Claims
- 1. A method of generating a living biological matrix in vitro, the method comprising:
(a) obtaining a cell sample; (b) disrupting the cell sample to create a mixture containing cells and cellular debris; (c) culturing the mixture, retaining the cellular debris, in culture medium for a time and under conditions sufficient to form a living biological matrix in vitro; and (d) removing the biological matrix from the culturing medium.
- 2. The method of claim 1, wherein the cell sample of step (a) is obtained from a subject who will be a recipient of the biological matrix.
- 3. The method of claim 1, wherein the cell sample of step (a) is obtained from a human.
- 4. The method of claim 1, wherein the cell sample comprises a bodily fluid.
- 5. The method of claim 4, wherein the bodily fluid is blood.
- 6. The method of claim 4, wherein the bodily fluid is cerebrospinal fluid.
- 7. The method of claim 1, wherein the cell sample comprises a portion of an organ.
- 8. The method of claim 1, wherein the cell sample comprises auricular cartilage.
- 9. The method of claim 8, wherein before disrupting the cell sample, the perichondrium is removed from the cartilage.
- 10. The method of claim 1, further comprising adding to the mixture a component that adds shape, structure, or support to the matrix.
- 11. The method of claim 10, wherein the component is a hydrogel or an adhesive.
- 12. The method of claim 1, further comprising adding to the matrix an antibiotic.
- 13. A method of augmenting a tissue defect in a subject, the method comprising:
(a) preparing a living biological matrix using the method of claim 1; and (b) administering the living biological matrix to the subject in the region of the tissue defect, wherein the matrix develops a characteristic of the endogenous tissue and thereby augments the tissue defect.
- 14. The method of claim 13, wherein the tissue defect is in a muscle.
- 15. The method of claim 14, wherein the muscle is the heart.
- 16. The method of claim 13, wherein the tissue defect is in a portion of a lung, pancreas, spinal cord, joint, head, neck, skin, kidney, or liver of the subject.
- 17. The method of claim 13, wherein the subject is a human.
- 18. A living biological matrix comprising a spore-like cell, cell fragments, lipids, and polysaccharides.
- 19. The matrix of claim 18, further comprising a component that adds shape, structure, or support to the matrix.
- 20. The matrix of claim 18, further comprising a hydrogel or adhesive.
- 21. The matrix of claim 18, further comprising an antibiotic.
- 22. The matrix of claim 18, further comprising a cellular component selected from the group consisting of a fibronectin, laminin, collagen, glycoprotein, thrombospondin, elastin, fibrillin, mucopolysaccharide, glycolipid, heparin sulfate, chondroitin sulfate, keratin sulfate, glycosaminoglycan, and hyaluronic acid.
- 23. A method of augmenting a tissue defect in a subject, the method comprising:
(a) obtaining a living biological matrix of claim 18; and (b) administering the living biological matrix to the subject in the region of the tissue defect, wherein the matrix develops a characteristic of the endogenous tissue and thereby augments the tissue defect.
- 24. A living biological matrix produced by a process comprising:
(a) obtaining a cell sample; (b) disrupting the cell sample to create a mixture containing cells and cellular debris; (c) culturing the mixture, retaining the cellular debris, in culture medium for a time and under conditions sufficient to form a biological matrix in vitro; and (d) removing the biological matrix from the culture medium.
- 25. The matrix of claim 24, wherein the cell sample of step (a) is obtained from a subject who will be a recipient of the biological matrix.
- 26. The matrix of claim 24, wherein the cell sample is obtained from a human.
- 27. The matrix of claim 24, wherein the cell sample comprises a bodily fluid.
- 28. The matrix of claim 27, wherein the bodily fluid is blood.
- 29. The matrix of claim 27, wherein the bodily fluid is cerebrospinal fluid.
- 30. The matrix of claim 24, wherein the cell sample comprises a part of an organ.
- 31. The matrix of claim 24, wherein the cell sample comprises auricular cartilage.
- 32. The matrix of claim 31, wherein, before disrupting the cell sample, the perichondrium is removed from the cartilage.
- 33. The matrix of claim 24, wherein the process further comprising adding to the mixture a component that adds shape, structure, or support to the matrix.
RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Application No. 60/419,682, filed on Oct. 17, 2002, the subject matter of which is incorporated herein by reference in its entirety.
Provisional Applications (1)
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Number |
Date |
Country |
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60419682 |
Oct 2002 |
US |