Patent Application
20240292843
Embodiments of the present disclosure relate to a mixture for removing biofilm from surfaces. Some relate to a mixture for removing biofilm from the biopsy channel of an endoscope.
Biofilms include a number of microorganisms within an extracellular matrix, and can form on a wide variety of surfaces. For instance, mature biofilms can form on the inner walls of a biopsy channel of an endoscope.
Biofilms can contain a wide spectrum of pathogenic microorganisms including gram-positive bacteria, gram-negative bacteria, and fungi. Bacteria within a biofilm can be 10 to 1000 times more difficult to remove than planktonic bacteria. Due to the risk of infection, it is highly desirable to remove biofilms from surfaces, especially in environments such as hospitals or food preparation areas. However, present means of removing biofilm can be slow, damage the surface, and/or inadequately remove the whole spectrum of pathogenic microorganisms from the surface.
The term “at least” used in this specification is defined as greater than or equal to (i.e., ≥). The term “up to” used in this specification is defined as a smaller value than or equal to (i.e., ≤).
According to various, but not necessarily all, embodiments there is provided a cleaning mixture for removing biofilm from surfaces, the mixture comprising: silver particles and/or particles surface modified with silver; manganese dioxide particles and/or particles surface modified with manganese oxide; and water.
The mixture may further comprise hydrogen peroxide. Possibly, the mixture comprises up to 5 wt. % hydrogen peroxide. Possibly, the mixture comprises up to 2 wt. % hydrogen peroxide.
The mixture may comprise silver particles. Possibly, the silver particles are present in the mixture at a concentration of from 0.1 mg/mL to 1 mg/mL. Possibly, the silver particles are present in the mixture at a concentration of from 0.2 mg/ml to 0.5 mg/mL.
Possibly, the silver particles have a hydrodynamic diameter of up to 1 μm. Possibly, the silver particles have a hydrodynamic diameter of up to 500 nm. Possibly, the silver particles have a hydrodynamic diameter of up to 200 nm.
The mixture may comprise manganese dioxide particles. Possibly, the manganese dioxide particles are present in the mixture at a concentration of from 0.0002 mg/mL to 0.005 mg/mL. Possibly, the manganese dioxide particles are present in the mixture at a concentration of from 0.0005 mg/mL to 0.003 mg/mL.
Possibly, the manganese dioxide particles have a hydrodynamic diameter of up to 1 μm. Possibly, the manganese dioxide particles have a hydrodynamic diameter of up to 500 nm.
The mixture may further comprise a capping agent. The capping agent may be a water-soluble polymer capping agent. The polymer capping agent may be polyvinylpyrrolidone.
The mixture may comprise silver particles and manganese dioxide particles.
The mixture may comprise particles surface modified with silver and particles surface modified with manganese oxide. Possibly, at least some of the particles surface modified with silver and at least some of the particles surface modified with manganese oxide are particles surface modified with both silver and manganese oxide. The particles surface modified with both silver and manganese oxide may be zeolite particles surface modified with both silver and manganese oxide.
According to various, but not necessarily all, embodiments there is provided a cleaning mixture for removing biofilm from surfaces, the mixture consisting of: silver particles and/or particles surface modified with silver; manganese dioxide particles and/or particles surface modified with manganese oxide; and water.
The mixture may further consist of hydrogen peroxide.
According to various, but not necessarily all, embodiments there is provided a cleaning mixture for removing biofilm from surfaces, the mixture comprising: silver containing particles; manganese oxide containing particles; and water.
The surface of the silver containing particles may comprise silver. The surface of the manganese oxide containing particles may comprise manganese oxide.
The mixture may further comprise hydrogen peroxide. Possibly, the mixture comprises up to 5 wt. % hydrogen peroxide. Possibly, the mixture comprises up to 2 wt. % hydrogen peroxide.
According to various, but not necessarily all, embodiments there is provided a method of removing biofilm from a surface, the method comprising: applying the cleaning mixture of any of the preceding paragraphs to the surface.
Hydrogen peroxide may be added to the mixture up to 5 minutes before applying the cleaning mixture to the surface.
The cleaning mixture may be applied to the surface for up to 15 minutes.
The surface may be the inner surface of a tube. The cleaning mixture may be applied by passing the mixture through the tube. The tube may be the biopsy channel of an endoscope.
The method may further comprise applying an enzymatic solution to the surface. The method may comprise passing the enzymatic solution through the biopsy channel prior to passing the cleaning mixture through the biopsy channel.
The method may further comprise rinsing the surface with water after the application of the cleaning mixture to the surface.
According to various, but not necessarily all, embodiments there is provided a system for removing biofilm from the biopsy channel of an endoscope, the system comprising: a reservoir containing the cleaning mixture of any of the preceding paragraphs; and an inlet, which is arranged to direct fluid into an internal channel of the endoscope.
The system may further include a bath for locating the endoscope. The system may include one or more inlets into the bath. The system may include a drain for draining liquid from the bath.
Examples of the disclosure also provide a method of producing manganese dioxide particles, comprising: mixing a manganese (II) salt and an acetate salt with a capping agent in water to form a first solution; and adding an oxidizing agent to the first solution.
The method may be carried out at room temperature.
The capping agent may be a water-soluble polymer capping agent. The polymer capping agent may be polyvinylpyrrolidone.
The manganese (II) salt may be manganese acetate. The acetate salt may be manganese acetate. The oxidizing agent may be potassium permanganate. The ratio of the moles of manganese acetate in the first solution to the moles of potassium permanganate added to the first solution may be from 1:1 (mol Mn(CH3COO)2:mol KMnO4) to 2:1 (mol Mn(CH3COO)2:mol KMnO4).
Tannic acid may also be mixed with the manganese acetate and capping agent in water, prior to the addition of the potassium permanganate.
The solution may be stirred prior to and/or after the addition of the potassium permanganate. The potassium permanganate may be added in a dropwise fashion.
According to various, but not necessarily all, embodiments there is provided examples as claimed in the appended claims.
Some examples will now be described with reference to the accompanying drawings in which:
Examples of the disclosure provide a cleaning mixture for removing biofilm from surfaces, the mixture comprising:
In a first example of the cleaning mixture for removing biofilm from surfaces, the mixture comprises silver particles, manganese dioxide particles, and water.
The first example cleaning mixture for removing biofilm from surfaces disclosed herein includes silver particles. In some examples, the silver particles have a hydrodynamic diameter of up to 100 μm, as measured using dynamic light scattering. The hydrodynamic diameter is the diameter of a hypothetical hard sphere that diffuses with the same speed as the particle being measured. The hydrodynamic diameters described herein are as measured using dynamic light scattering.
In some examples, the silver particles have a hydrodynamic diameter of up to 50 μm, or up to 20 μm. Preferably, the silver particles have a hydrodynamic diameter of up to 1 μm, up to 500 nm, up to 200 nm, or up to 150 nm. Particles with smaller sizes have been found to be more effective for use in a cleaning solution, as the particles can remain dispersed for longer time periods. The silver particles may have a hydrodynamic diameter of 10 nm to 200 nm. Most preferably, the silver particles have a hydrodynamic diameter of 50 nm to 150 nm (such as 127 nm).
The cleaning mixture for removing biofilm from surfaces disclosed herein further includes manganese dioxide particles. In some examples, the manganese dioxide particles have a hydrodynamic diameter of up to 100 μm, as measured using dynamic light scattering. The hydrodynamic diameter is the diameter of a hypothetical hard sphere that diffuses with the same speed as the particle being measured. The hydrodynamic diameters described herein are as measured using dynamic light scattering.
In some examples, the manganese dioxide particles have a hydrodynamic diameter of up to 50 μm, or up to 20 μm. Preferably, the manganese dioxide particles have a hydrodynamic diameter of up to 1 μm, up to 500 nm, or up to 300 nm. Particles with smaller sizes have been found to be more effective for use in a cleaning solution, as the particles can remain dispersed for longer time periods. The manganese dioxide particles may have a hydrodynamic diameter of 10 nm to 300 nm. Most preferably, the manganese dioxide particles have a hydrodynamic diameter of 150 nm to 250 nm (such as 206 nm).
In some examples, the silver particles are present in the mixture at a concentration of at least 0.01 mg/mL, at least 0.05 mg/mL, at least 0.1 mg/mL, at least 0.2 mg/mL, or at least 0.3 mg/mL. In some examples, the silver particles are present in the mixture at a concentration of up to 10 mg/mL, up to 5 mg/mL, up to 1 mg/ml up to 0.7 mg/mL, up to 0.5 mg/mL, or up to 0.4 mg/mL. Preferably, the silver particles are present in the mixture at a concentration of from 0.1 mg/mL to 1 mg/mL. Most preferably, the silver particles are present in the mixture at a concentration of from 0.2 mg/ml to 0.5 mg/mL, such as 0.326 mg/mL.
In some examples, the manganese dioxide particles are present in the mixture at a concentration of at least 0.0001 mg/mL, at least 0.0003 mg/mL, at least 0.0005 mg/mL, or at least 0.0008 mg/mL. In some examples, the manganese dioxide particles are present in the mixture at a concentration of up to 1 mg/mL, up to 0.1 mg/mL, up to 0.01 mg/mL, up to 0.007 mg/mL, up to 0.005 mg/mL, up to 0.003 mg/mL, or up to 0.002 mg/mL. Preferably, the manganese dioxide particles are present in the mixture at a concentration of from 0.0002 mg/mL to 0.005 mg/mL. Most preferably, the manganese dioxide particles are present in the mixture at a concentration of from 0.0005 mg/mL to 0.003 mg/mL, such as 0.001 mg/mL.
In some examples, the mixture further comprises a capping agent. Preferably, the capping agent comprises least of: polyethylene at one glycol, ethylenediaminetetraacetic acid, polyvinyl pyrrolidone, polyvinyl alcohol, polyallylamine hydrochloride, dodecanthiol, or chitosan. Most preferably, the capping agent is a water-soluble polymer capping agent, such as polyvinyl pyrrolidone, polyethylene glycol, ethylenediaminetetraacetic acid, polyvinyl alcohol, or polyallylamine hydrochloride. The capping agent may be polyvinyl pyrrolidone.
In some examples, the manganese dioxide particles and/or the silver particles are dispersed in the water, such that the cleaning mixture is considered a dispersion.
The water in the cleaning mixture could for instance be deionized water, distilled water or tap water. The mixture could include additional components dissolved within the water, such as for instance sodium chloride in a saline solution. The saline solution may be phosphate buffered saline.
Preferably, the mixture further comprises hydrogen peroxide. In some examples, the mixture comprises up to 5 wt. % hydrogen peroxide, up to 3 wt. % hydrogen peroxide, up to 2 wt. % hydrogen peroxide, or up to 1.5 wt. % hydrogen peroxide. In some examples, the mixture comprises at least 0.1 wt. % hydrogen peroxide, at least 0.3 wt. % hydrogen peroxide or at least 0.5 wt. % hydrogen peroxide. Preferably, the mixture comprises 0.3 wt. % to 3 wt. % hydrogen peroxide. Most preferably, the mixture comprises 0.5 wt. % to 1.5 wt. % hydrogen peroxide, such as 1 wt. % hydrogen peroxide.
In some examples, the hydrogen peroxide is added to the mixture up to 15 minutes before the application of the mixture to the surface. Preferably, the hydrogen peroxide is added to the mixture up to 5 minutes before the application of the mixture to the surface with biofilm.
The first example cleaning mixture is prepared by a method comprising: preparing silver particles; preparing manganese dioxide particles; and mixing the silver particles and manganese dioxide particles in water.
Examples of the disclosure provide a method of producing manganese dioxide particles, comprising:
In some examples, the manganese (II) salt is manganese (II) acetate, manganese (II) sulphate, manganese (II) chloride, or manganese (II) nitrate. Preferably, the manganese (II) salt is manganese acetate.
In some examples, the acetate salt is manganese acetate or sodium acetate. Preferably, both the manganese (II) and acetate salts are manganese acetate.
Advantageously, it has been found that this method of forming manganese dioxide particles provides a well dispersed mixture, with a short reaction time. Furthermore, no heat source is required to perform the reaction. The method may be carried out at room temperature.
In some examples, the capping agent comprises at least one of: polyethylene glycol, ethylenediaminetetraacetic acid, polyvinyl pyrrolidone, polyvinyl alcohol, polyallylamine hydrochloride, dodecanthiol, or chitosan. Preferably, the capping agent is a water-soluble polymer capping agent, such as polyvinyl pyrrolidone, polyethylene glycol, ethylenediaminetetraacetic acid, polyvinyl alcohol, or polyallylamine hydrochloride. Most preferably, the capping agent is polyvinyl pyrrolidone.
In some examples, the oxidizing agent includes at least one of: potassium permanganate, ammonium persulfate or sodium chlorate. Preferably, the oxidizing agent is potassium permanganate.
Where the oxidizing agent is potassium permanganate and the manganese (II) and acetate salts are manganese acetate, the ratio of the moles of manganese acetate in the first solution to the moles of potassium permanganate added to the solution may be from 1:1 (mol Mn(CH3COO)2:mol KMnO4) to 2:1 (mol Mn(CH3COO)2:mol KMnO4). Preferably, the ratio of the moles of manganese acetate in the first solution to the moles of potassium permanganate added to the solution is 3:2 (mol Mn(CH3COO)2:mol KMnO4).
In some examples, the water is deionized water or distilled water.
In some examples, tannic acid is also mixed into the first solution prior to the addition of the oxidizing agent.
In some examples, the solution is stirred prior to and/or after the addition of the oxidizing agent. The oxidizing agent may be added in a dropwise fashion.
An example method for producing manganese dioxide particles includes adding aqueous polyvinyl pyrrolidone solution (1 mL, 1% w/v), aqueous manganese acetate solution (0.45 mL, 0.01 M), and aqueous tannic acid solution (1 mL, 0.001M) to deionized water (7 mL) under continuous stirring at 500 rpm in a 25 ml conical flask. To this solution, an aqueous solution of potassium permanganate (0.6 mL, 0.005 M) is added slowly in a dropwise fashion. After addition is complete, the mixture was left stirring for 15 minutes at room temperature prior to dilution in water (1:4 mixture:water), to provide a manganese dioxide particle stock mixture. The aqueous manganese acetate solution may be prepared by dissolving solid manganese acetate in deionized water. The aqueous potassium permanganate solution may be prepared by dissolving solid potassium permanganate in deionized water.
A comparative example method for producing manganese dioxide particles includes dissolving KMnO4 (3 g) in deionized water (800 mL), followed by the addition of ethyl acetate (120 mL). The reaction mixture is stirred continuously at 50-60° C. overnight. After reaction, the mixture is filtered and the manganese dioxide cake is washed with water and then ethanol. The sample is then dried in the oven overnight at 60° C. This comparative example method can produce manganese dioxide particles for use in the cleaning mixture, but is much slower than the example method described previously, and also requires a heat source.
An example silver particle synthesis includes adding aqueous silver nitrate solution (1 mL, 0.1 M) and aqueous polyvinylpyrrolidone solution (1 mL, 1% w/v) to deionized water (7 mL) under continuous stirring at 500 rpm in a 25 ml conical flask. To this solution, a reducing agent such as a freshly made-up aqueous solution of sodium borohydride (1 mL, 0.015 M) is added slowly in a dropwise fashion. After addition was complete, the mixture was left stirring for 15 minutes at room temperature, to form a silver particle stock mixture. Tollens' reagent [Ag(NH3)2]+ could be used in place of silver nitrate in other examples. An alternative reducing agent to sodium borohydride could be for instance sodium citrate, sodium ascorbate, lithium aluminum hydride, diisobutylaluminum hydride, or polyethylene glycol (PEG). The aqueous silver nitrate solution may be prepared by dissolving solid silver nitrate in deionized water.
To form the cleaning mixture for removing biofilm from surfaces, a silver particle mixture may be mixed with a manganese dioxide particle mixture. The silver particle mixture and the manganese dioxide particle mixture can be formed using any suitable method, such as those described above. As an example, 1 mL of the silver particle stock mixture described previously is mixed with 0.5 mL of the manganese dioxide particle stock mixture described previously, to provide a first stock cleaning mixture for removing biofilm from surfaces.
Preferably, the cleaning mixture for removing biofilm from surfaces is mixed with hydrogen peroxide prior to use. For instance, 1.5 mL of the first stock cleaning mixture could be diluted with 1 mL of deionized water, then 0.5 mL of aqueous 6 wt. % hydrogen peroxide solution could be added to the mixture.
A second example cleaning mixture for removing biofilm from surfaces comprises particles surface modified with silver, particles surface modified with manganese oxide, and water.
Preferably, the particles surface modified with silver and the particles surface modified with manganese oxide comprise particles surface modified with both silver and manganese oxide. In other words, at least some of the particles include both manganese oxide and silver on their outer surface. The particles surface modified with both manganese oxide and silver may be provided in the water at a concentration of 0.5 mg/ml of water to 1.5 mg/ml of water, such as 1 mg of surface modified particles per ml of water.
In some examples, the particles surface modified with silver and the particles surface modified with manganese oxide are zeolite particles surface modified with both manganese oxide and silver.
In some examples, the particles surface modified with both silver and manganese oxide have a hydrodynamic diameter of up to 100 μm, as measured using dynamic light scattering. The hydrodynamic diameter is the diameter of a hypothetical hard sphere that diffuses with the same speed as the particle being measured. The hydrodynamic diameters described herein are as measured using dynamic light scattering.
In some examples, the particles surface modified with both silver and manganese oxide have a hydrodynamic diameter of up to 70 μm, or up to 50 μm. The particles surface modified with both silver and manganese oxide may have a hydrodynamic diameter of 20 μm to 100 μm, such as 50 μm to 70 μm.
In some examples, the loading of manganese on surface of the particles is between 0.5 wt. % and 10 wt. %. This wt. % loading is the total weight of manganese (i.e., manganese/manganese ions, not manganese oxide) added to the particles, calculated as a percentage of the total weight of the particles before surface modification. Preferably, the loading of manganese on the particles is between 1 wt. % and 5 wt. %. Most preferably, the loading of manganese on the particles is between 2 wt. % and 4 wt. %, such as 3 wt. %.
In some examples, the loading of silver on the surface of the particles is between 20 wt. % and 80 wt. %. This wt. % loading is the total weight of silver (i.e., silver/silver ions, not silver oxide) added to the particles, calculated as a percentage of the total weight of the particles before surface modification. Preferably, the loading of silver on the particles is between 30 wt. % and 60 wt. %. Most preferably, the loading of silver on the particles is between 35 wt. % and 50 wt. %, such as 43 wt. %.
In some examples, the particles surface modified with silver and the particles surface modified with manganese oxide are dispersed in the water, such that the cleaning mixture is considered a dispersion.
The water in the cleaning mixture could for instance be deionized water, distilled water or tap water. The mixture could include additional components dissolved within the water, such as for instance sodium chloride in a saline solution. The saline solution may be phosphate buffered saline.
Preferably, the mixture further comprises hydrogen peroxide. In some examples, the mixture comprises up to 5 wt. % hydrogen peroxide, up to 3 wt. % hydrogen peroxide, up to 2 wt. % hydrogen peroxide, or up to 1.5 wt. % hydrogen peroxide. In some examples, the mixture comprises at least 0.1 wt. % hydrogen peroxide, at least 0.3 wt. % hydrogen peroxide or at least 0.5 wt. % hydrogen peroxide. Preferably, the mixture comprises 0.3 wt. % to 3 wt. % hydrogen peroxide. Most preferably, the mixture comprises 0.5 wt. % to 1.5 wt. % hydrogen peroxide, such as 1 wt. % hydrogen peroxide.
In some examples, the hydrogen peroxide is added to the mixture up to 15 minutes before the application of the mixture to the surface. Preferably, the hydrogen peroxide is added to the mixture up to 5 minutes before the application of the mixture to the surface with biofilm.
The second example cleaning mixture is prepared by a method comprising: surface modifying particles with silver; surface modifying particles with manganese oxide; and mixing the surface modified particles in water. Preferably, the same particles are surface modified with both silver and manganese oxide. Most preferably, the particles are zeolite particles, which are surface modified with both silver and manganese dioxide.
An example synthesis of the manganese oxide surface modified particles and the silver surface modified zeolite particles includes dispersing 0.5 g of zeolite Y (Alfa Aesar Zeolite Y, sodium, product number 45862) in 100 mL of deionized water under stirring (500 rpm) for few minutes. To that mixture, 1 mL of 28 wt. % concentrated ammonium hydroxide, 0.5 mL of tetraethyl orthosilicate (TEOS) and 1 mL of (3-aminopropyl) triethoxysilane (APTES) were slowly added. This mixture was heated under stirring in a water bath at 50° C. until dried, providing a white solid of amino-functionalized zeolite. After drying, a 10 ml of a 0.05 M aqueous solution of potassium permanganate, (KMnO4) and 80 mL of a 0.1 M aqueous solution of silver nitrate, (AgNO3) were mixed with the amino-functionalized zeolite. The mixture was then placed in an ultrasonic bath for 30 minutes at 45 kHz. The mixture is then dried in a water bath at 50° C. to provide the manganese oxide and silver surface modified zeolite particles.
As a first step, to form the sample biopsy tubes harboring the biofilm, the biopsy channel was first cut into small pieces, which are then submerged into Müller-Hilton broth. A single colony of Pseudomonas aeruginosa was added to the broth. At a second step, the pieces were kept in the broth at 37° C. for 24 hours to allow the bacteria to grow. Also in the second step, the tube pieces were then washed 5 times with a copious amount of water to remove dangling bacteria, and to leave only fixed biofilm remaining on the surface of the tubes. The second step is then repeated three times to provide a mature biofilm.
To provide the data of
From left to right along the chart of
It has therefore been found that silver containing particles and manganese oxide containing particles work synergistically to very quickly remove a wide range of pathogens from the surface, such as gram-positive bacteria, gram-negative bacteria, and fungi. The cleaning mixtures described herein significantly reduce the amount of all of the tested pathogens very quickly, which is even further enhanced with the addition of hydrogen peroxide.
To obtain hydrodynamic diameter measurements of the particles described herein, dynamic light scattering may be used.
In one example, a Nanobrook 90PLUS instrument is used to obtain the dynamic light scattering measurements. In this example, to obtain the dynamic light scattering measurements of the silver particles in the silver particle stock mixture described above, the silver particle stock mixture is diluted using deionized water in the ratio 1:5 (silver particle stock mixture:deionized water). Around 2 ml of the diluted solution is then transferred by pipette into a polystyrene cuvette. The cuvette is then placed into the sample holder of the Nanobrook 90PLUS instrument and the measurement is conducted. The measurement time is 1 minute. The instrument then determines the hydrodynamic diameter. The hydrodynamic diameter is sometimes also known as the “effective diameter”. An average hydrodynamic diameter can then be determined by repeating the measurement 3 times, and calculating the mean value of the 3 repeat measurements. The same procedure can be used to measure the hydrodynamic diameter of the manganese dioxide particles, using the manganese dioxide particle stock mixture rather than the silver particle stock mixture.
Examples of the disclosure provide a method of removing biofilm from a surface, the method comprising: applying the cleaning mixtures described herein to the surface.
In the example system shown in
The drain 130 is located at the bottom of the bath 150. In this example, the drain is connected to a drain valve 132, which is configured to control the draining of fluid from the bath 150. The drain 130 may also be connected to a drain pump 134, which is configured to accelerate the draining of fluid from the bath 150.
In this example, the inlet 110, which is arranged to direct fluid into the internal channel of the endoscope 200, is fluidly connected to the bath 150, via an outlet 112 from the bath 112. In this example the outlet 112 from the bath 150 is located at the bottom end of the bath 150. A pump 170, such as a peristaltic pump, may be provided between the outlet 112 from the bath 150 and the inlet 110 into the internal channel of the endoscope 200, to control the flow of liquid from the bath 150 into the endoscope 200. The endoscope 200 includes an opening, for instance at the end of the biopsy channel, to enable the liquid to flow back into the bath 150 once it has passed through the endoscope 200.
In some examples, the bath 150 further includes a sonicator 180, such as an ultrasonic sound emitter, which is configured to agitate the liquid and endoscope 200 within the bath 150. Furthermore, the bath 150 might include a level sensor 190, as shown in
As described previously,
In some examples, an enzymatic solution is applied to the surface prior to the application of the cleaning mixture, as shown in block 510 of
In this example, the enzymatic solution is then supplied to the inlet 110 and into the internal channel of the endoscope 200. In the example of
After a period of time, the enzymatic solution is drained from the bath 150 via the drain 130, until the bath is empty, as illustrated in
In some examples, the surface with biofilm is then rinsed, as shown in block 520 of
To rinse the internal channel of the endoscope 200, the water is supplied to the inlet 110, which is arranged to direct the fluid into the internal channel of the endoscope 200. In the example of
After a period of time, the water is drained from the bath 150 via the drain 130, until the bath is empty, as illustrated in
The cleaning mixture is applied to the surface, as shown in block 530 of
In some examples, the cleaning mixture is applied to the surface for up to 15 minutes. Preferably, the cleaning mixture is applied to the surface for up to 10 minutes. Most preferably, the cleaning mixture is applied to the surface for 2.5 minutes to 7.5 minutes, such as 5 minutes.
To remove biofilm from the internal channel of the endoscope 200, the cleaning mixture is supplied to the inlet 110 and into the internal channel of the endoscope 200. In the example of
After a period of time, the cleaning mixture is drained from the bath 150 via the drain 130, until the bath is empty, as illustrated in
In some examples, the surface is then rinsed, as shown in block 540 of
There is thus described a cleaning mixture, a system and a method for removing biofilm from a surface with a number of advantages. The elements of the cleaning mixture work synergistically to provide a very powerful agent for removing biofilm. The biofilm can be removed very quickly, which provides a substantial advantage in hospitals where slow turnaround times can significantly increase costs. A wide spectrum of pathogens within the biofilm are also eradicated, including gram-positive bacteria, gram-negative bacteria, and fungi, which significantly reduces the infection risk from the surface. Furthermore, the mixture, system, and method do not require the use of mineral acids or other powerful acids, so biofilms can be removed from metallic surfaces without damaging or corroding the surface and surrounding elements.
In this description, reference has been made to various examples. The description of features or functions in relation to an example indicates that those features or functions are present in that example. The use of the term ‘example’ or ‘for example’ or ‘can’ or ‘may’ in the text denotes, whether explicitly stated or not, that such features or functions are present in at least the described example, whether described as an example or not, and that they can be, but are not necessarily, present in some of or all other examples. Thus ‘example’, ‘for example’, ‘can’ or ‘may’ refers to a particular instance in a class of examples. A property of the instance can be a property of only that instance or a property of the class or a property of a sub-class of the class that includes some but not all of the instances in the class. It is therefore implicitly disclosed that a feature described with reference to one example but not with reference to another example, can where possible be used in that other example as part of a working combination but does not necessarily have to be used in that other example.
Although examples have been described in the preceding paragraphs with reference to various examples, it should be appreciated that modifications to the examples given can be made without departing from the scope of the claims. For example, the cleaning mixture can be applied to a variety of surfaces, including flat, rounded, internal and/or external surfaces of a variety of objects. The mixture, method and system could be used in the food, medical, or dental industries. The cleaning mixture can be applied to the surface using a variety of methods, including for example brushing, spraying or flushing. The particles can be made using a variety of methods. The particles can be effective at a variety of sizes. Different particles for surface modification with silver or manganese oxide could be used, including other oxides such as silica particles, alumina particles or titania particles. The cleaning mixture, water, hydrogen peroxide solution and/or enzymatic solution could be added to the bath by other means. For instance, the bath might include only one inlet through which all the liquids pass into the bath.
It is to be appreciated that the silver particles could be made purely of silver, or contain some impurities such as silver oxide. Furthermore, it is to be appreciated that the manganese dioxide particles could be made purely of manganese dioxide, or contain some impurities such as manganese (III) oxide.
The manganese oxide provided on the example surface modified particles could include manganese in different oxidation states, such as oxidation states of +2 or +4. The oxidation state could depend on, for instance, the substrate and/or the particular surface site at which the manganese ion is located. Similarly, the silver provided on the surface modified particles could include silver in different oxidation states, such as oxidation states of 0 or +1.
Features described in the preceding description may be used in combinations other than the combinations explicitly described above. For instance, the cleaning mixture may include a combination of silver particles and manganese oxide surface modified particles, or a combination of manganese dioxide particles and silver surface modified particles. Where multiple ranges in the format “from X to Y” are described in relation to a certain feature, it is understood that all ranges combining the different endpoints are also contemplated.
Although functions have been described with reference to certain features, those functions may be performable by other features whether described or not.
Although features have been described with reference to certain examples, those features may also be present in other examples whether described or not.
The term ‘comprise’ is used in this document with an inclusive not an exclusive meaning. That is any reference to X comprising Y indicates that X may comprise only one Y or may comprise more than one Y. If it is intended to use ‘comprise’ with an exclusive meaning then it will be made clear in the context by referring to “comprising only one” or by using “consisting”.
The term ‘a’ or ‘the’ is used in this document with an inclusive not an exclusive meaning. That is any reference to X comprising a/the Y indicates that X may comprise only one Y or may comprise more than one Y unless the context clearly indicates the contrary. If it is intended to use ‘a’ or ‘the’ with an exclusive meaning then it will be made clear in the context. In some circumstances the use of ‘at least one’ or ‘one or more’ may be used to emphasize an inclusive meaning but the absence of these terms should not be taken to infer any exclusive meaning.
The presence of a feature (or combination of features) in a claim is a reference to that feature or (combination of features) itself and also to features that achieve substantially the same technical effect (equivalent features). The equivalent features include, for example, features that are variants and achieve substantially the same result in substantially the same way. The equivalent features include, for example, features that perform substantially the same function, in substantially the same way to achieve substantially the same result.
In this description, reference has been made to various examples using adjectives or adjectival phrases to describe characteristics of the examples. Such a description of a characteristic in relation to an example indicates that the characteristic is present in some examples exactly as described and is present in other examples substantially as described.
Whilst endeavoring in the foregoing specification to draw attention to those features believed to be of importance it should be understood that the applicant may seek protection via the claims in respect of any patentable feature or combination of features hereinbefore referred to and/or shown in the drawings whether or not emphasis has been placed thereon.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2114645.1 | Oct 2021 | GB | national |
This application is a national stage filing under 35 U.S.C. § 371 of international application number PCT/GB2022/052505, filed on Oct. 4, 2022, which claims priority to British patent application No. 2114645.1, filed Oct. 13, 2021. The contents of these applications are incorporated herein by reference in their entirety.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/GB22/52505 | 10/4/2022 | WO |
