Information
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Patent Application
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20040002067
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Publication Number
20040002067
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Date Filed
December 21, 200122 years ago
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Date Published
January 01, 200420 years ago
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CPC
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US Classifications
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International Classifications
- C12Q001/68
- G06F019/00
- G01N033/48
- G01N033/50
- C12M001/34
Abstract
Methods and compositions for the identification of breast cancer progression signatures are provided. The signature profiles are identified based upon multiple sampling of reference breast tissue samples from independent cases of breast cancer and provide a reliable set of molecular criteria for identification of cells as being in one or more particular stages of breast cancer.
Description
FIELD OF THE INVENTION
[0001] The invention relates to the identification and use of gene expression profiles, or patterns, involved in breast cancer progression. The gene expression profiles, whether embodied in nucleic acid expression, protein expression, or other expression formats, are used in the study and/or diagnosis of cells and tissue during breast cancer progression as well as for the study and/or determination of prognosis of a patient. When used for diagnosis or prognosis, the profiles are used to predict the status and/or phenotype of cells and tissues relative to breast cancer and the treatment thereof.
BACKGROUND OF THE INVENTION
[0002] Breast cancer is by far the most common cancer among women. Each year, more than 180,000 and 1 million women in the U.S. and worldwide, respectively, are diagnosed with breast cancer. Breast cancer is the leading cause of death for women between ages 50-55, and is the most common non-preventable malignancy in women in the Western Hemisphere. An estimated 2,167,000 women in the United States are currently living with the disease (National Cancer Institute, Surveillance Epidemiology and End Results (NCI SEER) program, Cancer Statistics Review (CSR), www-seer.ims.nci.nih.gov/Publications/CSR1973 (1998)). Based on cancer rates from 1995 through 1997, a report from the National Cancer Institute (NCI) estimates that about 1 in 8 women in the United States (approximately 12.8 percent) will develop breast cancer during her lifetime (NCI's Surveillance, Epidemiology, and End Results Program (SEER) publication SEER Cancer Statistics Review 1973-1997). Breast cancer is the second most common form of cancer, after skin cancer, among women in the United States. An estimated 250,100 new cases of breast cancer are expected to be diagnosed in the United States in 2001. Of these, 192,200 new cases of more advanced (invasive) breast cancer are expected to occur among women (an increase of 5% over last year), 46,400 new cases of early stage (in situ) breast cancer are expected to occur among women (up 9% from last year), and about 1,500 new cases of breast cancer are expected to be diagnosed in men (Cancer Facts & Figures 2001 American Cancer Society). An estimated 40,600 deaths (40,300 women, 400 men) from breast cancer are expected in 2001. Breast cancer ranks second only to lung cancer among causes of cancer deaths in women. Nearly 86% of women who are diagnosed with breast cancer are likely to still be alive five years later, though 24% of them will die of breast cancer after 10 years, and nearly half (47%) will die of breast cancer after 20 years.
[0003] Every woman is at risk for breast cancer. Over 70 percent of breast cancers occur in women who have no identifiable risk factors other than age (U.S. General Accounting Office. Breast Cancer, 1971-1991: Prevention, Treatment and Research. GAO/PEMD-92-12; 1991). Only 5 to 10% of breast cancers are linked to a family history of breast cancer (Henderson I C, Breast Cancer. In: Murphy G P, Lawrence W L, Lenhard R E (eds). Clinical Oncology. Atlanta, Ga.: American Cancer Society; 1995:198-219).
[0004] Each breast has 15 to 20 sections called lobes. Within each lobe are many smaller lobules. Lobules end in dozens of tiny bulbs that can produce milk. The lobes, lobules, and bulbs are all linked by thin tubes called ducts. These ducts lead to the nipple in the center of a dark area of skin called the areola. Fat surrounds the lobules and ducts. There are no muscles in the breast, but muscles lie under each breast and cover the ribs. Each breast also contains blood vessels and lymph vessels. The lymph vessels carry colorless fluid called lymph, and lead to the lymph nodes. Clusters of lymph nodes are found near the breast in the axilla (under the arm), above the collarbone, and in the chest.
[0005] Breast tumors can be either benign or malignant. Benign tumors are not cancerous, they do not spread to other parts of the body, and are not a threat to life. They can usually be removed, and in most cases, do not come back. Malignant tumors are cancerous, and can invade and damage nearby tissues and organs. Malignant tumor cells may metastisize, entering the bloodstream or lymphatic system. When breast cancer cells metastisize outside the breast, they are often found in the lymph nodes under the arm (axillary lymph nodes). If the cancer has reached these nodes, it means that cancer cells may have spread to other lymph nodes or other organs, such as bones, liver, or lungs.
[0006] Major and intensive research has been focussed on early detection, treatment and prevention. This has included an emphasis on determining the presence of precancerous or cancerous ductal epithelial cells. These cells are analyzed, for example, for cell morphology, for protein markers, for nucleic acid markers, for chromosomal abnormalities, for biochemical markers, and for other characteristic changes that would signal the presence of cancerous or precancerous cells. This has led to various molecular alterations that have been reported in breast cancer, few of which have been well characterized in human clinical breast specimens. Molecular alterations include presence/absence of estrogen and progesterone steroid receptors, HER-2 expression/amplification (Mark H F, et al. HER-2/neu gene amplification in stages I-IV breast cancer detected by fluorescent in situ hybridization. Genet Med; 1(3):98-103 1999), Ki-67 (an antigen that is present in all stages of the cell cycle except G0 and used as a marker for tumor cell proliferation, and prognostic markers (including oncogenes, tumor suppressor genes, and angiogenesis markers) like p53, p27, Cathepsin D, pS2, multi-drug resistance (MDR) gene, and CD31.
[0007] Examination of cells by a trained pathologist has also been used to establish whether ductal epithelial cells are normal (i.e. not precancerous or cancerous or having another noncancerous abnormality), precancerous (i.e. comprising hyperplasia, atypical ductal hyperplasia (ADH)) or cancerous (comprising ductal carcinoma in situ, or DCIS, which includes low grade ductal carcinoma in situ, or LG-DCIS, and high grade ductal carcinoma in situ, or HG-DCIS) or invasive (ductal) carcinoma (IDC). Pathologists may also identify the occurrence of lobular carcinoma in situ (LCIS) or invasive lobular carcinoma (ILC). Breast cancer progression may be viewed as the occurrence of abnormal cells, such as those of ADH, DCIS, IDC, LCIS, and/or ILC, among normal cells.
[0008] It remains unclear whether normal cells become hyperplastic (such as ADH) and then progressing on to become malignant (DCIS, IDC, LCIS, and/or ILC) or whether normal cells are able to directly become malignant without transitioning through a hyperplastic stage. It has been observed, however, that the presence of ADH indicates a higher likelihood of developing a malignancy. This has resulting in treatment of patients with ADH to begin treatment with an antineoplastic/antitumor agent such as tamoxifen. This is in contrast to the treatment of patients with malignant breast cancer which usually includes surgical removal.
[0009] The rational development of preventive, diagnostic and therapeutic strategies for women at risk for breast cancer would be aided by a molecular map of the tumorigenesis process. Relatively little is known of the molecular events that mediate the transition of normal breast cells to the various stages of breast cancer progression. In particular, there is a significant paucity of information regarding the genetic changes that are associated with the earliest stages of human breast cancer, which include the transition of normal breast cells to atypical hyperplastic and/or pre-invasive malignant cells (carcinoma in situ).
[0010] Molecular means of identifying the differences between normal, non-cancerous cells and cancerous cells (in general) have also been the focus of intense study. The use of cDNA libraries to analyze differences in gene expression patterns in normal versus tumorigenic cells has been described (U.S. Pat. No. 4,981,783). DeRisi et al. (1996) describe the analysis of gene expression patterns between two cell lines: UACC-903, which is a tumorigenic human melanoma cell line, and UACC-903(+6), which is a chromosome 6 suppressed non-tumorigenic form of UACC-903. Labeled cDNA probes made from mRNA from these cell lines were applied to DNA microarrays containing 870 different cDNAs and controls. Genes that were preferentially expressed in one of the two cell lines were identified.
[0011] Golub et al. (1999) describe the use of gene expression monitoring as means to cancer class discovery and class prediction between acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). Their approach to class predictors used a neighborhood analysis followed by cross-validation of the validity of the predictors by withholding one sample and building a predictor based only on the remaining samples. This predictor is then used to predict the class of the withheld sample. They also used cluster analysis to identify new classes (or subtypes) within the AML-and ALL.
[0012] Gene expression patterns in human breast cancers have been described by Perou et al. (1999), who studied gene expression between cultured human mammary epithelia cells (HMEC) and breast tissue samples by use of microarrays comprising about 5000 genes. They used a clustering algorithm to identify patterns of expression in HMEC and tissue samples. Perou et al. (2000) describe the use of clustered gene expression profiles to classify subtypes of human breast tumors. Hedenfalk et al. describe gene expression profiles in BRCA1 mutation positive, BRCA2 mutation positive, and sporadic tumors. Sgroi et al. also analyzed gene expression of normal and breast cancer cells from a single patient. Using gene expression patterns to distinguish breast tumor subclasses and predict clinical implications is described by Sorlie et al. and West et al.
[0013] All of the above described approaches, however, utilize heterogeneous populations of cells found in culture or in a biopsy to obtain information on gene expression patterns. The use of such populations may result in the inclusion or exclusion of multiple genes from the patterns. For this and the lack of statistical robustness reasons, the gene expression patterns observed by the above described approaches provide little confidence that the differences in gene expression may be meaningfully associated with the stages of breast cancer.
SUMMARY OF THE INVENTION
[0014] The present invention relates to the identification and use of gene expression patterns (or profiles or “signatures”) which are correlated with (and thus able to discriminate between) cells in various stages of breast cancer. Broadly defined, these stages are non-malignant versus malignant, but may also be viewed as normal versus atypical (optionally including reactive and pre-neoplastic) versus cancerous. Another definition of the stages is normal versus precancerous (e.g. a typical ductal hyperplasia (ADH) or atypical lobular hyperplasia (ALH)) versus cancerous (e.g. carcinoma in situ such as DCIS and/or LCIS) versus invasive (e.g. carcinomas such as IDC and/or ILC). DCIS may be further viewed as low grade versus high grade or grade I through grade III.
[0015] The gene expression patterns comprise one or more than one gene capable of discriminating between various stages of breast cancer with significant accuracy. The gene(s) are identified as correlated with various stages of breast cancer such that the levels of their expression are relevant to a determination of the stage of breast cancer of a cell. Thus in one aspect, the invention provides a method to determine the stage of breast cancer of a subject afflicted with, or suspected of having, breast cancer by assaying a cell containing sample from said subject for expression of one or more than one gene disclosed herein as correlated with one or more stages of breast cancer.
[0016] Gene expression patterns of the invention are identified by analysis of gene expression in multiple samples of each stage to be studied. The overall gene expression profile of each sample is obtained by analyzing the expressed or unexpressed state of genes in each stage relative to each other (one gene to another across all genes). This overall profile is then analyzed to identify genes that are positively, or negatively, correlated, with a stage of breast cancer relative to other genes. An expression profile of a subset of human genes may then be identified by the methods of the present invention as correlated with breast cancer. The use of multiple samples increases the confidence which which a gene may be believed to be correlated with a particular stage. Without sufficient confidence, it remains unpredictable whether a particular gene is actually correlated with a stage of breast cancer and also unpredictable whether a particular gene may be successfully used to identify the stage of an unknown breast cancer cell sample.
[0017] A profile of genes that are highly correlated with one stage relative to another may be used to assay an sample from a subject afflicted with, or suspected of having, breast cancer to identify the stage of breast cancer to which the sample belongs. Such an assay may be used as part of a method to determine the therapeutic treatment for said subject based upon the stage(s) of breast cancer identified.
[0018] The correlated genes may be used singly with significant accuracy or in combination to increase the ability to accurately discriminate between various stages of breast cancer. The present invention thus provides means for correlating a molecular expression phenotype with a physiological (cellular) stage or state. This correlation provides a way to molecularly diagnose and/or monitor a cell's status in comparison to different cancerous versus non-cancerous phenotypes as disclosed herein. Additional uses of the correlated gene(s) are in the classification of cells and tissues; determination of diagnosis and/or prognosis; and determination and/or alteration of therapy.
[0019] The ability to discriminate is conferred by the identification of expression of the individual genes as relevant and not by the form of the assay used to determine the actual level of expression. An assay may utilize any identifying feature of an identified individual gene as disclosed herein as long as the assay reflects, quantitatively or qualitatively, expression of the gene. Identifying features include, but are not limited to, unique nucleic acid sequences used to encode (DNA), or express (RNA), said gene or epitopes specific to, or activities of, a protein encoded by said gene. All that is required is the identity of the gene(s) necessary to discriminate between stages of breast cancer and an appropriate cell containing sample for use in an expression assay.
[0020] In one aspect, the invention provides for the identification of the gene expression patterns by analyzing global, or near global, gene expression from single cells or homogenous cell populations which have been dissected away from, or otherwise isolated or purified from, contaminating cells beyond that possible by a simple biopsy. Because the expression of numerous genes fluctuate between cells from different patients as well as between cells from the same patient sample, multiple individual gene expression patterns are used as reference data to generate models which in turn permit the identification of individual gene(s) that are most highly correlated with particular breast cancer stages and/or have the best the ability to discriminate cells of one stage from another.
[0021] In another aspect, the invention provides physical and methodological means for detecting the expression of gene(s) identified by the models generated by individual expression patterns. These means may be directed to assaying one or more aspect of the DNA template(s) underlying the expression of the gene(s), of the RNA used as an intermediate to express the gene(s), or of the proteinaceous product expressed by the gene(s).
[0022] In a further aspect, the gene(s) identified by a model as capable of discriminating between breast cancer stages may be used to identify the cellular state of an unknown sample of cell(s) from the breast. Preferably, the sample is isolated via non-invasive means. The expression of said gene(s) in said unknown sample may be determined and compared to the expression of said gene(s) in reference data of gene expression patterns from the various stages of breast cancer. Optionally, the comparison to reference samples may be by comparison to the model(s) constructed based on the reference samples.
[0023] One advantage provided by the present invention is that contaminating, non-breast cells (such as infiltrating lymphocytes or other immune system cells) are not present to possibly affect the genes identified or the subsequent analysis of gene expression to identify the status of suspected breast cancer cells. Such contamination is present where a biopsy is used to generate gene expression profiles.
[0024] While the present invention has been described mainly in the context of human breast cancer, it may be practiced in the context of breast cancer of any animal known to be potentially afflicted by breast cancer. Preferred animals for the application of the present invention are mammals, particularly those important to agricultural applications (such as, but not limited to, cattle, sheep, horses, and other “farm animals”) and for human companionship (such as, but not limited to, dogs and cats).
BRIEF DESCRIPTION OF THE FIGURES
[0025]
FIG. 1 is a schematic representing a data matrix of a pair-wise comparison between Grade I and Grade III DCIS among 16 samples (across the top) and a large number of genes identified by “CloneID”) along the left hand side.
[0026]
FIG. 2 is a table showing the actual weight data corresponding to Example II, where the data from ten genes (by CloneID number vertically) are compared to DCIS and ADH samples (across the top). Some data in the table has been vertically presented to permit the table to be displayed on a single sheet. The use of “-” with data in the table reflects genes that are more highly expressed in ADH relative to DCIS. The absence of “-” reflects genes that are more highly expressed in DCIS relative to ADH.
[0027]
FIG. 3 is a table showing the actual weight data corresponding to Example VII, where the data from over 300 genes (by CloneID number vertically) are compared to DCIS and ADH samples (across the top). Some data in the table has been vertically presented solely for display purposes. The use of “-” with data in the table reflects genes that are more highly expressed in ADH relative to DCIS. The absence of “-” reflects genes that are more highly expressed in DCIS relative to ADH.
[0028]
FIG. 4 is a table showing the actual weight data corresponding to Example VIII, where the data from over 300 genes (by CloneID number vertically) are compared to samples (across the top) from two grades of DCIS. The use of “-” with data in the table reflects genes that are more highly expressed in grade I relative to grade III. The absence of “-” reflects genes that are more highly expressed in grade III relative to grade I.
DETAILED DESCRIPTION OF THE SPECIFIC EMBODIMENTS
[0029] Definitions of Terms as Used Herein:
[0030] A gene expression “pattern” or “profile” or “signature” refers to the relative expression of a gene between two or more stages of breast cancer which is correlated with being able to distinguish between said stages.
[0031] A “gene” is a polynucleotide that encodes a discrete product, whether RNA or proteinaceous in nature. It is appreciated that more than one polynucleotide may be capable of encoding a discrete product. The term includes alleles and polymorphisms of a gene that encodes the same product, or a functionally associated (including gain, loss, or modulation of function) analog thereof, based upon chromosomal location and ability to recombine during normal mitosis.
[0032] A “stage” or “stages” (or equivalents thereof) of breast cancer refer to a physiologic state of a breast cell as defined by known cytological or histological (including immunohistology, histochemistry, and immunohistochemistry) procedures and are readily known to skilled in the art. Non-limiting examples include normal versus abnormal, non-cancerous versus cancerous, the different stages described herein (e.g. hyperplastic, carcinoma, and invasive), and grades within different stages (e.g. grades I, II, or III or the equivalents thereof within cancerous stages).
[0033] The terms “correlate” or “correlation” or equivalents thereof refer to an association between expression of one or more genes and a physiologic state of a breast cell to the exclusion of one or more other stages and/or identified by use of the methods as described herein. A gene may be expressed at higher or lower levels and still be correlated with one or more breast cancer stages.
[0034] A “polynucleotide” is a polymeric form of nucleotides of any length, either ribonucleotides or deoxyribonucleotides. This term refers only to the primary structure of the molecule. Thus, this term includes double- and single-stranded DNA and RNA. It also includes known types of modifications including labels known in the art, methylation, “caps”, substitution of one or more of the naturally occurring nucleotides with an analog, and internucleotide modifications such as uncharged linkages (e.g., phosphorothioates, phosphorodithioates, etc.), as well as unmodified forms of the polynucleotide.
[0035] The term “amplify” is used in the broad sense to mean creating an amplification product can be made enzymatically with DNA or RNA polymerases. “Amplification,” as used herein, generally refers to the process of producing multiple copies of a desired sequence, particularly those of a sample. “Multiple copies” mean at least 2 copies. A “copy” does not necessarily mean perfect sequence complementarity or identity to the template sequence.
[0036] By corresponding is meant that a nucleic acid molecule shares a substantial amount of sequence identity with another nucleic acid molecule. Substantial amount means at least 95%, usually at least 98% and more usually at least 99%, and sequence identity is determined using the BLAST algorithm, as described in Altschul et al. (1990), J. Mol. Biol. 215:403-410 (using the published default setting, i.e. parameters w=4, t=17). Methods for amplifying mRNA are generally known in the art, and include reverse transcription PCR (RT-PCR) and those described in U.S. patent application (number to be assigned) entitled “Nucleic Acid Amplification” filed on Oct. 25, 2001 as attorney docket number 485772002900 as well as U.S. Provisional Patent Applications 60/298,847 (filed Jun. 15, 2001) and 60/257,801 (filed Dec. 22, 2000), all of which are hereby incorporated by reference in their entireties as if fully set forth. Alternatively, RNA may be directly labeled as the corresponding cDNA by methods known in the art.
[0037] A “microarray” is a linear or two-dimensional array of preferably discrete regions, each having a defined area, formed on the surface of a solid support such as, but not limited to, glass, plastic, or synthetic membrane. The density of the discrete regions on a microarray is determined by the total numbers of immobilized polynucleotides to be detected on the surface of a single solid phase support, preferably at least about 50/cm2, more preferably at least about 100/cm2, even more preferably at least about 500/cm2, but preferably below about 1,000/cm2. Preferably, the arrays contain less than about 500, about 1000, about 1500, about 2000, about 2500, or about 3000 immobilized polynucleotides in total. As used herein, a DNA microarray is an array of oligonucleotides or polynucleotides placed on a chip or other surfaces used to hybridize to amplified or cloned polynucleotides from a sample. Since the position of each particular group of primers in the array is known, the identities of a sample polynucleotides can be determined based on their binding to a particular position in the microarray.
[0038] Because the invention relies upon the identification of genes that are over- or under-expressed, one embodiment of the invention involves determining expression by hybridization of mRNA, or an amplified or cloned version thereof, of a sample cell to a polynucleotide that is unique to a particular gene sequence. Preferred polynucleotides of this type contain at least about 20, at least about 22, at least about 24, at least about 26, at least about 28, at least about 30, or at least about 32 consecutive basepairs of a gene sequence that is not found in other gene sequences. The term “about” as used in the previous sentence refers to an increase or decrease of 1 from the stated numerical value. Even more preferred are polynucleotides of at least about 50, at least about 100, and at least about 150 basepairs of a gene sequence that is not found in other gene sequences. The term “about” as used in the preceding sentence refers to an increase or decrease of 10% from the stated numerical value.
[0039] Alternatively, and in another embodiment of the invention, gene expression may be determined by analysis of expressed protein in a cell sample of interest by use of one or more antibodies specific for one or more epitopes of individual gene products (proteins) in said cell sample. Such antibodies are preferably labeled to permit their easy detection after binding to the gene product.
[0040] The term “label” refers to a composition capable of producing a detectable signal indicative of the presence of the labeled molecule. Suitable labels include radioisotopes, nucleotide chromophores, enzymes, substrates, fluorescent molecules, chemiluminescent moieties, magnetic particles, bioluminescent moieties, and the like. As such, a label is any composition detectable by spectroscopic, photochemical, biochemical, immunochemical, electrical, optical or chemical means.
[0041] The term “support” refers to conventional supports such as beads, particles, dipsticks, fibers, filters, membranes and silane or silicate supports such as glass slides.
[0042] As used herein, a “breast tissue sample” or “breast cell sample” refers to a sample of breast tissue or fluid isolated from an individual suspected of being afflicted with, or at risk of developing, breast cancer. Such samples are primary isolates (in contrast to cultured cells) and may be collected by any non-invasive means, including, but not limited to, ductal lavage, fine needle aspiration, needle biopsy, the devices and methods described in U.S. Pat. No. 6,328,709, or any other suitable means recognized in the art. Alternatively, the “sample” may be collected by an invasive method, including, but not limited to, surgical biopsy.
[0043] “Expression” and “gene expression” include transcription and/or translation of nucleic acid material.
[0044] As used herein, the term “comprising” and its cognates are used in their inclusive sense; that is, equivalent to the term “including” and its corresponding cognates.
[0045] Conditions that “allow” an event to occur or conditions that are “suitable” for an event to occur, such as hybridization, strand extension, and the like, or “suitable” conditions are conditions that do not prevent such events from occurring. Thus, these conditions permit, enhance, facilitate, and/or are conducive to the event. Such conditions, known in the art and described herein, depend upon, for example, the nature of the nucleotide sequence, temperature, and buffer conditions. These conditions also depend on what event is desired, such as hybridization, cleavage, strand extension or transcription.
[0046] Sequence “mutation,” as used herein, refers to any sequence alteration in the sequence of a gene disclosed herein interest in comparison to a reference sequence. A sequence mutation includes single nucleotide changes, or alterations of more than one nucleotide in a sequence, due to mechanisms such as substitution, deletion or insertion. Single nucleotide polymorphism (SNP) is also a sequence mutation as used herein. Because the present invention is based on the relative level of gene expression, mutations in non-coding regions of genes as disclosed herein may also be assayed in the practice of the invention.
[0047] “Detection” includes any means of detecting, including direct and indirect detection of gene expression and changes therein. For example, “detectably less” products may be observed directly or indirectly, and the term indicates any reduction (including the absence of detectable signal). Similarly, “detectably more” product means any increase, whether observed directly or indirectly.
[0048] Unless defined otherwise all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this invention belongs.
[0049] Specific Embodiments
[0050] The present invention relates to the identification and use of gene expression patterns (or profiles or “signatures”) which discriminate between (or are correlated with) cells in various stages of breast cancer. Such patterns may be determined by the methods of the invention by use of a number of reference cell or tissue samples, such as those reviewed by a pathologist of ordinary skill in the pathology of breast cancer, which reflect various stages of breast cancer. Because the overall gene expression profile differs from person to person, cancer to cancer, and cancer cell to cancer cell, correlations between certain cell states and genes expressed or underexpressed may be made as disclosed herein to identify genes that are capable of discriminating between different breast cancer states.
[0051] The present invention may be practiced with any number of genes believed, or likely to be, differentially expressed in breast cancer cells. In Example I below, approximately 12,000 genes were used to identify hundreds of genes capable of discriminating between various stages of breast cancer as shown in Examples 2-9. The identification may be made by using expression profiles of various homogenous normal and breast cancer cell populations, which were isolated by microdissection, such as, but not limited to, laser capture microdissection (LCM) of 100-1000 cells. Each gene of the expression profile may be assigned weights based on its ability to discriminate between two or more stages of breast cancer (see Example I). The magnitude of each assigned weight indicates the extent of difference in expression between the two groups and is an approximation of the ability of expression of the gene to discriminate between the two groups (and thus stages). The magnitude of each assigned weight also approximates the extent of correlation between expression of individual gene(s) and particular breast cancer stages.
[0052] It should be noted that merely high levels of expression in cells from a particular stage or stages does not necessarily mean that a gene will be identified as having a high absolute weight value.
[0053] Genes with top ranking weights (in absolute terms) may be used to generate models of gene expressions that would maximally discriminate between the two groups. Alternatively, genes with top ranking weights (in absolute terms) may be used in combination with genes with lower weights without signficant loss of ability to discriminate between groups. Such models may be generated by any appropriate means recognized in the art, including, but not limited to, cluster analysis, supported vector machines, neural networks or other algorithm known in the art. The models are capable of predicting the classification of a unknown sample based upon the expression of the genes used for discrimination in the models. “Leave one out” cross-validation may be used to test the performance of various models and to help identify weights (genes) that are uninformative or detrimental to the predictive ability of the models. Cross-validation may also be used to identify genes that enhance the predictive ability of the models.
[0054] The gene(s) identified as correlated with particular breast cancer stages by the above models provide the ability to focus gene expression analysis to only those genes that contribute to the ability to identify a cell as being in a particular stage of breast cancer relative to another stage or stages. The expression of other genes in a breast cancer cell would be relatively unable to provide information concerning, and thus assist in the discrimination of, different stages of breast cancer. For example, the alpha subunit of human topoisomerase II (identified by CloneID 825470) has been found to be useful in discriminations between normal and atypical cells (ADH and DCIS and IDC and LCIS), between normal and ADH cells compared to DCIS and IDC cells, between normal and DCIS cells, between ADH and DCIS cells, between grade I and grade III DCIS cells, and between grade I and grade III IDC cells but not between normal and ADH cells (see Examples II to IX below). Thus expression of this topoisomerase II subunit would be utilized in models to discriminate between the above listed stages but not for discerning normal from ADH cells. This type of analysis is readily incorporated into algorithms used to generate models with reference gene expression data.
[0055] As will be appreciated by those skilled in the art, the models are highly useful with even a small set of reference gene expression data and can become increasingly accurate with the inclusion of more reference data although the incremental increase in accuracy will likely diminish with each additional datum. The preparation of additional reference gene expression data using genes identified and disclosed herein for discriminating between different stages of breast cancer is routine and may be readily performed by the skilled artisan to permit the generation of models as described above to predict the status of an unknown sample based upon the expression levels of those genes.
[0056] To determine the expression levels of genes in the practice of the present invention, any method known in the art may be utilized. In one preferred embodiment of the invention, expression based on detection of RNA which hybridizes to the genes identified and disclosed herein is used. This is readily performed by any RNA detection or amplification+detection method known or recognized as equivalent in the art such as, but not limited to, reverse transcription-PCR, the methods disclosed in U.S. patent application (number to be assigned) entitled “Nucleic Acid Amplification” filed on Oct. 25, 2001 as attorney docket number 485772002900 as well as U.S. Provisional Patent Applications 60/298,847 (filed Jun. 15, 2001) and 60/257,801 (filed Dec. 22, 2000), and methods to detect the presence, or absence, of RNA stabilizing or destabilizing sequences.
[0057] Alternatively, expression based on detection of DNA status may be used. Detection of the DNA of an indentified gene as methylated or deleted may be used for genes that have decreased expression in correlation with a particular breast cancer stage. This may be readily performed by PCR based methods known in the art. Conversely, detection of the DNA of an indentified gene as amplified may be used for genes that have increased expression in correlation with a particular breast cancer stage. This may be readily performed by PCR based, fluorescent in situ hybridization (FISH) and chromosome in situ hybridization (CISH) methods known in the art.
[0058] Expression based on detection of a presence, increase, or decrease in protein levels or activity may also be used. Detection may be performed by any immunohistochemistry (IHC) based, blood based (especially for secreted proteins), antibody (including autoantibodies against the protein) based, ex foliate cell (from the cancer) based, mass spectroscopy based, and image (including used of labeled ligand) based method known in the art and recognized as appropriate for the detection of the protein. Antibody and image based methods are additionally useful for the localization of tumors after determination of cancer by use of cells obtained by a non-invasive procedure (such as ductal lavage or fine needle aspiration), where the source of the cancerous cells is not known. A labeled antibody or ligand may be used to localize the carcinoma(s) within a patient.
[0059] A preferred embodiment using a nucleic acid based assay to determine expression is by immobilization of one or more of the genes identified herein on a solid support, including, but not limited to, a solid substrate as an array or to beads or bead based technology as known in the art. Alternatively, solution based expression assays known in the art may also be used. The immobilized gene(s) may be in the form of polynucleotides that are unique or otherwise specific to the gene(s) such that the polynucleotide would be capable of hybridizing to a DNA or RNA corresponding to the gene(s). These polynucleotides may be the full length of the gene(s) or be short sequences of the genes that are optionally minimally interrupted (such as by mismatches or inserted non-complementary basespairs) such that hybridization with a DNA or RNA corresponding to the gene(s) is not affected.
[0060] The immobilized gene(s) may be used to determine the state of nucleic acid samples prepared from sample breast cell(s) for which the pre-cancer or cancer status is not known or for confirmation of a status that is already assigned to the sample breast cell(s). Without limiting the invention, such a cell may be from a patient suspected of being afflicted with, or at risk of developing, breast cancer. The immobilized polynucleotide(s) need only be sufficient to specifically hybridize to the corresponding nucleic acid molecules derived from the sample. While even a single correlated gene sequence may to able to provide adequate accuracy in discriminating between two breast cancer cell stages, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more of the genes identified herein may be used as a subset capable of discriminating may be used in combination to increase the accuracy of the method. The invention specifically contemplates the selection of more than one, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more of the genes disclosed in the tables and figures herein for use as a subset in the identification of whether an unknown or suspicious breast cancer sample is normal or is in one or more stages of breast cancer. Optionally, the genes used will not include CloneID 809507, which is also known as GenBank accession number AA454563, described as an EST with high similarity to CD63 but of unknown function.
[0061] In embodiments where only one or a few genes are to be analyzed, the nucleic acid derived from the sample breast cancer cell(s) may be preferentially amplified by use of appropriate primers such that only the genes to be analyzed are amplified to reduce contaminating background signals from other genes expressed in the breast cell. Alternatively, and where multiple genes are to be analyzed or where very few cells (or one cell) is used, the nucleic acid from the sample may be globally amplified before hybridization to the immobilized polynucleotides. Of course RNA, or the cDNA counterpart thereof may be directly labeled and used, without amplification, by methods known in the art.
[0062] The above assay embodiments may be used in a number of different ways to identify or detect the breast cancer stage, if any, of a breast cancer cell sample from a patient. In many cases, this would reflect a secondary screen for the patient, who may have already undergone mammography or physical exam as a primary screen. If positive, the subsequent needle biopsy, ductal lavage, fine needle aspiration, or other analogous methods may provide the sample for use in the above assay embodiments. The present invention is particularly useful in combination with non-invasive protocols, such as ductal lavage or fine needle aspiration, to prepare a breast cell sample. The current analysis of ductal lavage samples is by cytological examination by a trained pathologist who classifies the samples in terms that are at least partly subjective: unsatisfactory (too few cells), benign (including fibrocystic change), atypical (or mild atypia), suspicious (or marked atypia), or malignant.
[0063] The present invention provides a more objective set of criteria, in the form of gene expression profiles of a discrete set of genes, to discriminate (or delineate) between meaningful stages (or classes) of breast cancer cells. In particularly preferred embodiments of the invention, the assays are used to discriminate between ADH and DCIS or otherwise malignant cells, which is a critical determination for decisions concerning subsequent treatment and therapy for the patient. Another particularly preferred determination is between the three grades (I, II, III) of carcinomas in situ as well as the discrimination between grade III carcinomas in situ and invasive carcinomas. Other pairwise comparisons that are provided by the invention include, but are not limited to, normal versus ADH, normal versus carcinoma in situ, normal versus invasive, normal versus cancerous (i.e. carcinoma present), ADH versus cancerous, and carcinoma in situ versus invasive. With the use of alternative algorithms, such as neural networks, comparisons that discriminate between multiple (more than pairwise) classes may also be performed. It is believed by the inventors that the present invention is the first example of objective, molecular criteria for making these discriminations.
[0064] In one embodiment of the invention, the isolation and analysis of a breast cancer cell sample may be performed as follows:
[0065] (1) Ductal lavage or other non-invasive procedure is performed on a patient to obtain a sample.
[0066] (2) Sample is prepared and coated onto a microscope slide. Note that ductal lavage results in clusters of cells that are cytologically examined as stated above.
[0067] (3) Pathologist or image analysis software scans the sample for the presence of atypical cells.
[0068] (4) If atypical cells are observed, those cells are harvested (e.g. by microdissection such as LCM).
[0069] (5) RNA is extracted from the harvested cells.
[0070] (6) RNA is purified, amplified, and labeled.
[0071] (7) Labeled nucleic acid is contacted with a microarray containing polynucleotides of the genes identified herein as correlated to discriminations between two or more stages of breast cancer under hybridization conditions, then processed and scanned to obtain a pattern of intensities of each spot (relative to a control for general gene expression in cells) which determine the level of expression of the gene(s) in the cells.
[0072] (8) The pattern of intensities is analyzed by comparison to the expression patterns of the genes in known samples of normal and breast cancer cells (relative to the same control).
[0073] A specific example of the above method would be performing ductal lavage following a primary screen, observing and collecting atypical cells for analysis. The comparison to known expression patterns, such as that made possible by a model generated by an algorithm (such as, but not limited to nearest neighbor type analysis, SVM, or neural networks) with reference gene expression data for the different breast cancer stages, identifies the cells as being most likely ADH.
[0074] Alternatively, the sample may permit the collection of both normal and atypical cells for analysis. The gene expression patterns for each of these two samples will be compared to each other as well as the model and the normal versus individual abnormal comparisons therein based upon the reference data set. This approach can be significantly more powerful that the atypical cells only approach because it utilizes significantly more information from the normal cells and the differences between normal and atypical cells (in both the sample and reference data sets) to determine the status of the atypical cells from the sample.
[0075] By appropriate selection of the genes used in the analysis, identification of the relative amounts of atypical cells may also be possible, although in most clinical settings, the identification of the highest grade of breast cancer with confidence makes identification of lower grades less important. Stated differently, the identification of invasive cancer determines the clinical situation regardless of the presence of carcinoma in situ or hyperplastic cells, or the identification of carcinoma in situ makes determines the clinical situation regardless of the presence of hyperplastic cells.
[0076] With use of the present invention, skilled physicians may prescribe treatments based on non-invasive samples that they would have prescribed for a patient which had previously received a diagnosis via a solid tissue biopsy.
[0077] The above discussion is also applicable where a palpable lesion is detected followed by fine needle aspiration or needle biopsy of cells from the breast. The cells are plated and reviewed by a pathologist or automated imaging system which selects cells for analysis as described above. This again provides a means of linking visual to molecular cytology and provides a less subjective means of identifying the physiological state of breast cancer cells without the need for invasive solid tissue biopsies.
[0078] The present invention may also be used, however, with solid tissue biopsies. For example, a solid biopsy may be collected and prepared for visualization followed by determination of expression of one or more genes identified herein to determine the stage of breast cancer, if any. One preferred means is by use of in situ hybridization with polynucleotide or protein identifying probe(s) for assaying expression of said gene(s).
[0079] In an alternative method, the solid tissue biopsy may be used to extract molecules followed by analysis for expression of one or more gene(s). This provides the possibility of leaving out the need for visualization and collection of only those cells suspected of being atypical. This method may of course be modified such that only cells suspected of being atypical are collected and used to extract molecules for analysis. This would require visualization and selection as an prerequisite to gene expression analysis.
[0080] In a further modification of the above, both normal cells and cells suspected of being atypical are collected and used to extract molecules for analysis of gene expression. The approach, benefits and results are as described above using non-invasive sampling.
[0081] In a further alternative to all of the above, the gene(s) identified herein may be used as part of a simple PCR or array based assay simply to determine the presence of atypical cells in a sample from a non-invasive sampling procedure. This is simple to perform and utilizes genes identified to be the best discriminators of normal versus abnormal cells without the need for any cytological examination. If no atypical cells are identified, no cytological examination is necessary. If atypical cells are identified, cytological examination follows, and a more comprehensive analysis, as described above, may follow.
[0082] The genes identified herein may be used to generate a model capable of predicting the breast cancer stage (if any) of an unknown breast cell sample based on the expression of the identified genes in the sample. Such a model may be generated by any of the algorithms described herein or otherwise known in the art as well as those recognized as equivalent in the art using gene(s) (and subsets thereof) disclosed herein for the identification of whether an unknown or suspicious breast cancer sample is normal or is in one or more stages of breast cancer. The model provides a means for comparing expression profiles of gene(s) of the subset from the sample against the profiles of reference data used to build the model. The model can compare the sample profile against each of the reference profiles or against model defining delineations made based upon the reference profiles. Additionally, relative values from the sample profile may be used in comparison with the model or reference profiles.
[0083] In a preferred embodiment of the invention, breast cell samples identified as normal and abnormal (atypical) from the same subject may be analyzed for their expression profiles of the genes used to generate the model. This provides an advantageous means of identifying the stage of the abnormal sample based on relative differences from the expression profile of the normal sample. These differences can then be used in comparison to differences between normal and individual abnormal reference data which was also used to generate the model.
[0084] The detection of gene expression from the samples may be by use of a single microarray able to assay gene expression from all pairwise comparisons disclosed herein for convenience and accuracy.
[0085] Other uses of the present invention include providing the ability to identify breast cancer cell samples as being those of a particular stage of cancer for further research or study. This provides a particular advantage in many contexts requiring the identification of breast cancer stage based on objective genetic or molecular criteria rather than cytological observation. It is of particular utility to distinguish different grades of a particular breast cancer stage for further study, research or characterization because no objective criteria for such delineation was previously available.
[0086] The materials for use in the methods of the present invention are ideally suited for preparation of kits produced in accordance with well known procedures. The invention thus provides kits comprising agents for the detection of expression of the disclosed genes for identifying breast cancer stage. Such kits optionally comprising the agent with an identifying description or label or instructions relating to their use in the methods of the present invention, is provided. Such a kit may comprise containers, each with one or more of the various reagents (typically in concentrated form) utilized in the methods, including, for example, prefabricated microarrays, buffers, the appropriate nucleotide triphosphates (e.g., dATP, dCTP, dGTP and dTTP; or rATP, rCTP, rGTP and UTP), reverse transcriptase, DNA polymerase, RNA polymerase, and one or more primer complexes of the present invention (e.g., appropriate length poly(T) or random primers linked to a promoter reactive with the RNA polymerase). A set of instructions will also typically be included.
[0087] The methods provided by the present invention may also be automated in whole or in part. All aspects of the present invention may also be practiced such that they consist essentially of a subset of the disclosed genes to the exclusion of material irrelevant to the identification of breast cancer stages in a cell containing sample.
[0088] Having now generally described the invention, the same will be more readily understood through reference to the following examples which are provided by way of illustration, and are not intended to be limiting of the present invention, unless specified.
Example I
[0089] Materials and Methods
[0090] Clinical Specimens
[0091] Clinical biopsies from 30 patients were obtained from the Massachusetts General Hospital with Institutional Review Board approval. The tissue from one of the patients was not associated with breast cancer of any kind since it was from a breast reduction procedure. Pathological and histological information for the biopsies were also obtained. Three independent captures of about 1000 breast epithelial cells of one or more of the four different disease stages (normal, N; atypical ductal hyperplasia, ADH; ductal carcinoma in situ, DCIS; invasive ductal carcinoma, IDC) were procured from each biopsy using Laser Capture Microdissection (LCM, Arcturus Engineering). Three independent captures of LCIS (lobular carcinoma in situ) in one biopsy were also made. Total RNA was extracted from the captured (procured) cells and amplified with a T7-promoter based RNA amplification protocol. The human universal reference RNA (Stratagene, La Jolla), was similarly amplified and used as the reference channel in a two-color microarray hybridization.
[0092] Micro Arrays
[0093] To maximize coverage of breast cancer-related genes on the microarrays used, 11,435 cDNA clones from the IMAGE consortium (Research Genetics) were obtained. These clones were selected based on literature knowledge (such as, but not limited to, preferential expression in cancer versus normal cells) and after mining (such as, but not limited to, preferential expression in breast tissues) gene expression information in the expressed sequence tags (EST) databases and the Serial Analysis of Gene Expression (SAGE) data sets available from the National Center for Biotechnology Information (NCBI, http://www/ncbi.nlm.nih.gov).
[0094] Microarray Data Processing
[0095] Microarray images were analyzed with InaGene (BioDiscovery) to find and quantitate each spot on the microarray. Spots flagged by ImaGene as poor spots using standard criteria used with the software for the standardization of signals were excluded from further analysis. Raw Cy5 (sample channel) and Cy3 (reference channel) intensities and associated local background estimates for each spot were then examined. The signal/noise ratio, defined as the spot intensity over background intensity, was used as the second criteria for spot exclusion; spots with signal/noise ratio <3.0 in the reference channel or <1.5 in the sample channel were excluded from further analysis. Background-subtracted intensities across the chip were normalized to the 75th-percentile of the spot intensity distribution on the entire chip (alternative normalizations to the mean, median or other point may also be used as known in the art). Cy5/Cy3 ratios of each spot for each cellular state were averaged across each of six measurements (3 LCM captures×2 chips/capture=6 chips); outliers among the 6 data points were removed before taking the average. The resulting data were formatted as a data matrix (samples along the top horizontal axis and gene identity along the vertical axis) for data mining (see FIG. 1 with data).
[0096] Microarray Data Analysis
[0097] Before further analysis, each value in a row (gene) of the gene expression matrix was divided by the median value for the row, and the resulting matrix log-transformed. Normalized, median-centered, and log-transformed, gene expression data matrix was loaded into GeneMaths software (Applied-Maths, Belgium). Clustering and discriminant analysis were performed to identify sets of genes associated with different cellular states. For each pair-wise comparison between two breast cancer stages, samples are assigned to either the positive group or negative group, and genes were sorted by their discriminatory weights. The absolute value of the weight of a gene indicates the extent of difference in expression between the two groups; the positively signed genes are expressed higher in one group and the negatively singed genes are expressed higher in the other group.
[0098] The utility of the top-ranking genes as a diagnostic test was evaluated using the support vector machines (SVMs) algorithm (see Yeang, C. H., S. Ramaswamy, et al. (2001). “Molecular classification of multiple tumor types.” Bioinformatics 17 Suppl 1: S316-22; Xiong, M., X. Fang, et al. (2001). “Biomarker identification by feature wrappers.” Genome Res 11(11): 1878-87this one used linear discriminate analysis, logistic regression and svm; Furey, T. S., N. Cristianini, et al. (2000). “Support vector machine classification and validation of cancer tissue samples using microarray expression data.” Bioinformatics 16(10): 906-14; and Brown, M. P., W. N. Grundy, et al. (2000). “Knowledge-based analysis of microarray gene expression data by using support vector machines.” Proc Natl Acad Sci USA 97(1): 262-7, who state “SVMs are considered a supervised computer learning method because they exploit prior knowledge of gene function to identify unknown genes of similar function from expression data. SVMs avoid several problems associated with unsupervised clustering methods, such as hierarchical clustering and self-organizing maps.”) Other algorithms, such as, but not limited to, linear discriminate analysis, logistic regression, cluster analysis, K-th nearest neighbor, or neural nets.
[0099] The support vector machines algorithm finds the maximal margin hyperplane that separate the two groups under comparison. The method of leave-one-out cross-validation was used to test the performance of a given set of genes; one sample was taken in turn out of the training set and a model is built using the rest of the training set, which is in then applied to classify the left-out sample. The accuracy of the genes in the cross-validation procedure is the percentage of correct classifications over the total number of the training samples.
Example II
[0100] 10Genes for Discriminating Between ADH and DCIS
[0101] Based upon the methodology of Example I above, 10 genes identified as capable of discriminating between ADH and DCIS are listed in Table 1 below along with a brief description of the gene. CloneID as used in the context of the present invention refers to the IMAGE Consortium clone ID number of each gene, the sequences of which are hereby incorporated by, reference in their entireties as they are available from the Consortium at http://image.llnl.gov/ as accessed on the filing date of the present application. Weight refers to the absolute value indicating the extent of difference in expression between ADH and DCIS where the positively signed values are expressed higher in ADH and the negatively signed values are expressed higher in DCIS; Chromosome Location refers to the human chromosome to which the gene has been assigned, and Description provides a brief identifier of what the gene encodes. The actual data corresponding to the assigned weights are shown in FIG. 2.
1TABLE 1
|
|
GeneIDWeightChromosome LocationDescription
|
|
8254700.994655517q21-q22 topoisomerase (DNA) II alpha (170 kD)
5952130.98848848hypothetical protein
baculoviral IAP repeat-containing 5
7966940.985268617q25(survivin)
1404774−1.563873812p12.1-p11.2 parathyroid hormone-like hormone
823871−1.52604647SPARC-like 1 (mast9, hevin)
1882697−1.40187817q22-q23 peanut (Drosophila)-like 2
140071−1.38819542frizzled-related protein
ESTs, Weakly similar to 2004399A
160192−1.25516635chromosomal protein [H. sapiens]
796542−1.14018533q28ets variant gene 5 (ets-related molecule)
611532−1.122759711p15.5troponin I, skeletal, fast
|
Example III
[0102] Genes for Discriminating Between Normal and Non-Normal (a Combination of ADH, DCIS, and IDC) Cells from Breast Tissue
[0103] As shown in Table 2 below, 850 genes were identified as being able to discriminate between normal and “abnormal” (defined in this instance as any sample that was not normal), which includes ADH, DCIS and IDC.
2TABLE 2
|
|
CloneIDWeightDescription
|
|
13234481.5470535cysteine-rich protein 1 (intestinal)
7886541.4818381growth factor receptor-bound protein 2
351471.3764654“ESTs, Weakly similar to unnamed protein product
[H. sapiens]”
7456061.3470375hypothetical protein PP591
15000001.3204029“H2B histone family, member B”
5950371.3057353retinoic acid induced 3
5653191.2369212Homo sapiens mRNA; cDNA DKFZp564B1264 (from clone
DKFZp564B1264)
1220771.2356703putative membrane protein
16098361.2349106glutamate-ammonia ligase (glutamine synthase)
15050381.2292506hypothetical protein FLJ20171
1788051.2225746“Human DNA sequence from clone RP5-850E9 on
chromosome 20. Contains part of the gene for a novel
C2H2 type zinc finger protein similar to Drosophila Scratch
(Scrt), Slug and Xenopus Snail, a novel gene similar to
Drosophila CG6762, STSs, GSSs and five CpG
8122381.2132886hypothetical protein MGC4692
14922381.1991653HSPC003 protein
3661321.1911439“succinate dehydrogenase complex, subunit C, integral
membrane protein, 15 kD”
7964691.1850928HSPC150 protein similar to ubiquitin-conjugating enzyme
4889641.1545223“H2A histone family, member O”
4715681.1466811hematological and neurological expressed 1
15545491.1440029hydroxyacyl glutathione hydrolase
2839191.118257“H2A histone family, member L”
14924631.087152“selenoprotein X, 1”
1994031.082569“lectin, galactoside-binding, soluble, 8 (galectin 8)”
7967231.0724133Homo sapiens clone CDABP0014 mRNA sequence
1381891.0692241Wolfram syndrome 1 (wolframin)
15740581.0651351“1-acylglycerol-3-phosphate O-acyltransferase 2
(lysophosphatidic acid acyltransferase, beta)”
8117741.0617336CGI-49 protein
3598871.052326translocase of inner mitochondrial membrane 17 (yeast)
homolog A
17097911.0378948BAI1-associated protein 1
2448011.0162794Rho guanine exchange factor (GEF) 11
19179410.9960315purine-rich element binding protein B
18588920.9897362hypothetical protein MGC4825
2889990.9896012small protein effector 1 of Cdc42
5032150.9757636pilin-like transcription factor
19113430.967716“RAB26, member RAS oncogene family”
20291730.9672743“ESTs, Weakly similar to N-WASP [H. sapiens]”
4700610.9665514seven in absentia (Drosophila) homolog 2
20169080.9615102“ESTs, Weakly similar to CA13_HUMAN COLLAGEN
ALPHA 1(III) CHAIN PRECURSOR [H. sapiens]”
14694250.9610542SRY (sex determining region Y)-box 22
8140540.958989KIAA0040 gene product
8145280.9569471“Homo sapiens cDNA: FLJ22139 fis, clone HEP20959”
686360.9562688hypothetical protein MGC2477
2908410.9562201“H2B histone family, member A”
2360340.9534619“uncoupling protein 2 (mitochondrial, proton carrier)”
7824280.9524489KIAA0250 gene product
1310940.9518372“Homo sapiens cDNA: FLJ21587 fis, clone COL06946”
4882020.9501137“ESTs, Weakly similar to YZ28_HUMAN HYPOTHETICAL
PROTEIN ZAP128 [H. sapiens]”
14358620.9500093“antigen identified by monoclonal antibodies 12E7, F21 and
O13”
8235980.9452282“proteasome (prosome, macropain) 26S subunit, non-
ATPase, 12”
14924260.9397097chromosome 19 open reading frame 3
7951850.9295627xenotropic and polytropic retrovirus receptor
8105580.9227709“proteasome (prosome, macropain) 26S subunit, ATPase,
4”
4696860.9203022“Ric (Drosophila)-like, expressed in many tissues”
2803750.9155003PRO2000 protein
7299750.9149894meningioma expressed antigen 5 (hyaluronidase)
8101240.9124035“platelet-activating factor acetylhydrolase, isoform lb,
gamma subunit (29 kD)”
8132810.9107661WW domain-containing protein 1
398840.9091807IMP (inosine monophosphate) dehydrogenase 1
766050.9028609nesca protein
16360920.8997881hypothetical protein FLJ20657
7414740.8925439glucose phosphate isomerase
16054260.8909868hypothetical protein FLJ13352
7886540.8886269
8681280.8873517JM4 protein
3084660.8803492GTP-binding protein Sara
442920.8732235Homo sapiens mRNA; cDNA DKFZp434C107 (from clone
DKFZp434C107)
8262560.8700831transmembrane 7 superfamily member 1 (upregulated in
kidney)
6855160.8694652putative G protein-coupled receptor
8107110.869424stearoyl-CoA desaturase (delta-9-desaturase)
7532990.8679015hypothetical protein FLJ10504
7310440.8657953glutaredoxin 2
8240520.8650616chromosome 6 open reading frame 1
8431950.86501phosphoserine phosphatase
2927700.8570995“Homo sapiens, clone IMAGE: 3627860, mRNA, partial cds”
8597610.8563964poliovirus receptor-related 2 (herpesvirus entry mediator B)
3473730.8555396“transcription elongation factor B (SIII), polypeptide 1
(15 kD, elongin C)”
16408210.8544081“ESTs, Weakly similar to I78885 serine/threonine-specific
protein kinase [H. sapiens]”
7044140.8520508small nuclear ribonucleoprotein polypeptides B and B1
8107250.8510425“ATPase, H+ transporting, lysosomal (vacuolar proton
pump) 21 kD”
7826080.8507815mitochondrial ribosomal protein L9
20192230.849044mitochondrial ribosomal protein L17
21105110.8471736artemin
14749550.8460414“TATA box binding protein (TBP)-associated factor, RNA
polymerase II, N, 68 kD (RNA-binding protein 56)”
8106120.842284S100 calcium-binding protein A11 (calgizzarin)
7444170.8409538carnitine acetyltransferase
9698770.8382113“synaptosomal-associated protein, 25 kD”
2725290.8371698phosphomannomutase 2
7685700.8371438hypothetical protein FLJ11280
8248790.8366695hypothetical protein MGC11275
20140340.8354357“methylene tetrahydrofolate dehydrogenase (NAD+
dependent), methenyltetrahydrofolate cyclohydrolase”
20546350.831532“proteasome (prosome, macropain) subunit, alpha type, 7”
20521130.8307595hypothetical protein FLJ10903
7425950.8291965cyclin-dependent kinase 5
20166480.8263671Homo sapiens mRNA; cDNA DKFZp434N1728 (from clone
DKFZp434N1728)
15732510.823025peroxisomal long-chain acyl-coA thioesterase
18692010.8202299hypothetical protein MGC2745
377080.8194577hypothetical protein MGC3101
2413480.8182422prenylcysteine lyase
8100630.8168926“growth factor, erv1 (S. cerevisiae)-like (augmenter of liver
regeneration)”
7313080.8166873citrate synthase
2090660.8120797
5907740.8100386mitogen-activated protein kinase 13
14350030.8098225tumor suppressing subtransferable candidate 1
5027740.8091404hypothetical protein FLJ20623
4885050.808712accessory proteins BAP31/BAP29
1098630.8066906epithelial membrane protein 2
8134190.8043966“hydroxyacyl-Coenzyme A dehydrogenase, type II”
18451690.8033348“RAB35, member RAS oncogene family”
7427070.7955239“ESTs, Weakly similar to MUC2_HUMAN MUCIN 2
PRECURSOR [H. sapiens]”
5945000.7935958EST
14567010.7934389B-cell CLL/lymphoma 9
2714720.7901735C3HC4-like zinc finger protein
14739220.7892502“actin related protein 2/3 complex, subunit 3 (21 kD)”
517730.7858986hypothetical protein MGC3077
8980320.7852733KIAA0097 gene product
3405580.7791736“actin related protein 2/3 complex, subunit 5 (16 kD)”
7739220.7767604KIAA0005 gene product
7680640.7743382“cytochrome P450, subfamily I (aromatic compound-
inducible), polypeptide 1”
6259230.7725056phosphoenolpyruvate carboxykinase 2 (mitochondrial)
1500030.77183hypothetical protein FLJ13187
7419770.7715372“B-factor, properdin”
3657380.7709456ESTs
8143500.7701748insulin-degrading enzyme
21080770.7700354CGI-112 protein
2090660.7693907serine/threonine kinase 15
5644920.7672548mitochondrial carrier homolog 2
3663530.7664569DKFZP564C186 protein
8137510.7659116“sialyltransferase 4C (beta-galactosidase alpha-2,3-
sialytransferase)”
7810970.7633668reticulon 3
2333490.762952hypothetical protein FLJ10761
8253270.7622414“Homo sapiens cDNA FLJ14105 fis, clone
MAMMA1001202”
2486490.761865hypothetical protein FLJ13910
5892320.7583348hypothetical protein FLJ11506
5648470.757516ESTs
7546280.7573763ESTs
15001620.7571399ESTs
17382080.7565056“a disintegrin-like and metalloprotease (reprolysin type)
with thrombospondin type 1 motif, 4”
8429940.7563101cathepsin Z
424080.7559848hypothetical protein MGC4604
4701240.7539249RAD1 (S. pombe) homolog
8093570.7535411Bernardinelli-Seip congenital lipodystrophy 2 (seipin)
6246670.7524325CGI-92 protein
493510.75115SEX gene
462480.7510436ADP-ribosyltransferase (NAD+; poly (ADP-ribose)
polymerase)
6865520.7506994golgi phosphoprotein 1
16317350.7505843“Homo sapiens, clone IMAGE: 3604336, mRNA, partial cds”
15360060.7499887ESTs
833630.748646protein-L-isoaspartate (D-aspartate) O-methyltransferase
7137820.7484589a disintegrin and metalloproteinase domain 15 (metargidin)
7270780.7472345“Homo sapiens cDNA: FLJ23602 fis, clone LNG15735”
5038890.7454098hypothetical protein FLJ10656
2842610.7445419uncharacterized hematopoietic stem/progenitor cells
protein MDS030
14732890.7441483protective protein for beta-galactosidase (galactosialidosis)
492730.742123“solute carrier family 27 (fatty acid transporter), member 4”
7857010.7413837“RAB31, member RAS oncogene family”
415690.7412357hypothetical protein FLJ12650
8104020.7407907hypothetical protein
14563480.7397574N-acetylneuraminic acid phosphate synthase; sialic acid
synthase
2566190.7395032hydroxysteroid (17-beta) dehydrogenase 7
7435890.7372744“ESTs, Weakly similar to T2D3_HUMAN TRANSCRIPTION
INITIATION FACTOR TFIID 135 KDA SUBUNIT
[H. sapiens]”
14095090.7370811“troponin T1, skeletal, slow”
1222410.7352191“proteasome (prosome, macropain) subunit, beta type, 2”
4700990.7343404HT002 protein; hypertension-related calcium-regulated
gene
7254540.7335223CDC28 protein kinase 2
7966940.7317383baculoviral IAP repeat-containing 5 (survivin)
1867680.7298378“Homo sapiens, clone IMAGE: 3604869, mRNA”
8136290.7288266YME1 (S. cerevisiae)-like 1
8429800.7284256developmentally regulated GTP-binding protein 1
7699210.726646ubiquitin carrier protein E2-C
23223670.7249854reticulon 4
9512330.7240615“proteasome (prosome, macropain) subunit, beta type, 3”
8428250.7235007G1 to S phase transition 1
7826080.7230962
20431670.7224903BCL2-associated athanogene 3
8271710.7211896ESTs
1202710.7208543hypothetical protein MGC4692
15188900.7206844“metallothionein-like 5, testis-specific (tesmin)”
605650.720547lethal giant larvae (Drosophila) homolog 2
20508270.7156518“proteasome (prosome, macropain) 26S subunit, ATPase,
5”
16356810.7152705“NADH dehydrogenase (ubiquinone) 1 alpha subcomplex,
2 (8 kD, B8)”
7310230.7151176WD repeat domain 5
15184020.7150061KIAA1361 protein
7526310.7139862“fibroblast growth factor receptor 3 (achondroplasia,
thanatophoric dwarfism)”
7856160.7131593“signal sequence receptor, alpha (translocon-associated
protein alpha)”
6861720.7131363KIAA0008 gene product
401730.7124913KIAA0807 protein
1234410.7124698ribosomal protein L7a
1236140.7117834hypothetical protein MGC4675
8110240.7117211bone marrow stromal cell antigen 2
23020990.7117055sialidase 3 (membrane sialidase)
7564420.7115925P450 (cytochrome) oxidoreductase
8115850.7087617huntingtin (Huntington disease)
2799700.7040135adenosine A2a receptor
15171710.7036866“interleukin 2 receptor, alpha”
8383660.7026173-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
(hydroxymethylglutaricaciduria)
8099440.7021108KIAA0310 gene product
13930180.701717“general transcription factor IIIC, polypeptide 1 (alpha
subunit, 220 kD )”
7259780.7010756“ESTs, Moderately similar to ALU7_HUMAN ALU
SUBFAMILY SQ SEQUENCE CONTAMINATION
WARNING ENTRY [H. sapiens]”
3256060.7008219hypothetical protein MGC14353
7952560.6974866NPD007 protein
3653580.696963pM5 protein
677650.6967324carboxypeptidase M
3582670.6962436“EST, Moderately similar to AF119917 63 PRO2831
[H. sapiens]”
1493550.6943923translocating chain-associating membrane protein
2125420.6938079“Homo sapiens cDNA FLJ12900 fis, clone
NT2RP2004321”
795200.6919579“RAB2, member RAS oncogene family”
7391260.6881663tissue specific transplantation antigen P35B
2025140.6872212DNA (cytosine-5-)-methyltransferase 3 alpha
18844040.6864546KIAA0285 gene product
8143780.6857148“serine protease inhibitor, Kunitz type, 2”
20183370.6828744“glucosidase, beta; acid (includes glucosylceramidase)”
7017510.6824676cut (Drosophila)-like 1 (CCAAT displacement protein)
20180840.6822251Ste-20 related kinase
7810190.6819736paraoxonase 2
22441960.6807351B-cell receptor-associated protein BAP29
1244470.679866K1AA1184 protein
7890120.6794432fibulin 2
4907780.6788304low molecular mass ubiquinone-binding protein (9.5 kD)
20115150.6774452DKFZP586B0923 protein
807640.6765616hypothetical protein
8416790.6762195calcium and integrin binding protein (DNA-dependent
protein kinase interacting protein)
1084250.6761526
8784060.6750777metaxin 1
4877330.6745496
6256930.6744622hypothetical protein MGC10911
13258160.6742078polymerase (RNA) II (DNA directed) polypeptide L (7.6 kD)
2785310.6737637cytochrome c oxidase subunit VIc
7443740.6732551putative ankyrin-repeat containing protein
2030030.6724027“non-metastatic cells 4, protein expressed in”
5649810.6712134ESTs
8129940.671102“retinoid X receptor, alpha”
1725170.6701228hippocalcin-like 1
2050490.6682666protein kinase H11; small stress protein-like protein HSP22
17343090.6672454sperm associated antigen 4
16316990.6665952valosin-containing protein
20289490.6662774hypothetical protein PRO1855
5301970.6659784“ESTs, Moderately similar to ALU8_HUMAN ALU
SUBFAMILY SX SEQUENCE CONTAMINATION
WARNING ENTRY [H. sapiens]”
1996450.6654138nicastrin
7042540.6653635a disintegrin and metalloproteinase domain 8
15927150.6637095“Homer, neuronal immediate early gene, 3”
3020310.6626629Ste20-related serine/threonine kinase
2395680.661735annexin A9
7707850.6613519“1,2-alpha-mannosidase IC”
7857950.660789hypothetical protein FLJ12910
8107340.6603412“polymerase (DNA-directed), delta 4”
16967570.6591801hypothetical protein KIAA1165
8117610.6587469Nijmegen breakage syndrome 1 (nibrin)
1503140.6584621lysophospholipase I
4893510.65846hypothetical protein DKFZp566J2046
5934310.6578268“ESTs, Moderately similar to CEGT_HUMAN CERAMIDE
GLUCOSYLTRANSFERASE [H. sapiens]”
375540.6574292hypothetical protein FLJ22353
1268510.6559601hypothetical protein FLJ11160
2651030.6548137Homo sapiens mRNA; cDNA DKFZp547M123 (from clone
DKFZp547M123)
510830.6544472“catenin (cadherin-associated protein), delta 2 (neural
plakophilin-related arm-repeat protein)”
4315050.6534315“ESTs, Highly similar to A31026 probable membrane
receptor protein [H. sapiens]”
14203700.6531171biliverdin reductase B (flavin reductase (NADPH))
14760530.6522237RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
8824840.6521026“chaperonin containing TCP1, subunit 7 (eta)”
515320.6504769ADP-ribosylation factor-like 6 interacting protein
2802490.6503028Kruppel-like factor 7 (ubiquitous)
1387880.6497728prolactin receptor
14924680.647846DEME-6 protein
2212950.6477514“inhibitor of DNA binding 2, dominant negative helix-loop-
helix protein”
8978060.6470379“hypoxia-inducible factor 1, alpha subunit (basic helix-loop-
helix transcription factor)”
3589360.6441198“ESTs, Weakly similar to T2D3_HUMAN TRANSCRIPTION
INITIATION FACTOR TFIID 135 KDA SUBUNIT
[H. sapiens]”
8408650.644011“myristoylated alanine-rich protein kinase C substrate
(MARCKS, 80K-L)”
7883340.6427785mitochondrial ribosomal protein L23
3660670.6416356cerebellar degeneration-related protein (62 kD)
8255850.6413235tubulin-specific chaperone e
14601100.6397172“proteasome (prosome, macropain) subunit, beta type, 5”
3440910.6381862ESTs
1961890.6338517cytochrome b-5
22484880.6337488ems1 sequence (mammary tumor and squamous cell
carcinoma-associated (p80/85 src substrate)
5098230.6334191carcinoembryonic antigen-related cell adhesion molecule 6
(non-specific cross reacting antigen)
1448800.6332999hypothetical protein from EUROIMAGE 1759349
7555990.6330221interferon induced transmembrane protein 1 (9-27)
2072880.632639insulin induced gene 1
16395310.6315732“RAB27A, member RAS oncogene family”
1447400.630134similar to phosphatidylcholine transfer protein 2
8143060.630078tumor protein D52
3011220.6299499extracellular matrix protein 1
1242980.6294358microsomal glutathione S-transferase 3
17332620.6293971BLu protein
3768750.6286902flavin containing monooxygenase 1
7731880.6270494“nuclear receptor subfamily 1, group D, member 2”
7566660.6269882“protein phosphatase 1, catalytic subunit, alpha isoform”
8101560.6266313deoxythymidylate kinase (thymidylate kinase)
20155170.6259564hypothetical protein FLJ22237
7708450.6253839hexokinase 1
8137070.6252347regulator of G-protein signalling 16
6114430.6244565myoglobin
1243310.624455“cleavage and polyadenylation specific factor 5, 25 kD
subunit”
8558000.6235647prolyl endopeptidase
6252340.62188KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein
retention receptor 3
7533780.6218232hypothetical protein FLJ22649 similar to signal peptidase
SPC22/23
20558070.6216372protein kinase domains containing protein similar to
phosphoprotein C8FW
8408780.6216124seladin-1
17004360.6215741ESTs
2614720.6215341putative nuclear protein ORF1-FL49
1212510.6209436hypothetical protein MGC5576
1402890.6205892hypothetical protein
20188210.6205344ATPase inhibitor precursor
7707660.6193342DKFZP564C1940 protein
7677980.6192232“ATX1 (antioxidant protein 1, yeast) homolog 1”
8453630.617901“non-metastatic cells 1, protein (NM23A) expressed in”
1207490.6172281“ESTs, Moderately similar to KIAA1215 protein [H. sapiens]”
3581620.616765protein predicted by clone 23627
7813420.6159353hypothetical protein MGC11115
7677610.6142782DKFZP434B168 protein
1919040.6141265“BUB3 (budding uninhibited by benzimidazoles 3, yeast)
homolog”
7266990.6131491“ESTs, Weakly similar to AAB47496 NG5 [H. sapiens]”
23260570.61263MLN51 protein
7841400.6102245WD repeat domain 15
7703550.6099761“lanosterol synthase (2,3-oxidosqualene-lanosterol
cyclase)”
813360.6099072uteroglobin
7573280.6097389hypothetical protein FLJ22678
7736740.6095732oncogene TC21
2899780.6090234ubiquitin-like 4
665350.6088919ornithine decarboxylase antizyme 2
8263630.6087183lysophospholipase II
17585900.6074571“fatty-acid-Coenzyme A ligase, long-chain 3”
15533060.6066787“proteasome (prosome, macropain) 26S subunit, non-
ATPase, 11”
7857660.6058332hypothetical protein
7258410.6051304KIAA0662 gene product
20208980.6044163“procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3”
5043080.6041122hypothetical protein FLJ10540
5105750.6040713hypothetical protein FLJ22087
496300.6027878“calcium channel, voltage-dependent, L type, alpha 1D
subunit”
1425860.602037MCT-1 protein
7252840.6014099“phosphorylase kinase, gamma 2 (testis)”
4297990.6012713hypothetical protein FLJ21939 similar to 5-azacytidine
induced gene 2
14744240.6006702“Homo sapiens cDNA FLJ12758 fis, clone
NT2RP2001328”
7841050.6003082ESTs
20185270.599584dolichyl-phosphate mannosyltransferase polypeptide 3
8555630.5987092v-erb-b2 avian erythroblastic leukemia viral oncogene
homolog 3
8235740.5978052endosulfine alpha
3236930.5975785“adaptor-related protein complex 1, sigma 1 subunit”
8249620.595868“karyopherin alpha 2 (RAG cohort 1, importin alpha 1)”
7734260.5941568KIAA0391 gene product
7729250.5940171HSPCO34 protein
16560620.593408coagulation factor XII (Hageman factor)
8257400.5931142DKFZp434J1813 protein
3765160.5906248cell division cycle 4-like
2922130.5883092“guanine nucleotide binding protein (G protein), beta
polypeptide 2”
18986190.5880791hypothetical protein MGC15737
4149920.587733K562 cell-derived leucine-zipper-like protein 1
15739460.5875624programmed cell death 9
7391090.5874334“adaptor-related protein complex 2, sigma 1 subunit”
4302350.585372“H2B histone family, member Q”
25721700.5852444“ESTs, Weakly similar to T26581 hypothetical protein
Y32B12A.3 - Caenorhabditis elegans [C. elegans]”
487733-20.5844776“NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2
(8 kD, AGGG)”
16980360.5843382ubiquitin-conjugating enzyme E2 variant 1
1276460.5838788
3466960.5838269TEA domain family member 4
747380.5835427“Homo sapiens, clone IMAGE: 3535294, mRNA, partial cds”
1247810.5832065squalene epoxidase
18830280.5828007Homo sapiens mRNA; cDNA DKFZp434J1912 (from clone
DKFZp434J1912)
1832000.5801312fumarylacetoacetate hydrolase (fumarylacetoacetase)
517410.5795151GTP-binding protein
5907590.5791873sterol-C4-methyl oxidase-like
7553010.5790795“protein kinase C, delta”
8430540.5776102KIAA1533 protein
5952130.5775511hypothetical protein
7526430.5774681group XII secreted phospholipase A2
1418520.5762886“purinergic receptor P2Y, G-protein coupled, 2”
8136310.5754418seven transmembrane protein TM7SF3
16019470.5744444cytochrome c oxidase subunit VIIa polypeptide 2 (liver)
516570.5736969hypothetical protein ET
8142090.5736866ESTs
720500.5722845“chloride channel, nucleotide-sensitive, 1A”
16356650.5714089“Homo sapiens, RIKEN cDNA 2010100O12 gene, clone
MGC: 14813 IMAGE: 4133274, mRNA, complete cds”
664060.5706229hypothetical protein DKFZp762E1312
5303100.5686743KIAA0143 protein
413560.5683316“protein phosphatase 2, regulatory subunit B (B56), alpha
isoform”
18447650.5670398Homo sapiens mRNA; cDNA DKFZp564O1763 (from clone
DKFZp564O1763)
4898230.5666593“COX17 (yeast) homolog, cytochrome c oxidase assembly
protein”
15177490.5665848ESTs
8134100.5664086polymerase (RNA) II (DNA directed) polypeptide K (7.0 kD)
14167820.5657017“creatine kinase, brain”
14223380.5652946ribonucleotide reductase M2 polypeptide
2503130.5649247ESTs
2359860.5649127“wingless-type MMTV integration site family, member 11”
663170.5648355“H1 histone family, member 2”
2797200.5645072“ESTs, Moderately similar to A47582 B-cell growth factor
precursor [H. sapiens]”
8844980.5640535uncharacterized hypothalamus protein HT012
5038510.564048nuclear receptor co-repressor/HDAC3 complex subunit
8239300.5633774“actin related protein 2/3 complex, subunit 1A (41 kD)”
1202710.5626611hypothetical protein MGC4692
7825130.5625811“interferon, alpha-inducible protein (clone IFI-6-16)”
2468000.5625675hypothetical protein FLJ10803
23090730.5617024frizzled (Drosophila) homolog 5
7841500.5598483“RAB31, member RAS oncogene family”
1398350.5590106UDP-glucose dehydrogenase
16418940.5587185EST
7967570.5585876“adaptor-related protein complex 3, sigma 1 subunit”
8136160.5574238FK506-binding protein like
25496340.5572052activator of S phase kinase
7417690.5565863“polymerase (DNA directed), beta”
4886420.5560728“ESTs, Weakly similar to I38022 hypothetical protein
[H. sapiens]”
7954980.5557245putative transmembrane protein
14927800.5555908“Homo sapiens cDNA FLJ14459 fis, clone
HEMBB1002409”
2410430.5545302“Human clone 137308 mRNA, partial cds”
2959860.5544422emopamil-binding protein (sterol isomerase)
8396820.5542571ubiquitin-conjugating enzyme E2N (homologous to yeast
UBC13)
15819410.5537015hypothetical protein FLJ14540
8094660.5532437DNA segment on chromosome 19 (unique) 1177
expressed sequence
19473810.5524634hypothetical protein FLJ22329
8254700.5523318topoisomerase (DNA) II alpha (170 kD)
18489770.5519913glycerol kinase
7007920.5493223cyclin-dependent kinase inhibitor 3 (CDK2-associated dual
specificity phosphatase)
8253860.5487104“ATP synthase, H+ transporting, mitochondrial F1F0,
subunit d”
8261940.5486839synaptotagmin-like 2
2998150.5485843hypothetical protein DC42
17534970.5485231ovo (Drosophila) homolog-like 1
7449440.5482368myosin VI
14998280.5479012“fucosyltransferase 1 (galactoside 2-alpha-L-
fucosyltransferase, Bombay phenotype included)”
4151020.5465851cell division cycle 25C
8133870.5460577diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
7862650.5443881KIAA0750 gene product
15912640.5440944transaldolase 1
7565950.5433619“S100 calcium-binding protein A10 (annexin II ligand,
calpactin I, light polypeptide (p11))”
8557490.5432466triosephosphate isomerase 1
4700920.5429717like-glycosyltransferase
2447640.5424555B7 homolog 3
16372820.542121hexokinase 2
4178010.5414761mitochondrial ribosomal protein L27
8260770.5405591pyruvate dehydrogenase (lipoamide) beta
7574890.5386267“tubulin, alpha 2”
3276350.5368033adenylate kinase 1
3437310.5365018
7860670.5361882cell division cycle 25B
20174150.5353408centromere protein A (17 kD)
2929960.5341429“tyrosine 3-monooxygenase/tryptophan 5-monooxygenase
activation protein, eta polypeptide”
8157810.5340254heat shock 105 kD
3469420.5326646“phosphatidylinositol glycan, class Q”
8236940.5321203“Homo sapiens chromosome 19, BAC CIT-HSPC_204F22
(BC228680), complete sequence; contains bacterial
insertion element”
1393540.5318709hypothetical protein
2214990.5317861KIAA0508 protein
2637270.5313082“DNA segment, single copy probe LNS-CAI/LNS-CAII
(deleted in polyposis”
4895940.5308806hypothetical protein FLJ11565
8245240.5296138UDP-galactose transporter related
9512160.5291832“NADH dehydrogenase (ubiquinone) 1 beta subcomplex,
10 (22 kD, PDSW)”
8412600.5285587hypothetical protein
2837390.5276555“Homo sapiens cDNA FLJ14028 fis, clone
HEMBA1003838”
491170.5274035KIAA0215 gene product
17621110.5272967natriuretic peptide receptor C/guanylate cyclase C
(atrionatriuretic peptide receptor C)
3586090.5266332NADH dehydrogenase (ubiquinone) flavoprotein 3 (10 kD)
8844250.5265114“chaperonin containing TCP1, subunit 5 (epsilon)”
7954010.5264278diacylglycerol O-acyltransferase (mouse) homolog
8119070.5246416hypothetical protein FLJ22056
16317470.5245873male-enhanced antigen
3650600.5244569“RAB11A, member RAS oncogene family”
7432200.5242028hypothetical protein FLJ12517
7050640.5238148“transforming, acidic coiled-coil containing protein 3”
7684520.5237494“Homo sapiens EST from clone 491476, full insert”
7439770.5237158Homo sapiens mRNA for TL132
15688250.5205165Arg/Abl-interacting protein ArgBP2
7728980.520392ribosomal protein S15a
3668340.5191005envoplakin
16162530.5190257breast carcinoma amplified sequence 1
3226170.5188645v-ral simian leukemia viral oncogene homolog B (ras
related; GTP binding protein)
8252960.5178447low density lipoprotein receptor defect C complementing
4915240.5175752mitochondrial ribosomal protein L13
1434260.5173212“ras homolog gene family, member B”
730090.516678“ESTs, Weakly similar to A43932 mucin 2 precursor,
intestinal [H. sapiens]”
2935690.5162069chromosome 1 open reading frame 21
2967020.5162041“deiodinase, iodothyronine, type I”
7732860.5158979“solute carrier family 9 (sodium/hydrogen exchanger),
isoform 3 regulatory factor 1”
7266580.5157542“non-metastatic cells 3, protein expressed in”
7538970.5153412autocrine motility factor receptor
2937270.5153298hypothetical protein MGC861
8109470.5151346“LIS1-interacting protein NUDE1, rat homolog”
4914650.5150527hypothetical protein FLJ10035
8148990.5142481BCL2/adenovirus E1B 19 kD-interacting protein 3-like
2665000.5135558ESTs
11599630.5133656interferon regulatory factor 7
7857070.5133005protein regulator of cytokinesis 1
3461340.5132068calcium-regulated heat-stable protein (24 kD)
4861100.5127183profilin 2
20174030.5117711regulator of G-protein signalling 3
4915270.5116183“Homo sapiens, Similar to CG7083 gene product, clone
MGC: 10534 IMAGE: 3957147, mRNA, complete cds”
7696000.5106262uracil-DNA glycosylase 2
15582330.5101529ESTs
6490840.5099122carbonic anhydrase XI
8258220.5097808hypothetical protein
14614770.509331Homo sapiens mRNA; cDNA DKFZp586I0324 (from clone
DKFZp586I0324)
3275060.5092192Homo sapiens mRNA full length insert cDNA clone
EUROIMAGE 327506
7699420.509086kinesin-like 4
5052890.5080495“angiotensin II, type I receptor-associated protein”
7264390.5080059CGI-143 protein
797260.5078677“ESTs, Highly similar to T46395 hypothetical protein
DKFZp434I1120.1 [H. sapiens]”
8107620.5072899SNARE protein
7857930.5072717“capping protein (actin filament) muscle Z-line, alpha 1”
815990.5067033ubiquitin specific protease 14 (tRNA-guanine
transglycosylase)
2943970.5066683DKFZP586A0522 protein
19476470.5064941CGI-147 protein
7958050.5064099KIAA0332 protein
8109590.505619Rho GDP dissociation inhibitor (GDI) alpha
23222230.5047814small nuclear ribonucleoprotein polypeptide A
4721030.503987“soc-2 (suppressor of clear, C. elegans) homolog”
8106090.5036493hypothetical protein PP1226
8978130.5034689polyadenylate binding protein-interacting protein 1
15005420.5032679regulator of G-protein signalling 11
8397460.5031787“Homo sapiens, Similar to RIKEN cDNA 5830420C20
gene, clone IMAGE: 3633379, mRNA, partial cds”
3436070.5029133AD-015 protein
6499770.5002695Homo sapiens clone CDABP0014 mRNA sequence
8239400.4997308“transducer of ERBB2, 1”
22508390.4994848androgen receptor (dihydrotestosterone receptor; testicular
feminization; spinal and bulbar muscular atrophy; Kennedy
disease)
7310800.4979864hypothetical protein FLJ12661
7534000.496254CGI-204 protein
8693750.4958886“isocitrate dehydrogenase 2 (NADP+), mitochondrial”
1546100.4948571dynactin 4
1308350.4946783“Homo sapiens, Similar to clone FLB3816, clone
IMAGE: 3454380, mRNA”
8592280.4942316“isocitrate dehydrogenase 1 (NADP+), soluble”
416980.4938629progesterone binding protein
301700.4936252“caspase 3, apoptosis-related cysteine protease”
2662180.4934791hypothetical protein FLJ11350
5095880.49319“TATA box binding protein (TBP)-associated factor, RNA
polymerase II, J, 20 kD”
19165750.4923447BCL2-interacting killer (apoptosis-inducing)
7449940.4916322hypothetical protein FLJ12242
4874440.4887966“cyclic AMP phosphoprotein, 19 kD”
5038660.4884193sperm autoantigenic protein 17
8143530.4883684phorbol-12-myristate-13-acetate-induced protein 1
8155010.4879053hypothetical protein MGC2721
7461630.4863317“ESTs, Weakly similar to ALU1_HUMAN ALU SUBFAMILY
J SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]”
3477260.4837701homeo box D8
8977700.4836347
18545390.4824023GAP-associated tyrosine phosphoprotein p62 (Sam68)
411230.481642“Homo sapiens, Similar to RIKEN cDNA 2210021G21
gene, clone MGC: 14859 IMAGE: 3621871, mRNA,
complete cds”
8564470.4816395“interferon, gamma-inducible protein 30”
15685610.4808662BCL2-like 1
7440470.4802334polo (Drosophia)-like kinase
2901010.4800598ESTs
4552750.4788778hypothetical protein FLJ23469
20696020.4786416melanocortin 1 receptor (alpha melanocyte stimulating
hormone receptor)
5928010.4785497“serine palmitoyltransferase, long chain base subunit 2”
7418910.4775456“RAB2, member RAS oncogene family-like”
14349480.4771454HIV TAT specific factor 1
4306140.476288“2,3-bisphosphoglycerate mutase”
4548960.4760701“DnaJ (Hsp40) homolog, subfamily A, member 2”
2689460.4751692WD40 protein Ciao1
15727100.4750848hypothetical protein FLJ21213
6263180.4746692ubinuclein 1
3773840.4746085“nuclear receptor subfamily 2, group F, member 2”
16322520.474559“complement component 1, q subcomponent, alpha
polypeptide”
4894440.4741367hypothetical protein FLJ20211
3457870.4734963“highly expressed in cancer, rich in leucine heptad repeats”
7674870.4729158“ariadne(Drosophila) homolog, ubiquitin-conjugating
enzyme E2-binding protein, 1”
empty-310.4726918blank
1356400.4708685syntaxin 3A
7546530.4707927“cadherin, EGF LAG seven-pass G-type receptor 3,
flamingo (Drosophila) homolog”
15576370.4705061ESTs
7886410.4703576“adaptor-related protein complex 1, sigma 2 subunit”
8105670.4702908“Homo sapiens, clone MGC: 3182 IMAGE: 3356293, mRNA,
complete cds”
3660390.4701924KIAA0892 protein
7810470.4699376budding uninhibited by benzimidazoles 1 (yeast homolog)
7826880.4694868“dynein, axonemal, light intermediate polypeptide”
2108620.4692392“acyl-Coenzyme A oxidase 1, palmitoyl”
5107940.4691467c-myc binding protein
1214360.4691298“Homo sapiens, clone MGC: 4677 IMAGE: 3532809, mRNA,
complete cds”
3255150.4689353hypothetical protein FLJ10980
9652230.4687291“thymidine kinase 1, soluble”
14691480.467634FGFR1 oncogene partner
1495390.4671676KIAA1700
23069870.4670155secreted and transmembrane 1
1221470.4652151
20639820.4651856“potassium channel, subfamily K, member 6 (TWIK-2)”
7698900.4647115nucleoside phosphorylase
16424960.4645092hypothetical protein MGC11266
4285820.4642013hypothetical protein FLJ20296
665990.4639123N-acetyltransferase 1 (arylamine N-acetyltransferase)
15654550.4634802“nuclear receptor subfamily 2, group C, member 2”
8109390.4634658hypothetical protein FLJ22169
3597230.4630712mitochondrial elongation factor G
10272830.4628012ESTs
16368440.4627748ring finger protein 14
4691510.4622121“eukaryotic translation initiation factor 2, subunit 2 (beta,
38 kD)”
8136750.4614894“Human D9 splice variant B mRNA, complete cds”
4896570.4609514tryptophan rich basic protein
4693830.4607375chromosome 8 open reading frame 1
400420.4604322hypothetical protein FLJ10747
7266370.45959t-complex-associated-testis-expressed 1-like
8111420.4595393“phosphoinositide-3-kinase, regulatory subunit, polypeptide
2 (p85 beta)”
1792120.4594831“ESTs, Moderately similar to T12539 hypothetical protein
DKFZp434J154.1 [H. sapiens]”
2929360.4593712hypothetical protein FLJ10468
20466790.4579596ESTs
8104970.4572069“ESTs, Weakly similar to A35363 synapsin I splice form a
[H. sapiens]”
15854920.4571478ESTs
7826890.4570254“solute carrier family 6 (neurotransmitter transporter,
creatine), member 8”
7744460.4567242adrenomedullin
3661560.4563893“Homo sapiens cDNA FLJ14028 fis, clone
HEMBA1003838”
7256490.4559473“nuclear factor of activated T-cells, cytoplasmic,
calcineurin-dependent 4”
763620.4553121“spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)”
7540460.4550413DNA segment on chromosome X (unique) 9879 expressed
sequence
3455380.4550133cathepsin L
7824390.454474“ATP synthase, H+ transporting, mitochondrial F0 complex,
subunit e”
15370010.4539586ESTs
3454230.4538841DKFZP564M112 protein
17027420.453453“solute carrier family 7 (cationic amino acid transporter, y+
system), member 5”
8256590.4515758N-myc downstream regulated
16523100.4512689“Homo sapiens, clone MGC: 19613 IMAGE: 3833049,
mRNA, complete cds”
15232250.4511104oncostatin M receptor
407730.4510289“guanine nucleotide binding protein (G protein), alpha z
polypeptide”
356260.4509825“Homo sapiens cDNA FLJ14201 fis, clone
NT2RP3002955”
3582170.4507923glypican 4
2149960.45033ESTs
1778270.4500254synaptotagmin VII
10756350.4495618MLSN1- and TRP-related
8100390.4491986defender against cell death 1
7722200.4475721for protein disulfide isomerase-related
706060.4475592ESTs
16921950.4471181smg GDS-ASSOCIATED PROTEIN
8416210.4466003“ESTs, Weakly similar to JE0350 Anterior gradient-2
[H. sapiens]”
8557070.4456433ESTs
14553940.4455695cytochrome c
858040.44449hypothetical protein FLJ21918
8095120.4442565hypothetical protein FLJ10767
7534570.4435792NADH dehydrogenase (ubiquinone) Fe-S protein 1 (75 kD)
(NADH-coenzyme Q reductase)
7672890.4432685hypothetical protein FLJ10055
5652350.4428854spermine synthase
6114670.4428543NADH dehydrogenase (ubiquinone) Fe-S protein 6 (13 kD)
(NADH-coenzyme Q reductase)
8121050.4424976ALL1-fused gene from chromosome 1q
15586420.4424721hypothetical protein MGC2771
8149890.4419384“protein phosphatase 1G (formerly 2C), magnesium-
dependent, gamma isoform”
18993120.4418248ESTs
14865330.441804keratin 16 (focal non-epidermolytic palmoplantar
keratoderma)
15650790.4415966B-cell linker
7836810.4412503upstream regulatory element binding protein 1
14227230.4411931interferon-induced protein 35
7853680.4411742PDZ-binding kinase; T-cell originated protein kinase
15175950.4409591KIAA0175 gene product
7138620.4400704ubiquitin-protein isopeptide ligase (E3)
833580.4397161ESTs
812030.4391758paraoxonase 3
740700.4390933endosulfine alpha
23062210.4385881“wingless-type MMTV integration site family, member 10B”
1406350.4384907ESTs
1501180.4383146hypothetical protein DKFZp434F054
2140680.4383007GATA-binding protein 3
14349050.4382073homeo box B7
4503010.4380657mutL (E. coli) homolog 3
20279520.437854hypothetical protein FLJ20297
7453940.4374406“Homo sapiens cDNA: FLJ23249 fis, clone COL04196”
8241080.4369933SCAN domain-containing 1
7955430.4364791thioredoxin peroxidase (antioxidant enzyme)
15928370.4355927interferon induced transmembrane protein 2 (1-8D)
775330.435582“inositol polyphosphate-5-phosphatase, 40 kD”
15831980.4350835“ESTs, Weakly similar to S65824 reverse transcriptase
homolog [H. sapiens]”
842950.4349213interleukin 1 receptor antagonist
7709920.4346858
8378640.4345283progestin induced protein
19095740.4342055mitochondrial ribosomal protein S11
1286950.4340119“ESTs, Weakly similar to I38344 titin, cardiac muscle
[H. sapiens]”
7425810.433453“Homo sapiens cDNA FLJ10366 fis, clone
NT2RM2001420”
521030.432756hypothetical protein FLJ23045
7706750.4319407“Homo sapiens cDNA: FLJ21323 fis, clone COL02374”
7563730.4310628Rho guanine exchange factor (GEF) 16
439770.4309655KIAA0182 protein
23220790.4309341
254400.4304195“staufen (Drosophila, RNA-binding protein) homolog 2”
2077940.4297389“nuclear factor (erythroid-derived 2), 45 kD”
7461900.4291743hypothetical protein DKFZp761B1514
745660.4279059“exportin 1 (CRM1, yeast, homolog)”
761960.4278385hypothetical protein FLJ20062
8261730.4276784profilin 1
15878630.4270229acetyl-Coenzyme A acyltransferase 1 (peroxisomal 3-
oxoacyl-Coenzyme A thiolase)
20177210.4268089“ESTs, Weakly similar to 2109260A B cell growth factor
[H. sapiens]”
8094640.426456“fibroblast growth factor receptor 2 (bacteria-expressed
kinase, keratinocyte growth factor receptor, craniofacial
dysostosis 1, Crouzon syndrome, Pfeiffer syndrome,
Jackson-Weiss syndrome)”
418260.4264531ESTs
16879760.4257058“endoplasmic reticulum chaperone SIL1, homolog of yeast”
4164360.4252319mitochondrial ribosomal protein L24
1454910.4250102protocadherin 1 (cadherin-like 1)
8103160.4245985very long-chain acyl-CoA synthetase; lipidosin
6299440.4242364myosin VB
16373020.424164DNAJ domain-containing
7546250.4236648“ATPase, Class II, type 9A”
2984170.4235025trefoil factor 3 (intestinal)
478530.4231314“aldehyde dehydrogenase 4 family, member A1”
8097270.4224266unc-51 (C. elegans)-like kinase 1
7745020.4222849“protein tyrosine phosphatase, non-receptor type 12”
7858400.4219115“SEC24 (S. cerevisiae) related gene family, member D”
8239070.4218216hypothetical protein FLJ10511
16799420.4215618KIAA1053 protein
8239090.4215098
16002390.421459HSPC037 protein
851950.4207753“growth arrest and DNA-damage-inducible, gamma”
16402820.4205899KIAA1240 protein
7825030.4205398fatty acid desaturase 1
2663120.4201146“ATPase, Cu++ transporting, beta polypeptide (Wilson
disease)”
8845110.4199518cytochrome c oxidase subunit VIIb
20516970.4197703“inhibitor of kappa light polypeptide gene enhancer in B-
cells, kinase beta”
7248880.4196322“cytochrome P450, subfamily IVB, polypeptide 1”
8095070.4189998hypothetical protein FLJ20568
1514490.4189856“protein tyrosine phosphatase, non-receptor type 21”
7438600.4189656“hypothetical protein, clone 2746033”
3458330.4187433heterogeneous nuclear ribonucleoprotein A/B
2599500.4187278hypothetical protein FLJ14991
2837510.4179154cortistatin
8108750.4176896“solute carrier family 26, member 6”
268830.4176151“protein kinase (cAMP-dependent, catalytic) inhibitor beta”
383560.4173012“cytochrome P450, subfamily 46 (cholesterol 24-
hydroxylase)”
351910.4165847stromal cell-derived factor 2
75859−2.4150177N-myc downstream-regulated gene 2
1569187−1.9090486heparan sulfate (glucosamine) 3-O-sulfotransferase 4
345670−1.889471“ESTs, Moderately similar to I59348 CCAAT binding
transcription factor CBF subunit C - rat [R. norvegicus]”
760299−1.8654555dickkopf (Xenopus laevis) homolog 3
753071−1.8417236“Homo sapiens cDNA: FLJ22528 fis, clone HRC12825”
344720−1.7951928glycophorin C (Gerbich blood group)
810002−1.7256692“Homo sapiens, clone MGC: 19762 IMAGE: 3636045,
mRNA, complete cds”
200814−1.7140538“membrane metallo-endopeptidase (neutral endopeptidase,
enkephalinase, CALLA, CD10)”
131839−1.7087069folate receptor 1 (adult)
300632−1.6969092hypothetical protein FLJ21044 similar to Rbig1
811920−1.6924158“interleukin 11 receptor, alpha”
486683−1.6799604Homo sapiens mRNA; cDNA DKFZp564J0323 (from clone
DKFZp564J0323)
1558675−1.6577507SRY (sex determining region Y)-box 10
840266−1.6530726“Homo sapiens cDNA: FLJ22667 fis, clone HSI08385”
285377−1.6514168pellino (Drosophila) homolog 2
1469377−1.6473741lipoma HMGIC fusion partner-like 2
45099−1.6325981regucalcin (senescence marker protein-30)
1161564−1.5917197desmuslin
298122−1.5678888frizzled (Drosophila) homolog 7
796542−1.5553964ets variant gene 5 (ets-related molecule)
289760−1.5534789“ESTs, Highly similar to T00391 hypothetical protein
KIAA0612 [H. sapiens]”
22917−1.5156635Homo sapiens mRNA; cDNA DKFZp761M0111 (from clone
DKFZp761M0111)
838478−1.5059801neurocalcin delta
788234−1.4824055“inhibitor of DNA binding 4, dominant negative helix-loop-
helix protein”
377461−1.4787671“caveolin 1, caveolae protein, 22 kD”
67741−1.4786722PP2135 protein
839736−1.4745405“crystallin, alpha B”
712139−1.4736508ADP-ribosylation factor-like 7
160192−1.4695435“ESTs, Weakly similar to 2004399A chromosomal protein
[H. sapiens]”
742685−1.4440371disabled (Drosophila) homolog 2 (mitogen-responsive
phosphoprotein)
1556433−1.4191955GRO3 oncogene
1554167−1.4126653hypothetical protein FLJ14529
877621−1.3987023nGAP-like protein
1882697−1.3873266peanut (Drosophila)-like 2
72778−1.3873177“caspase 7, apoptosis-related cysteine protease”
666879−1.3624072annexin A8
69002−1.3581187PPAR (gamma) angiopoietin related protein
811848−1.3549757hypothetical protein
490023−1.3516302hypothetical protein MGC2648
781017−1.3232108early growth response 2 (Krox-20 (Drosophila) homolog)
767202−1.3217103latent transforming growth factor beta binding protein 2
291478−1.3155899runt-related transcription factor 3
757191−1.2902603ESTs
130201−1.2885369intercellular adhesion molecule 2
1635320−1.2878152“amiloride-sensitive cation channel 2, neuronal”
140574−1.2754214“small inducible cytokine subfamily D (Cys-X3-Cys),
member 1 (fractalkine, neurotactin)”
416676−1.2751042pellino (Drosophila) homolog 1
814798−1.2748689“aldehyde dehydrogenase 1 family, member A3”
611532−1.273871“troponin I, skeletal, fast”
2056139−1.2697411LIM domain protein
377275−1.2665702ataxia-telangiectasia group D-associated protein
303109−1.2557454purinergic receptor (family A group 5)
813265−1.2549431Homo sapiens mRNA; cDNA DKFZp564H1916 (from clone
DKFZp564H1916)
878836−1.2507242“secretory granule, neuroendocrine protein 1 (7B2 protein)”
1473471−1.2409979KIAA0194 protein
529843−1.2405917“ESTs, Moderately similar to JC5238 galactosylceramide-
like protein, GCP [H. sapiens]”
505864−1.2393277RalGDS-like gene
781014−1.2359239suppression of tumorigenicity 5
470393−1.2354879“matrix metalloproteinase 7 (matrilysin, uterine)”
290378−1.2346086podocalyxin-like
196435−1.2282733ESTs
71087−1.2181178“v-maf musculoaponeurotic fibrosarcoma (avian) oncogene
family, protein F”
34093−1.2156885EST
76182−1.2102812hypothetical protein DKFZp761F241
884462−1.2078038Down syndrome critical region gene 1
277571−1.2030738KIAA1706 protein
841308−1.200964“myosin, light polypeptide kinase”
162308−1.2006293ESTs
52419−1.2004086Friedreich ataxia region gene X123
488404−1.1981268Homo sapiens clone TUA8 Cri-du-chat region mRNA
712401−1.193113“phosphoinositide-3-kinase, catalytic, delta polypeptide”
289428−1.1923798“neurotrophic tyrosine kinase, receptor, type 2”
814443−1.1905978hypothetical protein MGC3232
153760−1.1793765EphB1
1917449−1.1785893“serum amyloid A4, constitutive”
1493218−1.164886hypothetical protein FLJ22297
843070−1.1647509nucleoporin 88 kD
753038−1.1629978kinesin family member C3
811088−1.1625002ephrin-B3
2106144−1.1619925regulated in glioma
796181−1.1584587growth arrest-specific 6
1257131−1.1566479ESTs
1473274−1.1557062“myosin regulatory light chain 2, smooth muscle isoform”
208718−1.1498532annexin A1
811837−1.1468554eukaryotic translation elongation factor 1 alpha 1
768571−1.1461886SRY (sex determining region Y)-box 8
1474900−1.1347123keratin 15
756708−1.1346161“potassium intermediate/small conductance calcium-
activated channel, subfamily N, member 4”
1555924−1.1342985CSR1 protein
811837−1.1269309
1878409−1.1144369catechol-O-methyltransferase
786069−1.1143906beta-site APP-cleaving enzyme
815737−1.1116223“ATP synthase, H+ transporting, mitochondrial F1 complex,
alpha subunit, isoform 1, cardiac muscle”
132857−1.0989746Homo sapiens mRNA; cDNA DKFZp586N1323 (from clone
DKFZp586N1323)
1871116−1.0975491Homo sapiens mRNA; cDNA DKFZp434C1714 (from clone
DKFZp434C1714); partial cds
133236−1.0945253“RNA binding motif protein, X chromosome”
283023−1.0825166chemokine (C—X3-C) receptor 1
2016775−1.0761731“G protein-coupled receptor, family C, group 5, member B”
897963−1.0751021phosphatidic acid phosphatase type 2A
814316−1.0654718ribosomal protein L13
47043−1.0559861tensin
625399−1.0523138hypothetical protein similar to beta-transducin family
416959−1.051868nuclear factor I/B
366100−1.0467604matrilin 2
753162−1.0416438KIAA0603 gene product
491403−1.0411657“tumor necrosis factor receptor superfamily, member 1B”
813256−1.0407652“ATP-binding cassette, sub-family B (MDR/TAP), member
1”
740620−1.0395569tropomyosin 2 (beta)
1577736−1.035115epidermal growth factor (beta-urogastrone)
70245−1.0348655Homo sapiens mRNA full length insert cDNA clone
EUROIMAGE 50374
1626996−1.0328113c-fos induced growth factor (vascular endothelial growth
factor D)
360254−1.0325653“cysteine-rich, angiogenic inducer, 61”
80344−1.0323882interleukin 7 receptor
530958−1.0316423smoothened (Drosophila) homolog
34150−1.0302576ESTs
809784−1.030253“kallikrein 6 (neurosin, zyme)”
990881−1.0289558
416434−1.0284229SCN Circadian Oscillatory Protein (SCOP)
162308−1.023071
796475−1.0217348four and a half LIM domains 3
32489−1.0196444hypothetical protein DKFZp566A1524
2504881−1.0194516signal transducer and activator of transcription 5A
188335−1.0161259“egf-like module containing, mucin-like, hormone receptor-
like sequence 2”
593023−1.0135883“dystrobrevin, beta”
139660−1.0134677ESTs
772913−1.0122192calreticulin
270826−1.0112851“Homo sapiens cDNA FLJ13329 fis, clone
OVARC1001795”
1864302−1.0099902E74-like factor 5 (ets domain transcription factor)
1587710−1.0092611period (Drosophila) homolog 1
1584540−1.0077508Homo sapiens mRNA; cDNA DKFZp586M2022 (from clone
DKFZp586M2022)
788136−1.0073983“phosphodiesterase 4B, cAMP-specific (dunce
(Drosophila)-homolog phosphodiesterase E4)”
40027−1.0057005ESTs
1856063−1.0036618tweety (Drosophila) homolog 1
209537−0.9988461zinc finger protein 221
628955−0.997687forkhead box O1A (rhabdomyosarcoma)
2095066−0.9972552“alcohol dehydrogenase 1C (class I), gamma polypeptide”
1636166−0.9969091KIAA0668 protein
882248−0.9965858transgelin
415816−0.9954308ESTs
897731−0.9927981latrophilin
868396−0.9916319“tyrosine 3-monooxygenase/tryptophan 5-monooxygenase
activation protein, eta polypeptide”
343760−0.9907562SH3 domain binding glutamic acid-rich protein like 2
1572298−0.9906095“CD3Z antigen, zeta polypeptide (TiT3 complex)”
1474337−0.986117“phosphorylase, glycogen; brain”
1609665−0.9851713BarH-like homeobox 2
2243051−0.9835274kallikrein 8 (neuropsin/ovasin)
27544−0.9834143prominin (mouse)-like 1
281190−0.9830533ESTs
161456−0.9820939serum amyloid A1
505243−0.980381“inositol 1,4,5-triphosphate receptor, type 2”
|
Example IV
[0104] Genes for Discriminating Between Normal and ADH
[0105] As shown in Table 3 below, 600 genes were identified as being able to discriminate between normal and ADH.
3TABLE 3
|
|
CloneIDWeightDescription
|
|
13234481.3237504cysteine-rich protein 1 (intestinal)
5945001.158305EST
8240521.034408chromosome 6 open reading frame 1
1495390.9878366KIAA1700
7886540.9585887growth factor receptor-bound protein 2
15177490.947675ESTs
14922380.9155471HSPC003 protein
3663530.9154768DKFZP564C186 protein
16098360.898716glutamate-ammonia ligase (glutamine synthase)
1381890.8950816Wolfram syndrome 1 (wolframin)
3449590.8945717gene for serine/threonine protein kinase
14358620.8934643antigen identified by monoclonal antibodies 12E7, F21 and O13
20143730.8820247HNK-1 sulfotransferase
237760.879215quinoid dihydropteridine reductase
2935690.8708985chromosome 1 open reading frame 21
1308350.8659688Homo sapiens, Similar to clone FLB3816, clone
IMAGE: 3454380, mRNA
2360340.8541082uncoupling protein 2 (mitochondrial, proton carrier)
4880250.8525751Kruppel-associated box protein
1788050.8524069Human DNA sequence from clone RP5-850E9 on chromosome
20. Contains part of the gene for a novel C2H2 type zinc finger
protein similar to Drosophila Scratch (Scrt), Slug and Xenopus
Snail, a novel gene similar to Drosophila CG6762, STSs, GSSs
and five CpG
7456060.8514931hypothetical protein PP591
14924630.8378401selenoprotein X, 1
5301970.8206765ESTs, Moderately similar to ALU8_HUMAN ALU SUBFAMILY
SX SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
8982220.8092364Homo sapiens clone 24418 mRNA sequence
7826080.8041049mitochondrial ribosomal protein L9
4503010.8030667mutL (E. coli) homolog 3
1961890.7992668cytochrome b-5
3631440.7924672transcription factor AP-2 beta (activating enhancer-binding
protein 2 beta)
686360.7904354hypothetical protein MGC2477
7951850.7765346xenotropic and polytropic retrovirus receptor
2557540.7720883LUNX protein; PLUNC (palate lung and nasal epithelium clone);
tracheal epithelium enriched protein
7677980.7682106ATX1 (antioxidant protein 1, yeast) homolog 1
15582330.7654043ESTs
7696000.763743uracil-DNA glycosylase 2
11565380.7512287potassium inwardly-rectifying channel, subfamily J, member 11
15878630.7507482acetyl-Coenzyme A acyltransferase 1 (peroxisomal 3-oxoacyl-
Coenzyme A thiolase)
17097910.740832BAI1-associated protein 1
7418910.7360507RAB2, member RAS oncogene family-like
7886540.7336359
8117740.7223819CGI-49 protein
17382080.7166687a disintegrin-like and metalloprotease (reprolysin type) with
thrombospondin type 1 motif, 4
1201380.7096046J domain containing protein 1
22484880.7033736ems1 sequence (mammary tumor and squamous cell
carcinoma-associated (p80/85 src substrate)
2651030.702883Homo sapiens mRNA; cDNA DKFZp547M123 (from clone
DKFZp547M123)
20541220.698129solute carrier family 11 (proton-coupled divalent metal ion
transporters), member 3
7017510.6961443cut (Drosophila)-like 1 (CCAAT displacement protein)
8115820.690137golgi phosphoprotein 2
2663120.6856592ATPase, Cu++ transporting, beta polypeptide (Wilson disease)
351470.6842172ESTs, Weakly similar to unnamed protein product [H. sapiens]
13258160.6829962polymerase (RNA) II (DNA directed) polypeptide L (7.6 kD)
3582170.6801945glypican 4
2333490.6764709hypothetical protein FLJ10761
7419770.6758385B-factor, properdin
1451320.6725186mannose-P-dolichol utilization defect 1
332670.6679827
2797200.6607182ESTs, Moderately similar to A47582 B-cell growth factor
precursor [H. sapiens]
5653190.6603668Homo sapiens mRNA; cDNA DKFZp564B1264 (from clone
DKFZp564B1264)
7711730.6594128mitochondrial ribosomal protein S21
2714720.6582514C3HC4-like zinc finger protein
7685700.6581316hypothetical protein FLJ11280
7444170.6563238carnitine acetyltransferase
8262560.6554059transmembrane 7 superfamily member 1 (upregulated in kidney)
22508390.654954androgen receptor (dihydrotestosterone receptor; testicular
feminization; spinal and bulbar muscular atrophy; Kennedy
disease)
1792120.6499845ESTs, Moderately similar to T12539 hypothetical protein
DKFZp434J154.1 [H. sapiens]
7857010.6496359RAB31, member RAS oncogene family
1867680.6495706Homo sapiens, clone IMAGE: 3604869, mRNA
5032150.6457259pilin-like transcription factor
375540.6440792hypothetical protein FLJ22353
27105240.6399293nuclear receptor coactivator 3
2842610.6398576uncharacterized hematopoietic stem/progenitor cells protein
MDS030
7417690.6390692polymerase (DNA directed), beta
7431460.6388187hypothetical protein FLJ23403
8145280.6329458Homo sapiens cDNA: FLJ22139 fis, clone HEP20959
7425950.6324947cyclin-dependent kinase 5
8100630.630827growth factor, erv1 (S. cerevisiae)-like (augmenter of liver
regeneration)
5903100.6299822Homo sapiens mRNA; cDNA DKFZp434E2321 (from clone
DKFZp434E2321); partial cds
8416790.6283163calcium and integrin binding protein (DNA-dependent protein
kinase interacting protein)
1310940.6268883Homo sapiens cDNA: FLJ21587 fis, clone COL06946
7883340.6264069mitochondrial ribosomal protein L23
15740580.62619471-acylglycerol-3-phosphate O-acyltransferase 2
(lysophosphatidic acid acyltransferase, beta)
7313080.6259858citrate synthase
8261940.6205287synaptotagmin-like 2
21105110.6203153artemin
8119070.6180993hypothetical protein FLJ22056
8143500.6173648insulin-degrading enzyme
6114430.6112713myoglobin
7826080.6105794
227780.6100322sulfortranferase family 4A, member 1
1286950.608873ESTs, Weakly similar to I38344 titin, cardiac muscle [H. sapiens]
478530.6077928aldehyde dehydrogenase 4 family, member A1
8142090.6076904ESTs
5907740.6071935mitogen-activated protein kinase 13
9698770.6064355synaptosomal-associated protein, 25 kD
14694250.6055267SRY (sex determining region Y)-box 22
3011220.6041028extracellular matrix protein 1
1994030.6027193lectin, galactoside-binding, soluble, 8 (galectin 8)
8138450.6018909RNA, U transporter 1
8103310.6014164quiescin Q6
7707660.600234DKFZP564C1940 protein
2799700.5992634adenosine A2a receptor
4907780.5957445low molecular mass ubiquinone-binding protein (9.5 kD)
2889990.5955672small protein effector 1 of Cdc42
5049590.5953946Homo sapiens mRNA; cDNA DKFZp586G0321 (from clone
DKFZp586G0321)
424080.5930231hypothetical protein MGC4604
2637270.5926363DNA segment, single copy probe LNS-CAI/LNS-CAII (deleted in
polyposis
8547630.5918867Homo sapiens cDNA FLJ11341 fis, clone PLACE1010786
7299750.5908615meningioma expressed antigen 5 (hyaluronidase)
17332620.5891897BLu protein
7303130.5887579ESTs, Weakly similar to A43932 mucin 2 precursor, intestinal
[H. sapiens]
15000000.5871787H2B histone family, member B
5950370.5870709retinoic acid induced 3
16356810.5859113NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2
(8 kD, B8)
23258040.584065695 kDa retinoblastoma protein binding protein
20148560.5837779major histocompatibility complex, class I-like sequence
401730.5771304KIAA0807 protein
7967230.576028Homo sapiens clone CDABP0014 mRNA sequence
2395680.5750382annexin A9
20185270.5732092dolichyl-phosphate mannosyltransferase polypeptide 3
16493740.5731982homogentisate 1,2-dioxygenase (homogentisate oxidase)
8093570.571929Bernardinelli-Seip congenital lipodystrophy 2 (seipin)
3661320.5711651succinate dehydrogenase complex, subunit C, integral
membrane protein, 15 kD
833580.5709886ESTs
17621110.5703701natriuretic peptide receptor C/guanylate cyclase C
(atrionatriuretic peptide receptor C)
2448010.5693836Rho guanine exchange factor (GEF) 11
1276460.5663395
398840.5659552IMP (inosine monophosphate) dehydrogenase 1
2025770.5629317histamine N-methyltransferase
5882620.5614929Homo sapiens, Similar to RIKEN cDNA 2600001A11 gene,
clone MGC: 9907 IMAGE: 3870073, mRNA, complete cds
8134190.5602324hydroxyacyl-Coenzyme A dehydrogenase, type II
19113430.559091RAB26, member RAS oncogene family
8597610.5582503poliovirus receptor-related 2 (herpesvirus entry mediator B)
3421810.557997B-cell CLL/lymphoma 2
15581080.5577768ATP-binding cassette, sub-family C (CFTR/MRP), member 8
20174030.5568495regulator of G-protein signalling 3
1223940.5554851guanine nucleotide binding protein (G protein), alpha 15 (Gq
class)
8248790.5548666hypothetical protein MGC11275
17080550.554593glioblastoma overexpressed
14567010.5545861B-cell CLL/lymphoma 9
1863010.5537115Homo sapiens cDNA FLJ12924 fis, clone NT2RP2004709
1268510.5535041hypothetical protein FLJ11160
8266220.5532463KIAA0430 gene product
1514490.5503739protein tyrosine phosphatase, non-receptor type 21
8266680.550158KIAA0274 gene product
766050.5482232nesca protein
7860720.5450231myosin IC
22441960.5432772B-cell receptor-associated protein BAP29
7044140.5431136small nuclear ribonucleoprotein polypeptides B and B1
7395780.5402953GPI-anchored metastasis-associated protein homolog
14047740.5399447parathyroid hormone-like hormone
665990.5399379N-acetyltransferase 1 (arylamine N-acetyltransferase)
3592500.5382989carbonic anhydrase IV
4882020.5378254ESTs, Weakly similar to YZ28_HUMAN HYPOTHETICAL
PROTEIN ZAP128 [H. sapiens]
2359860.5371486wingless-type MMTV integration site family, member 11
2140680.5357344GATA-binding protein 3
15005420.5341753regulator of G-protein signalling 11
4700920.5319336like-glycosyltransferase
5891150.531314matrix metalloproteinase 1 (interstitial collagenase)
8107340.5308572polymerase (DNA-directed), delta 4
6855160.5304998putative G protein-coupled receptor
1719120.5297738Homo sapiens cDNA FLJ10960 fis, clone PLACE1000564
18588920.5295858hypothetical protein MGC4825
4149920.5293379K562 cell-derived leucine-zipper-like protein 1
377080.5283276hypothetical protein MGC3101
7137820.5273402a disintegrin and metalloproteinase domain 15 (metargidin)
4715680.5255851hematological and neurological expressed 1
8122380.5237287hypothetical protein MGC4692
16317350.5228778Homo sapiens, clone IMAGE: 3604336, mRNA, partial cds
740700.5225238endosulfine alpha
7824970.5218113Homo sapiens, clone IMAGE: 3010666, mRNA, partial cds
5027740.5204735hypothetical protein FLJ20623
15171710.5204242interleukin 2 receptor, alpha
505620.5195335chromosome 8 open reading frame 4
605650.5192032lethal giant larvae (Drosophila) homolog 2
16418940.5187462EST
510830.5170561catenin (cadherin-associated protein), delta 2 (neural
plakophilin-related arm-repeat protein)
2967020.5164562deiodinase, iodothyronine, type I
20183370.5163293glucosidase, beta; acid (includes glucosylceramidase)
16054260.5150013hypothetical protein FLJ13352
14741490.5138729poliovirus receptor-related 1 (herpesvirus entry mediator C;
nectin)
262940.5137265RNB6
8100170.5135433plasminogen activator, urokinase receptor
8258220.5134094hypothetical protein
8107250.51329ATPase, H+ transporting, lysosomal (vacuolar proton pump)
21 kD
7810190.5132896paraoxonase 2
7266580.5132765non-metastatic cells 3, protein expressed in
19165750.5127478BCL2-interacting killer (apoptosis-inducing)
14691480.5125351FGFR1 oncogene partner
7267030.5118991Homo sapiens clone 23736 mRNA sequence
7259780.5108817ESTs, Moderately similar to ALU7_HUMAN ALU SUBFAMILY
SQ SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
15739460.509585programmed cell death 9
517410.5094912GTP-binding protein
1563630.5086284hypothetical protein FLJ12934
1778270.5085582synaptotagmin VII
2943970.505999DKFZP586A0522 protein
8260770.503983pyruvate dehydrogenase (lipoamide) beta
9499380.5011384cystatin C (amyloid angiopathy and cerebral hemorrhage)
19571360.5006732phenylethanolamine N-methyltransferase
20180840.4999242Ste-20 related kinase
7841400.4984039WD repeat domain 15
8109810.498146hypothetical protein FLJ20699
6272480.4972176SBBI31 protein
23020990.4967769sialidase 3 (membrane sialidase)
3020310.49677Ste20-related serine/threonine kinase
1834400.4967506arylsulfatase A
16097480.4955206hypothetical protein MGC10882
1832000.4955056fumarylacetoacetate hydrolase (fumarylacetoacetase)
23066970.4953741neuromedin B
2927700.4944242Homo sapiens, clone IMAGE: 3627860, mRNA, partial cds
8564470.494183interferon, gamma-inducible protein 30
8555630.4941473v-erb-b2 avian erythroblastic leukemia viral oncogene homolog
3
8390810.4939384homolog of yeast long chain polyunsaturated fatty acid
elongation enzyme 2
492730.4937997solute carrier family 27 (fatty acid transporter), member 4
2895050.4933344ESTs, Moderately similar to S65657 alpha-1C-adrenergic
receptor splice form 2 [H. sapiens]
7826890.4930071solute carrier family 6 (neurotransmitter transporter, creatine),
member 8
7674950.4922026GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly
syndrome)
7825370.490998Homo sapiens cDNA: FLJ22562 fis, clone HSI01814
8550290.4904632Ac-like transposable element
7052740.4904221diacylglycerol kinase, delta (130 kD)
2025140.4902228DNA (cytosine-5-)-methyltransferase 3 alpha
7256490.4878638nuclear factor of activated T-cells, cytoplasmic, calcineurin-
dependent 4
18844040.4878341KIAA0285 gene product
8252960.4858551low density lipoprotein receptor defect C complementing
8395800.4826315ESTs
2898570.4823282phenylethanolamine N-methyltransferase
4914650.4821169hypothetical protein FLJ10035
3607780.4820033
2614720.4814013putative nuclear protein ORF1-FL49
8407680.4812559ATPase, H+ transporting, lysosomal (vacuolar proton pump)
16 kD
1236140.4800042hypothetical protein MGC4675
16035830.4785029SH3 domain binding glutamic acid-rich protein like
7826880.4780253dynein, axonemal, light intermediate polypeptide
7841500.4763599RAB31, member RAS oncogene family
7708690.4762758hypothetical protein MGC2592
4552690.4761389
3653580.4756564pM5 protein
7952560.4753832NPD007 protein
7566660.474441protein phosphatase 1, catalytic subunit, alpha isoform
7677060.4731085ESTs
4885050.4727394accessory proteins BAP31/BAP29
3440730.4726399ESTs, Weakly similar to K1CI_HUMAN KERATIN, TYPE I
CYTOSKELETAL 9 [H. sapiens]
15581510.4723511basic leucine zipper transcription factor, ATF-like
8236150.471739Homo sapiens cDNA: FLJ21245 fis, clone COL01184
493510.4697521SEX gene
15417110.4694413Homo sapiens cDNA: FLJ21513 fis, clone COL05778
8686520.4682053complement component 4B
4303180.4681908parralbumin
1434260.4679928ras homolog gene family, member B
9511250.4675003peroxisomal D3,D2-enoyl-CoA isomerase
665350.466705ornithine decarboxylase antizyme 2
8132810.4659334WW domain-containing protein 1
7266990.4658249ESTs, Weakly similar to AAB47496 NG5 [H. sapiens]
14924680.4643107DEME-6 protein
14924260.4635224chromosome 19 open reading frame 3
4891060.4628623hypothetical protein FLJ11210
4884220.4626549high-mobility group 20B
2784300.4612914kinesin family member 5C
15188900.4610867metallothionein-like 5, testis-specific (tesmin)
20177560.4608709homolog of yeast MOG1
8136310.4607567seven transmembrane protein TM7SF3
15576370.4588694ESTs
2984170.4587501trefoil factor 3 (intestinal)
8104020.4583168hypothetical protein
7677610.4565666DKFZP434B168 protein
14732890.4540707protective protein for beta-galactosidase (galactosialidosis)
7731420.4530754hypothetical protein MGC2827
16523100.4525789Homo sapiens, clone MGC: 19613 IMAGE: 3833049, mRNA,
complete cds
8121430.4519186fibronectin leucine rich transmembrane protein 3
23260570.4506179MLN51 protein
10756350.4502629MLSN1- and TRP-related
8253270.4497029Homo sapiens cDNA FLJ14105 fis, clone MAMMA1001202
8098380.4466933
7865450.4453714protein phosphatase 1, regulatory (inhibitor) subunit 3B
1835560.4452493gap junction protein, alpha 4, 37 kD (connexin 37)
5038890.4433034hypothetical protein FLJ10656
7253210.44288estrogen receptor 1
7314590.4427978Homo sapiens cDNA: FLJ22296 fis, clone HRC04468
7526310.4426961fibroblast growth factor receptor 3 (achondroplasia,
thanatophoric dwarfism)
15586420.4426424hypothetical protein MGC2771
1202710.4425879hypothetical protein MGC4692
4879320.4419815synaptotagmin-like 2
15927150.4417898Homer, neuronal immediate early gene, 3
3084660.4417728GTP-binding protein Sara
8408820.441569nucleotide binding protein
8237270.4406546Homo sapiens, clone IMAGE: 2905978, mRNA, partial cds
16360920.4404649hypothetical protein FLJ20657
15050380.43988hypothetical protein FLJ20171
4909650.4389746ESTs
3466960.437234TEA domain family member 4
3450320.4360992ESTs
4877330.4356439
8106120.4352602S100 calcium-binding protein A11 (calgizzarin)
1220770.434495putative membrane protein
4178010.4325892mitochondrial ribosomal protein L27
8784060.4311455metaxin 1
19473810.4311314hypothetical protein FLJ22329
2327890.4292025polymerase (RNA) II (DNA directed) polypeptide J (13.3 kD)
8099440.4291762KIAA0310 gene product
20191010.4289318angiotensinogen (serine (or cysteine) proteinase inhibitor, clade
A (alpha-1 antiproteinase, antitrypsin), member 8)
16560620.4288838coagulation factor XII (Hageman factor)
13905840.4284863mitochondrial intermediate peptidase
16933570.4274493endothelin 2
75859−1.6337267N-myc downstream-regulated gene 2
1558675−1.4515145SRY (sex determining region Y)-box 10
753071−1.4473398Homo sapiens cDNA: FLJ22528 fis, clone HRC12825
810002−1.2720931Homo sapiens, clone MGC: 19762 IMAGE: 3636045, mRNA,
complete cds
712139−1.2371074ADP-ribosylation factor-like 7
815737−1.1857965ATP synthase, H+ transporting, mitochondrial F1 complex, alpha
subunit, isoform 1, cardiac muscle
503671−1.1528584Homo sapiens cDNA FLJ14368 fis, clone HEMBA1001122
345670−1.135654ESTs, Moderately similar to I59348 CCAAT binding transcription
factor CBF subunit C - rat [R. norvegicus]
753038−1.1168529kinesin family member C3
741139−1.1025542eyes absent (Drosophila) homolog 2
839736−1.1019142crystallin, alpha B
2013178−1.0884449death associated transcription factor 1
1577736−1.076549epidermal growth factor (beta-urogastrone)
788234−1.0610946inhibitor of DNA binding 4, dominant negative helix-loop-helix
protein
150897−1.0221539UDP-GIcNAc: betaGal beta-1,3-N-acetylglucosaminyltransferase
3
757873−1.0188169cyclin-dependent kinase 5, regulatory subunit 1 (p35)
291478−1.0093382runt-related transcription factor 3
486683−1.0075654Homo sapiens mRNA; cDNA DKFZp564J0323 (from clone
DKFZp564J0323)
290378−1.0043647podocalyxin-like
772913−0.9823487calreticulin
544639−0.9767278ESTs
814798−0.9611553aldehyde dehydrogenase 1 family, member A3
131839−0.959265folate receptor 1 (adult)
840266−0.9425178Homo sapiens cDNA: FLJ22667 fis, clone HSI08385
1555924−0.9421074CSR1 protein
756708−0.9396531potassium intermediate/small conductance calcium-activated
channel, subfamily N, member 4
838478−0.934433neurocalcin delta
742562−0.9269494chromosome 16 open reading frame 5
1469377−0.9086679lipoma HMGIC fusion partner-like 2
884462−0.9065675Down syndrome critical region gene 1
796542−0.9052403ets variant gene 5 (ets-related molecule)
32493−0.9043423integrin, alpha 6
284592−0.9019931PR01659 protein
788136−0.8998648phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-
homolog phosphodiesterase E4)
1161564−0.8865787desmuslin
1635320−0.8845852amiloride-sensitive cation channel 2, neuronal
344720−0.8822302glycophorin C (Gerbich blood group)
1534700−0.8804685KIAA0830 protein
193913−0.8724313v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
71087−0.870641v-maf musculoaponeurotic fibrosarcoma (avian) oncogene
family, protein F
300632−0.8586688hypothetical protein FLJ21044 similar to Rbig1
416676−0.8568853pellino (Drosophila) homolog 1
69002−0.8528453PPAR(gamma) angiopoietin related protein
742685−0.852598disabled (Drosophila) homolog 2 (mitogen-responsive
phosphoprotein)
140574−0.8498606small inducible cytokine subfamily D (Cys-X3-Cys), member 1
(fractalkine, neurotactin)
23831−0.8493217aldolase C, fructose-bisphosphate
470393−0.848482matrix metalloproteinase 7 (matrilysin, uterine)
1474337−0.844539phosphorylase, glycogen; brain
753301−0.8393651carcinoembryonic antigen-related cell adhesion molecule 1
(biliary glycoprotein)
51078−0.8348835ribosomal protein L44
3172883−0.8293392ESTs, Weakly similar to 1709359A dopamine D4 receptor
[H. sapiens]
877621−0.8289253nGAP-like protein
811920−0.8286379interleukin 11 receptor, alpha
156211−0.8269263ATPase, H+ transporting, lysosomal (vacuolar proton pump),
beta polypeptide, 56/58 kD, isoform 1 (Renal tubular acidosis
with deafness)
760299−0.8251086dickkopf (Xenopus laevis) homolog 3
285377−0.8214706pellino (Drosophila) homolog 2
345034−0.8161772small inducible cytokine subfamily B (Cys-X-Cys), member 14
(BRAK)
298122−0.8158372frizzled (Drosophila) homolog 7
842896−0.8123068hypothetical protein DKFZp762L0311
843070−0.8071984nucleoporin 88 kD
1257131−0.8069059ESTs
796539−0.803124KRAB-associated protein 1
139660−0.79833ESTs
666879−0.7950485annexin A8
200814−0.7920988membrane metallo-endopeptidase (neutral endopeptidase,
enkephalinase, CALLA, CD10)
2016775−0.7914834G protein-coupled receptor, family C, group 5, member B
1946448−0.790849caveolin 2
1473471−0.7906678KIAA0194 protein
1556433−0.7877792GRO3 oncogene
1636156−0.7827089hypothetical protein FLJ21709
40299−0.78007growth differentiation factor 10
1636166−0.7788972KIAA0668 protein
1609665−0.7765472BarH-like homeobox 2
250797−0.7739669hypothetical protein FLJ20038
220395−0.7687195hypothetical protein FLJ23293 similar to ARL-6 interacting
protein-2
208718−0.7660278annexin A1
41869−0.7646494hypothetical protein FLJ11017
490023−0.7621736hypothetical protein MGC2648
1607229−0.7562938tumor protein D52-like 1
133236−0.7561129RNA binding motif protein, X chromosome
785733−0.7554682hypothetical protein FLJ12892
32489−0.7521767hypothetical protein DKFZp566A1524
70201−0.7513314mitochondrial solute carrier
611481−0.7507264HMG-box transcription factor TCF-3
1554167−0.7503289hypothetical protein FLJ14529
768571−0.7476813SRY (sex determining region Y)-box 8
783698−0.7411614lipin 1
341759−0.7393558lung type-I cell membrane-associated glycoprotein
665384−0.7388404KIAA1609 protein
142259−0.7380151tumor necrosis factor alpha-inducible cellular protein containing
leucine zipper domains; Huntingtin interacting protein L;
transcrption factor IIIA-interacting protein
810057−0.7363941vasoactive intestinal peptide receptor 1
27544−0.7342174prominin (mouse)-like 1
739193−0.7338774cellular retinoic acid-binding protein 1
813256−0.7334866ATP-binding cassette, sub-family B (MDR/TAP), member 1
293964−0.7275302butyrophilin, subfamily 3, member A1
272706−0.7274769alpha2,3-sialyltransferase
209537−0.7245778zinc finger protein 221
67741−0.7229251PP2135 protein
823714−0.7215045nuclear receptor co-repressor/HDAC3 complex subunit
471196−0.7192752integral membrane protein 3
813265−0.7192289Homo sapiens mRNA; cDNA DKFZp564H1916 (from clone
DKFZp564H1916)
42681−0.718704NY-REN-25 antigen
1601845−0.7178673Ca2+-promoted Ras inactivator
725152−0.7173869hypothetical protein DKFZp762A227
22917−0.7173328Homo sapiens mRNA; cDNA DKFZp761M0111 (from clone
DKFZp761M0111)
703541−0.7118124KIAA1858 protein
529843−0.7117107ESTs, Moderately similar to JC5238 galactosylceramide-like
protein, GCP [H. sapiens]
377461−0.7093974caveolin 1, caveolae protein, 22 kD
1569187−0.7091259heparan sulfate (glucosamine) 3-O-sulfotransferase 4
1456118−0.7074166proteasome (prosome, macropain) subunit, beta type, 9 (large
multifunctional protease 2)
289760−0.7058375ESTs, Highly similar to T00391 hypothetical protein KIAA0612
[H. sapiens]
341774−0.7044768major histocompatibility complex, class I, A
75078−0.7028415ESTs
505864−0.7020337RalGDS-like gene
415191−0.700303KIAA0161 gene product
188335−0.7002579egf-like module containing, mucin-like, hormone receptor-like
sequence 2
132711−0.6985643Kruppel-like factor 5 (intestinal)
1864302−0.6943806E74-like factor 5 (ets domain transcription factor)
344854−0.692296ankyrin repeat domain 3
454970−0.6853932DKFZP434G032 protein
79629−0.6849525
767068−0.6819032DKFZP586G1517 protein
378813−0.679848secretory leukocyte protease inhibitor (antileukoproteinase)
725076−0.67980055′-nucleotidase (purine), cytosolic type B
491403−0.6760814tumor necrosis factor receptor superfamily, member 1B
43764−0.6743049hypothetical protein FLJ14033 similar to hypoxia inducible factor
3, alpha subunit
725622−0.6731681ESTs
431231−0.6692022EGF-containing fibulin-like extracellular matrix protein 2
488956−0.6682156CUG triplet repeat, RNA-binding protein 2
72778−0.6614808caspase 7, apoptosis-related cysteine protease
360254−0.6612871cysteine-rich, angiogenic inducer, 61
76182−0.6603322hypothetical protein DKFZp761F241
773487−0.6598647hypothetical protein MGC3032
162308−0.6580605ESTs
283023−0.6577611chemokine (C—X3-C) receptor 1
196992−0.6573113aldo-keto reductase family 1, member C1 (dihydrodiol
dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid
dehydrogenase)
303109−0.6571713purinergic receptor (family A group 5)
897731−0.6548064latrophilin
289428−0.6542789neurotrophic tyrosine kinase, receptor, type 2
196435−0.653913ESTs
504791−0.6537859glutathione S-transferase A4
812975−0.6527728KIAA0172 protein
488404−0.6521658Homo sapiens clone TUA8 Cri-du-chat region mRNA
345056−0.6490368KIAA1404 protein
786069−0.6484842beta-site APP-cleaving enzyme
48518−0.6455041ATP-binding cassette, sub-family A (ABC1), member 5
812048−0.6438062prion protein (p27-30) (Creutzfeld-Jakob disease, Gerstmann-
Strausler-Scheinker syndrome, fatal familial insomnia)
868396−0.6426678tyrosine 3-monooxygenase/tryptophan 5-monooxygenase
activation protein, eta polypeptide
70245−0.6422928Homo sapiens mRNA full length insert cDNA clone
EUROIMAGE 50374
40027−0.6418674ESTs
840944−0.6399619early growth response 1
809784−0.6348991kallikrein 6 (neurosin, zyme)
1881774−0.6332545KIAA1678
52419−0.6326767Friedreich ataxia region gene X123
120106−0.631375caspase 1, apoptosis-related cysteine protease (interleukin 1,
beta, convertase)
725680−0.6295778transcription factor AP-2 gamma (activating enhancer-binding
protein 2 gamma)
1493218−0.6292715hypothetical protein FLJ22297
2018423−0.6275903death-associated protein kinase 2
1486082−0.6241275heparin-binding growth factor binding protein
868169−0.6224058lipoprotein lipase
209137−0.6161588gamma-aminobutyric acid (GABA) A receptor, epsilon
248886−0.6161134rab3 GTPase-activating protein, non-catalytic subunit (150 kD)
73252−0.61156023-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
612274−0.6108452tubulin, alpha 1 (testis specific)
346545−0.6098367laminin, beta 1
84464−0.6091345hypothetical protein FLJ12806
628955−0.6088522forkhead box O1A (rhabdomyosarcoma)
490414−0.6078858chromosome 2 open reading frame 2
752837−0.6056177Homo sapiens mRNA for FLJ00074 protein, partial cds
796904−0.604755pleiomorphic adenoma gene-like 1
418159−0.6038708synaptogyrin 1
323238−0.6032171GRO1 oncogene (melanoma growth stimulating activity, alpha)
1570427−0.601608hypothetical protein MGC4309
1500815−0.6008126Homo sapiens cDNA: FLJ22130 fis, clone HEP19632
416959−0.5999728nuclear factor I/B
782460−0.5988226Homo sapiens cDNA FLJ10500 fis, clone NT2RP2000369
824602−0.5965816interferon, gamma-inducible protein 16
878798−0.5956349beta-2-microglobulin
32299−0.5945347inositol(myo)-1(or 4)-monophosphatase 2
277571−0.59335KIAA1706 protein
703964−0.5918604inositol polyphosphate phosphatase-like 1
796498−0.5900328hypothetical protein FLJ14007
504927−0.5894078epithelial protein up-regulated in carcinoma, membrane
associated protein 17
813520−0.5889772EphB3
341763−0.5868818caspase 5, apoptosis-related cysteine protease
1856063−0.5866417tweety (Drosophila) homolog 1
825356−0.5850287Homo sapiens cDNA FLJ11997 fis, clone HEMBB1001458
745011−0.5836864Homo sapiens mRNA for K1AA1750 protein, partial cds
812256−0.5832398Homo sapiens cDNA: FLJ21693 fis, clone COL09609
2017960−0.5822336PP1201 protein
713129−0.5822078granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated
serine esterase 3)
815142−0.5782827spastic ataxia of Charlevoix-Saguenay (sacsin)
80727−0.5766546receptor tyrosine kinase-like orphan receptor 1
45099−0.5753698regucalcin (senescence marker protein-30)
415816−0.5750184ESTs
306798−0.5742664NGFI-A binding protein 1 (EGR1 binding protein 1)
563634−0.5737239dendritic cell protein
757191−0.5735888ESTs
740620−0.5730915tropomyosin 2 (beta)
1470048−0.5729078lymphocyte antigen 6 complex, locus E
788518−0.5724497peroxisomal membrane protein 3 (35 kD, Zellweger syndrome)
177665−0.5718286
221846−0.5714352checkpoint suppressor 1
37671−0.5712258hypothetical protein FLJ21610
2072862−0.5694163v-akt murine thymoma viral oncogene homolog 2
132637−0.5694152grancalcin, EF-hand calcium-binding protein
1474900−0.5693091keratin 15
34093−0.5692081EST
1881689−0.568518hypothetical protein FLJ20281
1883327−0.5684295ESTs
1902764−0.56830526-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
781017−0.5676944early growth response 2 (Krox-20 (Drosophila) homolog)
34150−0.5673709ESTs
137602−0.5667198Homo sapiens mRNA; cDNA DKFZp434G0972 (from clone
DKFZp434G0972)
460126−0.5652362KIAA0819 protein
470128−0.5652125myosin IE
1880885−0.5638973ESTs
811848−0.5637994hypothetical protein
1417886−0.563564hypothetical protein FLJ23239
756847−0.5632582deformed epidermal autoregulatory factor 1 (Drosophila)
505243−0.5616577inositol 1,4,5-triphosphate receptor, type 2
840677−0.5611985immunoglobulin kappa constant
1911663−0.5610314ESTs
66491−0.5579872plasmolipin
1420842−0.5574834mucosa associated lymphoid tissue lymphoma translocation
gene 1
1626996−0.5573507c-fos induced growth factor (vascular endothelial growth factor
D)
281190−0.5572026ESTs
796475−0.5563457four and a half LIM domains 3
1870305−0.5558155heat shock 27 kD protein 2
767456−0.5555493hypothetical protein FLJ22167
292388−0.554297
795178−0.5532479lactate dehydrogenase C
809998−0.5519459amylase, alpha 2A; pancreatic
1734754−0.5512835hypothetical protein B
27769−0.5501268ESTs
950355−0.550114ESTs, Weakly similar to S13495 pregnancy zone protein
[H. sapiens]
144924−0.5498123chromosome 6 open reading frame 5
502436−0.5489864insulin receptor substrate 2
138672−0.5483104ESTs
160192−0.5482009ESTs, Weakly similar to 2004399A chromosomal protein
[H. sapiens]
768007−0.547374hypothetical protein
130201−0.5468031intercellular adhesion molecule 2
1587847−0.5466442minichromosome maintenance deficient (mis5, S. pombe) 6
1558212−0.5458261ESTs
1470278−0.5449844hypothetical protein FLJ21841
811088−0.5445374ephrin-B3
197056−0.5440514ESTs
868630−0.5426831transforming growth factor beta-stimulated protein TSC-22
530958−0.5423532smoothened (Drosophila) homolog
810097−0.5423072Homo sapiens cDNA: FLJ21721 fis, clone COLF0381
207735−0.5422739serine (or cysteine) proteinase inhibitor, clade A (alpha-1
antiproteinase, antitrypsin), member 1
79254−0.541673MHC class I region ORF
85634−0.5410684complement component 1, s subcomponent
531319−0.5404527serine/threonine kinase 12
754028−0.5396001KIAA0469 gene product
470148−0.5393193ESTs
35828−0.5391332diphtheria toxin receptor (heparin-binding epidermal growth
factor-like growth factor)
970590−0.5383645Homo sapiens mRNA; cDNA DKFZp434A115 (from clone
DKFZp434A115)
753162−0.5377767KIAA0603 gene product
241066−0.5369493Homo sapiens cDNA: FLJ21028 fis, clone CAE07155
1909935−0.5368632ESTs
153760−0.5361176EphB1
247616−0.5358709lipoma HMGIC fusion partner
1031640−0.5354869ESTs
24958−0.5349519Homo sapiens mRNA; cDNA DKFZp434C2016 (from clone
DKFZp434C2016)
1686766−0.5342051Rag D protein
2009491−0.5334821cyclin-E binding protein 1
611532−0.5320107troponin I, skeletal, fast
811149−0.5319742chromosome 9 open reading frame 3
844703−0.5309356Sam68-like phosphotyrosine protein, T-STAR
70749−0.5295091Homo sapiens cDNA: FLJ21874 fis, clone HEP02488
324927−0.5284875KIAA0375 gene product
491519−0.5282605Homo sapiens clone 24775 mRNA sequence
754040−0.5282144NS1-associated protein 1
767765−0.5282008GTP-binding protein overexpressed in skeletal muscle
2028722−0.5277252osteoblast specific factor 2 (fasciclin I-like)
509458−0.5266016hypothetical protein from clone 643
360518−0.5253745guanine nucleotide binding protein (G protein), gamma
transducing activity polypeptide 1
40021−0.5240809hairless protein (putative single zinc finger transcription factor
protein, responsible for autosomal recessive universal
congenital alopecia, HR gene)
1901310−0.5229967KIAA1209 protein
33949−0.5222135phosphoribosyl pyrophosphate synthetase-associated protein 1
814316−0.521831ribosomal protein L13
488130−0.5201845Homo sapiens cDNA FLJ20767 fis, clone COL06986
462939−0.5200097ESTs
770848−0.5188717ESTs, Weakly similar to ALU1_HUMAN ALU SUBFAMILY J
SEQUENCE CONTAMINATION WARNING ENTRY [H. sapiens]
245296−0.5184238RAD52 (S. cerevisiae) homolog
753034−0.5182019nuclear factor I/X (CCAAT-binding transcription factor)
712829−0.5181236LIM domain only 2 (rhombotin-like 1)
1577920−0.5161377ESTs
814526−0.5156162seb4D
773301−0.5147295cadherin 3, type 1, P-cadherin (placental)
77972−0.5144916complement component 3
1704155−0.5133015hypothetical protein SBBI48
1572298−0.5126426CD3Z antigen, zeta polypeptide (TiT3 complex)
755689−0.5122586retinoic acid receptor, gamma
1876217−0.5108977DnaJ (Hsp40) homolog, subfamily A, member 2
712401−0.5107426phosphoinositide-3-kinase, catalytic, delta polypeptide
713839−0.5088558transcription factor AP-4 (activating enhancer-binding protein 4)
180803−0.5083354inositol polyphosphate-1-phosphatase
814443−0.5075293hypothetical protein MGC3232
|
Example V
[0106] Genes for Discriminating Between Normal and DCIS
[0107] As shown in Table 4 below, 1300 genes were identified as being able to discriminate between normal and DCIS.
4TABLE 4
|
|
CloneIDWeightDescription
|
|
13234481.397391cysteine-rich protein 1 (intestinal)
7886541.2615322growth factor receptor-bound protein 2
15050381.1904802hypothetical protein FLJ20171
7456061.1825585hypothetical protein PP591
5653191.0969429Homo sapiens mRNA; cDNA DKFZp564B1264 (from clone
DKFZp564B1264)
351471.0886084ESTs, Weakly similar to unnamed protein product [H. sapiens]
1788051.0274897Human DNA sequence from clone RP5-850E9 on chromosome
20. Contains part of the gene for a novel C2H2 type zinc finger
protein similar to Drosophila Scratch (Scrt), Slug and Xenopus
Snail, a novel gene similar to Drosophila CG6762, STSs, GSSs
and five CpG
7964691.0269115HSPC150 protein similar to ubiquitin-conjugating enzyme
16098361.0164283glutamate-ammonia ligase (glutamine synthase)
3661321.013995succinate dehydrogenase complex, subunit C, integral
membrane protein, 15 kD
15000001.0118809H2B histone family, member B
8122381.0043787hypothetical protein MGC4692
5950370.990461retinoic acid induced 3
15545490.9764206hydroxyacyl glutathione hydrolase
4889640.9569176H2A histone family, member O
1381890.9386802Wolfram syndrome 1 (wolframin)
2839190.937383H2A histone family, member L
8235980.9153521proteasome (prosome, macropain) 26S subunit, non-ATPase,
12
8107110.9128832stearoyl-CoA desaturase (delta-9-desaturase)
19113430.9116745RAB26, member RAS oncogene family
5032150.9108688pilin-like transcription factor
8117740.9073627CGI-49 protein
1220770.9021901putative membrane protein
14922380.9019233HSPC003 protein
4696860.8919992Ric (Drosophila)-like, expressed in many tissues
6855160.8762873putative G protein-coupled receptor
3598870.8692353translocase of inner mitochondrial membrane 17 (yeast)
homolog A
1310940.8675719Homo sapiens cDNA: FLJ21587 fis, clone COL06946
14924630.8634521selenoprotein X, 1
8431950.8603524phosphoserine phosphatase
7967230.8573155Homo sapiens clone CDABP0014 mRNA sequence
4882020.8463961ESTs, Weakly similar to YZ28_HUMAN HYPOTHETICAL
PROTEIN ZAP128 [H. sapiens]
14924260.8445255chromosome 19 open reading frame 3
2803750.8392836PRO2000 protein
2908410.8346933H2B histone family, member A
19179410.8340565purine-rich element binding protein B
14694250.8296141SRY (sex determining region Y)-box 22
20169080.8282806ESTs, Weakly similar to CA13_HUMAN COLLAGEN ALPHA
1(III) CHAIN PRECURSOR [H. sapiens]
4715680.8264546hematological and neurological expressed 1
1500030.8251408hypothetical protein FLJ13187
20291730.8213721ESTs, Weakly similar to N-WASP [H. sapiens]
7310440.8169506glutaredoxin 2
2448010.8066357Rho guanine exchange factor (GEF) 11
20546350.7914755proteasome (prosome, macropain) subunit, alpha type, 7
16054260.787298hypothetical protein FLJ13352
17097910.7837549BAI1-associated protein 1
4700610.77977seven in absentia (Drosophila) homolog 2
16408210.7790286ESTs, Weakly similar to I78885 serine/threonine-specific
protein kinase [H. sapiens]
1994030.7754748lectin, galactoside-binding, soluble, 8 (galectin 8)
7414740.7732047glucose phosphate isomerase
14358620.7697423antigen identified by monoclonal antibodies 12E7, F21 and O13
2395680.767277annexin A9
8416790.7666686calcium and integrin binding protein (DNA-dependent protein
kinase interacting protein)
7299750.7649717meningioma expressed antigen 5 (hyaluronidase)
20166480.7633048Homo sapiens mRNA; cDNA DKFZp434N1728 (from clone
DKFZp434N1728)
20521130.7627076hypothetical protein FLJ10903
3473730.7563599transcription elongation factor B (SIII), polypeptide 1 (15 kD,
elongin C)
8140540.7539407KIAA0040 gene product
7419770.7538359B-factor, properdin
2725290.7486269phosphomannomutase 2
8248790.7432221hypothetical protein MGC11275
1222410.7394284proteasome (prosome, macropain) subunit, beta type, 2
7444170.7356767carnitine acetyltransferase
8681280.7347171JM4 protein
2413480.7324115prenylcysteine lyase
5098230.7304507carcinoembryonic antigen-related cell adhesion molecule 6
(non-specific cross reacting antigen)
16360920.7270017hypothetical protein FLJ20657
424080.725109hypothetical protein MGC4604
6865520.7231319golgi phosphoprotein 1
8980320.7216KIAA0097 gene product
7546280.7195265ESTs
8262560.7190206transmembrane 7 superfamily member 1 (upregulated in
kidney)
20431670.7167431BCL2-associated athanogene 3
8271710.7154964ESTs
5027740.7148695hypothetical protein FLJ20623
7810970.7126945reticulon 3
8107250.7115051ATPase, H+ transporting, lysosomal (vacuolar proton pump)
21 kD
7685700.7102463hypothetical protein FLJ11280
8132810.7092607WW domain-containing protein 1
398840.7075527IMP (inosine monophosphate) dehydrogenase 1
9698770.707469synaptosomal-associated protein, 25 kD
7042540.7061648a disintegrin and metalloproteinase domain 8
2360340.7039329uncoupling protein 2 (mitochondrial, proton carrier)
7824280.701077KIAA0250 gene product
4893510.7003616hypothetical protein DKFZp566J2046
377080.6986007hypothetical protein MGC3101
2714720.6978431C3HC4-like zinc finger protein
7826080.6978285mitochondrial ribosomal protein L9
7044140.6940045small nuclear ribonucleoprotein polypeptides B and B1
2889990.6938843small protein effector 1 of Cdc42
517730.6925666hypothetical protein MGC3077
1244470.6918546KIAA1184 protein
4700990.6915952HT002 protein; hypertension-related calcium-regulated gene
4297990.6915155hypothetical protein FLJ21939 similar to 5-azacytidine induced
gene 2
18588920.6904662hypothetical protein MGC4825
7966940.6893307baculoviral IAP repeat-containing 5 (survivin)
2842610.6890531uncharacterized hematopoietic stem/progenitor cells protein
MDS030
8145280.6878182Homo sapiens cDNA: FLJ22139 fis, clone HEP20959
7886540.6866898
686360.6838604hypothetical protein MGC2477
3084660.6800103GTP-binding protein Sara
15740580.67963571-acylglycerol-3-phosphate O-acyltransferase 2
(lysophosphatidic acid acyltransferase, beta)
795200.6769129RAB2, member RAS oncogene family
14732890.674396protective protein for beta-galactosidase (galactosialidosis)
3657380.6740773ESTs
7680640.673441cytochrome P450, subfamily I (aromatic compound-inducible),
polypeptide 1
7739220.6730903KIAA0005 gene product
18692010.6727191hypothetical protein MGC2745
6861720.6724684KIAA0008 gene product
8105580.6698194proteasome (prosome, macropain) 26S subunit, ATPase, 4
7532990.6694573hypothetical protein FLJ10504
7254540.6669973CDC28 protein kinase 2
4701240.6641768RAD1 (S. pombe) homolog
20140340.6620303methylene tetrahydrofolate dehydrogenase (NAD+ dependent),
methenyltetrahydrofolate cyclohydrolase
2090660.6611038
8597610.6573769poliovirus receptor-related 2 (herpesvirus entry mediator B)
5892320.6551447hypothetical protein FLJ11506
3405580.6550804actin related protein 2/3 complex, subunit 5 (16 kD)
1867680.6550741Homo sapiens, clone IMAGE: 3604869, mRNA
8136290.6549767YME1 (S. cerevisiae)-like 1
14927800.6544659Homo sapiens cDNA FLJ14459 fis, clone HEMBB1002409
8099440.6530236KIAA0310 gene product
833630.6526164protein-L-isoaspartate (D-aspartate) O-methyltransferase
1493550.6520333translocating chain-associating membrane protein
7526310.650399fibroblast growth factor receptor 3 (achondroplasia,
thanatophoric dwarfism)
7856160.6484744signal sequence receptor, alpha (translocon-associated protein
alpha)
766050.6484522nesca protein
7427070.6476434ESTs, Weakly similar to MUC2_HUMAN MUCIN 2
PRECURSOR [H. sapiens]
3256060.647638hypothetical protein MGC14353
7699210.6464245ubiquitin carrier protein E2-C
14350030.6457486tumor suppressing subtransferable candidate 1
14739220.6450042actin related protein 2/3 complex, subunit 3 (21 kD)
14563480.6417276N-acetylneuraminic acid phosphate synthase; sialic acid
synthase
15001620.6416738ESTs
15732510.6410435peroxisomal long-chain acyl-coA thioesterase
462480.640944ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
493510.6386132SEX gene
8240520.6375787chromosome 6 open reading frame 1
14203700.6344209biliverdin reductase B (flavin reductase (NADPH))
8429940.6343858cathepsin Z
8106120.6341098S100 calcium-binding protein A11 (calgizzarin)
1236140.633308hypothetical protein MGC4675
1247810.6324418squalene epoxidase
8143780.6317382serine protease inhibitor, Kunitz type, 2
21080770.6312583CGI-112 protein
2899780.6308861ubiquitin-like 4
677650.6300886carboxypeptidase M
7425950.6265846cyclin-dependent kinase 5
7270780.6263882Homo sapiens cDNA: FLJ23602 fis, clone LNG15735
4877330.6262778
3660670.625758cerebellar degeneration-related protein (62 kD)
2927700.6231261Homo sapiens, clone IMAGE: 3627860, mRNA, partial cds
3663530.6203012DKFZP564C186 protein
8100630.620086growth factor, erv1 (S. cerevisiae)-like (augmenter of liver
regeneration)
8101240.6162712platelet-activating factor acetylhydrolase, isoform Ib, gamma
subunit (29 kD)
7137820.6149877a disintegrin and metalloproteinase domain 15 (metargidin)
7826080.6137782
5301970.6122721ESTs, Moderately similar to ALU8_HUMAN ALU SUBFAMILY
SX SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
442920.6122043Homo sapiens mRNA; cDNA DKFZp434C107 (from clone
DKFZp434C107)
20180840.6119069Ste-20 related kinase
7890120.6104002fibulin 2
2566190.6102239hydroxysteroid (17-beta) dehydrogenase 7
8253270.6094796Homo sapiens cDNA FLJ14105 fis, clone MAMMA1001202
8429800.6081528developmentally regulated GTP-binding protein 1
8117610.6045809Nijmegen breakage syndrome 1 (nibrin)
16019470.6045149cytochrome c oxidase subunit VIIa polypeptide 2 (liver)
14749550.6045103TATA box binding protein (TBP)-associated factor, RNA
polymerase II, N, 68 kD (RNA-binding protein 56)
17585900.6040962fatty-acid-Coenzyme A ligase, long-chain 3
14567010.6038789B-cell CLL/lymphoma 9
2072880.603346insulin induced gene 1
16316990.6027911valosin-containing protein
16356810.6022549NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2
(8 kD, B8)
7684520.6018854Homo sapiens EST from clone 491476, full insert
8978060.6015789hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix
transcription factor)
1084250.6008772
7707850.6003111,2-alpha-mannosidase IC
21105110.5987863artemin
7857950.598504hypothetical protein FLJ12910
1202710.5982507hypothetical protein MGC4692
2486490.5975539hypothetical protein FLJ13910
22441960.5972111B-cell receptor-associated protein BAP29
664060.5966701hypothetical protein DKFZp762E1312
15370010.59528ESTs
20289490.5946445hypothetical protein PRO1855
401730.5940582KIAA0807 protein
4907780.5932928low molecular mass ubiquinone-binding protein (9.5 kD)
5038890.5930018hypothetical protein FLJ10656
7810190.5918462paraoxonase 2
1961890.5916252cytochrome b-5
17343090.5894492sperm associated antigen 4
8143500.588877insulin-degrading enzyme
20188210.5887114ATPase inhibitor precursor
3653580.5883524pM5 protein
4302350.5874504H2B histone family, member Q
14223380.5874097ribonucleotide reductase M2 polypeptide
7708450.5870877hexokinase 1
1725170.5869599hippocalcin-like 1
18844040.5849803KIAA0285 gene product
6259230.5839066phosphoenolpyruvate carboxykinase 2 (mitochondrial)
23223670.5832711reticulon 4
6246670.5828923CGI-92 protein
8263630.5823873lysophospholipase II
8592280.5817344isocitrate dehydrogenase 1 (NADP+), soluble
23020990.5813788sialidase 3 (membrane sialidase)
2090660.5794211serine/threonine kinase 15
18447650.57783Homo sapiens mRNA; cDNA DKFZp564O1763 (from clone
DKFZp564O1763)
1919040.5770884BUB3 (budding uninhibited by benzimidazoles 3, yeast)
homolog
9512330.5767874proteasome (prosome, macropain) subunit, beta type, 3
8093570.5767382Bernardinelli-Seip congenital lipodystrophy 2 (seipin)
8257400.5762093DKFZp434J1813 protein
515320.5758765ADP-ribosylation factor-like 6 interacting protein
2030030.5743416non-metastatic cells 4, protein expressed in
20155170.5742448hypothetical protein FLJ22237
2050490.5734444protein kinase H11; small stress protein-like protein HSP22
7526430.5732323group XII secreted phospholipase A2
7534000.5732322CGI-204 protein
5648470.573202ESTs
7857660.5731938hypothetical protein
8255850.5722829tubulin-specific chaperone e
375540.5706008hypothetical protein FLJ22353
1448800.5704416hypothetical protein from EUROIMAGE 1759349
492730.5691181solute carrier family 27 (fatty acid transporter), member 4
7435890.568516ESTs, Weakly similar to T2D3_HUMAN TRANSCRIPTION
INITIATION FACTOR TFIID 135 KDA SUBUNIT [H. sapiens]
16162530.567837breast carcinoma amplified sequence 1
15819410.5676045hypothetical protein FLJ14540
4315050.5670119ESTs, Highly similar to A31026 probable membrane receptor
protein [H. sapiens]
20192230.5663073mitochondrial ribosomal protein L17
8408780.5648009seladin-1
8110240.5646461bone marrow stromal cell antigen 2
5644920.5626905mitochondrial carrier homolog 2
7258410.561197KIAA0662 gene product
7017510.561139cut (Drosophila)-like 1 (CCAAT displacement protein)
7677980.560181ATX1 (antioxidant protein 1, yeast) homolog 1
1098630.559767epithelial membrane protein 2
15360060.5590182ESTs
18451690.5588842RAB35, member RAS oncogene family
3436070.5588222AD-015 protein
2125420.5587394Homo sapiens cDNA FLJ12900 fis, clone NT2RP2004321
17004360.5569949ESTs
7952560.5567908NPD007 protein
20115150.5566484DKFZP586B0923 protein
5043080.5564295hypothetical protein FLJ10540
7729250.5562164HSPCO34 protein
4693830.5558732chromosome 8 open reading frame 1
8104020.5555749hypothetical protein
14095090.554703troponin T1, skeletal, slow
20508270.5544641proteasome (prosome, macropain) 26S subunit, ATPase, 5
7703550.5518948lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
8134100.5513568polymerase (RNA) II (DNA directed) polypeptide K (7.0 kD)
807640.5510626hypothetical protein
16317350.5502891Homo sapiens, clone IMAGE: 3604336, mRNA, partial cds
6252340.550211KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein
retention receptor 3
2799700.5502099adenosine A2a receptor
15184020.5497179KIAA1361 protein
7533780.548977hypothetical protein FLJ22649 similar to signal peptidase
SPC22/23
5945000.5474088EST
5907740.5460342mitogen-activated protein kinase 13
20558070.5460123protein kinase domains containing protein similar to
phosphoprotein C8FW
7731880.5454398nuclear receptor subfamily 1, group D, member 2
1268510.5454014hypothetical protein FLJ11160
2447640.5450377B7 homolog 3
8134190.5447006hydroxyacyl-Coenzyme A dehydrogenase, type II
2333490.5446353hypothetical protein FLJ10761
7857010.5446211RAB31, member RAS oncogene family
2689460.5440003WD40 protein Ciao1
6114430.5432426myoglobin
5105750.5430211hypothetical protein FLJ22087
4914650.5420241hypothetical protein FLJ10035
13930180.5404243general transcription factor IIIC, polypeptide 1 (alpha subunit,
220 kD)
7858400.5401209SEC24 (S. cerevisiae) related gene family, member D
14601100.5392177proteasome (prosome, macropain) subunit, beta type, 5
7313080.5371204citrate synthase
3011220.5368073extracellular matrix protein 1
665350.5363248ornithine decarboxylase antizyme 2
2651030.5361177Homo sapiens mRNA; cDNA DKFZp547M123 (from clone
DKFZp547M123)
7883340.5347204mitochondrial ribosomal protein L23
8136310.534683seven transmembrane protein TM7SF3
15533060.5344506proteasome (prosome, macropain) 26S subunit, non-ATPase,
11
14614770.5343834Homo sapiens mRNA; cDNA DKFZp586I0324 (from clone
DKFZp586I0324)
1996450.5334951nicastrin
8844250.5327862chaperonin containing TCP1, subunit 5 (epsilon)
15188900.5314577metallothionein-like 5, testis-specific (tesmin)
7564420.5313744P450 (cytochrome) oxidoreductase
23260570.5310995MLN51 protein
1387880.5302936prolactin receptor
16980360.5298397ubiquitin-conjugating enzyme E2 variant 1
8115850.5288102huntingtin (Huntington disease)
7951850.5276861xenotropic and polytropic retrovirus receptor
6299440.5273447myosin VB
7841050.5259815ESTs
16967570.5254296hypothetical protein KIAA1165
1398350.5253386UDP-glucose dehydrogenase
17382080.5240868a disintegrin-like and metalloprotease (reprolysin type) with
thrombospondin type 1 motif, 4
5934310.5235073ESTs, Moderately similar to CEGT_HUMAN CERAMIDE
GLUCOSYLTRANSFERASE [H. sapiens]
23090730.5226599frizzled (Drosophila) homolog 5
8254700.5218731topoisomerase (DNA) II alpha (170 kD)
8396820.5208954ubiquitin-conjugating enzyme E2N (homologous to yeast
UBC13)
15171710.5195255interleukin 2 receptor, alpha
3765160.5195127cell division cycle 4-like
7744460.5181672adrenomedullin
14760530.5179015RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
4895940.5178551hypothetical protein FLJ11565
15739460.5173691programmed cell death 9
5038510.5170168nuclear receptor co-repressor/HDAC3 complex subunit
8824840.5165756chaperonin containing TCP1, subunit 7 (eta)
510830.5162686catenin (cadherin-associated protein), delta 2 (neural
plakophilin-related arm-repeat protein)
2212950.5157877inhibitor of DNA binding 2, dominant negative helix-loop-helix
protein
7259780.5147267ESTs, Moderately similar to ALU7_HUMAN ALU SUBFAMILY
SQ SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
7967570.5122331adaptor-related protein complex 3, sigma 1 subunit
1503140.5114988lysophospholipase I
3020310.5111067Ste20-related serine/threonine kinase
2935690.5103638chromosome 1 open reading frame 21
8383660.50726233-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
(hydroxymethylglutaricaciduria)
1221470.5067128
1207490.5060444ESTs, Moderately similar to KIAA1215 protein [H. sapiens]
8555630.5059683v-erb-b2 avian erythroblastic leukemia viral oncogene homolog
3
4877330.5057167NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2
(8 kD, AGGG)
6256930.5055986hypothetical protein MGC10911
8137510.5051993sialyltransferase 4C (beta-galactosidase alpha-2,3-
sialytransferase)
8428250.5047729G1 to S phase transition 1
3236930.5047237adaptor-related protein complex 1, sigma 1 subunit
2025140.5045411DNA (cytosine-5-)-methyltransferase 3 alpha
15177490.5045134ESTs
1243310.5025464cleavage and polyadenylation specific factor 5, 25 kD subunit
14744240.5021308Homo sapiens cDNA FLJ12758 fis, clone NT2RP2001328
18489770.5015371glycerol kinase
4548960.5013449DnaJ (Hsp40) homolog, subfamily A, member 2
17027420.5011725solute carrier family 7 (cationic amino acid transporter, y+
system), member 5
7461630.5009715ESTs, Weakly similar to ALU1_HUMAN ALU SUBFAMILY J
SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
8107340.4998276polymerase (DNA-directed), delta 4
7252840.4992842phosphorylase kinase, gamma 2 (testis)
521030.4988158hypothetical protein FLJ23045
7391090.4986821adaptor-related protein complex 2, sigma 1 subunit
4885050.4981852accessory proteins BAP31/BAP29
3650600.4976091RAB11A, member RAS oncogene family
415690.4974126hypothetical protein FLJ12650
813360.4955353uteroglobin
7007920.4949149cyclin-dependent kinase inhibitor 3 (CDK2-associated dual
specificity phosphatase)
15927150.4944665Homer, neuronal immediate early gene, 3
1425860.4942961MCT-1 protein
2614720.4941066putative nuclear protein ORF1-FL49
2468000.4927231hypothetical protein FLJ10803
1242980.4922937microsomal glutathione S-transferase 3
18986190.4916939hypothetical protein MGC15737
7391260.4905859tissue specific transplantation antigen P35B
3768750.490328flavin containing monooxygenase 1
3581620.4901786protein predicted by clone 23627
5907590.4895021sterol-C4-methyl oxidase-like
19476470.4887766CGI-147 protein
1202710.4886584hypothetical protein MGC4692
4886420.4881613ESTs, Weakly similar to I38022 hypothetical protein [H. sapiens]
14924680.4878466DEME-6 protein
3455380.4875796cathepsin L
3440910.4871044ESTs
1234410.4862068ribosomal protein L7a
2029010.48608vav 2 oncogene
7574890.4859039tubulin, alpha 2
491170.4856399KIAA0215 gene product
8129940.4854155retinoid X receptor, alpha
7310230.4853217WD repeat domain 5
8148990.4840969BCL2/adenovirus E1B 19 kD-interacting protein 3-like
7566660.4835569protein phosphatase 1, catalytic subunit, alpha isoform
20183370.4833207glucosidase, beta; acid (includes glucosylceramidase)
15688250.4829928Arg/Abl-interacting protein ArgBP2
8693750.4827093isocitrate dehydrogenase 2 (NADP+), mitochondrial
5952130.4824381hypothetical protein
7443740.4824256putative ankyrin-repeat containing protein
730090.4822587ESTs, Weakly similar to A43932 mucin 2 precursor, intestinal
[H. sapiens]
706060.481356ESTs
3584560.4812268Sec61 gamma
8107620.4806851SNARE protein
8239300.4800704actin related protein 2/3 complex, subunit 1A (41 kD)
20208980.4797032procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
5649810.4782207ESTs
16372820.4777562hexokinase 2
8557490.476968triosephosphate isomerase 1
7449440.4769116myosin VI
2637270.4767486DNA segment, single copy probe LNS-CAI/LNS-CAII (deleted
in polyposis
4700920.476018like-glycosyltransferase
7825130.4759571interferon, alpha-inducible protein (clone IFI-6-16)
8978130.4749348polyadenylate binding protein-interacting protein 1
1418520.4748862purinergic receptor P2Y, G-protein coupled, 2
8558000.4748367prolyl endopeptidase
413560.4746307protein phosphatase 2, regulatory subunit B (B56), alpha
isoform
7533200.4744893hypothetical protein FLJ20533
7841400.474314WD repeat domain 15
5303100.4739401KIAA0143 protein
8133870.4727653diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
3582670.4719017EST, Moderately similar to AF119917 63 PRO2831 [H. sapiens]
20185270.4717524dolichyl-phosphate mannosyltransferase polypeptide 3
7813420.4716127hypothetical protein MGC11115
7857070.4708376protein regulator of cytokinesis 1
1406350.4706227ESTs
8143060.4705205tumor protein D52
7841500.4702303RAB31, member RAS oncogene family
8397460.4699443Homo sapiens, Similar to RIKEN cDNA 5830420C20 gene,
clone IMAGE: 3633379, mRNA, partial cds
3773840.4698441nuclear receptor subfamily 2, group F, member 2
1832000.4697062fumarylacetoacetate hydrolase (fumarylacetoacetase)
3454230.4694596DKFZP564M112 protein
2046860.4687565FXYD domain-containing ion transport regulator 1
(phospholemman)
4285820.4684705hypothetical protein FLJ20296
2108620.4683996acyl-Coenzyme A oxidase 1, palmitoyl
517410.4678807GTP-binding protein
2967020.4670993deiodinase, iodothyronine, type I
8408650.4666048myristoylated alanine-rich protein kinase C substrate
(MARCKS, 80K-L)
2785310.4663008cytochrome c oxidase subunit Vlc
605650.4659865lethal giant larvae (Drosophila) homolog 2
16395310.4658349RAB27A, member RAS oncogene family
3469420.4657601phosphatidylinositol glycan, class Q
8844980.4648731uncharacterized hypothalamus protein HT012
3457870.4647372highly expressed in cancer, rich in leucine heptad repeats
8252960.4646099low density lipoprotein receptor defect C complementing
22484880.4645687ems1 sequence (mammary tumor and squamous cell
carcinoma-associated (p80/85 src substrate)
8249620.4638758karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
8430540.4637278KIAA1533 protein
1402890.4624364hypothetical protein
15582330.4622449ESTs
2655920.4615543activated RNA polymerase II transcription cofactor 4
7677610.4608709DKFZP434B168 protein
7707660.4603527DKFZP564C1940 protein
8142090.4602081ESTs
7736740.4601667oncogene TC21
7037070.4599434aspartate beta-hydroxylase
9512160.4594172NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10
(22 kD, PDSW)
1393540.4582974hypothetical protein
797100.4579008KIAA0174 gene product
8235740.457224endosulfine alpha
8453630.4570873non-metastatic cells 1, protein (NM23A) expressed in
2837510.4567022cortistatin
8101560.4561539deoxythymidylate kinase (thymidylate kinase)
14167820.4558415creatine kinase, brain
8106090.4551167hypothetical protein PP1226
7706750.454729Homo sapiens cDNA: FLJ21323 fis, clone COL02374
6263180.4529715ubinuclein 1
383560.4513099cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
7050640.4497555transforming, acidic coiled-coil containing protein 3
1276460.4493747
2998150.4482635hypothetical protein DC42
8150260.4472842cleavage stimulation factor, 3′ pre-RNA, subunit 3, 77 kD
4898230.4468978COX17 (yeast) homolog, cytochrome c oxidase assembly
protein
4691510.4462659eukaryotic translation initiation factor 2, subunit 2 (beta, 38 kD )
418260.4456463ESTs
478530.445149aldehyde dehydrogenase 4 family, member A1
1447400.4449229similar to phosphatidylcholine transfer protein 2
7266580.4446657non-metastatic cells 3, protein expressed in
3255150.444486hypothetical protein FLJ10980
8157810.4441245heat shock 105 kD
7461900.4430243hypothetical protein DKFZp761B1514
20161940.4417536glia maturation factor, beta
15842870.4413331KIAA0241 protein
1478340.4412795zinc finger protein 217
1546100.4410751dynactin 4
7546530.4405873cadherin, EGF LAG seven-pass G-type receptor 3, flamingo
(Drosophila) homolog
7425810.440367Homo sapiens cDNA FLJ10366 fis, clone NT2RM2001420
23069870.4402912secreted and transmembrane 1
8784060.4396935metaxin 1
4306140.4388092,3-bisphosphoglycerate mutase
4151020.4387892cell division cycle 25C
3582170.4385343glypican 4
16366690.4384707hypothetical protein FLJ21939 similar to 5-azacytidine induced
gene 2
833580.4380365ESTs
13258160.4379227polymerase (RNA) II (DNA directed) polypeptide L (7.6 kD)
8245240.4375695UDP-galactose transporter related
7958050.4370764KIAA0332 protein
7439770.4368155Homo sapiens mRNA for TL132
7857930.4367093capping protein (actin filament) muscle Z-line, alpha 1
2662180.4362517hypothetical protein FLJ11350
7696000.4362014uracil-DNA glycosylase 2
16560620.4355127coagulation factor XII (Hageman factor)
20174150.43546centromere protein A (17 kD)
7722200.4352917for protein disulfide isomerase-related
8119070.4340842hypothetical protein FLJ22056
7893760.4339171thioredoxin reductase 1
7825030.4334388fatty acid desaturase 1
7538970.4324066autocrine motility factor receptor
7672890.4323485hypothetical protein FLJ10055
11599630.4323117interferon regulatory factor 7
16523100.4321289Homo sapiens, clone MGC: 19613 IMAGE: 3833049, mRNA,
complete cds
3589360.4314082ESTs, Weakly similar to T2D3_HUMAN TRANSCRIPTION
INITIATION FACTOR TFIID 135 KDA SUBUNIT [H. sapiens]
416980.4310061progesterone binding protein
16356650.430087Homo sapiens, RIKEN cDNA 2010100O12 gene, clone
MGC: 14813 IMAGE: 4133274, mRNA, complete cds
15586420.4298376hypothetical protein MGC2771
17332620.4289711BLu protein
8236940.4280476Homo sapiens chromosome 19, BAG CIT-HSPC_204F22
(BC228680), complete sequence; contains bacterial insertion
element
16418940.4277815EST
25721700.4277651ESTs, Weakly similar to T26581 hypothetical protein
Y32B12A.3 - Caenorhabditis elegans [C. elegans]
7310800.4276128hypothetical protein FLJ12661
6490840.4275738carbonic anhydrase XI
7954980.4270889putative transmembrane protein
8977700.4270685
22508390.4266248androgen receptor (dihydrotestosterone receptor; testicular
feminization; spinal and bulbar muscular atrophy; Kennedy
disease)
5107940.4264564c-myc binding protein
8258220.4255725hypothetical protein
8256590.424655N-myc downstream regulated
8260770.4242762pyruvate dehydrogenase (lipoamide) beta
2937270.4242722hypothetical protein MGC861
5038660.4230404sperm autoantigenic protein 17
4915270.4219324Homo sapiens, Similar to CG7083 gene product, clone
MGC: 10534 IMAGE: 3957147, mRNA, complete cds
2929360.4217115hypothetical protein FLJ10468
8239400.4214277transducer of ERBB2, 1
4149920.420994K562 cell-derived leucine-zipper-like protein 1
3461340.4209894calcium-regulated heat-stable protein (24 kD)
516570.4208387hypothetical protein ET
20097790.420743rabaptin-5
15232250.4195302oncostatin M receptor
8261940.4177687synaptotagmin-like 2
858040.4171445hypothetical protein FLJ21918
7252230.4167519KIAA0077 protein
797260.4162264ESTs, Highly similar to T46395 hypothetical protein
DKFZp434I1120.1 [H. sapiens]
17621110.4160469natriuretic peptide receptor C/guanylate cyclase C
(atrionatriuretic peptide receptor C)
5095880.4157059TATA box binding protein (TBP)-associated factor, RNA
polymerase II, J, 20 kD
3275060.4152106Homo sapiens mRNA full length insert cDNA clone
EUROIMAGE 327506
7440470.4151137polo (Drosophia)-like kinase
2214990.4149929KIAA0508 protein
7453940.4147627Homo sapiens cDNA: FLJ23249 fis, clone COL04196
1495390.4138942KIAA1700
8239070.4135955hypothetical protein FLJ10511
663170.4132405H1 histone family, member 2
2959860.413087emopamil-binding protein (sterol isomerase)
5652350.4124667spermine synthase
8253860.4121622ATP synthase, H+ transporting, mitochondrial F1F0, subunit d
2503130.4121296ESTs
720500.4114235chloride channel, nucleotide-sensitive, 1A
4178010.4112944mitochondrial ribosomal protein L27
8415010.4111707KIAA0102 gene product
8137070.410787regulator of G-protein signalling 16
7450830.4107735ubiquitin specific protease 18
2449740.4105439hypothetical protein FLJ22875
268830.4103824protein kinase (cAMP-dependent, catalytic) inhibitor beta
2802490.4096162Kruppel-like factor 7 (ubiquitous)
747380.409593Homo sapiens, clone IMAGE: 3535294, mRNA, partial cds
17534970.409432ovo (Drosophila) homolog-like 1
2042990.4073087replication protein A3 (14 kD)
3586090.4071867NADH dehydrogenase (ubiquinone) flavoprotein 3 (10 kD)
2901010.4071477ESTs
23062210.4068933wingless-type MMTV integration site family, member 10B
7264390.4067151CGI-143 protein
4318050.405447granulin
1113620.4045961
4721030.4043572soc-2 (suppressor of clear, C. elegans) homolog
7884440.4042598KIAA0033 protein
16322520.404193complement component 1, q subcomponent, alpha polypeptide
1501180.4039789hypothetical protein DKFZp434F054
5942260.403676Homo sapiens cDNA FLJ14459 fis, clone HEMBB1002409
14998280.4030396fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase,
Bombay phenotype included)
8109390.40256hypothetical protein FLJ22169
8416210.4020253ESTs, Weakly similar to JE0350 Anterior gradient-2 [H. sapiens]
2001440.4019827ring-box 1
4861100.4017126profilin 2
2922130.4014458guanine nucleotide binding protein (G protein), beta polypeptide
2
16311320.401216peptide transporter 3
6693790.4011648Homo sapiens BAC clone RP11-505D17 from 7p22-p21
7736850.4009493phosphodiesterase 4D interacting protein (myomegalin)
8104110.400096hypothetical protein FLJ13222
15831980.39981ESTs, Weakly similar to S65824 reverse transcriptase homolog
[H. sapiens]
842950.3989864interleukin 1 receptor antagonist
7573280.3985331hypothetical protein FLJ22678
356260.39822Homo sapiens cDNA FLJ14201 fis, clone NT2RP3002955
15878630.3980812acetyl-Coenzyme A acyltransferase 1 (peroxisomal 3-oxoacyl-
Coenzyme A thiolase)
7138620.3965573ubiquitin-protein isopeptide ligase (E3)
3437310.3962504
439770.3961848KIAA0182 protein
788690.3954821cell membrane glycoprotein, 110000M(r) (surface antigen)
7565950.395121S100 calcium-binding protein A10 (annexin II ligand, calpactin I,
light polypeptide (p11))
7011150.3936994hypothetical protein PRO2013
815990.3932769ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
16368440.3926235ring finger protein 14
7037390.392298nuclear cap binding protein subunit 1, 80 kD
2796330.3907503ESTs, Highly similar to I38759 zinc finger/leucine zipper protein
[H. sapiens]
1220910.3903482casein kinase 2, alpha 1 polypeptide
7860670.390151cell division cycle 25B
19095740.3900357mitochondrial ribosomal protein S11
2630130.3894536procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine
hydroxylase) 2
4910010.3884144glyoxalase I
4552750.3869874hypothetical protein FLJ23469
854500.3868399acyl-Coenzyme A oxidase 2, branched chain
7732860.3861543solute carrier family 9 (sodium/hydrogen exchanger), isoform 3
regulatory factor 1
3661560.3861394Homo sapiens cDNA FLJ14028 fis, clone HEMBA1003838
496300.3858335calcium channel, voltage-dependent, L type, alpha 1D subunit
1212510.385792hypothetical protein MGC5576
23222230.3853156small nuclear ribonucleoprotein polypeptide A
2769150.3851081DNA (cytosine-5-)-methyltransferase 3 beta
15912640.3849595transaldolase 1
14349480.3849593HIV TAT specific factor 1
4896570.3847527tryptophan rich basic protein
8109470.3833137LIS1-interacting protein NUDE1, rat homolog
2984170.3833014trefoil factor 3 (intestinal)
1547070.3831976MpV17 transgene, murine homolog, glomerulosclerosis
790320.3816428CGI-82 protein
19165750.3814206BCL2-interacting killer (apoptosis-inducing)
15854920.3812472ESTs
14553940.3811391cytochrome c
7699420.3810371kinesin-like 4
4293870.3810004chimerin (chimaerin) 2
7683770.3809674activity-dependent neuroprotective protein
2149960.380548ESTs
15576370.3805285ESTs
15175950.3804132KIAA0175 gene product
8412600.3801713hypothetical protein
4915240.3785047mitochondrial ribosomal protein L13
301700.3782337caspase 3, apoptosis-related cysteine protease
761960.3781541hypothetical protein FLJ20062
20177210.3781067ESTs, Weakly similar to 2109260A B cell growth factor
[H. sapiens]
1349180.3779855ESTs
3477260.377767homeo box D8
14691480.3776558FGFR1 oncogene partner
16493740.3771383homogentisate 1,2-dioxygenase (homogentisate oxidase)
2410430.3770901Human clone 137308 mRNA, partial cds
8158350.3764754hypothetical protein R33729_1
1434260.3764075ras homolog gene family, member B
14122450.3762847carboxypeptidase A2 (pancreatic)
7862650.3760397KIAA0750 gene product
2665000.3758559ESTs
7553010.3749416protein kinase C, delta
2943970.3744208DKFZP586A0522 protein
1558060.3741516
411230.3739475Homo sapiens, Similar to RIKEN cDNA 2210021G21 gene,
clone MGC: 14859 IMAGE: 3621871, mRNA, complete cds
4543390.3738929thiopurine S-methyltransferase
8103160.3732635very long-chain acyl-CoA synthetase; lipidosin
8121050.3731726ALL1-fused gene from chromosome 1q
7734260.3727398KIAA0391 gene product
15727100.3725734hypothetical protein FLJ21213
7728980.372064ribosomal protein S15a
2837390.3714571Homo sapiens cDNA FLJ14028 fis, clone HEMBA1003838
2895050.3714361ESTs, Moderately similar to S65657 alpha-1C-adrenergic
receptor splice form 2 [H. sapiens]
8971770.3712716phosphoglycerate mutase 1 (brain)
7825470.3708058ESTs
6478660.3706822Homo sapiens cDNA FLJ13975 fis, clone Y79AA1001585
16799420.3706798KIAA1053 protein
5664430.3705134Homo sapiens cDNA FLJ12793 fis, clone NT2RP2002033
3029960.3698028chloride intracellular channel 3
7266990.3695061ESTs, Weakly similar to AAB47496 NG5 [H. sapiens]
20942320.3693257chromosome 1 open reading frame 12
25496340.3692338activator of S phase kinase
1778270.3691459synaptotagmin VII
8528290.3691079karyopherin alpha 3 (importin alpha 4)
2077940.3679245nuclear factor (erythroid-derived 2), 45 kD
8239090.3675927
7438100.3667087hypothetical protein MGC2577
8100390.3662086defender against cell death 1
530390.3659853carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
8097270.365892unc-51 (C. elegans)-like kinase 1
261710.3656147KIAA0856 protein
15706630.3656056FK506-binding protein 4 (59 kD)
1792120.3649976ESTs, Moderately similar to T12539 hypothetical protein
DKFZp434J154.1 [H. sapiens]
2929960.36439tyrosine 3-monooxygenase/tryptophan 5-monooxygenase
activation protein, eta polypeptide
2599500.364359hypothetical protein FLJ14991
8157940.3643266nucleobindin 2
9652230.3642603thymidine kinase 1, soluble
5928010.3638731serine palmitoyltransferase, long chain base subunit 2
428310.3638165N-terminal kinase-like
7886410.3638059adaptor-related protein complex 1, sigma 2 subunit
8138450.3637519RNA, U transporter 1
520760.3633002neuroblastoma (nerve tissue) protein
1544930.3629466interferon-induced protein 75, 52 kD
15190130.3628651Homo sapiens, clone IMAGE: 3537447, mRNA, partial cds
14349050.3624537homeo box B7
8252820.3624112DKFZP586L0724 protein
3226170.3623149v-ral simian leukemia viral oncogene homolog B (ras related;
GTP binding protein)
8143530.3622592phorbol-12-myristate-13-acetate-induced protein 1
20179170.360911Homo sapiens cDNA: FLJ23371 fis, clone HEP16068, highly
similar to HSTFIISH Homo sapiens mRNA for transcription
elongation factor TFIIS
8136160.3608471FK506-binding protein like
18469820.3602851inhibin, beta C
5052890.3595549angiotensin II, type I receptor-associated protein
15650790.358728B-cell linker
7954010.3573481diacylglycerol O-acyltransferase (mouse) homolog
7810470.357207budding uninhibited by benzimidazoles 1 (yeast homolog)
3466960.3570344TEA domain family member 4
8094660.3568464DNA segment on chromosome 19 (unique) 1177 expressed
sequence
7432200.3566593hypothetical protein FLJ12517
1312680.3564734growth factor receptor-bound protein 14
1191330.3564449Homo sapiens mRNA; cDNA DKFZp434B231 (from clone
DKFZp434B231)
2427060.3562469Homo sapiens, Similar to RIKEN cDNA 5730494N06 gene,
clone MGC: 13349 IMAGE: 4249231, mRNA, complete cds
665990.3561699N-acetyltransferase 1 (arylamine N-acetyltransferase)
467160.3557556ESTs
2718990.355689Ser-Thr protein kinase related to the myotonic dystrophy protein
kinase
254400.3554359staufen (Drosophila, RNA-binding protein) homolog 2
18993120.3551595ESTs
7836810.354881upstream regulatory element binding protein 1
1310910.3548154Not56 (D. melanogaster)-like protein
3000990.3547857ESTs
5884360.3544892butyrate-induced transcript 1
20696020.3539588melanocortin 1 receptor (alpha melanocyte stimulating hormone
receptor)
8109590.3534647Rho GDP dissociation inhibitor (GDI) alpha
6499770.3534567Homo sapiens clone CDABP0014 mRNA sequence
3450690.3532976nuclear factor (erythroid-derived 2)-like 3
7674870.3530592ariadne (Drosophila) homolog, ubiquitin-conjugating enzyme
E2-binding protein, 1
8249430.3527414hypothetical protein
7449940.3526118hypothetical protein FLJ12242
7733810.3525581N-ethylmaleimide-sensitive factor attachment protein, alpha
15685610.3518952BCL2-like 1
20289160.3516982Homo sapiens mRNA for Hmob33 protein, 3′ untranslated
region
7534570.3515361NADH dehydrogenase (ubiquinone) Fe—S protein 1 (75 kD)
(NADH-coenzyme Q reductase)
21614270.3509243progesterone receptor
19473810.3509235hypothetical protein FLJ22329
824210.3506778zinc finger protein 6 (CMPX1)
5044610.3506753opsin 3 (encephalopsin)
7546250.3503938ATPase, Class II, type 9A
7955430.3500184thioredoxin peroxidase (antioxidant enzyme)
19103160.3498986KIAA0535 gene product
1727850.3493683NAG-5 protein
19100780.3491481ESTs, Weakly similar to YK54_YEAST HYPOTHETICAL 18.4
KD PROTEIN IN SIS2-MTD1 INTERGENIC REGION
[S. cerevisiae]
3213540.3489273mitochondrial ribosomal protein L15
8788150.3478601ADP-ribosylation factor 3
7417690.3475255polymerase (DNA directed), beta
4878310.3464379Homo sapiens cDNA FLJ14059 fis, clone HEMBB1000573
7540460.3464097DNA segment on chromosome X (unique) 9879 expressed
sequence
3449880.3464047
5300930.3463461myelin protein zero-like 1
8236150.3462131Homo sapiens cDNA: FLJ21245 fis, clone COL01184
1448810.3461767calumenin
1286950.3455381ESTs, Weakly similar to I38344 titin, cardiac muscle [H. sapiens]
8845110.3440809cytochrome c oxidase subunit VIIb
1287110.3437491anillin (Drosophila Scraps homolog), actin binding protein
4700350.3436575prokineticin 1 precursor
15681260.3434836solute carrier family 37 (glycerol-3-phosphate transporter),
member 1
5889110.34275632′,5′-oligoadenylate synthetase 1 (40-46 kD)
351910.3427094stromal cell-derived factor 2
8149890.3426862protein phosphatase 1G (formerly 2C), magnesium-dependent,
gamma isoform
736380.3425644protein tyrosine phosphatase type IVA, member 2
16051780.3416196amino acid transporter system A1
2140680.3414185GATA-binding protein 3
7555990.3413351interferon induced transmembrane protein 1 (9-27)
7824390.3413092ATP synthase, H+ transporting, mitochondrial FO complex,
subunit e
5950700.3408109stress-associated endoplasmic reticulum protein 1; ribosome
associated membrane protein 4
1834400.3406314arylsulfatase A
7745020.3402891protein tyrosine phosphatase, non-receptor type 12
2359860.338857wingless-type MMTV integration site family, member 11
2899360.3382708solute carrier family 7, (cationic amino acid transporter, y+
system) member 11
8241080.3381616SCAN domain-containing 1
266170.3371923activated leucocyte cell adhesion molecule
8104970.3367408ESTs, Weakly similar to A35363 synapsin I splice form a
[H. sapiens]
400420.3362557hypothetical protein FLJ10747
8111420.3361932phosphoinositide-3-kinase, regulatory subunit, polypeptide 2
(p85 beta)
20466790.3358043ESTs
4874440.3357395cyclic AMP phosphoprotein, 19 kD
787360.3354693Homo sapiens clone 24877 mRNA sequence
16373020.3353374DNAJ domain-containing
8256060.335057kinesin-like 1
763620.3349202spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
18830280.3345488Homo sapiens mRNA; cDNA DKFZp434J1912 (from clone
DKFZp434J1912)
7893830.3341667cAMP responsive element modulator
3025490.3340582basic transcription element binding protein 1
3092880.334016replication factor C (activator 1) 4 (37 kD)
7123140.3338146myosin regulatory light chain interacting protein
2416770.3332521Homo sapiens, clone MGC: 18110 IMAGE: 4152745, mRNA,
complete cds
8982530.3331924reticulocalbin 2, EF-hand calcium binding domain
7709920.3331315
812030.3328573paraoxonase 3
4164360.3325571mitochondrial ribosomal protein L24
16606660.332029carbonic anhydrase VB, mitochondrial
8115820.3319191golgi phosphoprotein 2
20541220.3318413solute carrier family 11 (proton-coupled divalent metal ion
transporters), member 3
8378640.3314764progestin induced protein
16322470.3312131hypothetical protein FLJ23436
2981430.3307258ESTs
20279520.3307056hypothetical protein FLJ20297
16002810.3304323nucleolar protein 3 (apoptosis represser with CARD domain)
8142700.3300091polymyositis/scleroderma autoantigen 1 (75 kD)
4155890.3296266Homo sapiens clone PP1498 unknown mRNA
19129510.329484uterine-derived 14 kDa protein
7266370.3292392t-complex-associated-testis-expressed 1-like
14746840.3291536ephrin-A1
18438430.3290269KIAA1304 protein
2663120.3289707ATPase, Cu++ transporting, beta polypeptide (Wilson disease)
1356400.3288537syntaxin 3A
2797200.3282308ESTs, Moderately similar to A47582 B-cell growth factor
precursor [H. sapiens]
7248880.3272422cytochrome P450, subfamily IVB, polypeptide 1
8118080.326728nucleoside diphosphate kinase type 6 (inhibitor of p53-induced
apoptosis-alpha)
1315660.3261265hypothetical protein MGC11061
1544660.3258377STIP1 homology and U-Box containing protein 1
16921950.3254467smg GDS-ASSOCIATED PROTEIN
7853680.3254362PDZ-binding kinase; T-cell originated protein kinase
16375160.325364nuclear autoantigen
16378290.3246232ESTs
8105670.3243984Homo sapiens, clone MGC: 3182 IMAGE: 3356293, mRNA,
complete cds
10272830.3242547ESTs
3068060.324179Homo sapiens, Similar to hypothetical protein FLJ12838, clone
IMAGE: 4130879, mRNA, partial cds
8554060.3241172Homo sapiens clone TCCCIA00176 mRNA sequence
4292220.3239231CGI-107 protein
15005420.3228485regulator of G-protein signalling 11
2696060.3228482N-methylpurine-DNA glycosylase
745660.3226444exportin 1 (CRM1, yeast, homolog)
7541920.3225824Homo sapiens mRNA; cDNA DKFZp586H0924 (from clone
DKFZp586H0924)
1807850.322529KIAA0726 gene product
8244790.3225263exonuclease NEF-sp
8428630.3225116N-myc downstream regulated
17080550.3225042glioblastoma overexpressed
16311940.3219004t-complex-associated-testis-expressed 1-like 1
3668340.3217743envoplakin
8395800.3216628ESTs
2454850.3215041hypothetical protein MGC5178
8155010.3214849hypothetical protein MGC2721
20174030.3211799regulator of G-protein signalling 3
2814760.3210873aspartylglucosaminidase
1308350.3209345Homo sapiens, Similar to clone FLB3816, clone
IMAGE: 3454380, mRNA
775330.3205713inositol polyphosphate-5-phosphatase, 40 kD
420760.3194462TRK-fused gene
8109980.3179036DKFZP434C131 protein
75859−1.8328232N-myc downstream-regulated gene 2
760299−1.7010462dickkopf (Xenopus laevis) homolog 3
344720−1.5196431glycophorin C (Gerbich blood group)
345670−1.4445337ESTs, Moderately similar to I59348 CCAAT binding
transcription factor CBF subunit C - rat [R. norvegicus]
200814−1.4325283membrane metallo-endopeptidase (neutral endopeptidase,
enkephalinase, CALLA, CD10)
1882697−1.4318896peanut (Drosophila)-like 2
1161564−1.3891189desmuslin
788234−1.3761312inhibitor of DNA binding 4, dominant negative helix-loop-helix
protein
1558675−1.3749762SRY (sex determining region Y)-box 10
285377−1.3699153pellino (Drosophila) homolog 2
45099−1.3515907regucalcin (senescence marker protein-30)
611532−1.3158379troponin I, skeletal, fast
377275−1.3102234ataxia-telangiectasia group D-associated protein
810002−1.3038896Homo sapiens, clone MGC: 19762 IMAGE: 3636045, mRNA,
complete cds
1569187−1.2971252heparan sulfate (glucosamine) 3-O-sulfotransferase 4
377461−1.2798035caveolin 1, caveolae protein, 22 kD
712139−1.2738321ADP-ribosylation factor-like 7
742685−1.272916disabled (Drosophila) homolog 2 (mitogen-responsive
phosphoprotein)
811920−1.2690713interleukin 11 receptor, alpha
796542−1.2625621ets variant gene 5 (ets-related molecule)
486683−1.2614406Homo sapiens mRNA; cDNA DKFZp564J0323 (from clone
DKFZp564J0323)
753071−1.2529315Homo sapiens cDNA: FLJ22528 fis, clone HRC12825
131839−1.2372478folate receptor 1 (adult)
300632−1.2368481hypothetical protein FLJ21044 similar to Rbig1
839736−1.2271618crystallin, alpha B
813265−1.2230435Homo sapiens mRNA; cDNA DKFZp564H1916 (from clone
DKFZp564H1916)
1469377−1.2127781lipoma HMGIC fusion partner-like 2
490023−1.2114805hypothetical protein MGC2648
298122−1.1962478frizzled (Drosophila) homolog 7
72778−1.1752838caspase 7, apoptosis-related cysteine protease
160192−1.1751869ESTs, Weakly similar to 2004399A chromosomal protein
[H. sapiens]
22917−1.1590598Homo sapiens mRNA; cDNA DKFZp761M0111 (from clone
DKFZp761M0111)
877621−1.1501074nGAP-like protein
840266−1.1476896Homo sapiens cDNA: FLJ22667 fis, clone HSI08385
289760−1.1390686ESTs, Highly similar to T00391 hypothetical protein KIAA0612
[H. sapiens]
811848−1.1350073hypothetical protein
1556433−1.1222978GRO3 oncogene
838478−1.1175047neurocalcin delta
1473274−1.1172693myosin regulatory light chain 2, smooth muscle isoform
1917449−1.0894686serum amyloid A4, constitutive
811088−1.0851546ephrin-B3
1554167−1.0818834hypothetical protein FLJ14529
67741−1.0705249PP2135 protein
2106144−1.067765regulated in glioma
2056139−1.0673271LIM domain protein
767202−1.0654145latent transforming growth factor beta binding protein 2
345034−1.0600859small inducible cytokine subfamily B (Cys-X-Cys), member 14
(BRAK)
666879−1.0463245annexin A8
130201−1.0401997intercellular adhesion molecule 2
878836−1.037352secretory granule, neuroendocrine protein 1 (7B2 protein)
52419−1.0368509Friedreich ataxia region gene X123
291478−1.0212528runt-related transcription factor 3
781014−1.0161379suppression of tumorigenicity 5
132857−1.0084069Homo sapiens mRNA; cDNA DKFZp586N1323 (from clone
DKFZp586N1323)
529843−1.0036824ESTs, Moderately similar to JC5238 galactosylceramide-like
protein, GCP [H. sapiens]
2504881−1.000863signal transducer and activator of transcription 5A
69002−0.9960916PPAR(gamma) angiopoietin related protein
841308−0.9953716myosin, light polypeptide kinase
488404−0.9880176Homo sapiens clone TUA8 Cri-du-chat region mRNA
277571−0.9841522KIAA1706 protein
1871116−0.9821709Homo sapiens mRNA; cDNA DKFZp434C1714 (from clone
DKFZp434C1714); partial cds
897963−0.9775616phosphatidic acid phosphatase type 2A
1584540−0.9754693Homo sapiens mRNA; cDNA DKFZp586M2022 (from clone
DKFZp586M2022)
196435−0.9665947ESTs
140574−0.9652218small inducible cytokine subfamily D (Cys-X3-Cys), member 1
(fractalkine, neurotactin)
740620−0.96496tropomyosin 2 (beta)
1473471−0.9623482KIAA0194 protein
1635320−0.9586226amiloride-sensitive cation channel 2, neuronal
416959−0.957248nuclear factor I/B
814798−0.9559895aldehyde dehydrogenase 1 family, member A3
823871−0.9429443SPARC-like 1 (mast9, hevin)
303109−0.9414602purinergic receptor (family A group 5)
270826−0.9389651Homo sapiens cDNA FLJ13329 fis, clone OVARC1001795
360254−0.9286811cysteine-rich, angiogenic inducer, 61
66491−0.9280245plasmolipin
814316−0.9276916ribosomal protein L13
153760−0.9227865EphB1
811149−0.9216006chromosome 9 open reading frame 3
786069−0.9203158beta-site APP-cleaving enzyme
208718−0.9190244annexin A1
416676−0.9140494pellino (Drosophila) homolog 1
248631−0.9106505aminomethyltransferase (glycine cleavage system protein T)
76182−0.9106484hypothetical protein DKFZp761F241
756708−0.910505potassium intermediate/small conductance calcium-activated
channel, subfamily N, member 4
712401−0.9068788phosphoinositide-3-kinase, catalytic, delta polypeptide
625399−0.9066615hypothetical protein similar to beta-transducin family
753038−0.893161kinesin family member C3
162308−0.8910356ESTs
796181−0.8888496growth arrest-specific 6
133236−0.8888316RNA binding motif protein, X chromosome
415816−0.8887425ESTs
323780−0.8879854Homo sapiens cDNA FLJ11177 fis, clone PLACE1007402
1493218−0.884795hypothetical protein FLJ22297
1636166−0.8845211KIAA0668 protein
1856063−0.8833091tweety (Drosophila) homolog 1
34093−0.8800259EST
2028722−0.8800075osteoblast specific factor 2 (fasciclin I-like)
811837−0.8764231eukaryotic translation elongation factor 1 alpha 1
628955−0.8761473forkhead box O1A (rhabdomyosarcoma)
884462−0.8743987Down syndrome critical region gene 1
306798−0.8718483NGFI-A binding protein 1 (EGR1 binding protein 1)
882248−0.8700731transgelin
770935−0.8700104hypothetical protein FLJ13511
41208−0.865227bone morphogenetic protein 1
768571−0.8572018SRY (sex determining region Y)-box 8
781017−0.8526926early growth response 2 (Krox-20 (Drosophila) homolog)
811837−0.848137
815737−0.8444322ATP synthase, H+ transporting, mitochondrial F1 complex,
alpha subunit, isoform 1, cardiac muscle
814826−0.8443265ESTs
767164−0.842198ESTs, Weakly similar to MUC2_HUMAN MUCIN 2
PRECURSOR [H. sapiens]
71087−0.8416951v-maf musculoaponeurotic fibrosarcoma (avian) oncogene
family, protein F
283023−0.8413949chemokine (C—X3-C) receptor 1
160609−0.8405669ESTs
814443−0.8385965hypothetical protein MGC3232
142259−0.8376566tumor necrosis factor alpha-inducible cellular protein containing
leucine zipper domains; Huntingtin interacting protein L;
transcrption factor IIIA-interacting protein
990881−0.8335353
970590−0.8333968Homo sapiens mRNA; cDNA DKFZp434A115 (from clone
DKFZp434A115)
470393−0.830751matrix metalloproteinase 7 (matrilysin, uterine)
772913−0.8306579calreticulin
741139−0.8299902eyes absent (Drosophila) homolog 2
27769−0.8298821ESTs
544639−0.8275232ESTs
1587710−0.8252065period (Drosophila) homolog 1
757191−0.8247438ESTs
80344−0.8243418interleukin 7 receptor
1635062−0.8243024DKFZP586A011 protein
119290−0.8224756cortic al thymocyte receptor (X. laevis CTX) like
161456−0.8215502serum amyloid A1
1909935−0.8169271ESTs
1257131−0.8163443ESTs
868396−0.808677tyrosine 3-monooxygenase/tryptophan 5-monooxygenase
activation protein, eta polypeptide
1609665−0.8086161BarH-like homeobox 2
2016775−0.8063264G protein-coupled receptor, family C, group 5, member B
796475−0.8041136four and a half LIM domains 3
1555924−0.8025163CSR1 protein
416434−0.8006527SCN Circadian Oscillatory Protein (SCOP)
322561−0.7970805ribosomal protein L31
132711−0.7962294Kruppel-like factor 5 (intestinal)
70245−0.795375Homo sapiens mRNA full length insert cDNA clone
EUROIMAGE 50374
283124−0.7933772ESTs, Moderately similar to LONG-CHAIN FATTY ACID
TRANSPORT PROTEIN [M. musculus]
281190−0.7927001ESTs
250883−0.7925068ubiquitin-activating enzyme E1-like
346545−0.7887371laminin, beta 1
1878409−0.7869766catechol-O-methyltransferase
530958−0.7809384smoothened (Drosophila) homolog
40027−0.7779473ESTs
788136−0.7778375phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-
homolog phosphodiesterase E4)
1626304−0.7769866spinal cord-derived growth factor-B
1474337−0.7766266phosphorylase, glycogen; brain
85840−0.7754435nicotinamide N-methyltransferase
290378−0.7751825podocalyxin-like
2243051−0.7736287kallikrein 8 (neuropsin/ovasin)
813256−0.7731864ATP-binding cassette, sub-family B (MDR/TAP), member 1
47043−0.7729643tensin
343695−0.7706809hypothetical protein FLJ10875
491403−0.7700599tumor necrosis factor receptor superfamily, member 1B
767765−0.7691523GTP-binding protein overexpressed in skeletal muscle
681992−0.7691299Homo sapiens cDNA FLJ13384 fis, clone PLACE1001062,
highly similar to Homo sapiens mRNA for lysine-ketoglutarate
reductase/saccharopine dehydrogenase
505864−0.7677235RalGDS-like gene
139660−0.7677153ESTs
869450−0.7659922ribosomal protein L11
162308−0.7654139
209537−0.763749zinc finger protein 221
753162−0.7617972KIAA0603 gene product
810728−0.7607171hypothetical gene ZD52F10
366591−0.7595643T-cell lymphoma invasion and metastasis 2
488258−0.7574184collagen, type XVI, alpha 1
32489−0.7571259hypothetical protein DKFZp566A1524
1519147−0.7548183
418159−0.7545888synaptogyrin 1
80727−0.7538606receptor tyrosine kinase-like orphan receptor 1
593023−0.7516846dystrobrevin, beta
586803−0.7423407placental growth factor, vascular endothelial growth factor-
related protein
188335−0.7399294egf-like module containing, mucin-like, hormone receptor-like
sequence 2
1161775−0.7380353villin 1
490668−0.7364434Homo sapiens mRNA; cDNA DKFZp586B211 (from clone
DKFZp586B211)
1474900−0.736252keratin 15
378813−0.7346408secretory leukocyte protease inhibitor (antileukoproteinase)
1630990−0.7312981ribosomal protein L29
280907−0.7307519Kruppel-type zinc finger protein
742562−0.730047chromosome 16 open reading frame 5
73252−0.72838983-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
26566−0.7278186protein-O-mannosyltransferase 1
1471829−0.7275378uncharacterized hypothalamus protein HSMNP1
289428−0.7270494neurotrophic tyrosine kinase, receptor, type 2
898092−0.7267434connective tissue growth factor
34150−0.7263513ESTs
24958−0.7225195Homo sapiens mRNA; cDNA DKFZp434C2016 (from clone
DKFZp434C2016)
725680−0.7197488transcription factor AP-2 gamma (activating enhancer-binding
protein 2 gamma)
77972−0.7194713complement component 3
1686766−0.7192745Rag D protein
471196−0.7192268integral membrane protein 3
431231−0.7182897EGF-containing fibulin-like extracellular matrix protein 2
1908834−0.7177051ubiquitin A-52 residue ribosomal protein fusion product 1
207735−0.7167416serine (or cysteine) proteinase inhibitor, clade A (alpha-1
antiproteinase, antitrypsin), member 1
754157−0.7165332Homo sapiens mRNA; cDNA DKFZp434K2172 (from clone
DKFZp434K2172)
35828−0.7163689diphtheria toxin receptor (heparin-binding epidermal growth
factor-like growth factor)
1946448−0.7150027caveolin 2
470148−0.712917ESTs
840944−0.7099353early growth response 1
809784−0.7095414kallikrein 6 (neurosin, zyme)
877827−0.7093855ribosomal protein S27a
32493−0.7093777integrin, alpha 6
813520−0.7092422EphB3
782501−0.7086066hypothetical protein PP1665
27544−0.708383prominin (mouse)-like 1
184022−0.7070266amyloid beta (A4) precursor protein-binding, family B, member
1 (Fe65)
323238−0.7042807GRO1 oncogene (melanoma growth stimulating activity, alpha)
488956−0.70192CUG triplet repeat, RNA-binding protein 2
1926246−0.701769ESTs, Moderately similar to T46371 hypothetical protein
DKFZp434P1018.1 [H. sapiens]
325365−0.7012106HIV-1 rev binding protein 2
221846−0.6982802checkpoint suppressor 1
1870305−0.6958213heat shock 27 kD protein 2
277044−0.6947855KIAA1183 protein
503335−0.6944661hypothetical protein FLJ11196
757873−0.6940266cyclin-dependent kinase 5, regulatory subunit 1 (p35)
2306682−0.6934667A kinase (PRKA) anchor protein (yotiao) 9
1864302−0.6932327E74-like factor 5 (ets domain transcription factor)
843070−0.6925728nucleoporin 88 kD
812975−0.6921755KIAA0172 protein
46129−0.6919169ESTs, Highly similar to T17245 hypothetical protein
DKFZp586J0917.1 [H. sapiens]
270917−0.6918383secreted frizzled-related protein 1
789382−0.6898974Notch (Drosophila) homolog 4
611481−0.6894079HMG-box transcription factor TCF-3
35300−0.688788KIAA0869 protein
50892−0.6887497Homo sapiens, clone MGC: 9913 IMAGE: 3870821, mRNA,
complete cds
366100−0.6885372matrilin 2
345056−0.6863529KIAA1404 protein
2783721−0.6854649cholinergic receptor, nicotinic, beta polypeptide 1 (muscle)
178137−0.6839022ribosomal protein L34
1626996−0.682449c-fos induced growth factor (vascular endothelial growth factor
D)
188388−0.6824191integrin, alpha 10
133518−0.6817662microtubule-associated protein, RP/EB family, member 2
839796−0.6807912candidate tumor suppressor p33 ING1 homolog
726582−0.6807781actin-related protein 3-beta
50586−0.6794282KIAA1545 protein
491763−0.679034interleukin 1, beta
52096−0.6789228platelet-derived growth factor receptor, alpha polypeptide
263716−0.6767498collagen, type VI, alpha 1
2095066−0.676021alcohol dehydrogenase 1C (class I), gamma polypeptide
1475738−0.6753091ribosomal protein S25
41869−0.6747514hypothetical protein FLJ11017
1350439−0.6740833KIAA0015 gene product
502689−0.6739476Homo sapiens clone HH409 unknown mRNA
773373−0.6730112hypothetical protein MGC14258
308539−0.6726187Homo sapiens cDNA FLJ12777 fis, clone NT2RP2001720
343760−0.6724144SH3 domain binding glutamic acid-rich protein like 2
769959−0.6720196collagen, type IV, alpha 2
2252954−0.6687686enhancer of filamentation 1 (cas-like docking; Crk-associated
substrate related)
70201−0.6675012mitochondrial solute carrier
505243−0.6671218inositol 1,4,5-triphosphate receptor, type 2
897731−0.6670507latrophilin
296880−0.6655878membrane protein, palmitoylated 1 (55 kD)
1486082−0.6635259heparin-binding growth factor binding protein
1470278−0.6633411hypothetical protein FLJ21841
177665−0.6624986
725152−0.6591675hypothetical protein DKFZp762A227
247616−0.6585351lipoma HMGIC fusion partner
752668−0.65723KIAA0440 protein
837870−0.657186proline arginine-rich end leucine-rich repeat protein
744918−0.6552361Homo sapiens mRNA; cDNA DKFZp761I0911 (from clone
DKFZp761I0911)
39600−0.6540275adenylate kinase 5
795288−0.6528792ubiquitin specific protease 4 (proto-oncogene)
1665444−0.651994tumor endothelial marker 1 precursor
774078−0.6508021leiomodin 1 (smooth muscle)
415233−0.6506593ribosomal protein L37a
307029−0.6500872ribosomal protein L26
78946−0.648366Homo sapiens pyruvate dehydrogenase kinase 4 mRNA, 3′
untranslated region, partial sequence
785733−0.6476493hypothetical protein FLJ12892
415415−0.646492EST, Moderately similar to RL1X_HUMAN 60S RIBOSOMAL
PROTEIN L18A [H. sapiens]
858167−0.6453859fatty-acid-Coenzyme A ligase, long-chain 4
823714−0.642903nuclear receptor co-repressor/HDAC3 complex subunit
193913−0.6425581v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
220395−0.6418175hypothetical protein FLJ23293 similar to ARL-6 interacting
protein-2
840942−0.6383764major histocompatibility complex, class II, DP beta 1
725308−0.6371261ras inhibitor
1492147−0.636656ribosomal protein S4, X-linked
1466893−0.6364598ESTs
75078−0.6362307ESTs
796152−0.6356106Homo sapiens cDNA FLJ11685 fis, clone HEMBA1004934
190059−0.634814guanine nucleotide binding protein (G protein), gamma 7
840511−0.6341661vimentin
81409−0.6338327GABA(A) receptor-associated protein like 1
745490−0.6329354hypothetical protein FLJ20607
530036−0.6324794hypothetical protein FLJ13078
755855−0.6298333solute carrier family 25 (mitochondrial carrier; adenine
nucleotide translocator), member 6
785538−0.6289951Homo sapiens mRNA; cDNA DKFZp434N2116 (from clone
DKFZp434N2116)
2284803−0.6265431secretory carrier membrane protein 1
1623016−0.6263561EST
154472−0.6253276fibroblast growth factor receptor 1 (fms-related tyrosine kinase
2, Pfeiffer syndrome)
774754−0.6243946catenin (cadherin-associated protein), beta 1 (88 kD)
1572298−0.6228686CD3Z antigen, zeta polypeptide (TiT3 complex)
2252417−0.6222381ribosomal protein S10
180803−0.621787inositol polyphosphate-1-phosphatase
683059−0.6216483Cdc42 effector protein 3
1577736−0.6215965epidermal growth factor (beta-urogastrone)
241489−0.6212938adrenergic, beta-2-, receptor, surface
2316441−0.6206597ras homolog gene family, member C
1712825−0.6204409ESTs
52724−0.6199204hypothetical protein FLJ20241
150897−0.6188264UDP-GIcNAc: betaGal beta-1,3-N-
acetylglucosaminyltransferase 3
823851−0.6182341AE-binding protein 1
810057−0.6178096vasoactive intestinal peptide receptor 1
81316−0.6176265ESTs
298134−0.6152731frizzled (Drosophila) homolog 1
2028238−0.6141182KIAA0300 protein
1898758−0.6126872BCE-1 protein
250797−0.6124667hypothetical protein FLJ20038
754040−0.610807NS1-associated protein 1
1584628−0.6102937alpha-actinin-2-associated LIM protein
230910−0.6096107
172783−0.6088873hypothetical protein FLJ10390
824799−0.6054867ART-4 protein
785967−0.6049171erythrocyte membrane protein band 4.1-like 2
342349−0.603487mitogen-activated protein kinase kinase kinase 14
67067−0.6003961Homo sapiens mRNA; cDNA DKFZp564O222 (from clone
DKFZp564O222)
1533710−0.5988282ortholog of mouse integral membrane glycoprotein LIG-1
768007−0.5983563hypothetical protein
1707637−0.5964339ESTs
1634832−0.5931096class I cytokine receptor
1609625−0.5920312selectin P ligand
1592530−0.5918818mammalian inositol hexakisphosphate kinase 2
272706−0.5914377alpha2,3-sialyltransferase
897720−0.5904719trophinin
1472735−0.5888169metallothionein 1E (functional)
767641−0.5881449mitogen-activated protein kinase 8 interacting protein 2
1651906−0.5863631hypothetical protein FLJ23544
564801−0.5824549Homo sapiens cDNA: FLJ21409 fis, clone COL03924
248886−0.5823315rab3 GTPase-activating protein, non-catalytic subunit (150 kD)
250654−0.5815576secreted protein, acidic, cysteine-rich (osteonectin)
1968422−0.5802058Homo sapiens mRNA full length insert cDNA clone
EUROIMAGE 1968422
712829−0.5797871LIM domain only 2 (rhombotin-like 1)
752837−0.5794612Homo sapiens mRNA for FLJ00074 protein, partial cds
346902−0.5794362ESTs, Weakly similar to ALU2_HUMAN ALU SUBFAMILY SB
SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
782193−0.5788147thioredoxin
358531−0.5784457v-jun avian sarcoma virus 17 oncogene homolog
2271240−0.5782432hypothetical protein
66532−0.5773248endothelin 3
120106−0.5770131caspase 1, apoptosis-related cysteine protease (interleukin 1,
beta, convertase)
810552−0.5768989B-cell associated protein
770848−0.5767154ESTs, Weakly similar to ALU1_HUMAN ALU SUBFAMILY J
SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
1032831−0.5764161gycosyltransferase
460403−0.5739604laminin, gamma 2 (nicein (100 kD), kalinin (105 kD), BM600
(100 kD), Herlitz junctional epidermolysis bullosa))
489755−0.5730453a disintegrin and metalloproteinase domain 12 (meltrin alpha)
502753−0.5724623angiopoietin 2
68534−0.5724394Homo sapiens cDNA: FLJ22290 fis, clone HRC04405
854645−0.5723062CDC-like kinase 3
322024−0.5714933ESTs, Highly similar to T12495 hypothetical protein
DKFZp434H071.1 [H. sapiens]
140071−0.5708303frizzled-related protein
788518−0.5697316peroxisomal membrane protein 3 (35 kD, Zellweger syndrome)
240752−0.56913hypothetical protein MGC14797
773487−0.5686324hypothetical protein MGC3032
1404774−0.5685596parathyroid hormone-like hormone
814815−0.5675649plakophilin 4
462939−0.5662456ESTs
240248−0.5660209transferrin
208001−0.5640857CD59 antigen p18-20 (antigen identified by monoclonal
antibodies 16.3A5, EJ16, EJ30, EL32 and G344)
1909433−0.5638242DKFZP727C091 protein
1636360−0.5629882hypothetical protein FLJ14957
138242−0.5621088ESTs, Moderately similar to MAS2_HUMAN MANNAN-
BINDING LECTIN SERINE PROTEASE 2 PRECURSOR
[H. sapiens]
703964−0.5620699inositol polyphosphate phosphatase-like 1
1522734−0.5618289ESTs
843094−0.5617478ubiquitin-like 1 (sentrin)
491519−0.561401Homo sapiens clone 24775 mRNA sequence
377314−0.561312casein kinase 2, alpha prime polypeptide
208969−0.561267EST
788558−0.5600825KIAA1479 protein
785530−0.5600048integrin, alpha 1
1473131−0.5594101transducin-like enhancer of split 2, homolog of Drosophila
E(sp1)
812959−0.5591846KIAA1638 protein
868169−0.5584677lipoprotein lipase
322233−0.5578912ribosomal protein, large, PO
50519−0.5577761aryl hydrocarbon receptor nuclear translocator-like
299720−0.5565137
215000−0.555725vasoactive intestinal peptide receptor 1
51981−0.5554908ribosomal protein L7a
703541−0.5534921KIAA1858 protein
238907−0.5534598hypothetical protein, clone
Telethon(Italy_B41)_Strait02270_FL142
415613−0.5528761DHHC1 protein
282404−0.5528532Homo sapiens mRNA for KIAA1671 protein, partial cds
344588−0.55146kallikrein 5
746373−0.5513143RNA polymerase I transcription factor RRN3
491186−0.5507413Homo sapiens cDNA: FLJ23131 fis, clone LNG08502
75059−0.5504962poly(A)-binding protein, cytoplasmic 1-like
345764−0.5504113special AT-rich sequence binding protein 1 (binds to nuclear
matrix/scaffold-associating DNA's)
1577920−0.5496944ESTs
288748−0.5493871Homo sapiens, clone MGC: 5352 IMAGE: 3048106, mRNA,
complete cds
810097−0.5487174Homo sapiens cDNA: FLJ21721 fis, clone COLF0381
755881−0.5473964aquaporin 5
1568391−0.5467858plastin 3 (T isoform)
290866−0.5467613v-raf-1 murine leukemia viral oncogene homolog 1
503671−0.5461871Homo sapiens cDNA FLJ14368 fis, clone HEMBA1001122
2388571−0.5459823A kinase (PRKA) anchor protein 8
739193−0.5457998cellular retinoic acid-binding protein 1
756372−0.5447652retinoic acid receptor responder (tazarotene induced) 2
53081−0.5446617ESTs
486179−0.5443525Homo sapiens cDNA FLJ10205 fis, clone HEMBA1004954
84464−0.5426397hypothetical protein FLJ12806
2213824−0.5412089protease inhibitor 3, skin-derived (SKALP)
1597813−0.5406089hypothetical protein PP1044
810358−0.5403705acyl-Coenzyme A dehydrogenase, very long chain
341759−0.5403406lung type-I cell membrane-associated glycoprotein
309449−0.5401954ribosomal protein S4, Y-linked
42681−0.5388424NY-REN-25 antigen
299720−0.5388238eukaryotic translation elongation factor 1 alpha 1
162775−0.5385174DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA
helicase, 68 kD)
490102−0.5383385TBP-associated factor 172
877835−0.5382125ribosomal protein L35
299720−0.5378801eukaryotic translation elongation factor 1 alpha 1
1534493−0.5376323ESTs
595637−0.5373697Homo sapiens mRNA; cDNA DKFZp586N012 (from clone
DKFZp586N012)
725622−0.5370309ESTs
233679−0.5356625hypothetical protein FLJ22362
785933−0.5340762sushi-repeat-containing protein, X chromosome
825356−0.533771Homo sapiens cDNA FLJ11997 fis, clone HEMBB1001458
767982−0.533231Homo sapiens, clone IMAGE: 4134852, mRNA, partial cds
277627−0.5329107Human SH3 domain-containing protein SH3P18 mRNA,
complete cds
460126−0.5320146K1AA0819 protein
346688−0.5315035melanoma inhibitory activity
712023−0.5302372AT-binding transcription factor 1
24729−0.5302207cholinergic receptor, muscarinic 1
1590021−0.5302114ets variant gene 6 (TEL oncogene)
809998−0.5297152
811779−0.5291718phosphoinositide-3-kinase, regulatory subunit, polypeptide 1
(p85 alpha)
488130−0.5289721Homo sapiens cDNA FLJ20767 fis, clone COL06986
299720−0.5284407
159462−0.528299serum constituent protein
1570427−0.5280955hypothetical protein MGC4309
2308346−0.5268833cyclin-dependent kinase 2
1881774−0.5265891KIAA1678
591055−0.5258289protein kinase C and casein kinase substrate in neurons 2
470128−0.5253542myosin IE
687990−0.5253354Rac/Cdc42 guanine exchange factor (GEF) 6
753620−0.5248489insulin-like growth factor binding protein 6
80374−0.5236475pyruvate dehydrogenase (lipoamide) alpha 1
878421−0.5233873transgelin 2
343079−0.5224833Homo sapiens mRNA; cDNA DKFZp761P0114 (from clone
DKFZp761P0114)
284592−0.5205573PRO1659 protein
855061−0.5203046vascular endothelial growth factor B
183704−0.5184644Homo sapiens, clone MGC: 13446 IMAGE: 4275731, mRNA,
complete cds
155896−0.5182247ORF
842939−0.5172329adenosine deaminase, RNA-specific, B1 (homolog of rat RED1)
685185−0.5172158reticulon 2
753301−0.5171758carcinoembryonic antigen-related cell adhesion molecule 1
(biliary glycoprotein)
795178−0.5169102lactate dehydrogenase C
1911663−0.5168962ESTs
345023−0.5166203ESTs, Highly similar to T08701 hypothetical protein
DKFZp564N123.1 [H. sapiens]
1631682−0.5162406peptidylprolyl isomerase E (cyclophilin E)
868308−0.5157133ribosomal protein S23
809998−0.5144681amylase, alpha 2A; pancreatic
771004−0.5144276K1AA1201 protein
842896−0.5137089hypothetical protein DKFZp762L0311
714213−0.5129593tumor necrosis factor receptor superfamily, member 6
197056−0.5128128ESTs
415191−0.512457KIAA0161 gene product
2306752−0.5123647stathmin-like 2
469306−0.5113377gastrin-releasing peptide
824602−0.5107682interferon, gamma-inducible protein 16
307687−0.5106552protease, serine, 16 (thymus)
1637296−0.5105214ribosomal protein S24
1358393−0.5090372mitogen-activated protein kinase kinase 3
504940−0.508056
713839−0.5080365transcription factor AP-4 (activating enhancer-binding protein 4)
220293−0.5078291Homo sapiens cDNA: FLJ21800 fis, clone HEP00618
125342−0.5078237SUMO-1-specific protease
1601845−0.5074894Ca2+-promoted Ras inactivator
240620−0.5065007vascular Rab-GAP/TBC-containing
43679−0.5063146ESTs
825013−0.505654acidic protein rich in leucines
2116188−0.5046972histone deacetylase 5
161998−0.5044691hypothetical protein FLJ23138
83999−0.5044612Human DNA sequence from clone 889N15 on chromosome
Xq22.1-22.3. Contains part of the gene for a novel protein
similar to X. laevis Cortical Thymocyte Marker CTX, the
possibly alternatively spliced gene for 26S Proteasome subunit
p28 (Ankyrin repeat prote
593840−0.5033966DKFZP564K1964 protein
51448−0.5031992activating transcription factor 3
383501−0.502319regulator of G-protein signalling 9
2018423−0.501705death-associated protein kinase 2
796984−0.5010588cytochrome b-245, beta polypeptide (chronic granulomatous
disease)
1160723−0.5005952LIM domain kinase 2
414999−0.5002542ets variant gene 4 (E1A enhancer-binding protein, E1AF)
770212−0.4981295chitinase 3-like 1 (cartilage glycoprotein-39)
48518−0.4966991ATP-binding cassette, sub-family A (ABC1), member 5
85634−0.4961026complement component 1, s subcomponent
341763−0.4953701caspase 5, apoptosis-related cysteine protease
812161−0.4948424ESTs
1417886−0.4941304hypothetical protein FLJ23239
1500815−0.4934665Homo sapiens cDNA: FLJ22130 fis, clone HEP19632
868400−0.4928479glutaminyl-tRNA synthetase
809894−0.491862acetyl-CoA synthetase
753745−0.4917192ESTs, Weakly similar to S57447 HPBRII-7 protein [H. sapiens]
85394−0.4913773phosphatidic acid phosphatase type 2B
134192−0.4908497ESTs
813698−0.4898644sprouty (Drosophila) homolog 2
253884−0.4897811Human BAC clone GS1-99H8
131012−0.4897744hypothetical protein FLJ10633
855755−0.4897236fibrillarin
1711456−0.4896574H factor (complement)-like 1
815142−0.4881454spastic ataxia of Charlevoix-Saguenay (sacsin)
2413337−0.4879268sortilin-related receptor, L(DLR class) A repeats-containing
327432−0.4870045semaphorin Y
1897944−0.4855156ESTs, Weakly similar to T00366 hypothetical protein KIAA0669
[H. sapiens]
|
Example VI
[0108] Genes for Discriminating Between Normal and ADH (Non-Malignant) Versus DCIS and IDC (Malignant)
[0109] As shown in Table 5 below, 400 genes were identified as being able to discriminate normal and ADH (non-malignant) versus DCIS and IDC (malignant).
5TABLE 5
|
|
CloneIDWeightDescription
|
|
7964691.4207633HSPC150 protein similar to ubiquitin-
conjugating enzyme
4889641.3447179H2A histone family, member O
15050381.3277637hypothetical protein FLJ20171
15000001.2926116H2B histone family, member B
15545491.2787033hydroxyacyl glutathione hydrolase
8122381.2664748hypothetical protein MGC4692
351471.2581066ESTs, Weakly similar to unnamed protein
product [H. sapiens]
1220771.2576139putative membrane protein
7886541.2573483growth factor receptor-bound protein 2
5950371.2486446retinoic acid induced 3
5653191.2155833Homo sapiens mRNA; cDNA DKFZp564B1264
(from clone DKFZp564B1264)
2839191.2112507H2A histone family, member L
19179411.1872008purine-rich element binding protein B
3598871.1837896translocase of inner mitochondrial membrane
17 (yeast) homolog A
4715681.1673113hematological and neurological expressed 1
2908411.1670252H2B histone family, member A
7966941.1580364baculoviral IAP repeat-containing 5
(survivin)
3661321.1440486succinate dehydrogenase complex, subunit C,
integral membrane protein, 15 kD
8235981.1425746proteasome (prosome, macropain) 26S subunit,
non-ATPase, 12
13234481.1309009cysteine-rich protein 1 (intestinal)
8107111.1074523stearoyl-CoA desaturase (delta-9-desaturase)
7414741.0870449glucose phosphate isomerase
7456061.0663136hypothetical protein PP591
20546351.0613961proteasome (prosome, macropain) subunit,
alpha type, 7
1788051.0513873Human DNA sequence from clone RP5-850E9 on
chromosome 20. Contains part of the gene
for a novel C2H2 type zinc finger protein
similar to Drosophila Scratch (Scrt), Slug
and Xenopus Snail, a novel gene similar to
Drosophila CG6762, STSs, GSSs and five CpG
3473731.0454939transcription elongation factor B (SIII),
polypeptide 1 (15 kD, elongin C)
1994031.0142329lectin, galactoside-binding, soluble, 8
(galectin 8)
8140541.0130821KIAA0040 gene product
20291731.0094499ESTs, Weakly similar to N-WASP
[H. sapiens]
2090661.0066096
7824281.0008279KIAA0250 gene product
7699210.9944462ubiquitin carrier protein E2-C
4700610.9920108seven in absentia (Drosophila) homolog 2
7967230.9915885Homo sapiens clone CDABP0014 mRNA
sequence
16098360.9886168glutamate-ammonia ligase (glutamine
synthase)
23223670.9859632reticulon 4
2803750.9793036PRO2000 protein
20169080.969649ESTs, Weakly similar to CA13_HUMAN
COLLAGEN ALPHA 1(III) CHAIN PRECURSOR
[H. sapiens]
18588920.9669022hypothetical protein MGC4825
462480.9628117ADP-ribosyltransferase (NAD+; poly
(ADP-ribose) polymerase)
20140340.9619198methylene tetrahydrofolate dehydrogenase
(NAD+ dependent), methenyltetrahydrofolate
cyclohydrolase
8980320.9547022KIAA0097 gene product
7254540.9476507CDC28 protein kinase 2
795200.9457391RAB2, member RAS oncogene family
8105580.943041proteasome (prosome, macropain) 26S subunit,
ATPase, 4
2725290.9423688phosphomannomutase 2
1222410.939204proteasome (prosome, macropain) subunit,
beta type, 2
4696860.9381847Ric (Drosophila)-like, expressed in many
tissues
6246670.9336899CGI-92 protein
4882020.932881ESTs, Weakly similar to YZ28_HUMAN
HYPOTHETICAL PROTEIN ZAP128 [H. sapiens]
8254700.9274271topoisomerase (DNA) II alpha (170 kD)
16408210.920874ESTs, Weakly similar to I78885 serine/
threonine-specific protein kinase
[H. sapiens]
6865520.9182272golgi phosphoprotein 1
20166480.917373Homo sapiens mRNA; cDNA DKFZp434N1728
(from clone DKFZp434N1728)
19113430.9110591RAB26, member RAS oncogene family
7810970.9100493reticulon 3
2448010.908481Rho guanine exchange factor (GEF) 11
7546280.9061145ESTs
15740580.90582111-acylglycerol-3-phosphate O-acyltransferase
2 (lysophosphatidic acid acyltransferase,
beta)
7532990.9019921hypothetical protein FLJ10504
8117740.9014801CGI-49 protein
5952130.8978322hypothetical protein
8681280.8970146JM4 protein
8101240.8891272platelet-activating factor acetylhydrolase,
isoform lb, gamma subunit (29 kD)
664060.8888639hypothetical protein DKFZp762E1312
16360920.8841788hypothetical protein FLJ20657
18692010.8825788hypothetical protein MGC2745
6259230.8821749phosphoenolpyruvate carboxykinase 2
(mitochondrial)
14922380.8804306HSPC003 protein
7310440.8716644glutaredoxin 2
8396820.8696528ubiquitin-conjugating enzyme E2N (homologous
to yeast UBC13)
9512330.8614727proteasome (prosome, macropain) subunit,
beta type, 3
8431950.8606568phosphoserine phosphatase
2889990.8593924small protein effector 1 of Cdc42
517730.8588635hypothetical protein MGC3077
2090660.8582298serine/threonine kinase 15
14749550.8573467TATA box binding protein (TBP)-associated
factor, RNA polymerase II, N, 68 kD (RNA-
binding protein 56)
20431670.8551193BCL2-associated athanogene 3
7427070.8515067ESTs, Weakly similar to MUC2_HUMAN MUCIN
2 PRECURSOR [H. sapiens]
7435890.8514377ESTs, Weakly similar to T2D3_HUMAN
TRANSCRIPTION INITIATION FACTOR TFIID
135 KDA SUBUNIT [H. sapiens]
7044140.8498631small nuclear ribonucleoprotein polypeptides
B and B1
23090730.8484971frizzled (Drosophila) homolog 5
20521130.8477245hypothetical protein FLJ10903
6861720.846207KIAA0008 gene product
1500030.8447372hypothetical protein FLJ13187
7050640.8401441transforming, acidic coiled-coil containing
protein 3
17097910.8397779BAI1-associated protein 1
14694250.8391993SRY (sex determining region Y)-box 22
4297990.8386406hypothetical protein FLJ21939 similar to 5-
azacytidine induced gene 2
7299750.8385313meningioma expressed antigen 5
(hyaluronidase)
13930180.8376676general transcription factor IIIC,
polypeptide 1 (alpha subunit, 220 kD)
14924630.8360771selenoprotein X, 1
20289490.8358024hypothetical protein PRO1855
7890120.8351735fibulin 2
4701240.8347241RAD1 (S. pombe) homolog
14095090.8339967troponin T1, skeletal, slow
16054260.8317254hypothetical protein FLJ13352
442920.831599Homo sapiens mRNA; cDNA DKFZp434C107
(from clone DKFZp434C107)
14350030.8311727tumor suppressing subtransferable candidate
1
5032150.8286483pilin-like transcription factor
5043080.8199799hypothetical protein FLJ10540
7857070.8176557protein regulator of cytokinesis 1
15001620.8155519ESTs
1493550.8134342translocating chain-associating membrane
protein
18451690.8131362RAB35, member RAS oncogene family
8693750.808792isocitrate dehydrogenase 2 (NADP+),
mitochondrial
14924260.8032542chromosome 19 open reading frame 3
7825130.8030042interferon, alpha-inducible protein (clone
IFI-6-16)
8132810.8016742WW domain-containing protein 1
8143780.8015236serine protease inhibitor, Kunitz type, 2
7007920.8005506cyclin-dependent kinase inhibitor 3 (CDK2-
associated dual specificity phosphatase)
3405580.7990715actin related protein 2/3 complex, subunit 5
(16 kD)
8429940.7983201cathepsin Z
1381890.7953361Wolfram syndrome 1 (wolframin)
2899780.7931469ubiquitin-like 4
20192230.7893602mitochondrial ribosomal protein L17
21105110.7863117artemin
8145280.7845635Homo sapiens cDNA: FLJ22139 fis, clone
HEP20959
15732510.7816689peroxisomal long-chain acyl-coA thioesterase
7739220.7799164KIAA0005 gene product
1725170.7779159hippocalcin-like 1
5649810.7745626ESTs
14203700.7726877biliverdin reductase B (flavin reductase
(NADPH))
3084660.771216GTP-binding protein Sara
1996450.769591nicastrin
14223380.7690604ribonucleotide reductase M2 polypeptide
14744240.7689082Homo sapiens cDNA FLJ12758 fis, clone
NT2RP2001328
8137510.7665427sialyltransferase 4C (beta-galactosidase
alpha-2,3-sialytransferase)
1310940.7626373Homo sapiens cDNA: FLJ21587 fis, clone
COL06946
8429800.762412developmentally regulated GTP-binding
protein 1
2125420.7585053Homo sapiens cDNA FLJ12900 fis, clone
NT2RP2004321
1084250.7581954
8117610.7578696Nijmegen breakage syndrome 1 (nibrin)
2413480.757138prenylcysteine lyase
8107250.7561061ATPase, H+ transporting, lysosomal
(vacuolar proton pump) 21 kD
14601100.7559869proteasome (prosome, macropain) subunit,
beta type, 5
1207490.7545697ESTs, Moderately similar to KIAA1215 protein
[H. sapiens]
8271710.7543905ESTs
398840.7526496IMP (inosine monophosphate) dehydrogenase 1
1242980.7507816microsomal glutathione S-transferase 3
7533780.7492212hypothetical protein FLJ22649 similar to
signal peptidase SPC22/23
2108620.7483198acyl-Coenzyme A oxidase 1, palmitoyl
7857660.7476331hypothetical protein
15533060.7471557proteasome (prosome, macropain) 26S subunit,
non-ATPase, 11
4700990.7443747HT002 protein; hypertension-related calcium-
regulated gene
833630.7435058protein-L-isoaspartate (D-aspartate) O-
methyltransferase
4893510.7422879hypothetical protein DKFZp566J2046
8106120.7419597S100 calcium-binding protein A11
(calgizzarin)
8255850.741258tubulin-specific chaperone e
14563480.7401571N-acetylneuraminic acid phosphate synthase;
sialic acid synthase
14739220.7399948actin related protein 2/3 complex, subunit 3
(21 kD)
1503140.739762lysophospholipase I
8978060.7388301hypoxia-inducible factor 1, alpha subunit
(basic helix-loop-helix transcription factor)
8115850.738212huntingtin (Huntington disease)
6855160.7378926putative G protein-coupled receptor
766050.7376829nesca protein
14760530.7367106RAD51 (S. cerevisiae) homolog (E coli RecA
homolog)
2212950.7342275inhibitor of DMA binding 2, dominant negative
helix-loop-helix protein
8248790.7333071hypothetical protein MGC11275
7685700.732628hypothetical protein FLJ11280
730090.731401ESTs, Weakly similar to A43932 mucin 2
precursor, intestinal [H. sapiens]
5644920.7301895mitochondrial carrier homolog 2
20174150.7281714centromere protein A (17 kD)
7886540.7244749
4885050.7242619accessory proteins BAP31/BAP29
8249620.7238234karyopherin alpha 2 (RAG cohort 1, importin
alpha 1)
16162530.7231756breast carcinoma amplified sequence 1
14358620.723081antigen identified by monoclonal antibodies
12E7, F21 and O13
7860670.7228184cell division cycle 25B
20508270.7211304proteasome (prosome, macropain) 26S subunit,
ATPase, 5
2486490.7210707hypothetical protein FLJ13910
515320.7195798ADP-ribosylation factor-like 6 interacting
protein
7270780.7184618Homo sapiens cDNA: FLJ23602 fis, clone
LNG15735
377080.7163849hypothetical protein MGC3101
4302350.7162503H2B histone family, member Q
8977700.715754
2929360.7154295hypothetical protein FLJ10468
3657380.7152855ESTs
8453630.714201non-metastatic cells 1, protein (NM23A)
expressed in
8099440.7139515KIAA0310 gene product
16316990.7115561valosin-containing protein
8136290.7102574YME1 (S. cerevisiae)-like 1
8134100.7093173polymerase (RNA) II (DNA directed)
polypeptide K (7.0 kD)
8262560.7092586transmembrane 7 superfamily member 1
(upregulated in kidney)
1243310.7081481cleavage and polyadenylation specific factor
5, 25 kD subunit
7708450.7068283hexokinase 1
677650.7064266carboxypeptidase M
2072880.7058528insulin induced gene 1
16395310.7033264RAB27A, member RAS oncogene family
7310230.7032815WD repeat domain 5
7564420.7016064P450 (cytochrome) oxidoreductase
3581620.6999211protein predicted by clone 23627
7826080.698569mitochondrial ribosomal protein L9
5098230.6982362carcinoembryonic antigen-related cell
adhesion molecule 6 (non-specific cross
reacting antigen)
3457870.6981453highly expressed in cancer, rich in leucine
heptad repeats
8104020.6978141hypothetical protein
7444170.6975761carnitine acetyltransferase
8143060.6963874tumor protein D52
413560.6961169protein phosphatase 2, regulatory subunit B
(B56), alpha isoform
8134190.6951349hydroxyacyl-Coenzyme A dehydrogenase, type II
6299440.6950339myosin VB
3275060.6943362Homo sapiens mRNA full length insert cDNA
clone EUROIMAGE 327506
7680640.6936336cytochrome P450, subfamily I (aromatic
compound-inducible), polypeptide 1
7709920.693225
4693830.6925975chromosome 8 open reading frame 1
14927800.6923749Homo sapiens cDNA FLJ14459 fis, clone
HEMBB1002409
415690.691145hypothetical protein FLJ12650
5095880.6903363TATA box binding protein (TBP)-associated
factor, RNA polymerase II, J, 20 kD
1448800.6902434hypothetical protein from EUROIMAGE 1759349
7893760.6883473thioredoxin reductase 1
2689460.6881593WD40 protein Ciao1
8101560.6879064deoxythymidylate kinase (thymidylate kinase)
842950.6876167interleukin 1 receptor antagonist
2468000.6867481hypothetical protein FLJ10803
5892320.6865999hypothetical protein FLJ11506
8597610.6846134poliovirus receptor-related 2 (herpesvirus
entry mediator B)
4315050.6840493ESTs, Highly similar to A31026 probable
membrane receptor protein [H. sapiens]
1098630.683291epithelial membrane protein 2
7703550.6829507lanosterol synthase (2,3-oxidosqualene-
lanosterol cyclase)
3440910.6818771ESTs
8137070.6814476regulator of G-protein signalling 16
1247810.6809199squalene epoxidase
5027740.6807524hypothetical protein FLJ20623
8257400.6805157DKFZp434J1813 protein
15360060.6805004ESTs
2030030.6802818non-metastatic cells 4, protein expressed in
7856160.6800658signal sequence receptor, alpha (translocon-
associated protein alpha)
7858400.6800437SEC24 (S. cerevisiae) related gene family,
member D
2566190.6797698hydroxysteroid (17-beta) dehydrogenase 7
21080770.6796871CGI-112 protein
7425950.6784804cyclin-dependent kinase 5
14760650.6783519stathmin 1/oncoprotein 18
686360.6778817hypothetical protein MGC2477
5648470.6770455ESTs
7440470.6748419polo (Drosophia)-like kinase
8978130.6741253polyadenylate binding protein-interacting
protein 1
8239300.6738266actin related protein 2/3 complex, subunit
1A (41 kD)
2360340.673506uncoupling protein 2 (mitochondrial, proton
carrier)
7439770.6729967Homo sapiens mRNA for TL132
2937270.6728542hypothetical protein MGC861
5907590.672734sterol-C4-methyl oxidase-like
8110240.6706275bone marrow stromal cell antigen 2
7857950.6683919hypothetical protein FLJ12910
5303100.6682042KIAA0143 protein
17343090.666164sperm associated antigen 4
18986190.6651268hypothetical protein MGC15737
3582670.6648183EST, Moderately similar to AF119917 63
PRO2831 [H. sapiens]
7534000.6633015CGI-204 protein
2927700.6609782Homo sapiens, clone IMAGE: 3627860, mRNA,
partial cds
8844250.6608337chaperonin containing TCP1, subunit 5
(epsilon)
16967570.6599512hypothetical protein KIAA1165
1212510.6596925hypothetical protein MGC5576
7707850.6587511,2-alpha-mannosidase IC
5907740.657447mitogen-activated protein kinase 13
9698770.6564571synaptosomal-associated protein, 25 kD
4151020.6562436cell division cycle 25C
1202710.6562017hypothetical protein MGC4692
7137820.6552297a disintegrin and metalloproteinase domain 15
(metargidin)
493510.6551453SEX gene
4886420.6541915ESTs, Weakly similar to I38022 hypothetical
protein [H. sapiens]
3436070.6510034AD-015 protein
23069870.6501071secreted and transmembrane 1
2802490.64759Kruppel-like factor 7 (ubiquitous)
7526310.6473517fibroblast growth factor receptor 3
(achondroplasia, thanatophoric dwarfism)
4895940.645574hypothetical protein FLJ11565
7731880.6448143nuclear receptor subfamily 1, group D,
member 2
20155170.6441737hypothetical protein FLJ22237
3437310.6424907
4915240.6424448mitochondrial ribosomal protein L13
8245240.6424419UDP-galactose transporter related
5934310.6422897ESTs, Moderately similar to CEGT_HUMAN
CERAMIDE GLUCOSYLTRANSFERASE
[H. sapiens]
8129940.6420817retinoid X receptor, alpha
16317350.6404771Homo sapiens, clone IMAGE: 3604336, mRNA,
partial cds
2785310.6404612cytochrome c oxidase subunit VIc
23020990.6386686sialidase 3 (membrane sialidase)
4548960.6384516DnaJ (Hsp40) homolog, subfamily A, member 2
14567010.6383709B-cell CLL/lymphoma 9
20558070.638184protein kinase domains containing protein
similar to phosphoprotein C8FW
15184020.6378481KIAA1361 protein
8107620.6371461SNARE protein
1244470.6363079KIAA1184 protein
492730.635859solute carrier family 27 (fatty acid
transporter), member 4
3650600.6350631RAB11A, member RAS oncogene family
15912640.6337293transaldolase 1
416980.6327738progesterone binding protein
8103160.6323393very long-chain acyl-CoA synthetase;
lipidosin
8263630.6322533lysophospholipase II
20115150.6319712DKFZP586B0923 protein
7706750.6315109Homo sapiens cDNA: FLJ21323 fis, clone
COL02374
14614770.6300096Homo sapiens mRNA; cDNA DKFZp586I0324
(from clone DKFZp586I0324)
3668340.629867envoplakin
16019470.6297475cytochrome c oxidase subunit VIIa
polypeptide 2 (liver)
5105750.6295917hypothetical protein FLJ22087
5038510.6291771nuclear receptor co-repressor/HDAC3 complex
subunit
8100630.6286184growth factor, erv1 (S. cerevisiae)-like
(augmenter of liver regeneration)
7546530.6277098cadherin, EGF LAG seven-pass G-type receptor
3, flamingo (Drosophila) homolog
15188900.6251884metallothionein-like 5, testis-specific
(tesmin)
7841050.6244805ESTs
2050490.6231646protein kinase H11; small stress protein-like
protein HSP22
3256060.6225147hypothetical protein MGC14353
760299−1.8441097dickkopf (Xenopus laevis) homolog 3
200814−1.8295958membrane metallo-endopeptidase (neutral
endopeptidase, enkephalinase, CALLA, CD10)
1882697−1.715818peanut (Drosophila)-like 2
344720−1.6567437glycophorin C (Gerbich blood group)
1161564−1.5877154desmuslin
45099−1.566311regucalcin (senescence marker protein-30)
75859−1.5303427N-myc downstream-regulated gene 2
811920−1.5255258interleukin 11 receptor, alpha
1569187−1.4872982heparan sulfate (glucosamine) 3-O-
sulfotransferase 4
796542−1.4697418ets variant gene 5 (ets-related molecule)
767202−1.4573536latent transforming growth factor beta
binding protein 2
285377−1.4496786pellino (Drosophila) homolog 2
300632−1.449587hypothetical protein FLJ21044 similar to
Rbig1
160192−1.4231519ESTs, Weakly similar to 2004399A chromosomal
protein [H. sapiens]
611532−1.3689616troponin I, skeletal, fast
813265−1.3632094Homo sapiens mRNA; cDNA DKFZp564H1916
(from clone DKFZp564H1916)
1469377−1.359583lipoma HMGIC fusion partner-like 2
810002−1.3553375Homo sapiens, clone MGC: 19762
IMAGE: 3636045, mRNA, complete cds
2056139−1.3506352LIM domain protein
377275−1.3392122ataxia-telangiectasia group D-associated
protein
298122−1.3364021frizzled (Drosophila) homolog 7
72778−1.3299927caspase 7, apoptosis-related cysteine
protease
841308−1.3246996myosin, light polypeptide kinase
377461−1.3214357caveolin 1, caveolae protein, 22 kD
1473274−1.3200174myosin regulatory light chain 2, smooth
muscle isoform
1554167−1.3141843hypothetical protein FLJ14529
131839−1.3048208folate receptor 1 (adult)
67741−1.3014364PP2135 protein
132857−1.280579Homo sapiens mRNA; cDNA DKFZp586N1323
(from clone DKFZp586N1323)
345670−1.2805684ESTs, Moderately similar to I59348 CCAAT
binding transcription factor CBF subunit C -
rat [R. norvegicus]
781014−1.2659158suppression of tumorigenicity 5
289760−1.2610055ESTs, Highly similar to T00391 hypothetical
protein KIAA0612 [H. sapiens]
742685−1.248683disabled (Drosophila) homolog 2 (mitogen-
responsive phosphoprotein)
52419−1.2447753Friedreich ataxia region gene X123
838478−1.2349342neurocalcin delta
839736−1.2337192crystallin, alpha B
1558675−1.2313679SRY (sex determining region Y)-box 10
796181−1.2276581growth arrest-specific 6
878836−1.2200837secretory granule, neuroendocrine protein 1
(7B2 protein)
130201−1.2181641intercellular adhesion molecule 2
1917449−1.217371serum amyloid A4, constitutive
22917−1.2164705Homo sapiens mRNA; cDNA DKFZp761M0111
(from clone DKFZp761M0111)
1871116−1.209237Homo sapiens mRNA; cDNA DKFZp434C1714
(from clone DKFZp434C1714); partial cds
823871−1.2090693SPARC-like 1 (mast9, hevin)
811837−1.2030392
811848−1.2011809hypothetical protein
840266−1.200789Homo sapiens cDNA: FLJ22667 fis, clone
HSI08385
753071−1.2005994Homo sapiens cDNA: FLJ22528 fis, clone
HRC12825
2106144−1.1719133regulated in glioma
2504881−1.1674204signal transducer and activator of
transcription 5A
811837−1.157587eukaryotic translation elongation factor 1
alpha 1
486683−1.1548164Homo sapiens mRNA; cDNA DKFZp564J0323
(from clone DKFZp564J0323)
712139−1.1491129ADP-ribosylation factor-like 7
196435−1.1475545ESTs
877621−1.1422087nGAP-like protein
811088−1.140941ephrin-B3
322561−1.1270333ribosomal protein L31
712401−1.1218827phosphoinositide-3-kinase, catalytic, delta
polypeptide
788234−1.1061158inhibitor of DNA binding 4, dominant negative
helix-loop-helix protein
140574−1.103064small inducible cytokine subfamily D (Cys-X3-
Cys), member 1 (fractalkine, neurotactin)
490023−1.1020527hypothetical protein MGC2648
1584540−1.0933558Homo sapiens mRNA; cDNA DKFZp586M2022
(from clone DKFZp586M2022)
1556433−1.0887923GRO3 oncogene
47043−1.0841886tensin
303109−1.0807576purinergic receptor (family A group 5)
343760−1.0803279SH3 domain binding glutamic acid-rich protein
like 2
248631−1.0784377aminomethyltransferase (glycine cleavage
system protein T)
740620−1.071175tropomyosin 2 (beta)
529843−1.0698218ESTs, Moderately similar to JC5238
galactosylceramide-like protein, GCP
[H. sapiens]
990881−1.0640807
80344−1.0602825interleukin 7 receptor
625399−1.0594452hypothetical protein similar to beta-
transducin family
416676−1.0568729pellino (Drosophila) homolog 1
416959−1.0566462nuclear factor I/B
307029−1.0497874ribosomal protein L26
488404−1.0484323Homo sapiens clone TUA8 Cri-du-chat region
mRNA
291478−1.0400846runt-related transcription factor 3
153760−1.0340645EphB1
119290−1.025797cortic al thymocyte receptor (X. laevis CTX)
like
814443−1.0250673hypothetical protein MGC3232
757191−1.0238476ESTs
208718−1.0229324annexin A1
161456−1.0220494serum amyloid A1
1587710−1.0209983period (Drosophila) homolog 1
160609−1.0208819ESTs
593023−1.0158099dystrobrevin, beta
1878409−1.013872catechol-O-methyltransferase
781017−1.0125987early growth response 2 (Krox-20 (Drosophila)
homolog)
753162−1.0119485KIAA0603 gene product
897963−1.0075423phosphatidic acid phosphatase type 2A
505864−1.0033263RalGDS-like gene
323780−1.0027215Homo sapiens cDNA FLJ11177 fis, clone
PLACE1007402
343695−1.002495hypothetical protein FLJ10875
277571−0.9977509KIAA1706 protein
869450−0.9934083ribosomal protein L11
280907−0.9932806Kruppel-type zinc finger protein
290378−0.9932643podocalyxin-like
1635062−0.9905245DKFZP586A011 protein
270826−0.9896888Homo sapiens cDNA FLJ13329 fis, clone
OVARC1001795
1257131−0.9869928ESTs
|
Example VII
[0110] Genes for Discriminating Between ADH and DCIS
[0111] As shown in Table 6 below, 350 genes were identified as being able to discriminate between ADH and DCIS. The actual data is shown in FIG. 3.
6TABLE 6
|
|
CloneIDWeightDescription
|
|
1404774−0.5685596parathyroid hormone-like hormone
823871−0.9429443SPARC-like 1 (mast9, hevin)
1882697−1.4318896peanut (Drosophila)-like 2
140071−0.5708303frizzled-related protein
160192−1.1751869ESTs, Weakly similar to 2004399A chromosomal protein
[H. sapiens]
796542−1.2625621ets variant gene 5 (ets-related molecule)
611532−1.3158379troponin I, skeletal, fast
1473274−1.1172693myosin regulatory light chain 2, smooth muscle isoform
469306−0.5113377gastrin-releasing peptide
2306697−0.4494025neuromedin B
132857−1.0084069Homo sapiens mRNA; cDNA DKFZp586N1323 (from clone
DKFZp586N1323)
2504881−1.000863signal transducer and activator of transcription 5A
760299−1.7010462dickkopf (Xenopus laevis) homolog 3
293819−0.4051233oxidoreductase UCPA
1308350.3209345Homo sapiens, Similar to clone FLB3816, clone
IMAGE: 3454380, mRNA
66532−0.5773248endothelin 3
2499829−0.3619416zinc finger protein 145 (Kruppel-like, expressed in
promyelocytic leukemia)
85840−0.7754435nicotinamide N-methyltransferase
859359−0.4669761quinone oxidoreductase homolog
200814−1.4325283membrane metallo-endopeptidase (neutral endopeptidase,
enkephalinase, CALLA, CD10)
825287−0.2783909tumor necrosis factor (ligand) superfamily, member 11
202577−0.2283149histamine N-methyltransferase
20143730.0232775HNK-1 sulfotransferase
153760−0.9227865EphB1
377275−1.3102234ataxia-telangiectasia group D-associated protein
745490−0.6329354hypothetical protein FLJ20607
172783−0.6088873hypothetical protein FLJ10390
15581080.0044098ATP-binding cassette, sub-family C (CFTR/MRP), member 8
1587710−0.8252065period (Drosophila) homolog 1
141731−0.4531468
490484−0.3388325ESTs
504959−0.1620065Homo sapiens mRNA; cDNA DKFZp586G0321 (from clone
DKFZp586G0321)
1609746−0.4792809vitelliform macular dystrophy (Best disease, bestrophin)
882248−0.8700731transgelin
1917449−1.0894686serum amyloid A4, constitutive
2119838−0.3045374a disintegrin-like and metalloprotease (reprolysin type) with
thrombospondin type 1 motif, 8
8415070.0247736surfactant, pulmonary-associated protein A2
813265−1.2230435Homo sapiens mRNA; cDNA DKFZp564H1916 (from clone
DKFZp564H1916)
767202−1.0654145latent transforming growth factor beta binding protein 2
11565380.2252628potassium inwardly-rectifying channel, subfamily J, member
11
39600−0.6540275adenylate kinase 5
1630990−0.7312981ribosomal protein L29
41208−0.865227bone morphogenetic protein 1
322561−0.7970805ribosomal protein L31
951008−0.2221875ESTs
841308−0.9953716myosin, light polypeptide kinase
119290−0.8224756cortic al thymocyte receptor (X. laevis CTX) like
344959−0.0998837gene for serine/threonine protein kinase
810331−0.1389612quiescin Q6
1161775−0.7380353villin 1
625399−0.9066615hypothetical protein similar to beta-transducin family
1470657−0.3557985deiodinase, iodothyronine, type II
160609−0.8405669ESTs
665356−0.1575968tumor necrosis factor receptor superfamily, member 11b
(osteoprotegerin)
1584540−0.9754693Homo sapiens mRNA; cDNA DKFZp586M2022 (from clone
DKFZp586M2022)
726779−0.4459955calponin 1, basic, smooth muscle
296123−0.2814132Homo sapiens PRO1851 mRNA, complete cds
190059−0.634814guanine nucleotide binding protein (G protein), gamma 7
1471829−0.7275378uncharacterized hypothalamus protein HSMNP1
2056139−1.0673271LIM domain protein
190753−0.4423669ESTs
1968422−0.5802058Homo sapiens mRNA full length insert cDNA clone
EUROIMAGE 1968422
725390−0.4620278glutathione S-transferase pi
814826−0.8443265ESTs
878836−1.037352secretory granule, neuroendocrine protein 1 (7B2 protein)
27769−0.8298821ESTs
188388−0.6824191integrin, alpha 10
1597813−0.5406089hypothetical protein PP1044
1609625−0.5920312selectin P ligand
810981−0.1805871hypothetical protein FLJ20699
726703−0.070084Homo sapiens clone 23736 mRNA sequence
781014−1.0161379suppression of tumorigenicity 5
8982220.1795078Homo sapiens clone 24418 mRNA sequence
1475738−0.6753091ribosomal protein S25
1492144−0.2356446butyrophilin, subfamily 3, member A2
1492147−0.636656ribosomal protein S4, X-linked
1711456−0.4896574H factor (complement)-like 1
1871116−0.9821709Homo sapiens mRNA; cDNA DKFZp434C1714 (from clone
DKFZp434C1714); partial cds
344720−1.5196431glycophorin C (Gerbich blood group)
45099−1.3515907regucalcin (senescence marker protein-30)
307029−0.6500872ribosomal protein L26
25763−0.2725443ankylosis, progressive (mouse) homolog
2502722−0.3315871loss of heterozygosity, 11, chromosomal region 2, gene A
1665444−0.651994tumor endothelial marker 1 precursor
165837−0.3443143translocase of inner mitochondrial membrane 22 (yeast)
homolog
502518−0.4342228laminin, beta 2 (laminin S)
897963−0.9775616phosphatidic acid phosphatase type 2A
1201380.1570107J domain containing protein 1
78946−0.648366Homo sapiens pyruvate dehydrogenase kinase 4 mRNA, 3′
untranslated region, partial sequence
990881−0.8335353
859192−0.2265317vesicle-associated membrane protein 1 (synaptobrevin 1)
712023−0.5302372AT-binding transcription factor 1
855586−0.3944697nuclear receptor subfamily 3, group C, member 1
52419−1.0368509Friedreich ataxia region gene X123
432072−0.4193898nuclear factor of activated T-cells, cytoplasmic, calcineurin-
dependent 1
1897947−0.163146surfactant, pulmonary-associated protein A2
727229−0.2668972mitogen-activated protein kinase kinase kinase 4
810358−0.5403705acyl-Coenzyme A dehydrogenase, very long chain
154600−0.0683999phospholipase C, delta 1
755855−0.6298333solute carrier family 25 (mitochondrial carrier; adenine
nucleotide translocator), member 6
323780−0.8879854Homo sapiens cDNA FLJ11177 fis, clone PLACE1007402
230560−0.1803944ESTs
283173−0.4293696EBP50-PDZ interactor of 64 kD
265853−0.3519023Homo sapiens cDNA FLJ11298 fis, clone PLACE1009794
161456−0.8215502serum amyloid A1
184022−0.7070266amyloid beta (A4) precursor protein-binding, family B,
member 1 (Fe65)
1636523−0.1496249glutathione S-transferase subunit 13 homolog
795730−0.2892776signal transduction protein (SH3 containing)
1631682−0.5162406peptidylprolyl isomerase E (cyclophilin E)
811837−0.848137
854763−0.0493902Homo sapiens cDNA FLJ11341 fis, clone PLACE1010786
50562−0.0943363chromosome 8 open reading frame 4
46843−0.1736687neuronal Shc adaptor homolog
178137−0.6839022ribosomal protein L34
1662279−0.2290724plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
1635062−0.8243024DKFZP586A011 protein
293916−0.3671832FKBP-associated protein
415613−0.5528761DHHC1 protein
80344−0.8243418interleukin 7 receptor
1602798−0.4300808choline kinase-like
1910516−0.2845842ESTs
740620−0.96496tropomyosin 2 (beta)
277627−0.5329107Human SH3 domain-containing protein SH3P18 mRNA,
complete cds
8546960.008949siah binding protein 1; FBP interacting represser; pyrimidine
tract binding splicing factor; Ro ribonucleoprotein-binding
protein 1
45578−0.1295485mitogen-activated protein kinase kinase 6
950710−0.346361propionyl Coenzyme A carboxylase, alpha polypeptide
768043−0.4005598ECSIT
308539−0.6726187Homo sapiens cDNA FLJ12777 fis, clone NT2RP2001720
595637−0.5373697Homo sapiens mRNA; cDNA DKFZp586N012 (from clone
DKFZp586N012)
143661−0.4713778netrin 4
248631−0.9106505aminomethyltransferase (glycine cleavage system protein T)
8266220.1339287KIAA0430 gene product
51981−0.5554908ribosomal protein L7a
811848−1.1350073hypothetical protein
1533710−0.5988282ortholog of mouse integral membrane glycoprotein LIG-1
2017756−0.2549438homolog of yeast MOG1
1637296−0.5105214ribosomal protein S24
50586−0.6794282KIAA1545 protein
877835−0.5382125ribosomal protein L35
773319−0.285447ribosomal protein S6 kinase, 70 kD, polypeptide 1
2014888−0.2871529sushi-repeat protein
767495−0.0366213GLI-Kruppel family member GLI3 (Greig
cephalopolysyndactyly syndrome)
774078−0.6508021leiomodin 1 (smooth muscle)
868400−0.4928479glutaminyl-tRNA synthetase
240620−0.5065007vascular Rab-GAP/TBC-containing
1588791−0.1606643O-6-methylguanine-DNA methyltransferase
472186−0.2687871RAB32, member RAS oncogene family
725143−0.3410957hypothetical protein FLJ22418
714472−0.3677387KIAA0397 gene product
18546480.0089186hemopexin
8550290.076972Ac-like transposable element
197727−0.3906866phosphatidylethanolamine N-methyltransferase
1623016−0.6263561EST
8138410.0885625plasminogen activator, tissue
2783721−0.6854649cholinergic receptor, nicotinic, beta polypeptide 1 (muscle)
549933−0.3547881interleukin 8
280907−0.7307519Kruppel-type zinc finger protein
796181−0.8888496growth arrest-specific 6
415233−0.6506593ribosomal protein L37a
811920−1.2690713interleukin 11 receptor, alpha
415415−0.646492EST, Moderately similar to RL1X_HUMAN 60S RIBOSOMAL
PROTEIN L18A [H. sapiens]
2018807−0.2989936KIAA0468 gene product
154999−0.3876757hypothetical protein FLJ21007
685570.0637586fatty acid binding protein 1, liver
647763−0.1436566ESTs
1568967−0.2938468ESTs
210717−0.4522432syndecan 2 (heparan sulfate proteoglycan 1, cell surface-
associated, fibroglycan)
1350439−0.6740833KIAA0015 gene product
684582−0.0089016tryptophanyl tRNA synthetase 2 (mitochondrial)
122394−0.049399guanine nucleotide binding protein (G protein), alpha 15 (Gq
class)
213136−0.1139004BTG family, member 2
625458−0.2394796hypothetical protein MGC3234
811162−0.3821116fibromodulin
1926246−0.701769ESTs, Moderately similar to T46371 hypothetical protein
DKFZp434P1018.1 [H. sapiens]
1554167−1.0818834hypothetical protein FLJ14529
811088−1.0851546ephrin-B3
72778−1.1752838caspase 7, apoptosis-related cysteine protease
220293−0.5078291Homo sapiens cDNA: FLJ21800 fis, clone HEP00618
1569187−1.2971252heparan sulfate (glucosamine) 3-O-sulfotransferase 4
866866−0.4801351Ras association (RalGDS/AF-6) domain family 1
81316−0.6176265ESTs
1161564−1.3891189desmuslin
898044−0.4691863metallocarboxypeptidase CPX-1
293001−0.4472585hypothetical protein DKFZp434E2318
1570502−0.3314609Homo sapiens cDNA FLJ12936 fis, clone NT2RP2005018
839796−0.6807912candidate tumor suppressor p33 ING1 homolog
380883−0.4438655Homo sapiens cDNA FLJ10158 fis, clone HEMBA1003463
180561−0.3250633glutathione S-transferase M4
869450−0.7659922ribosomal protein L11
47043−0.7729643tensin
810463−0.0829995DKFZP566O084 protein
1495390.4138942KIAA1700
200354−0.0613983thymidine kinase 2, mitochondrial
7711730.1757755mitochondrial ribosomal protein S21
270826−0.9389651Homo sapiens cDNA FLJ13329 fis, clone OVARC1001795
810017−0.1076821plasminogen activator, urokinase receptor
8098380.0956351
344168−0.386185polymerase (DNA directed), lambda
785967−0.6049171erythrocyte membrane protein band 4.1-like 2
511831−0.2912672hypothetical protein MGC12936
282404−0.5528532Homo sapiens mRNA for K1AA1671 protein, partial cds
730036−0.079736Mad4 homolog
298231−0.3550893gamma-aminobutyric acid (GABA) B receptor, 1
2106144−1.067765regulated in glioma
743880−0.3262292KIAA0263 gene product
268234−0.1535073Dmx-like 1
280776−0.2922639acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
212078−0.1831462integrin, alpha 1
7313080.5371204citrate synthase
774471−0.1753902laminin, beta 1
811837−0.8764231eukaryotic translation elongation factor 1 alpha 1
7144370.0258281sema domain, immunoglobulin domain (Ig), transmembrane
domain (TM) and short cytoplasmic domain, (semaphorin) 4B
416959−0.957248nuclear factor I/B
681992−0.7691299Homo sapiens cDNA FLJ13384 fis, clone PLACE1001062,
highly similar to Homo sapiens mRNA for lysine-
ketoglutarate reductase/saccharopine dehydrogenase
130201−1.0401997intercellular adhesion molecule 2
3631440.1937891transcription factor AP-2 beta (activating enhancer-binding
protein 2 beta)
857874−0.3703645transforming growth factor beta-activated kinase-binding
protein 1
377461−1.2798035caveolin 1, caveolae protein, 22 kD
1563630.2685238hypothetical protein FLJ12934
589115−0.0115547matrix metalloproteinase 1 (interstitial collagenase)
241489−0.6212938adrenergic, beta-2-, receptor, surface
5867250.0683397protein phosphatase 2, regulatory subunit B (B56), beta
isoform
283124−0.7933772ESTs, Moderately similar to LONG-CHAIN FATTY ACID
TRANSPORT PROTEIN [M. musculus]
307328−0.4022704hypothetical protein FLJ10948
37449−0.3539978GAS2-related on chromosome 22
50892−0.6887497Homo sapiens, clone MGC: 9913 IMAGE: 3870821, mRNA,
complete cds
1897944−0.4855156ESTs, Weakly similar to T00366 hypothetical protein
KIAA0669 [H. sapiens]
377314−0.561312casein kinase 2, alpha prime polypeptide
220851−0.0351418crystallin, alpha A
322233−0.3025167
586803−0.7423407placental growth factor, vascular endothelial growth factor-
related protein
343695−0.7706809hypothetical protein FLJ10875
414999−0.5002542ets variant gene 4 (E1A enhancer-binding protein, E1AF)
813823−0.4463267lumican
178825−0.1525189neurogranin (protein kinase C substrate, RC3)
85582−0.0716296target of myb1 (chicken) homolog-like 2
753620−0.5248489insulin-like growth factor binding protein 6
1899338−0.066168mannosidase, alpha, class 1A, member 2
3592500.0674486carbonic anhydrase IV
345034−1.0600859small inducible cytokine subfamily B (Cys-X-Cys), member
14 (BRAK)
2164744−0.2307289neural cell adhesion molecule 1
613056−0.3957342reticulocalbin 1, EF-hand calcium binding domain
1410444−0.0368027amphiregulin (schwannoma-derived growth factor)
593023−0.7516846dystrobrevin, beta
7885110.1403148ribosomal protein S6 kinase, 90 kD, polypeptide 1
4552690.0223607
1650927−0.1712163hypothetical protein DKFZp547E052
782427−0.2818886inhibin, beta B (activin AB beta polypeptide)
71727−0.2796517T-cell acute lymphocytic leukemia 1
840942−0.6383764major histocompatibility complex, class II, DP beta 1
8254700.5218731topoisomerase (DNA) II alpha (170 kD)
5952130.4824381hypothetical protein
7966940.6893307baculoviral IAP repeat-containing 5 (survivin)
8107110.9128832stearoyl-CoA desaturase (delta-9-desaturase)
6299440.5273447myosin VB
664060.5966701hypothetical protein DKFZp762E1312
7858400.5401209SEC24 (S. cerevisiae) related gene family, member D
2108620.4683996acyl-Coenzyme A oxidase 1, palmitoyl
842950.3989864interleukin 1 receptor antagonist
4291820.2952867dolichyl-phosphate mannosyltransferase polypeptide 1,
catalytic subunit
7050640.4497555transforming, acidic coiled-coil containing protein 3
7825130.4759571interferon, alpha-inducible protein (clone IFI-6-16)
8396820.5208954ubiquitin-conjugating enzyme E2N (homologous to yeast
UBC13)
7683770.3809674activity-dependent neuroprotective protein
23090730.5226599frizzled (Drosophila) homolog 5
7893760.4339171thioredoxin reductase 1
196992−0.1015205aldo-keto reductase family 1, member C1 (dihydrodiol
dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid
dehydrogenase)
7857070.4708376protein regulator of cytokinesis 1
15050381.1904802hypothetical protein FLJ20171
8693750.4827093isocitrate dehydrogenase 2 (NADP+), mitochondrial
1092210.3048287KIAA0286 protein
3457870.4647372highly expressed in cancer, rich in leucine heptad repeats
462480.640944ADP-ribosyltransferase (NAD+; poly (ADP-ribose)
polymerase)
795200.6769129RAB2, member RAS oncogene family
4693830.5558732chromosome 8 open reading frame 1
5095880.4157059TATA box binding protein (TBP)-associated factor, RNA
polymerase II, J, 20 kD
2929360.4217115hypothetical protein FLJ10468
6861720.6724684KIAA0008 gene product
7886550.2976191HTPAP protein
7709920.3331315
15878470.1732392minichromosome maintenance deficient (mis5, S. pombe) 6
797100.4579008KIAA0174 gene product
20565660.2341905integrin, alpha M (complement component receptor 3, alpha;
also known as CD11b (p170), macrophage antigen alpha
polypeptide)
4543390.3738929thiopurine S-methyltransferase
5942260.403676Homo sapiens cDNA FLJ14459 fis, clone HEMBB1002409
8978130.4749348polyadenylate binding protein-interacting protein 1
8246940.0268936protein tyrosine phosphatase type IVA, member 1
7254540.6669973CDC28 protein kinase 2
792540.0732432MHC class I region ORF
14727190.2550065SMT3 (suppressor of mif two 3, yeast) homolog 1
20546350.7914755proteasome (prosome, macropain) subunit, alpha type, 7
2899780.6308861ubiquitin-like 4
1558060.27471012′-5′-oligoadenylate synthetase 2 (69-71 kD)
1478340.4412795zinc finger protein 217
261710.3656147KIAA0856 protein
23223670.5832711reticulon 4
7699210.6464245ubiquitin carrier protein E2-C
730090.4822587ESTs, Weakly similar to A43932 mucin 2 precursor, intestinal
[H. sapiens]
20140340.6620303methylene tetrahydrofolate dehydrogenase (NAD+
dependent), methenyltetrahydrofolate cyclohydrolase
3598870.8692353translocase of inner mitochondrial membrane 17 (yeast)
homolog A
4889640.9569176H2A histone family, member O
14760650.289904stathmin 1/oncoprotein 18
14223380.5874097ribonucleotide reductase M2 polypeptide
2689460.5440003WD40 protein Ciao1
6865520.7231319golgi phosphoprotein 1
1495440.283642neuroepithelial cell transforming gene 1
7706750.454729Homo sapiens cDNA: FLJ21323 fis, clone COL02374
3462570.1515899minichromosome maintenance deficient (S. cerevisiae) 4
4297990.6915155hypothetical protein FLJ21939 similar to 5-azacytidine
induced gene 2
1439970.1883447proteasome (prosome, macropain) 26S subunit, non-
ATPase, 10
1222410.7394284proteasome (prosome, macropain) subunit, beta type, 2
8235980.9153521proteasome (prosome, macropain) 26S subunit, non-
ATPase, 12
8146320.0960611splicing factor, arginine/serine-rich (transformer 2 Drosophila
homolog) 10
8103160.3732635very long-chain acyl-CoA synthetase; lipidosin
2908410.8346933H2B histone family, member A
3473730.7563599transcription elongation factor B (SIII), polypeptide 1 (15 kD,
elongin C)
7007920.4949149cyclin-dependent kinase inhibitor 3 (CDK2-associated dual
specificity phosphatase)
15545490.9764206hydroxyacyl glutathione hydrolase
8977700.4270685
5043080.5564295hypothetical protein FLJ10540
1328280.2875519Down syndrome critical region gene 1-like 2
2923880.112577
16162530.567837breast carcinoma amplified sequence 1
7964691.0269115HSPC150 protein similar to ubiquitin-conjugating enzyme
2139152−0.4373776Homo sapiens clone 24473 mRNA sequence
1302760.2154295Homo sapiens mRNA; cDNA DKFZp586H0324 (from clone
DKFZp586H0324)
341490.309569KIAA0227 protein
1500030.8251408hypothetical protein FLJ13187
2877490.3173706CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1
253800.164077ESTs, Moderately similar to JC5238 galactosylceramide-like
protein, GCP [H. sapiens]
7450830.4107735ubiquitin specific protease 18
8108990.240336CDC28 protein kinase 1
7680590.3139886hypothetical protein FLJ12619
8150260.4472842cleavage stimulation factor, 3′ pre-RNA, subunit 3, 77 kD
7414740.7732047glucose phosphate isomerase
7886410.3638059adaptor-related protein complex 1, sigma 2 subunit
20431670.7167431BCL2-associated athanogene 3
14927800.6544659Homo sapiens cDNA FLJ14459 fis, clone HEMBB1002409
23069870.4402912secreted and transmembrane 1
7546530.4405873cadherin, EGF LAG seven-pass G-type receptor 3, flamingo
(Drosophila) homolog
7438100.3667087hypothetical protein MGC2577
1268580.2389814sterol O-acyltransferase (acyl-Coenzyme A: cholesterol
acyltransferase) 1
20174150.43546centromere protein A (17 kD)
2805070.2846518hypoxanthine phosphoribosyltransferase 1 (Lesch-Nyhan
syndrome)
672370.1406869ESTs
43833−0.1197546diacylglycerol kinase, gamma (90 kD)
420760.3194462TRK-fused gene
5313190.1093932serine/threonine kinase 12
7841290.2902598tyrosine 3-monooxygenase/tryptophan 5-monooxygenase
activation protein, gamma polypeptide
5868950.2549108small nuclear ribonucleoprotein polypeptide G
|
Example VIII
[0112] Genes for Discriminating Between DCIS Grade I and Grade III
[0113] As shown in Table 7 below, 350 genes were identified as being able to discriminate between grades of DCIS. The actual data corresponding to this table is shown in FIG. 4.
7TABLE 7
|
|
CloneIDWeightDescription
|
|
24601594.6268975tyrosine kinase, non-receptor, 1
3581512.8783989zinc finger protein 33a (KOX 31)
7953822.772572Rap1 guanine-nucleotide-exchange factor directly activated by
cAMP
7144722.6829714KIAA0397 gene product
7256492.6433625nuclear factor of activated T-cells, cytoplasmic, calcineurin-
dependent 4
512182.6200167ESTs
5049592.5777963Homo sapiens mRNA; cDNA DKFZp586G0321 (from clone
DKFZp586G0321)
6473972.5320153ESTs
8148152.4198892plakophilin 4
2797202.4136316ESTs, Moderately similar to A47582 B-cell growth factor
precursor [H. sapiens]
2982312.4096172gamma-aminobutyric acid (GABA) B receptor, 1
1727832.3339699hypothetical protein FLJ10390
2616092.311051ESTs, Weakly similar to S65657 alpha-1C-adrenergic receptor
splice form 2 [H. sapiens]
8266682.2951137KIAA0274 gene product
14933832.2616737Homo sapiens mRNA; cDNA DKFZp434H2418 (from clone
DKFZp434H2418)
20177562.2403622homolog of yeast MOG1
14555662.1756946adenosine A3 receptor
7253212.1658095estrogen receptor 1
1805612.1393944glutathione S-transferase M4
320502.1165022Homo sapiens mRNA; cDNA DKFZp586P1124 (from clone
DKFZp586P1124)
2150002.1150123vasoactive intestinal peptide receptor 1
20197502.0957741SEC14 (S. cerevisiae)-like 2
2831242.0921779ESTs, Moderately similar to LONG-CHAIN FATTY ACID
TRANSPORT PROTEIN [M. musculus]
4906152.0635424tubulin, gamma 2
6661382.0625667hypothetical protein DKFZp761J1523
4181292.0541212nuclear mitotic apparatus protein 1
17332622.052666BLu protein
15887912.044069O-6-methylguanine-DNA methyltransferase
4617612.035953angiogenin, ribonuclease, RNase A family, 5
10315921.9930012kinesin protein 9 gene
1264151.9781057Homo sapiens mRNA; cDNA DKFZp566H0124 (from clone
DKFZp566H0124)
286431.9733043hypothetical protein DKFZp564D1378
4702611.9160486
16309901.9155421ribosomal protein L29
8109811.9132205hypothetical protein FLJ20699
7674951.9052671GLI-Kruppel family member GLI3 (Greig
cephalopolysyndactyly syndrome)
7671761.8996792tumor necrosis factor (ligand) superfamily, member 13
15721961.8855996secreted modular calcium-binding protein 2
17066351.8754346bone gamma-carboxyglutamate (gla) protein (osteocalcin)
1863011.8424458Homo sapiens cDNA FLJ12924 fis, clone NT2RP2004709
7267031.842399Homo sapiens clone 23736 mRNA sequence
2142051.8416657Homo sapiens, clone MGC: 17687 IMAGE: 3865868, mRNA,
complete cds
7841781.822159Homo sapiens mRNA; cDNA DKFZp586M0723 (from clone
DKFZp586M0723)
3469021.8196723ESTs, Weakly similar to ALU2_HUMAN ALU SUBFAMILY SB
SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
13676781.8184255KIAA0356 gene product
1900591.7988757guanine nucleotide binding protein (G protein), gamma 7
14569371.7980645oviductal glycoprotein 1, 120 kD (mucin 9, oviductin)
455781.7935215mitogen-activated protein kinase kinase 6
2486311.7701919aminomethyltransferase (glycine cleavage system protein T)
15622311.7668811SET binding protein 1
1544661.7516237STIP1 homology and U-Box containing protein 1
25244451.7392197neuronal PAS domain protein 1
2772661.7307752Homo sapiens, clone IMAGE: 3625550, mRNA, partial cds
7418911.7297992RAB2, member RAS oncogene family-like
2062171.7227658nuclear receptor subfamily 1, group H, member 3
20288761.7128178splicing factor, arginine/serine-rich 5
7300361.70573Mad4 homolog
15582331.7035129ESTs
5025181.7027287laminin, beta 2 (laminin S)
3568351.7014349hypothetical protein MGC10500
7449941.6938348hypothetical protein FLJ12242
8103581.6924263acyl-Coenzyme A dehydrogenase, very long chain
8138541.6914133purine-rich element binding protein A
7680431.6854123ECSIT
2646321.6591646ESTs
5052431.6581518inositol 1,4,5-triphosphate receptor, type 2
3440731.6571604ESTs, Weakly similar to K1CI_HUMAN KERATIN, TYPE I
CYTOSKELETAL 9 [H. sapiens]
3437601.6435915SH3 domain binding glutamic acid-rich protein like 2
15694181.6408767Homo sapiens cDNA FLJ11385 fis, clone HEMBA1000520
2025771.6393518histamine N-methyltransferase
8236341.6366047ESTs
8397961.630887candidate tumor suppressor p33 ING1 homolog
1834401.6297915arylsulfatase A
3449591.6268043gene for serine/threonine protein kinase
15742521.6211224DKFZP586D0623 protein
6693591.6174855Homo sapiens clone 24405 mRNA sequence
740701.6096611endosulfine alpha
16606491.6051582suppressor of white apricot homolog 2
7967231.6041219Homo sapiens clone CDABP0014 mRNA sequence
7431461.597094hypothetical protein FLJ23403
7891471.5937127enolase 2, (gamma, neuronal)
3421811.5926355B-cell CLL/lymphoma 2
330761.5824352cholinephosphotransferase 1
7824971.5804781Homo sapiens, clone IMAGE: 3010666, mRNA, partial cds
4702611.5751564SMA5
8095071.5723669hypothetical protein FLJ20568
7124601.5721924natural killer-tumor recognition sequence
2935691.5662317chromosome 1 open reading frame 21
15184021.5627158KIAA1361 protein
1550721.5557559ESTs
4552691.5538593
2628041.5532348hypothetical protein MGC2941
14922381.5484044HSPC003 protein
3648651.5483958hypothetical protein FLJ21062
23258041.546182395 kDa retinoblastoma protein binding protein
16350621.5449944DKFZP586A011 protein
3441681.5421405polymerase (DNA directed), lambda
15171711.5381921interleukin 2 receptor, alpha
7696001.5335878uracil-DNA glycosylase 2
3255831.5245817EST
8148261.5223296ESTs
15705021.5184543Homo sapiens cDNA FLJ12936 fis, clone NT2RP2005018
5118311.5183298hypothetical protein MGC12936
1249221.5052004KRAB-zinc finger protein SZF1-1
20727681.4886791nuclear receptor coactivator 3
20218821.4854251sodium channel, nonvoltage-gated 1, delta
6272481.4827018SBBI31 protein
7255031.476461D-dopachrome tautomerase
2853121.4699425
1417311.469088
14567011.4668054B-cell CLL/lymphoma 9
8982221.4667947Homo sapiens clone 24418 mRNA sequence
7252841.4638006phosphorylase kinase, gamma 2 (testis)
1549991.4564204hypothetical protein FLJ21007
15925301.4558873mammalian inositol hexakisphosphate kinase 2
5903101.4534138Homo sapiens mRNA; cDNA DKFZp434E2321 (from clone
DKFZp434E2321); partial cds
4160421.446691ESTs, Weakly similar to ALU1_HUMAN ALU SUBFAMILY J
SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
20171441.4450059CGI-41 protein
3094491.4412699ribosomal protein S4, Y-linked
4503011.4403678mutL (E. coli) homolog 3
14924681.4375076DEME-6 protein
1214541.4361788arachidonate 12-lipoxygenase
7438801.4294838KIAA0263 gene product
15689671.4109448ESTs
15689891.4094472ESTs
252741.4078252g20 protein
24133371.4058288sortilin-related receptor, L(DLR class) A repeats-containing
1979131.3994468splicing factor proline/glutamine rich (polypyrimidine tract-
binding protein-associated)
2927701.3952057Homo sapiens, clone IMAGE: 3627860, mRNA, partial cds
1433321.3875805neuropeptide Y receptor Y1
8097791.3848708KIAA0239 protein
1382421.3820433ESTs, Moderately similar to MAS2_HUMAN MANNAN-
BINDING LECTIN SERINE PROTEASE 2 PRECURSOR
[H. sapiens]
2701271.3786197
492401.3772023KIAA0460 protein
8266221.3734143KIAA0430 gene product
18588371.3719341ESTs
15831981.3712112ESTs, Weakly similar to S65824 reverse transcriptase
homolog [H. sapiens]
3458581.3629187cisplatin resistance associated
2083871.3597205KIAA1407 protein
5027821.3589088RAN binding protein 3
262941.3578289RNB6
6693791.3559931Homo sapiens BAC clone RP11-505D17 from 7p22-p21
8107281.355262hypothetical gene ZD52F10
16018451.3533864Ca2+-promoted Ras inactivator
8408821.350538nucleotide binding protein
821731.3485351MYLE protein
4909651.3476943ESTs
8111621.3450626fibromodulin
610611.3437368hypothetical protein FLJ20585
496301.3412751calcium channel, voltage-dependent, L type, alpha 1D subunit
825659−2.8345933N-myc downstream regulated
769921−2.712812ubiquitin carrier protein E2-C
788232−2.6550962sestrin 2
1476053−2.529039RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
809557−2.4910488minichromosome maintenance deficient (S. cerevisiae) 3
150897−2.4302462UDP-GIcNAc: betaGal beta-1,3-N-
acetylglucosaminyltransferase 3
814526−2.37273seb4D
796694−2.3104815baculoviral IAP repeat-containing 5 (survivin)
814792−2.3001334ubiquitin specific protease 10
1536236−2.2971152hypothetical protein FLJ13154
2017415−2.2748559centromere protein A (17 kD)
210862−2.2408114acyl-Coenzyme A oxidase 1, palmitoyl
795543−2.2403389thioredoxin peroxidase (antioxidant enzyme)
773301−2.2239527cadherin 3, type 1, P-cadherin (placental)
700792−2.2203557cyclin-dependent kinase inhibitor 3 (CDK2-associated dual
specificity phosphatase)
1883327−2.2093248ESTs
1523225−2.1936598oncostatin M receptor
208718−2.1882654annexin A1
1702742−2.1567809solute carrier family 7 (cationic amino acid transporter, y+
system), member 5
753378−2.151145hypothetical protein FLJ22649 similar to signal peptidase
SPC22/23
429222−2.1351022CGI-107 protein
292388−2.1343367
781047−2.1262682budding uninhibited by benzimidazoles 1 (yeast homolog)
450854−2.0819596craniofacial development protein 1
823598−2.0693353proteasome (prosome, macropain) 26S subunit, non-ATPase,
12
1422338−2.0689327ribonucleotide reductase M2 polypeptide
705064−2.0601901transforming, acidic coiled-coil containing protein 3
770675−2.0272207Homo sapiens cDNA: FLJ21323 fis, clone COL02374
345787−1.9992897highly expressed in cancer, rich in leucine heptad repeats
471196−1.9985751integral membrane protein 3
753215−1.9954476guanine nucleotide binding protein (G protein), alpha inhibiting
activity polypeptide 1
869375−1.9803724isocitrate dehydrogenase 2 (NADP+), mitochondrial
842818−1.9796507lysyl-tRNA synthetase
229579−1.9782493Golgi apparatus protein 1
1916461−1.9736332hypothetical protein
129294−1.9730334ESTs
789376−1.9719575thioredoxin reductase 1
1897302−1.9681938serine/threonine kinase 17b (apoptosis-inducing)
951117−1.9577807eukaryotic translation elongation factor 1 gamma
1642496−1.9484342hypothetical protein MGC11266
591465−1.9277062Homo sapiens, clone MGC: 2908 IMAGE: 3029644, mRNA,
complete cds
149355−1.8999907translocating chain-associating membrane protein
259950−1.8958779hypothetical protein FLJ14991
897770−1.8887034
878798−1.8774101beta-2-microglobulin
746229−1.873434mitogen-activated protein kinase kinnse kinase kinase 4
624867−1.8594915hypothetical protein FLJ20186
504308−1.8590049hypothetical protein FLJ10540
727251−1.8553757CD9 antigen (p24)
897774−1.8532125adenine phosphoribosyltransferase
1901310−1.8434048KIAA1209 protein
292936−1.838542hypothetical protein FLJ10468
1518591−1.8382765
321354−1.8123042mitochondrial ribosomal protein L15
1903066−1.8071117keratin, hair, basic, 1
235180−1.8004039ESTs, Moderately similar to ALU1_HUMAN ALU SUBFAMILY
J SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
740604−1.7995578interferon stimulated gene (20 kD)
233464−1.7978669epithelial V-like antigen 1
951241−1.7961406clone HQ0310 PRO0310p1
1587847−1.7886092minichromosome maintenance deficient (mis5, S. pombe) 6
624390−1.7800152DC13 protein
128711−1.7791673anillin (Drosophila Scraps homolog), actin binding protein
308633−1.7667341hypothetical protein FLJ10339
884425−1.761486chaperonin containing TCP1, subunit 5 (epsilon)
745394−1.7583344Homo sapiens cDNA: FLJ23249 fis, clone COL04196
852829−1.7579704karyopherin alpha 3 (importin alpha 4)
122241−1.7490794proteasome (prosome, macropain) subunit, beta type, 2
307255−1.7294781basement membrane-induced gene
32493−1.7273675integrin, alpha 6
454896−1.7216328DnaJ (Hsp40) homolog, subfamily A, member 2
1876217−1.7186822DnaJ (Hsp40) homolog, subfamily A, member 2
815556−1.7161709hypothetical protein FLJ10430
589869−1.7120206transcriptional co-activator with PDZ-binding motif (TAZ)
2012523−1.7104314fatty acid binding protein 5 (psoriasis-associated)
1946448−1.7092537caveolin 2
841370−1.7047297glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate
aminotransferase 2)
1600239−1.6989076HSPC037 protein
462926−1.6981004NIMA (never in mitosis gene a)-related kinase 2
144880−1.6980733hypothetical protein from EUROIMAGE 1759349
744047−1.6910803polo (Drosophia)-like kinase
624627−1.6888444ribonucleotide reductase M2 polypeptide
788566−1.6752673Purkinje cell protein 4
858293−1.6732706tyrosine 3-monooxygenase/tryptophan 5-monooxygenase
activation protein, theta polypeptide
470148−1.659943ESTs
66902−1.6546363ESTs
77533−1.6505418inositol polyphosphate-5-phosphatase, 40 kD
825282−1.6417354DKFZP586L0724 protein
785840−1.6406565SEC24 (S. cerevisiae) related gene family, member D
824962−1.6399941karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
741139−1.6396499eyes absent (Drosophila) homolog 2
809530−1.6264151minichromosome maintenance deficient (S. cerevisiae) 2
(mitotin)
2054635−1.6254343proteasome (prosome, macropain) subunit, alpha type, 7
813533−1.6215197syndecan binding protein (syntenin)
267816−1.6173276KIAA0551 protein
306318−1.6083862origin recognition complex, subunit 6 (yeast homolog)-like
796469−1.6065509HSPC150 protein similar to ubiquitin-conjugating enzyme
813256−1.6027042ATP-binding cassette, sub-family B (MDR/TAP), member 1
843121−1.60128chloride intracellular channel 1
713685−1.6006729protease, serine, 2 (trypsin 2)
811590−1.6001102hypothetical protein FLJ11100
823756−1.5995448mitogen inducible 2
549073−1.597146capping protein (actin filament) muscle Z-line, alpha 2
743810−1.5960999hypothetical protein MGC2577
377368−1.5955102cell death regulator aven
1506046−1.5918448hypothetical protein FLJ10815
814270−1.5890389polymyositis/scleroderma autoantigen 1 (75 kD)
1604703−1.5853167major histocompatibility complex, class I, F
234736−1.5765159GATA-binding protein 6
429182−1.5699689dolichyl-phosphate mannosyltransferase polypeptide 1,
catalytic subunit
826355−1.5677013vesicle-associated membrane protein 5 (myobrevin)
34149−1.5659923KIAA0227 protein
746190−1.5635857hypothetical protein DKFZp761B1514
131091−1.558448Not56 (D. melanogaster)-like protein
665384−1.5550415KIAA1609 protein
625683−1.5501423neighbor of COX4
1582738−1.544427uncharacterized bone marrow protein BM040
949988−1.5437326Homo sapiens mRNA; cDNA DKFZp586E1124 (from clone
DKFZp586E1124); complete cds
133213−1.5433392fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-
specific)
1660666−1.5423907carbonic anhydrase VB, mitochondrial
713158−1.5372729ESTs
1614140−1.5341302Ris
201890−1.5333626baculoviral IAP repeat-containing 3
38925−1.5304604ESTs, Moderately similar to A47582 B-cell growth factor
precursor [H. sapiens]
511850−1.5294752proteasome (prosome, macropain) 26S subunit, ATPase, 1
489489−1.5169076lamin B receptor
825470−1.5154306topoisomerase (DNA) II alpha (170 kD)
42831−1.5136459N-terminal kinase-like
809784−1.5066053kallikrein 6 (neurosin, zyme)
730410−1.506168lymphocyte-specific protein tyrosine kinase
810983−1.5060386DKFZP434H132 protein
731223−1.5058799proteasome (prosome, macropain) subunit, beta type, 2
259017−1.5053827ESTs
340745−1.5041978ESTs
746163−1.5000358ESTs, Weakly similar to ALU1_HUMAN ALU SUBFAMILY J
SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
1466621−1.4998321ATPase, Ca++ transporting, type 2C, member 1
809588−1.49754gamma-glutamyl hydrolase (conjugase,
folylpolygammaglutamyl hydrolase)
813707−1.4946872regulator of G-protein signalling 16
188335−1.4895328egf-like module containing, mucin-like, hormone receptor-like
sequence 2
1493160−1.4851884small inducible cytokine subfamily B (Cys-X-Cys), member 10
531886−1.4842053Homo sapiens clone FLB3344 PRO0845 mRNA, complete cds
753428−1.4835152Homo sapiens, clone IMAGE: 3542597, mRNA, partial cds
897731−1.4833877latrophilin
376551−1.4811859ETAA16 protein
256907−1.4755682glutathione S-transferase A3
712139−1.4735772ADP-ribosylation factor-like 7
785368−1.4680554PDZ-binding kinase; T-cell originated protein kinase
825606−1.4667088kinesin-like 1
531319−1.4659871serine/threonine kinase 12
66406−1.4641179hypothetical protein DKFZp762E1312
470124−1.4635711RAD1 (S. pombe) homolog
|
Example IX
[0114] Genes for Discriminating Between IDC Grade I and Grade III
[0115] As shown in Table 8 below, 300 genes were identified as being able to discriminate between two grades of IDC.
8TABLE 8
|
|
CloneIDWeightDescription
|
|
17066353.279305bone gamma-carboxyglutamate (gla) protein (osteocalcin)
6661383.1366533hypothetical protein DKFZp761J1523
7953823.0005724Rap1 guanine-nucleotide-exchange factor directly activated by
cAMP
3648652.8512705hypothetical protein FLJ21062
15005422.8021264regulator of G-protein signalling 11
2701272.7455118
5882622.7127609Homo sapiens, Similar to RIKEN cDNA 2600001A11 gene,
clone MGC: 9907 IMAGE: 3870073, mRNA, complete cds
2772662.5004627Homo sapiens, clone IMAGE: 3625550, mRNA, partial cds
20901292.4882736chromobox homolog 2 (Drosophila Pc class)
3255832.4558352EST
3581512.4309122zinc finger protein 33a (KOX 31)
7866752.3877233epididymis-specific, whey-acidic protein type, four-disulfide
core; putative ovarian carcinoma marker
8547632.378956Homo sapiens cDNA FLJ11341 fis, clone PLACE1010786
15929762.3417116microphthalmia-associated transcription factor
15622312.286418SET binding protein 1
7671762.2472282tumor necrosis factor (ligand) superfamily, member 13
7841782.2026739Homo sapiens mRNA; cDNA DKFZp586M0723 (from clone
DKFZp586M0723)
8111622.1926501fibromodulin
5025182.1886432laminin, beta 2 (laminin S)
7544292.1363368ESTs, Weakly similar to T00084 hypothetical protein
KIAA0512 [H. sapiens]
2142052.1127961Homo sapiens, clone MGC: 17687 IMAGE: 3865868, mRNA,
complete cds
610612.1046094hypothetical protein FLJ20585
3449592.0756965gene for serine/threonine protein kinase
8148152.0694714plakophilin 4
2628042.0673444hypothetical protein MGC2941
14555662.0489061adenosine A3 receptor
14691491.9778151Homo sapiens clone 24606 mRNA sequence
3582171.9703763glypican 4
2062171.9595484nuclear receptor subfamily 1, group H, member 3
2616091.958083ESTs, Weakly similar to S65657 alpha-1C-adrenergic receptor
splice form 2 [H. sapiens]
1975251.9550731flavin containing monooxygenase 5
8576401.9532398collagen, type VI, alpha 2
8121431.9469226fibronectin leucine rich transmembrane protein 3
8982221.9409805Homo sapiens clone 24418 mRNA sequence
4181291.9385868nuclear mitotic apparatus protein 1
20218821.9213983sodium channel, nonvoltage-gated 1, delta
2938191.919948oxidoreductase UCPA
2030031.8989675non-metastatic cells 4, protein expressed in
7052741.8986548diacylglycerol kinase, delta (130 kD)
1249221.8975916KRAB-zinc finger protein SZF1-1
25053101.8859615calcium/calmodulin-dependent protein kinase I
14922381.8795818HSPC003 protein
8103581.8732889acyl-Coenzyme A dehydrogenase, very long chain
23914941.8698169ephrin-A4
15925301.8669954mammalian inositol hexakisphosphate kinase 2
8106711.8659671hypothetical protein FLJ22269
21080481.8648249DNB5
7300361.8564918Mad4 homolog
8107411.831265GABA(A) receptor-associated protein
455781.8274273mitogen-activated protein kinase kinase 6
6473971.8263711ESTs
7674951.8149096GLI-Kruppel family member GLI3 (Greig
cephalopolysyndactyly syndrome)
320501.8117146Homo sapiens mRNA; cDNA DKFZp586P1124 (from clone
DKFZp586P1124)
8118481.8106436hypothetical protein
1863011.8021505Homo sapiens cDNA FLJ12924 fis, clone NT2RP2004709
3665261.7836259ESTs, Weakly similar to ALU5_HUMAN ALU SUBFAMILY SC
SEQUENCE CONTAMINATION WARNING ENTRY
[H. sapiens]
8131541.7815662nuclear factor I/A
851951.778695growth arrest and DNA-damage-inducible, gamma
24601591.7730834tyrosine kinase, non-receptor, 1
5049591.7723846Homo sapiens mRNA; cDNA DKFZp586G0321 (from clone
DKFZp586G0321)
7420941.7684813hypothetical protein FLJ20950
2566191.7658619hydroxysteroid (17-beta) dehydrogenase 7
7266991.7594888ESTs, Weakly similar to AAB47496 NG5 [H. sapiens]
20171441.7514699CGI-41 protein
740701.7336683endosulfine alpha
17621111.7236006natriuretic peptide receptor C/guanylate cyclase C
(atrionatriuretic peptide receptor C)
7957501.7116475Homo sapiens clone 25056 mRNA sequence
20197501.7095011SEC14 (S. cerevisiae)-like 2
23258041.703990395 kDa retinoblastoma protein binding protein
2797201.693594ESTs, Moderately similar to A47582 B-cell growth factor
precursor [H. sapiens]
1328571.6875688Homo sapiens mRNA; cDNA DKFZp586N1323 (from clone
DKFZp586N1323)
18986191.684954hypothetical protein MGC15737
6819921.6802347Homo sapiens cDNA FLJ13384 fis, clone PLACE1001062,
highly similar to Homo sapiens mRNA for lysine-ketoglutarate
reductase/saccharopine dehydrogenase
854501.6753438acyl-Coenzyme A oxidase 2, branched chain
512181.6748931ESTs
19252801.6563658homologous to yeast nitrogen permease (candidate tumor
suppressor)
2508831.6504115ubiquitin-activating enzyme E1-like
1727831.6502064hypothetical protein FLJ10390
2083871.6498608KIAA1407 protein
7039641.6452497inositol polyphosphate phosphatase-like 1
2982311.6428171gamma-aminobutyric acid (GABA) B receptor, 1
2778481.6407233ADP-ribosylation factor 6
6693591.6386236Homo sapiens clone 24405 mRNA sequence
3214551.6327309Homo sapiens, Similar to RIKEN cDNA 1110002C08 gene,
clone MGC: 9564 IMAGE: 3872267, mRNA, complete cds
7961521.6295159Homo sapiens cDNA FLJ11685 fis, clone HEMBA1004934
5027821.614818RAN binding protein 3
3607781.6091782
9505741.606032H3 histone, family 3B (H3.3B)
7883341.5816572mitochondrial ribosomal protein L23
7824971.5767626Homo sapiens, clone IMAGE: 3010666, mRNA, partial cds
17332621.5656438BLu protein
3421811.5642436B-cell CLL/lymphoma 2
16322481.5590794Homo sapiens cDNA FLJ14181 fis, clone NT2RP2004300
15268261.5576997homeo box B2
1451321.5520317mannose-P-dolichol utilization defect 1
1830621.5400324ubiquitin specific protease 21
8236631.5337257fragile X mental retardation, autosomal homolog 2
1900591.5258594guanine nucleotide binding protein (G protein), gamma 7
1117211.5045285insulin induced protein 2
8408821.5039692nucleotide binding protein
19027641.50370166-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
4149991.5021506ets variant gene 4 (E1A enhancer-binding protein, E1AF)
16365231.4948923glutathione S-transferase subunit 13 homolog
5946831.4862053ESTs
19165751.4825298BCL2-interacting killer (apoptosis-inducing)
14569371.4730662oviductal glycoprotein 1, 120 kD (mucin 9, oviductin)
252741.464238g20 protein
7952881.4625618ubiquitin specific protease 4 (proto-oncogene)
15694181.4599089Homo sapiens cDNA FLJ11385 fis, clone HEMBA1000520
7537001.4522717Ras-related GTP-binding protein
16912371.4516493ESTs
7418911.450795RAB2, member RAS oncogene family-like
14933831.4468163Homo sapiens mRNA; cDNA DKFZp434H2418 (from clone
DKFZp434H2418)
16752731.445145RAR-related orphan receptor C
2939161.4265341FKBP-associated protein
396001.4224626adenylate kinase 5
15730871.4200341KIAA0592 protein
8397961.4114662candidate tumor suppressor p33 ING1 homolog
2961231.4057088Homo sapiens PRO1851 mRNA, complete cds
7272631.4046408heterogeneous nuclear ribonucleoprotein U (scaffold
attachment factor A)
2650451.4041143Homo sapiens mRNA; cDNA DKFZp586B0918 (from clone
DKFZp586B0918)
8694501.4034983ribosomal protein L11
7144721.4006155KIAA0397 gene product
8252961.3963737low density lipoprotein receptor defect C complementing
7533011.3962897carcinoembryonic antigen-related cell adhesion molecule 1
(biliary glycoprotein)
7005271.394937glutaredoxin (thioltransferase)
8115651.3909974KIAA1694 protein
16309901.3843882ribosomal protein L29
707491.3810753Homo sapiens cDNA: FLJ21874 fis, clone HEP02488
8148261.3785178ESTs
1751031.376581cadherin, EGF LAG seven-pass G-type receptor 2, flamingo
(Drosophila) homolog
2150001.3677098vasoactive intestinal peptide receptor 1
8103311.3656069quiescin Q6
16814211.3645145EGF-like-domain, multiple 3
5021981.3619124protein phosphatase 1, regulatory (inhibitor) subunit 5
8253651.361275hypothetical protein FLJ21919
15570471.3582677thrombospondin 3
20675001.3579293Z-band alternatively spliced PDZ-motif
4906151.3566637tubulin, gamma 2
7438801.3528093KIAA0263 gene product
8120991.3444147RNA binding motif protein 5
8686521.3432331complement component 4B
1201381.3384804J domain containing protein 1
1563631.3358314hypothetical protein FLJ12934
8135841.3327194p53 regulated PA26 nuclear protein
7267031.3247883Homo sapiens clone 23736 mRNA sequence
8095071.3225829hypothetical protein FLJ20568
20184231.3212314death-associated protein kinase 2
2928061.3201283chromosome segregation 1 (yeast homolog)-like
7419771.3200635B-factor, properdin
610326−4.5776738
322494−3.6341126core-binding factor, beta subunit
824962−3.6328144karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
825659−3.6247426N-myc downstream regulated
796694−3.5038212baculoviral IAP repeat-containing 5 (survivin)
149355−3.4959791translocating chain-associating membrane protein
814270−3.3246381polymyositis/scleroderma autoantigen 1 (75 kD)
884425−3.3113482chaperonin containing TCP1, subunit 5 (epsilon)
1874367−3.2390912small inducible cytokine subfamily A (Cys-Cys), member 20
742707−3.2093502ESTs, Weakly similar to MUC2_HUMAN MUCIN 2
PRECURSOR [H. sapiens]
321354−3.1386611mitochondrial ribosomal protein L15
280375−3.110486PRO2000 protein
2018154−3.0906181electron-transfer-flavoprotein, beta polypeptide
814792−3.0561472ubiquitin specific protease 10
826070−3.0517117KIAA0948 protein
624867−2.9688171hypothetical protein FLJ20186
202035−2.9361687uncharacterized hypothalamus protein HT010
1476053−2.9129535RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
194318−2.8913426hypothetical protein MGC5585
769921−2.8415969ubiquitin carrier protein E2-C
1732922−2.8216808Homo sapiens mRNA; cDNA DKFZp762H106 (from clone
DKFZp762H106)
1536236−2.7995652hypothetical protein FLJ13154
200402−2.7793137hypothetical protein dJ616B8.3
815556−2.7693hypothetical protein FLJ10430
1055607−2.7538365SUMO-1 activating enzyme subunit 1
1466621−2.7448687ATPase, Ca++ transporting, type 2C, member 1
770675−2.6929924Homo sapiens cDNA: FLJ21323 fis, clone COL02374
79520−2.6909833RAB2, member RAS oncogene family
950355−2.6845343ESTs, Weakly similar to S13495 pregnancy zone protein
[H. sapiens]
79710−2.6813265KIAA0174 gene product
2011138−2.6569485KIAA1036 protein
898333−2.6509707ESTs
878846−2.6361347brain protein 13
842818−2.63282lysyl-tRNA synthetase
1553065−2.627227hypothetical protein FLJ14993
136722−2.6269431ATPase, Na+/K+ transporting, beta 3 polypeptide
308633−2.6261051hypothetical protein FLJ10339
854581−2.6159344transcription factor 4
951241−2.5987959clone HQ0310 PRO0310p1
292936−2.5940369hypothetical protein FLJ10468
781047−2.569753budding uninhibited by benzimidazoles 1 (yeast homolog)
823907−2.5587562hypothetical protein FLJ10511
377368−2.5495557cell death regulator aven
292388−2.5448438
122241−2.539248proteasome (prosome, macropain) subunit, beta type, 2
731223−2.5364769proteasome (prosome, macropain) subunit, beta type, 2
1660666−2.534682carbonic anhydrase VB, mitochondrial
825470−2.530053topoisomerase (DNA) II alpha (170 kD)
1472719−2.526978SMT3 (suppressor of mif two 3, yeast) homolog 1
869375−2.5249164isocitrate dehydrogenase 2 (NADP+), mitochondrial
302292−2.5097213exostoses (multiple) 2
1175007−2.4998247tumor necrosis factor receptor superfamily, member 10d,
decoy with truncated death domain
1422338−2.488461ribonucleotide reductase M2 polypeptide
2014034−2.4843808methylene tetrahydrofolate dehydrogenase (NAD+
dependent), methenyltetrahydrofolate cyclohydrolase
50884−2.4377483dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
1474424−2.4345879Homo sapiens cDNA FLJ12758 fis, clone NT2RP2001328
753285−2.4324065glycogenin
700792−2.4311609cyclin-dependent kinase inhibitor 3 (CDK2-associated dual
specificity phosphatase)
259950−2.4310983hypothetical protein FLJ14991
610326−2.4214624
284004−2.4185183ESTs
756595−2.3963227S100 calcium-binding protein A10 (annexin II ligand, calpactin
I, light polypeptide (p11))
128711−2.3891503anillin (Drosophila Scraps homolog), actin binding protein
1582738−2.3882015uncharacterized bone marrow protein BM040
1915867−2.3829702Homo sapiens cDNA: FLJ21086 fis, clone CAS03272
754582−2.3765311ecotropic viral integration site 2A
462961−2.3685047dihydrofolate reductase
705064−2.3682586transforming, acidic coiled-coil containing protein 3
1553696−2.3605995vanin 1
129294−2.3507956ESTs
809588−2.3342142gamma-glutamyl hydrolase (conjugase,
folylpolygammaglutamyl hydrolase)
1909526−2.333814ESTs, Weakly similar to 2004399A chromosomal protein
[H. sapiens]
815771−2.3166739chromosome 7 open reading frame 2
703707−2.2865473aspartate beta-hydroxylase
2017415−2.2832169centromere protein A (17 kD)
1650942−2.2797717Human proteinase activated receptor-2 mRNA, 3′UTR
645079−2.27619463′(2′), 5′-bisphosphate nucleotidase 1
133213−2.2753496fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-
specific)
788566−2.2752004Purkinje cell protein 4
2009574−2.2646007T brachyury (mouse) homolog
1897302−2.2563379serine/threonine kinase 17b (apoptosis-inducing)
78869−2.2539078cell membrane glycoprotein, 110000M(r) (surface antigen)
590759−2.2513499sterol-C4-methyl oxidase-like
785368−2.2489394PDZ-binding kinase; T-cell originated protein kinase
1714689−2.2489064ESTs
795197−2.2481741ubiquitin-conjugating enzyme E2E 2 (homologous to yeast
UBC4/5)
781472−2.2390098Taxi (human T-cell leukemia virus type I) binding protein 1
1469966−2.2375228delta (Drosophila)-like 3
1587847−2.2354975minichromosome maintenance deficient (mis5, S. pombe) 6
209066−2.2325695
1035796−2.2308926ESTs
233464−2.2280935epithelial V-like antigen 1
753320−2.2104141hypothetical protein FLJ20533
486626−2.2095389ESTs
1505038−2.2060272hypothetical protein FLJ20171
503851−2.1992378nuclear receptor co-repressor/HDAC3 complex subunit
503671−2.1939286Homo sapiens cDNA FLJ14368 fis, clone HEMBA1001122
610326−2.1885269
712139−2.1839319ADP-ribosylation factor-like 7
471196−2.1797647integral membrane protein 3
753215−2.1781545guanine nucleotide binding protein (G protein), alpha inhibiting
activity polypeptide 1
183556−2.1734918gap junction protein, alpha 4, 37 kD (connexin 37)
809557−2.1667294minichromosome maintenance deficient (S. cerevisiae) 3
610326−2.1633594hypothetical protein MGC12992
1600239−2.1602202HSPC037 protein
1049291−2.1593791olfactory receptor, family 7, subfamily E, member 47
pseudogene
839682−2.1475459ubiquitin-conjugating enzyme E2N (homologous to yeast
UBC13)
950897−2.1419057Homo sapiens mRNA; cDNA DKFZp586G1922 (from clone
DKFZp586G1922)
712577−2.1411578holocytochrome c synthase (cytochrome c heme-lyase)
824524−2.1400285UDP-galactose transporter related
241348−2.1301572prenylcysteine lyase
823930−2.1259223actin related protein 2/3 complex, subunit 1A (41 kD)
150314−2.1206632lysophospholipase I
795498−2.1190094putative transmembrane protein
82710−2.1184513glutathione S-transferase A2
1869201−2.1135906hypothetical protein MGC2745
1694526−2.1126121hypothetical protein FLJ11029
823598−2.1072985proteasome (prosome, macropain) 26S subunit, non-ATPase,
12
214162−2.1065234metallothionein 1H
811590−2.1050783hypothetical protein FLJ11100
796904−2.1050163pleiomorphic adenoma gene-like 1
1517595−2.0965219KIAA0175 gene product
282428−2.0954489ESTs, Weakly similar to A46010 X-linked retinopathy protein
[H. sapiens]
1883219−2.0949091ESTs
746229−2.0906355mitogen-activated protein kinase kinase kinase kinase 4
610326−2.0896786hypothetical protein MGC12992
1632225−2.0828168ESTs
743810−2.0796998hypothetical protein MGC2577
1416055−2.0725881extra spindle poles, S. cerevisiae, homolog of
1626297−2.0656173hypothetical protein FLJ20509
852829−2.0608067karyopherin alpha 3 (importin alpha 4)
840511−2.0554449vimentin
278504−2.0519206neurochondrin
1534700−2.0506628KIAA0830 protein
812276−2.0458249synuclein, alpha (non A4 component of amyloid precursor)
306841−2.0449642T cell receptor beta locus
3172883−2.0437672ESTs, Weakly similar to 1709359A dopamine D4 receptor
[H. sapiens]
624627−2.0409259ribonucleotide reductase M2 polypeptide
486179−2.0380168Homo sapiens cDNA FLJ10205 fis, clone HEMBA1004954
376316−2.0218598wee1+ (S. pombe) homolog
37671−2.0190035hypothetical protein FLJ21610
629944−2.0166696myosin VB
713158−2.0133834ESTs
504308−2.0067977hypothetical protein FLJ10540
1605426−1.9957389hypothetical protein FLJ13352
810711−1.9920785stearoyl-CoA desaturase (delta-9-desaturase)
263790−1.9904129isocitrate dehydrogenase 3 (NAD+) alpha
549349−1.9893582hypothetical protein PRO1068
109221−1.9879196KIAA0286 protein
51773−1.9875155hypothetical protein MGC3077
|
Example X
[0116] Crossvalidation
[0117] The following table shows the results of crossvalidation analysis (as described in Example I above) of various pair-wise comparisons. The two members of the pairwise comparison are indicated as “Class 1” and “Class 2” with the number of samples analyzed indicated under “N”. The number of genes used to predict is indicated, along with the accuracy (in percent) for each combination. “FP” and “FN” refer to “false positive” and “false negative”, respectively, as incorrectly identified for each of the two classes.
9|
|
Class 1Class 2Accu-Class 1Class 2
NameNNameNGenesracyFPFNFPFN
|
Normal28Abnormal57850990110
Normal28ADH76001000000
Normal28DCIS2813001000000
ADH7DCIS28350970110
ADH7DCIS2810970110
N + ADH36DCIS + IDC49400981111
DCIS-I7DCIS-III93001000000
IDC-I4IDC-III83001000000
|
[0118] References:
[0119] DeRisi, J., et al., Use of a cDNA microarray to analyse gene expression patterns in human cancer, Nature Genetics, (1996) 14:457-460.
[0120] Hedenfalk, I., et al., Gene-Expression Profiles In Heredity Breast Cancer, The New England Journal of Medicine, (Feb. 22, 2001) 344:8:539-548.
[0121] Golub, T. R., et al., Molecular Classification of Cancer: Class Discovery and Class Prediction by Gene Expression Monitoring, Science, (Oct. 15, 1999) 286:531-537.
[0122] Perou, Charles M., et al., Molecular portraits of human breast tumours, Nature, (Aug. 17, 2000) 406:747-752.
[0123] Garber, Mitchell E., et al., Diversity of gene expression in adenocarcinoma of the lung, Proc. Natl. Acad. Sci. USA, (Nov. 20, 2001) 98:24:13784-13789.
[0124] Perou, Charles M., et al., Distinctive gene expression patterns in human mammary epithelial cells and breast cancers, Proc. Natl. Acad. Sci. USA, (Aug. 1999) 96:9212-9217.
[0125] Sgrio, Dennis C., et al., In Vivo Gene Expression Profile Analysis of human Breast Cancer Progression, Cancer Research, (Nov. 15, 1999) 59:5656-5661.
[0126] Sorlie, Therese, et al., Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications, Proc. Natl. Acad. Sci., (Sep. 11, 2001) 98:19:10869-10874.
[0127] Alizadeh, Ash A., et al., Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling, Nature, (Feb. 3, 2000) 403:503-511.
[0128] Bittner, M., et al., Molecular classification of cutaneous malignant melanoma by gene expression profiling, Nature (Aug. 3, 2000) 406:536-540.
[0129] West, Mike, et al., Predicting the clinical status of human breast cancer by using gene expression profiles, Proc. Natl. Acad. Sci., (Sep. 25, 2001) 98:20:11462-11467.
[0130] All references cited herein, including patents, patent applications, and publications, are hereby incorporated by reference in their entireties, whether previously specifically incorporated or not.
[0131] Having now fully described this invention, it will be appreciated by those skilled in the art that the same can be performed within a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While this invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications. This application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains and as may be applied to the essential features hereinbefore set forth.
Claims
- 1. An array comprising more than one of the genes in any one of Tables 2-5 hybridized to nucleic acids derived from a cell suspected of being abnormal or malignant.
- 2. The array of claim 1 wherein more than one comprises more than 5 of the genes in any one of Tables 2-5.
- 3. The array of claim 1 wherein more than one comprises more than 10 of the genes in any one of Tables 2-5.
- 4. The array of claim 1 wherein more than one comprises more than 11 of the genes in any one of Tables 2-5.
- 5. The array of claim 1 wherein said cell is from a subject afflicted with, or suspected of having, breast cancer.
- 6. The array of claim 5 wherein said subject is human.
- 7. An array comprising more than one of the genes in Tables 1 or 6 hybridized to nucleic acids derived from a cell suspected of being hyperplastic or cancerous.
- 8. The array of claim 7 wherein more than one comprises more than 5 of the genes in Tables 1 or 6.
- 9. The array of claim 7 wherein more than one comprises more than 10 of the genes in Tables 1 or 6.
- 10. The array of claim 7 wherein more than one comprises more than 11 of the genes in Tables 1 or 6.
- 11. The array of claim 7 wherein said cell is from a subject afflicted with, or suspected of having, breast cancer.
- 12. The array of claim 11 wherein said subject is human.
- 13. An array comprising more than one of the genes in Table 7 hybridized to nucleic acids derived from a DCIS cell.
- 14. An array comprising more than one of the genes in Table 8 hybridized to nucleic acids derived from a IDC cell.
- 15. A method to determine the breast cancer stage of a ductal lavage or fine needle aspiration sample from a subject comprising assaying said sample for expression of one or more genes correlated with one or more stages of breast cancer.
- 14. The method of claim 15 wherein said assaying comprises preparing RNA from said sample.
- 15. The method of claim 14 wherein said RNA is amplified.
- 16. The met hod of claim 15 wherein said assaying comprises using an array.
- 17. The method of claim 15 wherein said assaying comprises using the array of claim 1.
- 18. The method of claim 15 wherein said one or more genes are correlated with ADH, DCIS, and/or IDC.
- 19. The method of claim 15 wherein said one or more genese are correlated with normal and abnormal cells.
- 20. A method to determine breast cancer stage of a cell containing sample from a subject comprising assaying said sample for expression of one or more genes capable of discriminating between two stages of breast cancer.
- 21. The method of claim 20 wherein said sample is from a subject afflicted with, or suspected of having, breast cancer.
- 22. The method of claim 20 wherein said subject is human.
- 23. The method of claim 20 wherein said sample is a microdissected sample.
- 24. The method of claim 23 wherein said sample is microdissected via laser capture microdissection.
- 25. The method of claim 20 wherein said one or more genes discriminate between normal and abnormal cells, between cancerous and non-cancerous cells, or between stages of DCIS or IDC.
- 26. A method to determine therapeutic treatment for a patient determined to have atypical cells in a sample therefrom comprising
assaying said cells for expression of genes correlated with non-cancerous and cancerous breast cancer cells, identifying the stage of breast cancer of said cells, and selecting the appropriate treatment for a patient having cells of such a stage.
- 27. A method to identify genes the expression of which are correlated with one or more stages of breast cancer comprising
obtaining multiple homogenous populations of breast cancer cells from each of said one or more stages; identifying more than one gene the expression of which is correlated with said one or more stage by detecting genes with similar expression profiles in more than one of said populations.