Claims
- 1. A canola protein isolate having a protein content of at least about 90 wt % on a dry weight basis and at a Kjeldahl nitrogen conversion of N×6.25 and which exhibits a protein profile which is:
about 60 to about 95% wt % of 2S protein, about 5 to about 40 wt % of 7S protein, and 0 to about 5 wt % of 12S protein.
- 2. The isolate of claim 1 which exhibits a protein profile which is:
about 70 to about 95 wt % of 2S protein, about 5 to about 30 wt % of 7S protein, and 0 to about 2 wt % of 12S protein.
- 3. The isolate of claim 2 having a protein content of at least about 100 wt %.
- 4. A canola protein isolate composition comprising:
(1) a first canola protein isolate having a protein content of at least about 90 wt % on a dry weight basis and at a Kjeldahl nitrogen conversion of N×6.25 and which exhibits a protein profile which is:
about 60 to about 98 wt % of 7S protein, about 1 to about 15 wt % of 12S protein, 0 to about 25 wt % of 2S protein, and (2) a second canola protein isolate having a protein content of at least about 90 wt % on a dry weight basis and a Kjeldahl nitrogen conversion of N×6.25 and which exhibits a protein profile which is:
about 60 to about 95 wt % of 2S protein about 5 to about 40 wt % of 7S protein 0 to about 5 wt % of 12S protein.
- 5. The composition of claim 4 wherein said first canola protein isolate and said second canola protein isolate are present in said composition in a weight ratio of about 5:95 to about 95:5.
- 6. The composition of claim 5 wherein said first canola protein isolate exhibits a protein profile which is:
about 88 to about 98 wt % of 7S protein, about 1 to about 10 wt % of 12S protein, and 0 to about 6 wt % of 2S protein.
- 7. The composition of claim 6 wherein said first canola protein isolate has a protein content of at least about 100 wt %.
- 8. The composition of claim 5 wherein the second canola protein isolate exhibits a protein profile which is:
about 70 to about 95 wt % of 2S protein, about 5 to about 30 wt % of 7S protein, and 0 to about 2 wt % of 12S protein.
- 9. The composition of claim 8 wherein the second canola protein isolate has a protein content of at least about 100 wt %.
- 10. An isolated and purified 7S protein of canola.
- 11. The protein of claim 10 having a molecular weight, as determined by MALDI-MS, of about 145 kDa.
- 12. The protein of claim 11 which is comprised of three subunits, each sized approximately 413 amino acids.
- 13. The protein of claim 12 having an amino acid analysis substantially as set forth in Table VII or Table VIII.
- 14. The protein of claim 10 which is isolated and purified from a canola protein micellar mass.
- 15. The protein of claim 14 wherein said protein micellar mass is formed by diluting a concentrated saline solution of canola protein extracted from canola oil seed meal under mildly acidic conditions.
- 16. A composition, comprising a mixture of an isolated and purified 7S protein of canola protein and at least one additional isolated and purified canola protein extracted from the group consisting of the 2S and 12S canola proteins.
- 17. A method of isolating and purifying an individual canola protein from a canola protein isolate containing a mixture of at least two different canola proteins selected from the group consisting of the 2S, 7S and 12S proteins, which comprises:
providing said canola protein isolate having a protein contents of at least about 90 wt % on a dry weight basis and at a Kjeldahl nitrogen conversion of N×6.25, solubilizing the canola protein isolate, subjecting the resulting protein solution to preparative high pressure liquid chromatography or equivalent procedure separating proteins on the basis of molecular size, collecting fractions of eluant containing the individual selected canola protein, subjecting the selected fractions to ultrafiltration to reduce the volume of eluant, dialyzing the concentrated eluant to remove solubilizing material, and drying the dialyzate to recover the individual canola protein.
- 18. The method of claim 17 wherein said canola protein isolate is one which exhibits a protein profile which is:
about 60 to about 98 wt % of 7S protein, about 1 to about 15 wt % of 12S protein, and 0 to about 25 wt % of 2S protein.
- 19. The method of claim 18 wherein said canola protein exhibits a protein profile which is:
about 88 to about 98 wt % of 7S protein, about 1 to about 10 wt % of 12S protein, and 0 to about 6 wt % of 2S protein.
- 20. The method of claim 18 wherein said canola protein isolate is processed to isolate and purify the 7S and/or 12S proteins.
- 21. The method of claim 17 wherein said canola protein isolate is one which exhibits a protein profile which is:
about 60 to about 95 wt % of 2S protein, about 5 to about 30 wt % of 7S protein, 0 to about 2 wt % of 12S protein.
- 22. The method of claim 21 wherein said canola protein isolate exhibits a protein profile which is:
about 70 to about 95 wt % of 2S protein, about 5 to about 30 wt % of 7S proteins, 0 to about 2 wt % of 12S protein.
- 23. The method of claim 21 wherein said canola protein isolate is processed to isolate and purify the 2S protein.
REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority pursuant to 35 USC 119(e) from copending U.S. Patent Applications Nos. 60/372,165 filed Apr. 15, 2002 and 60/430,687 filed Dec. 4, 2002.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60372165 |
Apr 2002 |
US |
|
60430687 |
Dec 2002 |
US |