Patent Grant
10188671
The present invention relates to medicaments for preventing or treating diseases involving a pathogenic amyloid.
Usually, proteins are each folded to form the specific native structure thereof, thereby exerting biological functions. When the proteins are misfolded, however, they may aggregate to form β-sheet-rich fibers (i.e., to form amyloid). Aggregates (e.g., oligomers, proto-fibrils, fibers) produced through this amyloid formation are known to cause various dysfunctions (these disorders are generally referred to as “amyloid diseases”). Here, 20 or more proteins have been identified as substances responsible for the amyloid diseases. Example of such amyloid known include: amyloid β, tau protein in Alzheimer's disease; α-synuclein in Parkinson's disease; amylin in diabetes mellitus; transthyretin in systemic amyloidosis; and huntingtin in Huntington's disease.
For example, as for amyloid β (abbreviated to Aβ), which is a causative amyloid of Alzheimer's disease, inhibitors of enzymes mediating the production of Aβ from a precursor protein, promoters of Aβ-degrading enzymes, immunotherapy, Aβ aggregation inhibitors, and the like are known as strategies to develop therapeutic drugs targeting these pathogenic amyloids.
On the other hand, it has been reported as to Aβ that a Met-oxidized form of an Aβ peptide (the sulfur atom of the Met residue is oxidized to sulfoxide (—S═O—)) remains in a small amount in vivo, and the Met-oxidized form is less aggregable as compared with the native Aβ peptide (Non Patent Literatures 1 to 3). From these viewpoints, the present inventors reported that oxidized forms of Aβ peptides are obtained by the oxidation of the Aβ peptides using a flavin photocatalyst having an Aβ-binding site represented by the formula (a), and these oxidized forms of Aβ peptides suppress the aggregation of Aβ (Non Patent Literature 4).
The flavin photocatalyst used in Non Patent Literature 4 described above, however, has oxidative activity even in the absence of Aβ. This flavin photocatalyst might therefore react nonspecifically in vivo and has thus been difficult to apply in vivo, though applicable in vitro. In addition, the flavin photocatalyst could be applied only to Aβ peptides.
Thus, an object of the present invention is to provide: a compound useful as an amyloid oxidation catalyst which is applicable in vivo, is selective to amyloids and is applicable not only to Aβ peptides but to other amyloids; and a prophylactic or therapeutic drug for an amyloid-related disease, comprising the same.
Accordingly, the present inventors conducted various studies to develop a catalyst which has selective oxidative activity against amyloids and is applicable in vivo, and consequently completed the present invention by finding that a boron-dipyrrin complex, which is represented by the following formula (1), having a halogen atom or a halogenoalkyl group on the boron atom exhibits strong oxygenation activity on an Aβ peptide and other amyloids; the complex exhibits particularly strong oxygenation activity on very toxic Aβ peptide aggregates; the complex exhibits little oxygenation activity on peptides other than amyloid; and the complex is highly stable in water and/or under light irradiation, and that the boron-dipyrrin complex represented by the following formula (1) is useful as an in vivo catalyst which yields oxidized forms of amyloids having no aggregability. Further, a portion of the boron-dipyrrin complex represented by the following formula (1) (wherein R1 is hydrogen or alkyl) emits strong fluorescence upon light irradiation and is very stable in water and/or under light irradiation conditions. Thus, the portion is found to be useful as a fluorescent dye in vivo and/or in tissues or as an intermediate for the manufacture of the compound represented by formula (1). In this way, the present inventors have completed the present invention.
Specifically, the present invention provides the following items [1] to [13].
[1] A boron-dipyrrin complex represented by the following formula (1):
wherein X1 and X2 are the same or different and each represent a halogenoalkyl group or a halogen atom;
R1 represents a hydrogen atom, an alkyl group, or a group represented by formula (b):
R2 and R6 are the same or different and each represent a hydrogen atom or a halogen atom;
R3, R4, R5, and R7 are the same or different and each represent a hydrogen atom, a halogen atom, or an alkyl group;
R8 represents a hydrogen atom or —(CH2)l—(Y)m—(CH2)n—Z wherein Y represents —CO—, —COHN—, or a triazole ring, Z represents a carboxyl group, a sulfonic acid group, or a —CO-peptide residue, l and n each represent an integer of 1 to 6, and m represents 0 or 1;
R9 and R10 are the same or different and each represent a hydrogen atom, an alkyl group, an alkoxy group, a halogen atom, an amino group, a nitro group, or a cyano group; and
R8 and R10 optionally together form an alkylene group.
[2] The boron-dipyrrin complex according to item [1], wherein X1 and X2 are the same or different and are each a fluoro C1-C4 alkyl group or a halogen atom.
[3] The boron-dipyrrin complex according to item [1] or [2], wherein one of R2 and R6 is a halogen atom and the other is a hydrogen atom or a halogen atom.
[4] The boron-dipyrrin complex according to any one of items [1] to [3], wherein R1 is the group represented by formula (b).
[5] A boron-dipyrrin complex represented by the following formula (1A):
wherein X1 and X2 are the same or different and each represent a halogenoalkyl group or a halogen atom;
one of R2 and R6 represents a halogen atom and the other represents a hydrogen atom or a halogen atom;
R3, R4, R5, and R7 are the same or different and each represent a hydrogen atom, a halogen atom, or an alkyl group;
R8 represents a hydrogen atom or —(CH2)l—(Y)m—(CH2)n—Z wherein Y represents —CO—, —CONH—, or a triazole ring, Z represents a carboxyl group, a sulfonic acid group, or a —CO-peptide residue, l and n each represent an integer of 1 to 6, and m represents 0 or 1;
R9 and R10 are the same or different and each represent a hydrogen atom, an alkyl group, an alkoxy group, a halogen atom, an amino group, a nitro group, or a cyano group; and
R8 and R10 together optionally form an alkylene group.
[6] The boron-dipyrrin complex according to item [5], wherein X1 and X2 are the same or different and are each a fluoro C1-C4 alkyl group or a halogen atom.
[7] The boron-dipyrrin complex according to item [5] or [6], wherein one of R2 and R6 is a halogen atom and the other is a hydrogen atom or a halogen atom.
[8] A medicament comprising, as an active ingredient, the boron-dipyrrin complex according to any one of items [5] to [7].
[9] The medicament according to item [8], which is a drug for preventing or treating a disease involving a pathogenic amyloid.
[10] A pharmaceutical composition comprising: the boron-dipyrrin complex according to any one of items [5] to [7]; and a pharmaceutically acceptable carrier.
[11] Use of the boron-dipyrrin complex according to any one of items [5] to [7] for the manufacture of a drug for preventing or treating a disease involving a pathogenic amyloid.
[12] The boron-dipyrrin complex according to any one of items [5] to [7] for preventing or treating a disease involving a pathogenic amyloid.
[13] A method for preventing or treating a disease involving a pathogenic amyloid, comprising administering an effective amount of the boron-dipyrrin complex according to any one of items [5] to [7].
A boron-dipyrrin complex (1A) of the present invention has increased catalytic activity in which a pathogenic amyloid such as an Aβ peptide is oxygenated. The complex can inhibit amyloid aggregation through amyloid oxygenation in vivo. In addition, the complex has increased oxygenation activity on aggregated amyloid. The complex exhibits little oxygenation activity on peptides other than amyloid. Further, the complex is very stable in water and/or under light irradiation conditions. Thus, the complex is useful as a drug for preventing or treating a disease involving a pathogenic amyloid. Moreover, a compound represented by formula (1) wherein R1 is a hydrogen atom or an alkyl group is useful as a fluorescent dye for in vivo and/or tissue staining. As used herein, the term “oxygenation” means, among oxidation reactions, a specific reaction in which an oxygen atom is bonded.
In formula (1), X1 and X2 are the same or different and each represent a halogenoalkyl group or a halogen atom. As the halogenoalkyl group, a halogeno C1-C4 alkyl group is preferable, and a fluoro C1-C4 alkyl group is particularly preferable. Specific examples of the more preferable halogenoalkyl group include perfluoro C1-C4 alkyl groups such as a trifluoromethyl group, a pentafluoroethyl group, and a heptafluoropropyl group. Examples of the halogen atom include a fluorine atom, a chlorine atom, a bromine atom, and an iodine atom, and a fluorine atom is preferable.
X1 and X1 may both be halogen atoms or halogenoalkyl groups, but preferably, X1 is a halogenoalkyl group and X2 is a halogen atom; more preferably, X1 is a perfluoro C1-C4 alkyl group and X2 is a fluorine atom; and further preferably, X1 is a trifluoromethyl group or a pentafluoroethyl group and X2 is a fluorine atom.
In formula (1), R1 represents a hydrogen atom, an alkyl group, or a group represented by formula (b):
In formula (b), R8 represents a hydrogen atom or —(CH2)l—(Y)m—(CH2)n—Z wherein Y represents —CO—, —COHN—, or a triazole ring, Z represents a carboxyl group, a sulfonic acid group, or a —CO-peptide residue, l and n each represent an integer of 1 to 6, and m represents 0 or 1;
R9 and R10 are the same or different and each represent a hydrogen atom, an alkyl group, an alkoxy group, a halogen atom, an amino group, a nitro group, or a cyano group; and
R8 and R10 together optionally form an alkylene group.
The alkyl group represented by R1 is preferably a C1-C6 alkyl group. Specific examples include linear or branched C1-C4 alkyl groups such as a methyl group, an ethyl group, a n-propyl group, an isopropyl group, a n-butyl group, an isobutyl group, and a tert-butyl group. Among them, a methyl group is particularly preferable.
R2 and R6 are the same or different and each represent a hydrogen atom or a halogen atom. Preferably, one of R2 and R6 represents a halogen atom and the other represents a hydrogen atom or a halogen atom. Here, examples of the halogen atom include a fluorine atom, a chlorine atom, a bromine atom, and an iodine atom, and a bromine atom or an iodine atom is more preferable, and an iodine atom is further preferable.
At least one of R2 and R6 may be a halogen atom. In this case, irradiation of light with a long wavelength of 640 nm or more causes strong oxygenation activity on amyloid, in particular, aggregated amyloid. Stability to water and stability under light irradiation conditions are both good.
R3, R4, R5, and R7 are the same or different and each represent a hydrogen atom, a halogen atom, or an alkyl group. Here, examples of the halogen atom include a fluorine, chlorine, bromine, and iodine atom. Examples of the alkyl group include a C1-C12 alkyl group, and a C1-C6 alkyl group is preferable. Examples of the alkyl group include linear or branched alkyl groups such as a methyl group, an ethyl group, a n-propyl group, an isopropyl group, and a n-butyl group.
R3, R5, and R7 are each preferably a hydrogen atom, a halogen atom, or a C1-C6 alkyl group, and more preferably a hydrogen atom or a C1-C6 alkyl group. In addition, R4 is preferably a hydrogen atom. Notably, it is preferable that R1 and R7 not be hydrogen atoms simultaneously.
R8 represents a hydrogen atom or —(CH2)l—(Y)m—(CH2)n—Z. Here, Y represents —CO—, —COHN—, or a triazole ring. Examples of the triazole ring include a 1,2,3-triazole-1,4-diyl group and a 1,2,4-triazole-1,3-diyl group. Z represents a carboxyl group (—COOH), a sulfonic acid group (—SO3H), or a —CO-peptide residue. Examples of the peptide of the —CO-peptide residue include oligopeptides, for example from a dipeptide to a hexapeptide. As the oligopeptides, oligopeptides containing 2 to 6 amino acids selected from Lys, Leu, Val, Phe, and modified amino acids thereof are preferable, oligopeptides containing 4 to 6 amino acids are more preferable, and a pentapeptide is further preferable. Examples of the modified amino acids include amino acids modified by a phenyl group, a halogenophenyl group, a naphthyl group, or the like. A phenyl group-modified amino acid is preferable. Specific examples of the oligopeptides include KLVFF, KLVF(4Ph)F, and KVLF(βPh)F. Here, these preferable oligopeptides exert activity to inhibit aggregation of Aβ. For example, the peptide KLVF(4Ph)F bonded to compound 19 is particularly preferable because of its excellent Aβ aggregation inhibitory activity (Non Patent Literature 4). l and n each represent an integer of 1 to 6, and are each preferably an integer of 2 to 6, m represents 0 or 1.
The alkylene group formed by R8 and R10 bonded together is preferably an alkylene group having 2 to 4 carbon atoms, and examples thereof include an ethylene group, a trimethylene group, and a tetramethylene group. Note that when R8 and R10 taken together form an alkylene group, the tetrahydroquinoline ring is preferably substituted at position 8 by the R10.
R9 and R10 are the same or different and each represent a hydrogen atom, an alkyl group, an alkoxy group, a halogen atom, an amino group, a nitro group, or a cyano group. Here, as the alkyl group, a C1-C6 alkyl group is preferable and examples of the alkyl group include linear or branched C1-C4 alkyl groups such as a methyl group, an ethyl group, a n-propyl group, and an isopropyl group. The alkoxy group is preferably a C1-C6 alkoxy group, and examples thereof include a methoxy group, an ethoxy group, a n-propyloxy group, and isopropyloxy group. Examples of the halogen atom include a fluorine atom, a chlorine atom, a bromine atom, and an iodine atom.
Preferably, in formula (1), X1 and X2 are the same or different and are each a fluoro C1-C4 alkyl group or a halogen atom; R1 is a hydrogen atom, a C1-4 alkyl group, or a group represented by formula (b) (in formula (b), R8 represents —(CH2)l—(Y)m—(CH2)n—Z or R8 and R10 taken together form a C2-C4 alkylene group, and R9 represents a hydrogen atom, a C1-C6 alkyl group, or a C1-C6 alkoxy group); at least one of R2 and R6 is a halogen atom and the other is a hydrogen atom or a halogen atom; and R3, R4, R5, and R7 are the same or different and is each a hydrogen atom, a C1-C6 alkyl group, or a halogen atom.
A compound in which R1 is formula (b) is activated by irradiation of light with a long wavelength of 590 nm or more, has strong oxygenation activity on amyloid such as Aβ, is very stable in water and under light irradiation conditions, and is thus useful as an in vivo catalyst which helps generate nonaggregatable oxygenated amyloid. The compound is represented by the following formula (1A):
(In the formula, X1, X2, R2 to R10 are the same as above.)
A compound in which R1 is a hydrogen atom or an alkyl group emits strong fluorescence and is thus useful as a fluorescent dye which emits fluorescence to stain a living body, tissues, cells, etc. The compound is represented by the following formula (1B):
(In the formula, R1a represents a hydrogen atom or an alkyl group and X1, X2, R2 to R7 are the same as above.)
Preferably, in formula (1A), X1 and X2 are the same or different and are each a fluoro C1-C4 alkyl group or a halogen atom; R8 represents —(CH2)l—(Y)m—(CH2)n—Z or R8 and R10 taken together form a C2-C4 alkylene group, and R9 represents a hydrogen atom, a C1-C6 alkyl group, or a C1-C6 alkoxy group; at least one of R2 and R6 is a halogen atom and the other is a hydrogen atom or a halogen atom; and R3, R4, R5, and R7 are the same or different and are each a hydrogen atom, a C1-6 alkyl group, or a halogen atom.
Preferably, in formula (1B), X1 and X2 are the same or different and are each a fluoro C1-C4 alkyl group or a halogen atom; R1a is a hydrogen atom or a C1-C4 alkyl group; at least one of R2 and R6 is a halogen atom and the other is a hydrogen atom or a halogen atom; and R3, R4, R5, and R7 are the same or different and each represent a hydrogen atom, a C1-C6 alkyl group, or a halogen atom.
When the present compound (1) has an asymmetric carbon atom, an enantiomer may be present and the enantiomer and a racemate are both included in the present invention.
The present compound (1) may be produced in accordance with, for example, the following reaction scheme.
(In the scheme, X1, X2, R4, R5, R8, R9, and R10 are the same as above.)
First, a pyrrole compound (2) is reacted with a triethyl orthoformate compound in the presence of an acid such as trifluoroacetic acid to give a compound (3). For example, acetic acid other than trifluoroacetic acid may be used as the acid. This reaction may be performed in an inert solvent such as dichloromethane, chloroform, or dichloroethane at 0 to 50° C. for 10 min to 10 hours.
Next, the compound (3) is reacted with a halogenoalkyl-modified trihalogenoboron (e.g., KBF3CF3, KBF3C2F5) to give a compound (1B1). In this reaction, the compound (3) is first reacted with an organic amine such as triethylamine in a solvent such as dichloromethane. The resultant was reacted at 0° C. with, for example, an acetonitrile suspension containing a halogenoalkyl-modified trihalogenoboron and a Lewis acid such as trimethylsilyl trifluoromethanesulfonic acid (TMSOTf). After that, the reaction may be conducted at 0° C. to room temperature for 1 to 10 hours.
Then, the compound (1B1) is reacted with a halogen such as iodine to give a compound (1B2). The iodination is preferably performed using an iodination agent such as diacetoxy iodobenzene and iodine. Note that the reaction may be conducted in a polar solvent such as acetonitrile at room temperature for 1 to 10 hours.
After that, the compound (1B2) is reacted with a compound (4) to give a compound (1A). This reaction may be carried out in the presence of an acid such as acetic acid and an organic amine such as piperidine in an inert solvent such as toluene at 100 to 200° C. for 1 to 10 hours. In this reaction, an iodine atom is considered to detach from the boron-dipyrrin ring of the compound (1B2).
Note that the compound (4) may be produced, for example, by performing a Vilsmeier-Haack reaction where a tetrahydroquinoline compound is reacted with dimethylformamide and phosphoryl trichloride to formylate the tetrahydroquinoline compound at position 6.
Regarding a substituent on the dipyrrin structure, various kinds of the substituent of a starting material pyrrole compound may be used for appropriate substitution.
In addition, the compound (1A) wherein R8 is —(CH2)l—CONH—(CH2)m—Z may be obtained by the reaction using H2N(CH2)n—Z and, as a starting material, the compound (4) wherein R8 is —(CH2)l—COOH.
The present compound (1) as so obtained may be isolated and purified from a reaction mixture by using conventional techniques such as washing, crystallization, recrystallization, and chromatography.
The maximum light absorption wavelength of the present compound (1A) is shifted to the long-wavelength side when compared to that of thioflavin T. It was observed that regarding the present compound (1A) placed in the presence of Aβ, the light absorption wavelength was shifted a little more to the long-wavelength side than in the absence of Aβ and the compound exhibited remarkably high fluorescence. These results demonstrate that like thioflavin T, the present compound is bound to Aβ to inhibit an intramolecular rotation, which results in fluorescence emission.
In addition, when the present compound (1A) was added to native Aβ and was irradiated with light with a wavelength of 590 nm or more under physiological conditions, the amount of native Aβ decreased over time so as to increase the amount of oxygenated Aβ to which one to four oxygen atoms were added. The oxygenation efficiency was remarkably higher than that of thioflavin T. Also, the oxygenation reaction catalyzed by the present compound (1A) is selective for Aβ such that its selectivity is very weak with respect to non-amyloid peptides (e.g., angiotensin IV, methionine enkephalin).
Further, the present compound (1A) had higher oxygenation activity on very toxic aggregated peptides than toward Aβ peptide monomers. Furthermore, the present compound (1A) has superior stability to water and stability under light irradiation conditions.
Hence, the present compound (1A) should work as a catalyst which helps selectively oxygenate pathogenic amyloids such as Aβ peptide, amylin, transthyretin, α-synuclein, tau protein, and huntingtin. Once oxygenated, such a pathogenic amyloid cannot form a stacked β-sheet structure, so that its pathogenicity does not occur. Thus, the present compound (1A) is useful as a drug for preventing and/or treating diseases involving a pathogenic amyloid, such as Alzheimer's disease, Parkinson's disease, diabetes mellitus, Huntington's disease, and systemic amyloidosis in animal including a human.
The present compound (1A) catalyzes a reaction in which a pathogenic amyloid is oxygenated. This oxygenation reaction proceeds such that the present compound (1A) is excited by light exposure and is thus activated to oxygenate amyloid. Because of this, when the present compound (1A) is used as a medicament, a patient is preferably irradiated with light after the present compound (1A) is administered. In addition, the light wavelength which enables the present compound (1A) to be under an excited state is a long-wavelength of 590 run or more. Light with this characteristic wavelength is easy to permeate through a living body.
Meanwhile, examples of the amino acid residue in the amyloid that is oxygenated due to the action of the present compound (1A) include a methionine residue having a sulfur atom and a histidine residue having an imidazole ring.
A pharmaceutical composition comprising the present compound (1A) may be prepared, in accordance with a variety of formulation preparation processes, by selecting an appropriate formulation depending on an administration method and by using a pharmaceutically acceptable carrier. Examples of a dosage form of the pharmaceutical composition comprising, as a main ingredient, the present compound (1A) can include oral preparations such as a tablet, a powder, a granule, a capsule, a liquid, a syrup, an elixir, and an oily or aqueous suspension).
An injection may contain a stabilizer, a preservative, and/or a dissolution aid in a preparation. A solution which may contain such pharmaceutical aids may be placed in a container and provided, for example, as a lyophilized solid preparation, which will be prepared upon use. In addition, a single dose may be contained in a container. Also, multiple doses may be contained in a container.
Meanwhile, examples of an external preparation thereof include a liquid, a suspension, an emulsion, an ointment, a gel, a cream, a lotion, a spray, and a patch.
A solid preparation may contain the present compound (1A) and a pharmaceutically acceptable additive. Examples of the additive include fillers, thickeners, binders, disintegrants, solubilization enhancers, wetting agents, and lubricants. These additives may be selected, as needed, and mixed for formulation.
Examples of a liquid preparation thereof include a solution, a suspension, and an emulsion. The liquid preparation may contain, as an additive, a suspending agent and/or an emulsifying agent.
The present compound (1A) may be used as a human medicament. In this case, an adult daily dose ranges from 1 mg to 1 g and preferably from 1 mg to 300 mg.
The following specifically describes the present invention by referring to Examples. The scope of the present invention, however, is not limited to these Examples.
The following experiments were all carried out using a dehydrated solvent under an argon atmosphere.
First, trifluoroacetic acid (1.2 mL, 15.75 mmol) was added dropwise to a dichloromethane solution (35 mL) containing 2,4-dimethyl pyrrole (2.16 mL, 21 mmol) and triethyl orthoformate (1.75 mL, 10.5 mmol). Next, the mixture was stirred at room temperature for 2 hours. Then, the reaction solution was diluted with water and was extracted using dichloromethane to recover an organic layer. The organic layer was washed with saturated saline and dried over anhydrous sodium sulfate. After the organic solvent was removed under reduced pressure, the remainder was dissolved in a small amount of dichloromethane. Subsequently, hexane was added to the resulting solution to yield precipitates. The precipitates were filtered and washed with hexane to give compound 1 as an orange solid (2.73 g, 83%).
1H NMR (CDCl3, 400 MHz): δ 2.32 (s, 6H), 2.49 (s, 6H), 6.14 (s, 2H), 7.05 (s, 1H); 13C NMR (CDCl3, 100 MHz): δ 12.08, 14.23, 117.41, 118.18, 120.13, 146.30, 155.30, 161.39; 19F NMR (CDCl3, 370 MHz): δ −78.10
First, triethylamine (266 μL, 1.91 mmol) was added to a dichloromethane solution (3 mL) containing compound 1 (100 mg, 0.32 mmol). Next, the mixture was stirred at room temperature for 10 min. To the reaction solution was added dropwise a boron trifluoride-diethyl ether complex (353 mL, 2.86 mmol). The mixture was further stirred at room temperature for 1 hour. Then, the reaction solution was diluted with water and was extracted using dichloromethane to recover an organic layer. The organic layer was washed with saturated saline and dried over anhydrous sodium sulfate. After the organic solvent was removed under reduced pressure, the remainder was purified by flash column chromatography (using an eluent ratio of hexane:ethyl acetate=15:1) to give compound 2 as a red solid (70 mg, 89%).
1H NMR (CDCl3, 400 MHz): δ 2.22 (s, 6H), 2.51 (s, 6H), 6.02 (s, 2H), 7.01 (s, 1H); 13C NMR (CDCl3, 100 MHz): δ 11.24, 14.63, 118.96, 120.04, 133.38, 141.17, 156.69; 19F NMR (CDCl3, 370 MHz): δ −149.41
First, an aqueous ammonia solution (28% in water, 1.16 mL, 17.2 mmol) was added to a diethyl ether suspension (150 mL) containing compound 1 (1.35 g, 4.3 mmol). Next, the mixture was stirred at room temperature for 20 min. Then, the reaction solution was diluted with water and was extracted using diethyl ether. An organic layer was dried over anhydrous sodium sulfate and the solvent was removed under reduced pressure to give a solid.
After that, trimethylsilyl trifluoromethanesulfonate (6.2 mL, 34.5 mmol) was added dropwise to an acetonitrile suspension (30 mL) containing ice-cold trifluoro(trifluoromethyl)potassium borate (3.03 g, 4.3 mmol) at 0° C. Next, the mixture was stirred at 0° C. for 20 min. To this reaction solution stirred at 0° C. was added dropwise a solution containing N,N-diisopropylethylamine (2.25 mL, 12.9 mmol) and the above solid which had been stirred at room temperature for 10 min in dichloromethane (110 mL). Subsequently, the temperature of the mixture was raised to room temperature and the mixture was then stirred for 3 hours. After an organic solvent was removed under reduced pressure, the remainder was extracted using dichloromethane to recover an organic layer. The organic layer was washed with water and saturated saline and dried over anhydrous sodium sulfate. After the organic solvent was removed under reduced pressure, the remainder was purified by flash column chromatography (using an eluent ratio of hexane:ethyl acetate=15:1) to give compound 3 as a red solid (737 mg, 58%).
1H NMR (CDCl3, 400 MHz): δ 2.25 (s, 6H), 2.51 (s, 6H), 6.08 (s, 2H), 7.08 (s, 1H); 13C NMR (CDCl3, 100 MHz): δ 11.31, 14.96 (m), 119.76, 120.84, 133.14, 141.71, 157.92; 19F NMR (CDCl3, 370 MHz): δ −185.83 (1F), −76.46 (3F); LRMS (DART): m/z calcd. for C14H16BF4N2+ [M+H]30: 299.1, found: 299.2.
Except that trifluoro(pentafluoroethyl)potassium borate was used, the same procedure as in (compound 3) was repeated to give compound 4 as a red solid (the yield: 23%).
1H NMR (CDCl3, 400 MHz): δ 2.25 (s, 6H), 2.51 (s, 6H), 6.07 (s, 2H), 7.07 (s, 1H); 13C NMR (CDCl3, 100 MHz): δ 11.31, 15.08 (m), 119.77, 133.29, 141.81, 158.34; 19F NMR (CDCl3, 370 MHz): δ −185.18 (1F), −133.76 (2F), −86.44 (3F); LRMS (DART): m/z calcd. for C15H16BF6N2+ [M+H]+: 349.1, found: 349.2.
To an acetonitrile solution (50 ml) containing compound 2 (100 mg, 0.4 mmol) were added iodine (113 mg, 0.44 mmol) and iodobenzene diacetate (143 mg, 0.44 mmol). Next, the mixture was stirred at room temperature for 3 hours. The reaction solution was diluted with a sodium thiosulfate aqueous solution and was then extracted using ethyl acetate to recover an organic layer. The organic layer was washed with water and saturated saline and dried over anhydrous sodium sulfate. After the organic solvent was removed under reduced pressure, the remainder was purified by flash column chromatography (using an eluent ratio of hexane:ethyl acetate=10:1) to give compound 5 as a red solid (167 mg, 84%).
1H NMR (CDCl3, 400 MHz): δ 2.18 (s, 6H), 2.57 (s, 6H), 7.06 (s, 1H); 13C NMR (CDCl3, 100 MHz); δ 13.73, 15.65, 81.99, 120.22, 132.80, 144.34, 157.67; 19F NMR (CDCl3, 370 MHz): δ −148.82. LRMS (ESI−) (m/z) 498.9 [M−1]−1 (calc: 498.9).
Except that compound 3 was used, the same procedure as in Synthesis Example 5 was repeated to give compound 6 as a red solid (the yield: 91%).
1H NMR (CDCl3, 400 MHz): δ 2.24 (s, 6H), 2.58 (s, 6H), 7.16 (s, 1H); 19F NMR (CDCl3, 370 MHz): δ −183.8 (s, 1F), −76.6 (s, 3F). LRMS (ESI−) (m/z) 548.9 [M−1]−1 (calc: 548.9).
Except that compound 4 was used, the same procedure as in Synthesis Example 5 was repeated to give compound 7 (the yield: 77%).
1H NMR (CDCl3, 400 MHz): δ 2.19 (s, 6H), 2.52 (s, 6H), 7.10 (s, 1H); 19F NMR (CDCl3, 370 MHz): δ −215.37 (1F), −166.09 (2F), −118.33 (3F); LRMS (ESI): m/z calcd for C15H12BF6I2N2− [M−H]−1; 598.9, found: 599.0.
First, piperidine (99 μL, 1 mmol) was added to a toluene solution (3 mL) containing compound 2 (25 mg, 0.1 mmol) and 9-julolidine carboxaldehyde (24 mg, 0.12 mmol). Next, the mixture was heated under reflux for 2 hours by using a Dean-Stark apparatus. Then, the reaction solution was cooled to room temperature. After the toluene was removed under reduced pressure, the remainder was purified by flash column chromatography (using an eluent ratio of hexane:ethyl acetate=7:1) to give compound 8 as a blue solid (18 mg, 26%).
1H NMR (CDCl3, 400 MHz): δ 1.94 (m, 4H), 2.22 (s, 3H), 2.25 (s, 3H), 2.54 (s, 3H), 2.74 (t, J=6.27 Hz, 4H), 3.21 (t, J=5.41 Hz, 4H), 5.99 (s, 1H), 6.62 (s, 1H), 6.89 (s, 1H), 7.04 (s, 2H), 7.13 (d, J=15.7 Hz, 1H), 7.31 (d, J=15.7 Hz, 1H); 19F NMR (CDCl3, 370 MHz): δ −145.86. LRMS (ESI+) (m/z) 431.8 (M+) (calc: 432.2).
Except that compound 2 and 4-(dimethylamino)benzaldehyde were used, the same procedure as in Synthesis Example 8 was repeated to give compound 9 (the yield: 51%).
1H NMR (CDCl3, 400 MHz): δ 2.23 (s, 3H), 2.26 (s, 3H), 2.54 (s, 3H), 3.01 (s, 6H), 6.01 (s, 1H), 6.64-6.67 (br, 3H), 6.93 (s, 1H), 7.21 (d, J=16.1 Hz, 1H), 7.40 (d, J=16.1 Hz, 1H), 7.48 (d, J=8.97 Hz); 19F NMR (CDCl3, 370 MHz): δ −142.83; LRMS (ESI): m/z calcd for C22H25BF2N3+ [M+H]+: 380.2, found: 380.1.
Except that compound 5 was used, the same procedure as in Synthesis Example 8 was repeated to give compound 10 as a blue solid (the yield: 19%).
1H NMR (CDCl3, 400 MHz): δ 1.94 (m, 4H), 2.22 (s, 3H), 2.25 (s, 3H), 2.54 (s, 3H), 2.74 (s, 4H, broad), 3.21 (s, 4H, broad), 5.99 (s, 1H), 6.62 (s, 1H), 6.89 (s, 1H), 7.04 (s, 2H), 7.13 (d, J=16.1 Hz, 1H), 7.30 (d, J=16.1 Hz, 1H); 19F NMR (CDCl3, 370 MHz): δ −145.95.
The same procedure as in Synthesis Example 8 was repeated to produce, from compound 3, compound 11 as a blue solid (the yield: 40%).
1H NMR (CDCl3, 400 MHz): δ 1.94 (m, 4H), 2.24 (s, 3H), 2.27 (s, 3H), 2.53 (s, 3H), 2.74 (t, J=6.27 Hz, 4H), 3.21 (t, J=5.41 Hz, 4H), 6.04 (s, 1H), 6.69 (s, 1H), 6.94 (s, 1H), 7.15 (d, J=15.7 Hz, 1H), 7.35 (d, J=15.7 Hz, 1H); 19F NMR (CDCl3, 370 MHz): δ −182.48 (s, 1H), −76.32 (s, 3H). LRMS (ESI+) (m/z) 481.8 (M+) (calc: 482.2).
The same procedure as in Synthesis Example 8 was repeated to produce, from compound 5, compound 12 as a blue solid (the yield: 43%).
1H NMR (CDCl3, 400 MHz): δ 1.9451 (m, 4H), 2.21 (s, 3H), 2.27 (s, 3H), 2.58 (s, 3H), 2.74 (t, J=5.80 Hz, 4H), 3.23 (t, J=5.83 Hz, 4H), 6.74 (s, 1H), 6.93 (s, 1H), 7.03 (s, 2H), 7.22 (d, J=15.7 Hz, 1H), 7.36 (d, J=15.7 Hz, 1H); 19F NMR (CDCl3, 370 MHz): δ −181.43 (s, 1H), −76.34 (s, 3H).
Except that compound 5 and 6-formyl-1,2,3,4-tetrahydroquinoline-hexanoic acid were used, the same synthesis procedure as in Synthesis Example 8 was repeated to give compound 13 (the yield: 39%).
1H NMR (acetone-d6, 400 MHz): δ 1.31 (m, 2H), 1.50-1.57 (m, 4H), 1.82 (m, 2H), 2.10 (s, 3H), 2.16-2.21 (m, 5H), 2.40 (s, 3H), 2.66 (t, J=6.27, 2H), 3.25-3.32 (m, 4H), 6.56 (d, J=8.50, 1H), 6.83 (s, 1H), 7.12 (s, 1H), 7.18-7.22 (m, 2H), 7.29 (s, 1H), 7.40 (d, J=16.1 Hz, 1H); 19F NMR (acetone-d6, 370 MHz): δ −177.2; LRMS (ESI): m/z calcd for C29H34BF2IN3O2+ [M+H]+: 632.2, found: 632.2.
Except that compound 6 and 6-formyl-1,2,3,4-tetrahydroquinoline-hexanoic acid were used, the same synthesis procedure as in Synthesis Example 8 was repeated to give compound 14 (the yield: 23%).
1H NMR (acetone-d6, 400 MHz): δ 1.30 (m, 2H), 1.47-1.58 (m, 4H), 1.81 (m, 2H), 2.12 (s, 3H), 2.17-2.22 (m, 5H), 2.43 (s, 3H), 2.65 (t, J=6.28 Hz, 2H), 3.25-3.32 (m, 4H), 6.56 (d, J=8.54, 1H), 6.93 (s, 1H), 7.10 (s, 1H), 7.19-7.27 (m, 2H), 7.37 (s, 1H), 7.43 (d, J=15.7 Hz, 1H); 19F NMR (acetone-d6, 370 MHz): δ −178a.6 (1F), −73.67 (3F); LRMS (ESI): m/z calcd for C30H34BF4IN3O2+ [M+H]+: 682.2, found: 682.5.
Except that compound 7 and 6-formyl-1,2,3,4-tetrahydroquinoline-hexanoic acid were used, the same synthesis procedure as in Synthesis Example 8 was repeated to give compound 15 (the yield: 13%).
1H NMR (acetone-d6, 400 MHz): δ 1.31 (m, 2H), 1.50-1.57 (m, 4H), 1.82 (m, 2H), 2.12 (s, 3H), 2.15-2.22 (m, 5H), 2.43 (s, 3H), 2.65 (t, J=6.28, 2H), 3.25-3.32 (m, 4H), 6.57 (d, J=8.54, 1H), 6.93 (s, 1H), 7.10 (s, 1H), 7.20 (d, J=8.54, 1H), 7.28 (d, J=15.7 Hz, 1H), 7.37 (s, 1H), 7.43 (d, J=15.7 Hz, 1H); 19F NMR (acetone-d6, 370 MHz): δ −177.9 (1F), −131.2 (2F), −84.03 (3F); LRMS (ESI): m/z calcd for C31H34BF6IN3O2+ [M+H]+: 732.2, found: 731.1.
To DMF (2 mL) in which compound 15 (10.5 mg, 0.015 mmol) and taurine (40 mg, 0.32 mmol) had been dissolved were added 1-ethyl-3-(3-dimethylamino)propyl)carbodiimide (EDC) (15 mg, 0.078 mmol), N,N-diisopropylethylamine (DIPEA) (13 μL, 0.075 mmol), and 1-hydroxybenzotriazole (HOBt.H2O) (10.5 mg, 0.078 mmol). Next, the reaction mixture was stirred overnight at room temperature. The reaction mixture was purified by HPLC to give compound 16 as a dark blue solid.
The yield (2.1 mg, 17%). LRMS (ESI): m/z calcd for C32H39BF4IN4O4S+ [M+H]+: 789.2, found: 789.4; HPLC:tR=36.8 min (based on HPLC analysis at 230 nm with a linear gradient of 0-100% MeCN in 0.1% aq. TFA over 40 min).
To an acetonitrile suspension (10 mL) containing sodium iodide (6.4 g, 38.2 mmol) and sodium carbonate (4.4 g, 31.8 mmol) were added 1,2,3,4-tetrahydroquinoline (2.0 mL, 15.9 mmol) and methyl 6-bromohexanoate (3.0 mL, 19.1 mmol). The reaction solution was heated under reflux for 48 hours. Next, the reaction solution was cooled to room temperature. Subsequently, the reaction mixture was filtered and the organic solvent was removed under reduced pressure. The remainder was diluted with water and then extracted using ethyl acetate. The organic layer was washed with saturated saline and dried over anhydrous sodium sulfate. After the organic solvent was removed under reduced pressure, the remainder was purified by silica gel column chromatography (using an eluent ratio of hexane:ethyl acetate=10:1) to give 1,2,3,4-tetrahydroquinoline-methyl hexanoate as a pale yellow oily substance (3.36 g, 81%).
1H NMR (CDCl3, 400 MHz): δ 1.37 (m, 2H), 1.57-1.74 (m, 4H), 1.94 (m, 2H), 2.33 (t, J=7.48, 2H), 2.75 (t, J=6.74, 2H), 3.17-3.28 (m, 4H), 3.67 (s, 3H), 6.53-6.56 (m, 2H), 6.93 (d, J=5.96, 1H), 7.04 (t, J=7.48, 1H); LRMS (ESI): m/z calcd for C16H24NO2+ [M+H]+: 262.2, found: 262.2.
To dichloromethane (15 mL) in which 1,2,3,4-tetrahydroquinoline-methyl hexanoate (2.25 g, 8.6 mmol) had been dissolved was added phosphoryl chloride (2.4 mL, 25.8 mmol) at 0° C. Next, DMF (6.7 mL, 86 mmol) was added slowly dropwise thereto at 0° C. Then, the temperature of the reaction mixture was raised to room temperature and the reaction mixture was stirred for 2 hours.
Subsequently, the reaction mixture was diluted with water, and aqueous sodium hydroxide at 0° C. was added thereto to bring the pH to 7. After the solvent was removed under reduced pressure, the remainder was extracted using ethyl acetate. The organic layer was washed with saturated saline and dried over anhydrous sodium sulfate. After the organic solvent was removed under reduced pressure, the remainder was purified by silica gel column chromatography (using an eluent ratio of hexane:ethyl acetate=3:1) to give 6-formyl-1,2,3,4-tetrahydroquinoline-methyl hexanoate as a pale yellow oily substance (1.8 g, 73%).
1H NMR (CDCl3, 400 MHz): δ 1.36 (m, 2H), 1.56-1.69 (m, 4H), 1.90 (m, 2H), 2.30 (t, J=7.48, 2H), 2.73 (t, J=5.97, 2H), 3.27-3.35 (m, 4H), 3.63 (s, 3H), 6.51 (d, J=8.97, 1H), 7.41 (s, 1H), 7.49 (dd, J=1.92, 8.60 Hz, 1H), 9.60 (s, 1H); LRMS (ESI): m/z calcd for C17H24NO3+ [M+H]+: 290.2, found: 290.1.
To methanol (10 mL) in which 6-formyl-1,2,3,4-tetrahydroquinoline-methyl hexanoate (1.08 g) had been dissolved was added 5 N potassium hydroxide (6 mL). Next, the mixture was stirred at room temperature for 5 hours. After the solvent was removed under pressure from the reaction mixture, the remainder was diluted with water and washed with ethyl acetate. An aqueous hydrochloric acid solution was added to an aqueous layer to bring the pH to 1. Subsequently, the mixture was extracted using ethyl acetate and the extract was dried over anhydrous sodium sulfate. After the organic solvent was removed under reduced pressure, the remainder was purified by silica gel column chromatography (using an eluent ratio of dichloromethane:methanol=10:1; 1% AcOH was included) to give 6-formyl-1,2,3,4-tetrahydroquinoline-hexanoic acid as a brown solid (914 mg, 89%).
1H NMR (CDCl3, 400 MHz): δ 1.39 (m, 2H), 1.59-1.70 (m, 4H), 1.92 (m, 2H), 2.37 (t, J=7.41 Hz, 2H), 2.75 (t, J=6.29, 2H), 3.29-3.37 (m, 4H), 6.53 (d, J=8.54, 1H), 7.43 (s, 1H), 7.51 (d, J=8.50, 1H), 9.62 (s, 1H); LRMS (ESI): m/z calcd for C16H22NO3+ [M+H]+: 276.2, found: 276.1.
(1) To an acetonitrile suspension (100 mL) containing sodium iodide (31.7 g, 191 mmol) and sodium carbonate (22 g, 160 mmol) were added 1,2,3,4-tetrahydroquinoline (10 mL, 79.6 mmol) and methyl bromoacetate (8.8 mL, 95.5 mmol). Next, the reaction solution was heated under reflux for 6 hours. Next, the reaction solution was cooled to room temperature. Subsequently, the reaction mixture was filtered and the organic solvent was removed under reduced pressure. The remainder was diluted with water and then extracted using ethyl acetate. The organic layer was washed with saturated saline and dried over anhydrous sodium sulfate. Finally, the organic solvent was removed under reduced pressure. Additional purification work was not conducted and the resultant was used in the next reaction.
1H NMR (CDCl3, 500 MHz): δ 1.98 (m, 2H), 2.77 (t, J=6.27 Hz, 2H), 3.38 (t, J=5.72 Hz, 2H), 3.70 (s, 3H), 3.99 (s, 2H), 6.38 (d, J=7.45 Hz, 1H), 6.60 (dd, J=7.45, 7.45 Hz, 1H), 6.95 (d, J=7.45 Hz, 1H), 7.00 (dd, J=7.45, 7.45 Hz, 1H).
(2) Lithium aluminum hydride (3.62 g, 95.5 mmol) was dissolved in tetrahydrofuran (100 mL) and the mixture was cooled to 0° C. To this solution was added over 30 min tetrahydrofuran (400 mL) in which 1,2,3,4-tetrahydroquinoline-methyl ethanoate obtained in the above section (1) had been dissolved. Next, the mixture was stirred at 0° C. for 20 min. Subsequently, the temperature of the mixture was raised to room temperature and the mixture was then stirred for 12 hours. Then, the reaction mixture was cooled to 0° C. Thereafter, water (3.6 mL), 15% aqueous sodium hydroxide (3.6 mL), and water (10.8 mL) were added thereto in this order, and the resulting mixture was further stirred at room temperature for 1 hour. After that, precipitates were removed by celite filtration. After the organic solvent was removed under reduced pressure, the remainder was purified by silica gel column chromatography (using an eluent ratio of hexane:ethyl acetate=1:1) to give a yellow oily substance (12.1 g, 85% over 2 step).
1H NMR (CDCl3, 500 MHz): δ 1.82 (br, 1H), 1.95 (m, 2H), 2.76 (t, J=6.30 Hz, 2H), 3.31 (t, J=5.72 Hz, 2H), 3.42 (t, J=5.72 Hz, 2H), 3.80 (br, 2H), 6.60 (dd, J=6.85, 7.45 Hz, 1H), 6.67 (d, J=8.00 Hz, 1H), 6.95 (d, J=6.85 Hz, 1H), 7.04 (dd, J=7.45, 8.00 Hz, 1H)
(3) To dichloromethane (40 mL) in which 1,2,3,4-tetrahydroquinoline-1-ethanol had been dissolved was added trimethyl amine (1.74 mL, 12.48 mmol). Next, the mixture was stirred at 0° C. for 10 min. Then, methanesulfonyl chloride (725 μL, 9.36 mmol) at 0° C. was added thereto. After the temperature of the reaction mixture was raised to room temperature, the reaction mixture was stirred for 3 hours. Subsequently, the reaction mixture was concentrated under reduced pressure. Then, the concentrate was dissolved in a small amount of ethyl acetate and was subjected to silica gel filtration. The ethyl acetate was removed under reduced pressure to give a yellow oily substance. This oily substance and sodium azide (1.62 g, 25 mmol) were dissolved in DMF (40 mL) and the mixture was then stirred at 60° C. for 1 hour. After that, the reaction solution was diluted with water and was extracted using diethyl ether. The organic layer was washed with saturated saline and dried over anhydrous sodium sulfate. After the organic solvent was removed under reduced pressure, the remainder was purified by silica gel column chromatography (using an eluent ratio of hexane:ethyl acetate=25:1) to give a substance of interest as a colorless oily substance (1128 mg, 89%).
1H NMR (CDCl3, 500 MHz): δ 1.97 (m, 2H), 2.77 (t, J=6.27 Hz, 2H), 3.36 (t, J=5.73 Hz, 2H), 6.58 (d, J=8.00 Hz, 1H), 6.62 (dd, J=7.43, 7.45, 1H), 6.97 (d, J=7.43 Hz), 7.07 (dd, J=7.45, 8.00 Hz, 1H)
(4) To DMF (1.5 mL, 18.4 mmol) was added phosphoryl chloride (572 μL, 6.14 mmol) at 0° C. Next, the temperature of the reaction mixture was raised to room temperature and the reaction mixture was stirred for 1 hour. Then, 1-(2-azidoethyl)-1,2,3,4-tetrahydroquinoline (1128 mg, 5.58 mmol) was added thereto and the reaction was further carried out at 90° C. for 5 hours. After that, the reaction mixture was cooled to 0° C. and diluted with water. Subsequently, a potassium carbonate aqueous solution was added to bring the pH to 7, and the mixture was extracted using diethyl ether. The organic layer was washed with saturated saline and dried over anhydrous sodium sulfate. After the organic solvent was removed under reduced pressure, the remainder was purified by silica gel column chromatography (using an eluent ratio of hexane:ethyl acetate=3:1) to give a substance of interest as a pale yellow oily substance.
1H NMR (CDCl3, 400 MHz): δ 1.95 (m, 2H), 2.77 (t, J=6.28 Hz, 2H), 3.43 (t, J=5.84 Hz, 2H), 3.48-3.56 (m, 4H), 6.58 (d, J=8.63 Hz, 1H), 7.45 (d, J=2.23 Hz, 1H), 7.52 (dd, J=2.23, 8.63 Hz, 1H), 9.65 (s, 1H); LRMS (ESI): m/z calcd for C12H15N4O+ [M+H]+: 231.1, found: 231.3.
Except that compound 6 and 1-(2-azidoethyl)-6-formyl-1,2,3,4-tetrahydroquinoline were used, the same synthesis procedure as in Synthesis Example 8 was repeated to give compound 17 (the yield: 29%).
1H NMR (acetone-d6, 500 MHz): δ 1.97 (m, 2H), 2.25 (s, 3H), 2.35 (s, 3H), 2.56 (s, 3H), 3.47-3.66 (m, 8H), 6.78 (d, J=8.57 Hz, 1H), 7.05 (s, 1H), 7.26 (s, 1H), 7.34 (d, J=8.57 Hz, 1H), 7.40 (d, J=16.1 Hz, 1H), 7.53 (s, 1H), 7.56 (d, J=16.1 Hz, 1H)
(1) Target compound 18 was synthesized using Fmoc-protected amino acids and 4-pentynoic acid in accordance with a conventional Fmoc-type peptide solid phase synthesis method.
LRMS (ESI): m/z calcd for C46H61N4O7+ [M+H]+: 809.5, found: 809.5; Purity >80% (based on HPLC analysis at 214 nm with a linear gradient of 0-100% MeCN in 0.1% aq. TFA over 40 min).
(2)
According to the disclosure of K. Shinoa, Y. Sohma, M. Kanai, Bioorg. Med. Chem. Lett. 2015, 25, 2976-2979, compound 17 (9.42 mg, 0.0124 mmol) and compound 18 (15 mg, 0.0186 mmol) were dissolved in 1.5 mL of toluene/tBuOH/water (1/1/1). Next, CuSO4.5 H2O (0.31 mg, 10 mol %) and L-ascorbic acid (11 mg, 0.062 mmol) were added thereto, and the mixture was then stirred for 24 hours. After that, the reaction mixture was purified by HPLC to give compound 19 (2.0 mg, 11%).
MALDI-TOF MS: m/z calcd for C72H87BF4IN12O7+ [M+H]30: 1445.6, found: 1448.5.
Phosphate buffer solutions (pH 7.4) in which compound 8, 10, 11, or 12 (each 20 μM) had been dissolved were incubated at 37° C. and the time course of each light absorption spectrum was determined.
The results are shown in
The optical properties (in methanol) of compounds 2 to 4 synthesized were determined (Table 1). The compounds 2 to 4 each have maximum absorption at a wavelength at or near 500 nm and emit fluorescence at 509 nm. It was observed that introduction of a perfluoroalkyl group caused some decreases in light absorption coefficients, but little change in their fluorescence quantum yield. This result has demonstrated that the perfluoroalkyl group on the boron atom does not considerably affect the optical properties of the present compounds.
Next, a methanol solution containing each of compounds 2 to 4 was irradiated with light of 500 nm, and incubated at 37° C. Then, a change in the time course of the absorbance at the maximum light absorption wavelength was measured to compare their photostability (
Each absorption spectrum was measured (Table 2). The maximum absorption wavelength of compound 8, in which the dimethylamino phenyl group of compound 9 was changed to a julolidine group, was shifted to the long-wavelength side by 30 nm than that of compound 9. Even when the substituent on the boron atom was changed from F to CF3 (compound 11), there was almost no observable change in the maximum absorption wavelength. The maximum absorption wavelength of compound 12, in which R6 was iodine, was shifted to the long-wavelength side by 20 nm. This allowed the compound to be efficiently excited by light with a longer wavelength of over 650 nm. In the cases of the carboxyl group- or sulfonic acid group-substituted tetrahydroquinoline derivatives (compounds 13 to 16) so as to introduce a water-soluble substituent, the absorption wavelength was shifted to the short-wavelength side by about 10 nm than that of the julolidine-substituted derivative. It can be deduced from the above results that as the lone electron pair of the nitrogen atom is arranged more stably on the same plane as that of the benzene ring due to the condensed ring structure of the julolidine or tetrahydroquinoline, the absorption wavelength tends to be shifted more to the long-wavelength side. Also, it was found that introduction of iodine caused a shift to the long-wavelength side and that the substituent on the boron atom did not significantly affect the absorption wavelength.
(1) To check whether or not the compounds synthesized actually had Aβ oxygenation activity, in vitro oxygenation experiments were carried out using Aβ1-42 as a substrate. Compound 12 (20 μM) was added to a phosphate buffer solution (pH 7.4) containing Aβ1-42 (20 μM), and the mixture was incubated at 37° C. under LED light irradiation (at a wavelength of 595 nm). Then, a mass spectrometer (MALDI-TOF MS) was used to follow the reaction. Before the light irradiation, native Aβ1-42 and Na+ adducts were primarily observed. After the light irradiation, however, there appeared ion peaks indicating the presence of oxygen adducts in a time-dependent manner (
(2) The Aβ1-42 oxygenation activities of compounds 8, 11, and 12 were compared (
(3) Next, the activities of compounds 12, 14, and 16 were compared (
(4) To obtain more direct knowledge, compounds 13 to 15 were used to further investigate the functions of an oxygenation photocatalyst having a perfluoroalkyl group on the boron atom. First, the Aβ1-42 oxygenation activities of the compounds 13 to 15 were likewise compared (
Compound 14 was used to investigate whether or not a compound of the present invention was able to selectively oxygenate Aβ. First, compound 14 (20 μM) was added to phosphate buffer solutions (pH 7.4) each containing Aβ1-42 (20 μM), angiotensin IV (20 μM) or a methionine enkephalin (20 μM), which solutions had been pre-incubated for 3 hours. The mixtures were incubated at 37° C. for 30 min under LED light irradiation (at a wavelength of 595 nm). Then, a mass spectrometer (MALDI-TOF MS) was used to follow the reaction. Three model peptides having a residue capable of being oxygenated were selected, including angiotensin IV (AT-4), met-enkephalin (ME), and somatostatin (Sat). These three peptides were subjected to the same oxygenation conditions as for Aβ1-42 (
In the following, in order to check whether or not compound 14 recognized a cross-β-sheet structure characteristic of Aβ aggregates to elicit oxygenation activity, dependency of the oxygenation rate on aggregation was examined. An incubation time after o-acyliso-Aβ1-42 was converted to native Aβ1-42 was used as an index for aggregation process index to check whether or not the oxygenation rate changed depending on the incubation time (
(Method)
Aβ (40 μM) was dissolved in a phosphate buffer solution, and the mixture was incubated at 37° C. for 2 hours. Next, compound 16 was added thereto (at 1.6 μM). Then, 0.1% horse serum-containing Dulbecco's Modified Eagle Medium was added to rat adrenal pheochromocytoma PC12 cells (purchased from RIKEN, Japan) seeded on a poly D-lysine-coated 96-well plate to prepare a mixture. To the mixture was added 25 μL of the above phosphate buffer solution containing Aβ and compound 16 (the final volume: 100 μL; the final Aβ concentration: 10 μM; the final compound 16 concentration: 0.4 μM). After that, this mixed solution was incubated at 37° C. for 5 min under LED light irradiation (at a wavelength of 660 nm; at 10 mW) (provided that in the dark, no light irradiation). The plate containing the cell reaction solution was further incubated at 37° C. for 48 hours under 5% CO2 atmosphere. Finally, a viable-cell count reagent SF containing WST-8 (10 μL; purchased from Nacalai Tesque, Inc.) was added and the resulting mixture was incubated at 37° C. for 3 hours under 5% CO2 atmosphere to measure absorbance at 450 nm (cf., a reference wavelength: 655 nm). From the absorbance, cell viability was determined.
(Results)
Whether or not the oxygenation of Aβ1-42 in the presence of cells caused a decrease in cytotoxicity was investigated. Rat adrenal pheochromocytoma model nerve cells PC12 were used as cells and compound 16 with excellent water solubility and oxygenation activity was used as a catalyst to perform a cell viability (
The present compound represented by formula (1A) has a structure in which an electron donor site and an electron acceptor site rotate around a bond therebetween as a shaft. Even when excited by light in the absence of Aβ, the present compound causes no reaction because of the rotation and resultant relaxation. By contrast, when the compound binds to the higher structure of amyloid and the rotation is inhibited, an oxygenation reaction occurs through the production of singlet oxygen. It is considered that since the activity of the present compound is switchable as describe above, the present compound seems can oxygenate Aβ1-42 in a highly selectable manner even in a complicated system, for example in the presence of cells.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2015-044839 | Mar 2015 | JP | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/JP2016/056803 | 3/4/2016 | WO | 00 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2016/143699 | 9/15/2016 | WO | A |
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| 20110287473 | De Barry et al. | Nov 2011 | A1 |
| 20150158841 | Ran et al. | Jun 2015 | A1 |
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| 2009-280567 | Dec 2009 | JP |
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| WO 2010125907 | Nov 2010 | WO |
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| Number | Date | Country | |
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| 20180042948 A1 | Feb 2018 | US |
