.71	9.98	3	6
P35555	Fibrillin-1	FBN1	Homo sapiens	18.54	2.16	4	6
P39656	Dolichyl-diphosphooligosaccharide--protein	DDOST	Homo sapiens	18.03	7.89	3	6
	glycosyltransferase 48 kDa subunit
Q13425	Beta-2-syntrophin	SNTB2	Homo sapiens	17.22	3.89	2	6
O99961	Endophilin-A2	SH3GL1	Homo sapiens	17.15	8.97	2	6
P54136	Arginine--tRNA ligase, cytoplasmic	RARS	Homo sapiens	17.09	3.64	2	6
P20073	Annexin A7	ANXA7	Homo sapiens	15.98	8.20	3	6
O15113	Procollagen C-endopeptidase enhancer 1	PCOLCE	Homo sapiens	15.72	6.01	2	6
P30085	UMP-CMP kinase	CMPK1	Homo sapiens	24.26	16.84	2	5
Q16881	Thioredoxin reductase 1, cytoplasmic	TXNRD1	Homo sapiens	22.69	5.86	2	5
P00568	Adenylate kinase isoenzyme 1	AK1	Homo sapiens	19.57	19.07	2	5
P43243	Matrin-3	MATR3	Homo sapiens	18.67	6.02	3	5
O95292	Vesicle-associated membrane protein-	VAPB	Homo sapiens	16.59	10.70	2	5
	associated protein B/C
O43237	Cytoplasmic dynein 1 light intermediate chain	DYNC1LI2	Homo sapiens	16.24	5.08	2	5
	2
P23526	Adenosylhomocysteinase	AHCY	Homo sapiens	15.78	10.65	3	5
P13804	Electron transfer flavoprotein subunit alpha, mitochondrial	ETFA	Homo sapiens	15.40	8.71	2	5
P13010	X-ray repair cross-complementing protein 5	XRCC5	Homo sapiens	15.04	6.42	3	5
Q01995	Transgelin	TAGLN	Homo sapiens	15.00	12.44	2	5
P60900	Proteasome subunit alpha type-6	PSMA6	Homo sapiens	14.55	10.16	2	5
P07996	Thrombospondin-1	THBS1	Homo sapiens	14.55	1.97	2	5
Q9UEY8	Gamma-adducin	ADD3	Homo sapiens	14.49	3.54	2	5
O60488	Long-chain-fatty-acid-CoA ligase 4	ACSL4	Homo sapiens	13.17	4.36	2	5
Q15942	Zyxin	ZYX	Homo sapiens	22.91	6.64	2	4
O14980	Exportin-1	XPO1	Homo sapiens	22.59	4.20	2	4
O75131	Copine-3	CPNE3	Homo sapiens	18.51	6.33	2	4
Q99714	3-hydroxyacyl-CoA dehydrogenase type-2	HSD17B10	Homo sapiens	17.39	16.48	2	4
O94855	Protein transport protein Sec24D	SEC24D	Homo sapiens	16.72	2.91	2	4
P21291	Cysteine and glycine-rich protein 1	CSRP1	Homo sapiens	16.40	19.17	2	4
Q9BVK6	Transmembrane emp24 domain-containing	TMED9	Homo sapiens	15.78	14.47	2	4
	protein 9
Q13283	Ras GTPase-activating protein-binding protein 1	G3BP1	Homo sapiens	15.21	6.01	2	4
Q9NYL9	Tropomodulin-3	TMOD3	Homo sapiens	14.89	9.38	2	4
P62195	26S protease regulatory subunit 8	PSMC5	Homo sapiens	14.87	12.07	3	4
P55884	Eukaryotic translation initiation factor 3 subunit	EIF3B	Homo sapiens	14.81	4.67	2	4
	B
P33527	Multidrug resistance-associated protein 1	ABCC1	Homo sapiens	13.99	2.02	2	4
O15143	Actin-related protein 2/3 complex subunit 1B	ARPC1B	Homo sapiens	13.50	11.83	3	4
Q9Y262	Eukaryotic translation initiation factor 3 subunit	EIF3L	Homo sapiens	13.47	3.55	2	4
	L
Q02809	Procollagen-lysine, 2-oxoglutarate 5-	PLOD1	Homo sapiens	13.29	3.71	2	4
	dioxygenase 1
P23246	Splicing factor, proline- and glutamine-rich	SFPQ	Homo sapiens	13.13	6.22	3	4
P30046	D-dopachrome decarboxylase	DDT	Homo sapiens	12.61	19.49	2	4
Q9NQW7	Xaa-Pro aminopeptidase 1	XPNPEP1	Homo sapiens	12.18	4.82	2	4
Q9NR31	GTP-binding protein SAR1a	SAR1A	Homo sapiens	12.08	15.66	2	4
Q03252	Lamin-B2	LMNB2	Homo sapiens	11.81	5.67	3	4
P78344	Eukaryotic translation initiation factor 4	EIF4G2	Homo sapiens	11.24	2.32	2	4
	gamma 2
P41252	Isoleucine--tRNA ligase, cytoplasmic	IARS	Homo sapiens	11.24	2.69	3	4
Q7L576	Cytoplasmic FMR1-interacting protein 1	CYFIP1	Homo sapiens	11.23	1.84	2	4
P67809	Nuclease-sensitive element-binding protein 1	YBX1	Homo sapiens	10.94	11.11	2	4
P35611	Alpha-adducin	ADD1	Homo sapiens	10.23	4.21	2	4
Q9BR76	Coronin-1B	CORO1B	Homo sapiens	10.19	3.48	2	4
P27695	DNA-(apurinic or apyrimidinic site) lyase	APEX1	Homo sapiens	16.69	11.01	2	3
O15460	Prolyl 4-hydroxylase subunit alpha-2	P4HA2	Homo sapiens	14.16	6.17	2	3
Q96HE7	ERO1-like protein alpha	ERO1L	Homo sapiens	13.09	7.26	2	3
P61769	Beta-2-microglobulin [Cleaved into: Beta-2-	B2M	Homo sapiens	11.85	26.89	2	3
	microglobulin form pl 5.3]
P14854	Cytochrome c oxidase subunit 6B1	COX6B1	Homo sapiens	11.35	33.72	2	3
P00390	Glutathione reductase, mitochondrial	GSR	Homo sapiens	11.32	6.32	2	3
P13798	Acylamino-acid-releasing enzyme	APEH	Homo sapiens	10.86	4.78	2	3
Q08257	Quinone oxidoreductase	CRYZ	Homo sapiens	9.95	10.64	2	3
Q9NTK5	Obg-like ATPase 1	OLA1	Homo sapiens	9.88	7.32	2	3
Q13418	Integrin-linked protein kinase	ILK	Homo sapiens	9.86	8.63	3	3
P61009	Signal peptidase complex subunit 3	SPCS3	Homo sapiens	9.85	12.78	2	3
Q969H8	UPF0556 protein C19orf10	C19ORF10	Homo sapiens	9.71	15.03	2	3
P05455	Lupus La protein	SSB	Homo sapiens	9.57	6.37	2	3
P51858	Hepatoma-derived growth factor	HDGF	Homo sapiens	9.38	13.75	2	3
O00425	Insulin-like growth factor 2 mRNA-binding	IGF2BP3	Homo sapiens	8.88	5.18	2	3
	protein 3
P20042	Eukaryotic translation initiation factor 2 subunit	EIF2S2	Homo sapiens	8.84	8.11	2	3
	2
P62495	Eukaryotic peptide chain release factor subunit 1	ETF1	Homo sapiens	8.74	6.41	2	3
P11279	Lysosome-associated membrane glycoprotein 1	LAMP1	Homo sapiens	8.45	6.00	2	3
Q9NUQ9	Protein FAM49B	FAM49B	Homo sapiens	8.18	8.64	2	3
Q14247	Src substrate cortactin	CTTN	Homo sapiens	8.15	3.64	2	3
P27708	CAD protein [Includes: Glutamine-dependent	CAD	Homo sapiens	7.94	0.94	2	3
	carbamoyl-phosphate synthase]
Q9NSE4	Isoleucine--tRNA ligase, mitochondrial	IARS2	Homo sapiens	7.76	2.08	2	3
Q9H4A4	Aminopeptidase B	RNPEP	Homo sapiens	7.62	3.54	2	3
P62913	60S ribosomal protein L11	RPL11	Homo sapiens	7.39	12.92	2	3
P39060	Collagen alpha-1(XVIII) chain [Cleaved into:	COL18A1	Homo sapiens	11.40	2.85	2	2
	Endostatin]
P26368	Splicing factor U2AF 65 kDa subunit	U2AF2	Homo sapiens	8.61	6.11	2	2
Q687X5	Metalloreductase STEAP4	STEAP4	Homo sapiens	7.68	7.19	2	2
P31153	S-adenosylmethionine synthase isoform type-2	MAT2A	Homo sapiens	7.36	9.11	2	2
Q9Y240	C-type lectin domain family 11 member A	CLEC11A	Homo sapiens	6.98	8.98	2	2
P50213	Isocitrate dehyodrogenase [NAD] subunit	IDH3A	Homo sapiens	6.43	10.38	2	2
	alpha, mitochondrial
Q09028	Histone-binding protein RBBP4	RBBP4	Homo sapiens	6.37	4.94	2	2
P32969	60S ribosomal protein L9	RPL9	Homo sapiens	6.36	11.46	2	2
P21399	Cytoplasmic aconitate hydratase	ACO1	Homo sapiens	6.23	3.15	2	2
P00492	Hypoxanthine-guanine phosphoribosyltransferase	HPRT1	Homo sapiens	6.12	10.55	2	2
P49591	Serine--tRNA ligase, cytoplasmic	SARS	Homo sapiens	5.72	5.45	2	2
P30520	Adenylosuccinate synthetase isozyme 2	ADSS	Homo sapiens	5.55	4.61	2	2
P22234	Multifunctional protein ADE2 [Includes:	PAICS	Homo sapiens	5.35	6.12	2	2
	Phosphoribosylaminoimidazole-
	succinocarboxamide synthase]
P11586	C-1-tetrahydrofolate synthase, cytoplasmic	MTHFD1	Homo sapiens	5.21	2.78	2	2
Q92616	Translational activator GCN1	GCN1L1	Homo sapiens	5.02	1.05	2	2
VFE on scaffold
P02454	Collagen alpha-1(I) chain	COL1A1	Rattus norvegicus	19462.74	69.79	79	24841
P02452	Collagen alpha-1(I) chain	COL1A1	Homo sapiens	9931.72	59.97	58	13046
P02466	Collagen alpha-2(I) chain	COL1A2	Rattus norvegicus	12949.24	67.49	67	11720
P08123	Collagen alpha-2(I) chain	COL1A2	Homo sapiens	3724.87	54.32	41	3438
Q09666	Neuroblast differentiation-associated protein	AHNAK	Homo sapiens	3402.71	42.78	112	1007
	AHNAK
P12111	Collagen alpha-3(VI) chain	COL6A3	Homo sapiens	3044.09	31.82	76	895
P02461	Collagen alpha-1(III) chain	COL3A1	Homo sapiens	661.63	21.01	17	826
P02751	Fibronectin	FN1	Homo sapiens	2864.69	38.56	65	739
P12109	Collagen alpha-1(VI) chain	COL6A1	Homo sapiens	1371.07	28.02	21	680
P08670	Vimentin	VIM	Homo sapiens	2057.68	54.72	32	597
P21333	Filamin-A	FLNA	Homo sapiens	1970.03	38.23	62	483
Q15149	Plectin	PLEC	Homo sapiens	1624.20	24.68	81	445
P35579	Myosin-9	MYH9	Homo sapiens	1870.54	35.56	53	433
P60711	Actin, cytoplasmic 1	ACTB	Rattus norvegicus	1567.81	61.87	16	410
P02545	Prelamin-A/C [Cleaved into: Lamin-A/C]	LMNA	Homo sapiens	1315.37	53.16	33	387
P14618	Pyruvate kinase PKM	PKM	Homo sapiens	1486.10	61.21	28	383
P02458	Collagen alpha-1(II) chain	COL2A1	Homo sapiens	529.20	5.65	4	344
P07355	Annexin A2	AHXA2	Homo sapiens	1284.07	58.11	20	331
P11021	78 kDa glucose-regulated protein	HSPA5	Homo sapiens	1128.68	38.53	21	310
Q00610	Clathrin heavy chain 1	CLTC	Homo sapiens	1224.44	29.91	38	306
P04406	Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	Homo sapiens	1064.45	53.13	13	306
P07437	Tubulin beta chain	TUBB	Homo sapiens	1124.26	66.89	21	304
P12814	Alpha-actinin-1	ACTN1	Homo sapiens	1094.44	47.76	33	293
P68371	Tubulin beta-4B chain	TUBB4B	Homo sapiens	1083.09	70.56	22	288
P15144	Aminopeptidase N	ANPEP	Homo sapiens	990.71	27.82	25	278
P13941	Collagen alpha-1(III) chain	COL3A1	Rattus norvegicus	294.49	10.59	9	278
P11142	Heat shock cognate 71 kDa protein	HSPA8	Homo sapiens	996.23	41.33	22	263
Q13885	Tubulin beta-2A chain	TUBB2A	Homo sapiens	876.91	61.12	19	245
P12110	Collagen alpha-2(VI) chain	COL6A2	Homo sapiens	667.72	31.80	23	244
P00558	Phosphoglycerate kinase 1	PGK1	Homo sapiens	936.31	52.04	17	234
P08238	Heat shock protein HSP 90-beta	HSP90AB1	Homo sapiens	839.05	33.84	20	229
O43707	Alpha-actinin-4	ACTN4	Homo sapiens	855.61	49.62	32	228
P68363	Tubulin alpha-1B chain	TUBA1B	Homo sapiens	785.65	48.78	15	222
Q13509	Tubulin beta-3 chain	TUBB3	Homo sapiens	741.48	42.44	15	220
P08758	Annexin A5	ANXA5	Homo sapiens	758.36	67.50	18	219
P68035	Actin, alpha cardiac muscle 1	ACTC1	Rattus norvegicus	720.51	43.77	12	217
P05539	Collagen alpha-1(II) chain	COL2A1	Rattus norvegicus	361.49	5.00	3	212
P06576	ATP synthase subunit beta, mitochondrial	ATP5B	Homo sapiens	754.28	42.72	16	209
P68104	Elongation factor 1-alpha 1	EEF1A1	Homo sapiens	842.24	34.20	11	203
P07900	Heat shock protein HSP 90-alpha	HSP90AA1	Homo sapiens	727.70	29.51	19	201
P04083	Annexin A1	ANXA1	Homo sapiens	807.88	52.60	16	199
Q9Y490	Talin-1	TLN1	Homo sapiens	832.01	20.90	30	198
P14625	Endoplasmin	HSP90B1	Homo sapiens	722.94	32.88	21	197
P08133	Annexin A6	ANXA6	Homo sapiens	685.55	45.17	22	186
P50454	Serpin H1	SERPINH1	Homo sapiens	779.11	50.00	15	185
P04075	Fructose-bisphosphate aldolase A	ALDOA	Homo sapiens	742.79	65.66	17	175
P06733	Alpha-enolase	ENO1	Homo sapiens	607.87	54.15	16	168
Q04828	Aldo-keto reductase family 1 member C1	AKR1C1	Homo sapiens	671.12	49.85	12	165
P29401	Transketolase	TKT	Homo sapiens	690.60	39.33	17	157
P07237	Protein disulfide-isomerase	P4HB	Homo sapiens	602.33	48.62	21	157
P60174	Triosephosphate isomerase	TPI1	Homo sapiens	601.01	66.08	14	155
P18206	Vinculin	VCL	Homo sapiens	521.43	35.36	28	154
P06396	Gelsolin	GSN	Homo sapiens	657.62	23.66	14	152
Q16555	Dihydropyrimidinase-related protein 2	DPYSL2	Homo sapiens	619.56	48.95	17	152
P07585	Decorin	DCN	Homo sapiens	513.00	45.96	14	151
Q14764	Major vault protein	MVP	Homo sapiens	515.55	34.04	20	147
P46940	Ras GTPase-activating-like protein IQGAP1	IQGAP1	Homo sapiens	567.55	20.16	21	142
P13639	Elongation factor 2	EEF2	Homo sapiens	460.85	31.12	23	142
P30101	Protein disulfide-isomerase A3	PDIA3	Homo sapiens	482.71	42.97	16	137
Q9BUF5	Tubulin beta-6 chain	TUBB6	Homo sapiens	454.39	58.07	18	137
P07602	Proactivator polypeptide [Cleaved into:	PSAP	Homo sapiens	264.99	26.91	13	137
	Saposin-A]
P62937	Peptidyl-prolyl cis-trans isomerase A	PPIA	Homo sapiens	494.59	55.76	9	136
P62805	Histone H4	HIST1H4A	Homo sapiens	449.47	51.46	6	131
P55072	Transitional endoplasmic reticulum ATPase	VCP	Homo sapiens	519.55	43.18	23	129
P11413	Glucose-6-phosphate 1-dehydrogenase	G6PD	Homo sapiens	488.37	40.78	15	129
P18669	Phosphoglycerate mutase 1	PGAM1	Homo sapiens	467.11	44.88	9	129
O43852	Calumenin	CALU	Homo sapiens	526.31	52.38	14	127
P16152	Carbonyl reductase [NADPH] 1	CBR1	Homo sapiens	493.61	58.12	11	123
Q9NZN4	EH domain-containing protein 2	EHD2	Homo sapiens	471.67	49.91	18	122
P13489	Ribonuclease inhibitor	RNH1	Homo sapiens	471.22	44.47	13	121
P35555	Fibrillin-1	FBN1	Homo sapiens	395.19	13.79	25	114
P49327	Fatty acid synthase	FASN	Homo sapiens	444.87	15.13	23	112
P62260	14-3-3 protein epsilon	YWHAE	Rattus norvegicus	333.75	40.00	8	112
P00338	L-lactate dehydrogenase A chain	LDHA	Homo sapiens	389.25	39.16	11	111
Q99536	Synaptic vesicle membrane protein VAT-1	VAT1	Homo sapiens	418.13	35.37	8	110
	homolog
Q06830	Peroxiredoxin-1	PRDX1	Homo sapiens	432.15	56.78	10	109
P98095	Fibulin-2	FBLN2	Homo sapiens	415.65	19.85	15	109
O60814	Histone H2B type 1-K	HIST1H2BK	Homo sapiens	305.88	34.92	4	109
Q14315	Filamin-C	FLNC	Homo sapiens	459.99	11.08	17	107
Q07065	Cytoskeleton-associated protein 4	CKAP4	Homo sapiens	394.33	38.37	18	105
P27348	14-3-3 protein theta	YWHAQ	Homo sapiens	347.51	36.37	7	104
P63104	14-3-3 protein zeta/delta	YWHAZ	Homo sapiens	339.16	42.86	8	103
Q9NZM1	Myoferlin	MYOF	Homo sapiens	387.07	13.93	19	101
P10809	60 kDa heat shock protein, mitochondrial	HSPD1	Homo sapiens	386.96	31.59	14	101
P38646	Stress-70 protein, mitochondrial	HSPA9	Homo sapiens	378.75	21.50	11	100
Q14204	Cytoplasmic dynein 1 heavy chain 1	DYNC1H1	Homo sapiens	360.00	9.00	29	100
P35556	Fibrillin-2	FBN2	Homo sapiens	371.59	8.04	15	97
P67936	Tropomyosin alpha-4 chain	TPM4	Homo sapiens	342.85	47.98	13	96
P23284	Peptidyl-prolyl cis-trans isomerase B	PPIB	Homo sapiens	305.17	40.28	9	96
P26038	Moesin	MSN	Homo sapiens	353.35	26.34	15	95
P25705	ATP synthase subunit alpha, mitochondrial	ATP5A1	Homo sapiens	358.61	25.86	10	94
O00299	Chloride intracellular channel protein 1	CLIC1	Homo sapiens	355.52	68.88	12	94
P27797	Calreticulin	CALR	Homo sapiens	386.83	39.57	10	92
P42330	Aldo-keto reductase family 1 member C3	AKR1C3	Homo sapiens	386.79	42.72	9	92
P09382	Galectin-1	LGALS1	Homo sapiens	336.73	46.67	5	92
P07195	L-lactate dehydrogenase B chain	LDHB	Homo sapiens	301.77	37.72	12	92
Q6NZI2	Polymerase I and transcript release factor	PTRF	Homo sapiens	366.06	25.38	8	91
Q13813	Spectrin alpha chain, non-erythrocytic 1	SPTAN1	Homo sapiens	349.51	13.43	21	91
P23528	Cofilin-1	CFL1	Homo sapiens	381.58	57.83	10	90
P36955	Piment epithelium-derived factor	SERPINF1	Homo sapiens	334.75	22.01	7	90
P51149	Ras-related protein Rab-7a	RAB7A	Homo sapiens	325.35	60.87	10	90
P07858	Cathepsin B	CTSB	Homo sapiens	373.17	31.27	9	89
P27824	Calnexin	CANX	Homo sapiens	339.30	23.65	11	89
P40926	Malate dehydrogenase, mitochondrial	MDH2	Homo sapiens	319.47	44.38	11	89
P51884	Lumican	LUM	Homo sapiens	289.27	32.25	9	89
P53396	ATP-citrate synthase	ACLY	Homo sapiens	328.72	19.71	14	86
P40939	Trifunctional enzyme subunit alpha,	HADHA	Homo sapiens	340.14	17.96	8	83
	mitochondrial
Q96AY3	Peptidyl-prolyl cis-trans isomerase FKBP10	FKBP10	Homo sapiens	271.61	19.42	9	81
P02792	Ferritin light chain	FTL	Homo sapiens	366.87	36.00	5	80
P30041	Peroxiredoxin-6	PRDX6	Homo sapiens	281.65	48.66	9	80
P60842	Eukaryotic initiation factor 4A-I	EIF4A1	Homo sapiens	263.67	33.50	11	80
Q15084	Protein disulfide-isomerase A6	PDIA6	Homo sapiens	288.09	29.32	8	79
P04264	Keratin, type II cytoskeletal 1	KRT1	Homo sapiens	271.98	18.48	10	79
P07686	Beta-hexosaminidase subunit beta	HEXB	Homo sapiens	232.74	13.31	6	79
P49368	T-complex protein 1 subunit gamma	CCT3	Homo sapiens	283.18	29.17	11	78
P09211	Glutathione S-transferase P	GSTP1	Homo sapiens	330.61	53.33	8	77
P37802	Transgelin-2	TAGLN2	Homo sapiens	299.87	57.29	9	77
P16278	Beta-galactosidase	GLB1	Homo sapiens	281.54	24.37	11	77
P21796	Voltage-dependent anion-selective channel	VDAC1	Homo sapiens	304.95	27.21	6	76
	protein 1
P63244	Guanine nucleotide-binding protein subunit	GNB2L1	Homo sapiens	273.34	55.84	11	76
	beta-2-like 1
P11766	Alcohol dehydrogenase class-3	ADH5	Homo sapiens	338.18	22.99	6	74
P05388	60S acidic ribosomal protein P0	RPLP0	Homo sapiens	303.07	37.85	8	74
Q96AG4	Leucine-rich repeat-containing protein 59	LRRC59	Homo sapiens	280.57	31.60	6	74
Q07954	Prolow-density lipoprotein receptor-related	LRP1	Homo sapiens	276.43	4.91	15	71
	protein 1
P08865	40S ribosomal protein SA	RPSA	Homo sapiens	274.72	36.61	8	71
P61981	14-3-3 protein gamma	YWHAG	Homo sapiens	224.58	28.74	6	71
P26641	Elongation factor 1-gamma	EEF1G	Homo sapiens	271.89	28.60	11	70
O60664	Perilipin-3	PLIN3	Homo sapiens	268.33	35.02	9	70
Q01082	Spectrin beta chain, non-erythrocytic 1	SPTBN1	Homo sapiens	239.23	11.59	18	70
P09525	Annexin A4	ANXA4	Homo sapiens	228.82	34.80	9	70
O75083	WD repeat-containing protein 1	WDR1	Homo sapiens	358.76	26.73	9	69
Q96D15	Reticulocalbin-3	RCN3	Homo sapiens	299.70	30.79	6	69
Q9Y6N5	Sulfide quinone oxidoreductase,	SQRDL	Homo sapiens	253.49	28.00	10	69
	mitochondrial
P07996	Thrombospondin-1	THBS1	Homo sapiens	241.05	14.36	10	69
P40925	Malate dehydrogenase, cytoplasmic	MDH1	Homo sapiens	231.53	25.15	6	68
Q15019	Septin-2	41884	Homo sapiens	267.59	42.66	9	67
Q15365	Poly(rC)-binding protein 1	PCBP1	Homo sapiens	231.29	28.93	5	67
P07737	Profilin-1	PFN1	Homo sapiens	216.78	63.57	7	67
P62820	Ras-related protein Rab-1A	RAB1A	Homo sapiens	206.51	51.22	8	67
P06744	Glucose-6-phosphate isomerase	GPI	Homo sapiens	266.65	29.57	10	66
P22314	Ubiquitin-like modifier-activating enzyme 1	UBA1	Homo sapiens	250.03	20.42	14	66
P12882	Myosin-1	MYH1	Homo sapiens	235.28	11.55	17	65
P07339	Cathepsin D	CTSD	Homo sapiens	209.54	31.07	9	65
P18085	ADP-ribosylation factor 4	ARF4	Homo sapiens	201.61	60.56	9	65
P04844	Dolichyl-diphosphooligosaccharide--protein	RPN2	Homo sapiens	298.04	23.61	10	64
	glycosyltransferase subunit 2
Q03135	Caveolin-1	CAV1	Homo sapiens	225.24	52.25	7	64
P04792	Heat shock protein beta-1	HSPB1	Homo sapiens	217.66	39.51	7	64
P45880	Voltage-dependent anion-selective channel	VDAC2	Homo sapiens	212.61	32.99	7	64
	protein 2
P60660	Myosin light polypeptide 6	MYL6	Homo sapiens	206.89	48.34	7	64
P08107	Heat shock 70 kDa protein 1A/1B	HSPA1A	Homo sapiens	205.34	19.81	9	64
P02794	Ferritin heavy chain	FTH1	Homo sapiens	276.77	51.91	7	63
Q01518	Adenylyl cyclase-associated protein 1	CAP1	Homo sapiens	232.42	30.95	11	63
P21980	Protein-glutamine gamma-	TGM2	Homo sapiens	232.14	15.43	7	63
	glutamyltransferase 2
P05387	60S acidic ribosomal protein P2	RPLP2	Homo sapiens	256.84	69.57	4	62
Q15293	Reticulocalbin-1	RCN1	Homo sapiens	254.49	37.16	7	62
P04843	Dolichyl-diphosphooligosaccharide--protein	RPN1	Homo sapiens	235.08	30.81	12	62
	glycosyltransferase subunit 1
P55766	Puromycin-sensitive aminopeptidase	NPEPPS	Homo sapiens	200.99	13.93	10	62
Q9Y6C2	EMILIN-1	EMILIN1	Homo sapiens	210.60	14.76	11	60
P31946	14-3-3 protein beta/alpha	YWHAB	Homo sapiens	189.82	28.46	5	60
P07108	Acyl-CoA-binding protein	DBI	Homo sapiens	235.46	50.57	3	59
P50395	Rab GDP dissociation inhibitor beta	GDI2	Homo sapiens	221.44	41.80	12	59
P15121	Aldose reductase	AKR1B1	Homo sapiens	216.09	36.39	7	59
P61978	Heterogeneous nuclear ribonucleoprotein K	HNRNPK	Homo sapiens	211.91	29.59	10	59
P53618	Coatomer subunit beta	COPB1	Homo sapiens	199.75	18.68	11	59
P62241	40S ribosomal protein S8	RPS8	Homo sapiens	201.29	41.35	7	58
Q86VP6	Cullin-associated NEDD8-dissociated protein 1	CAND1	Homo sapiens	199.18	10.24	9	58
P30044	Peroxiredoxin-5, mitochondrial	PRDX5	Homo sapiens	186.74	50.00	7	58
P36578	60S ribosomal protein L4	RPL4	Homo sapiens	178.67	14.75	5	58
P61158	Actin-related protein 3	ACTR3	Homo sapiens	237.38	34.93	9	57
Q9NQC3	Reticulon-4	RTN4	Homo sapiens	227.04	9.65	6	57
P11216	Glycogen phosphorylase, brain form	PYGB	Homo sapiens	208.18	18.86	11	57
Q14108	Lysosome membrane protein 2	SCARB2	Homo sapiens	200.13	19.46	6	57
P23396	40S ribosomal protein S3	RPS3	Homo sapiens	172.16	52.26	10	57
P68871	Hemoglobin subunit beta	HBB	Homo sapiens	165.75	59.86	7	57
P12236	ADP/ATP translocase 3	SLC25A6	Homo sapiens	161.18	21.81	6	57
O60701	UDP-glucose 6-dehydrogenase	UGDH	Homo sapiens	218.40	38.06	13	56
P00387	NADH-cytochrome b5 reductase 3	CYB5R3	Homo sapiens	205.94	53.16	9	56
P30050	60S ribosomal protein L12	RPL12	Homo sapiens	202.07	54.55	6	56
P35237	Serpin B6	SERPINB6	Homo sapiens	201.97	32.18	9	56
Q96KK5	Histone H2A type 1-H	HIST1H2AH	Homo sapiens	198.46	27.34	3	56
P61204	ADP-ribosylation factor 3	ARF3	Homo sapiens	186.09	50.26	7	56
P63241	Eukaryotic translation initiation factor 5A-1	EIF5A	Homo sapiens	251.09	27.27	4	55
P49748	Very long-chain specific acyl-CoA	ACADVL	Homo sapiens	226.83	16.64	7	55
	dehydrogenase, mitochondrial
P50995	Annexin A11	ANXA11	Homo sapiens	186.26	12.87	5	55
P53621	Coatomer subunit alpha	COPA	Homo sapiens	169.57	12.01	10	55
P52209	6-phosphogluconate dehydrogenase, decarboxylating	PGD	Homo sapiens	219.84	20.50	7	54
P30086	Phosphatidylethanolamine-binding protein 1	PEBP1	Homo sapiens	214.04	64.71	7	54
Q9H299	SH3 domain-binding glutamic acid-rich-like	SH3BGRL3	Homo sapiens	210.88	31.18	3	54
	protein 3
P17655	Calpain-2 catalytic subunit	CAPN2	Homo sapiens	196.98	18.86	9	54
P22392	Nucleoside diphosphate kinase B	NME2	Homo sapiens	185.09	52.63	6	54
Q15582	Transforming growth factor-beta-induced	TGFBI	Homo sapiens	171.73	7.91	4	54
	protein ig-h3
Q14697	Neutral alpha-glucosidase AB	GANAB	Homo sapiens	244.51	13.35	9	53
P09972	Fructose-bisphosphate aldolase C	ALDOC	Homo sapiens	233.02	18.41	4	53
Q9Y686	Chloride intracellular channel protein 4	CLIC4	Homo sapiens	225.06	67.98	12	53
P13667	Protein disulfide-isomerase A4	PDIA4	Homo sapiens	216.49	17.05	8	53
P78527	DNA-dependent protein kinase catalytic	PRKDC	Homo sapiens	203.70	5.28	14	53
	subunit
P31939	Bifunctional purine biosynthesis protein	ATIC	Homo sapiens	188.54	15.71	7	52
	PURH [Includes:
	Phosphoribosylaminoimidazolecarboxamide
	formyltransferase]
P02768	Serum albumin	ALB	Homo sapiens	201.86	3.94	3	51
P52565	Rho GDP-dissociation inhibitor 1	ARHGDIA	Homo sapiens	186.59	21.08	4	51
P52907	F-actin-capping protein subunit alpha-1	CAPZA1	Homo sapiens	181.06	41.26	7	51
O75874	Isocitrate dehydrogenase [NADP]	IDH1	Homo sapiens	167.93	27.78	9	51
	cytoplasmic
P14550	Alcohol dehydrogenase [NADP(+)]	AKR1A1	Homo sapiens	176.30	23.38	7	50
P22626	Heterogeneous nuclear ribonucleoproteins A2/B1	HNRNPA2B1	Homo sapiens	166.97	28.05	8	50
P41250	Glycine-tRNA ligase	GARS	Homo sapiens	161.46	14.21	7	50
P12956	X-ray repair cross-complementing protein 6	XRCC6	Homo sapiens	156.89	23.15	10	50
Q04446	1,4-alpha-glucan-branching enzyme	GBE1	Homo sapiens	206.81	20.66	9	49
Q8IUX7	Adipocyte enhancer-binding protein 1	AEBP1	Homo sapiens	205.43	10.71	8	49
P00367	Glutamate dehydrogenase 1, mitochondrial	GLUD1	Homo sapiens	193.31	16.31	6	49
P24534	Elongation factor 1-beta	EEF1B2	Homo sapiens	183.36	28.44	5	49
P42224	Signal transducer and activator of	STAT1	Homo sapiens	176.41	20.80	12	49
	transcription 1-alpha/beta
P55209	Nucleosome assembly protein 1-like 1	NAP1L1	Homo sapiens	200.78	14.58	4	48
Q9Y678	Coatomer subunit gamma-1	COPG1	Homo sapiens	196.07	12.36	7	48
P14314	Glucosidase 2 subunit beta	PRKCSH	Homo sapiens	180.64	14.58	6	48
Q96CX2	BTB/POZ domain-containing protein KCTD12	FMOD	Homo sapiens	136.59	14.77	4	47
P50609	Fibromodulin	KCTD12	Rattus norvegicus	170.37	19.68	5	47
P50990	T-complex protein 1 subunit theta	CCT8	Homo sapiens	149.29	17.34	7	46
P10599	Thioredoxin	TXN	Homo sapiens	149.25	31.43	4	46
P30153	Serine/threonine-protein phosphatase 2A 65	PPP2R1A	Homo sapiens	182.68	18.51	7	45
	kDa regulatory subunit A alpha isoform
P55084	Trifunctional enzyme subunit beta,	HADHB	Homo sapiens	171.75	23.21	7	45
	mitochondrial
P62158	Calmodulin	CALM1	Homo sapiens	183.48	44.30	5	44
P32119	Peroxiredoxin-2	PRDX2	Homo sapiens	160.79	14.65	3	44
P49411	Elongation factor Tu, mitochondrial	TUFM	Homo sapiens	161.57	15.27	4	43
P62979	Ubiquitin-40S ribosomal protein S27a	RPS27A	Homo sapiens	152.11	33.97	4	43
O00159	Unconventional myosin-Ic	MYO1C	Homo sapiens	151.42	14.58	11	43
P34932	Heat shock 70 kDa protein 4	HSPA4	Homo sapiens	149.37	15.95	9	43
Q9P2E9	Ribosome-binding protein 1	RRBP1	Homo sapiens	147.57	11.70	10	43
Q99497	Protein DJ-1	PARK7	Homo sapiens	164.37	42.33	6	42
P62701	40S ribosomal protein S4, X isoform	RPS4X	Homo sapiens	150.60	29.66	7	42
P29692	Elongation factor 1-delta	EEF1D	Homo sapiens	136.02	32.38	7	42
Q8NBS9	Thioredoxin domain-containing protein 5	TXNDC5	Homo sapiens	165.03	18.98	6	41
P19105	Myosin regulatory light chain 12A	MYL12A	Homo sapiens	161.76	39.18	5	41
Q14974	Importin subunit beta-1	KPNB1	Homo sapiens	157.88	19.18	11	41
P39687	Acidic leucine-rich nuclear phosphoprotein 32	ANP32A	Homo sapiens	137.22	12.85	3	41
	family member A
P30048	Thioredoxin-dependent peroxide reductase, mitochondrial	PRDX3	Homo sapiens	222.53	28.91	4	40
P47755	F-actin-capping protein subunit alpha-2	CAPZA2	Homo sapiens	156.24	23.43	4	40
P17931	Galectin-3	LGALS3	Homo sapiens	154.89	25.60	5	40
P09651	Heterogeneous nuclear ribonucleoprotein A1	HNRNPA1	Homo sapiens	142.70	22.31	6	40
P51991	Heterogeneous nuclear ribonucleoprotein A3	HNRNPA3	Homo sapiens	139.93	21.96	6	40
P07384	Calpain-1 catalytic subunit	CAPN1	Homo sapiens	135.52	9.24	5	40
O95782	AP-2 complex subunit alpha-1	AP2A1	Homo sapiens	134.84	13.20	9	40
P48444	Coatomer subunit delta	ARCN1	Homo sapiens	132.91	15.46	5	40
P04080	Cystatin-B	CSTB	Homo sapiens	158.66	45.92	3	39
O43390	Heterogeneous nuclear ribonucleoprotein R	HNRNPR	Homo sapiens	142.15	8.53	4	39
P61247	40S ribosomal protein S3a	RPS3A	Homo sapiens	133.56	18.18	4	39
P04899	Guanine nucleotide-binding protein G(I)	GNAI2	Homo sapiens	131.68	17.18	4	39
	subunit alpha-2
Q16658	Fascin	FSCN1	Homo sapiens	167.69	26.37	9	38
Q07021	Complement component 1 Q subcomponent-	C1QBP	Homo sapiens	166.75	12.06	3	38
	binding protein, mitochondrial
P62829	60S ribosomal protein L23	RPL23	Homo sapiens	162.51	25.00	2	38
P07099	Epoxide hydrolase 1	EPHX1	Homo sapiens	158.47	27.47	9	38
P50991	T-complex protein 1 subunit delta	CCT4	Homo sapiens	151.63	10.76	4	38
P27105	Erythrocyte band 7 integral membrane	STOM	Homo sapiens	142.49	41.32	7	38
	protein
P15559	NAD(P)H dehydrogenase [quinone] 1	NQO1	Homo sapiens	141.57	17.15	4	38
Q00839	Heterogeneous nuclear ribonucleoprotein U	HNRNPU	Homo sapiens	135.88	15.03	7	38
P27816	Microtubule-associated protein 4	MAP4	Homo sapiens	125.87	9.64	7	38
P00966	Argininosuccinate synthase	ASS1	Homo sapiens	125.52	14.81	4	38
P19338	Nucleolin	NCL	Homo sapiens	125.15	13.66	8	38
P37837	Transaldolase	TALDO1	Homo sapiens	121.68	14.24	4	38
P06753	Tropomyosin alpha-3 chain	TPM3	Homo sapiens	119.22	13.38	5	38
Q15366	Poly(rC)-binding protein 2	PCBP2	Homo sapiens	118.11	11.78	3	38
Q01129	Decorin	DCN	Rattus norvegicus	107.47	18.64	6	38
P06748	Nucleophosmin	NPM1	Homo sapiens	144.28	24.49	6	37
P62906	60S ribosomal protein L10a	RPL10A	Homo sapiens	141.74	25.81	4	37
Q12797	Aspartyl/asparaginyl beta-hydroxylase	ASPH	Homo sapiens	139.39	15.44	8	37
P16615	Sarcoplasmic/endoplasmic reticulum calcium	ATP2A2	Homo sapiens	124.62	9.12	7	37
	ATPase 2
P46782	40S ribosomal protein S5 [Cleaved into: 40S	RPS5	Homo sapiens	122.17	17.65	3	37
	ribosomal protein S5, N-terminally processed]
Q07020	60S ribosomal protein L18	RPL18	Homo sapiens	117.66	30.85	5	37
P50914	60S ribosomal protein L14	RPL14	Homo sapiens	109.86	15.35	3	37
P62244	40S ribosomal protein S15a	RPS15A	Homo sapiens	138.62	30.00	4	36
P13645	Keratin, type I cytoskeletal 10	KRT10	Homo sapiens	117.06	21.40	9	36
P56134	ATP synthase subunit f, mitochondrial	ATP5J2	Homo sapiens	110.17	25.53	2	36
P07910	Heterogeneous nuclear ribonucleoproteins C1/C2	HNRNPC	Homo sapiens	102.07	15.03	4	36
P78371	T-complex protein 1 subunit beta	CCT2	Homo sapiens	159.70	25.61	9	35
P05534	HLA class I histocompatibility antigen, A-24	HLA-A	Homo sapiens	127.63	30.14	7	35
	alpha chain
P62888	60S ribosomal protein L30	RPL30	Homo sapiens	127.35	34.78	3	35
Q16698	2,4-dienoyl-CoA reductase, mitochondrial	DECR1	Homo sapiens	112.19	17.91	4	35
Q9Y265	RuvB-like 1	RUVBL1	Homo sapiens	110.51	23.03	7	35
P42785	Lysosomal Pro-X carboxypeptidase	PRCP	Homo sapiens	118.66	9.48	3	34
Q969G5	Protein kinase C delta-binding protein	PRKCDBP	Homo sapiens	114.93	17.24	4	34
P62424	60S ribosomal protein L7a	RPL7A	Homo sapiens	105.69	15.04	4	34
P60981	Destrin	DSTN	Homo sapiens	102.99	20.00	3	34
P62879	Guanine nucleotide-binding protein	GNB2	Homo sapiens	98.15	15.29	4	34
	G(I)/G(S)/G(T) subunit beta-2
Q92743	Serine protease HTRA1	HTRA1	Homo sapiens	97.18	11.25	5	34
P00441	Superoxide dismutase [Cu—Zn]	SOD1	Homo sapiens	191.85	32.47	4	33
Q15181	Inorganic pyrophosphatase	PPA1	Homo sapiens	145.98	35.99	7	33
P63010	AP-2 complex subunit beta	AP2B1	Homo sapiens	127.86	13.13	7	33
P15531	Nucleoside diphosphate kinase A	NME1	Homo sapiens	123.53	51.97	6	33
P08473	Neprilysin	MME	Homo sapiens	120.47	13.07	7	33
P62826	GTP-binding nuclear protein Ran	RAH	Homo sapiens	117.10	34.26	6	33
P17980	26S protease regulatory subunit 6A	PSMC3	Homo sapiens	110.28	21.41	6	33
O43776	Asparagine--tRNA ligase, cytoplasmic	NARS	Homo sapiens	107.40	19.71	8	33
P15880	40S ribosomal protein S2	RPS2	Homo sapiens	101.99	19.45	5	33
P31949	Protein S100-A11	S100A11	Homo sapiens	93.44	40.95	4	33
P68036	Ubiquitin-conjugating enzyme E2 L3	UBE2L3	Homo sapiens	143.93	35.71	3	32
P17301	Integrin alpha-2	ITGA2	Homo sapiens	128.38	6.77	5	32
P16070	CD44 antigen	CD44	Homo sapiens	126.71	3.77	2	32
P69905	Hemoglobin subunit alpha	HBA1;	Homo sapiens	115.45	28.17	3	32
P61106	Ras-related protein Rab-14	RAB14	Homo sapiens	104.55	16.74	3	32
P62249	40S ribosomal protein S16	RPS16	Homo sapiens	89.33	20.55	3	32
Q16851	UTP--glucose-1-phosphate	UGP2	Homo sapiens	150.43	12.60	4	31
	uridylyltransferase
P60033	CD81 antigen	CD81	Homo sapiens	144.01	25.00	3	31
P23142	Fibulin-1	FBLN1	Homo sapiens	139.79	20.91	7	31
P28066	Proteasome subunit alpha type-5	PSMA5	Homo sapiens	127.88	35.27	5	31
P49755	Transmembrane emp24 domain-containing	TMED10	Homo sapiens	125.42	16.89	3	31
	protein 10
Q06323	Proteasome activator complex subunit 1	PSME1	Homo sapiens	110.60	27.71	8	31
Q13838	Spliceosome RNA helicase DDX39B	DDX39B	Homo sapiens	104.76	22.43	6	31
P61586	Transforming protein RhoA	RHOA	Homo sapiens	103.88	32.64	5	31
P78539	Sushi repeat-containing protein SRPX	SRPX	Homo sapiens	94.78	7.76	3	31
P15586	N-acetylglucosamine-6-sulfatase	GNS	Homo sapiens	93.73	11.23	4	31
P59998	Actin-related protein 2/3 complex subunit 4	ARPC4	Homo sapiens	92.41	35.71	5	31
Q9BSJ8	Extended synaptotagmin-1	ESYT1	Homo sapiens	92.29	7.34	6	31
P25396	40S ribosomal protein S12	RPS12	Homo sapiens	129.03	31.82	3	30
Q5T9L3	Protein wntless homolog	WLS	Homo sapiens	127.49	9.80	4	30
P20810	Calpastatin	CAST	Homo sapiens	124.88	10.88	4	30
P00325	Alcohol dehydrogenase 1B	ADH1B	Homo sapiens	118.70	26.67	7	30
P23219	Prostaglandin G/H synthase 1	PTGS1	Homo sapiens	113.71	12.85	5	30
Q00325	Phosphate carrier protein, mitochondrial	SLC25A3	Homo sapiens	102.13	19.06	5	30
P46926	Glucosamine-6-phosphate isomerase 1	GNPDA1	Homo sapiens	93.67	21.11	4	30
P28838	Cytosol aminopeptidase	LAP3	Homo sapiens	120.58	14.64	5	29
P17856	6-phosphofructokinase, liver type	PFKL	Homo sapiens	112.91	14.49	8	29
Q96TA1	Niban-like protein 1	FAM129B	Homo sapiens	108.73	14.88	7	29
P62277	40S ribosomal protein S13	RPS13	Homo sapiens	106.57	18.54	3	29
Q96QK1	Vacuolar protein sorting-associated protein	VPS35	Homo sapiens	100.71	12.06	7	29
	35
P39019	40S ribosomal protein S19	RPS19	Homo sapiens	94.38	30.34	6	29
Q13162	Peroxiredoxin-4	PRDX4	Homo sapiens	93.42	37.64	7	29
Q14103	Heterogeneous nuclear ribonucleoprotein D0	HNRNPD	Homo sapiens	86.53	14.65	4	29
Q6NXT2	Histone H3.3C	H3F3C	Homo sapiens	77.14	11.85	2	29
P26599	Polypyrimidine tract-binding protein 1	PTBP1	Homo sapiens	129.28	12.62	4	28
P62942	Peptidyl-prolyl cis-trans isomerase FKBP1A	FKBP1A	Homo sapiens	129.28	29.63	3	28
P18124	60S ribosomal protein L7	RPL7	Homo sapiens	105.73	23.39	4	28
P98160	Basement membrane-specific heparan	HSPG2	Homo sapiens	104.99	2.37	7	28
	sulfate proteoglycan core protein
P62140	Serine/threonine-protein phosphatase PP1-	PPP1CB	Homo sapiens	102.12	18.96	5	28
	beta catalytic subunit
P51688	N-sulphoglucosamine sulphohydrolase	SGSH	Homo sapiens	101.57	8.37	3	28
P21399	Cytoplasmic aconitate hydratase	ACO1	Homo sapiens	98.99	11.81	7	28
P08253	72 kDa type IV collagenase	MMP2	Homo sapiens	95.34	10.91	4	28
O00571	ATP-dependent RNA helicase DDX3X	DDX3X	Homo sapiens	91.46	9.67	5	28
Q9Y2Q3	Glutathione S-transferase kappa 1	GSTK1	Homo sapiens	90.89	33.63	6	28
P35232	Prohibitin	PHB	Homo sapiens	88.99	28.31	6	28
Q9Y277	Voltage-dependent anion-selective channel	VDAC3	Homo sapiens	81.75	14.49	3	28
	protein 3
P51659	Peroxisomal multifunctional enzyme type 2	HSD17B4	Homo sapiens	106.47	10.87	5	27
Q05682	Caldesmon	CALD1	Homo sapiens	88.52	5.17	4	27
P46783	40S ribosomal protein S10	RPS10	Homo sapiens	86.11	14.55	2	27
P83731	60S ribosomal protein L24	RPL24	Homo sapiens	80.95	20.38	3	27
P30040	Endoplasmic reticulum resident protein 29	ERP29	Homo sapiens	73.20	14.18	3	27
P62269	40S ribosomal protein S18	RPS18	Homo sapiens	71.59	16.45	3	27
Q96HE7	ERO1-like protein alpha	ERO1L	Homo sapiens	114.32	22.44	6	26
Q16181	Septin-7	41889	Homo sapiens	100.36	21.97	6	26
P43235	Cathepsin K	CTSK	Homo sapiens	99.90	19.45	3	26
P40261	Nicotinamide N-methyltransferase	NNMT	Homo sapiens	99.01	21.97	4	26
O43399	Tumor protein D54	TPD52L2	Homo sapiens	96.02	36.41	5	26
P21266	Glutathione S-transferase Mu 3	ALB	Homo sapiens	84.67	22.67	4	26
P08962	CD63 antigen	GSTM3	Homo sapiens	72.76	7.56	2	26
P02770	Serum albumin	CD63	Rattus norvegicus	93.78	4.28	2	26
P22695	Cytochrome b-c1 complex subunit 2,	UQCRC2	Homo sapiens	104.25	16.78	5	25
	mitochondrial
P39023	60S ribosomal protein L3	RPL3	Homo sapiens	103.60	20.84	6	25
Q00341	Vigilin	HDLBP	Homo sapiens	103.20	4.10	3	25
Q01813	6-phosphofructokinase type C	PFKP	Homo sapiens	97.67	13.14	6	25
P12955	Xaa-Pro dipeptidase	PEPD	Homo sapiens	92.15	8.32	3	25
P27487	Dipeptidyl peptidase 4	DPP4	Homo sapiens	90.72	10.44	6	25
P05413	Fatty acid-binding protein, heart	FABP3	Homo sapiens	88.34	45.86	6	25
P35908	Keratin, type II cytoskeletal 2 epidermal	KRT2	Homo sapiens	85.90	11.58	6	25
P21589	5′-nucleotidase	NT5E	Homo sapiens	85.75	15.85	6	25
O75915	PRA1 family protein 3	ARL6IP5	Homo sapiens	81.97	15.96	2	25
P05556	Integrin beta-1	ITGB1	Homo sapiens	80.76	6.02	4	25
Q8WUM4	Programmed cell death 6-interacting protein	PDCD6IP	Homo sapiens	74.74	7.37	5	25
P78417	Glutathione S-transferase omega-1	GSTO1	Homo sapiens	74.27	7.88	2	25
P46781	40S ribosomal protein S9	RPS9	Homo sapiens	68.19	13.40	3	25
P14866	Heterogeneous nuclear ribonucleoprotein L	HNRNPL	Homo sapiens	123.31	8.66	2	24
P40227	T-complex protein 1 subunit zeta	CCT6A	Homo sapiens	112.14	25.05	7	24
Q12905	Interleukin enhancer-binding factor 2	ILF2	Homo sapiens	105.67	12.82	3	24
Q7KZF4	Staphylococcal nuclease domain-containing	SND1	Homo sapiens	99.88	13.19	7	24
	protein 1
P02765	Alpha-2-HS-glycoprotein	AHSG	Homo sapiens	90.97	5.45	2	24
O60506	Heterogeneous nuclear ribonucleoprotein Q	SYNCRIP	Homo sapiens	90.57	11.88	5	24
P23634	Plasma membrane calcium-transporting	ATP2B4	Homo sapiens	85.12	2.50	2	24
	ATPase 4
P10155	60 kDa SS-A/Ro ribonucleoprotein	TROVE2	Homo sapiens	79.15	4.46	2	24
O00231	26S proteasome non-ATPase regulatory	PSMD11	Homo sapiens	75.89	8.77	3	24
	subunit 11
P61088	Ubiquitin-conjugating enzyme E2 N	UBE2N	Homo sapiens	71.94	41.45	5	24
P05386	60S acidic ribosomal protein P1	RPLP1	Homo sapiens	110.40	51.75	2	23
O15460	Prolyl 4-hydroxylase subunit alpha-2	P4HA2	Homo sapiens	101.78	12.90	5	23
P13693	Translationally-controlled tumor protein	TPT1	Homo sapiens	94.17	12.21	2	23
P10620	Microsomal glutathione S-transferase 1	MGST1	Homo sapiens	89.77	27.10	3	23
Q99623	Prohibitin-2	PHB2	Homo sapiens	82.71	25.42	6	23
P61604	10 kDa heat shock protein, mitochondrial	HSPE1	Homo sapiens	82.34	25.49	2	23
Q8NHP8	Putative phospholipase B-like 2	PLBD2	Homo sapiens	81.14	8.49	4	23
P35754	Glutaredoxin-1	GLRX	Homo sapiens	75.29	30.19	2	23
P17987	T-complex protein 1 subunit alpha	TCP1	Homo sapiens	66.48	8.45	4	23
P16403	Histone H1.2	HIST1H1C	Homo sapiens	63.40	19.72	4	23
P01033	Metalloproteinase inhibitor 1	TIMP1	Homo sapiens	90.30	28.02	4	22
P60866	40S ribosomal protein S20	RPS20	Homo sapiens	87.96	19.33	2	22
P04179	Superoxide dismutase [Mn], mitochondrial	SOD2	Homo sapiens	84.22	22.97	4	22
P01889	HLA class I histocompatibility antigen, B-7	HLA-B	Homo sapiens	80.41	20.72	5	22
	alpha chain
Q9UBG0	C-type mannose receptor 2	MRC2	Homo sapiens	79.01	4.12	4	22
O94979	Protein transport protein Sec31A	SEC31A	Homo sapiens	75.45	4.67	4	22
P07711	Cathepsin L1	CTSL	Homo sapiens	72.15	8.41	2	22
P25789	Proteasome subunit alpha type-4	PSMA4	Homo sapiens	67.06	21.46	3	22
P61160	Actin-related protein 2	ACTR2	Homo sapiens	63.07	12.94	4	22
Q9UHD8	Septin-9	41891	Homo sapiens	62.81	13.14	5	22
P35268	60S ribosomal protein L22	RPL22	Homo sapiens	62.65	18.75	2	22
P30084	Enoyl-CoA hydratase, mitochondrial	ECHS1	Homo sapiens	96.93	19.31	3	21
O14773	Tripeptidyl-peptidase 1	TPP1	Homo sapiens	81.36	8.17	3	21
P62333	26S protease regulatory subunit 10B	PSMC6	Homo sapiens	79.86	11.83	3	21
P54709	Sodium/potassium-transporting ATPase	ATP1B3	Homo sapiens	76.45	17.20	3	21
	subunit beta-3
P13010	X-ray repair cross-complementing protein 5	XRCC5	Homo sapiens	76.09	11.34	5	21
P39656	Dolichyl-diphosphooligosaccharide--protein	DDOST	Homo sapiens	75.49	16.23	5	21
	glycosyltransferase 48 kDa subunit
Q16881	Thioredoxin reductase 1, cytoplasmic	TXNRD1	Homo sapiens	73.59	10.02	4	21
Q9NR45	Sialic acid synthase	NANS	Homo sapiens	73.04	15.88	3	21
P13674	Prolyl 4-hydroxylase subunit alpha-1	P4HA1	Homo sapiens	72.31	14.98	6	21
P47756	F-actin-capping protein subunit beta	CAPZB	Homo sapiens	71.92	20.94	4	21
P61313	60S ribosomal protein L15	RPL15	Homo sapiens	70.62	17.16	3	21
P11047	Laminin subunit gamma-1	LAMC1	Homo sapiens	66.17	5.90	7	21
P51148	Ras-related protein Rab-5C	RAB5C	Homo sapiens	63.90	23.15	4	21
O75390	Citrate synthase, mitochondrial	CS	Homo sapiens	60.12	7.73	3	21
P62263	40S ribosomal protein S14	RPS14	Homo sapiens	87.52	21.19	2	20
Q13724	Mannosyl-oligosaccharide glucosidase	MOGS	Homo sapiens	84.85	3.58	2	20
P36543	V-type proton ATPase subunit E 1	ATP6V1E1	Homo sapiens	79.06	6.19	2	20
P13797	Plastin-3	PLS3	Homo sapiens	70.30	10.79	5	20
P54920	Alpha-soluble NSF attachment protein	NAPA	Homo sapiens	68.68	28.47	6	20
P27635	60S ribosomal protein L10	RPL10	Homo sapiens	64.27	23.83	3	20
Q04760	Lactoylglutathione lyase	GLO1	Homo sapiens	63.58	14.67	2	20
P26640	Valine--tRNA ligase	VARS	Homo sapiens	62.47	3.88	4	20
O94905	Erlin-2	ERLIN2	Homo sapiens	61.85	16.81	4	20
O14979	Heterogeneous nuclear ribonucleoprotein D-	HNRNPDL	Homo sapiens	61.35	9.76	3	20
	like
P13473	Lysosome-associated membrane	LAMP2	Homo sapiens	57.75	7.07	3	20
	glycoprotein 2
P11940	Polyadenylate-binding protein 1	PABPC1	Homo sapiens	56.47	9.91	5	20
P20340	Ras-related protein Rab-6A	RAB6A	Homo sapiens	54.52	22.12	4	20
P62318	Small nuclear ribonucleoprotein Sm D3	SNRPD3	Homo sapiens	53.58	15.08	2	20
Q99460	26S proteasome non-ATPase regulatory	PSMD1	Homo sapiens	109.07	5.46	3	19
	subunit 1
Q9BS26	Endoplasmic reticulum resident protein 44	ERP44	Homo sapiens	90.29	15.27	4	19
Q15185	Prostaglandin E synthase 3	PTGES3	Homo sapiens	87.82	18.13	2	19
P29373	Cellular retinoic acid-binding protein 2	CRABP2	Homo sapiens	84.96	34.78	4	19
P20618	Proteasome subunit beta type-1	PSMB1	Homo sapiens	79.58	21.16	3	18
Q9Y3I0	tRNA-splicing ligase RtcB homolog	RTCB	Homo sapiens	75.39	13.47	4	19
O14818	Proteasome subunit alpha type-7	PSMA7	Homo sapiens	74.40	28.63	4	19
P51636	Caveolin-2	CAV2	Homo sapiens	71.42	20.99	2	19
P35606	Coatomer subunit beta′	COPB2	Homo sapiens	67.84	10.49	6	19
Q15836	Vesicle-associated membrane protein 3	VAMP3	Homo sapiens	67.54	33.00	2	19
P21810	Biglycan	BGN	Homo sapiens	65.73	10.87	3	19
Q99715	Collagen alpha-1(XII) chain	COL12A1	Homo sapiens	63.88	3.40	7	19
Q13557	Calcium/calmodulin-dependent protein kinase	CAMK2D	Homo sapiens	63.08	16.03	5	19
	type II subunit delta
P00505	Aspartate aminotransferase, mitochondrial	GOT2	Homo sapiens	60.55	14.42	5	19
P02786	Transferrin receptor protein 1	TFRC	Homo sapiens	56.99	5.00	3	19
P04062	Glucosylceramidase	GBA	Homo sapiens	54.01	4.48	2	19
Q9NVA2	Septin-11	41893	Homo sapiens	51.50	8.86	3	19
Q8IWE2	Protein NOXP20	FAM114A1	Homo sapiens	76.12	14.03	5	18
Q9NVJ2	ADP-ribosylation factor-like protein 8B	ARL8B	Homo sapiens	72.50	34.95	5	18
P07814	Bifunctional glutamate/proline--tRNA ligase	EPRS	Homo sapiens	71.04	4.70	4	18
Q9UNM6	26S proteasome non-ATPase regulatory	PSMD13	Homo sapiens	69.95	18.62	5	18
	subunit 13
Q13200	26S proteasome non-ATPase regulatory	PSMD2	Homo sapiens	68.87	9.47	6	18
	subunit 2
Q07960	Rho GTPase-activating protein 1	ARHGAP1	Homo sapiens	68.44	14.35	4	18
P36957	Dihydrolipoyllysine-residue	DLST	Homo sapiens	66.31	12.80	4	18
	succinyltransferase component of 2-
	oxoglutarate dehydrogenase complex, mitochondrial
Q13492	Phosphatidylinositol-binding clathrin assembly protein	PICALM	Homo sapiens	61.69	4.91	2	18
P13804	Electron transfer flavoprotein subunit alpha,	ETFA	Homo sapiens	59.21	25.53	6	18
	mitochondrial
P62753	40S ribosomal protein S6	RPS6	Homo sapiens	57.11	18.88	4	18
P11279	Lysosome-associated membrane glycoprotein 1	LAMP1	Homo sapiens	54.87	10.55	4	18
Q15075	Early endosome antigen 1	EEA1	Homo sapiens	54.84	4.61	5	18
Q9UHA4	Ragulator complex protein LAMTOR3	LAMTOR3	Homo sapiens	53.15	30.65	2	18
P49773	Histidine triad nucleotide-binding protein 1	HINT1	Homo sapiens	114.80	53.97	3	17
P04632	Calpain small subunit 1	CAPNS1	Homo sapiens	72.67	44.40	5	17
Q9H4M9	EH domain-containing protein 1	EHD1	Homo sapiens	72.21	14.42	5	17
O75367	Core histone macro-H2A 1	H2AFY	Homo sapiens	72.16	13.44	3	17
O15144	Actin-related protein 2/3 complex subunit 2	ARPC2	Homo sapiens	66.66	21.67	4	17
Q9NYU2	UDP-glucose: glycoprotein	UGGT1	Homo sapiens	63.95	6.17	6	17
	glucosyltransferase 1
Q9UIJ7	GTP: AMP phosphotransferase AK3, mitochondrial	AK3	Homo sapiens	62.60	18.50	3	17
Q7L2H7	Eukaryotic translation initiation factor 3	EIF3M	Homo sapiens	60.36	15.78	4	17
	subunit M
P62857	40S ribosomal protein S28	RPS28	Homo sapiens	58.87	33.33	2	17
Q02878	60S ribosomal protein L6	RPL6	Homo sapiens	58.47	15.63	3	17
Q15436	Protein transport protein Sec23A	SEC23A	Homo sapiens	57.10	3.53	2	17
P61224	Ras-related protein Rap-1b	RAP1B	Homo sapiens	57.06	27.72	4	17
P51571	Translocon-associated protein subunit delta	SSR4	Homo sapiens	55.54	23.12	3	17
P26447	Protein S100-A4	S100A4	Homo sapiens	53.79	28.71	3	17
Q14647	LIM and SH3 domain protein 1	LASP1	Homo sapiens	53.29	21.84	5	17
Q12884	Seprase	FAP	Homo sapiens	52.54	7.63	5	17
P02795	Metallothionein-2	MT2A	Homo sapiens	51.33	21.31	2	17
P38159	RNA-binding motif protein, X chromosome	RBMX	Homo sapiens	48.65	8.95	3	17
P62081	40S ribosomal protein S7	RPS7	Homo sapiens	77.61	38.66	4	16
P21964	Catechol O-methyltransferase	COMT	Homo sapiens	69.40	19.93	4	16
P48047	ATP synthase subunit O, mitochondrial	ATP5O	Homo sapiens	66.42	39.44	5	16
P62195	26S protease regulatory subunit 8	PSMC5	Homo sapiens	55.47	18.23	5	16
P20674	Cytochrome c oxidase subunit 5A,	COX5A	Homo sapiens	51.48	26.67	3	16
	mitochondrial
Q9UHG3	Prenylcysteine oxidase 1	PCYOX1	Homo sapiens	48.05	11.88	4	16
P10301	Ras-related protein R-Ras	RRAS	Homo sapiens	47.42	12.84	2	16
P20700	Lamin-B1	LMNB1	Homo sapiens	43.93	5.46	4	16
P37235	Hippocalcin-like protein 1	HPCAL1	Homo sapiens	40.95	17.10	3	16
P39060	Collagen alpha-1(XVIII) chain [Cleaved into:	COL18A1	Homo sapiens	85.90	4.45	4	15
	Endostatin]
P63173	60S ribosomal protein L38	RPL38	Homo sapiens	73.59	35.71	2	15
O43242	26S proteasome non-ATPase regulatory	PSMD3	Homo sapiens	65.74	7.68	3	15
	subunit 3
P46777	60S ribosomal protein L5	RPL5	Homo sapiens	65.57	23.91	5	15
P63167	Dynein light chain 1, cytoplasmic	DYNLL1	Homo sapiens	64.99	37.08	2	15
Q9BWD1	Acetyl-CoA acetyltransferase, cytosolic	ACAT2	Homo sapiens	63.92	22.92	4	15
P19623	Spermidine synthase	SRM	Homo sapiens	63.90	37.09	6	15
P12268	Inosine-5′-monophosphate dehydrogenase 2	IMPDH2	Homo sapiens	63.68	5.84	2	15
P61769	Beta-2-microglobulin [Cleaved into: Beta-2-	B2M	Homo sapiens	62.44	35.29	3	15
	microglobulin form pl 5.3]
P09622	Dihydrolipoyl dehydrogenase, mitochondrial	DLD	Homo sapiens	62.33	6.48	2	15
O95373	Importin-7	IPO7	Homo sapiens	61.73	4.43	3	15
P49257	Protein ERGIC-53	LMAN1	Homo sapiens	60.05	10.39	2	15
P00390	Glutathione reductase, mitochondrial	GSR	Homo sapiens	52.89	11.88	3	15
P61019	Ras-related protein Rab-2A	RAB2A	Homo sapiens	50.25	23.58	4	15
P23141	Liver carboxylesterase 1	CES1	Homo sapiens	50.07	7.58	3	15
O60763	General vesicular transport factor p115	USO1	Homo sapiens	49.72	6.24	4	15
Q9H8H3	Methyltransferase-like protein 7A	METTL7A	Homo sapiens	48.17	12.30	2	15
P09960	Leukotriene A-4 hydrolase	LTA4H	Homo sapiens	47.92	6.71	3	15
Q9UHL4	Dipeptidyl peptidase 2	DPP7	Homo sapiens	44.72	4.47	2	15
Q01995	Transgelin	TAGLN	Homo sapiens	44.12	21.39	3	15
P41252	Isoleucine--tRNA ligase, cytoplasmic	IARS	Homo sapiens	39.65	3.49	4	15
P61970	Nuclear transport factor 2	NUTF2	Homo sapiens	66.79	33.86	2	14
Q92499	ATP-dependent RNA helicase DDX1	DDX1	Homo sapiens	66.78	7.43	3	14
O14980	Exportin-1	XPO1	Homo sapiens	65.25	5.23	3	14
P68402	Platelet-activating factor acetylhydrolase IB	PAFAH1B2	Homo sapiens	53.86	20.96	3	14
	subunit beta
P31943	Heterogeneous nuclear ribonucleoprotein H	HNRNPH1	Homo sapiens	52.62	14.25	4	14
P46778	60S ribosomal protein L21	RPL21	Homo sapiens	47.80	27.50	3	14
Q32P28	Prolyl 3-hydroxylase 1	LEPRE1	Homo sapiens	47.36	5.71	3	14
O95816	BAG family molecular chaperone regulator 2	BAG2	Homo sapiens	46.92	13.27	2	14
Q92945	Far upstream element-binding protein 2	KHSRP	Homo sapiens	45.76	5.63	3	14
P62847	40S ribosomal protein S24	RPS24	Homo sapiens	45.06	20.30	2	14
Q9ULV4	Coronin-1C	CORO1C	Homo sapiens	43.61	9.07	3	14
Q99584	Protein S100-A13	S100A13	Homo sapiens	43.40	23.47	2	14
Q9Y3A6	Transmembrane emp24 domain-containing	TMED5	Homo sapiens	42.79	10.04	2	14
	protein 5
P62750	60S ribosomal protein L23a	RPL23A	Homo sapiens	42.75	21.15	3	14
O15260	Surfeit locus protein 4	SURF4	Homo sapiens	42.65	11.90	3	14
P19404	NADH dehydrogenase [ubiquinone]	NDUFV2	Homo sapiens	42.13	9.24	2	14
	flavoprotein 2, mitochondrial
P48681	Nestin	NES	Homo sapiens	39.33	1.48	2	14
Q9NQW7	Xaa-Pro aminopeptidase 1	XPNPEP1	Homo sapiens	38.25	4.82	2	14
Q99829	Copine-1	CPNE1	Homo sapiens	37.60	4.66	2	14
P06703	Protein S100-A6	S100A6	Homo sapiens	36.86	36.67	2	14
P61163	Alpha-centractin	ACTR1A	Homo sapiens	55.95	14.89	3	13
Q08211	ATP-dependent RNA helicase A	DHX9	Homo sapiens	54.67	6.14	5	13
P38606	V-type proton ATPase catalytic subunit A	ATP6V1A	Homo sapiens	54.37	17.34	6	13
Q99714	3-hydroxyacyl-CoA dehydrogenase type-2	HSD17B10	Homo sapiens	54.15	31.03	4	13
P27701	CD82 antigen	CD82	Homo sapiens	49.98	10.49	2	13
Q13765	Nascent polypeptide-associated complex	NACA	Homo sapiens	49.28	18.60	3	13
	subunit alpha
P14854	Cytochrome c oxidase subunit 6B1	COX6B1	Homo sapiens	48.91	47.67	3	13
P06756	Integrin alpha-V	ITGAV	Homo sapiens	45.52	11.45	7	13
P26373	60S ribosomal protein L13	RPL13	Homo sapiens	44.56	15.64	3	13
Q02809	Procollagen-lysine, 2-oxoglutarate 5-	PLOD1	Homo sapiens	44.26	7.43	4	13
	dioxygenase 1
P62899	60S ribosomal protein L31	RPL31	Homo sapiens	42.96	18.40	2	13
P60953	Cell division control protein 42 homolog	CDC42	Homo sapiens	42.70	20.42	3	13
Q6NUM9	All-trans-retinol 13,14-reductase	RETSAT	Homo sapiens	41.53	4.92	2	13
Q01105	Protein SET	SET	Homo sapiens	40.71	18.26	4	13
P05455	Lupus La protein	SSB	Homo sapiens	39.96	12.50	4	13
P31948	Stress-induced-phosphoprotein 1	STIP1	Homo sapiens	39.18	7.73	3	13
P54136	Arginine--tRNA ligase, cytoplasmic	RARS	Homo sapiens	38.86	10.61	6	13
Q92841	Probable ATP-dependent RNA helicase	DDX17	Homo sapiens	38.01	7.27	5	13
	DDX17
P62330	ADP-ribosylation factor 6	ARF6	Homo sapiens	37.62	12.57	2	13
Q13228	Selenium-binding protein 1	SELENBP1	Homo sapiens	37.11	13.98	5	13
Q14152	Eukaryotic translation initiation factor 3	EIF3A	Homo sapiens	37.07	4.27	5	13
	subunit A
P05023	Sodium/potassium-transporting ATPase	ATP1A1	Homo sapiens	36.70	5.28	4	13
	subunit alpha-1
Q70UQ0	Inhibitor of nuclear factor kappa-B kinase-	IKBIP	Homo sapiens	36.29	6.57	2	13
	interacting protein
O14579	Coatomer subunit epsilon	COPE	Homo sapiens	57.73	6.82	2	12
P46063	ATP-dependent DNA helicase Q1	RECQL	Homo sapiens	51.17	6.63	3	12
Q9UBQ7	Glyoxylate reductase/hydroxypyruvate	GRHPR	Homo sapiens	49.69	17.38	3	12
	reductase
Q07666	KH domain-containing, RNA-binding, signal	KHDRBS1	Homo sapiens	47.70	7.67	2	12
	transduction-associated protein 1
Q16363	Laminin subunit alpha-4	LAMA4	Homo sapiens	46.26	4.55	6	12
P46821	Microtubule-associated protein 1B	MAP1B	Homo sapiens	46.16	4.62	7	12
P24752	Acetyl-CoA acetyltransferase, mitochondrial	ACAT1	Homo sapiens	42.51	9.84	3	12
O75368	SH3 domain-binding glutamic acid-rich-like protein	SH3BGRL	Homo sapiens	42.33	35.09	3	12
O95292	Vesicle-associated membrane protein-	VAPB	Homo sapiens	40.84	10.70	2	12
	associated protein B/C
Q13418	Integrin-linked protein kinase	ILK	Homo sapiens	40.30	14.38	4	12
P80303	Nucleobindin-2	NUCB2	Homo sapiens	40.20	7.14	2	12
P49589	Cysteine-tRNA ligase, cytoplasmic	CARS	Homo sapiens	40.10	4.68	2	12
Q9P2J5	Leucine-tRNA ligase, cytoplasmic	LARS	Homo sapiens	39.68	2.98	3	12
P23526	Adenosylhomocysteinase	AHCY	Homo sapiens	39.58	13.43	4	12
Q14112	Nidogen-2	NID2	Homo sapiens	39.09	7.71	6	12
P55884	Eukaryotic translation initiation factor 3	EIF3B	Homo sapiens	38.13	5.90	4	12
	subunit B
O15145	Actin-related protein 2/3 complex subunit 3	ARPC3	Homo sapiens	37.32	13.48	2	12
P02649	Apolipoprotein E	APOE	Homo sapiens	37.22	8.52	2	12
P55795	Heterogeneous nuclear ribonucleoprotein H2	HNRNPH2	Homo sapiens	36.67	10.47	3	12
Q99439	Calponin-2	CNN2	Homo sapiens	36.58	13.27	3	12
P62917	60S ribosomal protein L8	RPL8	Homo sapiens	34.54	25.29	5	12
P60900	Proteasome subunit alpha type-6	PSMA6	Homo sapiens	34.38	10.16	2	12
Q71UM5	40S ribosomal protein S27-like	RPS27L	Homo sapiens	33.77	38.10	3	12
Q03252	Lamin-B2	LMNB2	Homo sapiens	33.19	9.50	6	12
Q15323	Keratin, type I cuticular Ha1	KRT31	Homo sapiens	56.86	10.34	3	11
Q15121	Astrocytic phosphoprotein PEA-15	PEA15	Homo sapiens	54.65	27.69	3	11
Q6DD88	Atlastin-3	ATL3	Homo sapiens	53.61	11.46	3	11
O15511	Actin-related protein 2/3 complex subunit 5	ARPC5	Homo sapiens	53.45	20.53	2	11
Q9Y3F4	Serine-threonine kinase receptor-associated protein	STRAP	Homo sapiens	49.03	8.86	2	11
P49591	Serine--tRNA ligase, cytoplasmic	SARS	Homo sapiens	48.84	16.54	5	11
P07954	Fumarate hydratase, mitochondrial	FH	Homo sapiens	48.14	8.63	2	11
Q15008	26S proteasome non-ATPase regulatory	PSMD6	Homo sapiens	46.03	7.20	2	11
	subunit 6
Q6IAA8	Ragulator complex protein LAMTOR1	LAMTOR1	Homo sapiens	45.57	15.53	2	11
P23246	Splicing factor, proline- and glutamine-rich	SFPQ	Homo sapiens	44.03	6.93	4	11
Q02618	Nucleobindin-1	NUCB1	Homo sapiens	41.49	6.29	2	11
Q9Y5X1	Sorting nexin-9	SNX9	Homo sapiens	41.27	7.39	3	11
P31937	3-hydroxyisobutyrate dehydrogenase,	HIBADH	Homo sapiens	40.12	7.14	2	11
	mitochondrial
Q99832	T-complex protein 1 subunit eta	CCT7	Homo sapiens	39.69	11.97	4	11
P22087	rRNA 2′-O-methyltransferase fibrillarin	FBL	Homo sapiens	38.41	12.15	3	11
Q9NR31	GTP-binding protein SAR1a	SAR1A	Homo sapiens	37.09	21.72	3	11
P43487	Ran-specific GTPase-activating protein	RANBP1	Homo sapiens	36.66	16.92	2	11
Q8IZP2	Putative protein FAM10A4	ST13P4	Homo sapiens	36.63	17.08	3	11
P51858	Hepatoma-derived growth factor	HDGF	Homo sapiens	36.37	13.75	2	11
Q13263	Transcription intermediary factor 1-beta	TRIM28	Homo sapiens	35.01	5.87	3	11
O60282	Kinesin heavy chain isoform 5C	KIF5C	Homo sapiens	34.89	2.51	2	11
Q9NZ08	Endoplasmic reticulum aminopeptidase 1	ERAP1	Homo sapiens	33.59	5.84	4	11
Q13561	Dynactin subunit 2	DCTN2	Homo sapiens	33.10	8.23	2	11
Q14258	E3 ubiquitin/ISG15 ligase TRIM25	TRIM25	Homo sapiens	32.68	6.03	3	11
P00403	Cytochrome c oxidase subunit 2	MT-CO2	Homo sapiens	31.78	20.26	3	11
Q15417	Calponin-3	CNN3	Homo sapiens	29.35	7.60	2	11
P14406	Cytochrome c oxidase subunit 7A2,	COX7A2	Homo sapiens	28.49	27.71	2	11
	mitochondrial
P28799	Granulins	GRN	Homo sapiens	48.40	8.43	2	10
P28331	NADH-ubiquinone oxidoreductase 75 kDa	NDUFS1	Homo sapiens	48.36	7.84	3	10
	subunit, mitochondrial
Q92597	Protein NDRG1	NDRG1	Homo sapiens	44.52	8.88	2	10
P48643	T-complex protein 1 subunit epsilon	CCT5	Homo sapiens	44.52	12.20	4	10
P00568	Adenylate kinase isoenzyme 1	AK1	Homo sapiens	44.50	25.77	4	10
O00410	Importin-5	IPO5	Homo sapiens	44.31	5.10	3	10
P04216	Thy-1 membrane glycoprotein	THY1	Homo sapiens	42.19	15.53	2	10
P62191	26S protease regulatory subunit 4	PSMC1	Homo sapiens	41.71	8.18	2	10
P61916	Epididymal secretory protein E1	NPC2	Homo sapiens	41.08	25.83	2	10
Q53GQ0	Estradiol 17-beta-dehydrogenase 12	HSD17B12	Homo sapiens	40.53	14.10	3	10
P09429	High mobility group protein B1	HMGB1	Homo sapiens	38.34	21.86	3	10
Q9NTK5	Obg-like ATPase 1	OLA1	Homo sapiens	38.07	11.11	3	10
Q96CW1	AP-2 complex subunit mu	AP2M1	Homo sapiens	36.90	15.17	4	10
P99999	Cytochrome c	CYCS	Homo sapiens	36.48	24.76	2	10
P22102	Trifunctional purine biosynthetic protein	GART	Homo sapiens	35.51	2.67	2	10
	adenosine-3 [Includes: Phosphoribosylamine--
	glycine ligase]
P38117	Electron transfer flavoprotein subunit beta	ETFB	Homo sapiens	35.36	10.20	2	10
P10253	Lysosomal alpha-glucosidase	GAA	Homo sapiens	34.48	4.94	3	10
P14868	Aspartate--tRNA ligase, cytoplasmic	DARS	Homo sapiens	34.20	7.78	3	10
P18621	60S ribosomal protein L17	RPL17	Homo sapiens	32.59	17.93	3	10
P46977	Dolichyl-diphosphooligosaccharide--protein	STT3A	Homo sapiens	32.58	3.69	2	10
	glycosyltransferase subunit STT3A
P55735	Protein SEC13 homolog	SEC13	Homo sapiens	30.57	15.22	3	10
Q9Y646	Carboxypeptidase Q	CPQ	Homo sapiens	29.57	4.24	2	10
O95747	Serine/threonine-protein kinase OSR1	OXSR1	Homo sapiens	28.78	4.93	2	10
Q16795	NADH dehydrogenase [ubiquinone] 1 alpha	NDUFA9	Homo sapiens	28.28	9.28	3	10
	subcomplex subunit 9, mitochondrial
Q13425	Beta-2-syntrophin	SNTB2	Homo sapiens	27.91	3.89	2	10
Q9HC38	Glyoxalase domain-containing protein 4	GLOD4	Homo sapiens	26.47	7.35	2	10
P22307	Non-specific lipid-transfer protein	SCP2	Homo sapiens	25.98	3.47	2	10
P25787	Proteasome subunit alpha type-2	PSMA2	Homo sapiens	41.91	27.78	4	9
Q02952	A-kinase anchor protein 12	AKAP12	Homo sapiens	41.76	7.58	6	9
P09619	Platelet-derived growth factor receptor beta	PDGFRB	Homo sapiens	41.28	5.24	3	9
O00154	Cytosolic acyl coenzyme A thioester hydrolase	ACOT7	Homo sapiens	36.65	12.37	3	9
O14880	Microsomal glutathione S-transferase 3	MGST3	Homo sapiens	36.64	23.03	2	9
O15143	Actin-related protein 2/3 complex subunit 1B	ARPC1B	Homo sapiens	36.57	18.55	4	9
O75131	Copine-3	CPNE3	Homo sapiens	34.80	6.33	2	9
P80723	Brain acid soluble protein 1	BASP1	Homo sapiens	33.15	18.94	3	9
Q1KMD3	Heterogeneous nuclear ribonucleoprotein U-	HNRNPUL2	Homo sapiens	32.68	4.82	2	9
	like protein 2
Q9Y4L1	Hypoxia up-regulated protein 1	HYOU1	Homo sapiens	31.81	6.81	4	9
Q9Y371	Endophilin-B1	SH3GLB1	Homo sapiens	28.93	7.40	2	9
O43615	Mitochondrial import inner membrane	TIMM44	Homo sapiens	28.91	5.09	2	9
	translocase subunit TIM44
Q9Y3U8	60S ribosomal protein L36	RPL36	Homo sapiens	26.89	21.90	3	9
P35613	Basigin	BSG	Homo sapiens	26.77	8.31	2	9
O94832	Unconventional myosin-Id	MYO1D	Homo sapiens	26.14	2.49	2	9
Q9BWM7	Sideroflexin-3	SFXN3	Homo sapiens	26.01	8.62	2	9
P84098	60S ribosomal protein L19	RPL19	Homo sapiens	25.73	13.27	2	9
Q9UM54	Unconventional myosin-VI	MYO6	Homo sapiens	25.66	2.16	2	9
P62851	40S ribosomal protein S25	RPS25	Homo sapiens	24.91	16.00	2	9
P27695	DNA-(apurinic or apyrimidinic site) lyase	APEX1	Homo sapiens	41.98	29.87	5	8
P30085	UMP-CMP kinase	CMPK1	Homo sapiens	37.21	16.84	2	8
P10768	S-formylglutathione hydrolase	ESD	Homo sapiens	34.83	25.18	4	8
Q9Y224	UPF0568 protein C14orf166	C14ORF166	Homo sapiens	30.19	22.54	4	8
P43490	Nicotinamide phosphoribosyltransferase	NAMPT	Homo sapiens	29.74	8.35	2	8
P43243	Matrin-3	MATR3	Homo sapiens	28.76	7.20	4	8
P54578	Ubiquitin carboxyl-terminal hydrolase 14	USP14	Homo sapiens	27.29	5.87	2	8
Q9UJ70	N-acetyl-D-glucosamine kinase	NAGK	Homo sapiens	24.55	17.73	4	8
P52272	Heterogeneous nuclear ribonucleoprotein M	HNRNPM	Homo sapiens	24.43	4.79	3	8
O95336	6-phosphogluconolactonase	PGLS	Homo sapiens	24.35	17.05	3	8
P0CW22	40S ribosomal protein S17-like	RPS17L	Homo sapiens	24.28	39.26	4	8
P27708	CAD protein [Includes: Glutamine-dependent	CAD	Homo sapiens	22.05	0.90	2	8
	carbamoyl-phosphate synthase]
Q02543	60S ribosomal protein L18a	RPL18A	Homo sapiens	19.31	8.52	2	8
P48147	Prolyl endopeptidase	PREP	Homo sapiens	32.74	7.61	3	7
Q9ULZ3	Apoptosis-associated speck-like protein	PYCARD	Homo sapiens	31.58	20.00	3	7
	containing a CARD
P09936	Ubiquitin carboxyl-terminal hydrolase	UCHL1	Homo sapiens	30.85	30.04	4	7
	isozyme L1
P28161	Glutathione S-transferase Mu 2	GSTM2	Homo sapiens	28.86	28.90	4	7
O75955	Flotillin-1	FLOT1	Homo sapiens	27.63	11.24	3	7
P53007	Tricarboxylate transport protein,	SLC25A1	Homo sapiens	27.37	11.25	3	7
	mitochondrial
Q02218	2-oxoglutarate dehydrogenase, mitochondrial	OGDH	Homo sapiens	26.92	7.72	4	7
P23381	Tryptophan--tRNA ligase, cytoplasmic	WARS	Homo sapiens	25.94	6.16	2	7
P19367	Hexokinase-1	HK1	Homo sapiens	25.59	4.14	3	7
Q969H8	UPF0556 protein C19orf10	C19ORF10	Homo sapiens	24.70	15.03	2	7
P07942	Laminin subunit beta-1	LAMB1	Homo sapiens	23.93	2.69	3	7
P06865	Beta-hexosaminidase subunit alpha	HEXA	Homo sapiens	23.56	7.75	3	7
Q63ZY3	KN motif and ankyrin repeat domain-	KANK2	Homo sapiens	22.46	4.70	3	7
	containing protein 2
Q9UQ80	Proliferation-associated protein 2G4	PA2G4	Homo sapiens	22.38	13.20	3	7
Q96FQ6	Protein S100-A16	S100A16	Homo sapiens	22.13	22.33	2	7
P67775	Serine/threonine-protein phosphatase 2A	PPP2CA	Homo sapiens	21.41	15.86	3	7
	catalytic subunit alpha isoform
P05091	Aldehyde dehydrogenase, mitochondrial	ALDH2	Homo sapiens	21.31	5.80	2	7
Q16718	NADH dehydrogenase [ubiquinone] 1 alpha	NDUFA5	Homo sapiens	20.43	22.41	2	7
	subcomplex subunit 5
P10644	cAMP-dependent protein kinase type I-alpha	PRKAR1A	Homo sapiens	20.35	9.19	2	7
	regulatory subunit
P55010	Eukaryotic translation initiation factor 5	EIF5	Homo sapiens	20.27	5.57	2	7
P20042	Eukaryotic translation initiation factor 2	EIF2S2	Homo sapiens	20.09	8.11	2	7
	subunit 2
P61353	60S ribosomal protein L27	RPL27	Homo sapiens	19.64	21.32	2	7
Q13451	Peptidyl-prolyl cis-trans isomerase FKBP5	FKBP5	Homo sapiens	19.41	6.56	2	7
O60488	Long-chain-fatty-acid--CoA ligase 4	ACSL4	Homo sapiens	18.27	4.36	2	7
P62913	60S ribosomal protein L11	RPL11	Homo sapiens	17.77	12.92	2	7
P40429	60S ribosomal protein L13a	RPL13A	Homo sapiens	17.62	8.87	2	7
P43304	Glycerol-3-phosphate dehydrogenase,	GPD2	Homo sapiens	31.91	6.05	2	6
	mitochondrial
P51665	26S proteasome non-ATPase regulatory	PSMD7	Homo sapiens	31.27	12.65	2	6
	subunit 7
Q16643	Drebrin	DBN1	Homo sapiens	29.77	6.32	2	6
P31930	Cytochrome b-c1 complex subunit 1,	UQCRC1	Homo sapiens	29.16	8.54	2	6
	mitochondrial
O43491	Band 4.1-like protein 2	EPB41L2	Homo sapiens	28.75	3.58	2	6
P21281	V-type proton ATPase subunit B, brain	ATP6V1B2	Homo sapiens	28.46	6.65	2	6
	isoform
O00151	PDZ and LIM domain protein 1	PDLIM1	Homo sapiens	27.03	17.33	3	6
Q08380	Galectin-3-binding protein	LGALS3BP	Homo sapiens	22.65	7.52	3	6
Q15063	Periostin	POSTN	Homo sapiens	22.25	4.31	2	6
P30043	Flavin reductase	BLVRB	Homo sapiens	21.34	12.14	2	6
P39059	Collagen alpha-1(XV) chain [Cleaved into:	COL15A1	Homo sapiens	19.86	2.88	2	6
	Restin]
Q13126	S-methyl-5′-thioadenosine phosphorylase	MTAP	Homo sapiens	19.56	20.49	3	6
P36871	Phosphoglucomutase-1	PGM1	Homo sapiens	19.51	7.83	3	6
P49821	NADH dehydrogenase [ubiquinone]	NDUFV1	Homo sapiens	19.40	7.97	2	6
	flavoprotein 1, mitochondrial
P13798	Acylamino-acid-releasing enzyme	APEH	Homo sapiens	19.12	4.78	2	6
P35998	26S protease regulatory subunit 7	PSMC2	Homo sapiens	19.00	5.08	2	6
P48735	Isocitrate dehydrogenase [NADP],	IDH2	Homo sapiens	18.47	5.97	2	6
	mitochondrial
P42765	3-ketoacyl-CoA thiolase, mitochondrial	ACAA2	Homo sapiens	18.27	7.81	2	6
Q9Y262	Eukaryotic translation initiation factor 3	EIF3L	Homo sapiens	18.24	3.55	2	6
	subunit L
P10515	Dihydrolipoyllysine-residue acetyltransferase	DLAT	Homo sapiens	17.45	5.87	3	6
	component of pyruvate dehydrogenase
	complex, mitochondrial
P52630	Signal transducer and activator of	STAT2	Homo sapiens	17.26	2.82	2	6
	transcription 2
Q9Y376	Calcium-binding protein 39	CAB39	Homo sapiens	16.98	6.16	2	6
Q9Y230	RuvB-like 2	RUVBL2	Homo sapiens	16.90	8.21	3	6
Q15758	Neutral amino acid transporter B(0)	SLC1A5	Homo sapiens	16.35	4.44	2	6
Q13488	V-type proton ATPase 116 kDa subunit a isoform 3	TCIRG1	Homo sapiens	16.17	2.65	2	6
P67812	Signal peptidase complex catalytic subunit	SEC11A	Homo sapiens	15.79	9.50	2	6
	SEC11A
Q9BTV4	Transmembrane protein 43	TMEM43	Homo sapiens	15.70	9.00	2	6
P54819	Adenylate kinase 2, mitochondrial	AK2	Homo sapiens	26.93	13.81	2	5
Q08431	Lactadherin	MFGE8	Homo sapiens	23.70	8.53	2	5
Q15363	Transmembrane emp24 domain-containing	TMED2	Homo sapiens	22.92	13.93	2	5
	protein 2
Q99798	Aconitate hydratase, mitochondrial	ACO2	Homo sapiens	21.59	7.18	3	5
P47897	Glutamine--tRNA ligase	QARS	Homo sapiens	20.09	3.74	2	5
Q9BVK6	Transmembrane emp24 domain-containing	TMED9	Homo sapiens	19.41	14.47	2	5
	protein 9
P49720	Proteasome subunit beta type-3	PSMB3	Homo sapiens	18.74	23.90	3	5
O75947	ATP synthase subunit d, mitochondrial	ATP5H	Homo sapiens	18.55	23.60	2	5
Q8NC51	Plasminogen activator inhibitor 1 RNA-	SERBP1	Homo sapiens	17.47	12.01	3	5
	binding protein
Q14247	Src substrate cortactin	CTTN	Homo sapiens	17.46	4.73	2	5
P56537	Eukaryotic translation initiation factor 6	EIF6	Homo sapiens	16.72	13.88	2	5
P32969	60S ribosomal protein L9	RPL9	Homo sapiens	16.46	15.63	3	5
P53004	Biliverdin reductase A	BLVRA	Homo sapiens	16.33	10.47	2	5
Q8IVL8	Prolyl 3-hydroxylase 3	LEPREL2	Homo sapiens	16.32	4.21	2	5
Q99961	Endophilin-A2	SH3GL1	Homo sapiens	16.27	12.23	3	5
P34897	Serine hydroxymethyltransferase, mitochondrial	SHMT2	Homo sapiens	16.25	4.96	2	5
O75489	NADH dehydrogenase [ubiquinone] iron-	NDUFS3	Homo sapiens	15.69	9.09	2	5
	sulfur protein 3, mitochondrial
P54802	Alpha-N-acetylglucosaminidase	NAGLU	Homo sapiens	15.09	3.77	2	5
P22105	Tenascin-X	TNXB	Homo sapiens	14.73	0.44	2	5
P78344	Eukaryotic translation initiation factor 4	EIF4G2	Homo sapiens	14.65	2.32	2	5
	gamma 2
Q15392	Delta(24)-sterol reductase	DHCR24	Homo sapiens	14.52	5.23	2	5
P11586	C-1-tetrahydrofolate synthase, cytoplasmic	MTHFD1	Homo sapiens	14.51	7.49	4	5
P00491	Purine nucleoside phosphorylase	PNP	Homo sapiens	14.37	9.69	2	5
Q16891	Mitochondrial inner membrane protein	IMMT	Homo sapiens	13.52	4.35	2	5
Q7L576	Cytoplasmic FMR1-interacting protein 1	CYFIP1	Homo sapiens	13.35	1.84	2	5
O60749	Sorting nexin-2	SNX2	Homo sapiens	13.17	5.97	3	5
Q13510	Acid ceramidase	ASAH1	Homo sapiens	23.59	10.13	2	4
Q15942	Zyxin	ZYX	Homo sapiens	22.85	6.64	2	4
P05198	Eukaryotic translation initiation factor 2	EIF2S1	Homo sapiens	19.39	13.65	2	4
	subunit 1
P26639	Threonine--tRNA ligase, cytoplasmic	TARS	Homo sapiens	18.59	7.61	3	4
Q9UL46	Proteasome activator complex subunit 2	PSME2	Homo sapiens	17.60	12.13	2	4
Q9Y2B0	Protein canopy homolog 2	CNPY2	Homo sapiens	16.38	17.03	2	4
Q04837	Single-stranded DNA-binding protein,	SSBP1	Homo sapiens	16.36	22.30	2	4
	mitochondrial
P04040	Catalase	CAT	Homo sapiens	16.25	6.07	2	4
Q9BQG0	Myb-binding protein 1A	MYBBP1A	Homo sapiens	14.03	3.92	4	4
P24539	ATP synthase subunit b, mitochondrial	ATP5F1	Homo sapiens	13.65	14.84	3	4
Q92896	Golgi apparatus protein 1	GLG1	Homo sapiens	13.63	4.92	3	4
O00764	Pyridoxal kinase	PDXK	Homo sapiens	12.53	9.29	2	4
P08195	4F2 cell-surface antigen heavy chain	SLC3A2	Homo sapiens	12.44	4.13	2	4
P11177	Pyruvate dehydrogenase E1 component	PDHB	Homo sapiens	12.43	8.91	2	4
	subunit beta, mitochondrial
P42704	Leucine-rich PPR motif-containing protein,	LRPPRC	Homo sapiens	12.09	2.44	3	4
	mitochondrial
Q9NSE4	Isoleucine--tRNA ligase, mitochondrial	IARS2	Homo sapiens	12.01	3.16	3	4
Q09028	Histone-binding protein RBBP4	RBBP4	Homo sapiens	11.31	4.94	2	4
Q13641	Trophoblast glycoprotein	TPBG	Homo sapiens	11.29	6.90	2	4
Q8TED1	Probable glutathione peroxidase 8	GPX8	Homo sapiens	11.12	14.35	2	4
P02533	Keratin, type I cytoskeletal 14	KRT14	Homo sapiens	10.56	5.72	3	4
P63220	40S ribosomal protein S21	RPS21	Homo sapiens	9.98	22.89	2	4
P29966	Myristoylated alanine-rich C-kinase substrate	MARCKS	Homo sapiens	15.74	19.28	2	3
P13861	cAMP-dependent protein kinase type II-alpha	PRKAR2A	Homo sapiens	14.72	7.92	2	3
	regulatory subunit
Q16401	26S proteasame non-ATPase regulatory	PSMD5	Homo sapiens	13.99	6.35	2	3
	subunit 5
P15848	Arylsulfatase B	ARSB	Homo sapiens	11.33	4.69	2	3
P60983	Glia maturation factor beta	GMFB	Homo sapiens	10.95	23.94	2	3
P51648	Fatty aldehyde dehydrogenase	ALDH3A2	Homo sapiens	10.57	6.39	2	3
Q92905	COP9 signalosome complex subunit 5	COPS5	Homo sapiens	9.88	6.89	2	3
O00754	Lysosomal alpha-mannosidase	MAN2B1	Homo sapiens	9.77	4.06	3	3
P00492	Hypoxanthine-guanine phosphoribosyltransferase	HPRT1	Homo sapiens	9.69	10.55	2	3
Q8NBJ5	Procollagen galatcosyltransferase 1	COLGALT1	Homo sapiens	9.09	3.70	2	3
P22234	Multifunctional protein ADE2 [Includes:	PAICS	Homo sapiens	8.98	6.59	2	3
	Phosphoribosylaminoimidazole-
	succinocarboxamide synthase]
Q92973	Transportin-1	TNPO1	Homo sapiens	8.69	4.01	2	3
O00203	AP-3 complex subunit beta-1	AP3B1	Homo sapiens	8.64	2.19	2	3
Q13619	Cullin-4A	CUL4A	Homo sapiens	8.46	3.16	2	3
O14737	Programmed cell death protein 5	PDCD5	Homo sapiens	8.38	17.60	2	3
P61009	Signal peptidase complex subunit 3	SPCS3	Homo sapiens	7.69	12.78	2	3
P24821	Tenascin	TNC	Homo sapiens	10.38	2.18	2	2
O43504	Ragulator complex protein LAMTOR5	LAMTOR5	Homo sapiens	9.61	43.96	2	2
P58546	Myotrophin	MTPN	Homo sapiens	8.58	23.73	2	2
O43809	Cleavage and polyadenylation specificity	NUDT21	Homo sapiens	7.44	14.54	2	2
	factor subunit 5
Q9Y240	C-type lectin domain family 11 member A	CLEC11A	Homo sapiens	7.24	8.98	2	2
P15289	Arylsulfatase A	ARSA	Homo sapiens	6.60	6.11	2	2
Q6P2Q9	Pre-mRNA-processing-splicing factor 8	PRPF8	Homo sapiens	5.80	1.24	2	2
Engineered VF mucosa
P02454	Collagen alpha-1(I) chain	COL1A1	Rattus norvegicus	17939.13	67.17	76	23308
P02452	Collagen alpha-1(I) chain	COL1A1	Homo sapiens	9692.90	59.90	58	12942
P02466	Collagen alpha-2(I) chain	COL1A2	Rattus norvegicus	12133.22	66.84	65	11242
P08123	Collagen alpha-2(I) chain	COL1A2	Homo sapiens	3559.41	53.00	41	3375
Q09666	Neuroblast differentiation-associated protein	AHNAK	Homo sapiens	4681.98	48.56	130	1337
	AHNAK
P02461	Collagen alpha-1(III) chain	COL3A1	Homo sapiens	1086.61	25.24	23	1275
P12111	Collagen alpha-3(VI) chain	COL6A3	Homo sapiens	3130.59	32.61	82	922
P12109	Collagen alpha-1(VI) chain	COL6A1	Homo sapiens	1617.48	30.74	22	761
P02751	Fibronectin	FN1	Homo sapiens	2824.49	38.14	62	711
P08670	Vimentin	VIM	Homo sapiens	2203.33	54.72	32	629
P21333	Filamin-A	FLNA	Homo sapiens	2379.16	38.12	65	583
Q15149	Plectin	PLEC	Homo sapiens	1740.04	24.12	79	495
P35579	Myosin-9	MYH9	Homo sapiens	2004.50	38.72	58	485
P02545	Prelamin-A/C [Cleaved into: Lamin-A/C]	LMNA	Homo sapiens	1569.62	56.78	36	449
P60711	Actin, cytoplasmic 1	ACTB	Rattus norvegicus	1773.20	63.20	16	446
P14618	Pyruvate kinase PKM	PKM	Homo sapiens	1609.00	59.70	26	406
P07437	Tubulin beta chain	TUBB	Homo sapiens	1561.07	68.92	22	406
P07355	Annexin A2	ANXA2	Homo sapiens	1571.61	61.95	21	399
P68371	Tubulin beta-4B chain	TUBB4B	Homo sapiens	1482.06	72.58	23	382
P04406	Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	Homo sapiens	1281.24	48.36	14	338
P15144	Aminopeptidase N	ANPEP	Homo sapiens	1205.90	25.44	24	325
Q13885	Tubulin beta-2A chain	TUBB2A	Homo sapiens	1196.37	65.17	21	319
Q00610	Clathrin heavy chain 1	CLTC	Homo sapiens	1321.28	26.81	34	315
P11021	78 kDa glucose-regulated protein	HSPA5	Homo sapiens	1056.41	37.31	20	293
P02458	Collagen alpha-1(II) chain	COL2A1	Homo sapiens	434.49	5.65	4	291
Q13509	Tubulin beta-3 chain	TUBB3	Homo sapiens	964.94	40.67	15	286
P68104	Elongation factor 1-alpha 1	EEF1A1	Homo sapiens	1185.32	34.20	11	285
O43707	Alpha-actinin-4	ACTN4	Homo sapiens	1087.17	46.65	32	284
P12814	Alpha-actinin-1	ACTN1	Homo sapiens	1005.57	37.33	26	274
P13941	Collagen alpha-1(III) chain	COL3A1	Rattus norvegicus	328.06	11.69	10	273
P68363	Tubulin alpha-1B chain	TUBA1B	Homo sapiens	936.85	47.01	16	269
P12110	Collagen alpha-2(VI) chain	COL6A2	Homo sapiens	749.27	28.46	21	268
P00558	Phosphoglycerate kinase 1	PGK1	Homo sapiens	1079.13	52.04	17	265
P11142	Heat shock cognate 71 kDa protein	HSPA8	Homo sapiens	954.88	39.94	21	246
P08238	Heat shock protein HSP 90-beta	HSP90AB1	Homo sapiens	895.47	33.01	18	234
P06576	ATP synthase subunit beta, mitochondrial	ATP5B	Homo sapiens	847.43	42.72	15	232
Q9Y490	Talin-1	TLN1	Homo sapiens	941.93	18.30	27	229
P68035	Actin, alpha cardiac muscle 1	ACTC1	Rattus norvegicus	734.75	40.85	11	226
P08758	Annexin A5	ANXA5	Homo sapiens	788.33	67.19	18	213
P50454	Serpin H1	SERPINH1	Homo sapiens	914.27	43.78	14	210
P06733	Alpha-enolase	ENO1	Homo sapiens	747.38	48.85	16	209
P04083	Annexin A1	ANXA1	Homo sapiens	841.45	50.00	15	206
P07900	Heat shock protein HSP 90-alpha	HSP90AA1	Homo sapiens	758.22	22.81	15	204
P14625	Endoplasmin	HSP90B1	Homo sapiens	738.44	30.88	20	202
P08133	Annexin A6	ANXA6	Homo sapiens	735.45	43.54	22	195
P60174	Triosephosphate isomerase	TPI1	Homo sapiens	760.58	66.08	14	190
Q14764	Major vault protein	MVP	Homo sapiens	622.39	32.03	20	180
Q04828	Aldo-keto reductase family 1 member C1	AKR1C1	Homo sapiens	706.40	43.96	12	178
Q9BUF5	Tubulin beta-6 chain	TUBB6	Homo sapiens	606.14	38.79	14	178
P04075	Fructose-bisphosphate aldolase A	ALDOA	Homo sapiens	754.78	60.99	15	176
P46940	Ras GTPase-activating-like protein IQGAP1	IQGAP1	Homo sapiens	687.20	19.79	23	175
P04264	Keratin, type II cytoskeletal 1	KRT1	Homo sapiens	612.98	36.34	24	173
P13639	Elongation factor 2	EEF2	Homo sapiens	541.61	29.95	23	169
P06396	Gelsolin	GSN	Homo sapiens	729.67	24.68	14	165
P35555	Fibrillin-1	FBN1	Homo sapiens	596.23	17.24	30	164
Q16555	Dihydropyrimidinase-related protein 2	DPYSL2	Homo sapiens	640.36	41.96	15	159
Q9NZN4	EH domain-containing protein 2	EHD2	Homo sapiens	632.37	38.31	16	159
Q14315	Filamin-C	FLNC	Homo sapiens	690.18	10.94	17	158
O60814	Histone H2B type 1-K	HIST1H2BK	Homo sapiens	439.34	28.57	3	157
P62937	Peptidyl-prolyl cis-trans isomerase A	PPIA	Homo sapiens	553.28	55.76	9	156
P29401	Transketolase	TKT	Homo sapiens	648.74	33.71	15	155
P18669	Phosphoglycerate mutase 1	PGAM1	Homo sapiens	546.57	45.28	10	149
P00338	L-lactate dehydrogenase A chain	LDHA	Homo sapiens	503.58	37.95	11	147
O43852	Calumenin	CALU	Homo sapiens	598.11	42.54	13	146
P07237	Protein disulfide-isomerase	P4HB	Homo sapiens	552.43	38.58	17	144
Q13813	Spectrin alpha chain, non-erythrocytic 1	SPTAN1	Homo sapiens	514.56	13.55	22	134
P11413	Glucose-6-phosphate 1-dehydrogenase	G6PD	Homo sapiens	503.50	36.89	13	134
P18206	Vinculin	VCL	Homo sapiens	461.06	28.22	22	134
P30101	Protein disulfide-isomerase A3	PDIA3	Homo sapiens	465.94	37.23	15	132
P07585	Decorin	DCN	Homo sapiens	444.03	43.18	12	130
P55072	Transitional endoplasmic reticulum ATPase	VCP	Homo sapiens	506.92	32.13	18	129
P11216	Glycogen phosphorylase, brian form	PYGB	Homo sapiens	485.45	28.47	19	127
Q07065	Cytoskeleton-associated protein 4	CKAP4	Homo sapiens	472.22	41.86	20	127
P27348	14-3-3 protein theta	YWHAQ	Homo sapiens	444.22	42.45	9	127
P62260	14-3-3 protein epsilon	YWHAE	Rattus norvegicus	375.64	40.00	8	124
P63104	14-3-3 protein zeta/delta	YWHAZ	Homo sapiens	432.53	41.22	8	120
Q06830	Peroxiredoxin-1	PRDX1	Homo sapiens	480.66	60.30	10	119
P09382	Galectin-1	LGALS1	Homo sapiens	434.09	46.67	5	119
P16152	Carbonyl reductase [NADPH] 1	CBR1	Homo sapiens	478.30	52.35	10	118
P67936	Tropomyosin alpha-4 chain	TPM4	Homo sapiens	419.41	48.79	14	118
O00299	Chloride intracellular channel protein 1	CLIC1	Homo sapiens	454.50	60.17	10	116
P62805	Histone H4	HIST1H4A	Homo sapiens	407.94	52.43	7	116
Q14204	Cytoplasmic dynein 1 heavy chain 1	DYNC1H1	Homo sapiens	395.38	7.68	26	113
P07195	L-lactate dehydrogenase B chain	LDHB	Homo sapiens	366.01	37.72	12	113
P27797	Calreticulin	CALR	Homo sapiens	468.52	37.41	9	111
P13489	Ribonuclease inhibitor	RNH1	Homo sapiens	451.04	40.35	12	111
Q99536	Synaptic vesicle membrane protein VAT-1	VAT1	Homo sapiens	425.08	32.06	8	106
	homolog
P63244	Guanine nucleotide-binding protein subunit	GNB2L1	Homo sapiens	387.23	58.68	12	104
	beta-2-like 1
Q01082	Spectrin beta chain, non-erythrocytic 1	SPTBN1	Homo sapiens	384.44	12.10	20	104
P22314	Ubiquitin-like modifier-activating enzyme 1	UBA1	Homo sapiens	382.34	23.72	16	104
P10809	60 kDa heat shock protein, mitochondrial	HSPD1	Homo sapiens	394.59	27.05	12	103
P23264	Peptidyl-prolyl cis-trans isomerase B	PPIB	Homo sapiens	334.48	36.57	8	103
Q03135	Caveolin-1	CAV1	Homo sapiens	373.84	63.48	9	102
P09211	Glutathione S-transferase P	GSTP1	Homo sapiens	429.56	53.33	8	101
O60664	Perilipin-3	PLIN3	Homo sapiens	386.15	37.79	10	101
P53396	ATP-citrate synthase	ACLY	Homo sapiens	383.16	17.80	13	101
P08107	Heat shock 70 kDa protein 1A/1B	HSPA1A	Homo sapiens	357.20	28.71	14	101
P42330	Aldo-keto reductase family 1 member C3	AKR1C3	Homo sapiens	412.36	35.60	9	99
P25705	ATP synthase subunit alpha, mitochondrial	ATP5A1	Homo sapiens	390.18	22.24	9	98
P23528	Cofilin-1	CFL1	Homo sapiens	402.40	57.23	9	97
Q6NZI2	Polymerase I and transcript release factor	PTRF	Homo sapiens	370.34	25.64	9	96
Q9NZM1	Myoferlin	MYOF	Homo sapiens	359.24	14.65	20	96
P07602	Proactivator polypeptide [Cleaved into:	PSAP	Homo sapiens	229.01	20.23	10	96
	Saposin-A]
P40926	Malate dehydrogenase, mitochondrial	MDH2	Homo sapiens	368.82	42.31	10	95
P38955	Pigment epithelium-derived factor	SERPINF1	Homo sapiens	342.17	25.84	10	95
P35556	Fibrillin-2	FBN2	Homo sapiens	354.09	7.14	14	92
P38646	Stress-70 protein, mitochondrial	HSPA9	Homo sapiens	350.09	21.06	10	91
P40939	Trifunctional enzyme subunit alpha,	HADHA	Homo sapiens	366.81	17.17	8	90
	mitochondrial
P51884	Lumican	LUM	Homo sapiens	301.46	29.29	8	90
P21980	Protein-glutamine gamma-glutamyltransferase 2	TGM2	Homo sapiens	335.23	20.96	10	89
P49368	T-complex protein 1 subunit gamma	CCT3	Homo sapiens	331.43	29.17	11	89
P05388	60S acidic ribosomal protein P0	RPLP0	Homo sapiens	366.21	37.85	8	88
P37802	Transgelin-2	TAGLN2	Homo sapiens	348.12	46.73	8	88
P51149	Ras-related protein Rab-7a	RAB7A	Homo sapiens	315.29	42.51	7	88
Q15019	Septin-2	41884	Homo sapiens	343.22	42.66	9	86
Q96AY3	Peptidyl-prolyl cis-trans isomerase FKBP10	FKBP10	Homo sapiens	287.18	20.79	9	85
P26038	Moesin	MSN	Homo sapiens	334.03	23.22	13	84
P27824	Calnexin	CANX	Homo sapiens	327.37	19.26	9	84
P06744	Glucose-6-phosphate isomerase	GPI	Homo sapiens	340.63	27.78	9	83
P61978	Hetergeneous nuclear ribonucleoprotein K	HNRNPK	Homo sapiens	314.43	29.59	10	83
P11766	Alcohol dehydrogenase class-3	ADH5	Homo sapiens	385.13	22.99	6	82
P49327	Fatty acid synthase	FASN	Homo sapiens	344.44	10.51	16	82
P21796	Voltage-dependent anion-selective channel	VDAC1	Homo sapiens	326.64	27.21	6	82
	protein 1
P30041	Peroxiredoxin-6	PRDX6	Homo sapiens	299.10	44.64	8	82
Q86VP6	Cullin-associated NEDD8-dissociated protein 1	CAND1	Homo sapiens	279.60	11.30	10	82
P60660	Myosin light polypeptide 6	MYL6	Homo sapiens	263.81	48.34	7	82
P09525	Annexin A4	ANXA4	Homo sapiens	276.12	35.11	9	81
P53618	Coatomer subunit beta	COPB1	Homo sapiens	240.03	17.00	11	77
P98095	Fibulin-2	FBLN2	Homo sapiens	297.15	17.15	13	76
P07858	Cathepsin B	CTSB	Homo sapiens	325.77	28.02	7	75
P05387	60S acidic ribosomal protein P2	RPLP2	Homo sapiens	312.25	76.52	5	75
P31946	14-3-3 protein beta/alpha	YWHAB	Homo sapiens	256.23	32.52	6	74
P61981	14-3-3 protein gamma	YWHAG	Homo sapiens	253.62	20.65	4	74
P07686	Beta-hexosaminidase subunit beta	HEXB	Homo sapiens	223.90	11.69	5	74
O75083	WD repeat-containing protein 1	WDR1	Homo sapiens	403.80	26.24	8	73
Q04446	1,4-alpha-glucan-branching enzyme	GBE1	Homo sapiens	322.94	20.66	9	73
P45880	Voltage-dependent anion-selective channel	VDAC2	Homo sapiens	262.24	25.85	5	73
	protein 2
P04844	Dolichyl-diphosphooligosaccharide--protein	RPN2	Homo sapiens	295.08	28.68	11	72
	glycosyltransferase subunit 2
P08865	40S ribosomal protein SA	RPSA	Homo sapiens	285.15	36.95	8	72
P00387	NADH-cytochrome b5 reductase 3	CYB5R3	Homo sapiens	280.81	31.56	7	71
P78527	DNA-dependent protein kinase catalytic	PRKDC	Homo sapiens	267.13	5.77	15	71
	subunit
P02792	Ferritin light chain	FTL	Homo sapiens	323.33	36.00	5	70
P52209	6-phosphogluconate dehydrogenase,	PGD	Homo sapiens	281.42	18.22	6	70
	decarboxylating
P02768	Serum albumin	ALB	Homo sapiens	281.23	3.94	3	70
P09972	Fructose-bisphosphate aldolase C	ALDOC	Homo sapiens	303.97	22.53	5	69
Q07954	Prolow-density lipoprotein receptor-related	LRP1	Homo sapiens	255.24	5.02	16	69
	protein 1
P07339	Cathepsin D	CTSD	Homo sapiens	246.82	20.63	6	69
Q15582	Transforming growth factor-beta-induced	TGFBI	Homo sapiens	219.77	10.54	6	69
	protein ig-h3
P07737	Profilin-1	PFN1	Homo sapiens	216.88	64.29	8	69
Q9Y678	Coatomer subunit gamma-1	COPG1	Homo sapiens	274.16	10.41	6	68
P26641	Elongation factor 1-gamma	EEF1G	Homo sapiens	269.96	24.49	8	68
Q96AG4	Leucine-rich repeat-containing protein 59	LRRC59	Homo sapiens	259.13	31.60	6	68
P61158	Actin-related protein 3	ACTR3	Homo sapiens	296.73	27.75	8	67
Q15084	Protein disulfide-isomerase A6	PDIA6	Homo sapiens	252.32	32.50	9	67
P50995	Annexin A11	ANXA11	Homo sapiens	233.06	12.87	5	67
P62241	40S ribosomal protein S8	RPS8	Homo sapiens	227.53	31.73	5	67
Q9H299	SH3 domain-binding glutamic acid-rich-like	SH3BGRL3	Homo sapiens	248.90	31.18	3	66
	protein 3
P19105	Myosin regulatory light chain 12A	MYL12A	Homo sapiens	243.16	39.18	5	66
O60701	UDP-glucose 6-dehydrogenase	UGDH	Homo sapiens	237.32	37.45	14	66
P52907	F-actin-capping-protein subunit alpha-1	CAPZA1	Homo sapiens	256.92	41.26	7	65
Q9NQC3	Reticulon-4	RTN4	Homo sapiens	252.94	9.65	6	65
P40925	Malate dehydrogenase, cytoplasmic	MDH1	Homo sapiens	225.55	24.55	6	65
Q15365	Poly(rC)-binding protein 1	PCBP1	Homo sapiens	225.32	32.02	6	65
P22626	Heterogeneous nuclear ribonucleoproteins A2/B1	HNRNPA2B1	Homo sapiens	223.70	30.88	9	65
P12236	ADP/ATP translocase 3	SLC25A6	Homo sapiens	178.86	28.52	8	65
Q14697	Neutral alpha-glucosidase AB	GANAB	Homo sapiens	308.38	13.67	9	64
P60842	Eukaryotic initiation factor 4A-I	EIF4A1	Homo sapiens	212.29	21.67	7	63
Q96D15	Reticulocalbin-3	RCN3	Homo sapiens	267.03	27.13	5	62
P30086	Phosphatidylethanolamine-binding protein 1	PEBP1	Homo sapiens	260.87	56.15	6	62
Q01518	Adenylyl cyclase-associated protein 1	CAP1	Homo sapiens	227.58	23.79	9	62
Q14974	Importin subunit beta-1	KPNB1	Homo sapiens	227.02	17.01	10	62
P55786	Puromycin-sensitive aminopeptidase	NPEPPS	Homo sapiens	202.84	13.93	10	62
P17655	Calpain-2 catalytic subunit	CAPN2	Homo sapiens	230.99	17.43	7	61
P04792	Heat shock protein beta-1	HSPB1	Homo sapiens	221.22	40.00	7	61
Q92928	Putative Ras-related protein Rab-1C	RAB1C	Homo sapiens	189.19	31.34	5	61
P23396	40S ribosomal protein S3	RPS3	Homo sapiens	182.60	52.26	10	61
P04843	Dolichyl-diphosphooligosaccharide--protein	RPN1	Homo sapiens	231.88	17.96	7	60
	glycosyltransferase subunit 1
P15121	Aldose reductase	AKR1B1	Homo sapiens	225.73	33.86	6	60
Q00839	Heterogeneous nuclear ribonucleoprotein U	HNRNPU	Homo sapiens	224.96	15.03	7	60
P35237	Serpin B6	SERPINB6	Homo sapiens	208.98	23.67	7	60
Q969G5	Protein kinase C delta-binding protein	PRKCDBP	Homo sapiens	200.31	31.03	7	60
P36578	60S ribosomal protein L4	RPL4	Homo sapiens	196.11	19.20	7	60
P17301	Integrin alpha-2	ITGA2	Homo sapiens	231.23	14.31	11	59
P00367	Glutamate dehydrogenase 1, mitochondrial	GLUD1	Homo sapiens	221.30	20.43	8	59
O00159	Unconventional myosin-Ic	MYO1C	Homo sapiens	204.23	15.71	12	59
P12956	X-ray repair cross-complementing protein 6	XRCC6	Homo sapiens	190.12	20.53	10	58
Q16658	Fascin	FSCN1	Homo sapiens	227.09	20.08	8	57
P22392	Nucleoside diphosphate kinase B	NME2	Homo sapiens	196.95	40.13	5	57
Q96CX2	BTB/POZ domain-containing protein KCTD12	KCTD12	Homo sapiens	173.33	14.77	4	57
P05141	ADP/ATP translocase 2	SLC25A5	Homo sapiens	157.41	25.17	7	57
P35527	Keratin, type I cytoskeletal 9	KRT9	Homo sapiens	274.80	30.82	12	56
Q8IUX7	Adipocyte enhancer-binding protein 1	AEBP1	Homo sapiens	245.03	10.71	8	56
P50990	T-complex protein 1 subunit theta	CCT8	Homo sapiens	199.96	19.15	8	56
P18085	ADP-ribosylation factor 4	ARF4	Homo sapiens	173.35	48.89	8	56
P02794	Ferritin heavy chain	FTH1	Homo sapiens	271.23	46.45	6	55
Q9Y696	Chloride intracellular channel protein 4	CLIC4	Homo sapiens	221.19	54.15	8	55
P49748	Very long-chain specific acyl-CoA	ACADVL	Homo sapiens	219.10	20.00	9	55
	dehydrogenase, mitochondrial
P98160	Basement membrane-specific heparan sulfate	HSPG2	Homo sapiens	208.59	5.01	14	55
	proteoglycan core protein
Q14108	Lysosome membrane protein 2	SCARB2	Homo sapiens	194.36	19.46	6	55
P62424	60S ribosomal protein L7a	RPL7A	Homo sapiens	182.21	29.70	7	55
P63241	Eukaryotic translation initiation factor 5A-1	EIF5A	Homo sapiens	232.00	30.52	5	54
P07108	Acyl-CoA-binding protein	DBI	Homo sapiens	224.72	50.57	3	54
P30153	Serine/threonine-protein phosphatase 2A 65	PPP2R1A	Homo sapiens	217.26	19.02	7	54
	kDa regulatory subunit A alpha isoform
P30050	60S ribosomal protein L12	RPL12	Homo sapiens	198.47	54.55	6	54
P16278	Beta-galactosidase	GLB1	Homo sapiens	198.03	11.23	6	54
P53621	Coatomer subunit alpha	COPA	Homo sapiens	169.83	10.78	10	54
P60033	CD81 antigen	CD81	Homo sapiens	252.20	25.00	3	53
P21810	Biglycan	BGN	Homo sapiens	223.44	24.73	8	53
P13667	Protein disulfide-isomerase A4	PDIA4	Homo sapiens	220.61	16.90	7	53
Q96KK5	Histone H2A type 1-H	HIST1H2AH	Homo sapiens	188.17	27.34	3	53
P37837	Transaldolase	TALDO1	Homo sapiens	169.53	14.24	4	53
P52565	Rho GDP-dissociation inhibitor 1	ARHGDIA	Homo sapiens	191.81	21.08	4	52
Q9P2E9	Ribosome-binding protein 1	RRBP1	Homo sapiens	182.04	11.63	10	52
P61204	ADP-ribosylation factor 3	ARF3	Homo sapiens	175.59	38.67	6	52
P68871	Hemoglobin subunit beta	HBB	Homo sapiens	167.11	53.74	6	52
Q99497	Protein DJ-1	PARK7	Homo sapiens	200.89	29.63	4	51
P21589	5′-nucleotidase	NT5E	Homo sapiens	178.39	26.31	11	51
P46821	Microtubule-associated protein 1B	MAP1B	Homo sapiens	172.54	8.35	13	51
Q15293	Reticulocalbin-1	RCN1	Homo sapiens	204.33	29.91	6	50
P07910	Heterogeneous nuclear ribonucleoproteins C1/C2	HNRNPC	Homo sapiens	147.42	18.30	5	50
P09651	Heterogeneous nuclear ribonucleoprotein A1	HNRNPA1	Homo sapiens	178.69	22.58	6	49
Q99715	Collagen alpha-1(XII) chain	COL12A1	Homo sapiens	178.41	5.91	12	49
P61247	40S ribosomal protein S3a	RPS3A	Homo sapiens	175.98	18.18	4	49
P10599	Thioredoxin	TXN	Homo sapiens	159.77	42.86	5	49
Q9Y6N5	Sulfide: quinone oxidoreductase, mitochondrial	SQRDL	Homo sapiens	168.71	28.00	10	48
P50395	Rab GDP dissociation inhibitor beta	GDI2	Homo sapiens	180.18	27.64	9	47
O43390	Heterogeneous nuclear ribonucleoprotein R	HNRNPR	Homo sapiens	178.95	8.53	4	47
Q01813	6-phosphofructokinase type C	PFKP	Homo sapiens	178.62	9.31	5	47
P07384	Calpain-1 catalytic subunit	CAPN1	Homo sapiens	157.93	12.61	6	47
P55209	Nucleosome assembly protein 1-like 1	NAP1L1	Homo sapiens	188.64	17.14	5	46
P47755	F-acting-capping protein subunit alpha-2	CAPZA2	Homo sapiens	173.61	28.67	6	46
P14550	Alcohol dehydrogenase [NADP(+)]	AKR1A1	Homo sapiens	169.26	13.54	4	46
P50609	Fibromodulin	FMOD	Rattus norvegicus	162.36	14.10	4	46
P24534	Elongation factor 1-beta	EEF1B2	Homo sapiens	195.28	20.44	3	45
P04080	Cystatin-B	CSTB	Homo sapiens	183.06	45.92	3	45
P14314	Glucosidase 2 subunit beta	PRKCSH	Homo sapiens	171.16	9.85	4	45
P46782	40S ribosomal protein S5 [Cleaved into: 40S	RPS5	Homo sapiens	157.22	18.14	3	45
	ribosomal protein S5, N-terminally processed]
P22105	Tenascin-X	TNXB	Homo sapiens	152.49	5.99	10	45
Q07020	60S ribosomal protein L18	RPL18	Homo sapiens	146.31	30.85	5	45
P50991	T-complex protein 1 subunit delta	CCT4	Homo sapiens	186.69	8.53	3	44
P62158	Calmodulin	CALM1	Homo sapiens	185.02	29.53	3	44
O95782	AP-2 complex subunit alpha-1	AP2A1	Homo sapiens	159.92	9.52	7	44
P17980	26S protease regulatory subunit 6A	PSMC3	Homo sapiens	155.58	29.61	8	44
P30044	Peroxiredoxin-5, mitochondrial	PRDX5	Homo sapiens	142.09	34.11	5	44
P48444	Coatomer subunit delta	ARCN1	Homo sapiens	128.85	9.00	4	44
P16403	Histone H1.2	HIST1H1C	Homo sapiens	127.74	15.49	4	44
P23219	Prostaglandin G/H synthase 1	PTGS1	Homo sapiens	174.36	12.85	5	43
P62826	GTP-binding nuclear protein Ran	RAN	Homo sapiens	156.07	39.35	7	43
P34932	Heat shock 70 kDa protein 4	HSPA4	Homo sapiens	142.63	12.88	7	43
P13645	Keratin, type I cytoskeletal 10	KRT10	Homo sapiens	134.26	20.03	9	43
P55084	Trifunctional enzyme subunit beta,	HADHB	Homo sapiens	160.26	19.20	7	42
	mitochondrial
P04899	Guanine nucleotide-binding protein G(I)	GNAI2	Homo sapiens	149.06	17.18	4	42
	subunit alpha-2
P11047	Laminin subunit gamma-1	LAMC1	Homo sapiens	141.74	6.53	7	42
P12882	Myosin-1	MYH1	Homo sapiens	159.40	5.98	8	41
P62701	40S ribosomal protein S4, X isoform	RPS4X	Homo sapiens	146.21	29.66	7	41
P51991	Heterogeneous nuclear ribonucleoprotein A3	HNRNPA3	Homo sapiens	140.08	17.20	5	41
Q15366	Poly(rC)-binding protein 2	PCBP2	Homo sapiens	126.95	14.79	4	41
P50914	60S ribosomal protein L14	RPL14	Homo sapiens	118.43	20.93	4	41
P05386	60S acidic ribosomal protein P1	RPLP1	Homo sapiens	214.61	51.75	2	40
P62906	60S ribosomal protein L10a	RPL10A	Homo sapiens	160.47	25.81	4	40
P10909	Clusterin	CLU	Homo sapiens	142.36	13.81	4	40
P15531	Nucleoside diphosphate kinase A	NME1	Homo sapiens	140.96	39.47	5	40
P27105	Erythrocyte band 7 integral membrane protein	STOM	Homo sapiens	138.94	24.65	5	40
Q12797	Aspartyl/asparaginyl beta-hydroxylase	ASPH	Homo sapiens	138.22	10.55	5	40
P32119	Peroxiredoxin-2	PRDX2	Homo sapiens	137.82	19.70	4	40
P39687	Acidic leucine-rich nuclear phosphoprotein 32	ANP32A	Homo sapiens	133.72	12.85	3	40
	family member A
P05556	Integrin beta-1	ITGB1	Homo sapiens	128.26	12.28	8	40
P27816	Microtubule-associated protein 4	MAP4	Homo sapiens	122.94	9.90	8	40
Q9Y6C2	EMILIN-1	EMILIN1	Homo sapiens	140.17	9.45	6	39
P08134	Rho-related GTP-binding protein RhoC	RHOC	Homo sapiens	138.88	32.64	5	39
P62979	Ubiquitin-40S ribosomal protein S27a	RPS27A	Homo sapiens	138.38	33.97	4	39
P19338	Nucleolin	NCL	Homo sapiens	136.47	13.66	8	39
P78371	T-complex protein 1 subunit beta	CCT2	Homo sapiens	160.95	27.29	10	38
P69905	Hemoglobin subunit alpha	HBA1;	Homo sapiens	143.65	48.59	4	38
P62277	40S ribosomal protein S13	RPS13	Homo sapiens	139.22	18.54	3	38
P17931	Galectin-3	LGALS3	Homo sapiens	137.26	28.40	6	38
P61586	Transforming protein RhoA	RHOA	Homo sapiens	134.81	38.86	6	38
Q13838	Spliceosome RNA helicase DDX39B	DDX39B	Homo sapiens	132.68	22.43	6	38
P41250	Glycine--tRNA ligase	GARS	Homo sapiens	120.83	10.42	5	38
P31949	Protein S100-A11	S100A11	Homo sapiens	110.54	25.71	3	38
Q16851	UTP--glucose-1-phosphate uridylyltransferase	UGP2	Homo sapiens	192.68	10.24	3	37
Q7KZF4	Staphylococcal nuclease domain-containing	SND1	Homo sapiens	139.09	13.52	8	37
	protein 1
P31939	Bifunctional purine biosynthesis protein PURH	ATIC	Homo sapiens	136.29	9.97	5	37
	[Includes:
	Phosphoribosylaminoimidazolecarboxamide
	formyltransferase]
P48047	ATP synthase subunit O, mitochondrial	ATP5O	Homo sapiens	127.11	44.60	6	37
P61106	Ras-related protein Rab-14	RAB14	Homo sapiens	127.06	30.70	4	37
O75874	Isocitrate dehydrogenase [NADP] cytoplasmic	IDH1	Homo sapiens	121.13	21.01	6	37
O43776	Asparagine--tRNA ligase, cytoplasmic	NARS	Homo sapiens	119.15	18.43	8	37
P56134	ATP synthase subunit f, mitochondrial	ATP5J2	Homo sapiens	117.20	25.53	2	37
P62249	40S ribosomal protein S16	RPS16	Homo sapiens	108.15	22.60	3	37
Q8NBS9	Thioredoxin domain-containing protein 5	TXNDC5	Homo sapiens	165.47	9.26	3	36
Q12905	Interleukin enhancer-binding factor 2	ILF2	Homo sapiens	139.27	11.28	3	36
Q9UBG0	C-type mannose receptor 2	MRC2	Homo sapiens	134.77	4.87	5	36
P06753	Tropomyosin alpha-3 chain	TPM3	Homo sapiens	111.21	13.38	5	36
P62942	Peptidyl-prolyl cis-trans isomerase FKBP1A	FKBP1A	Homo sapiens	158.27	29.63	3	35
Q07021	Complement component 1 Q subcomponent-	C1QBP	Homo sapiens	157.66	12.06	3	35
	binding protein, mitochondrial
P26599	Polypyrimidine tract-binding protein 1	PTBP1	Homo sapiens	155.40	14.12	5	35
P49411	Elongation factor Tu, mitochondrial	TUFM	Homo sapiens	138.71	15.27	4	35
P63010	AP-2 complex subunit beta	AP2B1	Homo sapiens	131.57	10.46	7	35
P08253	72 kDa type IV collagenase	MMP2	Homo sapiens	125.40	13.64	5	35
P13674	Prolyl 4-hydroxylase subunit alpha-1	P4HA1	Homo sapiens	121.33	14.98	6	35
P35908	Keratin, type II cytoskeletal 2 epidermal	KRT2	Homo sapiens	110.24	14.71	8	35
Q13162	Peroxiredoxin-4	PRDX4	Homo sapiens	109.40	16.97	4	35
P00441	Superoxide dismutase [Cu—Zn]	SOD1	Homo sapiens	201.61	32.47	4	34
P68036	Ubiquitin-conjugating enzyme E2 L3	UBE2L3	Homo sapiens	166.53	35.71	3	34
P07099	Epoxide hydrolase 1	EPHX1	Homo sapiens	151.01	21.76	7	34
O43399	Tumor protein D54	TPD52L2	Homo sapiens	134.95	31.07	4	34
P23634	Plasma membrane calcium-transporting	ATP2B4	Homo sapiens	133.50	3.46	3	34
	ATPase 4
P15559	NAD(P)H dehydrogenase [quinone] 1	NQO1	Homo sapiens	125.60	13.50	3	34
P51659	Peroxisomal multifunctional enzyme type 2	HSD17B4	Homo sapiens	122.52	14.67	7	34
P04259	Keratin, type II cytoskeletal 6B	KRT6B	Homo sapiens	118.21	20.21	10	34
P16615	Sarcoplasmic/endoplasmic reticulum calcium	ATP2A2	Homo sapiens	117.26	9.98	8	34
	ATPase 2
Q00325	Phosphate carrier protein, mitochondrial	SLC25A3	Homo sapiens	115.25	19.06	5	34
P62140	Serine/threonine-protein phosphatase PP1-	PPP1CB	Homo sapiens	115.19	18.96	5	34
	beta catalytic subunit
Q96TA1	Niban-like protein 1	FAM129B	Homo sapiens	113.43	9.65	5	34
P39019	40S ribosomal protein S19	RPS19	Homo sapiens	109.71	30.34	5	34
P62879	Guanine nucleotide-binding protein	GNB2	Homo sapiens	109.17	15.59	5	34
	G(I)/G(S)/G(T) subunit beta-2
P29692	Elongation factor 1-delta	EEF1D	Homo sapiens	108.71	13.17	3	34
P46783	40S ribosomal protein S10	RPS10	Homo sapiens	107.63	14.55	2	34
Q6NXT2	Histone H3.3C	H3F3C	Homo sapiens	89.79	11.85	2	34
P20810	Calpastatin	CAST	Homo sapiens	141.21	10.88	4	33
P17858	6-phosphofructokinase, liver type	PFKL	Homo sapiens	136.89	6.28	3	33
P62888	60S ribosomal protein L30	RPL30	Homo sapiens	131.49	51.30	4	33
Q16698	2,4-dienoyl-CoA reductase, mitochondrial	DECR1	Homo sapiens	121.31	13.73	3	33
P15880	40S ribosomal protein S2	RPS2	Homo sapiens	117.17	16.04	4	33
P18124	60S ribosomal protein L7	RPL7	Homo sapiens	116.55	16.13	3	33
P62269	40S ribosomal protein S18	RPS18	Homo sapiens	88.00	16.45	3	33
P16070	CD44 antigen	CD44	Homo sapiens	131.22	3.77	2	32
P49755	Transmembrane emp24 domain-containing	TMED10	Homo sapiens	124.36	22.37	5	32
	protein 10
Q9Y265	RuvB-like 1	RUVBL1	Homo sapiens	113.28	23.03	7	32
P61224	Ras-related protein Rap-1b	RAP1B	Homo sapiens	104.67	26.09	4	32
Q92688	Acidic leucine-rich nuclear phosphoprotein 32	ANP32B	Homo sapiens	103.10	21.12	4	32
	family member B
Q9BSJ8	Extended synaptotagmin-1	ESYT1	Homo sapiens	102.79	7.61	6	32
P78417	Glutathione S-transferase omega-1	GSTO1	Homo sapiens	95.20	19.92	4	32
P00325	Alcohol dehydrogenase 1B	ADH1B	Homo sapiens	125.69	20.00	7	31
P62244	40S ribosomal protein S15a	RPS15A	Homo sapiens	122.31	24.62	3	31
P47756	F-actin-capping protein subunit beta	CAPZB	Homo sapiens	117.28	20.22	4	31
P42785	Lysosomal Pro-X carboxypeptidase	PRCP	Homo sapiens	110.84	9.48	3	31
Q14103	Heterogeneous nuclear ribonucleoprotein D0	HNRNPD	Homo sapiens	94.78	13.52	4	31
Q01129	Decorin	DCN	Rattus norvegicus	90.56	11.86	4	31
P14866	Heterogeneous nuclear ribonucleoprotein L	HNRNPL	Homo sapiens	162.20	13.07	3	30
P06748	Nucleophosmin	NPM1	Homo sapiens	125.44	21.77	5	30
O60506	Heterogeneous nuclear ribonucleoprotein Q	SYNCRIP	Homo sapiens	112.80	14.77	6	30
Q96QK1	Vacuolar protein sorting-associated protein 35	VPS35	Homo sapiens	100.86	9.55	6	30
O00231	26S proteasome non-ATPase regulatory	PSMD11	Homo sapiens	95.64	8.77	3	30
	subunit 11
O14979	Heterogeneous nuclear ribonucleoprotein D-	HNRNPDL	Homo sapiens	94.56	9.76	3	30
	like
Q8WUM4	Programmed cell death 6-interacting protein	PDCD6IP	Homo sapiens	92.50	5.88	4	30
P59998	Actin-related protein 2/3 complex subunit 4	ARPC4	Homo sapiens	84.05	27.98	5	30
Q9H4M9	EH domain-containing protein 1	EHD1	Homo sapiens	129.70	14.42	5	29
P01033	Metalloproteinase inhibitor 1	TIMP1	Homo sapiens	127.55	22.22	3	29
P28066	Proteasome subunit alpha type-5	PSMA5	Homo sapiens	117.57	35.27	5	29
Q07960	Rho GTPase-activating protein 1	ARHGAP1	Homo sapiens	111.12	14.35	4	29
P02765	Alpha-2-HS-glycoprotein	AHSG	Homo sapiens	111.09	7.08	4	29
P10155	80 kDa SS-A/Ro ribonucleoprotein	TROVE2	Homo sapiens	101.48	4.46	2	29
P21399	Cytoplasmic aconitate hydratase	ACO1	Homo sapiens	92.08	9.67	6	29
P83731	60S ribosomal protein L24	RPL24	Homo sapiens	90.95	20.38	3	29
O00571	ATP-dependent RNA helicase DDX3X	DDX3X	Homo sapiens	90.65	10.12	5	29
P35232	Prohibitin	PHB	Homo sapiens	85.95	20.96	5	29
P46781	40S ribosomal protein S9	RPS9	Homo sapiens	79.12	25.77	6	29
P62829	60S ribosomal protein L23	RPL23	Homo sapiens	125.59	25.00	2	28
P25398	40S ribosomal protein S12	RPS12	Homo sapiens	123.76	31.82	3	28
Q08211	ATP-dependent RNA helicase A	DHX9	Homo sapiens	119.13	4.65	4	28
Q16181	Septin-7	41889	Homo sapiens	111.98	18.76	6	28
P62333	26S protease regulatory subunit 10B	PSMC6	Homo sapiens	103.91	14.91	4	28
P13010	X-ray repair cross-complementing protein 5	XRCC5	Homo sapiens	101.49	10.66	5	28
Q13492	Phosphatidylinositol-binding clathrin assembly	PICALM	Homo sapiens	100.88	4.91	2	28
	protein
P35606	Coatomer subunit beta	COPB2	Homo sapiens	93.08	9.05	6	28
P07996	Thrombospondin-1	THBS1	Homo sapiens	82.64	3.25	3	28
P29373	Cellular retinoic acid-binding protein 2	CRABP2	Homo sapiens	124.05	24.64	3	27
Q00341	Vigilin	HDLBP	Homo sapiens	109.99	4.89	4	27
P54709	Sodium/potassium-transporting ATPase	ATP1B3	Homo sapiens	101.67	17.20	3	27
	subunit beta-3
P49257	Protein ERGIC-53	LMAN1	Homo sapiens	98.91	14.51	3	27
Q06323	Proteasome activator complex subunit 1	PSME1	Homo sapiens	95.33	30.52	8	27
P61313	60S ribosomal protein L15	RPL15	Homo sapiens	89.98	12.75	2	27
P35268	60S ribosomal protein L22	RPL22	Homo sapiens	82.39	18.75	2	27
Q96HE7	ERO1-like protein alpha	ERO1L	Homo sapiens	113.81	13.25	4	26
P28838	Cytosol aminopeptidase	LAP3	Homo sapiens	110.86	12.33	4	26
Q9NVA2	Septin-11	41893	Homo sapiens	78.83	8.86	3	26
P20700	Lamin-B1	LMNB1	Homo sapiens	74.71	10.24	6	26
P51636	Caveolin-2	CAV2	Homo sapiens	111.42	29.63	3	25
P60866	40S ribosomal protein S20	RPS20	Homo sapiens	101.62	19.33	2	25
O14818	Proteasome subunit alpha type-7	PSMA7	Homo sapiens	98.96	20.56	3	25
Q9UBI6	Guanine nucleotide-binding protein	GNG12	Homo sapiens	97.88	31.94	2	25
	G(I)/G(S)/G(O) subunit gamma-12
P13647	Keratin, type II cytoskeletal 5	KRT5	Homo sapiens	86.02	13.90	8	25
O75915	PRA1 family protein 3	ARL6IP5	Homo sapiens	84.77	15.96	2	25
Q9Y2Q3	Glutathione S-transferase kappa 1	GSTK1	Homo sapiens	82.98	28.76	5	25
P62750	60S ribosomal protein L23a	RPL23A	Homo sapiens	78.51	15.38	2	25
P46776	60S ribosomal protein L27a	RPL27A	Homo sapiens	74.00	14.19	2	25
Q92743	Serine protease HTRA1	HTRA1	Homo sapiens	73.77	8.75	4	25
P17987	T-complex protein 1 subunit alpha	TCP1	Homo sapiens	73.10	6.29	3	25
P30048	Thioredoxin-dependent peroxide reductase,	PRDX3	Homo sapiens	121.36	23.44	3	24
	mitochondrial
P62263	40S ribosomal protein S14	RPS14	Homo sapiens	102.96	27.81	3	24
P07814	Bifunctional glutamate/proline--tRNA ligase	EPRS	Homo sapiens	96.07	4.30	4	24
P60953	Cell division control protein 42 homolog	CDC42	Homo sapiens	91.80	20.42	3	24
P51688	N-sulphoglucosamine sulphohydrolase	SGSH	Homo sapiens	90.99	8.37	3	24
Q13765	Nascent polypeptide-associated complex	NACA	Homo sapiens	90.13	12.56	2	24
	subunit alpha
P01893	Putative HLA class I histocompatibility	HLA-H	Homo sapiens	89.43	18.23	4	24
	antigen, alpha chain H
P52272	Heterogeneous nuclear ribonucleoprotein M	HNRNPM	Homo sapiens	83.38	9.73	5	24
P54920	Alpha-soluble NSF attachment protein	NAPA	Homo sapiens	81.50	22.37	5	24
P26373	60S ribosomal protein L13	RPL13	Homo sapiens	80.65	10.90	2	24
P26447	Protein S100-A4	S100A4	Homo sapiens	72.92	28.71	4	24
P39060	Collagen alpha-1(XVIII) chain [Cleaved into:	COL18A1	Homo sapiens	72.74	3.93	3	24
	Endostatin]
P78539	Sushi repeat-containing protein SRPX	SRPX	Homo sapiens	72.54	7.76	3	24
Q9UHD8	Septin-9	41891	Homo sapiens	70.31	10.07	4	24
P46926	Glucosamine-6-phosphate isomerase 1	GNPDA1	Homo sapiens	70.28	14.19	3	24
P61160	Actin-related protein 2	ACTR2	Homo sapiens	70.14	17.26	5	24
Q15185	Prostaglandin E synthase 3	PTGES3	Homo sapiens	117.88	26.25	3	23
O95373	Importin-7	IPO7	Homo sapiens	104.71	4.53	3	23
Q16881	Thioredoxin reductase 1, cytoplasmic	TXNRD1	Homo sapiens	90.34	8.17	3	23
P10620	Microsomal glutathione S-transferase 1	MGST1	Homo sapiens	87.69	27.10	3	23
Q13200	26S proteasome non-ATPase regulatory	PSMD2	Homo sapiens	78.79	10.24	6	23
	subunit 2
Q9H8H3	Methyltransferase-like protein 7A	METTL7A	Homo sapiens	77.76	12.30	2	23
P55795	Heterogeneous nuclear ribonucleoprotein H2	HNRNPH2	Homo sapiens	76.64	10.47	3	23
Q05682	Caldesmon	CALD1	Homo sapiens	76.04	3.66	3	23
P00966	Argininosuccinate synthase	ASS1	Homo sapiens	71.74	10.19	3	23
P51148	Ras-related protein Rab-5C	RAB5C	Homo sapiens	68.22	23.15	4	23
O75390	Citrate synthase, mitochondrial	CS	Homo sapiens	67.17	10.09	4	23
Q99829	Copine-1	CPNE1	Homo sapiens	66.73	4.66	2	23
Q92499	ATP-dependent RNA helicase DDX1	DDX1	Homo sapiens	112.53	7.43	3	22
Q08431	Lactadherin	MFGE8	Homo sapiens	106.96	8.53	2	22
Q15121	Astrocytic phosphoprotein PEA-15	PEA15	Homo sapiens	105.97	36.92	4	22
Q15181	Inorganic pyrophosphatase	PPA1	Homo sapiens	96.51	17.65	4	22
Q99623	Prohibitin-2	PHB2	Homo sapiens	91.02	21.07	5	22
P62280	40S ribosomal protein S11	RPS11	Homo sapiens	89.31	24.68	3	22
P04216	Thy-1 membrane glycoprotein	THY1	Homo sapiens	84.64	15.53	2	22
P06703	Protein S100-A6	S100A6	Homo sapiens	84.38	37.78	3	22
P12955	Xaa-Pro dipeptidase	PEPD	Homo sapiens	80.36	8.32	3	22
Q04760	Lactoylglutathione lyase	GLO1	Homo sapiens	70.31	14.67	2	22
Q8NHP8	Putative phospholipase B-like 2	PLBD2	Homo sapiens	69.68	8.49	4	22
P84103	Serine/arginine-rich splicing factor 3	SRSF3	Homo sapiens	69.34	14.02	2	22
P27635	60S ribosomal protein L10	RPL10	Homo sapiens	69.17	11.21	2	22
Q14152	Eukaryotic translation initiation factor 3 subunit	EIF3A	Homo sapiens	66.86	5.57	6	22
	A
P61088	Ubiquitin-conjugating enzyme E2 N	UBE2N	Homo sapiens	63.81	34.87	4	22
P62318	Small nuclear ribonucleoprotein Sm D3	SNRPD3	Homo sapiens	62.37	15.08	2	22
Q99460	26S proteasome non-ATPase regulatory	PSMD1	Homo sapiens	120.05	6.72	4	21
	subunit 1
P23142	Fibulin-1	FBLN1	Homo sapiens	84.86	13.23	4	21
P68402	Platelet-activating factor acetylhydrolase IB	PAFAH1B2	Homo sapiens	83.14	12.23	2	21
	subunit beta
P10619	Lysosomal protective protein	CTSA	Homo sapiens	82.10	5.00	2	21
P31943	Heterogeneous nuclear ribonucleoprotein H	HNRNPH1	Homo sapiens	80.87	7.57	2	21
P24752	Acetyl-CoA acetyltransferase, mitochondrial	ACAT1	Homo sapiens	78.01	9.84	3	21
P51571	Translocon-associated protein subunit delta	SSR4	Homo sapiens	74.54	24.86	3	21
Q15836	Vesicle-associated membrane protein 3	VAMP3	Homo sapiens	72.83	33.00	2	21
Q14847	LIM and SH3 domain protein 1	LASP1	Homo sapiens	67.61	17.62	4	21
Q99584	Protein S100-A13	S100A13	Homo sapiens	65.57	23.47	2	21
Q9Y277	Voltage-dependent anion-selective channel	VDAC3	Homo sapiens	62.19	12.01	3	21
	protein 3
P13797	Plastin-3	PLS3	Homo sapiens	62.18	14.76	7	21
P02533	Keratin, type I cytoskeletal 14	KRT14	Homo sapiens	61.78	16.74	7	21
P60981	Destrin	DSTN	Homo sapiens	60.55	20.00	3	21
Q15417	Calponin-3	CNN3	Homo sapiens	55.43	13.98	4	21
Q9BS26	Endoplasmic reticulum resident protein 44	ERP44	Homo sapiens	112.39	11.58	3	20
O94973	AP-2 complex subunit alpha-2	AP2A2	Homo sapiens	87.35	7.24	4	20
O14579	Coatomer subunit epsilon	COPE	Homo sapiens	84.58	19.48	5	20
O14773	Tripeptidyl-peptidase 1	TPP1	Homo sapiens	80.43	8.17	3	20
P13987	CD59 glycoprotein	CD59	Homo sapiens	70.28	18.75	2	20
P31937	3-hydroxyisobutyrate dehydrogenase,	HIBADH	Homo sapiens	69.39	7.14	2	20
	mitochondrial
P46778	60S ribosomal protein L21	RPL21	Homo sapiens	68.85	27.50	3	20
Q16363	Laminin subunit alpha-4	LAMA4	Homo sapiens	66.46	2.14	3	20
P15586	N-acetylglucosamine-6-sulfatase	GNS	Homo sapiens	60.55	6.88	3	20
P11940	Polyadenylate-binding protein 1	PABPC1	Homo sapiens	54.28	7.55	4	20
P30040	Endoplasmic reticulum resident protein 29	ERP29	Homo sapiens	54.08	14.18	3	20
Q9Y3F4	Serine-threonine kinase receptor-associated	STRAP	Homo sapiens	86.18	8.86	2	19
	protein
Q7L2H7	Eukaryotic translation initiation factor 3 subunit	EIF3M	Homo sapiens	74.90	15.78	4	19
	M
Q9Y3I0	tRNA-splicing ligase RtcB homolog	RTCB	Homo sapiens	73.48	13.47	4	19
Q9NVJ2	ADP-ribosylation factor-like protein 8B	ARL8B	Homo sapiens	72.73	19.89	2	19
P04632	Calpain small subunit 1	CAPNS1	Homo sapiens	72.63	16.42	3	19
P00568	Adenylate kinase isoenzyme 1	AK1	Homo sapiens	68.07	31.96	4	19
P42224	Signal transducer and activator of transcription	STAT1	Homo sapiens	67.24	9.73	5	19
	1-alpha/beta
Q02878	60S ribosomal protein L6	RPL6	Homo sapiens	66.73	15.63	3	19
O94979	Protein transport protein Sec31A	SEC31A	Homo sapiens	58.06	3.11	3	19
P45974	Ubiquitin carboxyl-terminal hydrolase 5	USP5	Homo sapiens	56.52	2.68	2	19
P38159	RNA-binding motif protein, X chromosome	RBMX	Homo sapiens	54.92	8.70	3	19
Q13557	Calcium/calmodulin-dependent protein kinase	CAMK2D	Homo sapiens	51.92	11.02	4	19
	type II subunit delta
P84074	Neuron-specific calcium-binding protein	HPCA	Homo sapiens	48.64	19.69	3	19
	hippocalcin
P40227	T-complex protein 1 subunit zeta	CCT6A	Homo sapiens	87.47	9.23	3	18
O43242	26S proteasome non-ATPase regulatory	PSMD3	Homo sapiens	79.83	7.68	3	18
	subunit 3
P30084	Enoyl-CoA hydratase, mitochondrial	ECHS1	Homo sapiens	78.24	13.10	2	18
O00410	Importin-5	IPO5	Homo sapiens	78.12	7.66	5	18
P46777	60S ribosomal protein L5	RPL5	Homo sapiens	71.13	23.91	5	18
Q15008	26S proteasome non-ATPase regulatory	PSMD6	Homo sapiens	69.46	7.20	2	18
	subunit 6
O15511	Actin-related protein 2/3 complex subunit 5	ARPC5	Homo sapiens	66.82	29.14	3	18
Q15436	Protein transport protein Sec23A	SEC23A	Homo sapiens	61.38	3.53	2	18
P19623	Spermidine synthase	SRM	Homo sapiens	59.81	21.19	4	18
Q02809	Procollagen-lysine,2-oxoglutarate 5-	PLOD1	Homo sapiens	58.99	5.91	3	18
	dioxygenase 1
P62081	40S ribosomal protein S7	RPS7	Homo sapiens	58.91	17.53	2	18
P25789	Proteasome subunit alpha type-4	PSMA4	Homo sapiens	47.57	13.03	2	18
Q9UBQ7	Glyoxylate reductase/hydroxypyruvate	GRHPR	Homo sapiens	84.31	17.38	3	17
	reductase
P20618	Proteasome subunit beta type-1	PSMB1	Homo sapiens	77.13	15.35	2	17
P21964	Catechol O-methyltransferase	COMT	Homo sapiens	76.86	8.49	2	17
O15460	Prolyl 4-hydroxylase subunit alpha-2	P4HA2	Homo sapiens	75.70	11.03	4	17
P62191	26S protease regulatory subunit 4	PSMC1	Homo sapiens	75.48	10.91	3	17
P63167	Dynein light chain 1, cytoplasmic	DYNLL1	Homo sapiens	72.59	37.08	2	17
P09429	High mobility group protein B1	HMGB1	Homo sapiens	72.42	20.93	3	17
Q13724	Mannosyl-oligosaccharide glucosidase	MOGS	Homo sapiens	70.23	7.53	4	17
P19367	Hexokinase-1	HK1	Homo sapiens	64.34	8.62	6	17
P23526	Adenosylhomocysteinase	AHCY	Homo sapiens	62.16	10.65	3	17
P39023	60S ribosomal protein L3	RPL3	Homo sapiens	58.60	17.87	5	17
P10301	Ras-related protein R-Ras	RRAS	Homo sapiens	55.83	12.84	2	17
Q96CW1	AP-2 complex subunit mu	AP2M1	Homo sapiens	55.49	12.87	4	17
P61353	60S ribosomal protein L27	RPL27	Homo sapiens	54.78	27.94	3	17
P11279	Lysosome-associated membrane glycoprotein	LAMP1	Homo sapiens	54.76	8.63	3	17
	1
Q14258	E3 ubiquitin/ISG15 ligase TRIM25	TRIM25	Homo sapiens	53.67	3.81	2	17
P62266	40S ribosomal protein S23	RPS23	Homo sapiens	53.61	13.99	2	17
P22695	Cytochrome b-c1 complex subunit 2,	UQCRC2	Homo sapiens	67.34	13.91	4	16
	mitochondrial
P36543	V-type proton ATPase subunit E 1	ATP6V1E1	Homo sapiens	65.25	6.19	2	16
P61604	10 kDa heat shock protein, mitochondrial	HSPE1	Homo sapiens	58.13	33.33	3	16
P40261	Nicotinamide N-methyltransferase	NNMT	Homo sapiens	57.15	18.18	3	16
Q63ZY3	KN motif and ankyrin repeat domain-	KANK2	Homo sapiens	56.52	4.70	3	16
	containing protein 2
P21266	Glutathione S-transferase Mu 3	GSTM3	Homo sapiens	53.50	20.44	4	16
O15260	Surfeit locus protein 4	SURF4	Homo sapiens	53.47	8.55	2	16
P05455	Lupus La protein	SSB	Homo sapiens	50.85	12.99	4	16
O15144	Actin-related protein 2/3 complex subunit 2	ARPC2	Homo sapiens	50.68	12.67	4	16
Q6NUM9	All-trans-retinol 13,14-reductase	RETSAT	Homo sapiens	49.04	4.92	2	16
O43143	Putative pre-mRNA-splicing factor ATP-	DHX15	Homo sapiens	47.67	2.89	2	16
	dependent RNA helicase DHX15
Q9Y3U8	60S ribosomal protein L36	RPL36	Homo sapiens	47.12	30.48	4	16
P42677	40S ribosomal protein S27	RPS27	Homo sapiens	46.35	25.00	2	16
P26640	Valine--tRNA ligase	VARS	Homo sapiens	44.08	3.64	4	16
P20908	Collagen alpha-1(V) chain	COL5A1	Homo sapiens	17.63	3.10	3	16
P35613	Basigin	BSG	Homo sapiens	47.61	8.31	2	15
P07711	Cathepsin L1	CTSL	Homo sapiens	46.98	8.41	2	15
O94905	Erlin-2	ERLIN2	Homo sapiens	45.32	10.91	3	15
P31948	Stress-induced-phosphoprotein 1	STIP1	Homo sapiens	41.60	9.39	4	15
P13473	Lysosome-associated membrane glycoprotein 2	LAMP2	Homo sapiens	41.41	7.07	3	15
P61970	Nuclear transport factor 2	NUTF2	Homo sapiens	67.00	33.86	2	14
P46439	Glutathione S-transferase Mu 5	GSTM5	Homo sapiens	65.44	11.93	2	14
Q8IWE2	Protein NOXP20	FAM114A1	Homo sapiens	63.09	5.68	2	14
Q92597	Protein NDRG1	NDRG1	Homo sapiens	61.64	8.88	2	14
O75533	Splicing factor 3B subunit 1	SF3B1	Homo sapiens	60.00	2.45	2	14
O75368	SH3 domain-binding glutamic acid-rich-like	SH3BGRL	Homo sapiens	53.79	37.72	3	14
	protein
P23246	Splicing factor, proline-and glutamine-rich	SFPQ	Homo sapiens	52.82	9.05	5	14
Q08380	Galectin-3-binding protein	LGALS3BP	Homo sapiens	51.25	7.52	3	14
O60763	General vesicular transport factor p115	USO1	Homo sapiens	48.56	3.85	3	14
P46977	Dolichyl-diphosphooligosaccharide--protein	STT3A	Homo sapiens	47.82	5.25	3	14
	glycosyltransferase subunit STT3A
Q15075	Early endosome antigen 1	EEA1	Homo sapiens	47.08	3.97	4	14
O95816	BAG family molecular chaperone regulator 2	BAG2	Homo sapiens	47.06	13.27	2	14
Q9NR45	Sialic acid sythase	NANS	Homo sapiens	46.21	15.88	3	14
P51858	Hepatoma-derived growth factor	HDGF	Homo sapiens	45.76	14.17	3	14
P00505	Aspartate aminotransferase, mitochondrial	GOT2	Homo sapiens	45.71	14.42	5	14
Q01105	Protein SET	SET	Homo sapiens	45.04	12.07	3	14
P61019	Ras-related protein Rab-2A	RAB2A	Homo sapiens	42.23	10.85	2	14
P19404	NADH dehydrogenase [ubiquinone]	NDUFV2	Homo sapiens	41.82	9.24	2	14
	flavoprotein 2, mitochondrial
P55884	Eukaryotic translation initiation factor 3 subunit B	EIF3B	Homo sapiens	40.54	5.90	4	14
Q13425	Beta-2-syntrophin	SNTB2	Homo sapiens	39.75	3.89	2	14
P54136	Arginine--tRNA ligase, cytoplasmic	RARS	Homo sapiens	38.18	6.97	4	14
P49773	Histidine triad nucleotide-binding protein 1	HINT1	Homo sapiens	83.21	34.92	2	13
P27695	DNA-(apurinic or apyrimidinic site) lyase	APEX1	Homo sapiens	63.88	22.96	4	13
Q53GQ0	Estradiol 17-beta-dehydrogenase 12	HSD17B12	Homo sapiens	54.92	9.62	2	13
Q5T9L3	Protein wntless homolog	WLS	Homo sapiens	54.68	8.13	3	13
O14880	Microsomal glutathione S-transferase 3	MGST3	Homo sapiens	54.48	23.03	2	13
P07954	Fumarate hydratase, mitochondrial	FH	Homo sapiens	53.05	7.45	2	13
Q8IZP2	Putative protein FAM10A4	ST13P4	Homo sapiens	51.61	17.08	3	13
O75131	Copine-3	CPNE3	Homo sapiens	50.89	10.80	3	13
P14854	Cytochrome c oxidase subunit 6B1	COX6B1	Homo sapiens	50.34	33.72	2	13
Q9UIJ7	GTP: AMP phosphotransferase AK3,	AK3	Homo sapiens	50.04	12.78	2	13
	mitochondrial
P23381	Tryptophan--tRNA ligase, cytoplasmic	WARS	Homo sapiens	50.02	5.73	2	13
Q12906	Interleukin enhancer-binding factor 3	ILF3	Homo sapiens	49.53	12.53	7	13
P27487	Dipeptidyl peptidase 4	DPP4	Homo sapiens	48.49	6.66	4	13
Q9BWD1	Acetyl-CoA acetyltransferase, cytosolic	ACAT2	Homo sapiens	47.57	14.61	3	13
P43243	Matrin-3	MATR3	Homo sapiens	46.57	6.02	3	13
P55010	Eukaryotic translation initiation factor 5	EIF5	Homo sapiens	45.49	9.98	3	13
P04179	Superoxide dismutase [Mn], mitochondrial	SOD2	Homo sapiens	43.52	22.97	4	13
P62330	ADP-ribosylation factor 6	ARF6	Homo sapiens	40.96	24.57	3	13
P41252	Isoleucine--tRNA ligase, cytoplasmic	IARS	Homo sapiens	38.88	4.75	5	13
P04062	Glucosylceramidase	GBA	Homo sapiens	36.15	7.09	3	13
P60900	Proteasome subunit alpha type-6	PSMA6	Homo sapiens	36.13	10.16	2	13
O43491	Band 4.1-like protein 2	EPB41L2	Homo sapiens	51.57	6.57	4	12
P10768	S-formylglutathione hydrolase	ESD	Homo sapiens	48.41	10.64	2	12
P36957	Dihydrolipoyllysine-residue	DLST	Homo sapiens	46.29	7.73	2	12
	succinyltransferase component of 2-
	oxoglutarate dehydrogenase complex,
	mitochondrial
P61254	60S ribosomal protein L26	RPL26	Homo sapiens	39.66	12.41	2	12
O15145	Actin-related protein 2/3 complex subunit 3	ARPC3	Homo sapiens	39.11	13.48	2	12
P08195	4F2 cell-surface antigen heavy chain	SLC3A2	Homo sapiens	38.01	4.13	2	12
Q9UKK3	Poly [ADP-ribose] polymerase 4	PARP4	Homo sapiens	37.80	1.68	2	12
Q03252	Lamin-B2	LMNB2	Homo sapiens	35.36	7.83	5	12
O95747	Serine/threonine-protein kinase OSR1	OXSR1	Homo sapiens	35.02	4.93	2	12
P39656	Dolichyl-diphosphooligosaccharide--protein	DDOST	Homo sapiens	33.93	12.28	5	12
	glycosyltransferase 48 kDa subunit
P78344	Eukaryotic translation initiation factor 4	EIF4G2	Homo sapiens	31.94	2.32	2	12
	gamma 2
Q6IBS0	Twinfilin-2	TWF2	Homo sapiens	46.21	15.19	3	11
P22087	rRNA 2′-O-methyltransferase fibrillarin	FBL	Homo sapiens	45.97	9.35	2	11
Q96KP4	Cytosolic non-specific dipeptidase	CNDP2	Homo sapiens	44.17	6.74	2	11
P09622	Dihydrolipoyl dehydrogenase, mitochondrial	DLD	Homo sapiens	42.94	6.48	2	11
Q99798	Aconitate hydratase, mitochondrial	ACO2	Homo sapiens	42.91	7.18	3	11
P04040	Catalase	CAT	Homo sapiens	42.83	11.20	4	11
Q07666	KH domain-containing, RNA-binding, signal	KHDRBS1	Homo sapiens	42.69	7.67	2	11
	transduction-associated protein 1
P07942	Laminin subunit beta-1	LAMB1	Homo sapiens	41.56	2.07	2	11
P00390	Glutathione reductase mitochondrial	GSR	Homo sapiens	39.02	6.32	2	11
Q9Y371	Endophilin-B1	SH3GLB1	Homo sapiens	38.47	6.85	2	11
P62195	26S protease regulatory subunit 8	PSMC5	Homo sapiens	37.79	12.07	3	11
Q9BWM7	Sideroflexin-3	SFXN3	Homo sapiens	35.68	11.69	3	11
P00403	Cytochrome c oxidase subunit 2	MT-CO2	Homo sapiens	35.42	11.45	2	11
O43615	Mitochondrial import inner membrane	C3	Homo sapiens	33.97	5.09	2	11
	translocase subunit TIM44
P30046	D-dopachrome decarboxylase	TIMM44	Homo sapiens	33.74	19.49	2	11
Q32P28	Prolyl 3-hydroxylase 1	DDT	Homo sapiens	32.20	3.40	2	11
P30740	Leukocyte elastase inhibitor	LEPRE1	Homo sapiens	31.82	6.60	2	11
Q9ULV4	Coronin-1C	SERPINB1	Homo sapiens	30.15	4.85	2	11
O60488	Long-chain-fatty-acid--CoA ligase 4	CORO1C	Homo sapiens	29.03	4.36	2	11
P01026	Complement C3 [Cleaved into: Complement	ACSL4	Rattus norvegicus	34.28	1.74	2	11
	C3 beta chain; Complement C3 alpha chain]
Q16643	Drebrin	DBN1	Homo sapiens	48.88	8.63	3	10
Q02952	A-kinase anchor protein 12	AKAP12	Homo sapiens	48.74	6.51	5	10
P28161	Glutathione S-transferase Mu 2	GSTM2	Homo sapiens	47.15	33.03	5	10
O75367	Core histone macro-H2A.1	H2AFY	Homo sapiens	42.46	15.59	3	10
O14980	Exportin-1	XPO1	Homo sapiens	42.26	6.35	4	10
P38606	V-type proton ATPase catalytic subunit A	ATP6V1A	Homo sapiens	40.66	8.91	3	10
O00151	PDZ and LIM domain protein 1	PDLIM1	Homo sapiens	39.15	15.20	3	10
P14868	Aspartate--tRNA ligase, cytoplasmic	DARS	Homo sapiens	37.60	7.98	3	10
Q13418	Integrin-linked protein kinase	ILK	Homo sapiens	37.04	9.73	3	10
Q14195	Dihydropyrimidinase-related protein 3	DPYSL3	Homo sapiens	36.24	19.12	6	10
P49589	Cysteine--tRNA ligase, cytoplasmic	CARS	Homo sapiens	34.54	4.68	2	10
P18621	60S ribosomal protein L17	RPL17	Homo sapiens	34.48	13.59	3	10
P06756	Integrin alpha-V	ITGAV	Homo sapiens	34.31	7.35	4	10
O75351	Vacuolar protein sorting-associated protein 4B	VPS4B	Homo sapiens	32.55	6.98	2	10
P02786	Transferrin receptor protein 1	TFRC	Homo sapiens	31.04	2.89	2	10
Q9NQW7	Xaa-Pro aminopeptidase 1	XPNPEP1	Homo sapiens	30.08	4.82	2	10
Q99961	Endophilin-A2	SH3GL1	Homo sapiens	30.00	12.23	3	10
P34897	Serine hydroxymethyltransferase,	SHMT2	Homo sapiens	29.51	7.54	3	10
	mitochondrial
P06865	Beta-hexosaminidase subunit alpha	HEXA	Homo sapiens	29.46	6.24	3	10
Q13263	Transcription intermediary factor 1-beta	TRIM28	Homo sapiens	26.56	5.87	3	10
Q02543	60S ribosomal protein L18a	RPL18A	Homo sapiens	25.80	9.66	2	10
P16401	Histone H1.5	HIST1H1B	Homo sapiens	25.79	10.18	2	10
P48643	T-complex protein 1 subunit epsilon	CCT5	Homo sapiens	40.56	15.71	5	9
P48147	Prolyl endopeptidase	PREP	Homo sapiens	36.97	5.49	2	9
P61769	Beta-2-microglobulin [Cleaved into: Beta-2-	B2M	Homo sapiens	35.59	35.29	3	9
	microglobulin form pl 5.3]
Q13228	Selenium-binding protein I	SELENBP1	Homo sapiens	34.80	11.65	4	9
P09960	Leukotriene A-4 hydrolase	LTA4H	Homo sapiens	34.51	6.71	3	9
Q92882	Osteoclast-stimulating factor 1	OSTF1	Homo sapiens	33.24	17.29	2	9
P43490	Nicotinamide phosphoribosyltransferase	NAMPT	Homo sapiens	32.14	8.35	2	9
P12268	Inosine-5′-monophosphate dehydrogenase 2	IMPDH2	Homo sapiens	31.91	5.84	2	9
P22102	Trifunctional purine biosynthetic protein	GART	Homo sapiens	31.74	2.67	2	9
	adenosine-3 [Includes: Phosphoribosylamine--
	glycine ligase]
P13804	Electron transfer flavoprotein subunit alpha,	ETFA	Homo sapiens	29.91	13.81	3	9
	mitochondrial
P62714	Serine/threonine-protein phosphatase 2A	PPP2CB	Homo sapiens	29.25	16.50	3	9
	catalytic subunit beta isoform
P13798	Acylamino-acid-releasing enzyme	APEH	Homo sapiens	29.20	4.78	2	9
O95336	6-phosphogluconolactonase	PGLS	Homo sapiens	29.02	17.05	3	9
Q14112	Nidogen-2	NID2	Homo sapiens	28.75	2.76	3	9
P20073	Annexin A7	ANXA7	Homo sapiens	28.58	8.40	3	9
Q9NZ08	Endoplasmic reticulum aminopeptidase 1	ERAP1	Homo sapiens	27.64	4.36	3	9
Q13561	Dynactin subunit 2	DCTN2	Homo sapiens	27.33	8.23	2	9
Q9UEY8	Gamma-adducin	ADD3	Homo sapiens	26.05	7.51	4	9
Q9UHL4	Dipeptidyl peptidase 2	DPP7	Homo sapiens	25.73	4.47	2	9
Q92841	Probable ATP-dependent RNA helicase	DDX17	Homo sapiens	25.28	6.17	4	9
	DDX17
P62910	60S ribosomal protein L32	RPL32	Homo sapiens	24.56	27.41	3	9
Q16401	26S proteasome non-ATPase regulatory	PSMD5	Homo sapiens	30.76	9.33	3	8
	subunit 5
Q02818	Nucleobindin-1	NUCB1	Homo sapiens	30.34	6.29	2	8
Q9NTK5	Obg-like ATPase 1	OLA1	Homo sapiens	30.03	8.33	2	8
Q9UHG3	Prenylcysteine oxidase 1	PCYOX1	Homo sapiens	29.06	7.33	2	8
P08473	Neprilysin	MME	Homo sapiens	26.86	10.40	5	8
P84098	60S ribosomal protein L19	RPL19	Homo sapiens	26.10	13.27	2	8
P19823	Inter-apha-trypsin inhibitor heavy chain H2	ITIH2	Homo sapiens	25.74	2.75	2	8
P30626	Sorcin	SRI	Homo sapiens	24.78	11.11	2	8
Q9NYL9	Tropomodulin-3	TMOD3	Homo sapiens	24.64	7.10	2	8
P53999	Activated RNA polymerase II transcriptional	SUB1	Homo sapiens	23.69	18.90	2	8
	coactivator p15
Q9UJ70	N-acetyl-D-glucosamine kinase	NAGK	Homo sapiens	23.68	11.92	3	8
P62851	40S ribosomal protein S25	RPS25	Homo sapiens	23.28	16.00	2	8
P0CW22	40S ribosomal protein S17-like	RPS17L	Homo sapiens	23.16	15.56	2	8
P80303	Nucleobindin-2	NUCB2	Homo sapiens	22.85	5.95	2	8
P28845	Corticosteroid 11-beta-dehydrogenase	HSD11B1	Homo sapiens	22.66	10.96	2	8
	isozyme 1
O43237	Cytoplasmic dynein 1 light intermediate chain	DYNC1LI2	Homo sapiens	22.33	5.08	2	8
	2
Q9NYU2	UDP-glucose: glycoprotein glucosyltransferase 1	UGGT1	Homo sapiens	22.30	3.79	4	8
Q9Y4L1	Hypoxia up-regulated protein 1	HYOU1	Homo sapiens	20.71	2.80	2	8
P05023	Sodium/potassium-transporting ATPase	ATP1A1	Homo sapiens	20.70	3.52	3	8
	subunit alpha-1
F54727	UV excision repair protein RAD23 homolog B	RAD23B	Homo sapiens	30.83	7.09	2	7
O75955	Flotillin-1	FLOT1	Homo sapiens	29.59	6.79	2	7
P49591	Serine--tRNA ligase, cytoplasmic	SARS	Homo sapiens	29.21	11.67	3	7
P46063	ATP-dependent DNA helicase Q1	RECQL	Homo sapiens	28.61	5.08	2	7
P53007	Tricarboxylate transport protein, mitochondrial	SLC25A1	Homo sapiens	25.56	11.25	3	7
Q9UQ80	Proliferation-associated protein 2G4	PA2G4	Homo sapiens	25.34	8.38	2	7
O94855	Protein transport protein Sec24D	SEC24D	Homo sapiens	23.19	2.71	2	7
P05091	Aldehyde dehydrogenase, mitochondrial	ALDH2	Homo sapiens	23.16	13.73	4	7
Q15847	Adipogenesis regulatory factor	ADIRF	Homo sapiens	22.08	59.21	2	7
Q9UM54	Unconventional myosin-VI	MYO6	Homo sapiens	21.91	2.16	2	7
P42765	3-ketoacyl-CoA thiolase, mitochondrial	ACAA2	Homo sapiens	21.44	7.81	2	7
Q9NR31	GTF-binding protein SAR1a	SAR1A	Homo sapiens	21.19	21.72	3	7
P99999	Cytochrome c	CYCS	Homo sapiens	20.83	24.76	2	7
Q9HC38	Glyoxelase domain-containing protein 4	GLOD4	Homo sapiens	20.63	7.35	2	7
Q99832	T-complex protein 1 subunit eta	CCT7	Homo sapiens	20.23	4.79	2	7
Q5SSJ5	Heterochromatin protein 1-binding protein 3	HP1BP3	Homo sapiens	19.85	4.70	2	7
P36405	ADP-ribosylation factor-like protein 3	ARL3	Homo sapiens	19.10	12.64	2	7
P50281	Matrix metalloproteinase-14	MMP14	Homo sapiens	19.03	4.12	2	7
P40429	60S ribosomal protein L13a	RPL13A	Homo sapiens	18.87	8.87	2	7
Q13510	Acid ceramidase	ASAH1	Homo sapiens	30.44	10.13	2	6
Q99714	3-hydroxyacyl-CoA dehydrogenase type-2	HSD17B10	Homo sapiens	25.28	16.48	2	6
Q13423	NAD(P) transhydrogenase, mitochondrial	NNT	Homo sapiens	24.97	4.51	3	6
P29966	Myristoylated alanine-rich C-kinase substrate	MARCKS	Homo sapiens	24.68	25.00	3	6
O43681	ATPase ASNA1	ASNA1	Homo sapiens	22.72	8.91	2	6
P05198	Eukaryotic translation initiation factor 2 subunit	EIF2S1	Homo sapiens	21.96	14.92	3	6
	1
P55036	26S proteasome non-ATPase regulatory	PSMD4	Homo sapiens	21.86	11.94	3	6
	subunit 4
P26368	Splicing factor U2AF 65 kDa subunit	U2AF2	Homo sapiens	21.60	6.11	2	6
P49189	4-trimethylaminobutyraldehyde	ALDH9A1	Homo sapiens	20.13	5.26	2	6
	dehydrogenase
P09936	Ubiquitin carboxyl-terminal hydrolase isozyme	UCHL1	Homo sapiens	19.71	14.35	2	6
	L1
Q02218	2-oxoglutarate dehydrogenase, mitochondrial	OGDH	Homo sapiens	19.31	5.38	4	6
Q9UNM6	26S proteasome non-ATPase regulatory	PSMD13	Homo sapiens	18.66	9.57	3	6
	subunit 13
P09110	3-ketoacyl-CoA thiolase, peroxisomal	ACAA1	Homo sapiens	18.52	7.78	2	6
Q9P2J5	Leucine--tRNA ligase, cytoplasmic	LARS	Homo sapiens	17.83	1.79	2	6
P10515	Dihydrolipoyllysine-residue acetyltransferase	DLAT	Homo sapiens	17.46	4.48	2	6
	component of pyruvate dehydrogenase
	complex, mitochondrial
Q9Y262	Eukaryotic translation initiation factor 3 subunit	EIF3L	Homo sapiens	17.30	3.55	2	6
	L
P55735	Protein SEC13 homolog	SEC13	Homo sapiens	17.22	13.35	3	6
P25788	Proteasome subunit alpha type-3	PSMA3	Homo sapiens	17.13	14.12	3	6
P32969	60S ribosomal protein L9	RPL9	Homo sapiens	16.93	11.46	3	6
Q16891	Mitochondrial inner membrane protein	IMMT	Homo sapiens	16.26	4.35	2	6
Q16531	DNA damage-binding protein 1	DDB1	Homo sapiens	24.08	3.07	2	5
P49419	Alpha-aminoadipic semialdehyde	ALDH7A1	Homo sapiens	19.67	5.94	2	5
	dehydrogenase
P05413	Fatty acid-binding protein, heart	FABP3	Homo sapiens	19.47	31.58	3	5
P80723	Brain acid soluble protein 1	BASP1	Homo sapiens	19.41	18.50	2	5
Q92905	COP9 signalsome complex subunit 5	COPS5	Homo sapiens	17.81	6.89	2	5
P54578	Ubiquitin carboxyl-terminal hydrolase 14	USP14	Homo sapiens	16.08	5.87	2	5
Q12884	Seprase	FAP	Homo sapiens	15.64	2.76	2	5
O9P0L0	Vesicle-associated membrane protein-	VAPA	Homo sapiens	15.58	11.24	2	5
	associated protein A
P10253	Lysosomal alpha-glucosidase	GAA	Homo sapiens	15.19	3.05	2	5
Q99439	Calponin-2	CNN2	Homo sapiens	15.15	12.30	3	5
Q969H8	UPF0556 protein C19orf10	C19ORF10	Homo sapiens	14.99	15.03	2	5
P00491	Purine nucleoside phosphorylase	PNP	Homo sapiens	14.91	9.69	2	5
Q9NSE4	Isoleucine--tRNA ligase, mitochondrial	IARS2	Homo sapiens	14.69	3.16	3	5
Q09028	Histone-binding protein RBBP4	RBBP4	Homo sapiens	14.18	4.94	2	5
P52630	Signal transducer and activator of transcription 2	STAT2	Homo sapiens	13.75	2.82	2	5
P08648	Integrin alpha-5	ITGA5	Homo sapiens	12.87	2.00	2	5
P06454	Prothymosin alpha [Cleaved into: Prothymosin	PTMA	Homo sapiens	12.15	20.72	2	5
	alpha, N-terminally processed; Thymosin
	alpha-1]
P43304	Glycerol-3-phosphate dehydrogenase,	GPD2	Homo sapiens	18.91	4.81	2	4
	mitochondrial
Q9ULZ3	Apoptosis-associated speck-like protein	PYCARD	Homo sapiens	17.85	12.82	2	4
	containing a CARD
O00154	Cytosolic acyl coenzyme A thioester hydrolase	ACOT7	Homo sapiens	17.76	9.47	2	4
P21291	Cysteine and glycine-rich protein 1	CSRP1	Homo sapiens	16.70	20.21	2	4
P27701	CD82 antigen	CDB2	Homo sapiens	15.88	10.49	2	4
P11586	C-1-tetrahydrofolate synthase, cytoplasmic	MTHFD1	Homo sapiens	15.19	5.24	3	4
Q6IAAB	Ragulator complex protein LAMTOR1	LAMTOR1	Homo sapiens	15.17	15.53	2	4
O15230	Laminin subunit alpha-5	LAMA5	Homo sapiens	14.56	0.97	2	4
Q6P2Q9	Pre-mRNA-processing-splicing factor 8	PRPF8	Homo sapiens	14.44	1.24	2	4
O60437	Periplakin	PPL	Homo sapiens	14.03	3.13	3	4
Q96FQ6	Protein S100-A16	S100A16	Homo sapiens	13.82	22.33	2	4
O00303	Eukaryotic translation initiation factor 3 subunit	EIF3F	Homo sapiens	13.71	7.28	2	4
	F
P43487	Ran-specific GTPase-activating protein	RANBP1	Homo sapiens	13.26	16.92	2	4
O00425	Insulin-like growth factor 2 mRNA-binding	IGF2BP3	Homo sapiens	12.21	5.18	2	4
	protein 3
Q9Y230	RuvB-like 2	RUVBL2	Homo sapiens	12.00	9.50	3	4
P55060	Exportin 2	CSE1L	Homo sapiens	11.83	3.40	2	4
P20674	Cytochrome c oxidase subunit 5A,	COX5A	Homo sapiens	11.58	16.00	2	4
	mitochondrial
P35611	Alpha-adducin	ADD1	Homo sapiens	11.18	4.21	2	4
P10644	cAMP-dependent protein kinase type I-alpha	PRKAR1A	Homo sapiens	11.12	9.19	2	4
	regulatory subunit
Q9NUQ9	Protein FAM49B	FAM49B	Homo sapiens	10.88	8.64	2	4
O95302	Peptidyl-prolyl cis-trans isomerase FKBP9	FKBP9	Homo sapiens	10.34	4.56	2	4
P63220	40S ribosomal protein S21	RPS21	Homo sapiens	10.11	22.89	2	4
P49720	Proteasome subunit beta type-3	PSMB3	Homo sapiens	12.83	15.12	2	3
P62491	Ras-related protein Rab-11A	RAB11A	Homo sapiens	12.02	11.11	2	3
Q2TAA2	Isoamyl acetate-hydrolyzing esterase 1	IAH1	Homo sapiens	12.02	10.89	2	3
	homolog
Q08257	Quinone oxidoreductase	CRYZ	Homo sapiens	11.77	10.64	2	3
O75694	Nuclear pore complex protein Nup155	NUP155	Homo sapiens	11.59	2.23	2	3
Q14247	Src substrate cortactin	CTTN	Homo sapiens	10.48	4.73	2	3
Q8IVL6	Prolyl 3-hydroxylase 3	LEPREL2	Homo sapiens	10.19	4.21	2	3
P00492	Hypoxanthine-guanine	HPRT1	Homo sapiens	9.71	10.55	2	3
	phosphoribosyltransferase
Q9P0J0	NADH dehydrogenase [ubiquinone] 1 alpha	NDUFA13	Homo sapiens	9.66	9.72	2	3
	subcomplex subunit 13
Q86UP2	Kinectin	KTN1	Homo sapiens	9.51	2.21	2	3
O75489	NADH dehydrogenase [ubiquinone] iron-sulfur	NDUFS3	Homo sapiens	9.34	15.53	3	3
	protein 3, mitochondrial
Q9UL46	Proteasome activator complex subunit 2	PSME2	Homo sapiens	8.76	12.13	2	3
Q13057	Bifunctional coenzyme A synthase	COASY	Homo sapiens	8.68	4.96	2	3
P48163	NADP-dependent malic enzyme	ME1	Homo sapiens	8.67	5.24	2	3
Q13488	V-type proton ATPase 116 kDa subunit a	TCIRG1	Homo sapiens	8.61	2.65	2	3
	isoform 3
Q9H845	Acyl-CoA dehydrogenase family member 9,	ACAD9	Homo sapiens	8.54	4.35	2	3
	mitochondrial
P60228	Eukaryotic translation initiation factor 3 subunit E	EIF3E	Homo sapiens	8.48	4.94	2	3
Q15758	Neutral amino acid transporter B(0)	SLC1A5	Homo sapiens	7.94	4.44	2	3
Q96AC1	Ferritin family homolog 2	FERMT2	Homo sapiens	7.61	3.82	2	3
P22234	Multifuntional protein ADE2 [Includes:	PAICS	Homo sapiens	7.51	6.59	2	3
	Phosphoribosylaminoimidazole-
	succinocarboxamide synthase]
P14923	Junction plakoglobin	JUP	Homo sapiens	9.12	3.76	2	2
Q687X5	Metalloreductase STEAP4	STEAP4	Homo sapiens	8.11	7.19	2	2
P18859	ATP synthase-coupling factor 6, mitochondrial	ATP5J	Homo sapiens	7.62	26.85	2	2
Q14766	Latent-transforming growth factor beta-binding	LTBP1	Homo sapiens	7.23	1.69	2	2
	protein 1
Q6YHK3	CD109 antigen	CD109	Homo sapiens	7.08	1.87	2	2
O14737	Programmed cell death protein 5	PDCD5	Homo sapiens	6.87	19.20	2	2
Q9Y3B3	Transmembrane emp24 domain-containing	TMED7	Homo sapiens	6.13	11.16	2	2
	protein 7
Q9Y240	C-type lectin domain family 11 member A	CLEC11A	Homo sapiens	6.11	8.98	2	2
P48735	Isocitrate dehydrogenase [NADP],	IDH2	Homo sapiens	6.06	5.97	2	2
	mitochondrial
P42704	Leucine-rich PPR motif-containing protein,	LRPPRC	Homo sapiens	5.73	2.22	2	2
	mitochondrial
Q15819	Ubiquitin-conjugating enzyme E2 variant 2	UBE2V2	Homo sapiens	5.19	13.79	2	2
Q9C0C2	182 kDa tankyrase-1-binding protein	TNKS1BP1	Homo sapiens	4.83	1.56	2	2
Native VF mucosa
P02768	Serum albumin	ALB	Homo sapiens	9377.55	78.49	52	2323
P02452	Collagen alpha-1(I) chain	COL1A1	Homo sapiens	1727.69	43.65	37	2130
P12109	Collagen alpha-1(VI) chain	COL6A1	Homo sapiens	3245.57	49.22	34	1464
P12111	Collagen alpha-3(VI) chain	COL6A3	Homo sapiens	5535.48	37.46	92	1447
P08123	Collagen alpha-2(I) chain	COL1A2	Homo sapiens	1753.82	37.70	31	1416
P02461	Collagen alpha-1(III) chain	COL3A1	Homo sapiens	1145.04	33.77	30	1365
P13647	Keratin, type II cytoskeletal 5	KRT5	Homo sapiens	4377.87	61.36	49	1272
Q9UKX2	Myosin-2	MYH2	Homo sapiens	5300.08	60.79	135	1228
P35555	Fibrillin-1	FBN1	Homo sapiens	4497.74	38.56	75	1166
P02675	Fibrinogen beta chain	FGB	Homo sapiens	3486.23	70.67	30	1070
P12882	Myosin-1	MYH1	Homo sapiens	4537.67	58.17	125	1060
P13646	Keratin, type I cytoskeletal 13	KRT13	Homo sapiens	3623.18	63.97	34	1001
P02538	Keratin, type II cytoskeletal 6A	KRT6A	Homo sapiens	3925.12	63.48	46	999
Q09666	Neuroblast differentiation-associated protein	AHNAK	Homo sapiens	3348.49	37.64	95	933
	AHNAK
P35579	Myosin-9	MYH9	Homo sapiens	3935.56	49.29	81	909
P22105	Tenascin-X	TNXB	Homo sapiens	3631.59	35.42	90	902
P04259	Keratin, type II cytoskeletal 6B	KRT6B	Homo sapiens	3609.25	56.91	42	900
P01024	Complement C3	C3	Homo sapiens	3126.18	51.11	63	763
P02533	Keratin, type I cytoskeletal 14	KRT14	Homo sapiens	2848.68	71.19	35	756
P08727	Keratin, type I cytoskeletal 19	KRT19	Homo sapiens	2653.17	84.50	36	731
P15924	Desmoplakin	DSP	Homo sapiens	2903.43	39.36	84	725
P60709	Actin, cytoplasmic 1	ACTB	Homo sapiens	2698.92	64.00	18	696
Q15149	Plectin	PLEC	Homo sapiens	2779.52	29.76	97	696
P19013	Keratin, type II cytoskeletal 4	KRT4	Homo sapiens	2651.92	62.73	35	685
P02679	Fibrinogen gamma chain	FGG	Homo sapiens	2758.21	77.04	29	677
P68133	Actin, alpha skeletal muscle	ACTA1	Homo sapiens	2630.03	66.84	20	663
P01023	Alpha-2-macroglobulin	A2M	Homo sapiens	2609.50	51.76	51	660
P12110	Collagen alpha-2(VI) chain	COL6A2	Homo sapiens	1699.58	37.49	33	649
P68032	Actin, alpha cardiac muscle 1	ACTC1	Homo sapiens	2619.50	66.84	20	645
P12883	Myosin-7	MYH7	Homo sapiens	2691.89	52.20	97	637
P02671	Fibrinogen alpha chain	FGA	Homo sapiens	1988.48	41.22	32	621
P08670	Vimentin	VIM	Homo sapiens	2279.55	71.46	37	596
Q8WZ42	Titin	TTN	Homo sapiens	2540.73	10.62	200	584
P02787	Serotransferrin	TF	Homo sapiens	2398.90	63.04	39	574
P01009	Alpha-1-antitrypsin	SERPINA1	Homo sapiens	2181.30	58.61	27	539
Q00610	Clathrin heavy chain 1	CLTC	Homo sapiens	2208.17	47.34	53	495
O43707	Alpha-actinin-4	ACTN4	Homo sapiens	2157.22	64.54	43	494
Q01546	Keratin, type II cytoskeletal 2 oral	KRT76	Homo sapiens	1104.99	14.73	12	492
P02545	Prelamin-A/C	LMNA	Homo sapiens	1738.37	54.07	37	473
P19012	Keratin, type I cytoskeletal 15	KRT15	Homo sapiens	1698.59	67.32	28	464
P07355	Annexin A2	ANXA2	Homo sapiens	1886.30	67.55	24	462
P68371	Tubulin beta-4B chain	TUBB4B	Homo sapiens	2035.19	72.58	23	462
P02751	Fibronectin	FN1	Homo sapiens	2046.04	41.74	58	458
P07437	Tubulin beta chain	TUBB	Homo sapiens	2039.92	72.75	23	455
P00738	Haptoglobin	HP	Homo sapiens	1785.78	61.58	24	453
P01011	Alpha-1-antichymotrypsin	SERPINA3	Homo sapiens	1446.96	50.35	18	425
P06576	ATP synthase subunit beta, mitochondrial	ATP5B	Homo sapiens	1859.52	66.16	23	410
P01857	Ig gamma-1 chain C region	IGHG1	Homo sapiens	1887.72	50.61	12	406
P00450	Ceruloplasmin	CP	Homo sapiens	1918.16	46.29	29	398
P01859	Ig gamma-2 chain C region	IGHG2	Homo sapiens	1801.70	51.53	11	392
P51888	Prolargin	PRELP	Homo sapiens	1226.46	47.12	15	387
P04083	Annexin A1	ANXA1	Homo sapiens	1651.02	64.45	21	382
P04406	Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	Homo sapiens	1670.21	73.73	17	376
P68363	Tubulin alpha-1B chain	TUBA1B	Homo sapiens	1568.91	68.96	18	350
Q71U36	Tubulin alpha-1A chain	TUBA1A	Homo sapiens	1565.01	68.96	18	349
P08779	Keratin, type I cytoskeletal 16	KRT16	Homo sapiens	1255.62	52.64	25	346
Q04695	Keratin, type I cytoskeletal 17	KRT17	Homo sapiens	1278.10	79.63	32	345
P05787	Keratin, type II cytoskeletal 8	KRT8	Homo sapiens	1167.17	48.03	24	342
P21333	Filamin-A	FLNA	Homo sapiens	1423.87	35.17	54	341
Q13885	Tubulin beta-2A chain	TUBB2A	Homo sapiens	1387.87	56.40	18	341
Q9BQE3	Tubulin alpha-1C chain	TUBA1C	Homo sapiens	1498.39	69.27	18	332
P14618	Pyruvate kinase isozymes M1/M2	PKM	Homo sapiens	1344.45	68.17	28	328
Q15063	Periostin	POSTN	Homo sapiens	1387.00	43.54	25	327
P58107	Epiplakin	EPPK1	Homo sapiens	1409.85	46.90	45	321
P07585	Decorin	DCN	Homo sapiens	1226.95	49.30	15	317
P14923	Junction plakoglobin	JUP	Homo sapiens	1478.56	55.17	28	314
P35609	Alpha-actinin-2	ACTN2	Homo sapiens	1213.25	55.48	38	305
P20774	Mimecan	OGN	Homo sapiens	1230.96	44.30	13	301
P21810	Biglycan	BGN	Homo sapiens	1169.94	51.09	14	293
P12814	Alpha-actinin-1	ACTN1	Homo sapiens	1088.32	34.87	27	291
P00488	Coagulation factor XIII A chain	F13A1	Homo sapiens	1224.31	40.57	21	291
P08107	Heat shock 70 kDa protein 1A/1B	HSPA1A	Homo sapiens	1201.31	55.38	25	291
P01876	Ig alpha-1 chain C region	IGHA1	Homo sapiens	1328.87	50.42	11	291
P02788	Lactotransferrin	LTF	Homo sapiens	1000.06	67.61	39	283
P13639	Elongation factor 2	EEF2	Homo sapiens	998.65	37.18	24	279
Q13813	Spectrin alpha chain, non-erythrocytic 1	SPTAN1	Homo sapiens	1389.63	27.71	42	276
P02647	Apolipoprotein A-I	APOA1	Homo sapiens	1111.22	70.79	22	274
P01860	Ig gamma-3 chain C region	IGHG3	Homo sapiens	1182.07	45.09	11	273
P11142	Heat shock cognate 71 kDa protein	HSPA8	Homo sapiens	1142.75	39.16	21	272
P06899	Histone H2B type 1-J	HIST1H2BJ	Homo sapiens	740.33	40.48	5	271
P68871	Hemoglobin subunit beta	HBB	Homo sapiens	1083.28	88.44	12	271
P08758	Annexin A5	ANXA5	Homo sapiens	1003.25	56.56	17	268
P11021	78 kDa glucose-regulated protein	HSPA5	Homo sapiens	958.13	39.60	22	265
Q15582	Transforming growth factor-beta-induced	TGFBI	Homo sapiens	996.01	49.19	24	264
	protein Ig-h3
P69905	Hemoglobin subunit alpha	HBA1;	Homo sapiens	1419.48	83.80	8	262
P08603	Complement factor H	CFH	Homo sapiens	966.16	38.67	32	257
P46940	Ras GTPase-activating-like IQGAP1	IQGAP1	Homo sapiens	1173.73	26.55	28	253
P06732	Creatine kinase M-type	CKM	Homo sapiens	997.59	63.78	17	252
P02790	Hemopexin	HPX	Homo sapiens	1318.29	50.65	14	251
P20908	Collagen alpha-1(V) chain	COL5A1	Homo sapiens	159.61	3.97	4	248
P01834	Ig kappa chain C region	IGKC	Homo sapiens	998.31	80.19	6	243
P06396	Gelsolin	GSN	Homo sapiens	1156.49	32.99	15	241
P06733	Alpha-enolase	ENO1	Homo sapiens	813.18	60.37	18	239
Q02388	Collagen alpha-1(VII) chain	COL7A1	Homo sapiens	896.99	17.87	33	238
P55083	Microfibril-associated glycoprotein 4	MFAP4	Homo sapiens	1345.67	44.71	5	236
P51884	Lumican	LUM	Homo sapiens	756.06	37.87	11	235
P02763	Alpha-1-acid glycoprotein 1	ORM1	Homo sapiens	917.03	44.78	9	233
P01861	Ig gamma-4 chain C region	IGHG4	Homo sapiens	988.15	50.76	9	231
P10909	Clusterin	CLU	Homo sapiens	867.40	29.18	12	226
P04075	Fructose-bisphosphate aldolase A	ALDOA	Homo sapiens	1022.74	75.55	18	225
P00558	Phosphoglycerate kinase 1	PGK1	Homo sapiens	1016.26	58.03	18	222
P55072	Transitional endoplasmic reticulum ATPase	VCP	Homo sapiens	935.67	46.77	24	221
Q9BXN1	Asporin	ASPN	Homo sapiens	876.49	46.05	13	220
P25705	ATP synthase subunit alpha, mitochondrial	ATP5A1	Homo sapiens	825.16	46.84	19	219
P04004	Vitronectin	VTN	Homo sapiens	976.05	37.24	11	219
Q01062	Spectrin beta chain, non-erythrocytic 1	SPTBN1	Homo sapiens	1081.58	25.34	36	213
P09493	Tropomyosin alpha-1 chain	TPM1	Homo sapiens	776.98	55.99	23	211
P14625	Endoplasmin	HSP90B1	Homo sapiens	887.48	33.00	21	209
P13929	Beta-enolase	ENO3	Homo sapiens	760.72	55.99	16	206
P98160	Basement membrane-specific heparan	HSPG2	Homo sapiens	872.27	17.29	41	202
	sulfate proteoglycan core protein
P60174	Triosephosphate isomerase	TPI1	Homo sapiens	777.86	65.73	13	197
P01877	Ig alpha-2 chain C region	IGHA2	Homo sapiens	1024.76	48.24	9	195
P04792	Heat shock protein beta-1	HSPB1	Homo sapiens	872.68	76.59	12	194
P02144	Myoglobin	MB	Homo sapiens	881.11	65.58	11	192
P16615	Sarcoplasmic/endoplasmic reticulum calcium	ATP2A2	Homo sapiens	728.60	32.63	23	190
	ATPase 2
P07900	Heat shock protein HSP 90-alpha	HSP90AA1	Homo sapiens	744.33	30.05	18	190
P40939	Trifunctional enzyme subunit alpha,	HADHA	Homo sapiens	857.52	40.10	17	187
	mitochondrial
P68104	Elongation factor 1-alpha 1	EEF1A1	Homo sapiens	851.60	40.04	13	186
P07951	Tropomyosin beta chain	TPM2	Homo sapiens	648.46	59.86	22	186
P12035	Keratin, type II cytoskeletal 3	KRT3	Homo sapiens	641.16	16.40	14	184
P30101	Protein disulfide-isomerase A3	PDIA3	Homo sapiens	913.05	47.92	19	183
P10809	60 kDa heat shock protein, mitochondrial	HSPD1	Homo sapiens	881.81	51.66	21	179
Q9BUF5	Tubulin beta-6 chain	TUBB6	Homo sapiens	642.15	48.21	15	178
P23142	Fibulin-1	FBLN1	Homo sapiens	891.96	34.85	17	174
P00338	L-lactate dehydrogenase A chain	LDHA	Homo sapiens	670.19	57.23	17	171
P04264	Keratin, type II cytoskeletal 1	KRT1	Homo sapiens	591.46	24.38	16	171
Q86VB7	Scavenger receptor cysteine-rich type 1	CD163	Homo sapiens	870.30	29.84	20	168
	protein M130
P06753	Tropomyosin alpha-3 chain	TPM3	Homo sapiens	616.79	60.56	25	165
P35749	Myosin-11	MYH11	Homo sapiens	691.99	12.12	16	162
P07237	Protein disulfide-isomerase	P4HB	Homo sapiens	666.06	47.24	19	162
Q99798	Aconitate hydratase, mitochondrial	ACO2	Homo sapiens	794.47	48.97	22	161
P08238	Heat shock protein HSP 90-beta	HSP90AB1	Homo sapiens	587.99	28.31	18	161
P00387	NADH-cytochrome b5 reductase 3	CYB5R3	Homo sapiens	1074.76	57.14	9	161
P40926	Malate dehydrogenase, mitochondrial	MDH2	Homo sapiens	588.03	53.55	14	161
P05976	Myosin light chain 1/3, skeletal muscle	MYL1	Homo sapiens	690.54	79.90	15	157
	isoform
P08133	Annexin A6	ANXA6	Homo sapiens	603.14	36.40	18	156
P62805	Histone H4	HIST1H4A	Homo sapiens	530.41	53.40	8	155
Q06830	Peroxiredoxin-1	PRDX1	Homo sapiens	888.30	60.80	10	155
P19652	Alpha-1-acid glycoprotein 2	ORM2	Homo sapiens	616.30	39.30	6	154
P67936	Tropomyosin alpha-4 chain	TPM4	Homo sapiens	549.57	54.44	17	151
O60437	Periplakin	PPL	Homo sapiens	650.26	22.38	24	151
Q9NZN4	EH domain-containing protein 2	EHD2	Homo sapiens	772.61	40.15	12	150
Q14764	Major vault protein	MVP	Homo sapiens	671.11	36.17	20	150
Q92954	Proteoglycan 4	PRG4	Homo sapiens	569.98	7.91	9	150
P04003	C4b-binding protein alpha chain	C4BPA	Homo sapiens	657.08	43.55	16	149
P00747	Plasminogen	PLG	Homo sapiens	713.50	41.11	18	149
P04220	Ig mu heavy chain disease protein	MUCB	Homo sapiens	773.97	38.11	9	148
O14983	Sarcoplasmic/endoplasmic reticulum calcium	ATP2A1	Homo sapiens	617.91	34.07	25	145
	ATPase 1
P38646	Stress-70 protein, mitochondrial	HSPA9	Homo sapiens	602.67	31.81	15	145
P01871	Ig mu chain C region	IGHM	Homo sapiens	537.31	39.60	13	142
P00352	Retinal dehydrogenase 1	ALDH1A1	Homo sapiens	638.57	46.91	14	141
P27824	Calnexin	CANX	Homo sapiens	653.49	29.73	11	141
P00739	Haptoglobin-related protein	HPR	Homo sapiens	534.40	30.17	13	137
Q16555	Dihydropyrimidinase-related protein 2	DPYSL2	Homo sapiens	566.07	42.48	15	133
P08865	40S ribosomal protein SA	RPSA	Homo sapiens	700.13	45.42	7	133
Q7Z7G0	Target of Nesh-SH3	ABI3BP	Homo sapiens	519.78	22.33	15	132
P32926	Desmoglein-3	DSG3	Homo sapiens	524.96	22.72	15	131
P54652	Heat shock-related 70 kDa protein 2	HSPA2	Homo sapiens	483.73	15.34	9	130
P09525	Annexin A4	ANXA4	Homo sapiens	478.40	40.13	11	128
P02749	Beta-2-glycoprotein 1	APOH	Homo sapiens	514.23	47.25	10	128
P11217	Glycogen phosphorylase, muscle form	PYGM	Homo sapiens	524.04	36.94	22	125
P19823	Inter-alpha-trypsin inhibitor heavy chain H2	ITIH2	Homo sapiens	624.95	22.09	13	125
P13645	Keratin, type I cytoskeletal 10	KRT10	Homo sapiens	411.32	38.36	17	124
P68431	Histone H3.1	HIST1H3A	Homo sapiens	549.86	35.29	3	123
P62937	Peptidyl-prolyl cis-trans isomerase A	PPIA	Homo sapiens	496.09	54.55	7	123
P12236	ADP/ATP translocase 3	SLC25A6	Homo sapiens	396.51	36.58	8	121
P27797	Calreticulin	CALR	Homo sapiens	526.24	53.96	12	121
Q13642	Four and a half LIM domains protein 1	FHL1	Homo sapiens	453.77	36.22	9	121
P12235	ADP/ATP translocase 1	SLC25A4	Homo sapiens	404.10	43.62	11	120
P19105	Myosin regulatory light chain 12A	MYL12A	Homo sapiens	444.26	50.88	7	120
P04114	Apolipoprotein B-100	APOB	Homo sapiens	581.35	8.81	25	120
P0C0L4	Complement C4-A	C4A	Homo sapiens	525.25	21.04	20	119
P13611	Versican core protein	VCAN	Homo sapiens	621.17	6.63	14	119
P07339	Cathepsin D	CTSD	Homo sapiens	425.52	30.83	10	117
P09211	Glutathione S-transferase P	GSTP1	Homo sapiens	559.18	60.48	9	116
Q08043	Alpha-actinin-3	ACTN3	Homo sapiens	412.61	16.98	13	116
Q14624	Inter-alpha-trypsin inhibitor heavy chain H4	ITIH4	Homo sapiens	432.28	26.45	15	116
P08729	Keratin, type II cytoskeletal 7	KRT7	Homo sapiens	350.55	36.46	17	116
P63104	14-3-3 protein zeta/delta	YWHAZ	Homo sapiens	363.16	43.27	8	115
P06744	Glucose-6-phosphate isomerase	GPI	Homo sapiens	514.97	39.61	13	115
P02042	Hemoglobin subunit delta	HBD	Homo sapiens	504.88	57.82	9	114
P07195	L-lactate dehydrogenase B chain	LDHB	Homo sapiens	397.81	30.54	11	113
P37802	Transgelin-2	TAGLN2	Homo sapiens	412.88	44.22	7	112
P0CG05	Ig lambda-2 chain C regions	IGLC2	Homo sapiens	306.17	74.53	5	112
Q07507	Dermatopontin	DPT	Homo sapiens	516.65	60.70	7	111
Q14195	Dihydropyrimidinase-related protein 3	DPYSL3	Homo sapiens	510.80	42.63	14	110
P04844	Dolichyl-diphosphooligosaccharide--protein	RPN2	Homo sapiens	515.84	35.18	12	108
	glycosyltransferase subunit 2
Q13835	Plakophilin-1	PKP1	Homo sapiens	419.65	26.64	14	107
P04843	Dolichyl-diphosphooligosaccharide--protein	RPN1	Homo sapiens	511.79	35.26	14	107
	glycosyltransferase subunit 1
P06702	Protein S100-A9	S100A9	Homo sapiens	462.23	74.56	7	107
P08572	Collagen alpha-2(IV) chain	COL4A2	Homo sapiens	148.22	7.48	7	105
P61978	Heterogeneous nuclear ribonucleoprotein K	HNRNPK	Homo sapiens	395.65	25.27	8	101
P05091	Aldehyde dehydrogenase, mitochondrial	ALDH2	Homo sapiens	303.86	31.14	12	100
P00367	Glutamate dehydrogenase 1, mitochondrial	GLUD1	Homo sapiens	394.06	35.48	14	100
P30086	Phosphatidylethanolamine-binding protein 1	PEBP1	Homo sapiens	565.01	70.59	8	100
P01008	Antithrombin-III	SERPINC1	Homo sapiens	418.47	32.54	12	99
P36578	60S ribosomal protein L4	RPL4	Homo sapiens	379.47	32.55	11	99
P24844	Myosin regulatory light polypeptide 9	MYL9	Homo sapiens	404.88	56.40	7	98
P02774	Vitamin D-binding protein	GC	Homo sapiens	425.10	29.32	10	98
P17661	Desmin	DES	Homo sapiens	352.16	41.91	19	97
P30048	Thioredoxin-dependent peroxide reductase,	PRDX3	Homo sapiens	533.07	48.05	7	97
	mitochondrial
P31949	Protein S100-A11	S100A11	Homo sapiens	439.33	71.43	6	97
P23528	Cofilin-1	CFL1	Homo sapiens	418.44	63.25	9	96
P04217	Alpha-1B-glycoprotein	A1BG	Homo sapiens	398.41	38.79	11	96
P35221	Catenin alpha-1	CTNNA1	Homo sapiens	449.17	42.49	20	95
Q12805	EGF-containing fibulin-like extracellular	EFEMP1	Homo sapiens	462.35	39.35	12	95
	matrix protein 1
P05141	ADP/ATP translocase 2	SLC25A5	Homo sapiens	308.16	27.52	7	95
P17174	Aspartate aminotransferase, cytoplasmic	GOT1	Homo sapiens	386.92	52.78	16	94
P63244	Guanine nucleotide-binding protein subunit	GNB2L1	Homo sapiens	428.30	52.37	10	94
	beta-2-like 1
P05783	Keratin, type I cytoskeletal 18	KRT18	Homo sapiens	313.43	44.42	13	92
P17540	Creatine kinase S-type, mitochondrial	CKMT2	Homo sapiens	380.25	49.16	14	92
P60660	Myosin light polypeptide 6	MYL6	Homo sapiens	335.10	52.98	8	92
P22352	Glutathione peroxidase 3	GPX3	Homo sapiens	296.23	29.20	5	92
P62258	14-3-3 protein epsilon	YWHAE	Homo sapiens	273.86	33.33	8	90
P00751	Complement factor B	CFB	Homo sapiens	335.25	28.01	14	90
P12532	Creatine kinase U-type, mitochondrial	CKMT1A	Homo sapiens	451.15	37.17	9	90
Q14315	Filamin-C	FLNC	Homo sapiens	343.94	13.72	23	90
P39656	Dolichyl-diphosphooligosaccharide--protein	DDOST	Homo sapiens	642.76	25.22	6	90
	glycosyltransferase 48 kDa subunit
P40925	Malate dehydrogenase, cytoplasmic	MDH1	Homo sapiens	339.35	36.23	9	89
Q00872	Myosin-binding protein C, slow-type	MYBPC1	Homo sapiens	320.23	26.29	22	89
P22695	Cytochrome b-c1 complex subunit 2,	UQCRC2	Homo sapiens	372.95	36.20	12	89
	mitochondrial
Q7Z406	Myosin-14	MYH14	Homo sapiens	346.95	5.26	7	89
Q96A32	Myosin regulatory light chain 2, skeletal	MYLPF	Homo sapiens	301.63	69.82	11	88
	muscle isoform
P05388	60S acidic ribosomal protein P0	RPLP0	Homo sapiens	281.69	39.43	8	88
Q05639	Elongation factor 1-alpha 2	EEF1A2	Homo sapiens	402.16	30.67	9	87
P98095	Fibulin-2	FBLN2	Homo sapiens	355.37	22.97	16	87
P00568	Adenylate kinase isoenzyme 1	AK1	Homo sapiens	457.63	46.91	7	86
P61626	Lysozyme C	LYZ	Homo sapiens	396.57	46.62	5	86
P18669	Phosphoglycerate mutase 1	PGAM1	Homo sapiens	367.15	59.84	11	86
P62917	60S ribosomal protein L8	RPL8	Homo sapiens	397.44	36.96	6	86
Q14204	Cytoplasmic dynein 1 heavy chain 1	DYNC1H1	Homo sapiens	405.37	7.25	19	85
P29401	Transketolase	TKT	Homo sapiens	482.56	31.94	9	85
P02750	Leucine rich alpha-2-glycoprotein	LRG1	Homo sapiens	377.99	41.50	9	84
P21980	Protein-glutamine gamma-	TGM2	Homo sapiens	357.31	30.57	13	84
	glutamyltransferase 2
P18206	Vinculin	VCL	Homo sapiens	289.28	23.46	17	84
P61981	14-3-3 protein gamma	YWHAG	Homo sapiens	329.88	42.51	7	83
P21964	Catechol O-methyltransferase	COMT	Homo sapiens	392.61	52.40	9	83
P62424	60S ribosomal protein L7a	RPL7A	Homo sapiens	299.36	35.34	10	83
P0DJI8	Serum amyloid A-1 protein	SAA1	Homo sapiens	370.93	66.39	7	83
P05023	Sodium/potassium-transporting ATPase	ATP1A1	Homo sapiens	290.15	17.69	13	81
	subunit alpha-1
P62158	Calmodulin	CALM1	Homo sapiens	307.86	46.98	7	81
P55084	Trifunctional enzyme subunit beta,	HADHB	Homo sapiens	360.39	39.03	11	81
	mitochondrial
Q14697	Neutral alpha-glucosidase AB	GANAB	Homo sapiens	415.60	25.00	12	81
P62879	Guanine nucleotide-binding protein	GNB2	Homo sapiens	308.38	52.35	11	81
	G(I)/G(S)/G(T) subunit beta-2
P07858	Cathepsin B	CTSB	Homo sapiens	379.84	27.73	7	80
P06748	Nucleophosmin	NPM1	Homo sapiens	408.27	24.15	5	80
P05155	Plasma protease C1 inhibitor	SERPING1	Homo sapiens	295.14	22.40	10	79
Q15084	Protein disulfide-isomerase A6	PDIA6	Homo sapiens	302.05	33.41	9	79
P24821	Tenascin	TNC	Homo sapiens	328.11	16.13	19	79
P31947	14-3-3 protein sigma	SFN	Homo sapiens	358.29	55.65	10	78
Q03135	Caveolin-1	CAV1	Homo sapiens	368.92	46.63	6	78
P35222	Catenin beta-1	CTNNB1	Homo sapiens	428.51	16.77	8	78
P26038	Moesin	MSN	Homo sapiens	287.39	24.09	11	78
P22897	Macrophage mannose receptor 1	MRC1	Homo sapiens	357.42	15.87	14	78
Q15365	Poly(rC)-binding protein 1	PCBP1	Homo sapiens	349.30	32.02	7	78
P11216	Glycogen phosphorylase, brain form	PYGB	Homo sapiens	311.63	22.18	13	78
P21796	Voltage-dependent anion-selective channel	VDAC1	Homo sapiens	295.47	51.24	9	77
	protein 1
P27348	14-3-3 protein theta	YWHAQ	Homo sapiens	238.77	38.78	8	76
P45378	Troponin T, fast skeletal muscle	TNNT3	Homo sapiens	371.79	36.80	11	76
Q92928	Putative Ras-related protein Rab-1C	RAB1C	Homo sapiens	204.29	31.34	5	76
P52895	Aldo-keto reductase family 1 member C2	AKR1C2	Homo sapiens	370.32	36.53	7	75
P02792	Ferritin light chain	FTL	Homo sapiens	374.53	44.57	6	74
P27105	Erythrocyte band 7 integral membrane	STOM	Homo sapiens	355.48	39.24	6	74
	protein
P61204	ADP-ribosylation factor 3	ARF3	Homo sapiens	264.46	54.70	8	73
P63241	Eukaryotic translation initiation factor 5A-1	EIF5A	Homo sapiens	402.91	61.69	7	73
Q8TDL5	BPI fold-containing family B member 1	BPIFB1	Homo sapiens	280.43	39.88	13	73
P35232	Prohibitin	PHB	Homo sapiens	392.68	47.06	7	73
P00505	Aspartate aminotransferase, mitochondrial	GOT2	Homo sapiens	322.70	40.00	11	73
P17931	Galectin-3	LGALS3	Homo sapiens	276.89	28.40	6	73
O95994	Anterior gradient protein 2 homolog	AGR2	Homo sapiens	266.91	54.86	6	72
P26641	Elongation factor 1-gamma	EEF1G	Homo sapiens	241.88	26.32	8	71
P23396	40S ribosomal protein S3	RPS3	Homo sapiens	262.77	50.62	10	71
P45880	Voltage-dependent anion-selective channel	VDAC2	Homo sapiens	309.07	38.78	8	71
	protein 2
Q08211	ATP-dependent RNA helicase A	DHX9	Homo sapiens	350.79	11.97	9	70
P05387	60S acidic ribosomal protein P2	RPLP2	Homo sapiens	308.01	85.22	6	70
P31946	14-3-3 protein beta/alpha	YWHAB	Homo sapiens	223.98	34.55	7	69
P09972	Fructose-bisphosphate aldolase C	ALDOC	Homo sapiens	307.69	20.33	5	69
P30740	Leukocyte elastase inhibitor	SERPINB1	Homo sapiens	297.65	34.83	8	69
P39023	60S ribosomal protein L3	RPL3	Homo sapiens	308.88	26.30	8	69
P62081	40S ribosomal protein S7	RPS7	Homo sapiens	329.43	37.11	4	69
Q01469	Fatty acid-binding protein, epidermal	FABP5	Homo sapiens	304.75	41.48	5	68
P15259	Phosphoglycerate mutase 2	PGAM2	Homo sapiens	295.35	56.92	12	68
P30041	Peroxiredoxin-6	PRDX6	Homo sapiens	299.12	37.05	6	68
P52209	6-phosphogluconate dehydrogenase, decarboxylating	PGD	Homo sapiens	348.83	19.25	6	67
P31040	Succinate dehydrogenase [ubiquinone]	SDHA	Homo sapiens	321.54	20.93	9	67
	flavoprotein subunit, mitochondrial
P07099	Epoxide hydrolase 1	EPHX1	Homo sapiens	330.10	32.75	7	67
P22626	Heterogeneous nuclear ribonucleoproteins	HNRNPA2B1	Homo sapiens	239.94	25.78	9	67
	A2/B1
P62701	40S ribosomal protein S4, X isoform	RPS4X	Homo sapiens	227.75	41.44	10	67
P07384	Calipain-1 catalytic subunit	CAPN1	Homo sapiens	258.35	26.47	11	66
P99999	Cytochrome c	CYCS	Homo sapiens	241.24	48.57	5	66
Q16777	Histone H2A type 2-C	HIST2H2AC	Homo sapiens	184.31	63.57	6	66
P52272	Heterogeneous nuclear ribonucleoprotein M	HNRNPM	Homo sapiens	236.69	21.51	12	66
P60842	Eukaryotic initiation factor 4A-I	EIF4A1	Homo sapiens	210.21	41.38	12	66
P10916	Myosin regulatory light chain 2,	MYL2	Homo sapiens	242.44	77.71	10	66
	ventricular/cardiac muscle isoform
Q6NZI2	Polymerase I and transcript release factor	PTRF	Homo sapiens	253.00	22.56	7	66
P29508	Serpin B3	SERPINB3	Homo sapiens	230.90	37.44	11	66
Q9Y490	Talin-1	TLN1	Homo sapiens	245.23	11.26	15	66
P39060	Collagen alpha-1(XVIII) chain	COL18A1	Homo sapiens	311.69	7.58	6	66
Q13361	Microfibrillar-associated protein 5	MFAP5	Homo sapiens	229.94	18.50	3	65
P32119	Peroxiredoxin-2	PRDX2	Homo sapiens	302.38	32.32	6	65
P01042	Kininogen-1	KNG1	Homo sapiens	254.20	21.89	9	64
P13489	Ribonuclease inhibitor	RNH1	Homo sapiens	294.50	31.67	8	64
Q9HCY8	Protein S100-A14	S100A14	Homo sapiens	240.31	65.38	5	64
P04179	Superoxide dismutase [Mn], mitochondrial	SOD2	Homo sapiens	285.33	61.71	7	64
O75390	Citrate synthase, mitochondrial	CS	Homo sapiens	348.67	30.26	8	63
P08294	Extracellular superoxide dismutase [Cu—Zn]	SOD3	Homo sapiens	255.58	26.67	5	63
Q15661	Tryptase alpha/beta-1	TPSAB1	Homo sapiens	232.08	22.18	6	63
Q9Y277	Voltage-dependent anion-selective channel	VDAC3	Homo sapiens	225.78	27.21	5	63
	protein 3
P00403	Cytochrome c oxidase subunit 2	MT-CO2	Homo sapiens	210.74	20.26	3	62
P49411	Elongation factor Tu, mitochondrial	TUFM	Homo sapiens	266.69	25.00	8	62
P26599	Polypyrimidine tract-binding protein 1	PTBP1	Homo sapiens	332.59	32.20	9	62
P09382	Galectin-1	LGALS1	Homo sapiens	210.45	65.93	6	61
P00734	Prothrombin	F2	Homo sapiens	251.04	30.23	12	61
P31415	Calsequestrin-1	CASQ1	Homo sapiens	318.32	29.29	7	61
P25311	Zinc-alpha-2-glycoprotein	AZGP1	Homo sapiens	254.10	29.53	6	61
P48735	Isocitrate dehydrogenase [NADP],	IDH2	Homo sapiens	207.55	27.65	11	60
	mitochondrial
P09622	Dihydrolipoyl dehydrogenase, mitochondrial	DLD	Homo sapiens	333.83	20.24	6	60
Q6DD88	Atlastin-3	ATL3	Homo sapiens	348.75	32.72	8	59
P13010	X-ray repair cross-complementing protein 5	XRCC5	Homo sapiens	310.59	25.55	10	59
P22392	Nucleoside diphosphate kinase B	NME2	Homo sapiens	226.78	35.53	4	59
P27635	60S ribosomal protein L10	RPL10	Homo sapiens	253.21	36.92	8	59
P30153	Serine/threonine-protein phosphatase 2A 65	PPP2R1A	Homo sapiens	245.66	14.09	5	58
	kDa regulatory subunit A alpha isoform
P18085	ADP-ribosylation factor 4	ARF4	Homo sapiens	200.39	48.33	7	58
O43852	Calumenin	CALU	Homo sapiens	344.28	36.19	6	58
Q99714	3-hydroxyacyl-CoA dehydrogenase type-2	HSD17B10	Homo sapiens	256.09	44.83	7	58
P47929	Galectin-7	LGALS7	Homo sapiens	267.04	55.15	5	58
P50914	60S ribosomal protein L14	RPL14	Homo sapiens	164.83	24.19	5	58
P04080	Cystatin-B	CSTB	Homo sapiens	255.39	45.92	3	57
P12277	Creatine kinase B-type	CKB	Homo sapiens	243.55	38.85	9	57
P08590	Myosin light chain 3	MYL3	Homo sapiens	180.14	47.69	8	57
P15880	40S ribosomal protein S2	RPS2	Homo sapiens	247.88	25.94	5	57
P50454	Serpin H1	SERPINH1	Homo sapiens	255.28	42.11	12	57
Q96TA1	Niban-like protein 1	FAM129B	Homo sapiens	273.85	13.40	6	57
P48047	ATP synthase subunit O, mitochondrial	ATP5O	Homo sapiens	198.34	44.60	6	56
P07954	Fumarate hydratase, mitochondrial	FH	Homo sapiens	315.14	24.12	5	56
Q99623	Prohibitin-2	PHB2	Homo sapiens	201.31	37.12	8	56
P02743	Serum amyloid P-component	APCS	Homo sapiens	175.11	25.11	6	56
P20929	Nebulin	NEB	Homo sapiens	181.95	5.88	21	56
P62979	Ubiquitin-40S ribosomal protein S27a	RPS27A	Homo sapiens	194.01	28.21	3	56
P07910	Heterogeneous nuclear ribonucleoproteins	HNRNPC	Homo sapiens	178.69	20.92	7	55
	C1/C2
P50995	Annexin A11	ANXA11	Homo sapiens	193.16	21.98	9	54
P02649	Apolipoprotein E	APOE	Homo sapiens	205.73	31.55	7	54
P04632	Calpain small subunit 1	CAPNS1	Homo sapiens	267.00	30.97	4	54
O75367	Core histone macro-H2A.1	H2AFY	Homo sapiens	224.12	29.03	7	54
Q32P51	Heterogeneous nuclear ribonucleoprotein	HNRNPA1L2	Homo sapiens	198.73	23.13	7	54
	A1-like 2
P16403	Histone H1.2	HIST1H1C	Homo sapiens	158.83	20.19	7	53
Q9NZM1	Myoferlin	MYOF	Homo sapiens	225.43	8.98	11	53
P05109	Protein S100-A8	S100A8	Homo sapiens	162.26	40.86	4	53
Q14134	Tripartite motif-containing protein 29	TRIM29	Homo sapiens	210.80	21.43	9	53
P35443	Thrombospondin-4	THBS4	Homo sapiens	243.36	25.39	13	53
Q05707	Collagen alpha-1(XIV) chain	COL14A1	Homo sapiens	231.55	8.35	11	52
Q99497	Protein DJ-1	PARK7	Homo sapiens	236.62	56.61	6	52
Q13838	Spliceosome RNA helicase DDX39B	DDX39B	Homo sapiens	210.91	18.93	5	52
P35527	Keratin type I cytoskeletal 9	KRT9	Homo sapiens	258.81	18.94	7	51
O94973	AP-2 complex subunit alpha-2	AP2A2	Homo sapiens	229.57	14.91	7	51
Q14574	Desmocollin-3	DSC3	Homo sapiens	284.04	16.52	8	51
P04889	Guanine nucleotide-binding protein G(i)	GNAI2	Homo sapiens	181.43	32.39	8	51
	subunit alpha-2
P0C0S5	Histone H2A.Z	H2AFZ	Homo sapiens	157.41	42.97	4	51
P46777	60S ribosomal protein L5	RPL5	Homo sapiens	211.39	23.57	5	51
P05386	60S acidic ribosomal protein P1	RPLP1	Homo sapiens	201.61	66.67	3	51
O75083	WD repeat-containing protein 1	WDR1	Homo sapiens	312.47	33.00	9	51
Q02218	2-oxoglutarate dehydrogenase, mitochondrial	OGDH	Homo sapiens	284.56	18.18	9	51
P60033	CD81 antigen	CD81	Homo sapiens	232.36	25.00	3	50
P09429	High mobility group protein B1	HMGB1	Homo sapiens	221.28	27.91	5	50
P30838	Aldehyde dehydrogenase, dimeric NADP-	ALDH3A1	Homo sapiens	188.52	29.36	7	50
	preferring
P16152	Carbonyl reductase [NADPH] 1	CBR1	Homo sapiens	205.13	53.07	11	49
P46782	40S ribosomal protein S5	RPS5	Homo sapiens	160.59	33.33	6	49
P01880	Ig delta chain C region	IGHD	Homo sapiens	265.21	32.29	7	49
P23284	Peptidyl-prolyl cis-trans isomerase B	PPIB	Homo sapiens	154.36	43.52	9	49
P61158	Actin-related protein 3	ACTR3	Homo sapiens	229.45	20.10	4	48
P01620	Ig kappa chain V-III region SIE	KV302	Homo sapiens	210.50	39.45	3	48
Q9H8H3	Methyltransferase-like protein 7A	METTL7A	Homo sapiens	172.04	31.56	5	48
Q9NQC3	Reticulon-4	RTN4	Homo sapiens	230.34	7.30	5	48
Q9Y3Z3	SAM domain and HD domain-containing	SAMHD1	Homo sapiens	215.44	23.32	9	48
	protein 1
P54296	Myomesin-2	MYOM2	Homo sapiens	209.59	18.09	13	48
P21926	CD9 antigen	CD9	Homo sapiens	267.46	15.35	2	47
P43490	Nicotinamide phosphoribosyltransferase	NAMPT	Homo sapiens	265.35	33.20	9	47
P48788	Troponin I, fast skeletal muscle	TNNI2	Homo sapiens	247.76	42.86	8	47
P17655	Calpain-2 catalytic subunit	CAPN2	Homo sapiens	228.83	20.57	7	47
Q00839	Heterogeneous nuclear ribonucleoprotein U	HNRNPU	Homo sapiens	201.33	12.36	5	47
Q02878	60S ribosomal protein L6	RPL6	Homo sapiens	164.14	26.39	7	46
P25398	40S ribosomal protein S12	RPS12	Homo sapiens	204.42	47.73	5	46
A6NMZ7	Collagen alpha-6(VI) chain	COL6A6	Homo sapiens	223.43	9.72	13	46
Q92817	Envoplakin	EVPL	Homo sapiens	238.79	11.95	13	46
P62263	40S ribosomal protein S14	RPS14	Homo sapiens	191.81	63.58	5	45
P62241	40S ribosomal protein S8	RPS8	Homo sapiens	158.52	34.62	6	45
P43304	Glycerol-3-phosphate dehydrogenase,	GPD2	Homo sapiens	237.37	13.76	5	45
	mitochondrial
P15121	Aldose reductase	AKR1B1	Homo sapiens	205.46	27.22	5	44
P02747	Complement C1q subcomponent subunit C	C1QC	Homo sapiens	149.99	25.31	5	44
P02748	Complement component C9	C9	Homo sapiens	175.08	13.60	6	44
P24534	Elongation factor 1-beta	EEF1B2	Homo sapiens	185.03	34.67	4	44
Q07020	60S ribosomal protein L18	RPL18	Homo sapiens	144.36	25.00	4	44
P61247	40S ribosomal protein S3a	RPS3A	Homo sapiens	176.21	21.59	4	44
P00441	Superoxide dismutase [Cu—Zn]	SOD1	Homo sapiens	360.54	53.90	3	44
Q01995	Transgelin	TAGLN	Homo sapiens	133.46	31.34	5	44
O75369	Filamin-B	FLNB	Homo sapiens	153.21	4.96	8	44
P26022	Pentraxin-related protein PTX3	PTX3	Homo sapiens	169.22	22.57	7	44
P09758	Tumor-associated calcium signal transducer 2	TACSTD2	Homo sapiens	189.68	32.82	7	44
Q9Y2Q3	Glutathione S-transferase kappa 1	GSTK1	Homo sapiens	190.62	32.74	5	43
Q00325	Phosphate carrier protein, mitochondrial	SLC25A3	Homo sapiens	152.13	19.06	5	43
Q06323	Proteasome activator complex subunit 1	PSME1	Homo sapiens	162.04	23.29	5	43
Q16647	Prostacyclin synthase	PTGIS	Homo sapiens	161.75	33.60	11	43
P29034	Protein S100-A2	S100A2	Homo sapiens	137.60	27.55	4	43
Q9Y6N5	Sulfide-quinone oxidoreductase,	SQRDL	Homo sapiens	157.67	23.78	8	43
	mitochondrial
P78371	T-complex protein 1 subunit beta	CCT2	Homo sapiens	204.85	25.05	8	43
P22314	Ubiquitin-like modifier-activating enzyme 1	UBA1	Homo sapiens	159.50	12.00	8	43
Q04917	14-3-3 protein eta	YWHAH	Homo sapiens	123.80	18.29	4	42
P05997	Collagen alpha-2(V) chain	COL5A2	Homo sapiens	51.56	4.47	4	42
P00390	Glutathione reductase, mitochondrial	GSR	Homo sapiens	187.41	23.18	6	42
P52597	Heterogeneous nuclear ribonucleoprotein F	HNRNPF	Homo sapiens	194.08	21.69	5	42
P18428	Lipopolysaccharide-binding protein	LBP	Homo sapiens	191.95	14.76	5	42
P12273	Prolactin-inducible protein	PIP	Homo sapiens	165.44	39.04	4	42
P07737	Profilin-1	PFN1	Homo sapiens	121.34	53.57	6	42
Q15286	Ras-related protein Rab-35	RAB35	Homo sapiens	116.52	19.90	3	42
Q07960	Rho GTPase-activating protein 1	ARHGAP1	Homo sapiens	185.83	24.60	5	42
P62888	60S ribosomal protein L30	RPL30	Homo sapiens	156.93	51.30	4	42
P31930	Cytochrome b-c1 complex subunit 1,	UQCRC1	Homo sapiens	194.33	30.00	8	42
	mitochondrial
P02746	Complement C1q subcomponent subunit B	C1QB	Homo sapiens	151.74	20.55	4	42
P49748	Very long-chain specific acyl-CoA	ACADVL	Homo sapiens	188.16	17.10	7	41
	dehydrogenase, mitochondrial
P56134	ATP synthase subunit f, mitochondrial	ATP5J2	Homo sapiens	128.69	25.53	2	41
O14880	Microsomal glutathione S-transferase 3	MGST3	Homo sapiens	209.57	33.55	3	41
P61106	Ras-related protein Rab-14	RAB14	Homo sapiens	139.72	33.02	4	41
P32969	60S ribosomal protein L9	RPL9	Homo sapiens	206.78	50.52	7	41
Q01518	Adenylyl cyclase-associated protein 1	CAP1	Homo sapiens	116.36	18.11	6	41
P0DJI9	Serum amyloid A-2 protein	SAA2	Homo sapiens	152.96	47.54	5	41
P02760	Protein AMBP	AMBP	Homo sapiens	130.27	20.17	5	40
O00571	ATP-dependent RNA helicase DDX3X	DDX3X	Homo sapiens	136.76	14.80	7	40
P04196	Histidine-rich glycoprotein	HRG	Homo sapiens	146.71	23.24	8	40
P19338	Nucleolin	NCL	Homo sapiens	118.67	17.89	8	40
P62826	GTP-binding nuclear protein Ran	RAN	Homo sapiens	140.17	29.63	5	40
P30050	60S ribosomal protein L12	RPL12	Homo sapiens	142.78	54.55	6	40
Q16851	UTP--glucose-1-phosphate	UGP2	Homo sapiens	180.79	24.02	8	40
	uridylyltransferase
P30044	Peroxiredoxin-5, mitochondrial	PRDX5	Homo sapiens	134.47	39.25	6	40
P15144	Aminopeptidase N	ANPEP	Homo sapiens	199.99	10.44	5	39
Q9Y281	Cofilin-2	CFL2	Homo sapiens	145.82	43.98	5	39
P14854	Cytochrome c oxidase subunit 6B1	COX6B1	Homo sapiens	138.41	56.98	4	39
Q14974	Importin subunit beta-1	KPNB1	Homo sapiens	167.13	10.96	6	39
P18621	60S ribosomal protein L17	RPL17	Homo sapiens	170.36	37.50	6	39
P62829	60S ribosomal protein L23	RPL23	Homo sapiens	159.78	25.00	2	39
P46783	40S ribosomal protein S10	RPS10	Homo sapiens	125.03	23.64	3	39
Q15019	Septin 2	41884	Homo sapiens	201.93	22.44	5	39
Q9BTV4	Transmembrane protein 43	TMEM43	Homo sapiens	256.33	26.25	5	39
P02585	Troponin C, skeletal muscle	TNNC2	Homo sapiens	160.86	56.25	6	39
P08574	Cytochrome c1, heme protein, mitochondrial	CYC1	Homo sapiens	176.16	20.00	4	39
P36957	Dihydrolipoyllysine-residue	DLST	Homo sapiens	118.88	16.56	6	39
	succinyltransferase component of 2-
	oxoglutarate dehydrogenase complex, mitochondrial
P08697	Alpha-2-antiplasmin	SERPINF2	Homo sapiens	212.46	16.50	4	38
P31943	Heterogeneous nuclear ribonucleoprotein H	HNRNPH1	Homo sapiens	143.80	16.26	5	38
P19827	Inter-alpha-trypsin inhibitor heavy chain H1	ITIH1	Homo sapiens	172.16	9.22	5	38
P12956	X-ray repair cross-complementing protein 6	XRCC6	Homo sapiens	128.28	18.06	8	38
P01617	Ig kappa chain V-II region TEW	KV204	Homo sapiens	147.26	32.74	2	38
P20700	Lamin-B1	LMNB1	Homo sapiens	115.63	22.01	10	38
P53621	Coatomer subunit alpha	COPA	Homo sapiens	177.86	10.05	6	38
P20674	Cytochrome c oxidase subunit 5A, mitochondrial	COX5A	Homo sapiens	152.37	48.00	6	38
Q16891	Mitochondrial inner membrane protein	IMMT	Homo sapiens	196.93	13.98	6	37
P62906	60S ribosomal protein L10a	RPL10A	Homo sapiens	151.26	31.80	6	37
P35268	60S ribosomal protein L22	RPL22	Homo sapiens	208.22	39.06	3	37
P46781	40S ribosomal protein S9	RPS9	Homo sapiens	93.51	29.38	6	37
P49368	T-complex protein 1 subunit gamma	CCT3	Homo sapiens	139.77	24.95	9	37
P13805	Troponin T, slow skeletal muscle	TNNT1	Homo sapiens	178.15	26.62	6	37
O14773	Tripeptidyl-peptidase 1	TPP1	Homo sapiens	175.66	19.72	5	37
P15088	Mast cell carboxypeptidase A	CPA3	Homo sapiens	100.59	16.31	6	37
P21397	Amine oxidase [flavin-containing] A	MAOA	Homo sapiens	210.61	23.34	7	36
O00264	Membrane-associated progesterone receptor	PGRMC1	Homo sapiens	230.02	26.15	2	36
	component 1
P62841	40S ribosomal protein S15	RPS15	Homo sapiens	217.34	60.69	5	36
P62847	40S ribosomal protein S24	RPS24	Homo sapiens	114.91	28.57	3	36
Q99832	T-complex protein 1 subunit eta	CCT7	Homo sapiens	174.83	16.21	5	36
P02766	Transthyretin	TTR	Homo sapiens	198.08	58.50	5	36
P50395	Rab GDP dissociation inhibitor beta	GDI2	Homo sapiens	184.88	29.21	7	35
Q15366	Poly(rC)-binding protein 2	PCBP2	Homo sapiens	124.46	28.77	6	35
P36671	Phosphoglucomutase-1	PGM1	Homo sapiens	117.76	26.51	9	35
P60953	Cell division control protein 42 homolog	CDC42	Homo sapiens	132.02	40.31	5	35
Q03252	Lamin-B2	LMNB2	Homo sapiens	99.19	16.67	8	34
O00151	PDZ and LIM domain protein 1	PDLIM1	Homo sapiens	219.59	20.97	3	34
P61313	60S ribosomal protein L15	RPL15	Homo sapiens	116.87	24.02	4	34
P63010	AP-2 complex subunit beta	AP2B1	Homo sapiens	144.72	8.96	4	33
P52907	F-actin-capping protein subunit alpha-1	CAPZA1	Homo sapiens	118.74	22.38	4	33
O00299	Chloride intracellular channel protein 1	CLIC1	Homo sapiens	100.54	24.90	5	33
F59665	Neutrophil defensin 1	DEFA1	Homo sapiens	99.41	20.21	3	33
P30084	Enoyl-CoA hydratase, mitochondrial	ECHS1	Homo sapiens	110.92	16.21	3	33
P02794	Ferritin heavy chain	FTH1	Homo sapiens	190.73	38.80	4	33
P31150	Rab GDP dissociation inhibitor alpha	GDI1	Homo sapiens	177.37	19.91	4	33
O43390	Heterogeneous nuclear ribonucleoprotein R	HNRNPR	Homo sapiens	123.66	11.22	6	33
P28331	NADH-ubiquinone oxidoreductase 75 kDa	NDUFS1	Homo sapiens	146.80	14.86	6	33
	subunit, mitochondrial
P62140	Serine/threonine-protein phosphatase PP1-	PPP1CB	Homo sapiens	144.77	25.69	5	33
	beta catalytic subunit
P08134	Rho-related GTP-binding protein RhoC	RHOC	Homo sapiens	113.02	36.27	4	33
Q96FQ6	Protein S100-A16	S100A16	Homo sapiens	111.92	63.11	5	33
Q10567	AP-1 complex subunit beta-1	AP1B1	Homo sapiens	155.87	4.95	3	33
P51648	Fatty aldehyde dehydrogenase	ALDH3A2	Homo sapiens	101.08	11.34	4	32
P61769	Beta-2-microglobulin	B2M	Homo sapiens	129.55	35.29	3	32
P30042	ES1 protein homolog, mitochondrial	C21orf33	Homo sapiens	184.38	26.87	3	32
Q14103	Heterogeneous nuclear ribonucleoprotein D0	HNRNPD	Homo sapiens	96.00	19.72	5	32
P46778	60S ribsomal protein L21	RPL21	Homo sapiens	112.78	31.87	4	32
P62910	60S ribsomal protein L32	RPL32	Homo sapiens	102.48	27.41	3	32
P18124	60S ribsomal protein L7	RPL7	Homo sapiens	123.75	34.27	7	32
P51991	Heterogeneous nuclear ribonucleoprotein A3	HNRNPA3	Homo sapiens	118.23	18.52	6	32
P62249	40S ribosomal protein S16	RPS16	Homo sapiens	92.94	30.14	4	32
P62269	40S ribosomal protein S18	RPS18	Homo sapiens	82.25	36.84	6	32
Q8NBS9	Thioredoxin domain-containing protein 5	TXNDC5	Homo sapiens	190.89	16.90	5	32
P55268	Laminin subunit beta-2	LAMB2	Homo sapiens	137.11	7.79	7	32
P07108	Acyl-CoA-binding protein	DBI	Homo sapiens	158.66	41.38	2	31
Q96KP4	Cytosolic non-specific dipeptidase	CNDP2	Homo sapiens	124.80	17.05	5	31
P02511	Alpha-crystallin B chain	CRYAB	Homo sapiens	115.45	34.29	5	31
P49773	Histidine triad nucleotide-binding protein 1	HINT1	Homo sapiens	238.93	54.76	3	31
Q15181	Inorganic pyrophosphatase	PPA1	Homo sapiens	106.49	28.72	5	31
P83731	60S ribosomal protein L24	RPL24	Homo sapiens	103.39	19.11	3	31
Q53EL6	Programmed cell death protein 4	PDCD4	Homo sapiens	151.18	11.73	3	31
P29692	Elongation factor 1-delta	EEF1D	Homo sapiens	121.20	21.71	4	30
P01625	Ig kappa chain V-IV region Len	KV402	Homo sapiens	123.28	23.68	2	30
P55058	Phospholipid transfer protein	PLTP	Homo sapiens	118.29	16.63	5	30
P51148	Ras-related protein Rab-5C	RAB5C	Homo sapiens	88.46	18.06	3	30
Q02543	60S ribsomal protein L18a	RPL18A	Homo sapiens	82.92	25.00	4	30
P0CW22	40S ribsomal protein S17-like	RPS17L	Homo sapiens	84.85	49.63	4	30
P39019	40S ribsomal protein S19	RPS19	Homo sapiens	93.93	43.45	7	30
P24752	Acetyl-CoA acetyltransferase, mitochondrial	ACAT1	Homo sapiens	102.46	16.63	5	30
P36955	Pigment epithelium-derived factor	SERPINF1	Homo sapiens	123.08	27.03	6	30
P28838	Cytosol aminopeptidase	LAP3	Homo sapiens	128.37	16.18	5	29
P00395	Cytochrome c oxidase subunit 1	MT-CO1	Homo sapiens	114.84	7.60	2	29
P10915	Hyaluronan and proteoglycan link protein 1	HAPLN1	Homo sapiens	128.51	36.16	7	29
O75112	LIM domain-binding protein 3	LDB3	Homo sapiens	159.55	12.38	6	29
Q14210	Lymphocyte antigen 6D	LY6D	Homo sapiens	85.31	16.41	2	29
Q13162	Peroxiredoxin-4	PRDX4	Homo sapiens	110.59	26.57	5	29
P51149	Ras-related protein Rab-7a	RAB7A	Homo sapiens	91.57	46.38	7	29
Q13228	Selenium-binding protein 1	SELENBP1	Homo sapiens	140.38	25.21	7	29
P23246	Splicing factor, proline- and glutamine-rich	SFPQ	Homo sapiens	146.88	6.79	3	29
P03973	Antileukoproteinase	SLPI	Homo sapiens	105.65	20.45	2	29
P49755	Transmembrane emp24 domain-containing	TMED10	Homo sapiens	95.34	18.26	4	29
	protein 10
P07919	Cytochrome b-c1 complex subunit 6,	UQCRH	Homo sapiens	151.77	29.67	2	29
	mitochondrial
P00325	Alcohol dehydrogenase 1B	ADH1B	Homo sapiens	143.26	15.73	5	28
P07451	Carbonic anhydrase 3	CA3	Homo sapiens	103.24	45.77	7	28
P08311	Cathepsin G	CTSG	Homo sapiens	86.82	18.43	4	28
P23946	Chymase	CMA1	Homo sapiens	104.09	38.87	7	28
P15311	Ezrin	EZR	Homo sapiens	94.52	10.41	5	28
P08237	6-phosphofructokinase, muscle type	PFKM	Homo sapiens	103.32	22.82	10	28
Q96AG4	Leucine-rich repeat-containing protein 59	LRRC59	Homo sapiens	102.16	16.61	4	28
P11177	Pyruvate dehydrogenase E1 component	PDHB	Homo sapiens	107.90	25.35	6	28
	subunit beta, mitochondrial
Q687X5	Metalloreductase STEAP4	STEAP4	Homo sapiens	127.59	20.26	6	28
P10599	Thioredoxin	TXN	Homo sapiens	101.16	48.57	4	28
P13928	Annexin A8	ANXA8	Homo sapiens	98.25	28.44	6	28
O00159	Unconventional myosin-Ic	MYO1C	Homo sapiens	129.53	5.36	3	28
Q9UL46	Proteasome activator complex subunit 2	PSME2	Homo sapiens	132.41	19.67	3	28
O15143	Actin-related protein 2/3 complex subunit 1B	ARPC1B	Homo sapiens	148.32	13.71	3	27
Q9BRX8	Redox-regulatory protein FAM213A	FAM213A	Homo sapiens	72.57	16.59	3	27
O60716	Catenin delta-1	CTNND1	Homo sapiens	99.36	10.95	8	27
Q9UBI6	Guanine nucleotide-binding protein	GNG12	Homo sapiens	104.09	56.94	3	27
	G(I)/G(S)/G(O) subunit gamma-12
P62899	60S ribosomal protein L31	RPL31	Homo sapiens	72.73	18.40	2	27
P62277	40S ribosomal protein S13	RPS13	Homo sapiens	103.45	25.17	4	27
Q7KZF4	Staphylococcal nuclease domain-containing	SND1	Homo sapiens	148.87	15.60	6	27
	protein 1
P50990	T-complex protein 1 subunit theta	CCT8	Homo sapiens	82.21	14.42	6	27
Q53GQ0	Estradiol 17-beta-dehydrogenase 12	HSD17B12	Homo sapiens	109.00	26.60	6	27
P10316	HLA class I histocompatibility antigen, A-69	HLA-A	Homo sapiens	102.31	29.32	7	26
	alpha chain
P30481	HLA class I histocompatibility antigen, B-44	HLA-B	Homo sapiens	106.48	19.89	5	26
	alpha chain
P05090	Apolipoprotein D	APOD	Homo sapiens	79.01	24.34	5	26
P00918	Carbonic anhydrase 2	CA2	Homo sapiens	94.23	23.85	4	26
P17858	6-phosphofructokinase, liver type	PFKL	Homo sapiens	110.94	13.33	5	26
P01598	Ig kappa chain V-I region EU	KV106	Homo sapiens	111.02	26.85	2	26
P08559	Pyruvate dehydrogenase E1 component	PDHA1	Homo sapiens	133.81	18.46	4	26
	subunit alpha, somatic form, mitochondrial
P78527	DNA-dependent protein kinase catalytic subunit	PRKDC	Homo sapiens	124.68	3.83	8	26
Q86TD4	Sarcalumenin	SRL	Homo sapiens	88.38	7.30	6	26
Q15185	Prostaglandin E synthase 3	PTGES3	Homo sapiens	94.89	18.13	2	26
P16070	CD44 antigen	CD44	Homo sapiens	99.10	6.87	4	26
P54709	Sodium/potassium-transporting ATPase	ATP1B3	Homo sapiens	104.69	17.20	3	25
	subunit beta-3
O94905	Erlin-2	ERLIN2	Homo sapiens	77.46	17.99	4	25
Q9BSJ8	Extended synaptotagmin-1	ESYT1	Homo sapiens	90.36	5.89	4	25
Q92597	Protein NDRG1	NDRG1	Homo sapiens	119.80	22.34	5	25
Q9UQ80	Proliferation-associated protein 2G4	PA2G4	Homo sapiens	91.68	17.51	4	25
P42766	60S ribosomal protein L35	RPL35	Homo sapiens	91.31	23.58	3	25
P54920	Alpha-soluble NSF attachment protein	NAPA	Homo sapiens	78.90	15.93	4	25
P59998	Actin-related protein 2/3 complex subunit 4	ARPC4	Homo sapiens	67.76	11.31	2	24
O75947	ATP synthase subunit d, mitochondrial	ATP5H	Homo sapiens	69.10	22.36	3	24
O14958	Calsequestrin-2	CASQ2	Homo sapiens	126.06	28.82	6	24
P13073	Cytochrome c oxidase subunit 4 isoform 1,	COX4I1	Homo sapiens	80.35	19.53	3	24
	mitochondrial
Q16698	2,4-dienoyl-CoA reductase, mitochondrial	DECR1	Homo sapiens	107.00	20.60	4	24
P63167	Dynein light chain 1, cytoplasmic	DYNLL1	Homo sapiens	73.08	49.44	3	24
P16401	Histone H1.5	HIST1H1B	Homo sapiens	72.23	14.60	4	24
P54819	Adenylate kinase 2, mitochondrial	AK2	Homo sapiens	112.91	21.34	3	24
O75489	NADH dehydrogenase [ubiquinone] iron-	NDUFS3	Homo sapiens	90.78	21.97	4	24
	sulfur protein 3, mitochondrial
Q8NC51	Plasminogen activator inhibitor 1 RNA-	SERBP1	Homo sapiens	77.71	13.73	3	24
	binding protein
P62244	40S ribosomal protein S15a	RPS15A	Homo sapiens	118.12	40.00	4	24
P17987	T-complex protein 1 subunit alpha	TCP1	Homo sapiens	72.80	21.40	8	24
P50991	T-complex protein 1 subunit delta	CCT4	Homo sapiens	89.75	5.38	2	24
Q99536	Synaptic vesicle membrane protein VAT-1	VAT1	Homo sapiens	78.40	15.78	4	24
	homolog
P08195	4F2 cell-surface antigen heavy chain	SLC3A2	Homo sapiens	85.62	8.41	4	24
P52179	Myomesin-1	MYOM1	Homo sapiens	92.87	6.94	7	24
P14550	Alcohol dehydrogenase [NADP(+)]	AKR1A1	Homo sapiens	96.16	22.46	4	23
P10768	S-formylglutathione hydrolase	ESD	Homo sapiens	103.70	33.33	5	23
Q12907	Vesicular integral-membrane protein VIP36	LMAN2	Homo sapiens	114.58	27.53	5	23
P26373	60S ribosomal protein L13	RPL13	Homo sapiens	80.03	16.11	3	23
P61353	60S ribosomal protein L27	RPL27	Homo sapiens	68.13	41.91	5	23
P62851	40S ribosomal protein S25	RPS25	Homo sapiens	75.11	24.00	4	23
P26447	Protein S100-A4	S100A4	Homo sapiens	66.34	19.80	2	23
Q9GZM7	Tubulointerstitial nephritis antigen-like	TINAGL1	Homo sapiens	89.95	16.92	4	23
Q13510	Acid ceramidase	ASAH1	Homo sapiens	107.39	10.13	2	23
P05556	Integrin beta-1	ITGB1	Homo sapiens	83.72	12.03	5	23
P11766	Alcohol dehydrogenase class-3	ADH5	Homo sapiens	111.95	31.82	5	22
Q9HDC9	Adipocyte plasma membrane-associated	APMAP	Homo sapiens	140.36	16.35	3	22
	protein
P00915	Carbonic anhydrase 1	CA1	Homo sapiens	83.52	17.62	3	22
Q07065	Cytoskeleton-associated protein 4	CKAP4	Homo sapiens	73.48	22.92	10	22
P53618	Coatomer subunit beta	COPB1	Homo sapiens	101.52	5.35	3	22
Q9Y3I0	tRNA-splicing ligase RtcB homolog	RTCB	Homo sapiens	94.04	16.44	3	22
O43169	Cytochrome b5 type B	CYB5B	Homo sapiens	145.18	36.30	2	22
Q8IZP2	Putative protein FAM10A4	ST13P4	Homo sapiens	100.91	18.33	3	22
P52565	Rho GDP-dissociation inhibitor 1	ARHGDIA	Homo sapiens	90.83	25.00	4	22
Q02978	Mitochondrial 2-oxoglutarate/malate carrier	SLC25A11	Homo sapiens	72.43	22.61	6	22
	protein
P62857	40S ribosomal protein S28	RPS28	Homo sapiens	74.20	33.33	2	22
Q16181	Septin-7	41889	Homo sapiens	127.43	9.38	2	22
P01591	Immunoglobulin J chain	IGJ	Homo sapiens	67.29	23.27	3	22
P23229	Integrin alpha-6	ITGA6	Homo sapiens	115.73	8.05	5	22
Q9BVK6	Transmembrane emp24 domain-containing	TMED9	Homo sapiens	93.53	25.53	4	22
	protein 9
P01019	Angiotensinogen	AGT	Homo sapiens	99.05	11.13	3	21
Q9ULZ3	Apoptosis-associated speck-like protein	PYCARD	Homo sapiens	100.42	15.90	2	21
	containing a CARD
O75531	Barrier-to-autointegration factor	BANF1	Homo sapiens	78.08	40.45	2	21
P61604	10 kDa heat shock protein, mitochondrial	HSPE1	Homo sapiens	79.74	25.49	2	21
P02741	C-reactive protein	CRP	Homo sapiens	58.24	17.41	5	21
P02765	Alpha-2-HS-glycoprotein	AHSG	Homo sapiens	97.90	16.89	3	21
Q14152	Eukaryotic translation initiation factor 3	EIF3A	Homo sapiens	61.17	5.14	5	21
	subunit A
Q9UN36	Protein NDRG2	NDRG2	Homo sapiens	110.96	30.46	5	21
P07225	Vitamin K-dependent protein S	PROS1	Homo sapiens	120.92	6.07	2	21
P62750	60S ribosomal protein L23a	RPL23A	Homo sapiens	62.82	21.15	3	21
P23297	Protein S100-A1	S100A1	Homo sapiens	93.09	23.40	2	21
Q07666	KH domain-containing, RNA-binding, signal	KHDRBS1	Homo sapiens	107.45	7.67	2	21
	transduction-associated protein 1
Q9BS26	Endoplasmic reticulum resident protein 44	ERP44	Homo sapiens	146.67	17.49	4	21
Q96QK1	Vacuolar protein sorting-associated protein	VPS35	Homo sapiens	65.50	7.29	4	21
	35
Q03591	Complement factor H-related protein 1	CFHR1	Homo sapiens	74.21	16.48	4	21
P16144	Integrin beta-4	ITGB4	Homo sapiens	99.62	4.72	4	21
P11279	Lysosome-associated membrane	LAMP1	Homo sapiens	83.40	8.63	3	21
	glycoprotein 1
P27695	DNA-(apurinic or apyrimidinic site) lyase	APEX1	Homo sapiens	94.50	16.98	3	20
Q12797	Aspartyl/asparaginyl beta-hydroxylase	ASPH	Homo sapiens	94.76	7.26	3	20
Q9UGM3	Deleted in malignant brain tumors 1 protein	DMBT1	Homo sapiens	72.30	15.04	3	20
O75874	Isocitrate dehydrogenase [NADP]	IDH1	Homo sapiens	87.46	18.60	5	20
	cytoplasmic
P60866	40S ribosomal protein S20	RPS20	Homo sapiens	80.66	19.33	2	20
P04271	Protein S100-B	S100B	Homo sapiens	130.02	40.22	2	20
P07602	Proactivator polypeptide	PSAP	Homo sapiens	50.52	6.49	3	20
P68036	Ubiquitin-conjugating enzyme E2 L3	UBE2L3	Homo sapiens	111.70	24.03	2	20
P06727	Apolipoprotein A-IV	APOA4	Homo sapiens	57.03	16.67	5	19
Q9NVJ2	ADP-ribosylation factor-like protein 8B	ARL8B	Homo sapiens	89.71	22.58	3	19
P36542	ATP synthase subunit gamma, mitochondrial	ATP5C1	Homo sapiens	51.19	21.48	4	19
P51659	Peroxisomal multifunctional enzyme type 2	HSD17B4	Homo sapiens	102.09	13.45	5	19
O60506	Heterogeneous nuclear ribonucleoprotein Q	SYNCRIP	Homo sapiens	55.74	7.87	4	19
P01765	Ig heavy chain V-III region TIL	HV304	Homo sapiens	90.67	26.09	2	19
P42704	Leucine-rich PPR motif-containing protein,	LRPPRC	Homo sapiens	65.49	6.53	6	19
	mitochondrial
Q53GG5	PDZ and LIM domain protein 3	PDLIM3	Homo sapiens	106.45	15.66	3	19
P46776	60S ribosomal protein L27a	RPL27A	Homo sapiens	52.70	14.86	2	19
P63316	Troponin C, slow skeletal and cardiac	TNNC1	Homo sapiens	70.44	16.15	3	19
	muscles
Q9BXX0	EMILIN-2	EMILIN2	Homo sapiens	66.38	4.94	3	19
Q16836	Hydroxyacyl-coenzyme A dehydrogenase,	HADH	Homo sapiens	91.31	36.62	5	19
	mitochondrial
Q9HC84	Mucin-5B	MUC5B	Homo sapiens	70.98	3.04	4	19
P22061	Protein-L-isoaspartate(D-aspartate) O-	PCMT1	Homo sapiens	122.95	26.43	2	19
	methyltransferase
Q92841	Probable ATP-dependent RNA helicase	DDX17	Homo sapiens	63.73	13.03	6	19
	DDX17
P02656	Apolipoprotein C-III	APOC3	Homo sapiens	81.52	27.27	2	18
P35606	Coatomer subunit beta′	COPB2	Homo sapiens	70.13	12.58	6	18
P13804	Electron transfer flavoprotein subunit alpha,	ETFA	Homo sapiens	55.74	13.81	3	18
	mitochondrial
P01766	Ig heavy chain V-III region BRO	HV305	Homo sapiens	83.54	25.00	2	18
P50213	Isocitrate dehydrogenase [NAD] subunit	IDH3A	Homo sapiens	89.89	17.76	4	18
	alpha, mitochondrial
Q99538	Legumain	LGMN	Homo sapiens	76.81	10.16	2	18
Q04760	Lactoylglutathione lyase	GLO1	Homo sapiens	51.46	29.89	4	18
P55209	Nucleosome assembly protein 1-like 1	NAP1L1	Homo sapiens	71.86	19.95	4	18
P62280	40S ribosomal protein S11	RPS11	Homo sapiens	59.38	31.65	4	18
P62753	40S ribosomal protein S6	RPS6	Homo sapiens	63.34	14.86	3	18
Q14108	Lysosome membrane protein 2	SCARB2	Homo sapiens	80.31	11.09	3	18
P40227	T-complex protein 1 subunit zeta	CCT6A	Homo sapiens	120.87	12.62	3	18
P45974	Ubiquitin carboxyl-terminal hydrolase 5	USP5	Homo sapiens	86.69	8.86	3	18
Q14CN2	Calcium-activated chloride channel regulator	CLCA4	Homo sapiens	68.23	13.06	6	18
	4
Q9UNM6	26S proteasome non-ATPase regulatory	PSMD13	Homo sapiens	80.72	13.03	3	18
	subunit 13
P02730	Band 3 anion transport protein	SLC4A1	Homo sapiens	89.76	10.98	5	17
P34810	Macrosialin	CD68	Homo sapiens	96.51	15.25	2	17
P78417	Glutathione S-transferase omega-1	GSTO1	Homo sapiens	48.66	7.88	2	17
P11940	Polyadenylate-binding protein 1	PABPC1	Homo sapiens	55.95	10.38	5	17
P62913	60S ribosomal protein L11	RPL11	Homo sapiens	56.30	12.92	2	17
P42677	40S ribosomal protein S27	RPS27	Homo sapiens	48.36	28.57	2	17
Q9UHD8	Septin-9	41891	Homo sapiens	69.77	8.70	3	17
P51571	Translocon-associated protein subunit delta	SSR4	Homo sapiens	56.50	24.86	3	17
P40394	Alcohol dehydrogenase class 4 mu/sigma	ADH7	Homo sapiens	124.34	14.51	3	17
	chain
P20073	Annexin A7	ANXA7	Homo sapiens	67.13	9.63	3	17
Q5SSJ5	Heterochromatin protein 1-binding protein 3	HP1BP3	Homo sapiens	64.95	6.87	2	17
P20591	Interferon-induced GTP-binding protein Mx1	MX1	Homo sapiens	88.82	16.47	6	17
P35611	Alpha-adducin	ADD1	Homo sapiens	92.65	8.41	3	16
P24539	ATP synthase subunit b, mitochondrial	ATP5F1	Homo sapiens	57.61	19.53	4	16
P02462	Collagen alpha-1(IV) chain	COL4A1	Homo sapiens	61.11	4.79	5	16
P08246	Neutrophil elastase	ELANE	Homo sapiens	108.82	20.60	2	16
P52566	Rho GDP-dissociation inhibitor 2	ARHGDIB	Homo sapiens	77.52	33.83	3	16
Q1KMD3	Heterogeneous nuclear ribonucleoprotein U-	HNRNPUL2	Homo sapiens	62.82	10.98	4	16
	like protein 2
Q9Y4L1	Hypoxia up-regulated protein 1	HYOU1	Homo sapiens	66.01	9.21	5	16
P05198	Eukaryotic translation initiation factor 2	EIF2S1	Homo sapiens	64.58	23.81	4	16
	subunit 1
Q16795	NADH dehydrogenase [ubiquinone] 1 alpha	NDUFA9	Homo sapiens	51.83	17.77	5	16
	subcomplex subunit 9, mitochondrial
Q14980	Nuclear mitotic apparatus protein 1	NUMA1	Homo sapiens	89.24	2.84	3	16
Q9UMS4	Pre-mRNA-processing factor 19	PRPF19	Homo sapiens	79.00	6.35	2	16
Q15046	Lysine--tRNA ligase	KARS	Homo sapiens	51.73	9.21	3	16
P22735	Protein-glutamine gamma-	TGM1	Homo sapiens	62.57	12.12	5	16
	glutamyltransferase K
Q9Y3B3	Transmembrane emp24 domain-containing	TMED7	Homo sapiens	65.77	19.64	3	16
	protein 7
P47985	Cytochrome b-c1 complex subunit Rieske,	UQCRFS1	Homo sapiens	76.88	17.52	3	16
	mitochondrial
O75339	Cartilage intermediate layer protein 1	CILP	Homo sapiens	49.16	4.65	4	16
P11047	Laminin subunit gamma-1	LAMC1	Homo sapiens	58.80	5.03	5	16
P13796	Plastin-2	LCP1	Homo sapiens	56.95	11.32	5	16
P61160	Actin-related protein 2	ACTR2	Homo sapiens	43.44	8.88	3	15
O15144	Actin-related protein 2/3 complex subunit 2	ARPC2	Homo sapiens	53.85	28.67	6	15
P07358	Complement component C8 beta chain	C8B	Homo sapiens	69.74	8.12	2	15
O75131	Copine-3	CPNE3	Homo sapiens	67.21	11.17	4	15
P22087	rRNA 2′-O-methyltransferase fibrillarin	FBL	Homo sapiens	50.51	15.89	4	15
Q93052	Lipoma-preferred partner	LPP	Homo sapiens	96.40	3.92	2	15
Q9UHQ9	NADH-cytochrome b5 reductase 1	CYB5R1	Homo sapiens	107.11	15.41	2	15
P13667	Protein disulfide-isomerase A4	PDIA4	Homo sapiens	45.77	10.85	5	15
Q15437	Protein transport protein Sec23B	SEC23B	Homo sapiens	64.93	6.52	2	15
P48643	T-complex protein 1 subunit epsilon	CCT5	Homo sapiens	71.26	14.97	4	15
P49419	Alpha-aminoadipic semialdehyde	ALDH7A1	Homo sapiens	47.15	16.33	5	15
	dehydrogenase
P01031	Complement C5	C5	Homo sapiens	60.01	5.19	5	15
O14561	Acyl carrier protein, mitochondrial	NDUFAB1	Homo sapiens	63.69	15.38	2	14
O00499	Myc box-dependent-interacting protein 1	BIN1	Homo sapiens	61.79	14.67	5	14
P30043	Flavin reductase (NADPH)	BLVRB	Homo sapiens	45.07	23.79	3	14
P55290	Cadherin-13	CDH13	Homo sapiens	63.95	4.91	2	14
Q13938	Calcyphosin	CAPS	Homo sapiens	49.97	26.98	4	14
P42126	Enoyl-CoA delta isomerase 1, mitochondrial	ECI1	Homo sapiens	44.26	20.53	4	14
Q9H4M9	EH domain-containing protein 1	EHD1	Homo sapiens	58.11	7.49	2	14
P19367	Hexokinase-1	HK1	Homo sapiens	52.93	9.16	6	14
O60662	Kelch repeat and BTB domain-containing	KLHL41	Homo sapiens	58.11	8.42	5	14
	protein 10
P10620	Microsomal glutathione S-transferase 1	MGST1	Homo sapiens	39.47	18.71	2	14
Q13765	Nascent polypeptide-associated complex	NACA	Homo sapiens	45.56	12.56	2	14
	subunit alpha
O95299	NADH dehydrogenase [ubiquinone] 1 alpha	NDUFA10	Homo sapiens	71.85	13.24	2	14
	subcomplex subunit 10, mitochondrial
Q9UHG3	Prenylcysteine oxidase 1	PCYOX1	Homo sapiens	50.92	12.28	4	14
Q15293	Reticulocalbin-1	RCN1	Homo sapiens	54.12	18.13	4	14
Q07955	Serine/arginine-rich splicing factor 1	SRSF1	Homo sapiens	46.76	17.34	3	14
P30626	Sorcin	SRI	Homo sapiens	52.69	16.16	2	14
Q04837	Single-stranded DNA-binding protein,	SSBP1	Homo sapiens	45.28	32.43	3	14
	mitochondrial
Q9UJZ1	Stomatin-like protein 2	STOML2	Homo sapiens	49.54	17.13	3	14
O75746	Calcium-binding mitochondrial carrier protein	SLC25A12	Homo sapiens	73.38	8.11	2	14
	Aralar1
Q9Y6C2	EMILIN-1	EMILIN1	Homo sapiens	49.93	8.56	6	14
Q07954	Prolow-density lipoprotein receptor-related	LRP1	Homo sapiens	62.18	2.07	5	14
	protein 1
P36952	Serpin B5	SERPINB5	Homo sapiens	55.15	14.13	3	14
P20290	Transcription factor BTF3	BTF3	Homo sapiens	74.92	22.82	2	13
P02745	Complement C1q subcomponent subunit A	C1QA	Homo sapiens	75.24	13.47	2	13
P47756	F-actin-capping protein subunit beta	CAPZB	Homo sapiens	51.06	26.35	4	13
Q9Y678	Coatomer subunit gamma-1	COPG1	Homo sapiens	48.48	11.21	4	13
P05413	Fatty acid-binding protein, heart	FABP3	Homo sapiens	41.42	38.35	4	13
P14314	Glucosidase 2 subunit beta	PRKCSH	Homo sapiens	51.99	11.36	5	13
O00303	Eukaryotic translation initiation factor 3	EIF3F	Homo sapiens	53.04	19.05	4	13
	subunit F
P04434	Ig kappa chain V-III region VH (Fragment)	KV310	Homo sapiens	33.54	23.28	2	13
O00232	26S proteasome non-ATPase regulatory	PSMD12	Homo sapiens	45.14	7.46	2	13
	subunit 12
P98179	Putative RNA-binding protein 3	RBM3	Homo sapiens	66.79	31.85	2	13
P21589	5′-nucleotidase	NT5E	Homo sapiens	54.86	9.23	2	12
P00966	Argininosuccinate synthase	ASS1	Homo sapiens	69.29	10.19	2	12
P08571	Monocyte differentiation antigen CD14	CD14	Homo sapiens	50.36	14.40	3	12
Q9ULV4	Coronin-1C	CORO1C	Homo sapiens	75.32	13.29	2	12
P21912	Succinate dehydrogenase [ubiquitone] iron-	SDHB	Homo sapiens	44.99	10.71	2	12
	sulfur subunit, mitochondrial
P35573	Glycogen debranching enzyme	AGL	Homo sapiens	60.07	3.26	3	12
Q96AB3	Isochorismatase domain-containing protein 2,	ISOC2	Homo sapiens	55.91	25.85	2	12
	mitochondrial
P30085	UMP-CMP kinase	CMPK1	Homo sapiens	56.96	22.96	3	12
P80188	Neutrophil gelatinase-associated lipocalin	LCN2	Homo sapiens	35.66	26.77	3	12
P61970	Nuclear transport factor 2	NUTF2	Homo sapiens	70.89	33.86	2	12
O75915	PRA1 family protein 3	ARL6IP5	Homo sapiens	42.87	15.96	2	12
P62491	Ras-related protein Rab-11A	RAB11A	Homo sapiens	36.42	16.20	3	12
P84098	60S ribosomal protein L19	RPL19	Homo sapiens	35.15	30.10	6	12
Q01105	Protein SET	SET	Homo sapiens	69.40	17.24	2	12
P39687	Acidic leucine-rich nuclear phosphoprotein 32	ANP32A	Homo sapiens	38.78	14.06	3	11
	family member A
Q96CW1	AP-2 complex subunit mu	AP2M1	Homo sapiens	50.41	11.72	3	11
P02652	Apolipoprotein A-II	APOA2	Homo sapiens	30.45	39.00	3	11
P18859	ATP synthase-coupling factor 6,	ATP5J	Homo sapiens	45.89	30.56	2	11
	mitochondrial
Q99715	Collagen alpha-1(XII) chain	COL12A1	Homo sapiens	42.85	1.99	4	11
Q9Y394	Dehydrogenase/reductase SDR family	DHRS7	Homo sapiens	51.65	16.52	3	11
	member 7
O43143	Putative pre-mRNA-splicing factor ATP-	DHX15	Homo sapiens	35.61	4.28	2	11
	dependent RNA helicase DHX15
P11166	Solute carrier family 2, facilitated glucose	SLC2A1	Homo sapiens	29.34	6.30	3	11
	transporter member 1
P38159	RNA-binding motif protein, X chromosome	RBMX	Homo sapiens	31.82	5.37	2	11
P01714	Ig lambda chain V-III region SH	LV301	Homo sapiens	37.60	25.00	2	11
P23368	NAD-dependent malic enzyme, mitochondrial	ME2	Homo sapiens	64.29	15.07	3	11
Q9Y6C9	Mitochondrial carrier homolog 2	MTCH2	Homo sapiens	42.05	16.83	3	11
P49821	NADH dehydrogenase [ubiquinone]	NDUFV1	Homo sapiens	40.34	17.24	4	11
	flavoprotein 1, mitochondrial
Q9Y2J8	Protein-arginine deiminase type-2	PADI2	Homo sapiens	55.16	5.56	2	11
P01833	Polymeric immunoglobulin receptor	PIGR	Homo sapiens	59.96	4.84	2	11
P61019	Ras-related protein Rab-2A	RBS2A	Homo sapiens	38.60	12.74	2	11
P40763	Signal transducer and activator of	STAT3	Homo sapiens	75.61	8.70	3	11
	transcription 3
Q15363	Transmembrane emp24 domain-containing protein 2	TMED2	Homo sapiens	37.40	18.41	3	11
P07998	Thrombospondin-1	THBS1	Homo sapiens	51.87	2.74	2	11
O75643	U5 small nuclear ribonucleoprotein 200 kDa	SNRNP200	Homo sapiens	58.36	4.21	4	11
	helicase
Q15029	116 kDa U5 small nuclear ribonucleoprotein	EFTUD2	Homo sapiens	69.20	3.81	2	11
	component
Q9UH99	SUN domain-containing protein 2	SUN2	Homo sapiens	49.31	10.46	4	11
O75533	Splicing factor 3B subunit 1	SF3B1	Homo sapiens	48.28	2.45	2	11
P46977	Dolichyl-diphosphooligosaccharide--protein	STT3A	Homo sapiens	64.42	5.82	2	11
	glycosyltransferase subunit STT3A
Q16658	Fascin	FSCN1	Homo sapiens	51.61	8.11	2	10
Q9NZ01	Very-long-chain enoyl-CoA reductase	TECR	Homo sapiens	36.79	9.42	2	10
P08263	Glutathione S-transferase A1	GSTA1	Homo sapiens	28.39	17.57	3	10
Q13751	Laminin subunit beta-3	LAMB3	Homo sapiens	41.29	6.06	4	10
P37837	Transaldolase	TALDO1	Homo sapiens	32.08	14.54	4	10
Q6ZMU5	Tripartite motif-containing protein 72	TRIM72	Homo sapiens	52.07	14.47	4	10
P53634	Dipeptidyl peptidase 1	CTSC	Homo sapiens	42.71	12.31	3	10
P14543	Nidogen-1	NID1	Homo sapiens	41.25	5.53	4	10
Q9NYU2	UDP-glucose: glycoprotein glucosyltransferase 1	UGGT1	Homo sapiens	34.11	6.88	5	10
B0FP48	Uroplakin-3b-like protein	UPK3BL	Homo sapiens	29.21	13.31	3	10
P61163	Alpha-centractin	ACTR1A	Homo sapiens	38.24	10.11	2	9
P04745	Alpha-amylase 1	AMY1A	Homo sapiens	49.22	15.07	4	9
P51572	B-cell receptor-associated protein 31	BCAP31	Homo sapiens	25.93	21.54	4	9
P24592	Insulin-like growth factor-binding protein 6	IGFBP6	Homo sapiens	48.09	18.33	2	9
Q13421	Mesothelin	MSLN	Homo sapiens	42.32	6.35	2	9
P22307	Non-specific lipid-transfer protein	SCP2	Homo sapiens	26.93	6.95	3	9
P55786	Puromycin-sensitive aminopeptidase	NPEPPS	Homo sapiens	30.12	6.42	3	9
P61254	60S ribosomal protein L26	RPL26	Homo sapiens	23.65	17.93	3	9
P67812	Signal peptidase complex catalytic subunit	SEC11A	Homo sapiens	22.32	15.64	3	9
	SEC11A
Q5VXT5	Synaptophysin-like protein 2	SYPL2	Homo sapiens	33.05	10.66	2	9
P13693	Translationally-controlled tumor protein	TPT1	Homo sapiens	31.41	12.21	2	9
Q9UDW1	Cytochrome b-c1 complex subunit 9	UQCR10	Homo sapiens	29.74	38.10	2	9
Q6YHK3	CD109 antigen	CD109	Homo sapiens	30.44	3.88	3	8
Q9Y224	UPF0568 protein C14orf166	C14orf166	Homo sapiens	41.55	17.62	3	8
Q16610	Extracellular matrix protein 1	ECM1	Homo sapiens	35.88	6.67	2	8
Q13347	Eukaryotic translation initiation factor 3	EIF3I	Homo sapiens	36.42	16.31	3	8
	subunit I
P40261	Nicotinamide N-methyltransferase	NNMT	Homo sapiens	39.05	12.12	2	8
O75340	Programmed cell death protein 6	PDCD6	Homo sapiens	25.68	12.57	2	8
P62714	Serine/threonine-protein phosphatase 2A	PPP2CB	Homo sapiens	42.30	12.94	2	8
	catalytic subunit beta isoform
P28066	Proteasome subunit alpha type-5	PSMA5	Homo sapiens	35.94	29.88	4	8
P61224	Ras-related protein Rap-1b	RAP1B	Homo sapiens	25.05	26.09	4	8
Q9Y265	RuvB-like 1	RUVBL1	Homo sapiens	26.51	8.99	3	8
P61619	Protein transport protein Sec61 subunit alpha	SEC61A1	Homo sapiens	28.34	6.51	2	8
	isoform 1
Q8TCJ2	Dolichyl-diphosphooligosaccharide--protein	STT3B	Homo sapiens	38.82	3.51	2	8
	glycosyltransferase subunit STT3B
Q92896	Golgi apparatus protein 1	GLG1	Homo sapiens	48.26	4.92	3	8
	Dihydrolipoyllysine-residue acetyltransferase
P10515	component of pyruvate dehydrogenase	DLAT	Homo sapiens	31.01	10.51	4	8
	complex, mitochondrial
Q6NUK1	Calcium-binding mitochondrial carrier protein	SLC25A24	Homo sapiens	21.03	6.92	3	8
	SCaMC-1
O43488	Aflatoxin B1 aldehyde reductase member 2	AKR7A2	Homo sapiens	44.29	13.37	2	7
O15511	Actin-related protein 2/3 complex subunit 5	ARPC5	Homo sapiens	27.05	21.19	2	7
P34897	Serine hydroxymethyltransferase,	SHMT2	Homo sapiens	18.65	5.36	2	7
	mitochondrial
P07305	Histone H1.0	H1F0	Homo sapiens	17.16	11.34	2	7
Q12906	Interleukin enhancer-binding factor 3	ILF3	Homo sapiens	21.77	4.47	3	7
Q08380	Galectin-3-binding protein	LGALS3BP	Homo sapiens	29.58	4.96	2	7
P16050	Arachidonate 15-lipoxygenase	ALOX15	Homo sapiens	33.24	6.19	2	7
P01700	Ig lambda chain V-I region HA	LV102	Homo sapiens	19.51	18.75	2	7
Q99972	Myocitin	MYOC	Homo sapiens	20.28	10.91	4	7
Q96AC1	Fermitin family homolog 2	FERMT2	Homo sapiens	22.01	5.00	2	7
Q9Y446	Plakophilin-3	PKP3	Homo sapiens	25.64	6.15	3	7
P25786	Proteasome subunit alpha type-1	PSMA1	Homo sapiens	32.33	19.77	3	7
Q99460	26S proteasome non-ATPase regulatory	PSMD1	Homo sapiens	30.78	5.98	3	7
	subunit 1
P06703	Protein S100-A6	S100A6	Homo sapiens	24.60	40.00	3	7
P23526	Adenosylhomocysteinase	AHCY	Homo sapiens	25.52	10.65	3	7
Q96PL1	Secretoglobin family 3A member 2	SCGB3A2	Homo sapiens	27.23	33.33	2	7
P54577	Tyrosine--tRNA ligase, cytoplasmic	YARS	Homo sapiens	34.21	10.61	3	7
O60701	UDP-glucose 6-dehydrogenase	UGDH	Homo sapiens	22.69	8.30	2	7
P40121	Macrophage-capping protein	CAPG	Homo sapiens	27.36	13.51	3	7
Q7L2H7	Eukaryotic translation initiation factor 3	EIF3M	Homo sapiens	34.24	12.57	3	7
	subunit M
Q6YN16	Hydroxysteriod dehydrogenase-like protein 2	HSDL2	Homo sapiens	27.28	8.85	2	7
Q969G5	Protein kinase C delta-binding protein	PRKCDBP	Homo sapiens	24.98	13.79	3	7
P35613	Basigin	BSG	Homo sapiens	25.29	8.31	2	6
P09871	Complement C1s subcomponent	C1S	Homo sapiens	23.20	4.36	2	6
Q92499	ATP-dependent RNA helicase DDX1	DDX1	Homo sapiens	28.65	6.89	3	6
Q9UBX5	Fibulin-5	FBLN5	Homo sapiens	19.62	6.70	2	6
O75955	Flotillin-1	FLOT1	Homo sapiens	20.47	7.73	2	6
P21695	Glycerol-3-phosphate dehydrogenase	GPD1	Homo sapiens	27.44	22.64	4	6
	[NAD(+)], cytoplasmic
O14558	Heat shock protein beta-6	HSPB6	Homo sapiens	23.37	21.88	2	6
P20810	Calpastatin	CAST	Homo sapiens	25.02	6.21	2	6
Q12905	Interleukin enhancer-binding factor 2	ILF2	Homo sapiens	23.04	18.97	4	6
P48729	Casein kinase I isoform alpha	CSNK1A1	Homo sapiens	15.74	6.53	2	6
P80748	Ig lambda chain V-III region LOI	LV302	Homo sapiens	19.76	21.62	2	6
P51970	NADR dehydrogenase [ubiquinone] 1 alpha	NDUFA8	Homo sapiens	21.24	29.07	3	6
	subcomplex subunit 8
Q6P2Q9	Pre-mRNA-processing-splicing factor 8	PRPF8	Homo sapiens	27.11	1.88	2	6
P62333	26S protease regulatory subunit 10B	PSMC6	Homo sapiens	22.70	7.97	2	6
P63000	Ras-related C3 botulinum toxin substrate 1	RAC1	Homo sapiens	16.60	22.40	4	6
Q15393	Splicing factor 3B subunit 3	SF3B3	Homo sapiens	28.58	3.20	2	6
O75368	SH3 domain-binding glutamic acid-rich-like	SH3BGRL	Homo sapiens	16.99	27.19	2	6
	protein
P26640	Valine--tRNA ligase	VARS	Homo sapiens	25.03	2.37	2	6
P61421	V-type proton ATPase subunit d 1	ATP3V0D1	Homo sapiens	20.75	10.26	2	6
Q14254	Flotillin-2	FLOT2	Homo sapiens	22.29	7.24	2	6
P27169	Serum paraoxonase/arylesterase 1	PON1	Homo sapiens	19.01	21.97	3	6
P04275	von Willebrand factor	VWF	Homo sapiens	18.91	1.03	2	6
P51858	Hepatoma-derived growth factor	HDGF	Homo sapiens	21.84	14.17	2	5
Q96CX2	BTB/POZ domain-containing protein KCTD12	KCTD12	Homo sapiens	13.69	7.08	2	5
Q13753	Laminin subunit gamma-2	LAMC2	Homo sapiens	29.52	4.11	2	5
P43243	Matrin-3	MATR3	Homo sapiens	15.97	3.78	2	5
Q9NP98	Myozenin-1	MYOZ1	Homo sapiens	25.04	11.04	2	5
Q9P2E9	Ribosome-binding protein 1	RRBP1	Homo sapiens	13.24	2.34	3	5
P49458	Signal recognition particle 9 kDa protein	SRP9	Homo sapiens	14.05	23.26	2	5
P42224	Signal transducer and activator of	STAT1	Homo sapiens	18.45	4.00	2	5
	transcription 1-alpha/beta
O14907	Tax1-binding protein 3	TAX1BP3	Homo sapiens	18.96	28.23	2	5
Q04446	1,4-alpha-glucan-branching enzyme	GBE1	Homo sapiens	20.75	5.13	2	5
O15230	Laminin subunit alpha-5	LAMA5	Homo sapiens	29.62	1.35	2	5
Q00341	Vigilin	HDLBP	Homo sapiens	24.79	3.08	2	5
P30049	ATP synthase subunit delta, mitochondrial	ATP5D	Homo sapiens	12.28	13.69	2	4
P23141	Liver carboxylesterase 1	CES1	Homo sapiens	14.18	5.47	2	4
Q9UBQ7	Glyoxylate reductase/hydroxypyruvate	GRHPR	Homo sapiens	17.86	12.20	2	4
	reductase
P07686	Beta-hexosaminidase subunit beta	HEXB	Homo sapiens	18.55	8.63	2	4
P05455	Lupus La protein	SSB	Homo sapiens	17.39	10.78	3	4
P19404	NADH dehydrogenase [ubiquinone]	NDUFV2	Homo sapiens	10.94	9.64	2	4
	flavoprotein 2, mitochondrial
Q13423	NAD(P) transhydrogenase, mitochondrial	NNT	Homo sapiens	21.45	3.41	2	4
P04181	Ornithine aminotransferase, mitochondrial	OAT	Homo sapiens	11.54	7.29	2	4
P11498	Pyruvate carboxylase, mitochondrial	PC	Homo sapiens	24.43	3.40	2	4
Q00765	Receptor expression-enhancing protein 5	REEP5	Homo sapiens	11.75	10.05	2	4
Q9NVA2	Septin-11	41893	Homo sapiens	9.98	4.30	2	4
Q01130	Serine/arginine-rich splicing factor 2	SRSF2	Homo sapiens	13.67	12.67	2	4
P38606	V-type proton ATPase catalytic subunit A	ATP6V1A	Homo sapiens	15.43	7.46	3	4
O14980	Exportin-1	XPO1	Homo sapiens	21.82	3.27	2	4
Q8IVF2	Protein AHNAK2	AHNAK2	Homo sapiens	11.71	5.18	2	4
Q9UBG3	Cornulin	CRNN	Homo sapiens	15.63	16.77	4	4
Q06033	Inter-alpha-trypsin inhibitor heavy chain H3	ITIH3	Homo sapiens	20.77	5.51	2	4
Q16787	Laminin subunit alpha-3	LAMA3	Homo sapiens	25.74	1.32	2	4
P62330	ADP-ribosylation factor 6	ARF6	Homo sapiens	11.13	20.00	2	3
P50402	Emerin	EMD	Homo sapiens	11.98	16.54	2	3
P15104	Glutamine synthetase	GLUL	Homo sapiens	11.91	9.92	2	3
P10644	cAMP-dependent protein kinase type I-alpha	PRKAR1A	Homo sapiens	13.14	7.87	2	3
	regulatory subunit
P13473	Lysosome-associated membrane	LAMP2	Homo sapiens	9.53	4.88	2	3
	glycoprotein 2
Q9BQ69	O-acetyl-ADP-ribose deacetylase MACROD1	MACROD1	Homo sapiens	15.78	11.38	2	3
P53007	Tricarboxylate transport protein,	SLC25A1	Homo sapiens	13.07	15.11	2	3
	mitochondrial
P49327	Fatty acid synthase	FASN	Homo sapiens	13.24	2.35	3	3
P07476	Involucrin	IVL	Homo sapiens	14.25	6.84	2	3
Q5VTT5	Myomesin-3	MYOM3	Homo sapiens	12.97	4.73	3	3
P07814	Bifunctional glutamate/prolone--tRNA ligase	EPRS	Homo sapiens	17.22	3.24	2	3
P12830	Cadherin-1	CDH1	Homo sapiens	6.40	4.08	2	2
P04040	Catalase	CAT	Homo sapiens	8.25	6.45	2	2
Q13217	DnaJ homolog subfamily C member 3	DNAJC3	Homo sapiens	9.96	9.72	2	2
Q9UBY9	Heat shock protein beta-7	HSPB7	Homo sapiens	6.55	18.82	2	2
Q9Y262	Eukaryotic translation initiation factor 3	EIF3L	Homo sapiens	9.03	4.26	2	2
	subunit L
Q92804	TATA-binding protein-associated factor 2N	TAF15	Homo sapiens	5.57	4.39	2	2
P02753	Retinol-binding protein 4	RBP4	Homo sapiens	6.32	9.95	2	2
Q8NB12	SET and MYND domain-containing protein 1	SMYD1	Homo sapiens	5.69	6.53	2	2
Q9NSE4	Isoleucine--tRNA ligase, mitochondrial	IARS2	Homo sapiens	6.92	2.77	2	2
Q9HD45	Transmembrane 9 superfamily member 3	TM9SF3	Homo sapiens	6.34	5.26	2	2
Q9P0L0	Vesicle-associated membrane protein-	VAPA	Homo sapiens	8.47	14.06	2	2
	associated protein A

TABLE 2
Gene ontology (biological process, cellular component and molecular function) terms enriched in the engineered VF mucosa proteome.
Enriched ontology terms were identified using the BINGO algorithm. The term list shown here corresponds to the functional
enrichment map presented in FIG. 3E. Terms are listed in order of ascending Benjamini Hochberg-adjusted P-value.
Gene ontology term	Description	Adjusted P-value
GO:44444	cytoplasmic part	0.00E+00
GO:5737	cytoplasm	0.00E+00
GO:5829	cytosol	0.00E+00
GO:44424	intracellular part	0.00E+00
GO:44422	organelle part	0.00E+00
GO:44446	intracellular organelle part	0.00E+00
GO:5515	protein binding	0.00E+00
GO:5622	intracellular	0.00E+00
GO:43226	organelle	0.00E+00
GO:43229	intracellular organelle	0.00E+00
GO:44464	cell part	0.00E+00
GO:5623	cell	0.00E+00
GO:16043	cellular component organization	0.00E+00
GO:71840	cellular component organization or biogenesis	0.00E+00
GO:43227	membrane-bounded organelle	1.54E−98
GO:9056	catabolic process	1.32E−97
GO:32991	macromolecular complex	7.91E−96
GO:43231	intracellular membrane-bounded organelle	2.82E−92
GO:1901575	organic substance catabolic process	1.10E−90
GO:31974	membrane-enclosed lumen	6.02E−90
GO:43233	organelle lumen	3.94E−89
GO:44248	cellular catabolic process	5.02E−86
GO:5575	cellular component	6.23E−84
GO:44281	small molecule metabolic process	1.14E−83
GO:16071	mRNA metabolic process	1.40E−83
GO:70013	intracellular organelle lumen	8.21E−83
GO:16032	viral reproduction	4.94E−82
GO:44764	multi-organism cellular process	6.49E−82
GO:22411	cellular component disassembly	1.09E−77
GO:19083	viral transcription	2.90E−77
GO:44710	single-organism metabolic process	3.71E−77
GO:9987	cellular process	5.80E−77
GO:43933	macromolecular complex subunit organization	6.37E−75
GO:44237	cellular metabolic process	1.39E−74
GO:22415	viral reproductive process	8.03E−74
GO:19080	viral genome expression	1.63E−73
GO:6614	SRP-dependent cotranslational protein targeting to membrane	3.08E−73
GO:22626	cytosolic ribosome	3.50E−72
GO:19058	viral infectious cycle	3.83E−72
GO:45047	protein targeting to ER	8.85E−72
GO:6414	translational elongation	1.01E−71
GO:72599	establishment of protein localization to endoplasmic reticulum	1.59E−71
GO:6613	cotranslational protein targeting to membrane	1.59E−71
GO:6415	translational termination	2.24E−71
GO:71822	protein complex subunit organization	3.93E−71
GO:184	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay	6.64E−71
GO:70972	protein localization to endoplasmic reticulum	1.49E−70
GO:5488	binding	6.41E−69
GO:43624	cellular protein complex disassembly	3.58E−68
GO:71704	organic substance metabolic process	4.71E−68
GO:43241	protein complex disassembly	3.35E−66
GO:44699	single-organism process	3.35E−66
GO:51649	establishment of localization in cell	4.99E−66
GO:51641	cellular localization	7.77E−66
GO:6612	protein targeting to membrane	5.56E−64
GO:32984	macromolecular complex disassembly	2.81E−63
GO:31988	membrane-bounded vesicle	4.70E−63
GO:43228	non-membrane-bounded organelle	8.25E−63
GO:43232	intracellular non-membrane-bounded organelle	8.25E−63
GO:31982	vesicle	1.13E−62
GO:44238	primary metabolic process	1.22E−62
GO:44703	multi-organism reproductive process	4.22E−62
GO:46700	heterocycle catabolic process	1.06E−61
GO:34655	nucleobase-containing compound catabolic process	1.21E−61
GO:6402	mRNA catabolic process	2.73E−61
GO:46907	intracellular transport	3.34E−61
GO:30529	ribonucleoprotein complex	3.34E−61
GO:6413	translational initiation	3.74E−61
GO:1901361	organic cyclic compound catabolic process	4.66E−61
GO:44270	cellular nitrogen compound catabolic process	5.09E−61
GO:956	nuclear-transcribed mRNA catabolic process	5.26E−61
GO:44763	single-organism cellular process	5.79E−61
GO:19439	aromatic compound catabolic process	8.81E−61
GO:51704	multi-organism process	1.33E−60
GO:16482	cytoplasmic transport	1.49E−59
GO:44445	cytosolic part	1.05E−58
GO:33036	macromolecule localization	2.01E−58
GO:6401	RNA catabolic process	2.63E−58
GO:45184	establishment of protein localization	9.90E−58
GO:8104	protein localization	1.15E−57
GO:6807	nitrogen compound metabolic process	1.41E−57
GO:72594	establishment of protein localization to organelle	5.33E−57
GO:15031	protein transport	6.04E−57
GO:5198	structural molecule activity	6.40E−57
GO:6605	protein targeting	1.79E−56
GO:9057	macromolecule catabolic process	3.33E−56
GO:8152	metabolic process	1.14E−55
GO:42470	melanosome	1.61E−55
GO:48770	pigment granule	1.61E−55
GO:44391	ribosomal subunit	3.17E−54
GO:34641	cellular nitrogen compound metabolic process	2.68E−53
GO:6886	intracellular protein transport	7.01E−53
GO:34613	cellular protein localization	5.66E−52
GO:70727	cellular macromolecule localization	7.49E−52
GO:33365	protein localization to organelle	3.51E−51
GO:71702	organic substance transport	3.13E−50
GO:44265	cellular macromolecule catabolic process	4.07E−50
GO:70062	extracellular vesicular exosome	1.53E−49
GO:43230	extracellular organelle	6.40E−49
GO:65010	extracellular membrane-bounded organelle	6.40E−49
GO:1901360	organic cyclic compound metabolic process	1.66E−48
GO:43234	protein complex	9.14E−47
GO:51179	localization	3.29E−46
GO:6412	translation	3.94E−45
GO:6139	nucleobase-containing compound metabolic process	6.37E−45
GO:44765	single-organism transport	7.39E−45
GO:6725	cellular aromatic compound metabolic process	1.36E−44
GO:46483	heterocycle metabolic process	2.02E−44
GO:6810	transport	1.09E−43
GO:16023	cytoplasmic membrane-bounded vesicle	1.22E−43
GO:51234	establishment of localization	1.39E−43
GO:6950	response to stress	4.03E−43
GO:31410	cytoplasmic vesicle	7.10E−43
GO:3674	molecular_function	1.05E−41
GO:22414	reproductive process	2.79E−41
GO:3	reproduction	4.55E−41
GO:22625	cytosolic large ribosomal subunit	6.24E−41
GO:1901564	organonitrogen compound metabolic process	6.68E−40
GO:65008	regulation of biological quality	2.85E−39
GO:8150	biological_process	4.53E−39
GO:3723	RNA binding	1.55E−38
GO:31090	organelle membrane	5.16E−38
GO:44085	cellular component biogenesis	1.31E−37
GO:5739	mitochondrion	4.26E−36
GO:48519	negative regulation of biological process	6.90E−36
GO:50896	response to stimulus	9.53E−36
GO:43170	macromolecule metabolic process	8.41E−35
GO:19538	protein metabolic process	1.33E−34
GO:9058	biosynthetic process	1.60E−34
GO:48518	positive regulation of biological process	1.91E−34
GO:42221	response to chemical stimulus	2.82E−34
GO:6091	generation of precursor metabolites and energy	2.92E−34
GO:6996	organelle organization	7.00E−34
GO:1901576	organic substance biosynthetic process	1.24E−33
GO:44267	cellular protein metabolic process	1.59E−33
GO:22607	cellular component assembly	2.25E−33
GO:22627	cytosolic small ribosomal subunit	2.29E−33
GO:10467	gene expression	3.90E−33
GO:48522	positive regulation of cellular process	9.45E−33
GO:48523	negative regulation of cellular process	2.65E−32
GO:44428	nuclear part	4.50E−32
GO:5786	endoplasmic reticulum lumen	5.08E−32
GO:10941	regulation of cell death	1.06E−31
GO:1901135	carbohydrate derivative metabolic process	1.90E−31
GO:43067	regulation of programmed cell death	2.42E−31
GO:42961	regulation of apoptotic process	4.67E−31
GO:65003	macromolecular complex assembly	7.55E−31
GO:8219	cell death	1.01E−30
GO:2478	antigen processing and presentation of exogenous peptide antigen	1.13E−30
GO:16265	death	1.55E−30
GO:5783	endoplasmic reticulum	2.16E−30
GO:19884	antigen processing and presentation of exogenous antigen	2.36E−30
GO:5840	ribosome	4.50E−30
GO:48002	antigen processing and presentation of peptide antigen	4.87E−30
GO:6082	organic acid metabolic process	6.10E−30
GO:15934	large ribosomal subunit	6.56E−30
GO:55114	oxidation-reduction process	6.89E−30
GO:43436	oxoacid metabolic process	1.21E−29
GO:44249	cellular biosynthetic process	1.46E−29
GO:44260	cellular macromolecule metabolic process	1.92E−29
GO:1901565	organonitrogen compound catabolic process	2.57E−29
GO:3735	structural constituent of ribosome	3.22E−29
GO:31981	nuclear lumen	9.80E−29
GO:70887	cellular response to chemical stimulus	1.14E−28
GO:19752	carboxylic acid metabolic process	1.39E−28
GO:16070	RNA metabolic process	2.30E−28
GO:2376	immune system process	3.98E−28
GO:44421	extracellular region part	5.42E−28
GO:51701	interaction with host	1.05E−27
GO:44432	endoplasmic reticulum part	1.06E−27
GO:5856	cytoskeleton	2.82E−27
GO:51246	regulation of protein metabolic process	3.88E−27
GO:55086	nucleobase-containing small molecule metabolic process	5.77E−27
GO:10033	response to organic substance	8.11E−27
GO:44419	interspecies interaction between organisms	1.16E−26
GO:44403	symbiosis, encompassing mutualism through parasitism	1.16E−26
GO:48610	cellular process involved in reproduction	1.49E−26
GO:44707	single-multicellular organism process	1.76E−26
GO:15980	energy derivation by oxidation of organic compounds	1.98E−26
GO:32268	regulation of cellular protein metabolic process	2.06E−26
GO:19048	virus-host interaction	4.98E−26
GO:9117	nucleotide metabolic process	5.91E−26
GO:44429	mitochondrial part	7.15E−26
GO:90304	nucleic acid metabolic process	7.67E−26
GO:6753	nucleoside phosphate metabolic process	1.43E−25
GO:7599	hemostasis	2.09E−25
GO:32501	multicellular organismal process	5.52E−25
GO:65007	biological regulation	6.18E−25
GO:31967	organelle envelope	1.01E−24
GO:16044	cellular membrane organization	1.04E−24
GO:16192	vesicle-mediated transport	1.63E−24
GO:61024	membrane organization	1.97E−24
GO:6521	regulation of cellular amino acid metabolic process	2.66E−24
GO:2474	antigen processing and presentation of peptide antigen via MHC class I	4.07E−24
GO:1901136	carbohydrate derivative catabolic process	4.47E−24
GO:15935	small ribosomal subunit	4.47E−24
GO:31975	envelope	5.21E−24
GO:50817	coagulation	5.86E−24
GO:7596	blood coagulation	5.86E−24
GO:51128	regulation of cellular component organization	6.44E−24
GO:5634	nucleus	1.10E−23
GO:6457	protein folding	1.64E−23
GO:5759	mitochondrial matrix	2.58E−23
GO:30198	extracellular matrix organization	3.21E−23
GO:43062	extracellular structure organization	3.57E−23
GO:19882	antigen processing and presentation	4.64E−23
GO:42060	wound healing	1.15E−22
GO:50878	regulation of body fluid levels	2.65E−22
GO:12505	endomembrane system	3.32E−22
GO:42802	identical protein binding	3.74E−22
GO:19637	organophosphate metabolic process	4.07E−22
GO:6461	protein complex assembly	4.58E−22
GO:70271	protein complex biogenesis	5.33E−22
GO:33238	regulation of cellular amine metabolic process	1.01E−21
GO:50789	regulation of biological process	1.02E−21
GO:12501	programmed cell death	1.48E−21
GO:51436	negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle	1.52E−21
GO:6163	purine nucleotide metabolic process	3.11E−21
GO:10608	posttranscriptional regulation of gene expression	3.26E−21
GO:72413	signal transduction involved in mitotic cell cycle checkpoint	4.50E−21
GO:72431	signal transduction involved in mitotic G1 DNA damage checkpoint	4.50E−21
GO:6977	DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest	4.50E−21
GO:72401	signal transduction involved in DNA integrity checkpoint	6.36E−21
GO:72422	signal transduction involved in DNA damage checkpoint	6.36E−21
GO:72521	purine-containing compound metabolic process	6.79E−21
GO:1901657	glycosyl compound metabolic process	7.55E−21
GO:6915	apoptotic process	8.15E−21
GO:51437	positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle	8.83E−21
GO:72395	signal transduction involved in cell cycle checkpoint	8.83E−21
GO:30330	DNA damage response, signal transduction by p53 class mediator	1.01E−20
GO:51443	positive regulation of ubiquitin-protein ligase activity	1.22E−20
GO:9059	macromolecule biosynthetic process	1.40E−20
GO:46128	purine ribonucleoside metabolic process	1.88E−20
GO:42278	purine nucleoside metabolic process	2.12E−20
GO:9150	purine ribonucleotide metabolic process	2.60E−20
GO:19693	ribose phosphate metabolic process	2.60E−20
GO:6417	regulation of translation	3.49E−20
GO:51351	positive regulation of ligase activity	3.91E−20
GO:51352	negative regulation of ligase activity	4.43E−20
GO:51444	negative regulation of ubiquitin-protein ligase activity	4.43E−20
GO:2479	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-	4.55E−20
	dependent
GO:44433	cytoplasmic vesicle part	4.95E−20
GO:72331	signal transduction by p53 class mediator	5.62E−20
GO:51439	regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle	5.98E−20
GO:72523	purine-containing compound catabolic process	7.54E−20
GO:44092	negative regulation of molecular function	7.54E−20
GO:9259	ribonucleotide metabolic process	7.56E−20
GO:19899	enzyme binding	8.81E−20
GO:42590	antigen processing and presentation of exogenous peptide antigen via MHC class I	1.05E−19
GO:44783	G1 DNA damage checkpoint	1.08E−19
GO:31571	mitotic G1 DNA damage checkpoint	1.08E−19
GO:9119	ribonucleoside metabolic process	1.19E−19
GO:9611	response to wounding	1.38E−19
GO:6195	purine nucleotide catabolic process	1.86E−19
GO:2000134	negative regulation of G1/S transition of mitotic cell cycle	1.94E−19
GO:9116	nucleoside metabolic process	1.95E−19
GO:6152	purine nucleoside catabolic process	2.05E−19
GO:46130	purine ribonucleoside catabolic process	2.05E−19
GO:36094	small molecule binding	2.44E−19
GO:42770	signal transduction in response to DNA damage	2.61E−19
GO:44430	cytoskeletal part	3.07E−19
GO:42454	ribonucleoside catabolic process	3.11E−19
GO:71944	cell periphery	3.38E−19
GO:43086	negative regulation of catalytic activity	3.86E−19
GO:2576	platelet degranulation	4.41E−19
GO:9164	nucleoside catabolic process	5.11E−19
GO:48856	anatomical structure development	5.49E−19
GO:71158	positive regulation of cell cycle arrest	5.77E−19
GO:1901658	glycosyl compound catabolic process	6.25E−19
GO:50794	regulation of cellular process	6.81E−19
GO:51248	negative regulation of protein metabolic process	1.03E−18
GO:32502	developmental process	1.05E−18
GO:51716	cellular response to stimulus	1.12E−18
GO:9166	nucleotide catabolic process	1.18E−18
GO:9205	purine ribonucleoside triphosphate metabolic process	1.23E−18
GO:32269	negative regulation of cellular protein metabolic process	1.31E−18
GO:48731	system development	1.36E−18
GO:9154	purine ribonucleotide catabolic process	1.40E−18
GO:32270	positive regulation of cellular protein metabolic process	1.43E−18
GO:9261	ribonucleotide catabolic process	1.45E−18
GO:43168	anion binding	1.48E−18
GO:9144	purine nucleoside triphosphate metabolic process	1.53E−18
GO:9199	ribonucleoside triphosphate metabolic process	1.59E−18
GO:34645	cellular macromolecule biosynthetic process	1.63E−18
GO:1901292	nucleoside phosphate catabolic process	1.64E−18
GO:51438	regulation of ubiquitin-protein ligase activity	1.67E−18
GO:44773	mitotic DNA damage checkpoint	2.06E−18
GO:31145	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic	2.06E−18
	process
GO:33554	cellular response to stress	2.39E−18
GO:2000045	regulation of G1/S transition of mitotic cell cycle	3.48E−18
GO:46434	organophosphate catabolic process	3.74E−18
GO:5654	nucleoplasm	3.91E−18
GO:51340	regulation of ligase activity	4.15E−18
GO:43202	lysosomal lumen	4.56E−18
GO:5975	carbohydrate metabolic process	4.84E−18
GO:9141	nucleoside triphosphate metabolic process	4.84E−18
GO:6986	response to unfolded protein	5.15E−18
GO:44774	mitotic DNA integrity checkpoint	5.47E−18
GO:35966	response to topologically incorrect protein	6.03E−18
GO:10565	regulation of cellular ketone metabolic process	6.03E−18
GO:31397	negative regulation of protein ubiquitination	6.34E−18
GO:48471	perinuclear region of cytoplasm	7.05E−18
GO:1901265	nucleoside phosphate binding	8.41E−18
GO:5775	vacuolar lumen	1.45E−17
GO:90	mitotic anaphase	1.55E−17
GO:15629	actin cytoskeleton	1.74E−17
GO:166	nucleotide binding	1.83E−17
GO:31398	positive regulation of protein ubiquitination	1.96E−17
GO:51322	anaphase	2.09E−17
GO:16491	oxidoreductase activity	2.23E−17
GO:9203	ribonucleoside triphosphate catabolic process	2.23E−17
GO:9207	purine ribonucleoside triphosphate catabolic process	2.23E−17
GO:9146	purine nucleoside triphosphate catabolic process	2.51E−17
GO:9143	nucleoside triphosphate catabolic process	3.05E−17
GO:45333	cellular respiration	3.05E−17
GO:5886	plasma membrane	3.46E−17
GO:87	M phase of mitotic cell cycle	4.52E−17
GO:44770	cell cycle phase transition	5.24E−17
GO:44772	mitotic cell cycle phase transition	5.24E−17
GO:51247	positive regulation of protein metabolic process	7.30E−17
GO:6458	‘de novo’ protein folding	9.71E−17
GO:7275	multicellular organismal development	1.04E−16
GO:502	proteasome complex	1.42E−16
GO:2000602	regulation of interphase of mitotic cell cycle	1.42E−16
GO:19866	organelle inner membrane	2.10E−16
GO:43161	proteasomal ubiquitin-dependent protein catabolic process	2.17E−16
GO:31966	mitochondrial membrane	2.20E−16
GO:51259	protein oligomerization	2.85E−16
GO:71156	regulation of cell cycle arrest	3.27E−16
GO:5740	mitochondrial envelope	3.37E−16
GO:10498	proteasomal protein catabolic process	3.39E−16
GO:44271	cellular nitrogen compound biosynthetic process	3.57E−16
GO:30168	platelet activation	3.67E−16
GO:90068	positive regulation of cell cycle process	4.24E−16
GO:279	M phase	5.00E−16
GO:1901362	organic cyclic compound biosynthetic process	6.09E−16
GO:5730	nucleolus	6.44E−16
GO:278	mitotic cell cycle	6.78E−16
GO:6006	glucose metabolic process	8.30E−16
GO:9892	negative regulation of metabolic process	8.50E−16
GO:34654	nucleobase-containing compound biosynthetic process	1.38E−15
GO:51082	unfolded protein binding	1.60E−15
GO:32879	regulation of localization	1.88E−15
GO:5576	extracellular region	1.92E−15
GO:22402	cell cycle process	2.13E−15
GO:904	cell morphogenesis involved in differentiation	2.28E−15
GO:44767	single-organism developmental process	2.78E−15
GO:280	nuclear division	3.26E−15
GO:7067	mitosis	3.26E−15
GO:42803	protein homodimerization activity	3.30E−15
GO:44724	single-organism carbohydrate catabolic process	3.96E−15
GO:44093	positive regulation of molecular function	4.06E−15
GO:209	protein polyubiquitination	4.93E−15
GO:16052	carbohydrate catabolic process	6.89E−15
GO:44723	single-organism carbohydrate metabolic process	7.22E−15
GO:5743	mitochondrial inner membrane	8.83E−15
GO:7049	cell cycle	9.62E−15
GO:51084	‘de novo’ posttranslational protein folding	1.02E−14
GO:71310	cellular response to organic substance	1.02E−14
GO:34622	cellular macromolecular complex assembly	1.23E−14
GO:84	S phase of mitotic cell cycle	1.27E−14
GO:44437	vacuolar part	1.27E−14
GO:5793	endoplasmic reticulum-Golgi intermediate compartment	1.32E−14
GO:19438	aromatic compound biosynthetic process	1.39E−14
GO:31396	regulation of protein ubiquitination	1.50E−14
GO:51320	S phase	1.73E−14
GO:31400	negative regulation of protein modification process	2.26E−14
GO:31324	negative regulation of cellular metabolic process	2.34E−14
GO:5773	vacuole	2.41E−14
GO:30659	cytoplasmic vesicle membrane	2.82E−14
GO:48285	organelle fission	3.14E−14
GO:31325	positive regulation of cellular metabolic process	3.16E−14
GO:5615	extracellular space	3.23E−14
GO:6520	cellular amino acid metabolic process	3.96E−14
GO:43167	ion binding	4.35E−14
GO:18130	heterocycle biosynthetic process	5.13E−14
GO:902	cell morphogenesis	6.14E−14
GO:51129	negative regulation of cellular component organization	6.16E−14
GO:31401	positive regulation of protein modification process	6.41E−14
GO:6396	RNA processing	7.17E−14
GO:82	G1/S transition of mitotic cell cycle	7.24E−14
GO:9893	positive regulation of metabolic process	9.47E−14
GO:5764	lysosome	1.27E−13
GO:6928	cellular component movement	1.45E−13
GO:8092	cytoskeletal protein binding	1.49E−13
GO:44259	multicellular organismal macromolecule metabolic process	1.52E−13
GO:8380	RNA splicing	1.52E−13
GO:12506	vesicle membrane	1.60E−13
GO:10605	negative regulation of macromolecule metabolic process	1.66E−13
GO:323	lytic vacuole	1.84E−13
GO:3729	mRNA binding	1.90E−13
GO:5996	monosaccharide metabolic process	2.03E−13
GO:46364	monosaccharide biosynthetic process	2.19E−13
GO:3779	actin binding	2.20E−13
GO:22617	extracellular matric disassembly	2.28E−13
GO:10035	response to inorganic substance	2.35E−13
GO:31012	extracellular matrix	3.04E−13
GO:377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	3.19E−13
GO:398	mRNA splicing, via spliceosome	3.19E−13
GO:1901991	negative regulation of mitotic cell cycle phase transition	3.52E−13
GO:32963	collagen metabolic process	3.85E−13
GO:7093	mitotic cell cycle checkpoint	3.86E−13
GO:42645	mitochondrial nucleoid	4.70E−13
GO:375	RNA splicing, via transesterification reactions	5.09E−13
GO:16616	oxidoreductase activity, acting on the CH—OH group of donors, NAD or NADP as acceptor	5.09E−13
GO:16614	oxidoreductase activity, acting on CH—OH group of donors	6.41E−13
GO:9295	nucleoid	6.79E−13
GO:65009	regulation of molecular function	6.89E−13
GO:44236	multicellular organismal metabolic process	6.89E−13
GO:51130	positive regulation of cellular component organization	7.06E−13
GO:19318	hexose metabolic process	7.22E−13
GO:5200	structural constituent of cytosketeton	8.23E−13
GO:16020	membrane	9.24E−13
GO:9653	anatomical structure morphogenesis	9.56E−13
GO:30574	collagen catabolic process	9.67E−13
GO:30029	actin filament-based process	1.05E−12
GO:42175	nuclear outer membrane-endoplasmic reticulum membrane network	1.38E−12
GO:43069	negative regulation of programmed cell death	1.49E−12
GO:1775	cell activation	1.75E−12
GO:43254	regulation of protein complex assembly	1.78E−12
GO:77	DNA damage checkpoint	1.92E−12
GO:50790	regulation of catalytic activity	2.00E−12
GO:46983	protein dimerization activity	2.02E−12
GO:32989	cellular component morphogenesis	2.17E−12
GO:17111	nucleoside-triphosphatase activity	2.21E−12
GO:42995	cell projection	2.48E−12
GO:46365	monosaccharide catabolic process	2.72E−12
GO:43085	positive regulation of catalytic activity	4.06E−12
GO:5681	spliceosomal complex	4.31E−12
GO:9986	cell surface	4.34E−12
GO:44087	regulation of cellular component biogenesis	4.70E−12
GO:31570	DNA integrity checkpoint	4.80E−12
GO:31399	regulation of protein modification process	5.08E−12
GO:44243	multicellular organismal catabolic process	5.08E−12
GO:16462	pyrophosphatase activity	5.29E−12
GO:51049	regulation of transport	5.76E−12
GO:3824	catalytic activity	6.58E−12
GO:22624	proteasome accessory complex	6.62E−12
GO:18279	protein N-linked glycosylation via asparagine	7.02E−12
GO:44420	extracellular matrix part	7.27E−12
GO:5578	proteinaceous extracellular matrix	7.34E−12
GO:32403	protein complex binding	7.34E−12
GO:1901990	regulation of mitotic cell cycle phase transition	7.37E−12
GO:7411	axon guidance	7.68E−12
GO:5201	extracellular matrix structural constituent	7.72E−12
GO:18196	peptidyl-asparagine modification	8.21E−12
GO:16818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	8.29E−12
GO:22613	ribonucleoprotein complex biogenesis	8.33E−12
GO:60548	negative regulation of cell death	9.66E−12
GO:32069	regulation of nuclease activity	9.69E−12
GO:19320	hexose catabolic process	1.12E−11
GO:43066	negative regulation of apoptotic process	1.26E−11
GO:6007	glucose catabolic process	1.43E−11
GO:16817	hydrolase activity, acting on acid anhydrides	1.44E−11
GO:5938	cell cortex	1.62E−11
GO:97159	organic cyclic compound binding	1.62E−11
GO:1901066	guanosine-containing compound metabolic process	1.71E−11
GO:9060	aerobic respiration	1.80E−11
GO:6575	cellular modified amino acid metabolic process	1.85E−11
GO:19886	antigen processing and presentation of exogenous peptide antigen via MHC class II	2.16E−11
GO:32787	monocarboxylic acid metabolic process	2.19E−11
GO:6397	mRNA processing	2.61E−11
GO:6099	tricarboxylic acid cycle	2.63E−11
GO:48199	vesicle targeting, to, from or within Golgi	2.68E−11
GO:6987	activation of signaling protein activity involved in unfolded protein response	2.69E−11
GO:48193	Golgi vesicle transport	2.72E−11
GO:6081	cellular aldehyde metabolic process	3.22E−11
GO:42743	hydrogen peroxide metabolic process	3.22E−11
GO:48858	cell projection morphogenesis	3.36E−11
GO:2495	antigen processing and presentation of peptide antigen via MHC class II	3.39E−11
GO:30030	cell projection organization	3.51E−11
GO:32774	RNA biosynthetic process	3.51E−11
GO:33043	regulation of organelle organization	3.65E−11
GO:32075	positive regulation of nuclease activity	4.14E−11
GO:30968	endoplasmic reticulum unfolded protein response	4.25E−11
GO:43603	cellular amide metabolic process	4.27E−11
GO:30154	cell differentiation	4.42E−11
GO:6984	ER-nucleus signaling pathway	4.53E−11
GO:71013	catalytic step 2 spliceosome	4.63E−11
GO:48667	cell morphogenesis involved in neuron differentiation	4.66E−11
GO:1901363	heterocyclic compound binding	4.87E−11
GO:16209	antioxidant activity	5.02E−11
GO:22403	cell cycle phase	5.24E−11
GO:32990	cell part morphogenesis	6.26E−11
GO:5789	endoplasmic reticulum membrane	6.26E−11
GO:5102	receptor binding	6.83E−11
GO:48666	neuron development	7.55E−11
GO:30036	actin cytoskeleton organization	9.08E−11
GO:15630	microtubule cytoskeleton	9.52E−11
GO:6487	protein N-linked glycosylation	1.07E−10
GO:1901700	response to oxygen-containing compound	1.20E−10
GO:72593	reactive oxygen species metabolic process	1.23E−10
GO:7010	cytoskeleton organization	1.23E−10
GO:34620	cellular response to unfolded protein	1.28E−10
GO:51920	peroxiredoxin activity	1.35E−10
GO:48468	cell development	1.42E−10
GO:46039	GTP metabolic process	1.45E−10
GO:6805	xenobiotic metabolic process	1.61E−10
GO:6518	peptide metabolic process	1.67E−10
GO:7409	axonogenesis	1.67E−10
GO:10604	positive regulation of macromolecule metabolic process	1.75E−10
GO:48583	regulation of response to stimulus	1.78E−10
GO:71466	cellular response to xenobiotic stimulus	1.78E−10
GO:48869	cellular developmental process	1.86E−10
GO:32386	regulation of intracellular transport	1.87E−10
GO:51329	interphase of mitotic cell cycle	1.87E−10
GO:44712	single-organism catabolic process	1.96E−10
GO:44282	small molecule catabolic process	1.96E−10
GO:6901	vesicle coating	2.16E−10
GO:22008	neurogenesis	2.20E−10
GO:31175	neuron projection development	2.29E−10
GO:51325	interphase	2.35E−10
GO:6793	phosphorus metabolic process	2.44E−10
GO:6732	coenzyme metabolic process	2.51E−10
GO:16787	hydrolase activity	2.72E−10
GO:9410	response to xenobiotic stimulus	3.05E−10
GO:1901069	guanosine-containing compound catabolic process	3.20E−10
GO:42592	homeostatic process	4.46E−10
GO:34614	cellular response to reactive oxygen species	4.84E−10
GO:6790	sulfur compound metabolic process	5.66E−10
GO:16051	carbohydrate biosynthetic process	5.99E−10
GO:6900	membrane budding	6.08E−10
GO:30016	myofibril	6.27E−10
GO:8064	regulation of actin polymerization or depolymerization	6.35E−10
GO:30832	regulation of actin filament length	7.03E−10
GO:32970	regulation of actin filament-based process	7.06E−10
GO:48699	generation of neurons	7.12E−10
GO:16050	vesicle organization	8.37E−10
GO:48812	neuron projection morphogenesis	8.77E−10
GO:6094	gluconeogenesis	8.91E−10
GO:35967	cellular response to topologically incorrect protein	8.99E−10
GO:19362	pyridine nucleotide metabolic process	8.99E−10
GO:46496	nicotinamide nucleotide metabolic process	8.99E−10
GO:1882	nucleoside binding	9.47E−10
GO:3924	GTPase activity	9.63E−10
GO:45087	innate immune response	1.07E−09
GO:6184	GTP catabolic process	1.25E−09
GO:17076	purine nucleotide binding	1.37E−09
GO:30863	cortical cytoskeleton	1.58E−09
GO:90066	regulation of anatomical structure size	1.60E−09
GO:6446	regulation of translational initiation	1.84E−09
GO:6629	lipid metabolic process	1.85E−09
GO:32555	purine ribonucleotide binding	2.00E−09
GO:43292	contractile fiber	2.05E−09
GO:32940	secretion by cell	2.16E−09
GO:5811	lipid particle	2.16E−09
GO:32550	purine ribonucleoside binding	2.21E−09
GO:19941	modification-dependent protein catabolic process	2.28E−09
GO:6913	nucleocytoplasmic transport	2.29E−09
GO:1883	purine nucleoside binding	2.29E−09
GO:6903	vesicle targeting	2.44E−09
GO:43632	modification-dependent macromolecule catabolic process	2.57E−09
GO:32549	ribonucleoside binding	2.76E−09
GO:32535	regulation of cellular component size	2.84E−09
GO:22900	electron transport chain	2.91E−09
GO:51169	nuclear transport	2.93E−09
GO:6749	glutathione metabolic process	3.01E−09
GO:7346	regulation of mitotic cell cycle	3.06E−09
GO:72524	pyridine-containing compound metabolic process	3.06E−09
GO:10564	regulation of cell cycle process	3.17E−09
GO:35639	purine ribonucleoside triphosphate binding	3.42E−09
GO:40011	locomotion	3.54E−09
GO:42274	ribosomal small subunit biogenesis	3.54E−09
GO:32553	ribonucleotide binding	3.56E−09
GO:6887	exocytosis	3.65E−09
GO:51168	cofactor metabolic process	3.74E−09
GO:9719	response to endogenous stimulus	3.89E−09
GO:23051	regulation of signaling	4.35E−09
GO:52547	regulation of peptidase activity	4.56E−09
GO:6112	energy reserve metabolic process	4.56E−09
GO:42744	hydrogen peroxide catabolic process	4.60E−09
GO:2504	antigen processing and presentation of peptide or polysaccharide antigen via MHC class II	4.67E−09
GO:1901988	negative regulation of cell cycle phase transition	4.68E−09
GO:46903	secretion	4.73E−09
GO:6508	proteolysis	4.83E−09
GO:6511	ubiquitin-dependent protein catabolic process	5.14E−09
GO:30833	regulation of actin filament polymerization	5.14E−09
GO:35964	COPI-coated vesicle budding	5.17E−09
GO:48205	COPI coating of Golgi vesicle	5.17E−09
GO:48200	Golgi transport vesicle coating	5.17E−09
GO:5178	integrin binding	5.33E−09
GO:34599	cellular response to oxidative stress	6.28E−09
GO:6979	response to oxidative stress	6.28E−09
GO:70301	cellular response to hydrogen peroxide	6.65E−09
GO:46034	ATP metabolic process	6.86E−09
GO:6796	phosphate-containing compound metabolic process	7.41E−09
GO:30834	regulation of actin filament depolymerization	7.55E−09
GO:44257	cellular protein catabolic process	7.83E−09
GO:75	cell cycle checkpoint	7.96E−09
GO:42254	ribosome biogenesis	8.31E−09
GO:10646	regulation of cell communication	8.45E−09
GO:30139	endocytic vesicle	8.46E−09
GO:50662	coenzyme binding	8.94E−09
GO:22904	respiratory electron transport chain	9.17E−09
GO:30182	neuron differentiation	9.24E−09
GO:30017	sarcomere	9.24E−09
GO:44700	single organism signaling	9.45E−09
GO:23052	signaling	9.45E−09
GO:7154	cell communication	9.75E−09
GO:48194	Golgi vesicle budding	9.85E−09
GO:34976	response to endoplasmic reticulum stress	1.01E−08
GO:32271	regulation of protein polymerization	1.01E−08
GO:44449	contractile fiber part	1.03E−08
GO:1901701	cellular response to oxygen-containing compound	1.12E−08
GO:5635	nuclear envelope	1.16E−08
GO:19319	hexose biosynthetic process	1.22E−08
GO:48513	organ development	1.25E−08
GO:1901987	regulation of cell cycle phase transition	1.32E−08
GO:51603	proteolysis involved in cellular protein catabolic process	1.33E−08
GO:7399	nervous system development	1.42E−08
GO:16054	organic acid catabolic process	1.42E−08
GO:46395	carboxylic acid catabolic process	1.42E−08
GO:6200	ATP catabolic process	1.43E−08
GO:31252	cell leading edge	1.43E−08
GO:6733	oxidoreduction coenzyme metabolic process	1.52E−08
GO:19222	regulation of metabolic process	1.75E−08
GO:9605	response to external stimulus	1.91E−08
GO:10638	positive regulation of organelle organization	1.95E−08
GO:51650	establishment of vesicle localization	2.15E−08
GO:30199	collagen fibril organization	2.15E−08
GO:42542	response to hydrogen peroxide	2.28E−08
GO:48037	cofactor binding	2.29E−08
GO:7044	cell-substrate junction assembly	2.40E−08
GO:44273	sulfur compound catabolic process	2.48E−08
GO:5604	basement membrane	2.51E−08
GO:16684	oxidoreductase activity, acting on peroxide as acceptor	2.51E−08
GO:4601	peroxidase activity	2.51E−08
GO:1901137	carbohydrate derivative biosynthetic process	2.83E−08
GO:30496	midbody	2.99E−08
GO:30155	regulation of cell adhesion	2.99E−08
GO:32956	regulation of actin cytoskeleton organization	3.05E−08
GO:42127	regulation of cell proliferation	3.24E−08
GO:51270	regulation of cellular component movement	3.31E−08
GO:6952	defense response	3.56E−08
GO:6897	endocytosis	3.59E−08
GO:3988	acetyl-CoA C-acyltransferase activity	3.65E−08
GO:7165	signal transduction	3.69E−08
GO:30163	protein catabolic process	3.70E−08
GO:51336	regulation of hydrolase activity	3.96E−08
GO:16528	sarcoplasm	3.97E−08
GO:16667	oxidoreductase activity, acting on a sulfur group of donors	4.02E−08
GO:51693	actin filament capping	4.08E−08
GO:302	response to reactive oxygen species	4.13E−08
GO:22618	ribonucleoprotein complex assembly	4.64E−08
GO:51493	regulation of cytoskeleton organization	4.80E−08
GO:42330	taxis	4.80E−08
GO:6935	chemotaxis	4.80E−08
GO:42641	actomyosin	4.84E−08
GO:6096	glycolysis	5.05E−08
GO:60255	regulation of macromolecule metabolic process	5.40E−08
GO:6739	NADP metabolic process	5.75E−08
GO:1725	stress fiber	5.77E−08
GO:19725	cellular homeostasis	5.78E−08
GO:51726	regulation of cell cycle	6.18E−08
GO:34329	cell junction assembly	6.71E−08
GO:1525	angiogenesis	6.72E−08
GO:6974	response to DNA damage stimulus	7.29E−08
GO:22603	regulation of anatomical structure morphogenesis	7.40E−08
GO:10948	negative regulation of cell cycle process	7.42E−08
GO:5832	chaperonin-containing T-complex	7.84E−08
GO:19901	protein kinase binding	8.03E−08
GO:31406	carboxylic acid binding	8.03E−08
GO:6921	cellular component disassembly involved in execution phase of apoptosis	8.10E−08
GO:71826	ribonucleoprotein complex subunit organization	8.21E−08
GO:19900	kinase binding	8.36E−08
GO:48029	monosaccharide binding	8.78E−08
GO:5794	Golgi apparatus	9.87E−08
GO:51239	regulation of multicellular organismal process	9.88E−08
GO:50661	NADP binding	9.88E−08
GO:30120	vesicle coat	9.88E−08
GO:60341	regulators of cellular localization	9.95E−08
GO:31333	negative regulation of protein complex assembly	1.06E−07
GO:30662	coated vesicle membrane	1.22E−07
GO:5080	protein kinase C binding	1.25E−07
GO:9888	tissue development	1.27E−07
GO:2001233	regulation of apoptotic signaling pathway	1.38E−07
GO:97194	execution phase of apoptosis	1.39E−07
GO:5583	fibrillar collagen	1.47E−07
GO:51235	maintenance of location	1.54E−07
GO:48584	positive regulation of response to stimulus	1.63E−07
GO:51656	establishment of organelle localization	1.66E−07
GO:42383	sarcolemma	1.78E−07
GO:30141	secretory granule	1.78E−07
GO:34330	cell junction organization	1.89E−07
GO:30835	negative regulation of actin filament depolymerization	1.92E−07
GO:52548	regulation of endopeptidase activity	2.21E−07
GO:1726	ruffle	2.27E−07
GO:50793	regulation of developmental process	2.33E−07
GO:15036	disulfide oxidoreductase activity	2.33E−07
GO:80134	regulation of response to stress	2.37E−07
GO:51287	NAD binding	2.50E−07
GO:32432	actin filament bundle	2.54E−07
GO:42026	protein refolding	2.63E−07
GO:48585	negative regulation of response to stimulus	2.63E−07
GO:44448	cell cortex part	2.93E−07
GO:5516	calmodulin binding	2.93E−07
GO:9055	electron carrier activity	2.93E−07
GO:34660	ncRNA metabolic process	3.19E−07
GO:51640	organelle localization	3.40E−07
GO:31589	cell-substrate adhesion	3.47E−07
GO:7015	actin filament organization	3.58E−07
GO:16864	intramolecular oxidoreductase activity, transposing S—S bonds	3.73E−07
GO:3756	protein disulfide isomerase activity	3.73E−07
GO:9966	regulation of signal transduction	3.76E−07
GO:23057	negative regulation of signaling	4.15E−07
GO:48407	platelet-derived growth factor binding	4.45E−07
GO:44262	cellular carbohydrate metabolic process	4.45E−07
GO:43687	post-translational protein modification	4.60E−07
GO:35556	intracellular signal transduction	4.65E−07
GO:10038	response to metal ion	5.16E−07
GO:60314	regulation of ryanodine-sensitive calcium-release channel activity	5.22E−07
GO:16862	intramolecular oxidoreductase activity, interconverting keto- and enol-groups	5.22E−07
GO:30490	maturation of SSU-rRNA	5.41E−07
GO:9628	response to abiotic stimulus	5.59E−07
GO:51087	chaperone binding	5.68E−07
GO:10952	positive regulation of peptidase activity	5.74E−07
GO:30137	COPI-coated vesicle	6.86E−07
GO:6740	NADPH regeneration	6.86E−07
GO:16887	ATPase activity	6.95E−07
GO:51260	protein homooligomerization	7.13E−07
GO:18193	peptidyl-amino acid modification	7.13E−07
GO:30126	COPI vesicle coat	7.16E−07
GO:34446	substrate adhesion-dependent cell spreading	8.63E−07
GO:5525	GTP binding	8.65E−07
GO:80090	regulation of primary metabolic process	8.86E−07
GO:6027	glycosaminoglycan catabolic process	8.91E−07
GO:97458	neuron part	9.20E−07
GO:16860	intramolecular oxidoreductase activity	9.23E−07
GO:9968	negative regulation of signal transduction	9.28E−07
GO:1568	blood vessel development	9.58E−07
GO:30663	COPI-coated vesicle membrane	9.68E−07
GO:5509	calcium ion binding	1.16E−06
GO:5874	microtubule	1.17E−06
GO:10648	negative regulation of cell communication	1.19E−06
GO:10942	positive regulation of cell death	1.21E−06
GO:46930	pore complex	1.35E−06
GO:51291	protein heterooligomerization	1.35E−06
GO:32561	guanyl ribonucleotide binding	1.38E−06
GO:1901698	response to nitrogen compound	1.39E−06
GO:30334	regulation of cell migration	1.48E−06
GO:44255	cellular lipid metabolic process	1.57E−06
GO:43122	regulation of I-kappaB kinase/NF-kappaB cascade	1.57E−06
GO:31347	regulation of defense response	1.59E−06
GO:19001	guanyl nucleotide binding	1.64E−06
GO:16408	C-acyltransferase activity	1.66E−06
GO:33119	negative regulation of RNA splicing	1.66E−06
GO:5581	collagen	1.73E−06
GO:60205	cytoplasmic membrane-bounded vesicle lumen	1.78E−06
GO:6909	phagocytosis	1.81E−06
GO:7160	cell-matrix adhesion	1.86E−06
GO:8022	protein C-terminus binding	1.86E−06
GO:51262	protein tetramerization	1.89E−06
GO:31983	vesicle lumen	2.03E−06
GO:31323	regulation of cellular metabolic process	2.08E−06
GO:42255	ribosome assembly	2.21E−06
GO:32154	cleavage furrow	2.42E−06
GO:15949	nucleobase-containing small molecule interconversion	2.42E−06
GO:30135	coated vesicle	2.54E−06
GO:10243	response to organic nitrogen	2.61E−06
GO:44711	single-organism biosynthetic process	2.79E−06
GO:3697	single-stranded DNA binding	2.79E−06
GO:72359	circulatory system development	2.80E−06
GO:72358	cardiovascular system development	2.80E−06
GO:32272	negative regulation of protein polymerization	2.93E−06
GO:30554	adenyl nucleotide binding	3.00E−06
GO:8544	epidermis development	3.03E−06
GO:6955	immune response	3.14E−06
GO:51168	nuclear export	3.14E−06
GO:1944	vasculature development	3.18E−06
GO:51236	establisment of RNA localization	3.39E−06
GO:50657	nucleic acid transport	3.39E−06
GO:50658	RNA transport	3.39E−06
GO:17148	negative regulation of translation	3.43E−06
GO:2000145	regulation of cell motility	3.48E−06
GO:30837	negative regulation of actin filament polymerization	3.49E−06
GO:1901028	regulation of mitochondrial outer membrane permeabilization	3.60E−06
GO:6364	rRNA processing	3.68E−06
GO:22610	biological adhesion	3.69E−06
GO:43005	neuron projection	3.85E−06
GO:30660	Golgi-associated vesicle membrane	3.89E−06
GO:22604	regulation of cell morphogenesis	3.94E−06
GO:44283	small molecule biosynthetic process	4.02E−06
GO:44459	plasma membrane part	4.04E−06
GO:51050	positive regulation of transport	4.15E−06
GO:72522	purine-containing compound biosynthetic process	4.17E−06
GO:5798	Golgi-associated vesicle	4.18E−06
GO:51289	protein homotetramerization	4.22E−06
GO:16567	protein ubiquitination	4.25E−06
GO:45859	regulation of protein kinase activity	4.28E−06
GO:30666	endocytic vesicle membrane	4.62E−06
GO:32559	adenyl ribonucleotide binding	4.67E−06
GO:51648	vesicle localization	4.71E−06
GO:19843	rRNA binding	4.92E−06
GO:4857	enzyme inhibitor activity	5.22E−06
GO:10821	regulation of mitochondrion organization	5.33E−06
GO:43244	regulation of protein complex disassembly	5.65E−06
GO:40012	regulation of locomotion	5.65E−06
GO:6403	RNA localization	5.96E−06
GO:16651	oxidoreductase activity, acting on NAD(P)H	5.96E−06
GO:30512	negative regulation of transforming growth factor beta receptor signaling pathway	5.96E−06
GO:6026	aminoglycan catabolic process	5.96E−06
GO:9100	glycoprotein metabolic process	6.14E−06
GO:5518	collagen binding	6.20E−06
GO:42340	keratan sulfate catabolic process	6.42E−06
GO:8143	poly(A) RNA binding	6.42E−06
GO:2682	regulation of immune system process	6.54E−06
GO:32153	cell division site	6.85E−06
GO:32155	cell division site part	6.85E−06
GO:8238	exopeptidase activity	6.90E−06
GO:48514	blood vessel morphogenesis	7.01E−06
GO:70647	protein modification by small protein conjugation or removal	7.09E−06
GO:43549	regulation of kinase activity	7.11E−06
GO:16072	rRNA metabolic process	7.30E−06
GO:61134	peptidase regulator activity	7.39E−06
GO:5905	coated pit	7.39E−06
GO:16053	organic acid biosynthetic process	7.93E−06
GO:46394	carboxylic acid biosynthetic process	7.93E−06
GO:7155	cell adhesion	7.99E−06
GO:31093	platelet alpha granule lumen	7.99E−06
GO:1901879	regulation of protein depolymerization	8.23E−06
GO:6098	pentose-phosphate shunt	8.47E−06
GO:6890	retrograde vesicle-mediated transport, Golgi to ER	8.47E−06
GO:10822	positive regulation of mitochondrion organization	9.36E−06
GO:4364	glutathione transferase activity	9.36E−06
GO:10627	regulation of intracellular protein kinase cascade	9.58E−06
GO:15035	protein disulfide oxidoreductase activity	9.78E−06
GO:43206	extracellular fibril organization	9.99E−06
GO:97435	fibril organization	9.99E−06
GO:48025	negative regulation of mRNA splicing, via spliceosome	9.99E−06
GO:43065	positive regulation of apoptotic process	1.01E−05
GO:6892	post-Golgi vesicle-mediated transport	1.01E−05
GO:5782	peroxisomal matrix	1.03E−05
GO:31907	microbody lumen	1.03E−05
GO:6937	regulation of muscle contraction	1.04E−05
GO:44242	cellular lipid catabolic process	1.07E−05
GO:19003	GDP binding	1.08E−05
GO:6144	purine nucleobase metabolic process	1.08E−05
GO:16529	sarcoplasmic reticulum	1.10E−05
GO:9112	nucleobase metabolic process	1.12E−05
GO:70848	response to growth factor stimulus	1.19E−05
GO:43068	positive regulation of programmed cell death	1.32E−05
GO:51338	regulation of transferase activity	1.34E−05
GO:16835	carbon-oxygen lyase activity	1.36E−05
GO:19838	growth factor binding	1.38E−05
GO:5524	ATP binding	1.41E−05
GO:10880	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum	1.44E−05
GO:1900739	regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling	1.44E−05
	pathway
GO:1900740	positive regulation of protein insertion into mitochondrial membrane involved in apoptotic	1.44E−05
	signaling pathway
GO:9165	nucleotide biosynthetic process	1.44E−05
GO:30864	cortical actin cytoskeleton	1.45E−05
GO:42451	purine nucleoside biosynthetic process	1.47E−05
GO:46129	purine ribonucleoside biosynthetic process	1.47E−05
GO:2000116	regulation of cysteine-type endopeptidase activity	1.50E−05
GO:32446	protein modification by small protein conjugation	1.50E−05
GO:15931	nucleobase-containing compound transport	1.52E−05
GO:14070	response to organic cyclic compound	1.54E−05
GO:55117	regulation of cardiac muscle contraction	1.56E−05
GO:70925	organelle assembly	1.57E−05
GO:90101	negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway	1.64E−05
GO:28	ribosomal small subunit assembly	1.71E−05
GO:462	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	1.71E−05
GO:4032	alditol: NADP + 1-oxidoreductase activity	1.71E−05
GO:19322	pentose biosynthetic process	1.71E−05
GO:51345	positive regulation of hydrolase activity	1.72E−05
GO:8284	positive regulation of cell proliferation	1.80E−05
GO:5885	Arp2/3 protein complex	1.83E−05
GO:45785	positive regulation of cell adhesion	1.90E−05
GO:60627	regulation of vesicle-mediated transport	1.97E−05
GO:6164	purine nucleotide biosynthetic process	2.03E−05
GO:32880	regulation of protein localization	2.14E−05
GO:9068	aspartate family amino acid catabolic process	2.15E−05
GO:70717	poly-purine tract binding	2.15E−05
GO:50686	negative regulation of mRNA processing	2.15E−05
GO:70482	response to oxygen levels	2.19E−05
GO:71103	DNA conformation change	2.28E−05
GO:1901293	nucleoside phosphate biosynthetic process	2.30E−05
GO:70993	translation preinitiation complex	2.31E−05
GO:33290	eukaryotic 48S preinitiation complex	2.31E−05
GO:16282	eukaryotic 43S preinitiation complex	2.31E−05
GO:31968	organelle outer membrane	2.49E−05
GO:8135	translation factor activity, nucleic acid binding	2.49E−05
GO:1901019	regulation of calcium ion transmembrane transporter activity	2.51E−05
GO:5766	endosome	2.58E−05
GO:6416	tRNA aminoacylation for protein translation	2.64E−05
GO:43648	dicarboxylic acid metabolic process	2.68E−05
GO:90288	negative regulation of cellular response to growth factor stimulus	2.68E−05
GO:9152	purine ribonucleotide biosynthetic process	2.77E−05
GO:43623	cellular protein complex assembly	2.83E−05
GO:46390	ribose phosphate biosynthetic process	2.87E−05
GO:35337	fatty-acyl-CoA metabolic process	2.90E−05
GO:1731	formation of translation preinitiation complex	2.90E−05
GO:8379	thioredoxin peroxidase activity	3.01E−05
GO:35583	sequestering of TGFbeta in extracellular matrix	3.01E−05
GO:19682	glyceraldehyde-3-phosphate metabolic process	3.01E−05
GO:42776	mitochondrial ATP synthesis coupled proton transport	3.01E−05
GO:3012	muscle system process	3.07E−05
GO:60249	anatomical structure homeostasis	3.15E−05
GO:43281	regulation of cysteine-type endopeptidase activity involved in apoptotic process	3.22E−05
GO:44548	S100 protein binding	3.25E−05
GO:45727	positive regulation of translation	3.33E−05
GO:51592	response to calcium ion	3.37E−05
GO:19904	protein domain specific binding	3.37E−05
GO:32388	positive regulation of intracellular transport	3.37E−05
GO:55072	iron ion homeostasis	3.66E−05
GO:30100	regulation of endocytosis	3.70E−05
GO:34504	protein localization to nucleus	3.91E−05
GO:31091	platelet alpha granule	4.11E−05
GO:35338	long-chain fatty-acyl-CoA biosynthetic process	4.15E−05
GO:72350	tricarboxylic acid metabolic process	4.15E−05
GO:1901566	organonitrogen compound biosynthetic process	4.15E−05
GO:51170	nuclear import	4.18E−05
GO:5815	microtubule organizing center	4.29E−05
GO:43039	tRNA aminoacylation	4.29E−05
GO:43038	amino acid activation	4.29E−05
GO:16197	endosomal transport	4.29E−05
GO:2001242	regulation of intrinsic apoptotic signaling pathway	4.39E−05
GO:71363	cellular response to growth factor stimulus	4.39E−05
GO:4177	aminopeptidase activity	4.49E−05
GO:3727	single-stranded RNA binding	4.61E−05
GO:51301	cell division	4.94E−05
GO:16853	isomerase activity	4.96E−05
GO:6942	regulation of striated muscle contraction	4.96E−05
GO:3008	system process	4.96E−05
GO:34774	secretory granule lumen	5.19E−05
GO:55082	cellular chemical homeostasis	5.23E−05
GO:72329	monocarboxylic acid catabolic process	5.27E−05
GO:10562	positive regulation of phosphorus metabolic process	5.46E−05
GO:45937	positive regulation of phosphate metabolic process	5.46E−05
GO:1666	response to hypoxia	5.46E−05
GO:19798	procollagen-proline dioxygenase activity	5.54E−05
GO:60316	positive regulation of ryanodine-sensitive calcium-release channel activity	5.56E−05
GO:6554	lysine catabolic process	5.56E−05
GO:6553	lysine metabolic process	5.56E−05
GO:44438	microbody part	5.69E−05
GO:44439	peroxisomal part	5.69E−05
GO:90257	regulation of muscle system process	5.72E−05
GO:5643	nuclear pore	5.72E−05
GO:5813	centrosome	5.72E−05
GO:35383	thioester metabolic process	5.96E−05
GO:6637	acyl-CoA metabolic process	5.96E−05
GO:17015	regulation of transforming growth factor beta receptor signaling pathway	6.01E−05
GO:19867	outer membrane	6.10E−05
GO:1901185	negative regulation of ERBB signaling pathway	6.19E−05
GO:42059	negative regulation of epidermal growth factor receptor signaling pathway	6.19E−05
GO:42579	microbody	6.23E−05
GO:5777	peroxisome	6.23E−05
GO:36293	response to decreased oxygen levels	6.28E−05
GO:44431	Golgi apparatus part	6.34E−05
GO:35384	thioester biosynthetic process	6.39E−05
GO:71616	acyl-CoA biosynthetic process	6.39E−05
GO:43021	ribonucleoprotein complex binding	6.50E−05
GO:6611	protein export from nucleus	7.10E−05
GO:2001257	regulation of cation channel activity	7.10E−05
GO:43123	positive regulation of I-kappaB kinase/NF-kappaB cascade	7.13E−05
GO:30133	transport vesicle	7.36E−05
GO:6334	nucleosome assembly	7.54E−05
GO:34399	nuclear periphery	7.92E−05
GO:51271	negative regulation of cellular component movement	7.98E−05
GO:50840	extracellular matrix binding	8.01E−05
GO:51028	mRNA transport	8.47E−05
GO:90287	regulation of cellular response to growth factor stimulus	8.47E−05
GO:70402	NADPH binding	8.84E−05
GO:60315	negative regulation of ryanodine-sensitive calcium-release channel activity	8.84E−05
GO:4029	aldehyde dehydrogenase (NAD) activity	8.84E−05
GO:16042	lipid catabolic process	8.84E−05
GO:7052	mitotic spindle organization	9.18E−05
GO:31941	filamentous actin	9.18E−05
GO:9260	ribonucleotide biosynthetic process	9.27E−05
GO:10881	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion	9.32E−05
GO:90114	COPII-coated vesicle budding	9.32E−05
GO:48208	COPII vesicle coating	9.32E−05
GO:48207	vesicle targeting, rough ER to cis-Golgi	9.32E−05
GO:46949	fatty-acyl-CoA biosynthetic process	9.32E−05
GO:42325	regulation of phosphorylation	9.46E−05
GO:10811	positive regulation of cell-substrate adhesion	9.72E−05
GO:70201	regulation of establishment of protein localization	9.72E−05
GO:51051	negative regulation of transport	9.81E−05
GO:1901880	negative regulation of protein depolymerization	9.83E−05
GO:33674	positive regulation of kinase activity	9.87E−05
GO:50900	leukocyte migration	1.01E−04
GO:2001056	positive regulation of cysteine-type endopeptidase activity	1.02E−04
GO:16477	cell migration	1.03E−04
GO:17038	protein import	1.03E−04
GO:9051	pentose-phosphate shunt, oxidative branch	1.08E−04
GO:43259	laminin-10 complex	1.08E−04
GO:3985	acetly-CoA C-acetyltransferase activity	1.08E−04
GO:30388	fructose 1,6-biphosphate metabolic process	1.08E−04
GO:42455	ribonucleoside biosynthetic process	1.09E−04
GO:31334	positive regulation of protein complex assembly	1.09E−04
GO:45454	cell redox homeostasis	1.10E−04
GO:6450	regulation of translational fidelity	1.18E−04
GO:31543	peptidyl-proline dioxygenase activity	1.18E−04
GO:55080	cation homeostasis	1.19E−04
GO:48306	calcium-dependent protein binding	1.20E−04
GO:45860	positive regulation of protein kinase activity	1.22E−04
GO:30246	carbohydrate binding	1.25E−04
GO:33764	steroid dehydrogenase activity, acting on the CH—OH group of donors, NAD or NADP as acceptor	1.27E−04
GO:51347	positive regulation of transferase activity	1.31E−04
GO:70208	protein heterotrimerization	1.32E−04
GO:51238	sequestering of metal ion	1.32E−04
GO:1934	positive regulation of protein phosphorylation	1.32E−04
GO:43242	negative regulation of protein complex disassembly	1.32E−04
GO:10522	regulation of calcium ion transport into cytosol	1.32E−04
GO:9066	aspartate family amino acid metabolic process	1.33E−04
GO:33143	regulation of intracellular steroid hormone receptor signaling pathway	1.33E−04
GO:44440	endosomal part	1.35E−04
GO:4812	aminoacyl-tRNA ligase activity	1.37E−04
GO:2000026	regulation of multicellular organismal development	1.39E−04
GO:1932	regulation of protein phosphorylation	1.43E−04
GO:35336	long-chain fatty-acyl-CoA metabolic process	1.48E−04
GO:51279	regulation of release of sequestered calcium ion into cytosol	1.51E−04
GO:3746	translation elongation factor activity	1.51E−04
GO:16875	ligase activity, forming carbon-oxygen bonds	1.54E−04
GO:16876	ligase activity, forming aminoacyl-tRNA and related compounds	1.54E−04
GO:31497	chromatin assembly	1.55E−04
GO:10950	positive regulation of endopeptidase activity	1.58E−04
GO:32993	protein-DNA complex	1.58E−04
GO:10951	negative regulation of endopeptidase activity	1.65E−04
GO:71495	cellular response to endogenous stimulus	1.68E−04
GO:44744	protein targeting to nucleus	1.68E−04
GO:6606	protein import into nucleus	1.68E−04
GO:44427	chromosomal part	1.70E−04
GO:9163	nucleoside biosynthetic process	1.71E−04
GO:1901659	glycosyl compound biosynthetic process	1.71E−04
GO:6448	regulation of translational elongation	1.72E−04
GO:30003	cellular cation homeostasis	1.75E−04
GO:90092	regulation of transmembrane receptor protein serine/threonine kinase signaling pathway	1.78E−04
GO:10810	regulation of cell-substrate adhesion	1.79E−04
GO:42176	regulation of protein catabolic process	1.83E−04
GO:6936	muscle contraction	1.83E−04
GO:75733	intracellular transport of viral material	1.83E−04
GO:910	cytokinesis	1.89E−04
GO:1900026	positive regulation of substrate adhesion-dependent cell spreading	1.90E−04
GO:9063	cellular amino acid catabolic process	1.90E−04
GO:50764	regulation of phagocytosis	1.92E−04
GO:6641	triglyceride metabolic process	1.94E−04
GO:48878	chemical homeostasis	1.94E−04
GO:16229	steroid dehydrogenase activity	1.99E−04
GO:6839	mitochondrial transport	2.00E−04
GO:2252	immune effector process	2.01E−04
GO:5544	calcium-dependent phospholipid binding	2.07E−04
GO:42327	positive regulation of phosphorylation	2.08E−04
GO:9167	purine ribonucleoside monophosphate metabolic process	2.10E−04
GO:6635	fatty acid beta-oxidation	2.10E−04
GO:31253	cell projection membrane	2.11E−04
GO:44463	cell projection part	2.15E−04
GO:19321	pentose metabolic process	2.25E−04
GO:5844	polysome	2.30E−04
GO:9126	purine nucleoside monophosphate metabolic process	2.35E−04
GO:30054	cell junction	2.37E−04
GO:34728	nucleosome organization	2.39E−04
GO:16836	hydro-lyase activity	2.41E−04
GO:30162	regulation of proteolysis	2.41E−04
GO:30117	membrane coat	2.42E−04
GO:48475	coated membrane	2.42E−04
GO:16453	C-acetyltransferase activity	2.47E−04
GO:70934	CRD-mediated mRNA stabilization	2.47E−04
GO:10649	regulation of cell communication by electrical coupling	2.47E−04
GO:71692	protein localization to extracellular region	2.47E−04
GO:71694	maintenance of protein location in extracellular region	2.47E−04
GO:48027	mRNA 5′-UTR binding	2.47E−04
GO:2000425	regulation of apoptotic cell clearance	2.47E−04
GO:3724	RNA helicase activity	2.62E−04
GO:19371	cyclooxygenase pathway	2.62E−04
GO:42178	xenobiotic catabolic process	2.62E−04
GO:30207	chondroitin sulfate catabolic process	2.62E−04
GO:5606	laminin-1 complex	2.62E−04
GO:10466	negative regulation of peptidase activity	2.62E−04
GO:8016	regulation of heart contraction	2.70E−04
GO:6405	RNA export from nucleus	2.70E−04
GO:30838	positive regulation of actin filament polymerization	2.70E−04
GO:10882	regulation of cardiac muscle contraction by calcium ion signaling	2.71E−04
GO:5753	mitochondrial proton-transporting ATP synthase complex	2.71E−04
GO:42623	ATPase activity, coupled	2.71E−04
GO:43280	positive regulation of cysteine-type endopeptidase activity involved in apoptotic process	2.83E−04
GO:9967	positive regulation of signal transduction	2.83E−04
GO:1101	response to acid	2.83E−04
GO:18149	peptide cross-linking	2.87E−04
GO:90407	organophosphate biosynthetic process	2.88E−04
GO:51651	maintenance of location in cell	2.94E−04
GO:9206	purine ribonucleoside triphosphate biosynthetic process	2.96E−04
GO:16765	transferase activity, transferring alkyl or aryl (other than methyl) groups	3.00E−04
GO:6639	acylglycerol metabolic process	3.00E−04
GO:9145	purine nucleoside triphosphate biosynthetic process	3.18E−04
GO:6873	cellular ion homeostasis	3.21E−04
GO:6638	neutral lipid metabolic process	3.42E−04
GO:9062	fatty acid catabolic process	3.49E−04
GO:71451	cellular response to superoxide	3.53E−04
GO:32964	collagen biosynthetic process	3.53E−04
GO:19430	removal of superoxide radicals	3.53E−04
GO:3857	3-hydroxyacyl-CoA dehydrogenase activity	3.53E−04
GO:8106	alcohol dehydrogenase (NADP+) activity	3.53E−04
GO:30934	anchoring collagen	3.53E−04
GO:5694	chromosome	3.65E−04
GO:6879	cellular iron ion homeostasis	3.66E−04
GO:45185	maintenance of protein location	3.66E−04
GO:86	G2/M transition of mitotic cell cycle	3.85E−04
GO:16504	peptidase activator activity	3.87E−04
GO:6754	ATP biosynthetic process	3.89E−04
GO:33118	endoplasmic reticulum-Golgi intermediate compartment membrane	3.90E−04
GO:19773	proteasome core complex, alpha-subunit complex	3.90E−04
GO:5741	mitochondrial outer membrance	3.99E−04
GO:9101	glycoprotein biosynthetic process	4.15E−04
GO:48646	anatomical structure formation involved in morphogenesis	4.26E−04
GO:97367	carbohydrate derivative binding	4.28E−04
GO:6399	tRNA metabolic process	4.34E−04
GO:6662	glycerol ether metabolic process	4.39E−04
GO:6333	chromatin assembly or disassembly	4.43E−04
GO:23056	positive regulation of signaling	4.48E−04
GO:22898	regulation of transmembrane transporter activity	4.49E−04
GO:51495	positive regulation of cytoskeleton organization	4.52E−04
GO:34470	ncRNA processing	4.63E−04
GO:50690	regulation of defense response to virus by virus	4.63E−04
GO:35581	sequestering of extracellular ligand from receptor	4.63E−04
GO:71437	invadopodium	4.63E−04
GO:16508	long-chain-enoyl-CoA hydratase activity	4.63E−04
GO:70937	CRD-mediated mRNA stability complex	4.63E−04
GO:30235	nitric-oxide synthase regulator activity	4.63E−04
GO:31581	hemidesmosome assembly	4.63E−04
GO:2001258	negative regulation of cation channel activity	4.63E−04
GO:46185	aldehyde catabolic process	4.63E−04
GO:70003	threonine-type peptidase activity	4.68E−04
GO:4298	threonine-type endopeptidase activity	4.68E−04
GO:10647	positive regulation of cell communication	4.73E−04
GO:9201	ribonucleoside triphosphate biosynthetic process	4.75E−04
GO:6888	ER to Golgi vesicle-mediated transport	4.75E−04
GO:139	Golgi membrane	4.84E−04
GO:6631	fatty acid metabolic process	4.86E−04
GO:32411	positive regulation of transporter activity	4.89E−04
GO:5774	vacuolar membrane	4.94E−04
GO:8283	cell proliferation	4.94E−04
GO:65004	protein-DNA complex assembly	4.97E−04
GO:5819	spindle	5.04E−04
GO:55037	recycling endosome	5.10E−04
GO:51272	positive regulation of cellular component movement	5.20E−04
GO:51674	localization of cell	5.21E−04
GO:50801	ion homeostasis	5.21E−04
GO:48870	cell motility	5.21E−04
GO:30203	glycosaminoglycan metabolic process	5.21E−04
GO:97190	apoptotic signaling pathway	5.24E−04
GO:43200	response to amino acid stimulus	5.37E−04
GO:43531	ADP binding	5.38E−04
GO:33144	negative regulation of intracellular steroid hormone receptor signaling pathway	5.38E−04
GO:9897	external side of plasma membrane	5.43E−04
GO:97193	intrinsic apoptotic signaling pathway	5.46E−04
GO:1900117	regulation of execution phase of apoptosis	5.63E−04
GO:30658	transport vesicle membrane	5.75E−04
GO:8290	F-actin capping protein complex	5.93E−04
GO:8250	oligosaccharyltransferase complex	5.93E−04
GO:1900024	regulation of substrate adhesion-dependent cell spreading	5.93E−04
GO:1901020	negative regulation of calcium ion transmembrane transporter activity	5.93E−04
GO:51131	chaperone-mediated protein complex assembly	5.93E−04
GO:42288	MHC class I protein binding	5.93E−04
GO:7051	spindle organization	6.10E−04
GO:9725	response to hormone stimulus	6.14E−04
GO:44766	multi-organism transport	6.28E−04
GO:72512	trivalent inorganic cation transport	6.28E−04
GO:33572	transferrin transport	6.28E−04
GO:15682	ferric iron transport	6.28E−04
GO:16655	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor	6.34E−04
GO:16829	lyase activity	6.38E−04
GO:6308	DNA catabolic process	6.84E−04
GO:43022	ribosome binding	6.86E−04
GO:50776	regulation of immune response	7.10E−04
GO:10639	negative regulation of organelle organization	7.12E−04
GO:43413	macromolecule glycosylation	7.19E−04
GO:6466	protein glycosylation	7.19E−04
GO:5839	proteasome core complex	7.27E−04
GO:5852	eukaryotic translation initiation factor 3 complex	7.27E−04
GO:30866	cortical actin cytoskeleton organization	7.31E−04
GO:786	nucleosome	7.32E−04
GO:2161	aminoacyl-tRNA editing activity	7.53E−04
GO:70419	nonhomologous end joining complex	7.61E−04
GO:8429	phosphatidylethanolamine binding	7.61E−04
GO:5589	collagen type VI	7.61E−04
GO:6101	citrate metabolic process	7.61E−04
GO:15037	peptide disulfide oxidoreductase activity	7.61E−04
GO:43488	regulation of mRNA stability	7.62E−04
GO:9142	nucleoside triphosphate biosynthetic process	7.77E−04
GO:32273	positive regulation of protein polymerization	7.84E−04
GO:2684	positive regulation of immune system process	7.87E−04
GO:10008	endosome membrane	8.26E−04
GO:45807	positive regulation of endocytosis	8.26E−04
GO:1937	negative regulation of endothelial cell proliferation	8.44E−04
GO:42339	keratan sulfate metabolic process	8.44E−04
GO:45121	membrane raft	8.48E−04
GO:10563	negative regulation of phosphorus metabolic process	8.73E−04
GO:45936	negative regulation of phosphate metabolic process	8.73E−04
GO:32409	regulation of transposer activity	8.76E−04
GO:9108	coenzyme biosynthetic process	8.76E−04
GO:2253	activation of immune response	8.78E−04
GO:30336	negative regulation of cell migration	8.79E−04
GO:6875	cellular metal ion homeostasis	9.06E−04
GO:40013	negative regulation of locomotion	9.14E−04
GO:33673	negative regulation of kinase activity	9.18E−04
GO:51924	regulation of calcium ion transport	9.20E−04
GO:30705	cytoskeleton-dependent intracellular transport	9.26E−04
GO:43256	laminin complex	9.32E−04
GO:19471	4-hydroxyproline metabolic process	9.32E−04
GO:1901021	positive regulation of calcium ion transmembrane transporter activity	9.32E−04
GO:42273	ribosomal large subunit biogenesis	9.32E−04
GO:6103	2-oxoglutarate metabolic process	9.32E−04
GO:7009	plasma membrane organization	9.57E−04
GO:51099	positive regulation of binding	9.80E−04
GO:2000146	negative regulation of cell motility	1.01E−03
GO:44325	ion channel binding	1.01E−03
GO:1901605	alpha-amino acid metabolic process	1.03E−03
GO:44070	regulation of anion transport	1.04E−03
GO:70085	glycosylation	1.06E−03
GO:6022	aminoglycan metabolic process	1.08E−03
GO:5884	actin filament	1.09E−03
GO:10740	positive regulation of intracellular protein kinase cascade	1.10E−03
GO:70206	protein trimerization	1.11E−03
GO:31418	L-ascorbic acid binding	1.11E−03
GO:30865	cortical cytoskeleton organization	1.11E−03
GO:5911	cell-cell junction	1.13E−03
GO:42493	response to drug	1.13E−03
GO:18904	ether metabolic process	1.14E−03
GO:45022	early endosome to late endosome transport	1.15E−03
GO:5836	proteasome regulatory particle	1.15E−03
GO:5853	eukaryotic translation elongation factor 1 complex	1.15E−03
GO:43024	ribosomal small subunit binding	1.15E−03
GO:44597	daunorubicin metabolic process	1.15E−03
GO:44598	doxorubicin metabolic process	1.15E−03
GO:44183	protein binding involved in protein folding	1.15E−03
GO:51016	barbed-end actin filament capping	1.15E−03
GO:30647	aminoglycoside antibiotic metabolic process	1.15E−03
GO:4300	enoyl-CoA hydratase activity	1.15E−03
GO:75713	establishment of integrated proviral latency	1.15E−03
GO:15266	porin activity	1.15E−03
GO:46487	glyoxylate metabolic process	1.15E−03
GO:51015	actin filament binding	1.16E−03
GO:43484	regulation of RNA splicing	1.17E−03
GO:4866	endopeptidase inhibitor activity	1.20E−03
GO:71824	protein-DNA complex subunit organization	1.20E−03
GO:10975	regulation of neuron projection development	1.21E−03
GO:19897	extrinsic to plasma membrane	1.22E−03
GO:35637	multicellular organsimal signaling	1.22E−03
GO:61136	regulation of proteasomal protein catabolic process	1.23E−03
GO:19903	protein phosphatase binding	1.23E−03
GO:8144	drug binding	1.23E−03
GO:61383	trabecula morphogenesis	1.24E−03
GO:33017	sarcoplasmic reticulum membrane	1.25E−03
GO:5605	basal lamina	1.25E−03
GO:50688	regulation of defense response to virus	1.25E−03
GO:42058	regulation of epidermal growth factor receptor signaling pathway	1.25E−03
GO:16705	oxidoreductase activity, acting on paired donors, with incorporation of reduction of molecular	1.27E−03
	oxygen
GO:31344	regulation of cell projection organization	1.28E−03
GO:52632	citrate hydro-lyase (cis-aconitate-forming) activity	1.30E−03
GO:52633	isocitrate hydro-lyase (cis-aconitate-forming) activity	1.30E−03
GO:70288	ferritin complex	1.30E−03
GO:461	endonucleolytic cleavage to generate mature 3′-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA,	1.30E−03
	LSU-rRNA)
GO:35613	RNA stem-loop binding	1.30E−03
GO:17098	sulfonylurea receptor binding	1.30E−03
GO:2135	CTP binding	1.30E−03
GO:2580	regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC	1.30E−03
	class II
GO:10501	RNA secondary structure unwinding	1.30E−03
GO:1901844	regulation of cell communication by electrical coupling involved in cardiac conduction	1.30E−03
GO:5584	collagen type I	1.30E−03
GO:5999	xylulose biosynthetic process	1.30E−03
GO:6097	glyoxylate cycle	1.30E−03
GO:5642	annulate lamellae	1.30E−03
GO:6880	intracellular sequestering of iron ion	1.30E−03
GO:2001135	regulation of endocytic recycling	1.30E−03
GO:2001137	positive regulation of endocytic recycling	1.30E−03
GO:31372	UBC13-MMS2 complex	1.30E−03
GO:8043	intracellular ferritin complex	1.30E−03
GO:43524	negative regulation of neuron apoptotic process	1.32E−03
GO:1901184	regulation of ERBB signaling pathway	1.33E−03
GO:50778	positive regulation of immune response	1.35E−03
GO:43487	regulation of RNA stability	1.35E−03
GO:9063	branched-chain amino acid catabolic process	1.36E−03
GO:4033	aldo-keto reductase (NADP) activity	1.36E−03
GO:44297	cell body	1.36E−03
GO:61135	endopeptidase regulator activity	1.37E−03
GO:43588	skin development	1.44E−03
GO:31674	I band	1.45E−03
GO:5765	lysosomal membrane	1.45E−03
GO:8202	steroid metabolic process	1.46E−03
GO:6469	negative regulation of protein kinase activity	1.46E−03
GO:2831	regulation of response to biotic stimulus	1.46E−03
GO:15662	ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism	1.47E−03
GO:6323	DNA packaging	1.51E−03
GO:7229	integrin-mediated signaling pathway	1.51E−03
GO:55065	metal ion homeostasis	1.54E−03
GO:32412	regulation of ion transmembrane transporter activity	1.55E−03
GO:30027	lamellipodium	1.57E−03
GO:9890	negative regulation of biosynthetic process	1.59E−03
GO:43171	peptide catabolic process	1.63E−03
GO:33018	sarcoplasmic reticulum lumen	1.63E−03
GO:50786	RAGE receptor binding	1.63E−03
GO:303	response to superoxide	1.63E−03
GO:1516	prostaglandin biosynthetic process	1.63E−03
GO:46457	prostanoid biosynthetic process	1.63E−03
GO:97202	activation of cysteine-type endopeptidase activity	1.65E−03
GO:32508	DNA duplex unwinding	1.65E−03
GO:16620	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as	1.65E−03
	acceptor
GO:6767	water-soluble vitamin metabolic process	1.65E−03
GO:70161	anchoring junction	1.66E−03
GO:45595	regulation of cell differentiation	1.66E−03
GO:51348	negative regulation of transferase activity	1.68E−03
GO:9615	response to virus	1.68E−03
GO:30414	peptidase inhibitor activity	1.69E−03
GO:34097	response to cytokine stimulus	1.72E−03
GO:785	chromatin	1.73E−03
GO:31256	leading edge membrane	1.74E−03
GO:32414	positive regulation of ion transmembrane transporter activity	1.76E−03
GO:45259	proton-transporting ATP synthase complex	1.76E−03
GO:71705	nitrogen compound transport	1.77E−03
GO:19395	fatty acid oxdation	1.77E−03
GO:31547	regulation of protein stability	1.81E−03
GO:32392	DNA geometric change	1.85E−03
GO:7162	negative regulation of cell adhesion	1.85E−03
GO:34440	lipid oxidation	1.90E−03
GO:8015	blood circulation	1.91E−03
GO:30506	ankyrin binding	1.93E−03
GO:7517	muscle organ development	1.96E−03
GO:30262	apoptotic nuclear changes	1.97E−03
GO:7568	aging	2.02E−03
GO:10959	regulation of metal ion transport	2.05E−03
GO:1901215	negative regulation of neuron death	2.07E−03
GO:3013	circulatory system process	2.07E−03
GO:16903	oxidoreductase activity, acting on the aldehyde or oxo group of donors	2.07E−03
GO:33293	monocarboxylic acid binding	2.08E−03
GO:16597	amino acid binding	2.16E−03
GO:42277	peptide binding	2.17E−03
GO:38127	ERBB signaling pathway	2.17E−03
GO:34332	adherens junction organization	2.18E−03
GO:8233	peptidase activity	2.18E−03
GO:8289	lipid binding	2.18E−03
GO:34333	adherens junction assembly	2.20E−03
GO:48024	regulation of mRNA splicing, via spliceosome	2.20E−03
GO:15985	energy coupled proton transport, down electrochemical gradient	2.20E−03
GO:15986	ATP synthesis coupled proton transport	2.20E−03
GO:16417	S-acyltransferase activity	2.21E−03
GO:10592	positive regulation of lamellipodium assembly	2.21E−03
GO:19043	establishment of viral latency	2.21E−03
GO:6011	UDP-glucose metabolic process	2.21E−03
GO:5652	nuclear lamina	2.21E−03
GO:8091	spectrin	2.21E−03
GO:50684	regulation of mRNA processing	2.25E−03
GO:34080	CENP-A containing nucleosome assembly at centromere	2.26E−03
GO:34724	DNA replication-independent nucleosome organization	2.26E−03
GO:34308	primary alcohol metabolic process	2.26E−03
GO:6336	DNA replication-independent nucleosome assembly	2.26E−03
GO:32413	negative regulation of ion transmembrane transporter activity	2.26E−03
GO:18208	peptidyl-proline modification	2.30E−03
GO:19898	extrinsic to membrane	2.32E−03
GO:61061	muscle structure development	2.33E−03
GO:2433	immune response-regulating cell surface receptor signaling pathway involved in phagocytosis	2.41E−03
GO:38094	Fc-gamma receptor signaling pathway	2.41E−03
GO:38096	Fc-gamma receptor signaling pathway involved in phagocytosis	2.41E−03
GO:30018	Z disc	2.42E−03
GO:47485	protein N-terminus bindng	2.43E−03
GO:6766	vitamin metabolic process	2.43E−03
GO:16363	nuclear matrix	2.43E−03
GO:6090	pyruvate metabolic process	2.43E−03
GO:5912	adherens junction	2.43E−03
GO:30335	positive regulation of cell migration	2.51E−03
GO:51494	negative regulation of cytoskeleton organization	2.57E−03
GO:33218	amide binding	2.60E−03
GO:2431	Fc receptor mediated stimulatory signaling pathway	2.60E−03
GO:4003	ATP-dependent DNA helicase activity	2.63E−03
GO:2001259	positive regulation of cation channel activity	2.64E−03
GO:42326	negative regulation of phosphorylation	2.65E−03
GO:45806	negative regulation of endocytosis	2.70E−03
GO:10769	regulation of cell morphogenesis involved in differentiation	2.72E−03
GO:10720	positive regulation of cell development	2.72E−03
GO:70011	peptidase activity, acting on L-amino acid peptides	2.76E−03
GO:19226	transmission of nerve impulse	2.77E−03
GO:9161	ribonucleoside monophosphate metabolic process	2.79E−03
GO:71229	cellular response to acid	2.79E−03
GO:48678	response to axon injury	2.79E−03
GO:32434	regulation of proteasomal ubiquitin-dependent protein catabolic process	2.79E−03
GO:43523	regulation of neuron apoptotic process	2.80E−03
GO:71230	cellular response to amino acid stimulus	2.84E−03
GO:38093	Fc receptor signaling pathway	2.90E−03
GO:43295	glutathione binding	2.90E−03
GO:51409	response to nitrosative stress	2.90E−03
GO:1846	complement binding	2.90E−03
GO:17166	vinculin binding	2.90E−03
GO:1900750	oligopeptide binding	2.90E−03
GO:33483	gas homeostasis	2.90E−03
GO:19042	viral latency	2.90E−03
GO:6102	isocitrate metabolic process	2.90E−03
GO:6069	ethanol oxidation	2.90E−03
GO:6610	ribosomal protein import into nucleus	2.90E−03
GO:6189	‘de novo’ IMP biosynthetic process	2.90E−03
GO:31904	endosome lumen	2.90E−03
GO:2000147	positive regulation of cell motility	2.93E−03
GO:51017	actin filament bundle assembly	2.95E−03
GO:43034	costamere	3.03E−03
GO:42162	telomeric DNA binding	3.03E−03
GO:8285	negative regulation of cell proliferation	3.09E−03
GO:1901699	cellular response to nitrogen compound	3.10E−03
GO:7163	establishment of maintenance of cell polarity	3.10E−03
GO:6826	iron ion transport	3.14E−03
GO:30234	enzyme regulator activity	3.14E−03
GO:71248	cellular response to metal ion	3.14E−03
GO:45335	phagocytic vesicle	3.14E−03
GO:30674	protein binding, bridging	3.22E−03
GO:51346	negative regulation of hydrolase activity	3.23E−03
GO:1556	regulation of cell growth	3.26E−03
GO:71277	cellular response to calcium ion	3.27E−03
GO:44455	mitochondrial membrane part	3.33E−03
GO:51020	GTPase binding	3.41E−03
GO:70458	cellular detoxification of nitrogen compound	3.41E−03
GO:9169	purine ribonucleoside monophosphate catabolic process	3.41E−03
GO:9158	ribonucleoside monophosphate catabolic process	3.41E−03
GO:43200	laminin-11 complex	3.41E−03
GO:71438	invadopodium membrane	3.41E−03
GO:16672	oxidoreductase activity, acting on a sulfur group of donors, quinone or similar compound as	3.41E−03
	acceptor
GO:1884	pyrimidine nucleoside binding	3.41E−03
GO:2134	UTP binding	3.41E−03
GO:19797	procollagen-proline 3-dioxygenase activity	3.41E−03
GO:10716	negative regulation of extracellular matrix disassembly	3.41E−03
GO:33484	nitric oxide homeostasis	3.41E−03
GO:18636	phenanthrene 9,10-monooxygenase activity	3.41E−03
GO:18916	nitrobenzene metabolic process	3.41E−03
GO:1901741	positive regulation of myoblast fusion	3.41E−03
GO:3994	aconitate hydratase activity	3.41E−03
GO:47718	indanol dehydrogenase activity	3.41E−03
GO:6106	fumarate metabolic process	3.41E−03
GO:6065	UDP-glucuronate biosynthetic process	3.41E−03
GO:32557	pyrimidine ribonucleotide binding	3.41E−03
GO:32551	pyrimidine ribonucleoside binding	3.41E−03
GO:31838	haptoglobin-hemoglobin complex	3.41E−03
GO:31544	peptidyl-proline 3-dioxygenase activity	3.41E−03
GO:47086	ketosteroid monooxygenase activity	3.41E−03
GO:15853	adenine transport	3.41E−03
GO:31125	rRNA 3′-end processing	3.41E−03
GO:43277	apoptotic cell clearance	3.43E−03
GO:17134	fibroblast growth factor binding	3.43E−03
GO:5523	tropomyosin binding	3.43E−03
GO:6188	IMP biosynthetic process	3.43E−03
GO:31055	chromatin remodeling at centromere	3.43E−03
GO:1523	retinoid metabolic process	3.47E−03
GO:16706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular	3.47E−03
	oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both
	donors
GO:3678	DNA helicase activity	3.47E−03
GO:8308	voltage-gated anion channel activity	3.53E−03
GO:36296	response to increased oxygen levels	3.53E−03
GO:35872	nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway	3.53E−03
GO:55093	response to hyperoxia	3.53E−03
GO:19902	phosphatase binding	3.54E−03
GO:1901681	sulfur compound binding	3.56E−03
GO:8536	Ran GTPase binding	3.65E−03
GO:16461	unconventional myosin complex	3.65E−03
GO:19652	L-ascorbic acid metabolic process	3.65E−03
GO:34310	primary alcohol catabolic process	3.65E−03
GO:32456	endocytic recycling	3.65E−03
GO:31996	thioesterase binding	3.65E−03
GO:43169	cation binding	3.66E−03
GO:6687	glycosphingolipid metabolic process	3.66E−03
GO:71417	cellular response to organic nitrogen	3.72E−03
GO:42625	ATPase activity, coupled to transmembrane movement of ions	3.84E−03
GO:7167	enzyme linked receptor protein signaling pathway	3.90E−03
GO:43489	RNA stabilization	3.91E−03
GO:51764	actin crosslink formation	3.91E−03
GO:2102	podosome	3.91E−03
GO:30127	COPII vesicle coat	3.91E−03
GO:48255	mRNA stabilization	3.91E−03
GO:46040	IMP metabolic process	3.91E−03
GO:40017	positive regulation of locomotion	3.93E−03
GO:9991	response to extracellular stimulus	3.96E−03
GO:42445	hormone metabolic process	4.02E−03
GO:71241	cellular response to inorganic substance	4.16E−03
GO:33993	response to lipid	4.17E−03
GO:90382	phagosome maturation	4.24E−03
GO:723	telomere maintenance	4.28E−03
GO:5977	glycogen metabolic process	4.28E−03
GO:31123	RNA 3′-end processing	4.37E−03
GO:31345	negative regulation of cell projection organization	4.39E−03
GO:43269	regulation of ion transport	4.39E−03
GO:1901214	regulation of neuron death	4.44E−03
GO:51059	NF-kappaB binding	4.45E−03
GO:34314	Arp2/3 complex-mediated actin nucleation	4.45E−03
GO:45182	translation regulator activity	4.45E−03
GO:60284	regulation of cell development	4.53E−03
GO:44042	glucan metabolic process	4.57E−03
GO:72341	modified amino acid binding	4.57E−03
GO:30048	actin filament-based movement	4.57E−03
GO:6073	cellular glucan metabolic process	4.57E−03
GO:32200	telomere organization	4.57E−03
GO:51094	positive regulation of developmental process	4.57E−03
GO:36295	cellular response to increased oxygen levels	4.57E−03
GO:71455	cellular response to hyperoxia	4.57E−03
GO:60346	bone trabecula formation	4.57E−03
GO:50765	negative regulation of phagocytosis	4.57E−03
GO:30214	hyaluronan catabolic process	4.57E−03
GO:7268	synaptic transmission	4.69E−03
GO:51188	cofactor biosynthetic process	4.69E−03
GO:5539	glycosaminoglycan binding	4.69E−03
GO:31346	positive regulation of cell projection organization	4.69E−03
GO:51092	positive regulation of NF-kappaB transcription factor activity	4.77E−03
GO:44264	cellular polysaccharide metabolic process	4.85E−03
GO:16101	diterpenoid metabolic process	4.85E−03
GO:1676	long-chain fatty acid metabolic process	5.01E−03
GO:45010	actin nucleation	5.01E−03
GO:51881	regulation of mitochondrial membrane potential	5.01E−03
GO:5916	fascia adherens	5.01E−03
GO:9168	purine ribonucleoside monophosphate biosynthetic process	5.01E−03
GO:9127	purine nucleoside monophosphate biosynthetic process	5.01E−03
GO:19432	triglyceride biosynthetic process	5.01E−03
GO:3684	damaged DNA binding	5.24E−03
GO:51174	regulation of phosphorus metabolic process	5.28E−03
GO:45111	intermediate filament cytoskeleton	5.30E−03
GO:6919	activation of cysteine-type endopeptidase activity involved in apoptotic process	5.41E−03
GO:6944	cellular membrane fusion	5.41E−03
GO:43900	regulation of multi-organism process	5.47E−03
GO:7040	lysosome organization	5.47E−03
GO:16469	proton-transporting two-sector ATPase complex	5.48E−03
GO:90316	positive regulation of intracellular protein transport	5.48E−03
GO:10558	negative regulation of macromolecule biosynthetic process	5.56E−03
GO:8360	regulation of cell shape	5.57E−03
GO:1901615	organic hydroxy compound metabolic process	5.58E−03
GO:18401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline	5.63E−03
GO:90136	epithelial cell-cell adhesion	5.63E−03
GO:5719	nuclear euchromatin	5.63E−03
GO:16137	glycoside metabolic process	5.63E−03
GO:31258	lamellipodium membrane	5.63E−03
GO:9081	branched-chain amino acid metabolic process	5.63E−03
GO:43466	histone exchange	5.63E−03
GO:48365	Rac GTPase binding	5.63E−03
GO:32410	negative regulation of transporter activity	5.63E−03
GO:4197	cysteine-type endopeptidase activity	5.69E−03
GO:44769	ATPase activity, coupled to transmembrane movement of ions, rotational mechanism	5.91E−03
GO:46463	acylglycerol biosynthetic process	5.91E−03
GO:46460	neutral lipid biosynthetic process	5.91E−03
GO:45216	cell-cell junction organization	5.94E−03
GO:31327	negative regulation of cellular biosynthetic process	6.05E−03
GO:44389	small conjugating protein ligase binding	6.12E−03
GO:31625	uibiquitin protein ligase binding	6.12E−03
GO:71345	cellular response to cytokine stimulus	6.15E−03
GO:34754	cellular hormone metabolic process	6.19E−03
GO:447	endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from	6.19E−03
	tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:478	endonucleolytic cleavage involved in rRNA processing	6.19E−03
GO:479	endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	6.19E−03
GO:469	cleavage involved in rRNA processing	6.19E−03
GO:9128	purine nucleoside monophosphate catabolic process	6.19E−03
GO:70061	fructose binding	6.19E−03
GO:8426	protein kinase C inhibitor activity	6.19E−03
GO:9753	response to jasmonic acid stimulus	6.19E−03
GO:45040	protein import into mitochondrial outer membrane	6.19E−03
GO:51683	establishment of Golgi localization	6.19E−03
GO:51208	sequestering of calcium ion	6.19E−03
GO:36021	endolysosome lumen	6.19E−03
GO:71395	cellular response to jasmonic acid stimulus	6.19E−03
GO:34205	beta-amyloid formation	6.19E−03
GO:1901739	regulation of myoblast fusion	6.19E−03
GO:47115	trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity	6.19E−03
GO:90502	RNA phosphodiester bond hydrolysis, endonucleolytic	6.19E−03
GO:5997	xylulose metabolic process	6.19E−03
GO:5862	muscle thin filament tropomyosin	6.19E−03
GO:7161	calcium-independent cell-matrix adhesion	6.19E−03
GO:45252	oxoglutarate dehydrogenase complex	6.19E−03
GO:7008	outer mitochondrial membrane organization	6.19E−03
GO:47023	androsterone dehydrogenase activity	6.19E−03
GO:46294	formaldehyde catabolic process	6.19E−03
GO:46398	UDP-glucuronate metabolic process	6.19E−03
GO:17025	TBP-class protein binding	6.23E−03
GO:6309	apoptotic DNA fragmentation	6.23E−03
GO:6906	vesicle fusion	6.23E−03
GO:60090	binding, bridging	6.23E−03
GO:7173	epidermal growth factor receptor signaling pathway	6.46E−03
GO:61025	membrane fusion	6.54E−03
GO:2757	immune response-activating signal transduction	6.65E−03
GO:8565	protein transporter activity	6.65E−03
GO:16801	hydrolase activity, acting on ether bonds	6.71E−03
GO:51196	regulation of coenzyme metabolic process	6.71E−03
GO:51193	regulation of cofactor metabolic process	6.71E−03
GO:97066	response to thyroid hormone stimulus	6.71E−03
GO:42136	neurotransmitter biosynthetic process	6.71E−03
GO:4656	procollagen-proline 4-dioxygenase activity	6.71E−03
GO:14912	negative regulation of smooth muscle cell migration	6.71E−03
GO:10770	positive regulation of cell morphogenesis involved in differentiation	6.82E−03
GO:737	DNA catabolic process, endonucleolytic	6.94E−03
GO:34508	centromere complex assembly	6.94E−03
GO:6693	prostaglandin metabolic process	6.94E−03
GO:6692	prostanoid metabolic process	6.94E−03
GO:8186	RNA-dependent ATPase activity	6.94E−03
GO:46209	nitric oxide metabolic process	6.94E−03
GO:5901	caveola	6.98E−03
GO:9607	response to biotic stimulus	7.01E−03
GO:30055	cell-substrate junction	7.04E−03
GO:15078	hydrogen ion transmembrane transporter activity	7.04E−03
GO:30216	keratinocyte differentiation	7.20E−03
GO:1901606	alpha-amino acid catabolic process	7.30E−03
GO:16324	apical plasma membrane	7.49E−03
GO:70469	respiratory chain	7.58E−03
GO:5976	polysaccharide metabolic process	7.58E−03
GO:51707	response to other organism	7.59E−03
GO:2000113	negative regulation of cellular macromolecule biosynthetic process	7.60E−03
GO:60351	cartilage development involved in endochondral bone morphogenesis	7.72E−03
GO:44275	cellular carbohydrate catabolic process	7.72E−03
GO:48041	focal adhesion assembly	7.72E−03
GO:30212	hyaluronan metabolic process	7.72E−03
GO:7017	microtubule-based process	7.94E−03
GO:30145	manganese ion binding	7.94E−03
GO:5882	intermediate filament	7.99E−03
GO:31667	response fo nutrient levels	7.99E−03
GO:9113	purine nucleobase biosynthetic process	7.99E−03
GO:19511	peptidyl-proline hydroxylation	7.99E−03
GO:90075	relaxation of muscle	7.99E−03
GO:51156	glucose 6-phosphate metabolic process	7.99E−03
GO:2000811	negative regulation of anoikis	7.99E−03
GO:5527	macrolide binding	7.99E−03
GO:5528	FK506 binding	7.99E−03
GO:51172	negative regulation of nitrogen compound metabolic process	8.06E−03
GO:10817	regulation of hormone levels	8.19E−03
GO:9123	nucleoside monophosphate metabolic process	8.31E−03
GO:2764	immune response-regulating signaling pathway	8.52E−03
GO:32436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process	8.52E−03
GO:43236	laminin binding	8.53E−03
GO:4602	glutathione peroxidase activity	8.53E−03
GO:5504	fatty acid binding	8.53E−03
GO:55038	recycling endosome membrane	8.53E−03
GO:790	nuclear chromatin	8.68E−03
GO:6029	proteoglycan metabolic process	8.71E−03
GO:60491	regulation of cell projection assembly	8.79E−03
GO:50821	protein stabilization	8.79E−03
GO:6626	protein targeting to mitochondrion	8.79E−03
GO:8610	lipid biosynthetic process	8.90E−03
GO:3743	translation initiation factor activity	8.94E−03
GO:2429	immune response-activating cell surface receptor signaling pathway	9.02E−03
GO:19674	NAD metabolic process	9.12E−03
GO:50766	positive regulation of phagocytosis	9.12E−03
GO:1901570	fatty acid derivative biosynthetic process	9.12E−03
GO:5791	rough endoplasmic reticulum	9.12E−03
GO:7266	Rho protein signal transduction	9.12E−03
GO:46456	icosanoid biosynthetic process	9.12E−03
GO:17016	Ras GTPase binding	9.16E−03
GO:33157	regulation of intracellular protein transport	9.16E−03
GO:46683	response to organophosphorus	9.29E−03
GO:32101	regulation of response to external stimulus	9.38E−03
GO:62	fatty-acyl-CoA binding	9.38E−03
GO:43124	negative regulation of I-kappaB kinase/NF-kappaB cascade	9.38E−03
GO:42287	MHC protein binding	9.38E−03
GO:46961	proton-transporting ATPase activity, rotational mechanism	9.38E−03
GO:35304	regulation of protein dephosphorylation	9.38E−03
GO:791	euchromatin	9.38E−03
GO:10591	regulation of lamellipodium assembly	9.38E−03
GO:6067	ethanol metabolic process	9.38E−03
GO:19829	cation-transporting ATPase activity	9.46E−03
GO:18119	peptidyl-cysteine S-nitrosylation	9.52E−03
GO:16418	S-acetyltransferase activity	9.52E−03
GO:36125	fatty acid beta-oxidation multienzyme complex	9.52E−03
GO:16507	mitochondrial fatty acid beta-oxidation multienzyme complex	9.52E−03
GO:16509	long-chain-3-hydroxyacyl-CoA dehydrogenase activity	9.52E−03
GO:60143	positive regulation of syncytium formation by plasma membrane fusion	9.52E−03
GO:51410	detoxification of nitrogen compound	9.52E−03
GO:16744	transferase activity, transferring aldehyde or ketonic groups	9.52E−03
GO:17070	U6 snRNA binding	9.52E−03
GO:1765	membrane raft assembly	9.52E−03
GO:17014	protein nitrosylation	9.52E−03
GO:1849	complement component C1q binding	9.52E−03
GO:70836	caveola assembly	9.52E−03
GO:1901163	regulation of trophoblast cell migration	9.52E−03
GO:71682	endocytic vesicle lumen	9.52E−03
GO:34112	positive regulation of homotypic cell-cell adhesion	9.52E−03
GO:4301	epoxide hydrolase activity	9.52E−03
GO:4822	isoleucine-tRNA ligase activity	9.52E−03
GO:90501	RNA phosphodiester bond hydrolysis	9.52E−03
GO:6428	isoleucyl-tRNA aminoacylation	9.52E−03
GO:32437	cuticular plate	9.52E−03
GO:14809	regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion	9.52E−03
GO:38069	positive regulation of cell migration by vascular endothelial growth factor signaling pathway	9.52E−03
GO:46790	virion binding	9.52E−03
GO:31340	positive regulation of vesicle fusion	9.52E−03
GO:46292	formaldehyde metabolic process	9.52E−03
GO:30911	TPR domain binding	9.52E−03
GO:72330	monocarboxylic acid biosynthetic process	9.53E−03
GO:1901800	positive regulation of proteasomal protein catabolic process	9.60E−03
GO:2020	protease binding	9.61E−03
GO:72655	establishment of protein localization to mitochondrion	9.61E−03
GO:32479	regulation of type I interferon production	9.61E−03
GO:6259	DNA metabolic process	9.73E−03
GO:71453	cellular response to oxygen levels	9.78E−03
GO:71559	response to transforming growth factor beta stimulus	9.79E−03
GO:6694	steroid biosynthetic process	9.85E−03
GO:31965	nuclear membrane	9.91E−03

TABLE 3
Lists of ECM and organogenesis/morphogenesis proteins identified in both
engineered and native VF mucosae. Identifications were based on a 1% false discovery rate.
Corresponding heatmaps are presented in FIG. 3F. Proteins are listed in order of descending
fold change (native VF mucosa compared to engineered VF mucosa).
Gene symbol	Protein name	Fold change
ECM proteins
ALB	Serum albumin	28.43
TNXB	Tenascin-X	17.07
COL5A1	Collagen alpha-1(V) chain	14.10
DPYSL3	Dihydropyrimidinase-related protein 3	9.79
FBLN1	Fibulin-1	7.39
FBN1	Fibrillin-1	6.16
SERPINB1	Leukocyte elastase inhibitor	5.57
CLU	Clusterin	4.82
BGN	Biglycan	4.77
HSPG2	Basement membrane-specific heparan sulfate proteoglycan core protein	3.25
TGFBI	Transforming growth factor-beta-induced protein ig-h3	3.25
CD109	CD109 antigen	3.22
B2M	Beta-2-microglobulin	3.07
HMGB1	High mobility group protein B1	2.53
COL18A1	Collagen alpha-1(XVIII) chain	2.35
LUM	Lumican	2.27
COL6A2	Collagen alpha-2(VI) chain	2.12
DCN	Decorin	1.88
COL6A1	Collagen alpha-1(VI) chain	1.65
LGALS3	Galectin-3	1.63
MYH9	Myosin-9	1.62
STOM	Erythrocyte band 7 integral membrane protein	1.60
ANXA1	Annexin A1	1.59
PCYOX1	Prenylcysteine oxidase 1	1.51
ACTN4	Alpha-actinin-4	1.50
HSPD1	60 kDa heat shock protein, mitochondrial	1.49
CTSD	Cathepsin D	1.47
CLTC	Clathrin heavy chain 1	1.36
COL6A3	Collagen alpha-3(VI) chain	1.35
GSN	Gelsolin	1.26
GPI	Glucose-6-phosphate isomerase	1.21
SOD1	Superoxide dismutase [Cu—Zn]	1.15
ALDOA	Fructose-bisphosphate aldolase A	1.11
ANXA5	Annexin A5	1.10
LAMA5	Laminin subunit alpha-5	1.08
ANXA2	Annexin A2	1.00
ENO1	Alpha-enolase	0.99
FBLN2	Fibulin-2	0.99
TUBB	Tubulin beta chain	0.97
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	0.96
HSPA8	Heat shock cognate 71 kDa protein	0.95
CALR	Calreticulin	0.95
CTSB	Cathepsin B	0.91
ACTN1	Alpha-actinin-1	0.91
VIM	Vimentin	0.82
COL3A1	Collagen alpha-1(III) chain	0.81
MSN	Moesin	0.81
PRDX4	Peroxiredoxin-4	0.71
MVP	Major vault protein	0.71
PKM	Pyruvate kinase isozymes M1/M2	0.70
ANXA6	Annexin A6	0.69
DYNC1H1	Cytoplasmic dynein 1 heavy chain 1	0.65
AKR1B1	Aldose reductase	0.64
AHSG	Alpha-2-HS-glycoprotein	0.63
COPA	Coatomer subunit alpha	0.61
FN1	Fibronectin	0.55
FLNA	Filamin-A	0.51
RNH1	Ribonuclease inhibitor	0.50
RBMX	RNA-binding motif protein, X chromosome	0.50
ITGB1	Integrin beta-1	0.50
MYOF	Myoferlin	0.47
LGALS3BP	Galectin-3-binding protein	0.45
LGALS1	Galectin-1	0.44
MYO1C	Unconventional myosin-Ic	0.41
THBS1	Thrombospondin-1	0.35
LAMC1	Laminin subunit gamma-1	0.34
HDGF	Hepatoma-derived growth factor	0.31
EMILIN1	EMILIN-1	0.31
SERPINF1	Pigment epithelium-derived factor	0.26
TLN1	Talin-1	0.25
NT5E	5′-nucleotidase	0.20
COL12A1	Collagen alpha-1(XIII) chain	0.19
PSAP	Proactivator polypeptide	0.19
HDLBP	Vigilin	0.16
COL1A2	Collagen alpha-2(I) chain	0.09
COL1A1	Collagen alpha-1(I) chain	0.07
Organogenesis/morphogenesis proteins
JUP	Junction plakoglobin	84.57
KRT5	Keratin, type II cytoskeletal 5	32.13
PPL	Periplakin	23.40
KRT14	Keratin, type I cytoskeletal 14	22.50
KRT6B	Keratin, type II cytoskeletal 6B	16.64
COL5A1	Collagen alpha-1(V) chain	10.13
STEAP4	Metalioreductase STEAP4	8.08
DPYSL3	Dihydropyrimidinase-related protein 3	6.97
FBLN1	Fibulin-1	5.27
OGDH	2-oxoglutarate dehydrogenase, mitochondrial	5.26
FBN1	Fibrillin-1	4.36
DLD	Dihydrolipoyl dehydrogenase, mitochondrial	3.47
ATP2A2	Sarcoplasmic/endoplasmic reticulum calcium ATPase 2	3.45
SOD2	Superoxide dismutase [Mn], mitochondrial	2.95
PRDX3	Thioredoxin-dependent peroxide reductase, mitochondrial	2.48
ASAH1	Acid ceramidase	2.44
RAB11A	Ras-related protein Rab-11A	2.41
HSPG2	Basement membrane-specific heparan sulfate proteoglycan core protein	2.31
ADD1	Alpha-adducin	2.29
TGFBI	Transforming growth factor-beta-induced protein ig-h3	2.29
B2M	Beta-2-microglobulin	2.19
KRT10	Keratin, type I cytoskeletal 10	1.85
HMGB1	High mobility group protein B1	1.79
COL18A1	Collagen alpha-1(XVIII) chain	1.65
NPM1	Nucleophosmin	1.65
LUM	Lumican	1.61
AP2A2	AP-2 complex subunit alpha-2	1.60
PHB2	Prohibitin-2	1.53
COL6A2	Collagen alpha-2(VI) chain	1.49
FERMT2	Fermitin family homolog 2	1.48
ATP5A1	ATP synthase subunit alpha, mitochondrial	1.34
DCN	Deconin	1.33
XRCC5	X-ray repair cross-complementing protein 5	1.29
SPTAN1	Spectrin alpha chain, non-erythrocylic 1	1.26
SPTBN1	Spectrin beta chain, non-erythrocytic 1	1.25
COL6A1	Collagen alpha-1(VI) chain	1.16
TPP1	Tripeptidyl-peptidase 1	1.16
LGALS3	Galectin-3	1.15
MYH9	Myosin-9	1.15
RPS14	40S ribosomal protein S14	1.14
ANXA1	Annexin A1	1.12
SRI	Sorcin	1.10
NDRG1	Protein NDRG1	1.10
ATP5B	ATP synthase subunit beta, mitochondrial	1.08
HSPD1	60 kDa heat shock protein, mitochondrial	1.06
CANX	Calnexin	1.02
RPS4X	40S ribosomal protein S4, X isoform	1.00
APEX1	DNA-(apurinic or apyrimidinic site) lyase	0.99
COL6A3	Collagen alpha-3(VI) chain	0.95
RPN2	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2	0.92
CDC42	Cell division control protein 42 homolog	0.91
ACAT1	Acetyl-CoA acetyltransferase, mitochondrial	0.90
DYNLL1	Dynein light chain 1, cytoplasmic	0.90
GSN	Gelsolin	0.89
IQGAP1	Ras GTPase-activating-like protein IQGAP1	0.88
GPI	Glucose-6-phosphate isomerase	0.86
RPL22	60S ribosomal protein L22	0.83
SOD1	Superoxide dismutase [Cu—Zn]	0.82
PRDX1	Peroxiredoxin-1	0.80
TAGLN2	Transgelin-2	0.78
LAMA5	Laminin subunit alpha-5	0.76
ANXA2	Annexin A2	0.70
RTCB	tRNA-splicing ligase RtcB homolog	0.70
GSTP1	Glutathione S-transferase P	0.70
YWHAG	14-3-3 protein gamma	0.69
MYL6	Myosin light polypeptide 6	0.69
RAB14	Ras-related protein Rab-14	0.68
CALR	Calreticulin	0.67
LMNA	Prelamin-A/C	0.64
NAPA	Alpha-soluble NSF attachment protein	0.64
NCL	Nucleolin	0.64
ARF4	ADP-ribosylation factor 4	0.64
TPI1	Triosephosphate isomerase	0.63
KRT1	Keratin, type II cytoskeletal 1	0.62
ALDOC	Fructose-bisphosphate aldolase C	0.61
CFL1	Cofilin-1	0.61
S100A4	Protein S100-A4	0.59
AP2B1	AP-2 complex subunit beta	0.58
VIM	Vimentin	0.58
COL3A1	Collagen alpha-1(III) chain	0.57
YWHAB	14-3-3 protein beta/alpha	0.57
TGM2	Protein-glutamine gamma-glutamyltransferase 2	0.57
HSP90AA1	Heat shock protein HSP 90-alpha	0.56
KRT9	Keratin, type I cytoskeletal 9	0.56
TMED10	Transmembrane emp24 domain-containing protein 10	0.56
FLOT1	Flotillin-1	0.56
RPL10A	60S ribosomal protein L10a	0.56
GNB2L1	Guanine nucleotide-binding protein subunit beta-2-like 1	0.56
HSPA5	78 kDa glucose-regulated protein	0.55
RPS3A	40S ribosomal protein S3a	0.54
RPS19	40S ribosomal protein S19	0.53
RHOC	Rho-related GTP-binding protein RhoC	0.52
DPYSL2	Dihydropyrimidinase-related protein 2	0.51
SF3B1	Splicing factor 3B subunit 1	0.50
CD44	CD44 antigen	0.49
41889	Septin-7	0.49
CAPN2	Calpain-2 catalytic subunit	0.48
CAV1	Caveolin-1	0.47
RTN4	Reticulon-4	0.46
AHSG	Alpha-2-HS-glycoprotein	0.44
ACTR3	Actin-related protein 3	0.44
AHNAK	Neuroblast differentiation-associated protein AHNAK	0.42
HSP90AB1	Heat shock protein HSP 90-beta	0.42
CAP1	Adenylyl cyclase-associated protein 1	0.40
XRCC6	X-ray repair cross-complementing protein 6	0.40
AP2M1	AP-2 complex subunit mu	0.40
FN1	Fibronectin	0.39
MGST1	Microsomal glutathione S-transferase 1	0.38
VCL	Vinculin	0.38
PFN1	Profilin-1	0.36
FLNA	Filamin-A	0.36
NACA	Nascent polypeptide-associated complex subunit alpha	0.35
ITGB1	Integrin beta-1	0.35
FLNC	Filamin-C	0.35
DBI	Acyl-CoA-binding protein	0.35
IDH1	Isocitrate dehydrogenase [NADP] cytoplasmic	0.34
LGALS1	Galectin-1	0.31
NNMT	Nicotinamide N-methyltransferese	0.30
ASPH	Aspertyl/asparaginyl beta-hydroxylase	0.30
VAPA	Vesicle-associated membrane protein-associated protein A	0.29
41884	Septin-2	0.28
ARHGDIA	Rho GDP-dissociation inhibitor 1	0.26
BSG	Basigin	0.26
THBS1	Thrombospondin-1	0.25
LAMC1	Laminin subunit gamma-1	0.24
PRKDC	DNA-dependent protein kinase catalytic subunit	0.22
S100A6	Protein S100-A6	0.20
SERPINF1	pigment epithelium-derived factor	0.19
DDX1	ATP-dependent RNA helicase DDX1	0.18
TLN1	Talin-1	0.18
PRKCSH	Glucosidase 2 subunit beta	0.18
NDUFV2	NADH dehydrogenase [ubiquinone] flavoprotein2, mitochondrial	0.18
STAT1	Signal transducer and activator of transcription 1-alpha/beta	0.17
RCN1	Reticulocalbin-1	0.17
ADH5	Alcohol dehydragenase class-3	0.16
ARF6	ADP-ribosylation factor 6	0.15
COL12A1	Collagen alpha-1(XII) chain	0.14
PSAP	Proactivator polypeptide	0.13
LRP1	Prolow-density lipoprotein receptor-releted protein 1	0.12
CAT	Catalase	0.12
ANPEP	Aminopeptidase N	0.07
COL1A2	Collagen alpha-2(I) chain	0.07
UGDH	UDP-glucose 6-dehydrogenase	0.06
COL1A1	Collagen alpha-1(I) chain	0.05
HEXB	Beta-hexosaminidase subunit beta	0.03

TABLE 4
Lists of proteins significantly overrepresented in engineered VF mucosa compared
to VFE on scaffold, VFE on scaffold compared to engineered VF mucosa, engineered VF
mucosa compared to VFF in scaffold, and VFF in scaffold compared to engineered VF
mucosa. The cutoff criteria for quantitative protein overrepresentation were fold change >4 and
Benjamini Hochberg-adjusted P < 0.01. Corresponding Volcano plots and heatmaps are
presented in FIG. 7. Proteins are listed in order of descending fold change.
Uniprot
accession		Gene
number	Protein name	symbol	Fold change	Adjusted P-value
Overrepresented in engineered VF mucosa compared to VFE on scaffold
Q92928	Putative Ras-related protein Rab-1C	RAB1C	136.29	1.79E−05
P05141	ADP/ATP translocase 2	SLC25A5	127.20	2.44E−05
P10909	Clusterin	CLU	90.45	1.06E−04
P08134	Rho-related GTP-binding protein RhoC	RHOC	86.81	1.11E−04
Q92688	Acidic leucine-rich nuclear phosphoprotein 32 family member B	ANP32B	72.09	6.66E−05
Q9UBI6	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12	GNG12	56.21	6.69E−05
P46776	60S ribosomal protein L27a	RPL27A	55.56	3.36E−04
P84103	Serine/arginine-rich splicing factor 3	SRSF3	50.11	5.32E−04
P62280	40S ribosomal protein S11	RPS11	48.83	7.13E−05
P10619	Lysosomal protective protein	CTSA	47.59	2.94E−04
P13987	CD59 glycoprotein	CD59	44.45	3.95E−04
O94973	AP-2 complex subunit alpha-2	AP2A2	43.47	3.37E−03
P45974	Ubiquitin carboxyl-terminal hydrolase 5	USP5	43.18	5.22E−04
P62266	40S ribosomal protein S23	RPS23	37.52	1.90E−04
O43143	Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15	DHX15	35.96	1.11E−04
O75533	Splicing factor 3B subunit 1	SF3B1	30.75	9.95E−03
Q9UKK3	Poly [ADP-ribose] polymerase 4	PARP4	27.01	9.73E−05
P61254	60S ribosomal protein L26	RPL26	26.69	3.15E−04
P01026	Complement C3 [Cleaved into: Complement C3 beta chain; Complement C3	C3	24.97	6.50E−04
	alpha chain]
P30046	D-dopachrome decarboxylase	DDT	24.82	3.71E−04
P30740	Leukocyte elastase inhibitor	SERPINB1	23.87	4.39E−03
Q6IBS0	Twinfilin-2	TWF2	23.87	4.46E−03
O75351	Vacuolar protein sorting-associated protein 4B	VPS4B	22.45	2.97E−04
Q9UEY8	Gamma-adducin	ADD3	19.93	1.08E−03
P20073	Annexin A7	ANXA7	19.78	6.89E−04
Q9NYL9	Tropomodulin-3	TMOD3	17.74	1.91E−04
O94855	Protein transport protein Sec24D	SEC24D	15.70	8.45E−04
P36405	ADP-ribosylation factor-like protein 3	ARL3	15.70	8.65E−04
P55036	26S proteasome non-ATPase regulatory subunit 4	PSMD4	13.18	9.95E−03
P08648	Integrin alpha-5	ITGA5	11.31	8.87E−03
Q9P0L0	Vesicle-associated membrane protein-associated protein A	VAPA	10.99	2.97E−03
P21291	Cysteine and glycine-rich protein 1	CSRP1	8.79	5.01E−03
P62491	Ras-related protein Rab-11A	RAB11A	6.75	3.33E−04
Overrepresented in VFE on scaffold compared to engineered mucosa
P05539	Collagen alpha-1(II) chain	COL2A1	418.83	1.06E−04
P62820	Ras-related protein Rab-1A	RAB1A	127.00	4.58E−05
P08962	CD63 antigen	CD63	51.40	3.73E−04
P02770	Serum albumin	ALB	49.99	1.62E−05
P43235	Cathepsin K	CTSK	49.08	1.27E−03
P35754	Glutaredoxin-1	GLRX	44.94	1.02E−04
P13693	Translationally-controlled tumor protein	TPT1	44.56	1.85E−04
P01889	HLA class I histocompatibility antigen, B-7 alpha chain	HLA-B	42.11	1.15E−05
P20340	Ras-related protein Rab-6A	RAB6A	37.88	9.66E−05
P62753	40S ribosomal protein S6	RPS6	35.28	5.16E−03
P02795	Metaliothionein-2	MT2A	31.19	9.90E−03
P37235	Hippocalcin-like protein 1	HPCAL1	30.97	1.65E−05
P23141	Liver carboxylesterase 1	CES1	28.75	2.32E−05
P63173	60S ribosomal protein L38	RPL38	28.37	1.99E−04
P48681	Nestin	NES	27.71	7.33E−04
Q92945	Far upstream element-binding protein 2	KHSRP	27.27	3.28E−03
P62899	60S ribosomal protein L31	RPL31	25.86	4.10E−03
P62847	40S ribosomal protein S24	RPS24	25.55	6.95E−03
P61163	Alpha-centractin	ACTR1A	24.13	1.53E−03
O95292	Vesicle-associated membrane protein-associated protein B/C	VAPB	23.47	1.76E−04
P02649	Apolipoprotein E	APOE	23.25	5.35E−04
Q9Y5X1	Sorting nexin-9	SNX9	21.84	1.35E−03
P14406	Cytochrome c oxidase subunit 7A2, mitochondrial	COX7A2	21.84	1.33E−03
Q9Y646	Carboxypeptidase Q	CPQ	20.21	9.72E−03
P22307	Non-specific lipid-transfer protein	SCP2	19.24	1.92E−05
P28799	Granulins	GRN	19.02	1.26E−03
Q1KMD3	Heterogenous nuclear ribonucleoprotein U-like protein 2	HNRNPUL2	17.23	1.26E−03
P27706	CAD protein [Includes: Glutamine-dependent carbamoyl-phospnate synthase	CAD	15.75	6.95E−03
P62913	60S ribosomal protein L11	RPL11	13.37	9.90E−05
Q16718	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5	NDUFA5	13.37	1.07E−04
P67812	Signal peptidase complex catalytic subunit SEC11A	SEC11A	11.74	3.26E−04
P49821	NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial	NDUFV1	11.74	3.07E−04
Q15063	Periostin	POSTN	11.74	3.16E−04
Q9BTV4	Transmembrane protein 43	TMEM43	11.14	7.27E−03
O60749	Sorting nexin-2	SNX2	9.51	3.33E−03
O00754	Lysosomal alpha-mannosidase	MAN2B1	5.87	8.48E−04
Overrepresented in engineered VF mucosa compared to VFF in scaffold
P42330	Aldo-keto reductase family 1 member C3	AKR1C3	228.80	9.75E−05
P35556	Fibrillin-2	FBN2	213.61	2.29E−04
P98095	Fibulin-2	FBLN2	176.02	6.98E−05
P39687	Acidic leucine-rich nuclear phosphoprotein 32 family member A	ANP32A	94.87	1.37E−04
P27105	Erythrocyle band 7 integral membrane protein	STOM	93.36	1.59E−04
Q9Y6C2	EMILIN-1	EMILIN1	92.41	2.15E−04
P61586	Transforming protein RhoA	RHOA	89.42	1.18E−04
P06753	Tropomyosin alpha-3 chain	TPM3	84.67	1.10E−04
P13674	Prolyl 4-hydroxylase subunit alpha-1	P4HA1	82.35	9.22E−05
P42785	Lysosomal Pro-X carboxypeptidase	PRCP	72.67	8.12E−05
P00325	Alcohol dehydrogenase 1B	ADH1B	69.66	5.62E−03
P20700	Lamin-B1	LMNB1	61.16	1.60E−04
P60866	40S ribosomal protein S20	RPS20	58.85	8.84E−05
P62750	60S ribosomal protein L23a	RPL23A	58.69	1.21E−04
P51688	N-sulphoglucosamine sulphohydrolase	SGSH	57.39	3.73E−04
P46926	Glycosamine-6-phosphate isomerase 1	GNPDA1	56.55	9.99E−05
P01893	Putative HLA class I histocompatibility antigen, alpha chain H	HLA-H	55.40	2.01E−04
P00966	Argininosuccinate synthase	ASS1	53.74	2.19E−04
P55795	Heterogeneous nuclear ribonucleoprotein H2	HNRNPH2	53.42	1.10E−04
Q8NHP8	Putative phospholipase B-like 2	PLBD2	52.95	7.91E−04
P27635	60S ribosomal protein L10	RPL10	52.12	1.50E−04
P24752	Acetyl-CoA acetyltransferase, mitochondrial	ACAT1	47.33	3.09E−03
P23142	Fibulin-1	FBLN1	47.16	7.94E−03
P13987	CD59 glycoprotein	CD59	46.53	2.86E−04
Q16363	Laminin subunit alpha-4	LAMA4	46.51	1.41E−04
Q9BS26	Endoplasmic reticulum resident protein 44	ERP44	46.01	4.15E−04
O14773	Tripeptidyl-peptidase 1	TPP1	45.53	1.23E−03
O94973	AP-2 complex subunit alpha-2	AP2A2	45.52	1.82E−03
P45974	Ubiquitin carboxyl-terminal hydrolase 5	USP5	45.21	3.60E−04
Q9Y3I0	tRNA-splicing ligase RtcB homolog	C22orf28	44.55	1.26E−04
P42224	Signal transducer and activator of transcription 1-alpha/beta	STAT1	43.88	3.65E−04
P25789	Proteasome subunit alpha type-4	PSMA4	42.59	7.01E−04
P30084	Enoyl-CoA hydratase, mitochondrial	ECHS1	42.57	2.27E−04
P19623	Spermidine synthase	SRM	40.58	5.78E−03
Q14258	E3 ubiquitin/ISG15 ligase TRIM25	TRIM25	40.28	1.94E−04
P20618	Proteasome subunit beta type-1	PSMB1	39.78	2.17E−04
P62266	40S ribosomal protein S23	RPS23	39.28	1.63E−04
Q9UBQ7	Glyoxylate reduclase/hydroxypyruvate reductase	GRHPR	39.12	3.17E−04
P36543	V-type proton ATPase subunit E1	ATP6V1E1	37.82	3.71E−04
Q9Y3U8	60S ribosomal protein L36	RPL36	37.80	5.88E−04
P40261	Nicotinamide N-methyltransferase	NNMT	37.66	1.69E−03
O43143	Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15	DHX15	37.64	1.16E−04
Q6NUM9	All-trans-retinol 13,14-reduclase	RETSAT	36.81	8.06E−04
P13473	Lysosome-associated membrane glycoprotein 2	LAMP2	35.68	4.10E−04
P07711	Cathepsin L1	CTSL1	35.18	2.14E−04
P19404	NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial	NDUFV2	33.55	7.09E−04
Q9NR45	Sialic acid synthase	NANS	33.38	1.35E−03
Q15075	Early endosome antigen I	EEA1	32.53	1.15E−03
Q92597	Protein NDRG1	NDRG1	32.39	1.16E−03
Q08380	Galectin-3-binding protein	LGALS3BP	32.37	4.41E−04
O75533	Splicing factor 3B subunit 1	SF3B1	32.20	5.45E−03
P46439	Glutathione S-transferase Mu 5	GSTM5	32.20	5.40E−03
P04062	Glucosylceramidase	GBA	31.91	8.42E−03
Q12906	Interleukin enhancer-binding factor 3	ILF3	31.59	7.21E−03
Q8IZP2	Putative protein FAM10A4	ST13P4	30.91	2.97E−04
P04179	Superoxide dismutase [Mn], mitochondrial	SOD2	30.89	6.68E−03
Q5T9L3	Protein wntless homolog	WLS	30.57	1.51E−04
P07954	Fumarate hydratase, mitochondrial	FH	30.57	1.57E−04
P27487	Dipeptidyl peptidase 4	DPP4	30.41	8.97E−05
Q9UIJ7	GTP:AMP phosphotransferase AK3, mitochondrial	AK3	30.41	8.37E−05
O14880	Microsomal glutathione S-transferase 3	MGST3	29.42	3.18E−03
P35555	Fibrillin-1	FBN1	28.46	7.93E−03
P10768	S-formylglutathione hydrolase	ESD	28.44	7.00E−04
Q9UKK3	Poly [ADP-ribose] polymerase 4	PARP4	28.28	1.01E−04
O95747	Serine/threonine-protein kinase OSR1	OXSR1	28.28	9.63E−05
P61254	60S ribosomal protein L26	RPL26	27.94	2.27E−04
O43491	Band 4.1-like protein 2	EPB41L2	27.78	1.51E−04
O15145	Actin-related protein 2/3 complex subunit 3	ARPC3	27.78	1.56E−04
O43615	Mitochondrial import inner membrane translocase subunit TIM44	TIMM44	26.48	1.47E−03
Q9Y371	Endophilin-B1	SH3GLB1	25.64	6.99E−05
Q32P28	Prolyl 3-hydroxylase 1	LEPRE1	25.64	7.38E−05
P22087	rRNA 2′-O-methyltransferase fibrilliarin	FBL	25.64	7.81E−05
P07942	Laminin subunit beta-1	LAMB1	25.30	1.46E−03
P00403	Cytochrome c oxidase subunit 2	MT-CO2	24.98	2.46E−03
P30740	Leukocyte elastase inhibitor	SERPINB1	24.98	2.48E−03
P28161	Glutathione S-transferase Mu 2	GSTM2	24.34	5.12E−03
P49589	Cysteine-tRNA ligase, cytoplasmic	CARS	23.84	5.53E−04
Q16643	Drebrin	DBN1	23.50	2.19E−04
Q13263	Transcription intermediary factor 1-beta	TRIM28	23.50	2.24E−04
O75351	Vacuolar protein sorting-associated protein 4B	VPS4B	23.50	2.34E−04
P14868	Aspartate--tRNA ligase, cytoplasmic	DARS	23.50	2.29E−04
Q02543	60S ribosomal protein L18a	RPL18A	23.34	1.18E−04
P38606	V-type proton ATPase catalytic subunit A	ATP6V1A	23.01	7.46E−04
O00151	PDZ and LIM domain protein 1	PDLIM1	22.85	1.23E−03
P06756	Integrin alpha-V	ITGAV	22.65	1.25E−03
Q13228	Selenium-binding protein 1	SELENBP1	22.04	7.51E−03
Q13561	Dynactin subunit 2	DCTN2	21.70	2.75E−03
P43490	Nicotinamide phosphoribosyltransferase	NAMPT	21.54	2.15E−03
P62714	Serine/threonine-protein phosphatase 2A calalytic subunit beta isoform	PPP2CB	21.21	1.21E−04
O95336	6-phosphogluconolactonase	PGLS	21.05	1.16E−03
Q14112	Nidogen-2	NID2	20.87	7.16E−04
Q9Y4L1	Hypoxia up-regulated protein 1	HYOU1	19.41	4.46E−03
P80303	Nucleobindin-2	NUCB2	18.91	6.94E−04
P0CW22	40S ribosomal protein S17-like	RPS17L	18.57	1.82E−04
Q02818	Nucleobindin-1	NUCB1	18.07	8.85E−03
P02461	Collagen alpha-1(III) chain	COL3A1	17.57	4.94E−03
P53007	Tricarboxylate transport protein, mitochondrial	SLC25A1	16.77	1.45E−03
P05091	Aldehyde dehydrogenase, mitochondrial	ALDH2	16.44	5.95E−04
Q9HC38	Glyoxalase domain-containing protein 4	GLOD4	16.44	5.86E−04
P36405	ADP-ribosylation factor-like protein 3	ARL3	16.44	6.04E−04
P99999	Cytochrome c	CYCS	16.28	2.54E−04
P46063	ATP-dependent DNA helicase Q1	RECQL	16.28	2.59E−04
P42765	3-ketoacyl-CoA thiolase, mitochondrial	ACAA2	16.28	2.64E−04
P54727	UV excision repair protein RAD23 homolog B	RAD23B	15.94	6.16E−03
O75955	Flotillin-1	FLOT1	15.78	8.61E−03
Q16891	Mitochondrial inner membrane protein	IMMT	14.14	1.53E−04
Q9P2J5	Leucine--tRNA ligase, cyloplasmic	LARS	14.14	1.58E−04
P10515	Dihydrolipoyllysine-residue acetyltransferase component of pyruvate	DLAT	13.80	5.37E−03
	dehydrogenase complex, mitochondrial
P55735	Protein SEC13 homolog	SEC13	13.80	5.41E−03
Q02218	2-oxoglutarate dehydrogenase, mitochondrial	OGDH	13.80	5.45E−03
P55036	26S proteasome non-ATPase regulatory subunit 4	PSMD4	13.80	5.50E−03
P54578	Ubiquitin carboxyl-terminal hydrolase 14	USP14	11.84	4.97E−03
Q12884	Seprase	FAP	11.84	4.93E−03
Q9P0L0	Vesicle-associated membrane protein-associated protein A	VAPA	11.50	1.66E−03
O15230	Laminin subunit alpha-5	LAMA5	9.70	9.44E−03
Q6IAA8	Regulator complex protein LAMTOR1	LAMTOR1	9.70	9.51E−03
Q96FQ6	Protein S100-A16	S100A16	9.70	9.57E−03
P27701	CD82 antigen	CD82	9.70	9.63E−03
P63220	40S ribosomal protein S21	RPS21	9.37	5.15E−03
Q6P2Q9	Pre-mRNA-processing-splicing factor 8	PRPF8	9.21	2.93E−03
Q9ULZ3	Apoptosis-associated speck-like protein containing a CARD	PYCARD	9.21	2.90E−03
P82491	Ras-related protein Rab-11A	RAB11A	7.07	3.20E−04
Overrepresented in VFF in scaffold compared to engineered VF mucosa
P05539	Collagen alpha-1(II) chain	COL2A1	333.66	1.19E−04
P02770	Serum albumin	ALB	68.56	2.08E−04
P50903	Protein S100-A10	S100A10	49.96	4.11E−04
P01024	Complement C3	C3	45.51	3.10E−03
P02774	Vitamin D-binding protein	GC	42.27	2.93E−06
P62857	40S ribosomal protein S28	RPS28	41.71	8.10E−04
P02788	Lactotransferrin	LTF	40.99	6.76E−04
P13693	Translationally-controlled tumor protein	TPT1	39.35	3.21E−04
P01008	Antithrombin-III	SERPINC1	22.39	5.52E−03
P30493	HLA class I histocompatibility antigen, B-55 alpha chain	HLA-B	21.48	1.76E−03
P62753	40S ribosomal protein S6	RPS6	21.05	9.25E−04
P62917	60S ribosomal protein L8	RPL8	13.95	6.92E−03
P35637	RNA-binding protein FUS	FUS	13.65	3.67E−03
P49458	Signal recognition particle 9 kDa protein	SRP9	13.06	7.08E−04
O15143	Actin-related protein 2/3 complex subunit 1B	ARPC1B	7.40	5.74E−03
P67809	Nuclease-sensitive element-binding protein 1	YBX1	7.40	5.79E−03
Q9BR76	Coronin-1B	CORO1B	7.40	5.83E−03

TABLE 5
Gene ontology biological process terms enriched in the protein set exclusive to
engineered VF mucosa or overrepresented in engineered mucosa compared to both VFF in
scaffold and VFE on scaffold. The cutoff criteria for quantitative protein enrichment
overrepresentation were fold change >4 and Benjamini Hochberg-adjusted P < 0.01. Enriched
ontology terms were identified using the BiNGO enrichment and REViGO term redundancy
algorithms. A subset of these biological process terms are presented in FIG. 6D. Terms are
listed in order of ascending Benjamini Hochberg-adjusted P-value.
Biological process term	Description	Adjusted P-value
GO: 0044265	cellular macromolecule catabolic process	1.36E−05
GO: 0008104	protein localization	2.30E−05
GO: 0071840	cellular component organization or biogenesis	3.97E−05
GO: 0016071	mRNA metabolic process	1.29E−04
GO: 0034641	cellular nitrogen compound metabolic process	2.53E−04
GO: 0006807	nitrogen compound metabolic process	4.12E−04
GO: 0022411	cellular component disassembly	7.32E−04
GO: 0086004	regulation of cardiac muscle cell contraction	8.91E−04
GO: 0006413	translational initiation	1.41E−03
GO: 0044281	small molecule metabolic process	1.47E−03
GO: 1901360	organic cyclic compound metabolic process	1.56E−03
GO: 0007044	cell-substrate junction assembly	3.03E−03
GO: 0044710	single-organism metabolic process	3.31E−03
GO: 0046483	heterocycle metabolic process	5.18E−03
GO: 0008544	epidermis development	5.27E−03
GO: 0006725	cellular aromatic compound metabolic process	5.27E−03
GO: 0034332	adherens junction organization	7.35E−03
GO: 0071704	organic substance metabolic process	7.55E−03
GO: 0006577	amino-acid betaine metabolic process	8.58E−03
GO: 0034329	cell junction assembly	8.72E−03
GO: 0044349	DNA excision	9.22E−03
GO: 0030162	regulation of proteolysis	9.35E−03

TABLE 6
Antibodies and isotype controls used for flow cytometry.
Item	Clone	Fluorochrome	Dilution	Manufacturer	Catalog number
Antibodies
rabbit anti-human P4H-β	polyclonal	FITC	1:50	Bioss	bs-5090R
mouse anti-human CD90	5E10	PE-Cy7	1:20	BD Biosciences	561558
mouse anti-human keratin 14	LL002	FITC	1:10	Abcam	ab77684
mouse anti-human pan-keratin	C11	Alexa 647	1:50	Cell Signaling Technology	4528
mouse anti-human CD227	16A	PE	1:20	BioLegend	355603
mouse anti-human keratin 19	RCK108	PerCP	1:5	Santa Cruz Biotechnology	sc-53003
mouse anti-human HLA-ABC	W6/32	Alexa 488	1:20	BioLengend	311415
mouse anti-human HLA-DR	LN3	PE	1:20	eBioscience	12-9956
mouse anti-human PD-L1 (CD274)	29E.2A3	APC	1:20	BioLegend	329708
mouse anti-human PD-L2 (CD273)	MIH18	APC	1:20	BioLegend	345507
mouse anti-human CD80	L307.4	FITC	1:20	BD Biosciences	557226
mouse anti-human CD86	IT2.2	PE	1:20	BioLegend	305406
mouse anti-human pan-CD45	HI30	APC	1:20	BD Biosciences	555485
rat anti-mouse pan-CD45	30F11	FITC	1:20	BD Biosciences	553080
Isotype controls
rabbit IgG	EPR25A	none	1:100	Abcam	ab172730
mouse IgG1	ICIGG1	none	1:200	Abcam	ab91353
	MOPC-21	APC	1:20	BD Biosciences	400121
	MOPC-21	FITC	1:20	BD Biosciences	400107
	MOPC-21	PE	1:20	BD Biosciences	400111
mouse IgG2b	MPC-11	APC	1:20	BioLegend	400321
	MPC-11	PE	1:20	BioLegend	400314
rat IgG2b	A95-1	FITC	1:20	BD Biosciences	553988

TABLE 7
Antibodies used for immunocytochemistry and immunohistochemistry.
Item	Clone	Dilution	Manufacturer	Catalog number	Positive control
Primary antibodies
rabbit anti-human β-cadherin	polyclonal	1:100	Santa Cruz Biotechnology	sc-7870	vocal fold
mouse anti-human P4H-β	3-2B12	1:250	Chemicon	MAB2701	vocal fold
goat anti-human α-actin 2	polyclonal	1:100	Thermo Scientific	PA5-18292	vocal fold
mouse anti-human collagen, type IV	COL-94	1:100	Sigma-Aldrich	C1926	vocal fold
rabbit anti-human laminin 5	polyclonal	1:200	Abcam	ab14509	vocal fold
mouse anti-human keratin 5	XM26	1:100	Santa Cruz Biotechnology	sc-58732	vocal fold
mouse anti-human junction plakoglobin	4C12	1:200	Abcam	ab119908	vocal fold
mouse anti-human mitochondria	113-1	1:200	Millipore	MAB1273	thymus
rabbit anti-human forkhead box P3	polyclonal	1:300	Abcam	ab10563	thymus
rabbit anti-human CD8	SP16	1:100	Biocare Medical	CRM311	thymus
rabbit anti-human CD4	EPR6855	1:200	Abcam	ab133616	thymus
mouse anti-human CD45	2B11; PD7/26	1:100	Innovex	MAB321C	thymus
Secondary antibodiesa
Alexa 488-conjugated donkey anti-mouse IgG		1:200	Life Technologies	A-21202
Alexa 594-conjugated donkey anti-mouse IgG		1:200	Life Technologies	A-21203
Alexa 488-conjugated donkey anti-rabbit IgG		1:200	Life Technologies	A-21206
Alexa 594-conjugated donkey anti-rabbit IgG		1:200	Life Technologies	A-21207
Alexa 594-conjugated donkey anti-goat IgG		1:200	Life Technologies	A-11058
aSecondary antibodies used for fluorescent detection are listed here; HRP-based detection is described in the Example Materials and Methods.

Claims

1. An engineered vocal fold mucosa comprising: (a) an engineered non-vascularized lamina propria comprising a scaffold comprising polymerized collagen populated by a plurality of human vocal fold fibroblasts (VFFs); and(b) an engineered stratified squamous epithelium in contact with the engineered non-vascularized lamina propria comprising a plurality of human vocal fold epithelial cells (VFEs);wherein the engineered vocal fold mucosa is capable of exhibiting the vibratory function and acoustic output of a native vocal fold mucosa.

2. The engineered vocal fold mucosa of claim 1, wherein the polymerized collagen is polymerized collagen, type I.

3. The engineered vocal fold mucosa of claim 1, wherein the density of the VFFs populating the engineered non-vascularized lamina propria is from 100-300 cells/mm2.

4. The engineered vocal fold mucosa of claim 3, wherein the density of the VFFs populating the engineered non-vascularized lamina propria is from 130-270 cells/mm2.

5. The engineered vocal fold mucosa of claim 1, wherein the engineered stratified squamous epithelium is between 30 and 70 um thick.

6. The engineered vocal fold mucosa of claim 1, wherein one or more VFEs at both the basal and epithelial surface regions of the engineered stratified squamous epithelium express the basement membrane marker collagen, type IV.

7. The engineered vocal fold mucosa of claim 1, wherein the engineered stratified squamous epithelium comprises one or more Keratin 5+ VFEs, and wherein the basal region of the engineered stratified squamous epithelium does not have a higher percentage of Keratin 5+ VFEs than the stratified squamous epithelium as a whole.

8. The engineered vocal fold mucosa of claim 1, further comprising one or more of the proteins listed in Table 1 as being present in the engineered VF mucosa.

9. The engineered vocal fold mucosa of claim 8, wherein at least one of the one or more of the proteins listed in Table 1 as being present in the engineered VF mucosa is a protein that is not present in native VF mucosa.

10. An engineered vocal fold mucosa according to claim 1 for use in treating voice impairment caused by vocal fold fibrosis or vocal fold mucosal tissue damage or loss.

11. An engineered vocal fold mucosa according to claim 1 for use in manufacturing a composition for treating voice impairment caused by vocal fold fibrosis or vocal fold mucosal tissue damage or loss.

12. A method of treating voice impairment caused by vocal fold fibrosis or vocal fold mucosal tissue damage or loss, comprising implanting an engineered vocal fold mucosa according to claim 1 into the larynx of a subject in need thereof, whereby the voice impairment is reduced.

13. A method of making an engineered vocal fold mucosa, comprising: (a) culturing a plurality of human vocal fold fibroblasts (VFFs) within a scaffold comprising polymerized collagen; and(b) culturing a plurality of human vocal fold epithelial cells (VFEs) on the scaffold surface;thereby forming an engineered vocal fold mucosa comprising an engineered stratified squamous epithelium in contact with an engineered non-vascularized lamina propria comprising a scaffold comprising polymerized collagen and populated by a plurality of human VFFs.

14. The method of claim 13, wherein the polymerized collagen is polymerized collagen, type I.

15. The method of claim 13, wherein the step of culturing the plurality of human VFEs on the scaffold surface is first performed within an epithelial cell-oriented medium in which the human VFEs are immersed.

16. The method of claim 15, wherein the step of culturing the plurality of human VFEs on the scaffold surface is later performed at an air-liquid interface on the scaffold surface, wherein the scaffold comprises a composition comprising both a fibroblast-oriented medium and an epithelial cell-oriented medium, and whereby the human VFEs stratify to form a squamous epithelium layer.

17. The method of claim 16, wherein the human VFEs are cultured for 1 to 3 days within the epithelial cell-oriented medium in which the human VFEs are immersed.

18. The method of claim 16, wherein the human VFEs are cultured for 5 to 50 days at the air-liquid interface on the scaffold surface.

19. The method of claim 13, wherein the step of culturing the plurality of human VFFs within the scaffold is first performed when the scaffold comprises a fibroblast-oriented medium, and later performed when the scaffold comprises a composition comprising both a fibroblast-oriented medium and an epithelial cell-oriented medium.

20. The method of claim 13, wherein prior to culturing the human VFFs within the scaffold, the human VFFs are seeded into the scaffold at a density of between 5×104 and 1×106 VFF/mL.

21. The method of claim 13, wherein the human VFFs are obtained by: (a) seeding cells obtained from a human vocal fold mucosa onto an extracellular matrix (ECM)-coated surface, whereby some of the cells adhere to the ECM-coated surface and some of the cells remain free-floating and non-adherent; and(b) culturing the cells that adhere to the ECM-coated surface in a fibroblast-oriented medium, resulting in a substantially pure subpopulation of human VFFs.

22. The method of claim 21, wherein human VFEs are obtained by the further steps of: (c) separately culturing the non-adherent cells in an epithelial cell-oriented medium, whereby the non-adherent cells become adherent; and(d) incubating the now-adherent cells in a composition comprising trypsin and removing one or more initially detached cells.

23. The method of claim 22, wherein the remaining adherent cells are then detached and passaged into a separate epithelial cell-oriented medium, whereby the cells again become adherent.

24. The method of claim 23, wherein the passaged cells are again incubated in a composition comprising trypsin, and wherein one or more initially detached cells are again removed, resulting in a substantially pure subpopulation of human VFEs.

25. The method of claim 22, wherein the culturing steps (b), (c), or both further comprise passaging the cultured cells at least twice.

26. The method of claim 25, wherein the human VFFs, the human VFEs, or both are obtained from the second passage, the third passage, the fourth passage, the fifth passage, the sixth passage, or any combination thereof.

27. The method of claim 21, wherein the cells are obtained from the human vocal fold mucosa by: (a) mincing the human vocal fold mucosa; and(b) enzymatically digesting the minced mucosa to release the cells from the extracellular matrix (ECM).

28. A method of separating cells obtained from human vocal fold mucosa into a substantially pure human VFF subpopulation, comprising: (a) seeding cells obtained from a human vocal fold mucosa onto an extracellular matrix (ECM)-coated surface, whereby some of the cells adhere to the ECM-coated surface and some of the cells remain free-floating and non-adherent; and(b) culturing the cells that adhere to the ECM-coated surface in fibroblast-oriented medium, resulting in a substantially pure subpopulation of human VFFs.

29. The method of claim 28, further wherein the cells are further separated into a VFE subpopulation by: (c) separately culturing the non-adherent cells in an epithelial cell-oriented medium, whereby the non-adherent cells become adherent; and(d) incubating the now-adherent cells in a composition comprising trypsin and removing one or more initially detached cells.

30. The method of claim 29, wherein the remaining adherent cells are detached and passaged into a separate epithelial cell-oriented medium, whereby the cells again become adherent.

31. The method of claim 30, wherein the passaged cells are again incubated in a composition comprising trypsin, and wherein one or more initially detached cells are again removed, resulting in a substantially pure subpopulation of human VFEs.

32. The method of claim 29, wherein the cells are obtained from the human vocal fold mucosa by: (a) mincing the human vocal fold mucosa; and(b) enzymatically digesting the minced mucosa to release the cells from the extracellular matrix (ECM).

33. An engineered vocal fold mucosa as made by performing the method of claim 13.