Patent Application
20240239816
The present invention relates to borono-phenylalanine derivatives.
One of the methods for treating cancer is boron neutron capture therapy (BNCT). Boron neutron capture therapy is a therapeutic method in which a boron compound containing a boron-10 isotope (10B) is taken up into cancer cells and irradiated with low-energy neutron rays (for example, thermal neutrons) to locally destroy the cancer cells by a nuclear reaction occurring in the cells. In this therapeutic method, since it is important to selectively accumulate a boron compound containing 10B in cells of cancer tissue in order to enhance the therapeutic effect, it is necessary to develop a boron compound that is selectively taken up by cancer cells.
As an agent used for BNCT, a 4-borono-phenylalanine derivative in which a boron atom or a boron atom group is introduced into a basic skeleton has been synthesized. As an agent in use in actual clinical practice, there are a 4-borono-phenylalanine derivative (L-BPA) and mercaptoundecahydrododecaborate (BSH). 4-Borono-phenylalanine is taken up by L-type amino acid transporter 1 (LAT1), which is a type of amino acid transporter, as a mimic of phenylalanine. Since expression of LAT1 is enhanced in cancer cells, L-BPA is likely to accumulate and is used for treatment of cancer by utilizing its properties (see, for example, Patent Document 1).
There is a need for further development of new compounds having the property of being specifically taken up into cancer cells.
An object of the present invention is to provide borono-phenylalanine derivatives.
As a result of intensive studies to solve the above problems, the present inventors have found new derivatives of borono-phenylalanine and have accomplished the present invention.
That is, the present invention provides the following compound.
[1]
A compound represented by the following formula (I) or a pharmaceutically acceptable salt thereof:
The compound or a pharmaceutically acceptable salt thereof according to [1], wherein R6 represents methyl or ethyl.
[3]
The compound or a pharmaceutically acceptable salt thereof according to [1] or [2], wherein R7 represents boronic acid (B(OH)2) or a pinacol ester of boronic acid.
[4]
The compound or a pharmaceutically acceptable salt thereof according to any one of [1] to [3], wherein R5 represents H.
[5]
The compound or a pharmaceutically acceptable salt thereof according to any one of [1] to [4], wherein any one or two or more of R1, R2, R3, and R4 are each independently Cl, F, C1-4 alkyl, C1-4 alkoxy, C1-4 alkylthio, CH2X, CHX2, or CX3 (X represents F).
[6]
An L-type amino acid transporter 1 (LAT1)-selective agent including the compound or a pharmaceutically acceptable salt thereof according to any one of [1] to [5].
[7]
An agent for BNCT, including the compound or a pharmaceutically acceptable salt thereof according to any one of [1] to [5].
[8]
A diagnostic agent containing a radioisotope, the diagnostic agent including the compound or a pharmaceutically acceptable salt thereof according to any one of [1] to [5].
[9]
A compound represented by the following formula (III) or a pharmaceutically acceptable salt thereof:
A compound represented by the following formula (IV) or a pharmaceutically acceptable salt thereof:
is excluded.
The novel compounds of the present invention are superior in uptake into LAT1 expressing cells and can be conveniently used for boron neutron capture therapy.
In the present specification, when a compound having an asymmetric carbon is represented, the compound may be any of a racemate, an R-isomer, and an S-isomer unless otherwise indicated.
In one aspect of the present invention, the borono-phenylalanine derivative is a 4-borono-phenylalanine derivative, and is a compound represented by the following formula (I) or a pharmaceutically acceptable salt thereof.
In the present specification, the halogen may be any of F, Cl, Br, and I, and is particularly preferably F, Cl, or Br.
In the present specification, the C1-C6 alkyl refers to a linear or branched C1-C6 saturated hydrocarbon group. This definition also includes those used in alkoxy, alkoxyalkyl, and haloalkyl. Examples of the C1-6 alkyl group include methyl, ethyl, propyl (e.g., n-propyl and isopropyl), butyl (e.g., n-butyl, isobutyl, and t-butyl), and pentyl (e.g., n-pentyl, isopentyl, and neopentyl). The C1-6 alkyl group is preferably a linear or branched C1-C4 alkyl group, and examples thereof include, but are not limited to, a methyl group, an ethyl group, an isopropyl group, and a butyl group.
In the present specification, C1-C6 haloalkyl refers to a C1-C6 alkyl group having one or more halogen substituents. The haloalkyl group is preferably represented by C2X5, CH2X, CHX2, or CX3 (X represents Cl, F, Br, or I), and examples thereof include, but are not limited to, CF3, C2F5, CHF2, CCl3, CHCl2, and C2Cl5.
In the present specification, C1-C6 alkoxy represents a group having a linear or branched C1-C6 alkyl group and an oxygen atom. C1-C6 alkoxy preferably has a linear or branched C1-C4 alkyl, and examples thereof include, but are not limited to, methoxy, ethoxy, isopropoxy, and butoxy.
In the present specification, examples of C1-6 alkoxy C1-6 alkyl include, but are not limited to, methoxyethyl and ethoxyethyl.
In the present specification, examples of C1-C6 alkylaminocarbonyl (CONR8R9 (R8 and R9 each independently represent H or C1-6 alkyl)) include, but are not limited to, methylaminocarbonyl, dimethylaminocarbonyl, ethylaminocarbonyl, and i-propylaminocarbonyl.
In the present specification, examples of C1-C6 alkylcarbonyl include, but are not limited to, methylcarbonyl and ethylcarbonyl.
In the present specification, examples of C1-C6 alkoxycarbonyl include, but are not limited to, methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl, isobutoxycarbonyl, sec-butoxycarbonyl, tert-butoxycarbonyl, pentyloxycarbonyl, and hexyloxycarbonyl.
In the present specification, examples of COOR10 (R10 is H or C1-6 alkyl, amino, alkylamino (NR11 R12 (R11 and R12 each independently represent H or C1-6 alkyl)) include, but are not limited to, methyloxycarbonyl and aminooxycarbonyl.
In the present specification, examples of haloalkylsulfanyl, haloalkylsulfinyl, and haloalkylsulfonyl include, but are not limited to, trifluoromethylsulfanyl, a trifluoromethylsulfinyl group, and trifluoromethylsulfonyl.
In the present specification, examples of C1-C6 alkylthio include, but are not limited to, methylthio, ethylthio, propylthio, isopropylthio, butylthio, sec-butylthio, tert-butylthio, pentylthio, and hexylthio.
In the present specification, examples of C1-C6 alkylsulfinyl include, but are not limited to, methylsulfinyl and ethylsulfinyl.
In the present specification, examples of C1-C6 alkylsulfonyl include, but are not limited to, methylsulfonyl and ethylsulfonyl.
In the derivatives of the present invention, R7 represents any of a boronic acid group (—B(OH)2), a boronic acid ester group, or a boronic acid amide group: for example, the boronic acid ester group and the boronic acid amide group in this definition represent a group having a chain structure such as —B(NR41)2 or —B(OR41)2 at the position of R7 or a group having a cyclic structure together with an atom B at the position of R7. Here, R41 represents a linear or branched C1-C10 alkyl group. Here, the “linear or branched C1-C10 alkyl group” may be any alkyl group having 1-10 carbon atoms. The alkyl group is preferably a linear or branched C1-C8 alkyl group, and more preferably a linear or branched C1-C6 alkyl group. Examples of these groups include, but are not limited to, a methyl group, an ethyl group, an isopropyl group, and a butyl group. Furthermore, in the cyclic structure referred to herein, the atom that is interposed is not necessarily only an O atom, and may be an N atom. Examples thereof include, but are not limited to, groups composed of an ester or ester analog composed of an atom B and any selected from the group consisting of pinacol, 2,2-dimethyl-1,3-propanediol, N-methyldiethanolamine, 1-6-diaminonaphthalene, N-methyliminodiacetic acid, 1,1,1-trishydroxymethylethane, and catechol. Examples thereof include, but are not limited to, a boronic acid pinacol ester, a boronic acid MIDA ester, a boronic acid 1,3-propanediol ester, a boronic acid neopentyl glycol ester, a boronic acid catechol ester, a boronic acid pinanediol ester, a boronic acid biscyclohexyldiol ester, a boronic acid MPM ester, a trifluoroborate salt, a cyclic triol borate salt, and a cyclic compound formed of diaminonaphthalenamide and boron.
Among them, R7 is particularly preferably boronic acid or a boronic acid ester having a chain or cyclic structure, and most preferably boronic acid.
Here, as to boron atoms, the proportion of boron-10 is not limited, but may be preferably 75% by mass or more, more preferably 80% by mass or more, even more preferably 90% by mass or more, and particularly preferably 95% by mass or more.
Natural boron (boron) includes boron-10 and boron-11 as isotopes in the proportions of 20% boron-10 to 80% boron-11. Therefore, prior to the production of the 4-borono-phenylalanine derivative of the present invention, it is also preferable to concentrate boron having a mass number of 10 (boron-10). In the present invention, for example, a commercially available product may be used as the boron atom source. As such a commercially available product, for example, 10B concentrated boric acid (manufactured by Stella Chemifa Corporation) can be used.
Here, boron-10 can be measured by multi-type ICP-optical emission spectrometry (ICP-OES) using Agilent 710 (manufactured by Agilent Technologies Inc.). The ICP-OES used for the measurement is adjusted in accordance with JIS K 0116.
In the compound, it is particularly preferable that any one or two or more of R1, R2, R3, and R4 are each independently, but are not limited to, Cl, F, C1-3 alkyl, C1-3 alkoxy, CH2F, CHF2, CF3, or C1-3 alkylthio.
In the compound, R5 particularly preferably represents, but is not limited to, H. At this time, however, it is impermissible that all of R1, R2, R3, and R4 are H.
In the compound, R6 particularly preferably represents, but is not limited to, methyl or ethyl.
In the compound, R7 is particularly preferably, but is not limited to, boronic acid (B(OH)2) or a pinacol ester of boronic acid.
In one aspect of the present invention, it is preferable that in the compound, any one or two or more of R1, R2, R3, and R4 are each independently Cl or F, R5 represents H, R6 represents methyl, and R7 represents boronic acid (B(OH)2).
In another aspect of the present invention, it is preferable that in the compound, any one or two or more of R1, R2, R3, and R4 are each independently CH2F, CHF2, or CF3, R5 represents H, R6 represents methyl, and R7 represents boronic acid (B(OH)2).
In still another aspect of the present invention, it is preferable that in the compound, any one or two or more of R1, R2, R3, and R4 are each independently methyl or ethyl, R5 represents H, R6 represents methyl, and R7 represents boronic acid (B(OH)2).
In still another aspect of the present invention, it is preferable that in the compound, any one or two or more of R1, R2, R3, and R4 are each independently methoxy or ethoxy, R5 represents H, R6 represents methyl, and R7 represents boronic acid (B(OH)2).
In still another aspect of the present invention, it is preferable that in the compound, any one or two or more of R1, R2, R3, and R4 are each independently methylthio or ethylthio, R5 represents H, R6 represents methyl, and R7 represents boronic acid (B(OH)2).
In still another aspect of the present invention, it is preferable that in the compound, all of R1, R2, R3, and R4 are H, R5 represents H, R6 represents ethyl, and R7 represents boronic acid (B(OH)2).
Examples of the “pharmaceutically acceptable salt” as used in the present invention includes a salt with an inorganic base, a salt with an organic base, a salt with an inorganic acid, a salt with an organic acid, and a salt with a basic or acidic amino acid. Suitable examples of the salt with an inorganic base include alkali metal salts such as sodium salts and potassium salts; alkaline earth metal salts such as calcium salts and magnesium salts; aluminum salts and ammonium salts. Suitable examples of the salt with an organic base include salts with trimethylamine, triethylamine, pyridine, picoline, ethanolamine, diethanolamine, triethanolamine, dicyclohexylamine, and N,N′-dibenzylethylenediamine. Suitable examples of the salt with an inorganic acid include salts with hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, and phosphoric acid. Suitable examples of the salt with an organic acid include salts with formic acid, acetic acid, trifluoroacetic acid, fumaric acid, oxalic acid, tartaric acid, maleic acid, citric acid, succinic acid, malic acid, methanesulfonic acid, benzenesulfonic acid, and p-toluenesulfonic acid. Suitable examples of the salt with a basic amino acid include salts with arginine, lysine, and ornithine, and suitable examples of the salt with an acidic amino acid include salts with aspartic acid and glutamic acid.
Another aspect of the present invention relates to a compound represented by the following formula (III) or a pharmaceutically acceptable salt thereof.
Here, the conditions including the type of substituent, suitable examples, the type of salt, and the like are as described in the section of [4-Borono-phenylalanine derivatives].
Yet another aspect of the present invention relates to a compound represented by the following formula (IV) or a pharmaceutically acceptable salt thereof.
is excluded.
Here, among such compounds, a compound represented by the following chemical formula may be particularly preferable.
is excluded.
In the present specification, the halogen may be any of F, Cl, Br, and I, and is particularly preferably F, Cl, or Br.
In the present specification, the C1-C6 alkyl refers to a linear or branched C1-C6 saturated hydrocarbon group. This definition also includes those used in alkoxy, alkoxyalkyl, and haloalkyl. Examples of the C1-6 alkyl group include methyl, ethyl, propyl (e.g., n-propyl and isopropyl), butyl (e.g., n-butyl, isobutyl, and t-butyl), and pentyl (e.g., n-pentyl, isopentyl, and neopentyl). The C1-6 alkyl group is preferably a linear or branched C1-C4 alkyl group, and examples thereof include, but are not limited to, a methyl group, an ethyl group, an isopropyl group, and a butyl group.
In the present specification, C1-C6 haloalkyl refers to a C1-C6 alkyl group having one or more halogen substituents. The haloalkyl group is preferably represented by C2X5, CH2X, CHX2, or CX3 (X represents Cl, F, Br, or I), and examples thereof include, but are not limited to, CF3, C2F5, CHF2, CCl3, CHCl2, and C2Cl5.
In the present specification, C1-C6 alkoxy represents a group having a linear or branched C1-C6 alkyl group and an oxygen atom. C1-C6 alkoxy preferably has a linear or branched C1-C4 alkyl, and examples thereof include, but are not limited to, methoxy, ethoxy, isopropoxy, and butoxy.
In the present specification, examples of C1-6 alkoxy C1-6 alkyl include, but are not limited to, methoxyethyl and ethoxyethyl.
In the present specification, examples of C1-C6 alkylaminocarbonyl (CONR8R9 (R8 and R9 each independently represent H or C1-6 alkyl)) include, but are not limited to, methylaminocarbonyl, dimethylaminocarbonyl, ethylaminocarbonyl, and i-propylaminocarbonyl.
In the present specification, examples of C1-C6 alkylcarbonyl include, but are not limited to, methylcarbonyl and ethylcarbonyl.
In the present specification, examples of C1-C6 alkoxycarbonyl include, but are not limited to, methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl, isobutoxycarbonyl, sec-butoxycarbonyl, tert-butoxycarbonyl, pentyloxycarbonyl, and hexyloxycarbonyl.
In the present specification, examples of COOR10 (R10 is H or C1-6 alkyl, amino, alkylamino (NR11 R12 (R11 and R12 each independently represent H or C1-6 alkyl)) include, but are not limited to, methyloxycarbonyl and aminooxycarbonyl.
In the present specification, examples of haloalkylsulfanyl, haloalkylsulfinyl, and haloalkylsulfonyl include, but are not limited to, trifluoromethylsulfanyl, a trifluoromethylsulfinyl group, and trifluoromethylsulfonyl, respectively.
In the present specification, examples of C1-C6 alkylthio include, but are not limited to, methylthio, ethylthio, propylthio, isopropylthio, butylthio, sec-butylthio, tert-butylthio, pentylthio, and hexylthio.
In the present specification, examples of C1-C6 alkylsulfinyl include, but are not limited to, methylsulfinyl and ethylsulfinyl.
In the present specification, examples of C1-C6 alkylsulfonyl include, but are not limited to, methylsulfonyl and ethylsulfonyl.
In the present specification, examples of the unsubstituted C5-7 cycloalkyl include cyclopentyl, cyclohexyl, and cycloheptyl.
In the present specification, examples of the unsubstituted C6-7 cycloalkenyl include cyclohexenyl and cycloheptenyl.
In the derivatives of the present invention, R7 represents any of a boronic acid group (—B(OH)2), a boronic acid ester group, or a boronic acid amide group: for example, the boronic acid ester group and the boronic acid amide group in this definition represent a group having a chain structure such as —B(NR41)2 or —B(OR41)2 at the position of R7 or a group having a cyclic structure together with an atom B at the position of R7. Here, R41 represents a linear or branched C1-C10 alkyl group. Here, the “linear or branched C1-C10 alkyl group” may be any alkyl group having 1-10 carbon atoms. The alkyl group is preferably a linear or branched C1-C8 alkyl group, and more preferably a linear or branched C1-C6 alkyl group. Examples of these groups include, but are not limited to, a methyl group, an ethyl group, an isopropyl group, and a butyl group. Furthermore, in the cyclic structure referred to herein, the atom that is interposed is not necessarily only an O atom, and may be an N atom. Examples thereof include, but are not limited to, groups composed of an ester or ester analog composed of an atom B and any selected from the group consisting of pinacol, 2,2-dimethyl-1,3-propanediol, N-methyldiethanolamine, 1-6-diaminonaphthalene, N-methyliminodiacetic acid, 1,1,1-trishydroxymethylethane, and catechol. Examples thereof include, but are not limited to, a boronic acid pinacol ester, a boronic acid MIDA ester, a boronic acid 1,3-propanediol ester, a boronic acid neopentyl glycol ester, a boronic acid catechol ester, a boronic acid pinanediol ester, a boronic acid biscyclohexyldiol ester, a boronic acid MPM ester, a trifluoroborate salt, a cyclic triol borate salt, and a cyclic compound formed of diaminonaphthalenamide and boron.
Among them, R7 is particularly preferably boronic acid or a boronic acid ester having a chain or cyclic structure, and most preferably boronic acid.
Here, as to boron atoms, the proportion of boron-10 is not limited, but may be preferably 75% by mass or more, more preferably 80% by mass or more, even more preferably 90% by mass or more, and particularly preferably 95% by mass or more.
Natural boron (boron) includes boron-10 and boron-11 as isotopes in the proportions of 20% boron-10 to 80% boron-11. Therefore, prior to the production of the 4-borono-phenylalanine derivative of the present invention, it is also preferable to concentrate boron having a mass number of 10 (boron-10). In the present invention, for example, a commercially available product may be used as the boron atom source. As such a commercially available product, for example, 10B concentrated boric acid (manufactured by Stella Chemifa Corporation) can be used.
Here, boron-10 can be measured by multi-type ICP-optical emission spectrometry (ICP-OES) using Agilent 710 (manufactured by Agilent Technologies Inc.). The ICP-OES used for the measurement is adjusted in accordance with JIS K 0116.
It is particularly preferable that in the compound, any one to three of R20 are each independently, but are not limited to, H, Cl, F, C1-3 alkyl, C1-3 alkoxy, CH2F, CHF2, CF3, or C1-3 alkylthio.
In the compound, R7 is particularly preferably, but is not limited to, boronic acid (B(OH)2) or a pinacol ester of boronic acid.
In one aspect of the present invention, it is preferable that in the compound, any one to three of R20 are each independently Cl or F, x is 1 to 3, and R7 represents boronic acid (B(OH)2).
In another aspect of the present invention, it is preferable that in the compound, any one to three of R20 are each independently CH2F, CHF2, or CF3, x is 1 to 2, and R7 represents boronic acid (B(OH)2).
In still another aspect of the present invention, it is preferable that in the compound, any one to three of R20 are each independently methyl or ethyl, x is 1 to 2, and R7 represents boronic acid (B(OH)2).
In still another aspect of the present invention, it is preferable that in the compound, any one or two or more of R1, R2, R3, and R4 are each independently methoxy or ethoxy, R5 represents H, R6 represents methyl, and R7 represents boronic acid (B(OH)2).
In still another aspect of the present invention, it is preferable that in the compound, any one to three of R20 are each independently methylthio or ethylthio, x is 1 to 3, and R7 represents boronic acid (B(OH)2).
Examples of the “pharmaceutically acceptable salt” as used in the present invention includes a salt with an inorganic base, a salt with an organic base, a salt with an inorganic acid, a salt with an organic acid, and a salt with a basic or acidic amino acid. Suitable examples of the salt with an inorganic base include alkali metal salts such as sodium salts and potassium salts; alkaline earth metal salts such as calcium salts and magnesium salts; aluminum salts and ammonium salts. Suitable examples of the salt with an organic base include salts with trimethylamine, triethylamine, pyridine, picoline, ethanolamine, diethanolamine, triethanolamine, dicyclohexylamine, and N,N′-dibenzylethylenediamine. Suitable examples of the salt with an inorganic acid include salts with hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, and phosphoric acid. Suitable examples of the salt with an organic acid include salts with formic acid, acetic acid, trifluoroacetic acid, fumaric acid, oxalic acid, tartaric acid, maleic acid, citric acid, succinic acid, malic acid, methanesulfonic acid, benzenesulfonic acid, and p-toluenesulfonic acid. Suitable examples of the salt with a basic amino acid include salts with arginine, lysine, and ornithine, and suitable examples of the salt with an acidic amino acid include salts with aspartic acid and glutamic acid.
Among the borono-phenylalanine derivatives of the present invention, one selected from the group consisting of the following compounds or a salt thereof is particularly preferable.
In the present invention, the method for producing a novel 4-borono-phenylalanine derivative is not limited, and an ordinary amino acid synthesis method is used. A particularly preferable method may be, but is not limited to, the following method, for example.
First, a protected amino acid is reacted with an organic halide represented by the following general formula (II) in the presence of an organic solvent, a basic aqueous solution, and a phase transfer catalyst.
In the formula (II),
Here, as the organic halide represented by the formula (II), a compound prepared by a known method can be used as it is, or a commercially available product can be used.
Among them, for example, 4-iodobenzyl bromide and 4-bromo-2-fluorobenzyl bromide are available from Tokyo Chemical Industry Co., Ltd. or Combi-Blocks Inc. 4-Bromo-3-fluorobenzyl bromide, 1-bromo-4-(bromomethyl)-2,3-difluorobenzene, 4-bromo-1-(bromomethyl)-2-methoxybenzene, 4-bromo-1-(bromomethyl)-2-nitrobenzene, 4-bromo-1-(bromomethyl)-2-chlorobenzene, 4-bromo-1-(bromomethyl)-2-(trifluoromethyl)benzene, 4-bromo-1-(bromomethyl)-2-(trifluoromethyl)benzene, 1-bromo-4-(bromomethyl)-2-methylbenzene are available from Combi-Blocks Inc. 4-Bromo-1-(bromomethyl)-2-methylbenzene and 4-bromo-2,6-difluorobenzyl bromide are available from Fluorochem Ltd.
5-Bromo-2-(bromomethyl)benzonitrile can be prepared, for example, from 5-bromo-2-methylbenzonitrile available from Tokyo Chemical Industry Co., Ltd., 1-bromo-4-(bromomethyl)-2-chloro-5-fluorobenzene can be prepared from 4-bromo-5-chloro-2-fluorotoluene available from Combi-Blocks Inc., and 4-bromo-1-(bromomethyl)-2-(methylthio)benzene can be prepared from 5-bromo-2-methylthioanisole available from Combi-Blocks Inc. These compounds can be prepared, for example, by allowing N-bromosuccinimide to act on commercially available compounds in the presence of 2,2′-azobis(isobutyronitrile).
4-(Bromomethyl)-1-iodo-2-nitrobenzene can be prepared, for example, by brominating (4-iodo-3-nitro-phenyl)-methanol available from Combi-Blocks Inc. with hydrobromic acid.
Other benzyl bromides are commercially available, and those unavailable can be prepared, for example, by allowing N-bromosuccinimide to act on toluene having a corresponding substituent in the presence of 2,2′-azobis(isobutyronitrile), or by reducing benzaldehyde or methyl benzoate having a corresponding substituent with sodium borohydride or lithium aluminum hydride to obtain benzyl alcohol, and then brominating the benzyl alcohol with hydrobromic acid or phosphorus tribromide.
The reaction between the organic halide represented by the formula (II) and the protected amino acid can proceed in the presence of an organic solvent, a basic aqueous solution, and a phase transfer catalyst. Examples of the protected amino acid include p-chlorobenzaldehyde imine and benzophenone imine. Preferably, the protected amino acid may be p-chlorobenzaldehyde imine having the following structure.
Here, suitable examples of the organic solvent to be used include, but are not limited to, toluene, benzene, xylene, mesitylene, ethyl ether, isopropyl ether, tetrahydrofuran, dioxane, ethyl acetate, isopropyl acetate, cyclopentyl methyl ether, and methyl t-butyl ether.
The basic aqueous solution is preferably an aqueous solution of calcium hydroxide, cesium hydroxide, potassium hydroxide, or the like.
The phase transfer catalyst may be, for example, a Maruoka's reagent. The Maruoka's reagent is not limited, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide, (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide, (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide, (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide, or the like can be preferably used.
The reaction temperature for this reaction is preferably between −20° C. and 10° C., and the reaction time may be about 1 hour to about 60 hours.
After completion of the reaction, the mixture may be extracted with an organic solvent such as toluene, and optionally subjected to washing, drying, and filtration steps.
Next, to the reaction product is added a solvent, and the reaction product is reacted with an acid. Here, it is preferable to use an ether-based solvent as the solvent. Here, examples of the ether-based solvent include, but are not limited to, diethyl ether, tetrahydrofuran (THF), 2-methyltetrahydrofuran, dioxane, cyclopentyl methyl ether, glyme, and diglyme. In the present invention, tetrahydrofuran is particularly preferably used.
Examples of the acid include organic acids such as citric acid, acetic acid, trichloroacetic acid, trifluoroacetic acid, trifluoromethanesulfonic acid, toluenesulfonic acid, and methanesulfonic acid, and inorganic acids such as hydrochloric acid, sulfuric acid, nitric acid, and phosphoric acid.
The reaction is carried out at a temperature within the range of from 0° C. to 50° C.
The reaction time is about 1 to about 10 hours, more preferably 2 to 8 hours, and still more preferably 3 to 6 hours.
The amino group of the resulting compound is protected by a conventional method. The protecting group is not limited, but for example, a carbamate-based protecting group, an amide-based protecting group, or an alkyl protecting group is preferably used. Examples of the carbamate-based protecting group include tert-butoxycarbonyl group (Boc), benzyloxycarbonyl group (Cbz), 9-fluorenylmethyloxycarbonyl group (Fmoc), and 2,2,2-trichloroethoxycarbonyl group (Troc); examples of the amide-based protecting group include acetyl group and benzoyl group; and examples of the alkyl protecting group include benzyl group.
Next, the compound obtained is reacted with a boron compound in the presence of a palladium catalyst, an organophosphorus compound, and a base in a solvent.
Here, examples of the palladium catalyst include, but are not limited to, palladium(II) acetate, palladium(II) chloride, tris(dibenzylideneacetone)dipalladium(0), [1,1′-bis(diphenylphosphino)ferrocene]palladium(II) dichloride dichloromethane adduct, and tetrakis(triphenylphosphine)palladium (0).
Examples of the organophosphorus compound include, but are not limited to, triphenylphosphine, tricyclohexylphosphine, 1,1′-bis(diphenylphosphino)ferrocene, 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl, 2-dicyclohexylphosphino-2′,4′,6′-triisopropylbiphenyl, and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)biphenyl.
Examples of the base include, but are not limited to, potassium acetate, sodium acetate, sodium carbonate, cesium carbonate, potassium carbonate, and sodium hydrogen carbonate.
Examples of the boron compound include a boric acid ester or a boric acid amide, and the boron compound preferably refers to a compound represented by B(OR)3, B(NR)3, B(OR)2(NR), (RO)2B—B(OR)2, or B(OR) (NR)2 (R represents a linear or branched C1-C10 alkyl group, a phenyl group, or a benzyl group), or the like. Among these, a compound represented by (RO)2B—B(OR)2 is particularly preferably used. The “linear or branched C1-C10 alkyl group” as referred to herein may be any alkyl group having 1 to 10 carbon atoms, and is preferably a linear or branched C1-C8 alkyl group, and more preferably a linear or branched C1-C6 alkyl group. Examples of these groups include, but are not limited to, a methyl group, an ethyl group, an isopropyl group, and a butyl group. Examples of the boron compound typically include, but are not limited to, bis(pinacolato)diboron.
Examples of the solvent include, but are not limited to, ether-based solvents such as 1,4-dioxane, tetrahydrofuran, and 1,2-dimethoxyethane; hydrocarbon-based solvent such as toluene; and polar solvents such as N,N-dimethylformamide and dimethyl sulfoxide. Preferred solvents include dimethyl sulfoxide. The reaction temperature is, for example, 20° C. to 160° C., and preferably 60° C. to 120° C.
Next, the compound obtained is sequentially deprotected, and thus a desired compound can be produced. Deprotection is performed according to a conventional method, and can be performed by, for example, hydrolysis, catalytic hydrogenation, decarboxylation, or oxidation.
In each step of the production method, purification is performed according to a conventional method, and can be appropriately modified.
In particular, when the compound is a racemate, it can be used as it is, or for example, the optical purity of an R-isomer or an S-isomer may be increased in order to obtain a preferred compound for use for boron neutron capture therapy.
The optical resolution may be performed using an appropriate known method, and for example, in addition to a method in which the optical resolution (using α-chymotrypsin or the like) is performed through a hydrolysis step and an esterification step, a simplified method including a simplified step in which an acylase is used through a hydrolysis step may be adopted.
In the present invention, the method for producing a novel borono-phenylalanine derivative having a fused ring is not limited, and an ordinary amino acid synthesis method is used. The production method is performed according to the method for producing a 4-borono-phenylalanine derivative described above; first, for example, a protected amino acid is reacted with an organic halide represented by the following general formula (V) in the presence of an organic solvent, a basic aqueous solution, and a phase transfer catalyst.
In the formula (V), x may be any integer of 1 to 3. X3 represents halogen. An amino group or a hydroxyl group may be optionally protected. These can be obtained from commercially available products; for example, 2-amino-5-bromo-2,3-dihydro-1H-indene-2-carboxylic acid hydrochloride can be obtained from Enamine.
Other cyclic amino acids that are unavailable can be prepared by a Strecker reaction from indene or tetranone having a corresponding substituent. For example, one can be prepared by forming hydantoin with potassium cyanide and ammonium carbonate from a corresponding indene or tetranone, followed by hydrolysis with sodium hydroxide and subsequent decarboxylation with hydrochloric acid.
The 4-borono-phenylalanine derivative of the present invention can be used for a LAT1-selective agent in the form of the above-described compound or pharmaceutically acceptable salt as received, or in the form of a pharmaceutical preparation known to those skilled in the art in admixture with a pharmaceutically acceptable carrier, or in the form of encapsulation in micro/nanoparticles, or the like. Here, the LAT1-selective agent means that it is easily taken up by cells that express LAT1 and is hardly taken up by normal cells that express LAT2. LAT1 is abundantly expressed in cancer cells, and the effect of boron neutron capture therapy can be enhanced and damage to normal cells can be minimized if the compound has better selectivity of LAT1 compared to LAT2, which is expressed on the surface of normal cells. In the 4-borono-phenylalanine derivative of the present invention, the ratio of uptake into cells that express LAT1/uptake into cells that express LAT2 is preferably 2 times or more, more preferably 5 times or more, and still more preferably 10 times or more.
The borono-phenylalanine derivative of the present invention can be conveniently used for BNCT in the form of the above-described compound or pharmaceutically acceptable salt as received, or in the form of a pharmaceutical preparation known to those skilled in the art in admixture with a pharmaceutically acceptable carrier, or in the form of encapsulation in micro/nanoparticles, or the like.
Treatment with a pharmaceutical preparation of the borono-phenylalanine derivative of the present invention is carried out by administration via any suitable administration route in such a way that the borono-phenylalanine derivative accumulates in a target tumor. The borono-phenylalanine derivative is preferably concentrated in the tumor before irradiation, and a tumor blood ratio before irradiation is at least 1.5 or more:1, and preferably 2 or more:1. The borono-phenylalanine derivative may be administered at a time or continuously. In some cases, the borono-phenylalanine derivative may be administered separately. After the compound desirably accumulates in the tumor, the site is irradiated with an effective amount of low-energy neutron rays (for example, epithermal neutron rays). The site can be irradiated through the skin, or the site can be fully or partially exposed prior to irradiation. Administration of the borono-phenylalanine derivative and the subsequent irradiation may be repeated, as necessary. If desired, in order to reduce the tumor to a surgically possible extent, treatment with a borono-phenylalanine derivative may be performed and followed by a surgical procedure. Alternatively, after performing a surgical procedure, the remaining tumor is destroyed using a borono-phenylalanine derivative of the present invention. In another aspect, an appropriate amount of a borono-phenylalanine derivative is administered to a patient, followed by irradiation with an effective amount of 252 californium, which is a naturally occurring neutron radiant. Preferably, this is inserted into the tumor and then removed within a proper time.
Here, the type of tumor is not particularly limited, and brain tumors including glioblastoma and malignant glioma, head and neck cancers, malignant melanoma, breast cancer, prostate cancer, and the like can be particularly suitably targeted. In addition, epithelial cell cancers such as lung cancer, uterine cancer, kidney cancer, and liver cancer, various sarcomas, and the like can also be targeted.
The borono-phenylalanine derivative of the present invention may be administered orally and parenterally. In the case of parenteral administration, the borono-phenylalanine derivative may be administered intraarterially (for example, via carotid artery), intramuscularly, subcutaneously, intramedullary, intrathecally, intraventricularly, intravenously, intraperitoneally, or intranasally.
The pharmaceutical preparation may be formulated in any form such as powders, granules, fine granules, dry syrups, tablets, capsules, injections, and liquids and solutions. Depending on the dosage form, the pharmaceutical preparation may be administered to patients by a pharmaceutically known procedure in admixture with suitable additives and/or pharmaceutically acceptable carriers, alone, or in combination with other agents. Examples of the additives include excipients; disintegrators; binders; lubricants; diluents; buffers such as phosphoric acid, citric acid, succinic acid, acetic acid, other organic acids, and salts thereof; isotonizing agents; antiseptics; humectants; emulsifiers; dispersing agents; stabilizers; solubilizers; antioxidants such as ascorbic acid; low molecular weight (less than about 10 residues) polypeptides (for example, polyarginine or tripeptides); proteins (for example, serum albumin, gelatin, or immunoglobulin); hydrophilic polymers (for example, polyvinylpyrrolidone); amino acids (for example, glycine, glutamic acid, aspartic acid, or arginine); monosaccharides, disaccharides, and other carbohydrates (including cellulose or derivatives thereof, glucose, mannose, or dextrin); chelating agents (for example, EDTA); sugar alcohols (for example, mannitol or sorbitol); counter ions (for example, sodium); and/or nonionic surfactants (for example, polysorbate, poloxamer). The pharmaceutical preparation can be prepared by appropriately mixing with, or diluting and dissolving together with such pharmaceutical additives Carriers which can be preferably used are, but are not limited to, pharmaceutically inert aqueous carriers. Examples of such carriers include physiological saline, buffered physiological saline, dextrose, and water. In one embodiment of the present invention, the pharmaceutically acceptable carriers are pharmaceutically inactive. Suitable additives and/or pharmaceutically acceptable carriers are non-toxic to recipients at the dosages and concentrations employed. Particularly preferred for the pharmaceutical preparation are injections prepared with an aqueous carrier.
Techniques for formulation and administration are described, for example, in the latest edition of the Japanese Pharmacopoeia and its latest supplement, and in the final version of “REMINGTON'S PHARMACEUTICAL SCIENCES” (Maack Publishing Co., Easton, PA).
A pharmaceutical preparation of the borono-phenylalanine derivative of the present invention is an agent contained in an amount effective for the objective agent to achieve the intended purpose, and the “therapeutically effective amount” or “pharmacologically effective amount” is well recognized by a person ordinarily skilled in the art, and refers to an amount of an agent effective to produce a pharmacological result. Determination of a therapeutically effective dose is well known to a person ordinarily skilled in the art.
The therapeutically effective amount as referred to herein means an amount of the agent to alleviate the disease state by irradiation after the administration. The therapeutic effect and toxicity of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals. The dose is preferably in the range of circulating concentrations that include the ED50 with little or no toxicity. This dose varies within this range depending on the administration form used, the sensitivity of patients, and the administration route. As an example, the dose is appropriately chosen depending on the age or other conditions of patients, the kind of diseases, the kind of composites to be used, etc. Preferred doses can be, but are not limited to, 5 to 1000 mg/kg administered in one treatment. In particular, the dose of the derivative may be 5 to 500 mg, and more preferably 6 to 480 mg, per 1 kg of the weight of the subject to be treated.
The borono-phenylalanine derivatives of the present invention can also be prepared as agents containing radioisotopes. In the case of preparation as an agent containing a radioisotope, typically, but not limitingly, 18F can be used as an F atom contained in the compound, 131I or 123I can be used as an I atom contained in the compound, or 11C can be used as a C atom contained in the compound. These radioisotopes may be typically introduced into R1 to R4. Thus-obtained compounds can be used for, for example, RI inspection or nuclear medicine inspection. These include, but are not limited to, agents for scintigraphy tomography, single photon emission computed tomography (SPECT), and positron emission tomography (PET). That is, the 4-borono-phenylalanine derivative of the present invention containing radioactivity is administered to a subject as a PET agent or an SPECT agent, and an image is acquired before treatment, and thus information such as the bioaccumulation distribution of the derivative and the tumor tissue/normal tissue abundance ratio (T/N ratio) can be obtained. Based on these pieces of information, it is also possible to presume the therapeutic effect of BNCT and formulate a study or treatment plan. In addition, the administration mode and the like are in accordance with the contents described in the section of [Agent for boron neutron capture therapy (BNCT)].
The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto.
In the following examples, compounds were analyzed, and separated and purified using the following instruments and reagents.
What is shown in the LC/MS spectrum of the compound of the present invention was measured by the following method, and was represented by a retention time (unit: minute) and [M+H]+ or [M+Na]+.
To a solution of tert-butyl 2-((4-chlorobenzylidene)amino)propanoate (7.16 g, 26.7 mmol), 4-bromo-1-(bromomethyl)-2-fluorobenzene (8.64 g, 32.3 mmol), and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide (19.9 mg, 26.6 μmol) in toluene (56 mL) was added dropwise an 80% aqueous cesium hydroxide solution (24.9 g) at 5° C. or lower, and then the mixture was stirred at −5° C. for 42 hours. Water (30 mL) was added to the reaction mixture, and the mixture was extracted twice with toluene (50 mL), and then the organic phase was washed with saturated saline (50 mL). The organic phase was dried over anhydrous sodium sulfate and then concentrated under reduced pressure, affording a concentrate. The concentrate was used for the next step as received without purification.
The concentrate obtained was dissolved in tetrahydrofuran (50 mL), a 25% aqueous citric acid solution (203 g) was added thereto, and the mixture was stirred at room temperature for 3 hours. After completion of the reaction, the residue obtained by distilling off tetrahydrofuran under reduced pressure was washed with ethyl acetate (100 mL), and then the aqueous phase was adjusted to pH 8 or larger with potassium carbonate. The aqueous phase was extracted twice with ethyl acetate (100 mL), and then the organic phase was washed with saturated saline (100 mL). The organic phase was dried over anhydrous sodium sulfate and then concentrated under reduced pressure, and the resulting residue was purified by silica gel column chromatography (n-hexane:ethyl acetate=1:1 (v/v)), affording tert-butyl (S)-2-amino-3-(4-bromo-2-fluorophenyl)-2-methylpropanoate (6.17 g, yield 70%).
1H NMR (CDCl3); 1.44 (s, 9H, t-Bu), 2.80 (dd, J=9.5, 14.0 Hz, 1H, β-H), 3.15 (dd, J=6.0, 14.0 Hz, 1H, β-H), 3.66 (dd, J=5.5, 9.0 Hz, 1H, α-H), 7.12 (d, J=8.0 Hz, 1H, ArH), 7.42 (dd, J=1.5, 8.0 Hz, 1H, ArH), 7.99 (d, J=2.0 Hz, 1H, ArH).
tert-Butyl (S)-2-amino-3-(4-bromo-2-fluorophenyl)-2-methylpropanoate (5.00 g, 15.1 mmol) obtained in Step 1 was dissolved in acetonitrile (50 mL). Sodium carbonate (3.20 g, 30.2 mmol) in water (50 mL), Boc2O (3.93 g, 18.0 mmol) were added to the solution, and then the mixture was stirred at room temperature overnight. After the reaction, an aqueous phase obtained by concentrating acetonitrile under reduced pressure was extracted with ethyl acetate (50 mL), and the organic phase was dried over anhydrous magnesium sulfate, and then the solvent was concentrated under reduced pressure. The resulting residue was powdered with n-hexane, collected by filtration, and washed with n-hexane, affording tert-butyl (S)-3-(4-bromo-2-fluorophenyl)-2-((tert-butoxycarbonyl)amino)-2-methylpropanoate (4.90 g, yield 75%).
1H NMR (CDCl3); 1.46 (s, 21H, t-Bu×2+α-Me), 3.26-3.34 (m, 2H, β-H), 5.11 (brs, 1H, amide), 7.04 (t, J=8.0 Hz, 1H, ArH), 7.17-7.20 (m, 2H, ArH).
Pd(dba)2 (325 mg, 0.565 mmol) and tricyclohexylphosphine (222 mg, 0.791 mmol) were added to DMSO (49 mL), and the mixture was stirred at room temperature for 30 minutes. Bis(pinacolato)diboron (3.44 g, 13.6 mmol), potassium acetate (1.66 g, 17.0 mmol), and tert-butyl (S)-3-(4-bromo-2-fluorophenyl)-2-((tert-butoxycarbonyl)amino)-2-methylpropanoate (4.90 g, 11.3 mmol) were added thereto, and the mixture was stirred at 80° C. for 18 hours. The reaction mixture was cooled, and then ethyl acetate (49 mL) and distilled water (25 mL) were added thereto. After stirring for about 5 minutes, Celite filtration was conducted and the resulting filtrate was transferred to a separatory funnel. The aqueous phase was further extracted with ethyl acetate (25 mL), and the resulting organic phases were combined, washed with saturated saline (49 mL), dried over anhydrous magnesium sulfate, and then distilled off under reduced pressure, and the residue thus obtained was subjected to silica gel column chromatography, affording an intermediate.
An acetone solution (245 mL) of the obtained intermediate was added to a mixed liquid of sodium periodate (6.04 g, 28.3 mmol) and ammonium acetate (2.18 g, 28.3 mmol) in water (245 mL). The mixture was stirred at room temperature for 2 days. Acetone in the reaction liquid was distilled off under reduced pressure, and the resulting aqueous solution was extracted twice with ethyl acetate (123 mL). The organic phase was dried over anhydrous magnesium sulfate and then distilled off under reduced pressure, and the resulting residue was purified by silica gel column chromatography, affording (S)-(4-(3-(tert-butoxy)-2-((tert-butoxycarbonyl)amino)-2-methyl-3-oxopropyl)-3-fluorophenyl)boronic acid (2.38 g, yield 53%).
1H NMR (DMSO-d6); 1.08 (s, 3H, α-Me), 1.37 (s, 9H, t-Bu), 1.41 (s, 9H, t-Bu), 2.95 (d, J=13.5 Hz, 1H, β-H), 3.27 (d, J=13.5 Hz, 1H, β-H), 6.95 (brs, 1H, amide), 7.07 (t, J=7.5 Hz, 1H, ArH), 7.44-7.50 (m, 2H, ArH), 8.17 (s, 2H, B(OH)2).
(S)-(4-(3-(tert-Butoxy)-2-((tert-butoxycarbonyl)amino)-2-methyl-3-oxopropyl)-3-fluorophenyl)boronic acid (1.00 g, 2.52 mmol) was dissolved in trifluoroacetic acid (10 mL) and the solution was stirred for 3 hours. The reaction mixture was concentrated under reduced pressure, a small amount of distilled water was added thereto, and the mixture was neutralized with sodium carbonate. The resulting precipitate was collected by filtration and washed with cold water, affording (S)-2-amino-3-(4-borono-2-fluorophenyl)-2-methylpropanoic acid (0.36 g, yield 59%).
1H NMR (3.5% DCl in D2O); 1.68 (s, 3H, α-Me), 3.34 (s, 2H, β-H), 7.35 (t, J=7.5 Hz, 1H, ArH), 7.45-7.54 (m, 2H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.68 (s, 3H, α-Me), 3.34 (s, 2H, β-H), 7.35 (t, J=7.5 Hz, 1H, ArH), 7.45-7.54 (m, 2H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-methylbenzene.
1H NMR (3.5% DCl in D2O); 1.66 (s, 3H, α-Me), 2.34 (s, 3H, Ar-Me), 3.33-3.34 (m, 2H, β-H), 7.24 (d, J=7.5 Hz, 2H, ArH), 7.56 (d, J=7.5 Hz, 1H, ArH), 7.62 (s, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-methylbenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.66 (s, 3H, α-Me), 2.34 (s, 3H, Ar-Me), 3.33-3.34 (m, 2H, β-H), 7.24 (d, J=7.5 Hz, 2H, ArH), 7.56 (d, J=7.5 Hz, 1H, ArH), 7.62 (s, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-(trifluoromethyl)benzene.
1H NMR (3.5% DCl in D2O); 1.71 (s, 3H, α-Me), 3.50 (d, J=4.0 Hz, 2H, β-H), 7.48 (d, J=7.0 Hz, 2H, ArH), 7.92 (d, J=7.5 Hz, 1H, ArH), 8.08 (s, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-(trifluoromethyl)benzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.71 (s, 3H, α-Me), 3.50 (d, J=4.0 Hz, 2H, β-H), 7.48 (d, J=7.0 Hz, 2H, ArH), 7.92 (d, J=7.5 Hz, 1H, ArH), 8.08 (s, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-methoxybenzene.
1H NMR (3.5% DCl in D2O); 1.67 (s, 3H, α-Me), 3.28 (s, 2H, β-H), 3.85 (s, 3H, OMe), 7.26 (d, J=7.5 Hz, 1H, ArH), 7.34 (d, J=8.0 Hz, 1H, ArH), 7.35 (s, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-methoxybenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.67 (s, 3H, α-Me), 3.28 (s, 2H, β-H), 3.85 (s, 3H, OMe), 7.26 (d, J=7.5 Hz, 1H, ArH), 7.34 (d, J=8.0 Hz, 1H, ArH), 7.35 (s, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-chlorobenzene.
1H NMR (3.5% DCl in D2O); 1.67 (s, 3H, α-Me), 3.38 (d, J=14.5 Hz, 1H, β-H), 3.48 (d, J=14.0 Hz, 1H, β-H), 7.34 (d, J=8.0 Hz, 1H, ArH), 7.61 (dd, J=1.0, 7.5 Hz, 1H, ArH), 7.74 (d, J=1.5 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-chlorobenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.67 (s, 3H, α-Me), 3.38 (d, J=14.5 Hz, 1H, β-H), 3.48 (d, J=14.0 Hz, 1H, β-H), 7.34 (d, J=8.0 Hz, 1H, ArH), 7.61 (dd, J=1.0, 7.5 Hz, 1H, ArH), 7.74 (d, J=1.5 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 5-bromo-2-(bromomethyl)-1,3-difluorobenzene.
1H NMR (3.5% DCl in D2O); 1.65 (s, 3H, α-Me), 3.37 (s, 2H, β-H), 7.11 (d, J=8.0 Hz, 2H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 5-bromo-2-(bromomethyl)-1,3-difluorobenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.65 (s, 3H, α-Me), 3.37 (s, 2H, β-H), 7.11 (d, J=8.0 Hz, 2H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-chloro-5-fluorobenzene.
1H NMR (3.5% DCl in D2O); 1.66 (s, 3H, α-Me), 3.29 (s, 2H, β-H), 7.28 (dd, J=2.0, 10.0 Hz, 1H, ArH), 7.34 (d, J=5.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2,3-difluorobenzene.
1H NMR (3.5% DCl in D2O); 1.67 (s, 3H, α-Me), 3.36 (s, 2H, β-H), 7.11 (t, J=7.0 Hz, 1H, ArH), 7.36 (t, J=7.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-fluorobenzene.
1H NMR (3.5% DCl in D2O); 1.76 (s, 3H, α-Me), 3.23 (d, J=14.0 Hz, 1H, β-H), 3.23 (d, J=14.0 Hz, 1H, β-H), 7.05 (d, J=9.5 Hz, 1H, ArH), 7.16 (dd, J=1.0, 8.0 Hz, 1H, ArH), 7.68 (t, J=7.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-fluorobenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.76 (s, 3H, α-Me), 3.23 (d, J=14.0 Hz, 1H, β-H), 3.23 (d, J=14.0 Hz, 1H, β-H), 7.05 (d, J=9.5 Hz, 1H, ArH), 7.16 (dd, J=1.0, 8.0 Hz, 1H, ArH), 7.68 (t, J=7.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-methylbenzene.
1H NMR (3.5% DCl in D2O); 1.69 (s, 3H, α-Me), 2.45 (s, 3H, Me), 3.08 (d, J=14.0 Hz, 1H, β-H), 3.32 (d, J=14.0 Hz, 1H, β-H), 7.08-7.10 (m, 2H, ArH), 7.49 (d, J=6.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-methylbenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.69 (s, 3H, α-Me), 2.45 (s, 3H, Me), 3.08 (d, J=14.0 Hz, 1H, β-H), 3.32 (d, J=14.0 Hz, 1H, β-H), 7.08-7.10 (m, 2H, ArH), 7.49 (d, J=6.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that tert-butyl 2-((4-chlorobenzylidene)amino)propanoate was changed to tert-butyl 2-((4-chlorobenzylidene)amino)butanoate, and 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-iodobenzyl bromide.
1H NMR (3.5% DCl in D2O); 1.04 (t, J=7.5 Hz, 3H, α-CH2
The following compound was synthesized in the same manner as in Example 1 except that tert-butyl 2-((4-chlorobenzylidene)amino)propanoate was changed to tert-butyl 2-((4-chlorobenzylidene)amino)butanoate, 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-iodobenzyl bromide, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.04 (t, J=7.5 Hz, 3H, α-CH2CH3), 1.97-2.01 (m, 1H, α-CH2CH3), 2.15-2.19 (m, 1H, α-CH2CH3), 3.17 (d, J=14.0 Hz, 1H, β-H), 3.42 (d, J=14.0 Hz, 1H, β-H), 7.32 (d, J=8.0 Hz, 1H, ArH), 7.76 (d, J=8.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 5-bromo-2-(bromomethyl)benzonitrile.
1H NMR (3.5% DCl in D2O); 8.26 (d, J=1.5 Hz, 1H, ArH), 8.03 (dd, J=1.5, 7.5 Hz, 1H, ArH), 7.49 (d, J=7.5 Hz, 1H, ArH), 3.50 (d, J=16.5 Hz, 1H, CH2), 3.30 (d, J=16.5 Hz, 1H, CH2), 2.09 (s, 3H, CH3), 1.71 (s, 3H, CH3).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-chloro-5-fluorobenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.66 (s, 3H, α-Me), 3.29 (s, 2H, β-H), 7.28 (dd, J=2.0, 10.0 Hz, 1H, ArH), 7.34 (d, J=5.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-5-chloro-2-fluorobenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (TFA in D2O); 7.65 (dd, J=3.5, 6.0 Hz, 1H, ArH), 7.10 (dd, J=2.5, 9.5 Hz, 1H, ArH), 3.47 (d, J=15.0 Hz, 1H, CH2), 3.38 (d, J=15.0 Hz, 1H, CH2), 1.68 (s, 3H, CH3).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2,3-difluorobenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (TFA in D2O); 1.67 (s, 3H, α-Me), 3.36 (s, 2H, β-H), 7.11 (t, J=7.0 Hz, 1H, ArH), 7.36 (t, J=7.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-nitrobenzene.
1H NMR (TFA in D2O); 8.33 (d, J=1.5 Hz, 1H, ArH), 7.98 (d, J=8.0 Hz, 1H, ArH), 7.50 (dd, J=1.5, 8.0 Hz, 1H, ArH), 3.76 (d, J=14.0 Hz, 1H, CH2), 3.53 (d, J=14.0 Hz, 1H, CH2), 1.67 (s, 3H, CH3).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-(bromomethyl)-1-iodo-2-nitrobenzene.
1H NMR (TFA in D2O); 8.14 (s, 1H, ArH), 7.68 (d, J=1.5, 7.5 Hz, 1H, ArH), 7.63 (dd, J=1.5, 7.5 Hz, 1H, ArH), 3.50 (d, J=14.5 Hz, 1H, CH2), 3.29 (d, J=14.5 Hz, 1H, CH2), 1.68 (s, 3H, CH3).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-(bromomethyl)-1-iodo-2-nitrobenzene.
1H NMR (3.5% DCl in D2O); 1.74 (s, 3H, α-Me), 3.28 (d, J=14.0 Hz, 1H, β-H), 3.48 (d, J=14.5 Hz, 1H, β-H), 7.36 (s, 1H, ArH), 7.45 (d, J=8.0 Hz, 1H, ArH), 7.93 (d, J=7.5 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-nitrobenzene.
1H NMR (3.5% DCl in D2O); 1.47 (s, 3H, α-Me), 3.22 (d, J=15.5 Hz, 1H, β-H), 3.43 (d, J=15.5 Hz, 1H, β-H), 7.27 (s, 1H, ArH), 7.33 (d, J=7.5 Hz, 1H, ArH), 7.47 (d, J=7.5 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-5-chloro-2-fluorobenzene.
1H NMR (TFA in D2O); 7.65 (dd, J=3.5, 6.0 Hz, 1H, ArH), 7.10 (dd, J=2.5, 9.5 Hz, 1H, ArH), 3.47 (d, J=15.0 Hz, 1H, CH2), 3.38 (d, J=15.0 Hz, 1H, CH2), 1.68 (s, 3H, CH3).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 2-(benzyloxy)-4-bromo-1-(bromomethyl)benzene.
1H NMR (3.5% DCl in D2O); 1.77 (s, 3H, α-Me), 3.33 (d, J=14.5 Hz, 1H, β-H), 3.45 (d, J=14.0 Hz, 1H, β-H), 5.10 (d, J=11.5 Hz, 1H, ArCH2), 5.16 (d, J=12.0 Hz, 1H, ArCH2), 7.39-7.52 (m, 6H, ArH), 7.62-7.63 (m, 2H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 2-(benzyloxy)-4-bromo-1-(bromomethyl)benzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.77 (s, 3H, α-Me), 3.32 (d, J=14.0 Hz, 1H, β-H), 3.44 (d, J=13.5 Hz, 1H, β-H), 5.10 (d, J=12.0 Hz, 1H, ArCH2), 5.16 (d, J=11.5 Hz, 1H, ArCH2), 7.39-7.52 (m, 6H, ArH), 7.60-7.62 (m, 2H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-methoxybenzene.
1H NMR (3.5% DCl in D2O); 1.74 (s, 3H, α-Me), 3.14 (d, J=14.0 Hz, 1H, β-H), 3.38 (d, J=14.0 Hz, 1H, β-H), 3.86 (s, 3H, OCH3), 6.87 (d, J=2.0 Hz, 1H, ArH), 6.93 (d, J=7.5 Hz, 1H, ArH), 7.00 (dd, J=2.5, 8.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-methoxybenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.72 (s, 3H, α-Me), 3.11 (d, J=14.5 Hz, 1H, β-H), 3.36 (d, J=14.0 Hz, 1H, β-H), 3.84 (s, 3H, OCH3), 6.85 (s, 1H ArH), 6.91 (d, J=8.5 Hz, 1H, ArH), 7.00 (dd, J=2.0, 8.5 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-3-methylthiobenzene.
1H NMR (3.5% DCl in D2O); 1.39 (s, 3H, α-Me), 2.18 (s, 3H, SCH3), 3.04 (d, J=15.0 Hz, 1H, β-H), 3.14 (d, J=15.0 Hz, 1H, β-H), 6.92 (d, J=7.5 Hz, 1H, ArH), 7.26 (d, J=7.5 Hz, 1H, ArH), 7.40 (s, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-(difluoromethyl)benzene.
1H NMR (3.5% DCl in D2O); 1.82 (s, 3H, α-Me), 3.54-3.55 (m, 2H, β-H), 6.92-7.14 (m, 1H, CHF2). 7.47 (d, J=8.0 Hz, 1H, ArH), 7.96 (d, J=7.5 Hz, 1H, ArH), 8.05 (s, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-1-(bromomethyl)-2-(difluoromethyl)benzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.77 (s, 3H, α-Me), 3.49-3.50 (m, 2H, β-H), 6.92-7.13 (m, 1H, CHF2), 7.41 (d, J=8.0 Hz, 1H, ArH), 7.89 (d, J=7.5 Hz, 1H, ArH), 7.99 (s, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-chlorobenzene.
1H NMR (3.5% DCl in D2O); 1.68 (s, 3H, α-Me), 3.13 (d, J=14.0 Hz, 1H, β-H), 3.35 (d, J=14.5 Hz, 1H, β-H), 7.20 (dd, J=1.0, 7.5 Hz, 1H, ArH), 7.30 (d, J=1.0 Hz, 1H, ArH), 7.52 (d, J=7.5 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-chlorobenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.69 (s, 3H, α-Me), 3.14 (d, J=14.0 Hz, 1H, β-H), 3.36 (d, J=14.0 Hz, 1H, β-H), 7.21 (d, J=8.0 Hz, 1H, ArH), 7.30 (s, 1H, ArH), 7.53 (d, J=7.5 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-(trifluoromethyl)benzene.
1H NMR (3.5% DCl in D2O); 1.71 (s, 3H, α-Me), 3.24 (d, J=14.5 Hz, 1H, β-H), 3.44 (d, J=14.5 Hz, 1H, β-H), 7.51 (d, J=8.0 Hz, 1H, ArH), 7.61-7.63 (m, 2H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2-(trifluoromethyl)benzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.69 (s, 3H, α-Me), 3.23 (d, J=14.5 Hz, 1H, β-H), 3.44 (d, J=14.5 Hz, 1H, β-H), 7.50 (d, J=8.0 Hz, 1H, ArH), 7.60-7.61 (m, 2H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-2,5-difluorobenzene, and (R)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide was changed to (S)-4,4-dibutyl-2,6-bis(3,4,5-trifluorophenyl)-4,5-dihydro-3H-dinaphtho[2,1-c:1′,2′-e]azepinium bromide.
1H NMR (3.5% DCl in D2O); 1.64 (s, 3H, α-Me), 3.36 (s, 2H, β-H), 7.31-7.33 (m, 2H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that tert-butyl 2-((4-chlorobenzylidene)amino)propanoate was changed to tert-butyl 2-((4-chlorobenzylidene)amino)butanoate.
1H NMR (3.5% DCl in D2O); 1.02 (t, J=7.5 Hz, 3H, α-CH2CH3), 1.92-2.00 (m, 1H, α-CH2CH3), 2.14-2.22 (m, 1H, α-CH2CH3), 3.35 (s, 2H, β-H), 7.36 (d, J=7.5 Hz, 1H, ArH), 7.47 (d, J=11.0 Hz, 1H, ArH), 7.53 (dd, J=1.0, 7.0 Hz, 1H, ArH).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 1-bromo-4-(bromomethyl)-3-methylthiobenzene, and this was oxidized with m-chloroperbenzoic acid to sulfone before borylation.
Retention time: 0.19 min
HRMS (ESI, [M+H]+), calcd for 302.0870; found: 302.1029.
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 5-bromo-2-(bromomethyl)pyridine, and tert-butyl 2-((4-chlorobenzylidene)amino)propanoate was changed to N-(diphenylmethylene)glycine tert-butyl.
1H NMR (D2O); 8.82 (brs, 1H, ArH), 8.73 (dd, J=7.0, 9.5 Hz, 1H, ArH), 7.98 (d, J=8.0 Hz, 1H, ArH), 4.49 (t, J=7.5 Hz, 1H, CH), 3.65 (d, J=7.5 Hz, 2H, CH2).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 5-bromo-2-(bromomethyl)pyridine.
1H NMR (D2O); 8.87 (d, J=3.5 Hz, 1H, ArH), 8.77 (dd, J=2.5, 7.5 Hz, 1H, ArH), 7.96 (dd, J=3.0, 7.5 Hz, 1H, ArH), 3.65 (d, J=15.0 Hz, 1H, CH2), 3.61 (d, J=15.0 Hz, 1H, CH2), 1.61 (s, 3H, CH3).
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 5-bromo-2-(bromomethyl)pyrimidine.
Retention time: 0.19 min
HRMS (ESI, [M+H]+), calcd for 212.0843; found: 212.0982.
The following compound was synthesized in the same manner as in Example 1 except that 4-bromo-1-(bromomethyl)-2-fluorobenzene was changed to 4-bromo-2-(bromomethyl)thiophene.
Retention time: 0.26 min
HRMS (ESI, [M+H]+), calcd for 216.0502; found: 216.0161
Under a nitrogen atmosphere, methyl (R)-2-((tert-butoxycarbonyl) amino)-3-iodopropanoate (2.98 g, 9.05 mmol) was added to the mixture of zinc powder (710 mg, 10.9 mmol), N,N-dimethylformamide (12 mL) and trimethylsilyl chloride (98.3 mg, 0.905 mmol) under ice cooling. When heat generation had subsided, 2,5-dibromo-3-fluoropyridine (3.00 g, 11.8 mmol) and bis(triphenylphosphine)palladium(II) dichloride (318 mg, 0.453 mmol) were added, and the mixture was stirred at 80° C. for 1.5 hours. The reaction mixture was cooled to room temperature, filtered through Celite to remove a zinc residue, and the residue was washed with ethyl acetate (30 mL). To the resulting filtrate was added a saturated aqueous ammonium chloride solution (10 mL), and the mixture was extracted with ethyl acetate (30 mL). The organic phase was washed with saturated saline (10 mL), dried over anhydrous sodium sulfate, and then concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (hexane/ethyl acetate=8/1), affording methyl (S)-3-(5-bromo-3-fluoropyridin-2-yl)-2-((tert-butoxycarbonyl)amino)propanoate (1.71 g, 4.55 mmol, 50%) as a yellow oil.
Retention time: 2.52 min
HRMS (ESI, [M+Na]+), calcd for 399.0332; found: 399.0714.
Under a nitrogen atmosphere, bis(pinacolato)diboron (2.08 g, 8.19 mmol), Pd(dba)2 (131 mg, 0.228 mmol), tricyclohexylphosphine (128 mg, 0.455 mmol), potassium acetate (1.34 g, 13.7 mmol), and 1,4-dioxane (30 mL) were added to methyl (S)-3-(5-bromo-3-fluoropyridin-2-yl)-2-((tert-butoxycarbonyl)amino)propanoate (1.71 g, 4.55 mmol), and the mixture was stirred at 80° C. for 6 hours. The reaction mixture was filtered through Celite to remove a palladium residue, and the residue was washed with ethyl acetate (30 mL). Water (10 mL) was added to the resulting filtrate, and the mixture was extracted with ethyl acetate (30 mL). The organic phase was washed with saturated saline (10 mL), dried over anhydrous sodium sulfate, and then concentrated under reduced pressure. The resulting residue was passed through alumina (neutral) and distilled off with ethyl acetate for rough purification, affording a crude product (2.85 g) containing methyl (S)-2-((tert-butoxycarbonyl)amino)-3-(3-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl)propanoate as a yellow oil.
Sodium periodate (6.82 g, 31.9 mmol) and ammonium acetate (2.46 g, 31.9 mmol) were added to an acetone/water (2/1, 30 mL) solution of the crude product (2.85 g) obtained, and the mixture was stirred at room temperature for 15 hours. The insoluble matter was collected by filtration, and then acetone in the resulting filtrate was distilled off under reduced pressure. The resulting residue was extracted twice with ethyl acetate (50 mL). The organic phase was washed with saturated saline (10 mL), dried over anhydrous sodium sulfate, and then concentrated under reduced pressure. The resulting residue was powdered with diisopropyl ether (5 mL), affording a crude product containing (S)-(6-(2-((tert-butoxycarbonyl)amino)-3-methoxy-3-oxopropyl)-5-fluoropyridin-3-yl)boronic acid (300 mg) as a light yellow powder.
Concentrated hydrochloric acid (4.4 mL) was added to the crude product (300 mg) obtained, and the mixture was stirred at 70° C. for 22 hours. Water (20 mL) was added to the reaction mixture, and the aqueous phase was washed with dichloromethane (20 mL×4). The aqueous phase was concentrated under reduced pressure, affording (S)-2-amino-3-(5-borono-3-fluoropyridin-2-yl)propanoic acid hydrochloride (222 mg, 0.738 mmol, 16% for 3 steps) as a light yellow solid.
1H NMR (D2O); 8.67 (s, 1H, ArH), 8.33 (d, J=8.5 Hz, 1H, ArH), 4.39 (dd, J=5.5, 8.0 Hz, 1H, CH), 3.65 (dd, J=5.5, 15.0 Hz, 1H, CH2), 3.59 (dd, J=8.0, 15.0 Hz, 1H, CH2).
The following compound was synthesized in the same manner as in Example 48 except that 2,5-dibromo-3-fluoropyridine was changed to 5-bromo-2-iodo-3-methylpyridine, and Pd(PPh3)2Cl2 was changed to Pd2 (dba)3 and tri(ortho-tolyl)phosphine.
1H NMR (D2O); 8.68 (s, 1H, ArH), 8.61 (s, 1H, ArH), 4.36 (dd, J=5.0, 10.0 Hz, 1H, CH), 3.66 (dd, J=5.0, 14.5 Hz, 1H, CH2), 3.60 (dd, J=10.0, 14.5 Hz, 1H, CH2), 2.54 (s, 3H, CH3).
The following compound was synthesized in the same manner as in Example 48 except that 2,5-dibromo-3-fluoropyridine was changed to 2,5-dibromo-3-chloropyridine.
1H NMR (D2O); 8.72 (d, J=1.5 Hz, 1H, ArH), 8.53 (d, J=1.5 Hz, 1H, ArH), 4.42 (dd, J=6.0, 8.5 Hz, 1H, CH), 3.71 (dd, J=6.0, 15.0 Hz, 1H, CH2), 3.63 (dd, J=8.5, 15.0 Hz, 1H, CH2).
The following compound was synthesized in the same manner as in Example 48 except that 2,5-dibromo-3-fluoropyridine was changed to 5-bromo-2-iodo-4-methylpyridine.
1H NMR (D2O); 8.56 (s, 1H, ArH), 7.77 (s, 1H, ArH), 4.43 (t, J=7.5 Hz, 1H, CH), 3.55 (d, J=7.5 Hz, 2H, CH2), 2.64 (s, 3H, CH3).
A mixed liquid of 2-amino-5-bromo-2,3-dihydro-1H-indene-2-carboxylic acid hydrochloride (0.5 g, 1.7 mmol), methanol (5 mL), and concentrated sulfuric acid (0.25 mL) was heated to reflux for 16 hours. The reaction mixture was added to saturated aqueous sodium bicarbonate solution (100 mL), and the resulting mixture was extracted with dichloromethane (50 mL×2), and then the organic phase was washed with semi-saturated saline (100 mL). The organic phase was dried over anhydrous sodium sulfate and then concentrated under reduced pressure, affording methyl 2-amino-5-bromo-2,3-dihydro-1H-indene-2-carboxylate (0.43 g, yield 93.6%) as a light brown oil.
To a mixture of methyl 2-amino-5-bromo-2,3-dihydro-1H-indene-2-carboxylate (0.43 g, 1.6 mmol), dichloromethane (15 mL), and triethylamine (0.45 mL) was added dropwise a solution of di-tert-butyl dicarbonate (0.55 mL) in dichloromethane (3 mL) over 2 minutes under ice cooling.
The reaction mixture was stirred at room temperature overnight, then added to dichloromethane (100 mL), and washed with semi-saturated saline (100 mL×2). The organic phase was dried over anhydrous sodium sulfate and then concentrated under reduced pressure, and the resulting residue was purified by silica gel column chromatography (hexane/ethyl acetate), affording methyl 5-bromo-2-(tert-butoxycarbonyl)amino-2,3-dihydro-1H-indene-2-carboxylate (0.26 g, yield 43.9%) as a white solid.
1H NMR (CDCl3); 1.42 (s, 9H), 3.04-3.51 (m, 4H), 3.76 (s, 3H), 5.07 (s, 1H), 6.97-7.33 (m, 4H).
A mixed liquid of methyl 5-bromo-2-(tert-butoxycarbonyl)amino-2,3-dihydro-1H-indene-2-carboxylate (0.24 g, 0.65 mmol), bis(pinacolato)diboron (0.2 g), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (0.03 g), potassium acetate (0.1 g), and dimethyl sulfoxide (3 mL) was purged with nitrogen, and then stirred at 95° C. for 8 hours. Water (100 mL) was added to the reaction mixture, then the mixture was extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100 mL), then dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (hexane/ethyl acetate), affording methyl 2-(tert-butoxycarbonyl)amino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaboran-2-yl)-2,3-dihydro-1H-indene-2-carboxylate (0.25 g, yield 92.2%) as a colorless amorphous.
Sodium periodate (0.19 g) and ammonium acetate (0.07 g) were added to the mixture of methyl 2-(tert-butoxycarbonyl)amino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,3-dihydro-1H-indene-2-carboxylate (0.09 g, 0.22 mmol), acetone (7 mL), and water (3.5 mL), and the mixture was stirred at room temperature overnight. The reaction mixture was added to ethyl acetate (100 mL), and washed with semi-saturated saline (150 mL×2). The organic phase was dried over anhydrous sodium sulfate and then concentrated under reduced pressure, affording methyl 2-(tert-butoxycarbonyl)amino-5-borono-2,3-dihydro-1H-indene-2-carboxylate (0.08 g, yield 99%) as a white amorphous.
To a solution of methyl 5-borono-2-(tert-butoxycarbonyl)amino-2,3-dihydro-1H-indene-2-carboxylate (0.17 g, 0.5 mmol) in methanol (2 mL) was added a 1 M aqueous sodium hydroxide solution (2 mL) under ice cooling. The mixture was stirred at room temperature for 3 hours. The reaction mixture was adjusted to about 3 with 1 M hydrochloric acid under ice cooling, and then the reaction mixture was extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100 mL), then dried over anhydrous sodium sulfate, and then concentrated under reduced pressure, affording 5-borono-2-(tert-butoxycarbonyl)amino-2,3-dihydro-1H-indene-2-carboxylic acid (0.15 g, yield 93.4%) as a white amorphous.
Trifluoroacetic acid (1.44 mL) was added to 5-borono-2-(tert-butoxycarbonyl)amino-2,3-dihydro-1H-indene-2-carboxylic acid (0.15 g, 0.47 mmol), and the mixture was stirred at room temperature for 2 hours. The reaction mixture was concentrated under reduced pressure, then toluene (3 mL) was added thereto, and the resulting mixture was further concentrated under reduced pressure. The resulting residue was powdered with dichloromethane, affording trifluoroacetate of 2-amino-5-borono-2,3-dihydro-1H-indene-2-carboxylic acid (0.13 g, yield 82.6%) as a white powder.
1H NMR (D2O); 3.39 (d, J=18 Hz, 2H, CH2), 3.79 (d, J=18 Hz, 2H, CH2), 7.38 (d, J=7.5 Hz, 1H, ArH), 7.67-7.68 (m, 2H, ArH).
The following compound was synthesized in the same manner as in Example 52 except that 2-amino-5-bromo-2,3-dihydro-1H-indene-2-carboxylic acid hydrochloride was changed to 2-amino-7-bromo-1,2,3,4-tetrahydronaphthalene-2-carboxylic acid.
1H NMR (D2O); 2.17-2.20 (m, 1H, CH2), 2.38-2.42 (m, 1H, CH2), 2.90-3.11 (m, 3H, CH2), 3.47-3.50 (m, 1H, CH2), 7.20-7.29 (m, 2H, ArH), 7.54-7.60 (m, 1H, ArH).
HEK 293 cells that stably highly express human LAT1 and LAT2 respectively were prepared according to the method described in the article of Khunweeraphong, N et al. (Journal of Pharmacology Science, 2012, vol. 119, pp 368-380). A shuttle vector DNA (LAT1: EX-H 4509-M02, LAT2: EX-U0514-M02, manufactured by GeneCopoeia) having ampicillin and neomycin resistance markers and having full-length cDNA of human LAT1 or LAT2 inserted under the promoter of cytomegalovirus (CMV) was constructed. This vector was transfected into HEK 293 cells by a method using Lipofectamine (registered trademark) 2000 (Invitrogen) in accordance with the manufacturer's instructions. Thereafter, the stably expressing cell clone of the transgene was selected by the limiting dilution method in the presence of 0.9 mg/mL Geneticin (registered trademark), and a cell clone in which the uptake of L-boronophenylalanine (L-BPA) was enhanced by about 2 to about 5 times as compared with HEK 293 cells before transfection was obtained. This cell was passaged and used for evaluation of LAT1 and LAT2-selective uptake.
As a method for evaluating cell uptake, the same method as in the article of Khunweeraphong, N et al. (Journal of Pharmacology Science, 2012, vol. 119, pp 368-380) was used. Each of the cells obtained in (1) was used for evaluation. Radioactive isotopes were not used, a substrate concentration was 0.1 mM, and 2 mM BCH (2-amino-2-norbonanecarboxylic acid) was used as an LAT1 and LAT2 inhibitor. The reacted cells were collected with 0.05% Tween 20 and the concentration of the 4-borono-phenylalanine derivative in the cell fluid was determined. Quantification of the 4-borono-phenylalanine derivative in cells was performed in accordance with the method described in the article of Hattori, Y, et al. (Sensors 2017, 17, 2436), using 2-(2-hydroxyphenyl)pyridine (boron sensor 5) as a boron sensor. In the evaluation of cell uptake, in consideration of variations between experiments, the cell uptake was evaluated as a value (LAT1 selectivity) relative to the uptake of L-BPA as a comparative object performed on the same day.
LAT1 selectivity=(quantitative value of uptake of the compound in LAT1 cells/quantitative value of uptake of control L-BPA in LAT1 cells)/(quantitative value of uptake of the compound in LAT2 cells/quantitative value of uptake of control L-BPA in LAT2 cells)
That is, it is as follows.
As a result of the uptake evaluation performed by comparing the compounds of Examples with L-BPA, a plurality of compounds showing high values of LAT1 selectivity were found. Among them, in particular, the following compounds showed extremely high values, namely twice or more:
Test for Evaluating Uptake into Tumor Cells
On a 100 mm dish are seeded 1.5×106 human tongue cancer cells (SAS cells), human glioma cells (A172 cells), or human breast cancer cells (MCF-7), and pre-culture is performed at 37° C. in an atmosphere of 5% CO2 for 24 hours. The culture fluid is removed by suction, a culture fluid containing 1 mM of the agent of each Example or Reference Example is added, and then exposed and cultured at 37° C. in an atmosphere of 5% CO2 for 3 hours. After the culture fluid is removed by suction, the cells are washed once using PBS, and then trypsinized to recover the cells. The number of the cells recovered is counted and packed by centrifugation, then HClO4 (60%, 0.3 ml) and H2O2 (31%, 0.6 mL) are added, the mixture is heated at 75° C. overnight, and an ashed solution is thereby prepared. The ashed solution is filled up to 5 mL with pure water, and then filtered using a 5C filter paper, the boron concentration in the solution is measured using 710 ICP-OES manufactured by Agilent Technologies Inc., and the amount (μg) of boron per 107 cells is thereby determined. In the evaluation of cell uptake, in consideration of variations between experiments, the cell uptake is evaluated as a value relative to the uptake of L-BPA of Reference Example as a comparative object performed on the same day.
In a beaker, 17.6 mL of 1 mol/L NaOH was put, and 24 mL of water for injection was further added and mixed. To this was added 2.52 g of D-sorbitol. After confirming complete dissolution, 17.1 mL of 1 mol/L hydrochloric acid was added to adjust the pH to 7.4 to 7.8. The mixture was transferred to a measuring cylinder, the beaker was washed with water for injection, and the washing liquid was transferred to the measuring cylinder. This operation was repeated as much as possible, and the volume of the liquid in the measuring cylinder was increased to 80 mL while the beaker was washed. The mixture was filtration-sterilized with a filter (Millex GV, pore size: 0.22 μm, Merck, sterilized disposable product), affording a 3.15% D-sorbitol solution.
On the other hand, 10.1 mL of a 1 mol/L aqueous sodium hydroxide solution was put in a beaker, and 13.8 mL of water for injection was further added and mixed. To this were added 2.30 g of test substance and 1.45 g of D-sorbitol. After confirming complete dissolution, 1.36 mL of 1 mol/L hydrochloric acid was added to adjust the pH to 7.4 to 7.8. The mixture was transferred to a measuring cylinder, the beaker was washed with water for injection, and the washing liquid was transferred to the measuring cylinder. This operation was repeated as much as possible, and the volume of the liquid in the measuring cylinder was increased to 46 mL while washing the beaker. The mixture was filtration-sterilized with a filter (Millex GV, pore size: 0.22 μm, Merck, sterilized disposable product), affording a liquid with a concentration of 50 mg/mL.
An animal was placed in a fixation device (Bollman cage) and injected into the tail vein at a rate of 1 mL/kg/min with a microprocessor single syringe pump (Pump 11 Elite, Harvard Apparatus Inc.) using a 10 mL polypropylene syringe filled with the administration liquid and a 24 gauge indwelling needle (Surflow F&F, Terumo Corporation, all sterilized disposable products). At doses of 250, 500, and 1000 mg/kg, 5 animals in each group were administered, and after completion of the administration, the animals were returned to the breeding cages.
The observation period was 8 days including the administration day. The administration day was defined as Day 1, the day following the administration day was defined as Day 2, and the subsequent days are indicated based on those definitions. The observation frequency was set to 6 times (immediately before administration, 5 minutes, 30 minutes, 60 minutes, 2 hours, and 4 hours after the end of administration) on the administration day, and once a day on and after the day following the administration day. Individual observation was performed from the outside of the cages, and animals suspected of having abnormality were taken out from the cages and observed. The time of body weight measurement was set to Day 1 (before administration), Day 4, and Day 8.
As a result of this test, with the compound (S)-2-amino-3-(4-borono-2-fluorophenyl)-2-methylpropanoic acid and the compound (S)-2-amino-3-(4-borono-2-chlorophenyl)-2-methylpropanoic acid, no serious change due to the administration of the test substance was observed in any of the administration groups throughout the entire observation period.
Human pancreatic cancer cells T3M-4 cells are cultured using a HAM-F12 medium. Cells are detached with trypsin solution and collected by centrifugation. Cells are suspended in PBS (-) to 4×106 cells/100 μL. The cell suspension is injected (100 μL/mouse) subcutaneously into the right lower limb of BALB/c nu/nu mice (male, 4 weeks old) using a 26 G needle. Thereafter, the size of the tumor formed is visually examined for 3 to 4 weeks, and when the tumor size reaches about 4 mm to about 10 mm, the sample is used for distribution experiment. The administration agent is correspondingly prepared with an agent, for example, by adding fructose or sorbitol, and physiological saline or PBS (-) is used as the diluent. To tumor-bearing mice, 100 μL of the agent with a sample concentration of 10 mg/mL as a standard is administered at their tail vein. The mice are sacrificed over time and dissected, and organ weights are measured. The extracted organ is ashed with nitric acid, and the amount of boron is quantified by ICP-MS or ICP-OES.
As a method for evaluating Km, the cell uptake method described in the article of Khunweeraphong, N, et al. (Journal of Pharmacology Science, 2012, vol. 119, pp 368-380) is used, and the uptake time is 2 to 3 minutes. At this time, radioactive isotopes are not used, and cells reacted at a plurality of substrate concentrations are recovered with 0.05% Tween 20, and the concentration of the boron compound in the obtained cell fluid is determined. As the cells, HEK 293 cells that stably express Human LAT1 transporter or Human LAT2 transporter established according to (Journal of Pharmacology Science, 2012, vol. 119, pp 368-380) are used.
Quantification of the boron compound in cells is performed in accordance with the method described in the article of Hattori, Y, et al. (Sensors 2017, 17, 2436), using 2-(2-hydroxyphenyl)pyridine (boron sensor 5) as a boron sensor. In the evaluation, Km is calculated using Lineweaver-Burk plot.
After making LAT1-enhanced HEK 293 cells uptake the substrate, an effluent is added and the boron compound remaining in the cells is measured. HBSS (Na+-Free) and 0.05 mM leucine are used as the LAT1 effluent at a substrate concentration of 0.1 mM, and the concentration of the boron compound in the cell fluid obtained by collecting the reacted cells with 0.05% Tween 20 is determined. The efflux time is set to 1 to 10 minutes.
Quantification of the boron compound in cells is performed in accordance with the method described in the article of Hattori, Y, et al. (Sensors 2017, 17, 2436), using 2-(2-hydroxyphenyl)pyridine (boron sensor 5) as a boron sensor.
In the evaluation of cell uptake, the cell uptake is evaluated as a value relative to the cell fluid before efflux as a comparative object.
An appropriate amount of the compound of each Example is weighed and dissolved in water. Hydrochloric acid or an aqueous sodium hydroxide solution is added dropwise, and the mixture is made neutral while checking with a pH test paper. The agent prepared is analyzed by ICP to calculate the boron concentration, which is taken as the sample concentration.
Using commercially available pooled human liver microsomes, a target compound is reacted for a prescribed period of time, and the reacted sample is compared to an unreacted sample to calculate a residual ratio. Then, the degree of hepatic metabolism is evaluated.
A reaction (oxidative reaction) is performed at 37° C. for 0 minutes or 30 minutes in 0.2 mL of a buffer (50 mmol/L tris-HCl pH 7.4, 150 mmol/L potassium chloride, 10 mmol/L magnesium chloride) containing 0.5 mg protein/mL of human liver microsome in the presence of 1 mmol/L NADPH. After the reaction, 50 μL of the reaction liquid is added to 100 μL of a methanol/acetonitrile=1/1 (v/v) solution, mixed, and centrifuged at 3000 rpm for 15 minutes. The test compound in the centrifugal supernatant is quantified by LC/MS-MS, and the residual amount of the test compound after the reaction is calculated with the amount of the compound at 0 minute reaction defined as 100%.
Using various commercially available frozen hepatocytes, a target compound is reacted for a prescribed period of time, and the reacted sample is compared to an unreacted sample to calculate a residual ratio. Then, the degree of hepatic metabolism is evaluated.
A reaction is carried out at 37° C. for 0, 1, or 2 hours in William E culture medium containing 1.0×106 cells/mL of frozen hepatocytes. After the reaction, 120 μL of a methanol/acetonitrile=1/1 (v/v) solution is added to 30 μL of the reaction liquid, mixed, and centrifuged at 3000 rpm for 15 minutes. The test compound in the centrifugal supernatant is quantified by LC/MS-MS, and the residual amount of the test compound after the reaction is calculated with the amount of the compound at 0 minute reaction defined as 100%.
The serum protein unbinding rate of the compound of the invention is measured using various serums.
The reaction conditions are as follows. Evaluation method: equilibrium dialysis; reaction time: 24 h; reaction temperature: 37° C.; concentration of the compound of the invention: 2 μg/mL
A serum sample having the above-mentioned compound concentration is prepared by adding a test liquid to a serum and stirring the mixture. The serum sample is added to one equilibrium dialysis cell and a phosphate buffered saline (PBS) is added to another equilibrium dialysis cell, and then equilibrium dialysis is performed at 37° C. for 24 hours. The amount of the compound in the sample collected from each cell is measured by LC/MS-MS.
The compound of the present invention is dissolved in water or a buffer solution to prepare an injection. In the case of being insoluble in water, an aqueous solution of sodium hydroxide is added and the compound is dissolved, and the mixture is neutralized with hydrochloric acid to prepare an injection. From the osmotic relationship, physiological saline, phosphate buffer, monosaccharides, disaccharides, or other carbohydrates may also be added.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2021-105562 | Jun 2021 | JP | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/JP2022/018258 | 4/20/2022 | WO |
