The present invention relates, in general, to thermally insulated transport containers and, in particular, to such containers in which spermatozoa semen can be maintained motile and fertile and, thus, render it possible to effectively transport this type of material over long distances.
Transporting semen (germplasm), for example equine or canine semen, is beneficial to breeders for several reasons. Shipping semen is less costly than transporting female horses or dogs to an unfamiliar facility. Stress and risk of disease are minimized when female horses or dogs can remain at home. Further, using artificial insemination allows a stallion to service more mares than he could using natural service and allows the stallion to continue to show or perform during the breeding season. When shipped semen is handled properly, pregnancy rates approach those achieved using natural service.
Semen destined for transport is collected from a male, examined, and cooled for shipment. The development of conventional systems allowing controlled cooling has been instrumental. The advantages of using cooled semen make it a valuable addition to breeding programs and has been used to increase the genetic pool in many breeds.
However, numerous factors influence pregnancy rates achieved when mares are bred with transported cooled stallion semen. For instance, sperm are very sensitive to many environmental factors, including temperature, light, physical trauma, and a variety of chemicals. Any factor that impacts the ability of sperm to resist environmentally-induced damage will adversely affect fertility achieved when using cooled transported semen. Semen must be handled from collection to insemination in such a manner as to not shock nor damage the sperm. If collection or storage devices are contaminated by bacteria, chemicals, or even soap residue, the survivability of the sperm cells can be severely diminished. Proper temperature control of semen prior to cooling and prior to insemination is crucial. For example, if semen is initially mixed with extender that is too cool or too warm, damage will likely occur.
Mares will ovulate 24 to 48 hours before the end of heat and pregnancy rates from cooled stallion semen are the highest when mares are inseminated within 24 hours following semen collection. Some stallion semen is still highly viable up to 48 hours following collection. Semen transported and stored for up to 72 hours may appear to have good motility, but fertilization capabilities are typically poor. Thus, when it is required to transport equine semen samples over long distances or even overseas, it is typically necessary to maintain the motility and fertility of the spermatozoa for 48 hours, at the very minimum, and ideally for more than 72 hours, in order that a sample reaches its destination and can be effectively used.
Although semen specimens can be transported for such prolonged periods of time if special motorized refrigeration units are used, the costs of such apparatus and the weight penalties incurred when air mail/freight is involved, are excessive. Thus, there has been an ongoing desire for improved, inexpensive, and disposable containers that are self-contained, passively cooled, and sufficiently light to enable ready dispatch by conventional delivery/mail services.
Spermatozoa from most animal species are susceptible to irreversible damage if exposed to a sudden drop in temperature, also known as “cold shock.” It is known that stallion spermatozoa are more susceptible to cold shock than bovine, ovine, or porcine spermatozoa. Cold shock is generally considered to be the result of rapid cooling from 20 degree C. to 8 degree C. It is known that semen can be cooled relatively quickly from about 37 degree C. (99 degree. F.) down to about 20 degree C., but must be slow cooled at a rate of 0.05 C. degree/min from 20 degree C. to 5.degree. C. (47 degree. F.).
Establishing and maintaining the proper cooling rate of the refrigerant/coolant to which the semen is exposed is among the main requirements of a bodily fluid transporting unit. Also of great interest are toxicity of the refrigerant/coolant and the exposure of the semen to moisture that may cause certain types of microorganisms to spawn, so that maintenance of aseptic conditions can very difficult. In addition, maintaining the motility and fertility of the transported spermatozoa for at least 48 hours is required.
A bodily fluid transporting unit, constructed in accordance with the present invention, includes a container having a chamber and a syringe housing and a refrigerant pack in the chamber of the container. The syringe housing has a passage that extends along the length of the syringe housing and is defined by resilient walls. The syringe is friction-fit in the passage of the syringe housing. A bodily fluid transporting unit, constructed in accordance with the present invention, also includes a lid so mated with the container and closing the chamber of the container that the chamber of the container is air-tight and moisture-tight.
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Container 30 has a chamber 32 into which refrigerant pack 50 and a syringe housing are placed. Top surface 30a of container 30 has a ridge 30b circumferentially surrounding chamber 32 of the container 30 and a groove 30c circumferentially surrounding ridge 30b. The outer edges of top surface 30a of container 30 surround groove 30c of the container 30. Container 30 is preferably made from a rigid foamed plastic.
Lid 40 has a bottom surface 40a that has a ridge 40b that is sized and located to extend into groove 30c in container 30. Lid 40 also has a step 40c circumferentially surrounding ridge 40b and bears against top surface 30a of container 30. Ridge 30b of container 30 is sized and located to fit tightly against the inside surface of ridge 40b of lid 40 after refrigerant pack 50 and a syringe housing are placed in the container 30 to close the container 30 with the lid 40. The mating of container 30 and lid 40 results in chamber 32 of the container 30 being air-tight and moisture-tight. Lid 40 is preferably made from a rigid foamed plastic. A syringe 42 is shown in dashed lines in
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Syringe housings 70 (
It is important that syringes and test vials stored in a syringe housing remain in place while the bodily fluid transporting unit is moved from one location to another. Consequently, the syringe housing should be designed to assure that syringes and test vials do not fall out of the syringe housing or are not displaced in the syringe housing when the bodily fluid transporting unit is exposed to commonly expected hazards, such as being dropped by a handler or displaced in a vehicle carrying the bodily fluid transporting unit.
In accordance with the present invention, syringes and test vials are friction-fit in the passages in the syringe housings, so that the resilient walls that define the passages bear against to syringes and the test vials. To enhance retention of syringes and test vials in syringe housings constructed in accordance with the present invention, the syringe housings preferably include one or both:
The foregoing illustrates some of the possibilities for practicing the invention. Many other embodiments are possible within the scope and spirit of the invention. It is, therefore, intended that the foregoing description be regarded as illustrative rather than limiting and that the scope of the invention is given by the appended claims together with their full range of equivalents.
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Number | Date | Country | |
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20200085556 A1 | Mar 2020 | US |