Claims
- 1. A blotting composition, comprising a substrate applied to an activated microporous membrane comprising a membrane polymer and having (1) an internal surface comprising interstices or pore surfaces and (2) an external surface;
- wherein substantially all said surfaces of the microporous membrane are coated without significant chemical reaction to substantially all said available surface areas of said microporous membrane and thereby activated by a polyaldehyde-containing mixture in an amount sufficient to effectively activate the microporous membrane; and
- wherein the mixture is soluble enough to penetrate substantially all said interstices of the microporous membrane without substantial damage to the pore structure.
- 2. The blotting composition as recited in claim 1, wherein the substrate is a gel.
- 3. The blotting composition as recited in claim 1, wherein the substrate is a gel comprising macromolecules selected from the group consisting of DNA, RNA, and proteins.
- 4. The blotting composition as recited in claim 1, wherein:
- the substrate is a gel which comprises macromolecules electrophoretically separated into a series of bands; and
- the gel additionally comprises a dye that binds to the macromolecules.
- 5. The blotting composition as recited in claim 1 wherein the substrate comprises materials capable of being bound to the polyaldehyde-containing mixture.
- 6. The blotting composition as recited in claim 5 wherein said materials comprise amine-containing materials.
Parent Case Info
This is a continuation-in-part application of copending application, Ser. No. 321,282, filed on Mar. 9, 1989, which is a divisional application of Ser. No. 096,730, filed on Sept. 14, 1987, now U.S. Pat. No. 4,824,870 issued Apr. 25, 1989.
US Referenced Citations (17)
Foreign Referenced Citations (1)
Number |
Date |
Country |
54131995 |
Oct 1979 |
JPX |
Non-Patent Literature Citations (3)
Entry |
Y. Chen, S. Mason & R. E. Sparks, "Collagenase Immobilized on Cellulose Acetate Membranes", Biomaterial: Interfacial Phenomena and Applications, pp. 484-491, 1982. |
Shlomo Margel, "Polyacrolein Microspheres", Methods in Enzymology, vol. 112, 1985, pp. 164-175. |
"Soluble-Insoluble Complex of Trypsin Immobilized on Acrolein-Acrylic Acid Copolymer", Biotechnology and Bioengineering, vol. XVIII, (1976). |
Divisions (1)
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Number |
Date |
Country |
Parent |
96730 |
Sep 1987 |
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Continuation in Parts (1)
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Number |
Date |
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Parent |
321282 |
Mar 1989 |
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