Claims
- 1. A biologically pure culture of the microorganism Steptomyces hygroscopias having all of the identifying characteristics of ATCC 53653, said culture being capable of producing the compound BMY-40800 having the following characteristics:
- (a) a white crystalline solid containing the elements carbon, hydrogen, oxygen, and nitrogen in approximately the following percentages: C, 58.16; H, 6.94; N, 12.84; and O (by difference), 22.26;
- (b) an infrared absorption spectrum (KBr) substantially as shown in FIG. 1;
- (c) a molecular formula of C.sub.72 H.sub.104 N.sub.14 O.sub.20 ;
- (d) a 360 MHz proton magnetic resonance spectrum in CDCl.sub.3 substantially as shown in FIG. 3;
- (e) a 90.5 MHz .sup.13 C magnetic resonance spectrum in CDCl.sub.3 substantially as shown in FIG. 3;
- (f) exhibits an ultraviolet absorption maximum and absorptivity when dissolved in methanol at a concentration of 0.01538 g/l of 286 nm (a=0.2891);
- (g) an apparent molecular weight by FAB mass spectrometry of about 1484;
- (h) exhibits in silica gel thin layer chromatography an Rf value of 0.35 with the solvent system chloroform:methanol (95:5 v/v);
- (i) exhibits a high performance liquid chromatography retention time of 15 of minutes and a capacity factor K'=8.9 with a C.sub.18 reversed phase silica gel column and the solvent system acetonitrile:tetrahydrofuran:water (4:1:5 v/v); and
- (j) is effective in inhibiting the growth of P388 leukemia in mice;
- in a recoverable quantity upon aerobic cultivation in an aqueous nutrient medium containing assimilable sources of carbon and nitrogen.
Parent Case Info
This application is a division of co-pending application Ser. No. 155,778, filed Feb. 16, 1988, now U.S. Pat. No. 4,994,271.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
3875006 |
Belloc et al. |
Apr 1975 |
|
4415669 |
Hernandez |
Nov 1983 |
|
4504580 |
Hanada et al. |
Mar 1985 |
|
Non-Patent Literature Citations (1)
Entry |
Bergey's Manual of Determinative Bacteriology, 8th ed. 1974. |
Divisions (1)
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Number |
Date |
Country |
Parent |
155778 |
Feb 1988 |
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