Biologically active agents and drugs

FIELD OF INVENTION

[0001] The present invention relates to medicine and bioactivator useful as cosmetics, freshness preservative agent, growth promoting agent of animals and plants, and the like.

PROBLEM THAT THE PRESENT INVENTION INTENDS TO SOLVE

[0002] For instance, iron salt such as ferric ferrous iron salt is bioactivated and known to be useful as medicine, cosmetics, freshness preservative agent, growth promoting agent of animals and plants and the like.

[0003] Nevertheless, there is a problem that bioactive ability of said iron salt is unstable so that effect of said iron salt deteriorates during long preservation.

DISCLOSURE OF THE INVENTION

[0004] The gist of the present invention is that magnesium salt is added to said iron salt as stabilizer as means to solve the above described existing problem.

[0005] It is desirable that said iron salt and said magnesium salt are mixed together at a molar ratio in the range between 1:1 to 1:106 and further, it is desirable that said bioactivator is prepared as aqueous solution and said iron salt in said aqueous solution is contained at a concentration in the range between 10−12 to 5 moles and further said iron salt is desirably ferric-ferrous iron salt. Further, the present invention provides treatment medicine of diabetes, hypertension, cancer, hepatitis, rheumatism, atopic dermatitis and the like said treatment medicine being said bioactivator.

DESCRIPTION OF THE INVENTION

[0006] The present invention is explained precisely hereafter.

[0007] Iron salt(s) of the present invention is(are) ferric-ferrous iron salt and/or ferrous iron salt and/or ferric iron salt.

[0008] [Ferrous Iron Salt, Ferric Iron Salt]

[0009] Ferrous iron salt and/or ferric iron salt, used as bioactivator in this invention, include inorganic acid salts such as hydrochloride, sulfate, nitrate, phosphate and the like, organic acid salts such as acetate, formate, oxalate, citrate, lactate, butyrate, succinate, propionate and the like, mixtures thereof. Two or more kinds of ferrous iron salt and/or ferric iron salt may be used together.

[0010] [Ferric-Ferrous Iron Salt]

[0011] Ferric-ferrous iron salt of the present invention is iron salt having properties between ferrous iron salt and ferric iron salt and said iron salt is such as inorganic acid salts (e.g. hydrochloride, sulfate, phosphate, nitrate and the like), organic acid salts (e.g. formate, acetate, oxalate citrate, lactate, butyrate, succinate, propionate and the like). Said ferric-ferrous iron salt is prepared by putting ferric iron salt in a large quantity of strong alkaline aqueous solution such as sodium hydroxide, potassium hydroxide, lithium hydroxide, calcium hydroxide and the like to cause valence conversion from ferric iron to ferrous iron or putting ferrous iron salt in a large quantity of strong acid aqueous solution such as hydrochloride acid, sulfuric acid and the like to cause valance convertion from ferrous iron to ferric iron and said ferric-ferrous iron salt is obtained as transition form during said valence conversion. Concrete illustrations of methods of production of said ferric-ferrous iron salt are shown hereafter.

[0012] Commonly, two methods described below are applied to prepare said ferric-ferrous iron salt.

[0013] 1. Method 1 (Preparation from Ferric Iron Salt)

[0014] Ferric chloride (FeCl3.6H2O), 1.0 mg was dissolved in 100 ml of 0.5 N sodium hydroxide aqueous solution and stirred, then the solution was allowed to stand for overnight. After filtering out some insoluble products in the solution, the solution was neutralized with hydrochloric acid then concentrated in a reduced pressure desiccator to get a dried and crystallized product. Thus the crystallized product with sodium chloride, that is, chloride of ferric-ferrous iron (hereinafter sometimes referred to as iron chloride (II, III)), was prepared.

[0015] In case of extracting iron chloride (II, III) from the crystallized product with sodium chloride, the product was dissolved in 50 ml of 80% by weight isopropyl alcohol aqueous solution to elute iron chloride (II, III). After separating the solution containing eluted iron chloride (II, III), the solution was concentrated at reduced pressure in order to remove the solvent and dry. Then the procedure consisting of elution, concentration and dry was repeated a few times. Thus iron chloride (II, III), 0.25 mg was extracted from the crystallized product with sodium chloride.

[0016] 2. Method 2 (Preparation From Ferrous Iron Salt)

[0017] Ferrous sulfate (FeSO4.7H2O), 1.0 mg was dissolved in 100 ml of 0.5 N HCl aqueous solution and stirred, then the solution was allowed to stand for overnight. After filtering out some insoluble products in the solution, the solution was concentrated in a reduced pressure desiccator to get a dried product. The dried product in powder was dissolved in 10 ml of 80% by weight isopropyl alcohol aqueous solution to elute iron chloride (II, III). After separating the solution containing eluted iron chloride (II, III), the solution was concentrated at reduced pressure in order to remove the solvent and dry. Then the procedure consisting of elution, concentration and dry was repeated a few times. Thus iron chloride (II, III), 0.6 mg was extracted from the crystallized product with sodium chloride.

[0018] Efficacy of bioactivation of said iron salts are as follows.

[0019] Ferric-ferrous iron salt>ferrous iron salt>ferric iron salt

[0020] [Magnesium Salt]

[0021] Magnesium salts, used in this invention, include inorganic acid salts such as magnesium chloride, magnesium sulfate, magnesium phosphate, magnesium nitrate, organic acid salts such as magnesium acetate, magnesium butyrate, magnesium formate, magnesium oxalate, magnesium citrate, magnesium propionate, and mixtures thereof. Two or more kinds of magnesium salts may be used together.

[0022] [Preparation]

[0023] Commonly said iron salt and said magnesium salt are respectively prepared as aqueous solution. In said aqueous solution said iron salt and said magnesium salt are mixed in a molar ration in the range between 1:1 to 1:106 and commonly said iron salt is contained in said aqueous solution in a concentration in the range between 10−12 to 5 moles.

[0024] Further vitamin, hormone, fat and oil, perfumery spices, sweetening, and the like may be added in said aqueous solution.

[0025] Bioactivator of the present invention is mainly administered orally or by mixing in food as it is further said bioactivator can be administered by injection, instillation or percutaneously.

[0026] Said bioactivator of the present invention is especially useful for treatment or prevention of cancer, diabetes, hepatitis, nephritis, renal failure gastric ulcer, duodenal ulcer, hypertension, collagen disease, allergic diseases such as atopic dermatitis, pollinosis and the like, menorrhagia, obstipation, and the like and further useful as antimicrobial agent.

[0027] Further, said bioactivator of the present invention is useful as cosmetics since said bioactivator has beautificative action of skin besides preventive or treatment action of dermatitis and further said bioactivator has promotive action of growth of animals and plants and improving sense of taste.

[0028] [Action]

[0029] In the present invention, since iron salt useful as bioactivator is stabilized by magnesium salt, medicine, cosmetics, freshness preservative agent, growth promoting agent of animals and plants, and the like which can be preserved for longtime can be provided.

PREFERRED EMBODIMENT TO PUTTING THE INVENTION INTO PRACTICE

EXAMPLE 1

[0030] Ferrous sulfate (FeSO4.6H2O), 1 g was dissolved in 5 ml of 12 N HCl aqueous solution and stirred. Then the solution was filtered by filter paper (No. 5C) to remove some insoluble products. A portion of the filtered solution for sampling was concentrated in a reduced pressure desiccator to get a dried product. The dried product in powder was dissolved in 80% by weight isopropyl alcohol aqueous solution. Then the solution containing eluted component was concentrated at reduced pressure in order to remove the solvent and dry. In addition, the procedure consisting of elution, concentration and dry was repeated a few times. Thus crystallized product was prepared. 5% by weight aqueous solution of the crystallized product, 0.01 ml was spotted on a point from 3 cm of the bottom of paper chromatography (PC) filter paper (2 cm×40 cm), then was developed by n-butyl alcohol:acetic acid:H2O (5:1:4, v/v/v) as developing solvent for 15 hours. After developing the filter paper was dried out then colored by spray of 1% by weight potassium ferricyanide aqueous solution as coloring reagent. As a result, it was confirmed that the developed point of the crystallized product was one spot (Rf=0.07).

[0031] In addition, a mixture of FeCl2 and FeCl3 was spotted on a paper chromatography (PC) filter paper as the same way. As a result, it was confirmed that there were two developed points (FeCl2, Rf=0.095, FeCl3, Rf=0.36) on the filter paper. These paper chromatography (PC) tests mentioned above accounted for the crystallized product as homogeneous product not mixtures.

[0032] Further, a sample solution, 100 ml was prepared by means of dissolving the crystallized product in distilled water. The sample solution (2.5 ml), 0.1% by weight orthophenanthroline aqueous solution (2.5 ml), and sodium acetate-acetic acid buffer solution, pH=4.5, (25 ml) were put into a mess-flask then distilled water was put into the mess-flask until its marked line. After being allowed to stand for 30 minutes at room temperature, an absorbance (510 nm) of the solution was measured. Ferrous iron in the sample solution was 0.019 g/100 ml calculated from standard curve, obtained by FeCl2 solution in the same way.

[0033] Moreover, in the case of putting sample solution into the mess-flask, then hydroxyl mine hydrochloride aqueous solution, 1.0 ml was added to the mess-flask beforehand in order to reduce ferric iron in the sample solution to ferrous iron. As a result, ferrous iron, 0.038 g/100 ml was gotten. It was confirmed that the crystallized product consisted of ferrous iron and ferric iron equivalently because of calculation of ferric iron, 0.019 g/100 ml (=0.038 g/100 ml−0.019 g/100 ml). From consideration of the above-mentioned test, it was concluded that the crystallized product would be Fe2Cl5.xH2O.

EXAMPLE 2

Preparation Of Ferrous-Ferric Iron Salt

[0034] Ferric chloride (1.0 mg) was dissolved in 5 ml of 10 N sodium hydroxide aqueous solution and stirred. After stirring, the solution was neutralized with 10 N hydrochloric acid, then was filtered by a filter paper (No. 5C) to remove some insoluble products. A portion of the filtered solution for sampling was concentrated in a reduced pressure desiccator to get a dried product. The dried product in powder was dissolved in 80% by weight isopropyl alcohol aqueous solution. Then the solution containing eluted component was concentrated at reduced pressure in order to remove the solvent and dry. In addition, the procedure consisting of elution, concentration and dry was repeated a few times. Thus the crystallized product was prepared. The crystallized product in this example was tested by the same way as Example 1 mentioned above. Thus, it was concluded that the crystallized product would be Fe2Cl5.xH2O.

EXAMPLE 3

Preparation Of Bioactivator 1

[0035] Ferrous chloride (FeCl2) anhydride and magnesium chloride (MgCl2) anhydride were dissolved in water to prepare a bioactivator 1 in which 1×10−1 mol/l (FeCl2) and 2×10−12 mol/l (MgCl2) were contained.

EXAMPLE 4

Preparation Of Bioactivator 2

[0036] Ferrous sulfate (FeSO4) anhydride and magnesium sulfate (MgSO4) anhydride were dissolved in water to prepare a bioactivator 2 in which 1×10−6 mol/l (FeSO4) and 3×10−6 mol/l (MgSO4) were contained.

EXAMPLE 5

[0037] Preparation Of Bioactivator 3

[0038] Ferric-ferrous iron prepared in Example 1 and magnesium chloride (MgCl2) anhydride were dissolved in water to prepare a bioactivator 3 in which 1×10−10 mol/l (Fe2Cl5) and 2×10−8 mol/l (MgCl2) were contained.

EXAMPLE 6

Preparation Of Bioactivator 4

[0039] Ferric-ferrous iron prepared in Example 2 and magnesium sulfated (MgSO4) anhydride were dissolved in water to prepare a bioactivator 4 in which 1×10−12 mol/l (Fe2Cl5) and 1×10−2 mol/l (MgSO4) were contained.

EXAMPLE 7

Freshness Maintenance Test

[0040] Slices of a flatfish were dipped in the bioactivator 1 having been stored for 6 months after preparation, and then water was removed from said slices by a filter paper. Said slices wrapped in polyvinylidene chloride film were then kept at 5° C. K value (i.e. a value for determination of fish freshness) after said slices had been kept for 10 days was about 40 so that said slices of the flatfish were insufficiently eatable condition.

[0041] [Comparison 1]

[0042] Slices of the flatfish were dipped in an aqueous solution in which 1×10−12 mol/l (FeCl2) was contained and said aqueous solution had been stored for 6 months (without MgCl2) in Comparison 1. After then, water was removed from said slices by the filter paper and said slices were then kept at 5° C. K value after said slices had been kept for 10 days was about 60 so that said slices at the flat fish was in barely eatable condition.

[0043] [Comparison 2]

[0044] The same maintenance test was carried out by using slices of the flatfish which was dipped in water as the test of Comparison 2. K value after said slices had been stored for 10 days was about 70 and said slices of the flatfish was in uneatable condition.

[0045] As the results of Example 7 and Comparison 1 and 2, it was recognized that FeCl2 keeps sufficient effect to maintain freshness by adding MgCl2 even after six months preservation.

EXAMPLE 8

[0046] Using the bioactivator 2 which was prepared one year ago, pumpkins, potatoes and onions are cultivated. Conditions of said harvested vegetables were described below.

[0047] Pumpkins

[0048] Appearance: Having a glossy appearance and pulp is thick and has bright orange color and contains carotene as much as two times of ordinary pumpkins.

[0049] Taste: Being not soggy and tasting very good and sweet. Said pumpkin has a very high sugar content of 10.7 degree (generally 7 degree).

[0050] Potatoes

[0051] Appearance: Skin was white and having a little number of buds on the surface.

[0052] Starch: 18.6% (generally 16%).

[0053] Vitamin C: 32 mg/100 g (generally 23 mg/100 g).

[0054] Taste: Perfect degree of softness and being easily crushed in the mouth and having special smell and body of potato. Further, said potatoes are suitable for salad use since said potato has little harshness.

[0055] Onions

[0056] Appearance: Having a glossy appearance and uniform size. Skin can be easily peeled and pulp is tight and firm. Being storable for a long time. It was recognized by the electron microscope that said onion was a healthy crop having tissue in which small cells are packed closely.

[0057] Taste: Being easily cut by a kitchen knife and having good taste far eating raw. Since sugar content degree of said onion is very high, 10 degree, and has pleasant feeling on biting so that said onion is suitable for salad and does not crumble by frizzling.

EXAMPLE 9

Medical Efficacy

[0058] In a case where said bioactivator 3 prepared in Example 5 is used as medicine, generally the following drinking method is applied.

[0059] (1) The following quantity of said bioactivator 3 is added in a cup of water (about 150 ml) and mixed well and said diluted bioactivator is drunk three times in a day, at getting up, before lunch, and before going to bed.

[0060] (2) Drinking quantity

[0061] First one week: 3 drops×3 times (9 drops in a day)

[0062] Second week: 5 drops×3 times (15 drops in a day)

[0063] Third week: 10 drops×3 times (30 drops in a day)

[0064] Forth week: 20 drops×3 times (60 drops in a day)

[0065] (3) Final drinking quantity

[0066] a. Cancer: 30 drops×3 times (90 drops in a day)

[0067] b. Diabetes, Hepatitis, Gastric ulcer, Heart disease, Asthma, Hypertension, etc.: 20 drops×3 times (60 drops in a day)

[0068] c. Renal failure Rheumatism, Atopic dermatitis, Pollinosis, etc.: 10 drops×3 times (30 drops in a day)

[0069] d. Menorrhagia, Obstipasion, Sick from drinking, other slight diseases: 10 drops×one time (10 drops in a day)

[0070] e. Maintenance of health: 3 drops×3 drops (9 drops in a day)

[0071] The results in a case where said bioactivator 3 was administered to patients having various diseases according to above described drinking method are shown in Tables 1 to 14.

1TABLE 1inspection datathe name ofpatientsthe bloodHbAlcfatblood pressurecasesa diseasesexagesugar level *1*2*3*4observatin1diabetesmale693189.2331Numerical value was improved28110.2235as shown in Table afterdrinking for one week.2diabetesfemale65360172Numerical value was improved273126as shown in Table afterdrinking for three months,and diabetic polyneuropathywas also improved at the same time.3diabetesmale58328Numerical value was improved119as shown in Table after drinkingfor three months although dietetictreatment had been unstable.4diabetesfemale583169At the start to drink, taking1416.220 units of insulin and 2tablets of blood sugar descendingagent but stopped taking them andreduced blood pressure descendingagent from 12 tablets to 4 tablets.5diabetesfemale60287143/76Inspection data was much87123/74improved and hypertension wasalso improved without dosageof blood pressure descendingagent after drinking for three months.6diabetesmale55131142/88All subjective symptoms were80126/88much improved after drinkingfor three months.7diabetesfemale39336She was a patient who was190diagnosed by the other hospitalthat dosage of insulin wasnecessary, but numerical valuewas improved as shown in Tableafter drinking and taking only onetablet of blood sugar descendingagent for three months in this hospital.Top: befor, Bottom: after *1 the blood sugar level: normal values 70-110 ml/dl *2 HbAlc: normal values 4.0-6.0% *3 fat: normal values 50-140 mg/dl *4 blood pressure: normal values 139-101/89-61 mmHg

[0072]

2

TABLE 2

inspection data

the blood

blood

the name of
patients
sugar
HbAlc
fat
pressure

cases
a disease
sex
age
level *1
*2
*3
*4
observation

8
diabetes
male
55
458
12.4

After leaving a general hospital,

98
5.4

he was treated in this hospital.

After drinking for one month,

stopped dosage of insulin. Numerical

value was improved as shown in

Table after drinking for three months.

9
diabetes
female
40

12

Injection of insulin by himself since

7.6

he was 18 years old. He had had complete

dietetic treatment and kinesitherapy and

control of weigh so that there was no way

excepting increasing dosage of insulin

but numerical value was improved as

shown in Table and dosage of insulin

could be reduced.

10
diabetes
male
40
823
13.8

Numerical value was improved as shown

89
6.2

in Table after drinking for three months.

11
diabetes
male
65
247
8.7

Stopped completely to take dosage

93
6.8

of insulin after drinking for six months.

12
diabetes
female
72
440
12.2

He was a patient to whom a big quantity

151
5.9

(32 units) of insulin had been given but

dosage of insulin could be reduced to

22 units after drinking π water for

three months and inspection data was

improved. Further improvement is

expected here after.

13
diabetes
female
42
360
10.4

He had insulin treatment for more

74
7.6

than 20 years but improved on

numerical value after drinking

for three months even dosage of

insulin was reduced.

14
diabetes
male
59
316
8.5

First taking 20 units of insulin

140
5.9

and 14 tablets of oral medicine.

After drinking for three months,

dosage of insulin could be reduced

to 0 and oral medicine could be

reduced to 2 tablets.

Top: befor,

Bottom: after

*1 the blood sugar level: normal values 70-110 ml/dl

*2 HbAlc: normal values 4.0-6.0%

*3 fat: normal values 50-140 mg/dl

*4 blood pressure: normal values 139-101/89-61 mmHg

[0073]

3

TABLE 3

inspection data

the name of
patients
the blood
HbAlc
fat
blood pressure
fructosamine

cases
a disease
sex
age
sugar level *1
*2
*3
*4
*5
observation

15
diabetes
male
55
520
12.4

He had insulin treatment in the other

85
55

hospital but there was no improvement

before visiting this hospital. Completely

recovered after drinking for less than

one month.

16
diabetes
male
53
311
12.3

Improved by drinking for three months.

96
7.4

He had high blood pressure and tookd

the blood pressure descending agent

but at present the blood pressure

became normal and not necessary to

take medicine.

17
diabetes
male
48
668

First he had taken insulin. After

87

drinking for six months, he

recovered to the condition that

insulin taking was not necessary.

18
diabetes
female
64
331
12.3
264

5.9
After drinking for three months,

96
7.4
66

2.68
diabetes was improved and fat

value was also improved.

19
diabetes
female
39
336

She was in condition that medication of

190

insulin was necessary but numerical value

was improved without medication of

insulin, as shown in Table after drinking

for three months.

20
diabetes
female
60
287

143/76
She had complication of hypertension but

87

123/74
much improved by drinking for one month

without medication.

21
diabetes
male
58
326
10.4

Numerical value was improved

168
7.1

as shown in Table by drinking

for six weeks.

Top: befor,

Bottom: after

*1 the blood sugar level: normal values 70-110 ml/dl

*2 HbAlc: normal values 4.0-6.0%

*3 fat: normal values 50-140 mg/dl

*4 blood pressure: normal values 139-101/89-61 mmHg

[0074]

4

TABLE 4

inspection data

the name
patients
blood pressure

cases
of a disease
sex
age
*1
observation

1
high blood pressure
female
51
182/100
Numerical value became stable without

146/68
oral medicine after drinking for one month.

2
high blood pressure
female
44
130/90
Blood pressure had been barely kept

and cerebrovascular

120/85
in the range between 90 to 130 by

infarction

taking three kinds of blood pressure

descending agents (Ca-antagonist,

ACE inhibitor, β-blocker), but after

drinking for three months, could

stop taking these medicines.

3
high blood pressure
male
60
178/106
Subjective symptoms such as feeling

139/84
of oppression in head, headache,

lumbago, and the like were completely

vanished after drinking for three months.

4
high blood pressure
male
72
141/86
Numerous value became stable by

122/72
drinking for a week.

Top: befor,

Bottom: after

*1 blood pressure: normal values 139-101/89-61 mmHg

[0075]

5

TABLE 5

inspection data

the name of
patients
RBC
WBC
Hb
Ht
BUN
CRP

cases
a disease
sex
age
*1
*2
*3
*4
*5
*6
observation

1
systemic lupus
female
29
3.20 million
9100
7.2
22.8
29
4
Normal numerical value was improved as show in

erythematosus

4.17 million
6300
11.8
36.2
16.9
0.1
Table after drinking for 12 months.

Top: befor,

Bottom: after

*1 RBC = red blood corpuscles: normal values 3.5 million-4.5 million/mm3

*2 WBC = white blood corpuscles: normal values 4000-9000/mm3

*3 Hb = hemoglobin: normal values 2-15 g/dl

*4 Ht = hematocrit: normal values 36-45% (adult female)

*5 BUN = blood urea nitrogen: normal values 110 U/I (RIA; radioimmunoassay)

*6 CRP: normal values less than 1.0 mg/dl

[0076]

6

TABLE 6

inspection data

the name of
patients
GOT
GPT
γ-GTP
tumor marker
tumor marker

cases
a disease
sex
age
*1
*2
*3
AFP*4
TPA*5
observation

1
cancer of the liver
male
61
53
65
192.4

Since he was diagnosed as

48
28
82.4

liver cancer, he had been treated

by taking anticancer drug. Confirmed

by the checkup that numerical value

had been improved as shown in Table

after drinking three months.

2
cancer of the
male
57
126
73

53.8
321
Progressing viral hepatitis type C →

hepar

57
39

19.3
103
Cirrhosis → liver cancer but the condition

was improved and numerical valued tumor

marker was also improved as shown in

Table.

Top: befor,

Bottom: after

*1 GOT: normal values 5-35 KU/ml

*2 GPT: normal values 5˜25 KU/ml

*3 γ-GTP: normal values less than 40 units (adult)

*4 tumor marker AFP: normal values less than 20 ng/ml (RIA)

*5 tumor marker TPA: normal values less than 110 U/I (RIA)

[0077]

7

TABLE 7

inspection data

the name of
patients
tumor marker
tumor marker
CA125
CA19-9

cases
disease
sex
age
PAP *1
PSA *2
*3
*4
observation

1
cancer of the prostate
male
57
216

tNumerical value was improved as

0.5

shown in Table by drinking for

three months.

2
cancer of the prostate
male
56

3.8

Completely recovered by

0.8

drinking for two months.

3
ovarian cancer
female
77

3000

Tumor having a size of about 5 cm

7

had reduced to about 1 cm after

drinking for six months, and

numerical value was improved

after one year as shown in Table.

4
cancer of the colon
male
82

42.3
Numerical value was improved as

32.1
shown in Table by drinking

for two months.

Top:befor,

Bottom: after

*1 tumor marker PAP: normal values less than 3.0 ng/ml (RIA)

*2 tumor marker PSA: normal values less than 3.0 ng/ml (RIA)

*3 CA125: normal values less than 50 U/ml

*4 CA19-9: normal values less than 37 U/ml

[0078]

8

TABLE 8

inspection data

the name of
patients
WBC
white blood
blood
protein M
CA 125
CA 19-9

cases
a disease
sex
age
*1
corpuscles *2
platelets *3
*4
*5
*6
observation

1
acute myelocytic
female
45
1800

Headache, stiff shoulders, nausea,

leukemia

3700

dorsalgia, constipation, halitosis

and the like wholly vanished, and

physical condition was also

improved after drinking for

three months.

2
myelocytic leukemia
male
59

6000
6100

Numerical value was improved as

9400
177000

shown in Table after drinking

for 10 days.

3
multiple myeloma
female
64

2200
30000
10080

Numerical values of leukocyte and

3600
80000
2120

thrombocyte were improved to

reach certainly to normal numerical

values although present numerical

values were still lower than normal

numerical values after drinking for

three months.

4
aplastic anemia
female
34

2360
1060
First, it was doubiful that this

38
87
anemia was malignant by the

inspection but numerical value was

improved as shown in Table after

drinking for three months, and it

was confirmed that this anemia

was benign.

Top: befor,

Bottom: after

*1WBC: normal values 4000-9000/mm3

*2 white blood corpuscles: normal values 4000-9000/mm3

*3 blood platelets: normal values 0.2 million-0.4 million/mm3

*4 protein M: normal values 1700/mm3

*5 CA125: normal values less than 50 U/ml

*6 CA19-9: normal values less than 37 U/ml

[0079]

9

TABLE 9

inspection data

the name of
patients
GOT
GPT
γ-GTP
ZTT
TTT

cases
a disease
sex
age
*1
*2
*3
*4
*5
observation

1
hepatitis
female
53
73
97

There was no effect by medication of

34
33

two kinds of herbal medicines

but improved after drinking for

one month.

2
chronic viral hepatitis
male
50
86
140

Numerical value was completely

type B

30
14

improved after drinking for

three months.

3
viral hepatitis type B
male
50
86
40

Numerical value was completely

30
14

improved after drinking for

one month.

4
viral hepatitis type C
female
45
88
155
87
12.6

Function of liver began

83
143
75
14

to be improved by drinking for

one month.

5
viral hepatitis type C
male
49
307
410
70
33.6
17.4
Usually feeling overworked by

216
290
52
33.1
15.6
hard work but improved by drinking

for two months.

6
chronic viral hepatitis
female
55
85
147
36
13.1
3.7
Numerical value was improved

type C

57
79
19
12
3.5
as shown in Table. When drinking

was stopped, condition changed

for the worse. It has been improved

and stable since she started to

drink again.

7
chronic viral hepatitis
male
65
85
210

Quantitative-qualitative analysis

type B

14
8

reaction of antigen of virus type C

of hepatitis became minus by

drinking for one year.

Top: befor,

Bottom: after

*1 GOT: normal values 5-35 KU/ml

*2 GPT: normal values 5-25 KU/ml

*3 γ-GTP: normal values less than 40 units (adult)

*4 ZZT: normal values 2-14 units

*5 TTT: normal values 0-5 units

[0080]

10

TABLE 10

inspection data

the name of
patients
GOT
GPT
γ-GTP
ZTT
TTT

cases
a disease
sex
age
*1
*2
*3
*4
*5
observation

8
viral hepatitis type C
female
40
68
60

Normal numerical value was improved to

18
12

normal value after drinking for two months.

9
chronic viral hepatitis
male
51
88
122

Body condition and numerical

type C

48
69

value were improved as shown in

Table after drinking for 12 months.

10
chronic hepatitis
male
51
85
147

Numerical value was improved

57
79

as shown in Table after

drinking four months.

Top: befor,

Bottom: after

*1 GOT: normal values 5-35 KU/ml

*2 GPT: normal values 5-25 KU/ml

*3 γ-GTP: normal values less than 40 units (adult)

*4 ZZT: normal values 2-14 units

*5 TTT: normal values 0-5 units

[0081]

11

TABLE 11

inspection data

patients
CRP
RF

cases
the name of a disease
sex
age
*1
*2
observation

1
multiple articular
female
63
1.6
109
Numerical value was improved as shown in Table by drinking for one

rheumatism

0.7
94
month.

2
rheumatism
female
41
1.8

Numerical value was improved as shown in Table, and swelling and

1.1

ache of fingers were also improved after drinking for two months.

Top: befor,

Bottom: after

*1 CRP: normal values less than 1.0 mg/dl

*2 RF = rheumatoid factors: normal values less than 35 U/ml

[0082]

12

TABLE 12

inspection data

patients
Ige-RIST
cat
cedar
house dust
weeds

cases
the name of a disease
sex
age
*1
*2
*3
*4
*5
observation

1
atopic dermatitis
male
39
4628
13.8
41.58
≧100
345
Numerical value was improved as shown in Table,

780
11.5
33.6
70
2.8
and at the same time taking steroid medicine

became not necessary.

2
atopic dermatitis
male
39
476

He had 37 years history of atopic but he can be

332

stopped taking seroid medicine by drinking for

six months.

3
atopic dermatitis
male
23
1671

Condition was much improved and numerical value

1270

was also improved by drinking for five months.

Top: befor,

Bottom: after

*1 Ige-RIST: normal values less than 280 IU/ml

*2 cat: normal values less than 0.34 UA/ml

*3 cedar: normal values less than 0.34 UA/ml

*4 house dust: normal values less than 0.34 UA/ml

*5 weeds: normal values less than 0.34 UA/ml

[0083]

13

TABLE 13

inspection data

patients
MRSA

cases
the name of a disease
sex
age
*1
observation

1
MRSA
female
79
positive
Methicillin-Resistant Staphylococcus Aureus (MRSA) positive

negative
changed to MRSA negative by drinking for three months.

Top: befor,

Bottom: after

*1 MRSA: normal values negative

[0084]

14

TABLE 14

inspection data

patients
triglyceride
obesity index
γ-GTP
amount of urine

cases
the name of a disease
sex
age
*1
*2
*3
*4
observation

1
obesity
female
53
220
+16.4%

Numerical value was improved as shown in

172
+8.9%

Table by drinking for two weeks.

2
emaciation and slight
female
44

−18.6%
87

Numerical value was improved as shown in

hepatopathy

−6.7%
45

Table by drinking for six weeks.

3
chronic nenal
male
42

20-50
Quantity of urine was reduced to numerical

460
value as shown in Table after drinking for

four weeks.

Top: befor,

Bottom: after

*1 triglyceride: normal values 50-140 mg/dl

*2 obesity index: normal values −10-+10%

*3 γ-GTP: normal values less than 40 units

*4 amount of urine: normal values 500-2000 ml/day

EXAMPLE 10

Cosmetics and Hair Restoring

[0085] Bioactivator 4 was applied on the hair of head of five persons for test, and number of fallen hair after washing hair was counted for each person. Average 7 fallen hairs were counted before treatment while average 2 fallen hairs were counted one month after application test.

[0086] Further, said bioactivator 4 was applied on the face, the back of the neck, and the hands of a person for test before playing golf on a fine day in May and no sunburn was recognized after playing golf. Further, said bioactivator was applied on her face everyday and as the result, spots and freckle reduced after one month.

EFFECT OF THE INVENTION

[0087] In the present invention the bioactive effect of iron salt is stabilized by magnesium salt and as the result, the bioactive effect of said iron salt does not degrade and constant stable effect is ensured. Especially the bioactivator of the present invention is useful for medicine to treat incurable disease such as diabetes growth promoting agent of animals and plants.

Number	Date	Country	Kind
2000-168702	Jun 2000	JP
2001-165956	Jun 2001	JP

Biologically active agents and drugs

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