Claims
- 1. A biologically pure culture of the microorganism strain Actinoplanes sarveparensis CBS No. 305.76, said culture being capable of producing antibiotic L 13365 in a recoverable quantity upon aerobic fermentation of assimilable sources of carbon, nitrogen and inorganic salts, said antibiotic having the following characteristics:
- (a) Melting point: 210.degree. C.;
- (b) Approximate elemental composition of 51.35 percent carbon, 5.05 percent hydrogen, 10.50 percent nitrogen, 11.05 percent sulfur and 22.05 percent oxygen;
- (c) Ultraviolet spectrum: characteristic ultraviolet absorption bands in the following
- ______________________________________solvent systemsSolvent .gamma..sub.max (nm) E.sub.1cm.sup.1%______________________________________Hydrochloric acid0.1N 360 103 290 (shoulder) 237 326Buffer 0.15 M 408 (shoulder)pH 7.5 378 67 290 (shoulder) 237 326Buffer 0.15 M 408 99pH 8.8 290 (shoulder) 238 336______________________________________
- (d) Infrared spectrum: characteristic infrared absorption bands in nujol at the following frequencies (cm.sup.-1): 3400 (shoulder), 3350, 3200 (shoulder), 3100 (sharp), 2930 and 2870 (nujol), 2800-2400, 2380 (atmospheric carbon dioxide), 1750 (sharp), 1670 (sharp), 1620 (sharp), 1540 (sharp), 1480 (sharp), 1460 and 1380 (nujol), 1420 (sharp), 1340 (sharp), 1320 (sharp), 1280 (sharp), 1240 (sharp), 1195 (sharp), 1170 (sharp), 1145 (sharp), 1120 (sharp), 1075 (broad), 1015 (sharp), 990 (sharp), 865 (sharp), 840 (sharp), 800 (sharp), 770 (sharp), 755 (sharp), 740 (sharp), 720 (nujol);
- (e) Specific rotation: [.alpha.].sub.436.sup.25 =+125.degree. (C=0.8 percent in methanol:chloroform 1:1);
- (f) Solubility: Soluble in dimethylsulfoxide, dimethylformamide and in chloroform/methanol mixtures. Slightly soluble in chloroform, methanol, methanol/ethyl acetate mixtures, sodium bicarbonate solutions and glacial acetic acid; insoluble in water and in the other common organic solvents;
- (g) Characteristic reactions:
- ______________________________________Fehling positiveTollens positiveKMnO.sub.4 positiveH.sub.2 SO.sub.4 conc. dark brown colorNinhydrin negativeFeCl.sub.3 positiveMillon negativeSchiff negativeAnthrone positiveFolin Ciocalteau negativeElson Morgan negative______________________________________
- (h) Ionizable functions: an ionizable function potentiometrically evidenced with pK.sub.a =7.8 (methylcellosolve:water 4:1);
- (i) R.F. values: chromatography on Whatman paper No. 1; Visualization of the spots by microbiological development on Staphylococcus aureus
- ______________________________________Eluting System Rf value______________________________________Buffer pH 6.0 saturated n-butanol 0.75Water saturated n-butanol + 2% 0.80p-toluenesulfonic acidWater saturated n-butanol + 2% 0.70conc. ammoniaButanol saturated buffer pH 6.0 0.0Ammonium chloride (20% W/V inwater) 0.0n-butanol:methanol:water 40:10:20 0.90containing 0.75 g methyl orangen-butanol:methanol:water 40:10:30 0.90water:acetone 1:1 0.35water saturated ethyl acetate 0.0-0.31Thin layer chromatography on silica gelplates HF/UV.sub.254. Visualization of thefluorescent spot under the ultravioletlight.chloroform:methanol 85:15 0.51______________________________________
Priority Claims (1)
Number |
Date |
Country |
Kind |
26934/76 |
Jun 1976 |
GBX |
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Parent Case Info
This is a division of application Ser. No. 802,215 filed May 31, 1977, now U.S. Pat. No. 4,100,273.
US Referenced Citations (5)
Divisions (1)
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Number |
Date |
Country |
Parent |
802215 |
May 1977 |
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