Patent Application
20120111727
The present invention relates to size separation of proteins by capillary electrophoresis in sieving media, wherein one or more cationic surfactants form charged complexes with the proteins and equalize their surface charge density, making their migration in sieving media independent of their intrinsic charge and thus allowing their size separation and molecular-weight determination. Specifically, the invention is directed to capillary sieving electrophoresis of proteins in the presence of cationic surfactants at low pH.
Electrophoretic sieving media are used to size separate biopolymers: nucleic acids, polysaccharides, and proteins. They provide a system of obstacles in the electrophoretic migration path so that migrating biopolymers collide with the obstacles and these collisions retard their apparent migration velocity. Larger molecules and particles are retarded in their migration more than small molecules. The first electrophoretic sieving media were starch and polyacrylamide gel. Nucleic acids are equally ionized at non-acidic pH and need not be modified to size separate during electrophoretic migration in sieving media. On the other hand, protein ionization and charge significantly vary depending on the amino acid composition. Therefore, native proteins are not size separated in sieving media in absence of ionic surfactants. However, when heated with an ionic surfactant, proteins denature and bind the surfactants, generating complexes with more or less equal surface charge density. These complexes migrate in sieving media according to their size. Ionic surfactants such as sodium dodecyl sulfate (SDS) (Shapiro, Vinuela, and Maizel, 1967), cetyltrimethylammonium bromide (CTAB) (Panyim, Thitipongpanich, and Supatimusro, 1977), cetylpyridinium chloride (Schick, 1975) have been used to equalize the surface charge density of proteins prior electrophoresis.
SDS electrophoresis in polyacrylamide slab gel (SDS PAGE) was the first method separating proteins according to their size (Shapiro, Vinuela, and Maizel, 1967; Shapiro and Maizel, 1969; Weber and Osborn, 1969; Dunker and Rueckert, 1969). Formation of SDS-protein complexes is independent of ionic strength (Reynolds and Tanford, 1970). However, some proteins exhibit an anomalous migration in SDS PAGE (Shapiro, Vinuela, and Maizel, 1967; Williams and Gratzer, 1971). The anomalous migration of acidic proteins in SDS PAGE was, however, normalized by esterification of carboxyl groups (Williams and Gratzer, 1971) suggesting insufficient surfactant binding. This hypothesis was corroborated by an observation that some acidic proteins, such as pepsin, papain, and glucose oxidase do not bind measurable amount of SDS (Nelson, 1971).
Shortly after the invention of SDS PAGE, a method separating proteins by polyacrylamide gel electrophoresis (PAGE) in the presence of cationic surfactants was described (Williams and Gratzer, 1971). A study based on observations of behavior of protein-cationic-surfactant-complexes followed, predicting a failure of the electrophoresis in the presence of cationic surfactants to determine molecular weights of proteins (Nozaki, Reynolds, and Tanford, 1974). Later, cetylpyridinium chloride (Schick, 1975) and cetyltrimethylammonium bromide (Akins, Levin, and Tuan, 1992; Akins and Tuan, 1994; Akin, Shapira, and Kinkade Jr., 1985; Eley, Burns, Kannapell, and Campbell, 1979; Panyim, Thitipongpanich, and Supatimusro, 1977) were used for size separations of proteins by PAGE. Several protocols have been developed to denature proteins with cetyltrimethylammonium bromide (Akins, Levin, and Tuan, 1992; Akins and Tuan, 1994; Akin, Shapira, and Kinkade Jr., 1985; Eley, Burns, Kannapell, and Campbell, 1979; Panyim, Thitipongpanich, and Supatimusro, 1977), including a protocol without any heating of the sample (Akins, Levin, and Tuan, 1992).
When electrophoresis of proteins in sieving media was transferred from slab gels into capillaries, crosslinked polyacrylamide gel was initially used as the sieving matrix (Hjerten, 1983; Cohen and Karger, 1987). When linear hydrophilic polymers were introduced as a replaceable sieving matrix for separation of polynucleotides (Hjerten, Valtcheva, Elenbring, and Eaker, 1989), various polymers were introduced for electrophoretic size separation of biopolymers, including linear polyacrylamide (Ganzler, Greve, Cohen, Karger, Guttman, and Cooke, 1992a; Sudor, Foret, and Bocek, 1991; Heiger, Cohen, and Karger, 1990), poly(ethylene oxide) (Guttman, Nolan, and Cooke, 1993), dextran (Ganzler, Greve, Cohen, Karger, Guttman, and Cooke, 1992b), guaran (Dolnik, Gurske, and Padua, 2001a), glucomannan (Izumi, Yamaguchi, Yoneda, Isobe, Okuyama, and Shinoda, 1993), poly(vinyl alcohol) (Kleemiss, Gilges, and Schomburg, 1993), poly(dimethyl acrylamide) (Madabhushi, 1998), poly(hydroxypropyl acrylamide) (Lindberg, Righetti, Gelfi, and Roeraade, 1997), poly(ethoxyethyl acrylamide) (Chiari, Nesi, and Righetti, 1994; Chiari, Riva, Gelain, Vitale, and Turati, 1997), agarose (Motsch, Kleemiss, and Schomburg, 1991), and pullulan (Nakatani, Shibukawa, and Nakagawa, 1994; Nakatani, Shibukawa, and Nakagawa, 1996). Capillary size separations of proteins were performed exclusively by SDS capillary sieving electrophoresis (CSE). The method was also modified for size separation of proteins on microchip (Yao, Anex, Caldwell, Arnold, Smith, and Schultz, 1999) with poly(dimethyl acrylamide) as a sieving polymer (Yao, Anex, Caldwell, Arnold, Smith, and Schultz, 1999). (Bousse, Mouradian, Minalla, Yee, Williams, and Dubrow, 2001) Capillary electrophoresis brought a number of advantages as compared to electrophoresis in slab gel: faster analysis, automation, higher separation efficiency, and higher detection sensitivity. Nevertheless, a small size of capillaries emphasized the effect of the capillary wall: typically the used fused silica capillaries contained ionized silanol groups on their internal surface, resulting in strong wall adsorption, significant electroosmotic flow, eddy migration, and consequent mediocre separation efficiency. Electroosmotic flow was eventually suppressed by applying a hydrolytically stable neutral coating on the capillary wall (U.S. Pat. No. 5,143,753). Nevertheless, in SDS CSE, SDS may adsorb on the neutral coating and generate secondary electroosmotic flow resulting in mediocre reproducibility and separation efficiency.
In SDS CSE, electroosmotic flow was also suppressed by applying high-concentration Tris-borate buffer (U.S. Patent application 20040050702). Borate complexes with polyol bases were also used to suppress electroosmotic flow in other CE applications (U.S. patent application Ser. No. 13/342,031).
Hypothetically, electroosmotic flow in SDS CSE could be also suppressed by reducing pH of the sieving medium and a consequent suppression of the silanol ionization in the capillary wall. However, SDS binding of proteins is weaker at pH<6 and SDS electrophoresis at this pH results in significantly broader peaks (Gilbert, 1995) excluding this alternative from a real world practice.
When migration of biopolymers in sieving media was studied in a greater detail, three distinct migration modes were identified, depending on the pore size of the sieving matrix and size of the polyelectrolyte: Ogston mode, reptation without stretching, and reptation with stretching (Noolandi J., 1992). In capillary electrophoresis, where sieving polymers serve as the sieving matrix, the pore size of the sieving matrix is fine tuned by changing the concentration of the sieving polymer (Ganzler, Greve, Cohen, Karger, Guttman, and Cooke, 1992a; Karim, Janson, and Takagi, 1994). Molecular weight of the sieving polymer also plays an important role, particularly in separation of large polyelectrolytes migrating in the reptation-with-stretching mode (Bae and Soane, 1993). High-molecular-weight polymers are typically better sieving matrices than those with low molecular weight (Dolnik, Gurske, and Padua, 2001b) although blends of polymers of high and low molecular weight were also advocated and used (Salas-Solano, RuizMartinez, Carrilho, Kotler, and Karger, 1998). Stiffness and branching of the sieving polymers affects the sieving properties of the sieving matrix as well: branched polysaccharides such as dextran or Ficoll® exhibit rather limited sieving and are suitable more for separation of polyelectrolytes migrating in the Ogston mode. Stiff polysaccharides such as hydroxyethyl cellulose, guaran, and locust bean gum (Dolnik, Gurske, and Padua, 2001a) form a rigid sieving matrix at lower molecular weights and/or concentrations than easily bending synthetic polymers such as linear polyacrylamide. In practical CSE analysis, viscosity is a limiting factor since pressure as high as 900 psi is necessary to replace a viscous sieving matrix in the capillary. The viscosity of the sieving matrix makes a practical limit when increasing the concentration and molecular weight of the sieving polymer. In DNA sequencing by capillary electrophoresis, the weight molecular weight (Mw, further molecular weight) of linear polyacrylamide used as a sieving polymer, exceeded 10,000,000 (Goetzinger, Kotler, Carrilho, RuizMartinez, SalasSolano, and Karger, 1998). In CSE of proteins, most proteins were separated in sieving media with large pore size and sieving polymers typically did not exceed molecular weight of 1 million. To provide efficient sieving, the sieving polymers should have molecular weight about 100,000 and more. However, this value is rather arbitrary, as lower-molecular-weight polymers can be prepared at a higher concentration and still keep the viscosity of the solution at an acceptable level. Stiff polysaccharides, such as hydroxyethyl cellulose, guaran, or locust bean gum can be used even at a lower molecular weight. On the other hand, branched polysaccharides, such as dextran, are preferably used at high molecular weight (2 million and higher).
Cetyltrimethylammonium bromide was used in capillary electrophoresis for two purposes: a) as a dynamic coating to reverse the direction of electroosmotic flow (Reijenga, Aben, Verheggen, and Everaerts, 1983; Tsuda, 1987; Corradini, 1997; Ding and Fritz, 1997; Chiari, Damin, and Reijenga, 1998), b) as a pseudostationary phase in capillary electrokinetic micellar chromatrography (Ong, Ng, Lee, and Li, 1994). None cationic surfactant has been used for size separation of proteins by capillary sieving electrophoresis yet. An interesting group of potential cationic surfactants are gemini surfactants (Menger and Keiper, 2000) with two amino groups connected by an aliphatic arm but they have not been used in capillary electrophoresis yet.
The present invention is suitable for a fast, quantitative, and highly reproducible size separation of proteins by means of capillary sieving electrophoresis. Disclosed herein are a composition of a separation medium and a protein denaturing solution, and a method of capillary sieving electrophoresis in the presence of a cationic surfactant for size separation of proteins with molecular weight in the range between about 14,000 and about 500,000. In the preferred embodiment, the separation medium comprises an acidic buffer that keeps pH in the range between about 3 and about 5, a hydrophilic sieving polymer with a moderate viscosity, and between about 0.5 and about 30 g/L cationic surfactant.
A cationic surfactant used in the sieving matrix should exhibit a sufficient solubility in water and, simultaneously it should strongly bind proteins. Primary, secondary, tertiary or quaternary amines with one or more long aliphatic chains are suitable cationic surfactants for CSE of proteins. Typically longer aliphatic chains are preferred because then the surfactant binds proteins more strongly. However, solubility of the cationic surfactant in water may be a limiting factor for its practical use. Cationic surfactants suitable for capillary sieving electrophoresis of proteins contain one or more of the following cations: octadecyldimethylethylammonium, cetyldimethylethylammonium, tetradecyldimethylethylammonium, dodecyldimethylethylammonium, octadecyltrimethylammonium, cetyltrimethylammonium, tetradecyltrimethylammonium, dodecyltrimethylammonium, octadecylpyridinium, tetradecylpyridinium, dodecylpyridinium, octadecylammonium, cetylammonium, tetradecylammonium, dodecylammonium, decylammonium, didodecyldimethylammonium, and a cationic gemini surfactant alkanediyl-.α.,.ω.-bis(dimethylalkylammonium), with a formula CmH2m+1(CH3)2N+(CH2)sN+(CH3)2CmH2m+1, m being 12, 14, 16, or 18 and s being 2, 3, 4, 5, 6, 7, or 8.
The counter anion in the cationic surfactant can be an inorganic anion such as chloride, bromide, sulfate, bicarbonate, etc. The nature of the counter anion plays a significant role. In capillary sieving electrophoresis of monoclonal antibodies, a broad system peak appears when cetyltrimethylammonium bromide is used as the cationic surfactant. With cetyltrimethylammonium chloride, the system peak is much smaller and such a sieving medium is more preferred.
Size separations of proteins in capillary format based on SDS frequently suffer from mediocre separation efficiency and lower reproducibility of qualitative and quantitative analysis. Capillary sieving electrophoresis of proteins with cationic surfactant at low pH, where ionization of silanol groups in the capillary wall is reduced can perform better. The separation medium contains a cationic surfactant, a sieving polymer, and an acidic buffer with pH below 5.
However, below pH 3, the high-mobility H+ ion contributes significantly to the conductivity of the sieving matrix. This results in elevated Joule heat and overheating of the capillary. Experiments show, keeping the pH of the sieving matrix at about pH 4 is the best solution.
One possibility is to use a free weak acid, e.g., acetic acid, as the only electrolyte. Another option is to use fully ionized cation, e.g., Tris, with a buffering anion, e.g., formate, acetate, propionate, butyrate, capronate, valproate, pimelate, fumarate, maleate, succinate, glutarate, adipate, malate, tartrate, glycolate, lactate, 2-hydroxybutyrate, 2-hydroxyisobutyrate, citrate, nicotinate, glutamate, and aspartate. pH can be also kept at a adequate value with a buffering cation, e.g., β-alanine, γ-aminobutyric acid (GABA), glycine, ε-aminocaproic acid, or nicotinamide and a fully ionize anion. Probably the most attractive buffering option is an equimolar mixture of a weak base and a weak acid, having close pK's, e.g., GABA (pK 4.0) and glutamic acid (pK 4.2), β-alanine (pK 3.6) and glutamic acid (pK 4.2), and β-alanine (pK 3.6) and 2-hydroxy-isobutyric acid (pK 3.9). If dicarboxylic or tricarboxylic acids are used as a buffer, they have to be used at pH, where only one carboxylic group is partly dissociated. Other factors than pK, which may be difficult to predict from the physicochemical properties of the buffers, may be also important: Buffers containing β-alanine show better separation efficiency than buffers with GABA, but in its presence, proteins more strongly adsorb on the wall. This results in a minor but discernable baseline elevation when proteins migrate through the detection cell.
The sieving matrix enables size separation of proteins. It provides obstacles in the migration path and forces proteins-surfactants complexes to electrophoretically migrate according to their size. The sieving polymer should (i) be soluble in water, (ii) be non-ionic, (iii) not significantly bind cationic surfactants, (iv) should have sufficient molecular weight and provide sufficient sieving, (v) not absorb UV light if UV detection is used. The sieving properties of the matrix can be fine tuned by changing the molecular weight and concentration of the sieving polymer. Higher concentration and/or higher molecular weight of the sieving polymer results in smaller pores, i.e., in more efficient sieving but also in longer migration times. Simultaneously, the viscosity rises and may prevent fast replacement of the sieving matrix in the capillary. Linear polysaccharides, such as hydroxyethyl cellulose, hydroxypropyl cellulose, guaran, locust bean gum, glucomannan, and pullulan, have a stiff molecule and will be typically used at concentrations from about 4 to about 60 g/L and molecular weight from about 20,000 to about 500,000. Hydrophilic synthetic polymers, such as linear polyacrylamide, poly(dimethyl acrylamide), poly(hydroxyethyl acrylamide), poly(hydroxypropyl acrylamide), poly(ethoxyethyl acrylamide), poly(vinyl alcohol), poly(vinyl pyrrolidone), and poly(ethylene oxide) will be effective sieving polymers at concentration from about 8 to about 80 g/L and molecular weight from about 100,000 to about 1 million. Branched polysaccharides such as dextran or Ficoll® are less efficient sieving polymers and have to be used at concentration from about 100 to about 400 g/L, at molecular weight of about 2 million. UV absorbing polymers, such as poly(vinyl pyrrolidone) will not properly work in CSE with UV detection but may be used in CSE with laser induced fluorescence detection.
We disclose here a protein denaturing solution for sample preparation prior capillary electrophoretic size separation of proteins, comprising:
Further we disclose here a protein denaturing solution for sample preparation prior capillary electrophoretic size separation of proteins of claim 1, wherein said protein denaturing solution comprises about 10 g/L cetyldimethylethylammonium bromide, about 100 mM potassium phosphate, and about 10 g/L dithiothreitol.
We disclose also a protein denaturing solution for sample preparation of proteins prior capillary electrophoretic size separation, wherein said protein denaturing solution comprises about 10 g/L cetyldimethylethylammonium bromide, about 100 mM potassium chloride, and about 10 g/L dithiothreitol.
Further we disclose here a protein denaturing solution for sample preparation of proteins prior capillary electrophoretic size separation, wherein said protein denaturing solution comprises about 10 g/L cetyltrimethylammonium chloride, about 100 mM potassium phosphate, and about 10 g/L dithiothreitol.
We also disclose here a protein denaturing solution for sample preparation of proteins prior capillary electrophoretic size separation, wherein said protein denaturing solution comprises about 10 g/L cetyltrimethylammonium chloride, about 100 mM potassium phosphate, and from about 10 mM to about 50 mM tris-(carboxyethyl)phosphine.
Further we disclose a protein denaturing solution for sample preparation of proteins prior capillary electrophoretic size separation, wherein said protein denaturing solution comprises about 10 g/L cetyltrimethylammonium bromide, about 100 mM potassium chloride, and from about 20 mM to about 50 mM tris-(carboxyethyl)phosphine.
We disclose here a method of sample preparation prior capillary electrophoretic size separation of proteins, wherein a protein sample is dissolved in the protein denaturation solution and incubated at temperature from about 60 to about 100° C. for a time interval from about 1 min to about 20 min.
Further we disclose here a method of sample preparation prior capillary electrophoretic size separation of proteins, wherein a protein sample is dissolved in the protein denaturation solution and incubated at about 90° C. for about 2 min.
We also disclose here a method of sample preparation prior capillary electrophoretic size separation of proteins, wherein a protein sample is dissolved in the protein denaturation solution and incubated at about 70° C. for about 10 min.
Further we disclose here a separation medium for capillary electrophoretic size separation of proteins, consisting essentially of:
We disclose here a separation medium for capillary electrophoretic size separation of proteins, wherein said cationic surfactant is cetyldimethylethylammonium bromide in the concentration range from about 0.5 g/L to about 30 g/L.
Further we disclose here a separation medium for capillary electrophoretic size separation of proteins, wherein said cationic surfactant is tetradecyltrimethylammonium bromide in the concentration range from about 0.5 g/L to about 30 g/L.
We disclose here a separation medium for capillary electrophoretic size separation of proteins, wherein said acid buffer comprises from about 20 mM to about 200 mM β-alanine, and from about 20 mM to about 200 mM glutamic acid.
Further we disclose here a separation medium for capillary electrophoretic size separation of proteins, wherein said acid buffer comprises from about 20 mM to about 200 mM γ-aminobutyric acid, and from about 20 mM to about 200 mM glutamic acid.
We also disclose here a separation medium for capillary electrophoretic size separation of proteins, wherein said acid buffer comprises from about 20 mM to about 200 mM β-alanine and from about 20 mM to about 200 mM 2-hydroxyisobutyric acid.
Further we disclose here a separation medium for capillary electrophoretic size separation of proteins, consisting essentially of:
We disclose here a separation medium for capillary electrophoretic size separation of proteins, consisting essentially of 15 g/L polyacrylamide having molecular weight 600,000-1 million, 100 mM β-alanine, 100 mM glutamic acid, and 1 g/L cetyldimethylethylammonium bromide.
Further we disclose here a separation medium for capillary electrophoretic size separation of proteins, consisting essentially of:
We disclose here a separation medium for capillary electrophoretic size separation of proteins, consisting essentially of 100 g/L dextran having molecular weight 2,000,000, 100 mM γ-aminobutyric acid, 100 mM glutamic acid, and 2 g/L cetyldimethylethylammonium chloride.
Further we disclose here a method for capillary electrophoretic size separation of proteins, comprising steps:
We also disclose here a method for capillary electrophoretic size separation of proteins, wherein said sieving polymer in said separation medium for capillary electrophoretic size separation of proteins is about 100 g/L dextran with molecular weight of about 2 million.
Further we disclose here a method for capillary electrophoretic size separation of proteins, wherein said sieving polymer in said separation medium for capillary electrophoretic size separation of proteins is from about 8 g/L to about 30 g/L polyacrylamide, having molecular weight from about 100,000 to about 1,000,000.
The separations described in these examples were performed in 3D CE capillary electrophoresis instrument at 20° C. in a bare or coated capillary of internal diameter 75 μm and outer diameter 360 vm with UV detection at 214 nm.
Several compositions of the sample denaturing solution were formulated to enable protein quantitation with electrokinetic injection:
During the sample preparation, proteins were dissolved in the sample denaturing solution and incubated at 90° C. for 2 min. Proteins were also successfully denatured by incubation at 70° C. for 10 min. Some proteins, e.g., lysozyme, were resistant to the thermal denaturation with cationic surfactants and an extended incubation at high temperature (95° C.) was necessary for some formulations (5 min in case of lysozyme). Proteins such as BSA, on the other hand, did not require any denaturation at all prior to electrophoresis.
Capillary sieving electrophoresis with a cationic surfactant was performed in bare or coated fused silica capillary (U.S. Pat. No. 7,799,195), 75 μm ID, 360 μm OD, 335 mm total length, 250 mm effective length. In bare capillaries, poly(ethylene oxide) (PEO) was usually a sieving polymer, but for high-resolution separations, capillaries with internal hydrophilic coating were preferred. After each electrophoretic run, the capillary was flushed with 100 mM citric acid at pressure of 930 mbar for 7 min to remove the sieving matrix from the previous run and wash away proteins and other material potentially adsorbed on the capillary wall. In the next step, the capillary was prepared for the next run: the fresh sieving matrix was pumped into the capillary with pressure of 930 mbar for 3 min. The samples were injected either electrokinetically or by pressure. The amount of the injected sample depended on the protein concentration in the sample. The samples prepared with the sample denaturing solution containing 10 g/L CDMEAB, 100 mM KCl, and 10 g/L dithiotreitol and containing 0.1-1 g/L proteins were typically injected for 8 s at 6 kV. The separation was performed at +10 kV and typically took 10-12 minutes. The separation of a model protein mixture is shown in
The electrophoretic mobility of the proteins was plotted against the logarithmic molecular weight to provide calibration curve for determination of protein molecular weight (
The method of capillary sieving electrophoresis in bare capillary with non coating polymer
Capillary sieving electrophoresis with a cationic surfactant was performed in a bare capillary, 75 μm ID, 360 μM OD, 335 mm total length, 250 mm effective length. For separations in bare capillaries with linear polyacrylamide or dextran as sieving polymers, the bare capillary had to be flushed with a PEO solution before filling with the separation medium.
After each electrophoretic run, the capillary was flushed with 400 mM β-alanine, 400 mM 2-hydroxisobutyric acid. at pressure of 930 mbar for 7 min to remove the sieving matrix from the previous run and wash off proteins and other material potentially adsorbed on the capillary wall. In the next step, the capillary was flushed with 10 g/L poly(ethylene oxide) (Mw 7,000,000), 400 mM β-alanine, 400 mM 2-hydroxisobutyric acid to suppress electroosmotic flow. Then a fresh sieving matrix was pumped into the capillary with pressure of 930 mbar for 3 min. The sieving matrix contained 100 mM β-alanine, 100 mM 2-hydroxisobutyric acid, 2 g/L CTAC, and 50 g/L dextran (Mw 2,000,000). Samples containing 0.8 g/L model proteins in 100 mM KCl, 10 g/L CTAC, 4 g/L tris-(carboxyethyl)phosphine were electrokinetically injected by applying voltage 8 kV for 15 s.
Proteins were separated at voltage of +10 kV and detected by measuring UV absorbance at 214 nm (
For the separation of native BSA oligomers, the sample containing 10 g/L BSA in 1 g/L CDMEAB was injected at 8 kV for 15 s. The capillary sieving electrophoresis (CSE) of BSA oligomers took about 12 min. and revealed eight to nine peaks (
CSE with a cationic surfactant provided narrow peaks with high separation efficiency. Table 1 summarizes the average separation efficiency of model proteins from 7 runs. The calculation of the separation efficiency from a half-height peak width that assumed ideal Gaussian peaks provided results rather lower than the calculation based on an unrevealed algorithm used in ChemStation software (Agilent).
acalculated from half-height peak width
bobtained directly from the ChemStation software (Agilent)
10 overlaid consecutive electropherograms of a model mixture containing 0.8 g/L of insulin B, lysozyme, β-lactoglobulin, α-chymotrypsinogen A, ovalbumin, and BSA are shown in
CSE with cationic surfactant allowed quantitative analysis with electrokinetic injection. When proteins were denatured in 10 g/L CDMEAB, 100 mM KCl, and 10 g/L DTT and injected 30 s at +10 kV, the calibration lines for lysozyme, β-lactoglobulin, ovalbumin, and BSA were linear in the concentration range 0-1.0 g/L (
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| Number | Date | Country | |
|---|---|---|---|
| Parent | 12359345 | Jan 2009 | US |
| Child | 12359345 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 12359345 | Jan 2009 | US |
| Child | 13350793 | US |
