Carbon substituted aminothiazole inhibitors of cyclin dependent kinases

Abstract

Compounds of the formula 1

Description

BRIEF DESCRIPTION OF THE INVENTION

[0002] The present invention is directed to compounds of the formula 4

[0003] and pharmaceutically acceptable salts thereof. As used in formula I, and throughout the specification, the symbols have the following meanings:

[0004] R1═R2, COR3, CONH2, CONR2R3, COOR2, or SO2R2;

[0005] R2=alkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl;

[0006] R3═H, alkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl; 5

[0007] , where n=0,1,2; m=1,2 but both n and m cannot be 2, or

[0008] , where i, j=0 or 1 but cannot both be 1, and Y=optionally substituted alkene, alkyne, or any 2 adjacent carbon atoms of a cycloalkyl or cycloheteroalkyl ring of 3-7 atoms;

[0009] R4=alkyl with two or more carbon atoms, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl, or R9, with the proviso that when R1 is acetyl or propionyl and Y=alkene, then R4 cannot be nitrofuryl or 2-quinolinyl;

[0010] R5, R6, R7, R8=independently H, alkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl, halo, or hydroxy, alkoxy, amino, NR12R13, thio, or alkylthio, with the proviso that only one such heteroatom group is bonded to any one carbon atom; 6

[0011] where Z═O, NR14, S;

[0012] R10, R11=independently H, alkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl, halo, hydroxy, alkoxy, alkylcarbonyloxy, carboxy, alkyloxycarbonyl, amino, NR15R16, carbamoyl, ureido, thio, or alkylthio;

[0013] R12, R13, R14, R15, R16=independently H, alkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl.

[0014] The compounds of formula I are protein kinase inhibitors and are useful in the treatment of proliferative diseases, for example, cancer, inflammation and arthritis. They may also be useful in the treatment of Alzheimer's disease, and cardiovascular disease.

DESCRIPTION OF THE INVENTION

[0015] The present invention provides for compounds of formula I, pharmaceutical compositions employing such compounds, and for methods of using such compounds.

[0016] Listed below are definitions of various terms used to describe the compounds of the instant invention. These definitions apply to the terms as they are used throughout the specification (unless they are otherwise limited in specific instances) either individually or as part of a larger group.

[0017] It should be noted that any heteroatom with unsatisfied valances is assumed to have the hydrogen atom to satisfy the valances.

[0018] Carboxylate anion refers to a negatively charged group —COO−.

[0019] The term “alkyl” or “alk” refers to a monovalent alkane (hydrocarbon) derived radical containing from 1 to 12 carbon atoms unless otherwise defined. An alkyl group is an optionally substituted straight, branched or cyclic saturated hydrocarbon group. When substituted, alkyl groups may be substituted with up to four substituent groups, R as defined, at any available point of attachment. When the alkyl group is said to be substituted with an alkyl group, this is used interchangeably with “branched alkyl group”. Exemplary unsubstituted such groups include methyl, ethyl, propyl, isopropyl, n-butyl, t-butyl, isobutyl, pentyl, hexyl, isohexyl, heptyl, 4,4-dimethylpentyl, octyl, 2,2,4-trimethylpentyl, nonyl, decyl, undecyl, dodecyl, and the like. Exemplary substituents may include but are not limited to one or more of the following groups: halo (such as F, Cl, Br, I), haloalkyl (such as CCl3 or CF3), alkoxy, alkylthio, hydroxy, carboxy (—COOH), alkyloxycarbonyl (—COOR), alkylcarbonyloxy (—OCOR), amino (—NH2), carbamoyl (—NHCOOR— or —OCONHR—), urea (—NHCONHR—), amidinyl (—CNHNHR or —CNRNH2), or thiol (—SH). Alkyl groups as defined may also comprise one or more carbon to carbon double bonds or one or more carbon to carbon triple bonds.

[0020] The term “alkenyl” refers to a hydrocarbon radical straight, branched or cyclic containing from 2 to 12 carbon atoms and at least one carbon to carbon double bond.

[0021] The term “alkynyl” refers to a hydrocarbon radical straight, branched or cyclic containing from 2 to 12 carbon atoms and at least one carbon to carbon triple bond.

[0022] Cycloalkyl is a specie of alkyl containing from 3 to 15 carbon atoms, without alternating or resonating double bonds between carbon atoms. It may contain from 1 to 4 rings. Exemplary unsubstituted such groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, etc. Exemplary substituents include one or more of the following groups: halogen, alkyl, alkoxy, alkyl hydroxy, amino, nitro, cyano, thiol and/or alkylthio.

[0023] The terms “alkoxy” or “alkylthio”, as used herein, denote an alkyl group as described above bonded through an oxygen linkage (—O—) or a sulfur linkage (—S—), respectively.

[0024] Sulfoxide and sulfone denote groups bonded by —SO— or —SO2— linkages, respectively.

[0025] The term “alkyloxycarbonyl”, as used herein, denotes an alkoxy group bonded through a carbonyl group. An alkoxycarbonyl radical is represented by the formula: —C(O)OR, where the R group is a straight or branched C1-6 alkyl group.

[0026] The term “alkylcarbonyl” refers to an alkyl group bonded through a carbonyl group.

[0027] The term “alkylcarbonyloxy”, as used herein, denotes an alkylcarbonyl group which is bonded through an oxygen linkage.

[0028] The term “arylalkyl”, as used herein, denotes an aromatic ring bonded to an alkyl group as described above.

[0029] The term “aryl” refers to monocyclic or bicyclic aromatic rings, e.g. phenyl, substituted phenyl and the like, as well as groups which are fused, e.g., napthyl, phenanthrenyl and the like. An aryl group thus contains at least one ring having at least 6 atoms, with up to five such rings being present, containing up to 22 atoms therein, with alternating (resonating) double bonds between adjacent carbon atoms or suitable heteroatoms. Aryl groups may optionally be substituted with one or more groups including, but not limited to halogen, alkyl, alkoxy, hydroxy, carboxy, carbamoyl, alkyloxycarbonyl, nitro, trifluoromethyl, amino, cycloalkyl, cyano, alkyl S(O)m (m=, 0,1, 2), or thiol.

[0030] The term “heteroaryl” refers to a monocyclic aromatic hydrocarbon group having 5 or 6 ring atoms, or a bicyclic aromatic group having 8 to 10 atoms, containing at least one heteroatom, O, S, or N, in which a carbon or nitrogen atom is the point of attachment, and in which one or two additional carbon atoms is optionally replaced by a heteroatom selected from O or S, and in which from 1 to 3 additional carbon atoms are optionally replaced by nitrogen heteroatoms, said heteroaryl group being optionally substituted as described herein. Exemplary heteroaryl groups include the following: thienyl, furyl, pyrrolyl, pyridinyl, imidazolyl, pyrrolidinyl, piperidinyl, thiazolyl, oxazolyl, triazolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrazinyl, tetrazolyl, pyridazinyl, pyrimidinal, triazinylazepinyl, indolyl, isoindolyl, quinolinyl, isoquinolinyl, benzothiazolyl, benzoxazolyl, benzimidazolyl, benzoxadiazolyl, benzofurazanyl and tetrahydropyranyl. Exemplary substituents include one or more of the following: halogen, alkyl, alkoxy, hydroxy, carboxy, carbamoyl, alkyloxycarbonyl, trifluoromethyl, cycloalkyl, nitro, cyano, amino, alkylS(O)m (m=, 0,1, 2), or thiol.

[0031] The term “heteroarylium” refers to heteroaryl groups bearing a quaternary nitrogen atom and thus a positive charge.

[0032] The term “heterocycloalkyl” refers to a cycloalkyl group (nonaromatic) in which one of the carbon atoms in the ring is replaced by a heteroatom selected from O, S or N, and in which up to three additional carbon atoms may be replaced by said heteroatoms.

[0033] The term “quaternary nitrogen” refers to a tetravalent positively charged nitrogen atom including, e.g. the positively charged nitrogen in a tetraalkylammonium group (e.g. tetramethylammonium, N-methylpyridinium), the positively charged nitrogen in protonated ammonium species (e.g. trimethylhydroammonium, N-hydropyridinium), the positively charged nitrogen in amine N-oxides (e.g. N-methyl-morpholine-N-oxide, pyridine -N-oxide), and the positively charged nitrogen in an N-amino-ammonium group (e.g. N-aminopyridinium).

[0034] The term “heteroatom” means O, S or N, selected on an independent basis.

[0035] The term “halogen” or “halo” refers to chlorine, bromine, fluorine or iodine.

[0036] When a functional group is termed “protected”, this means that the group is in modified form to preclude undesired side reactions at the protected site. Suitable protecting groups for the compounds of the present invention will be recognized from the present application taking into account the level of skill in the art, and with reference to standard textbooks, such as Greene, T. W. et al., Protective Groups in Organic Synthesis, Wiley, N.Y. (1991).

[0037] Suitable examples of salts of the compounds according to the invention with inorganic or organic acids are hydrochloride, hydrobromide, sulfate, phosphate. Salts which are unsuitable for pharmaceutical uses but which can be employed, for example, for the isolation or purification of free compounds I or their pharmaceutically acceptable salts, are also included.

[0038] All stereoisomers of the compounds of the instant invention are contemplated, either in admixture or in pure or substantially pure form. The definition of the compounds according to the invention embraces all possible stereoisomers and their mixtures. It very particularly embraces the racemic forms and the isolated optical isomers having the specified activity. The racemic forms can be resolved by physical methods, such as, for example, fractional crystallization, separation or crystallization of diastereomeric derivatives or separation by chiral column chromatography. The individual optical isomers can be obtained from the racemates by conventional methods, such as, for example, salt formation with an optically active acid followed by crystallization.

[0039] All configurational isomers of compounds of the present invention are contemplated, either in admixture or in pure or substantially pure form. The definition of compounds of the present invention very particularly embraces both cis (Z) and trans (E) alkene isomers, as well as cis and trans isomers of cycloalkyl or heterocycloalkyl rings.

[0040] It should be understood that solvates (e.g. hydrates) of the compounds of formula I are also within the scope of the present invention. Methods of salvation are generally known in the art. Accordingly, the compounds of the instant invention may be in the free or hydrate form, and may be obtained by methods exemplified by the following schemes.

[0041] The synthesis of compounds of formula I can proceed through the known aldehyde of formula II (Scheme 1) which was prepared according to the procedures set forth in II Farmaco 44, 1011, (1989) and the references therein. Treatment of II with either (R2CO)2O or R1—L, where L is a leaving group such as a halogen or sulfonate ester, yields compounds of formula III. Condensation of formula III with phosphorus-stabilized anions such as the phosphonate of formula IV or a Wittig reagent in the presence of base yields compounds of formula V (that is, compounds of formula I where A contains an alkene present as either the cis or trans isomer). Alternatively, compounds of formula V may be prepared by first reacting formula II with the phosphonate of formula IV or a Wittig reagent in the presence of base, and then treating the resulting product with (R2CO)2O or R1—L. 7

[0042] Compounds of formula V may be converted into other compounds of formula I as shown in Scheme 2. For example, treatment of compounds of formula V with agents such as H2 on Pd/C yields the saturated compounds of formula VI (which is a compound of formula I. Alternatively compounds of formula V may be epoxidized with agents such as dimethyldioxirane or m-chloroperbenzoic acid to yield epoxides of formula VII (which are compounds of formula I where Y=the carbon atoms of oxirane). Cyclopropanation of the olefin with agents such as ZnCuCH2 or diazomethane may yield cyclopropanes of formula VIII (which are compounds of formula I where Y=cyclopropane). 8

[0043] Aldehydes of formula III may also be converted into compounds of formula I which have R7 or R8 groups containing oxygen (Scheme 3). For example, addition of organometallic reagents of formula R*-M, where R*═R4(R5R6C)i— or R4(R5R6C)I—Y— and M=a metal, would yield compounds of formula IX (that is, compounds of formula I where R7=hydroxy and R8═H). Alkylation of the hydroxyl group in compounds of formula IX using W—L, where W=alkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl, and L is a leaving group such as a halogen or sulfonate ester, would yield ethers of formula X (that is, compounds of formula I with R7=alkoxy). 9

[0044] Scheme 4 outlines a procedure that may be used for the solid phase synthesis of compounds of formula I. A benzyl chloride resin, such as that depicted by formula XII, may be alkylated by an aminothiazole of formula III (where R1═CF3CO) to give a compound of formula XII. Coupling with phosphorus stabilized anions such as compounds of formula IV will yield alkenes of formula XIII which may be deprotected by a reducing agent such as sodium borohydride, or a base such as sodium hydroxide, to give amines of formula XIV. The amines of formula XIV may react with R1—L or (R2CO)2O to give compounds of formula XV, which may be cleaved from the resin with trifluoroacetic acid to give compounds of formula V (which are compounds of formula I where Y is an alkene). Compounds of formula IX or X may also be synthesized on solid phase using analogous chemistry to that shown in Scheme 3 by starting with aldehyde XII. 10

[0045] Compounds of formula I wherein R4═R9 may be synthesized from aldehydes of formula III (Scheme 5). These aldehydes may be reduced with agents such as sodium borohydride to give alcohols of formula XVI which may be converted into a compound of formula XVII, where L is a leaving group such as a halogen or sulfonate ester, by treatment with agents such as p-toluenesulfonyl chloride and base or thionyl chloride. The anion of dialkylmalonate esters of formula XVIII may be alkylated by compounds of formula XVII to form diesters of formula XIX, where W=alkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl. These diesters may be saponified and decarboxylated to form acids of formula XX which may be coupled with amines of formula XXI to give amides of formula XXII. These amides may be cyclized upon exposure to dehydrating agents such as POCl3 to form compounds of formula VI which are compounds of formula I where Z═O. 11

[0046] Compounds of formula I wherein R4=R9 and Y=alkynyl or Z-alkenyl may be prepared from halomethyl oxazoles such as XXIII (Scheme 6). Displacement of the chlorine to give the acetate XXIV, followed by basic hydrolysis and oxidation provides a 2-oxazolyl aldehyde XXVI. The aldehyde may be treated with a reagent such as carbon tetrabromide and triphenylphosphine to give a dibromo olefin XXVII. Elimination of HBr by strong base, followed by lithiation and quenching the acetylenic anion with tributyltin chloride gives an acetylenic stannane XXVIII, which may be coupled with a 2-iodo aminothiazole XXIX, to give XXX, which is a compound of formula I wherein R4═R9 and Y is alkynyl. The acetylenic compounds of formula XXX may be hydrogenated to provide cis olefins XXXI and XXXIII, which are compounds of formula I wherein R4═R9 and Y is Z-alkenyl. 12

[0047] Compounds of formula I wherein R1=R2 may be prepared by the methods shown in Scheme 7. 5-Formyl-2-aminothiazole II is reacted with a nitrosating agent such as tBuONO and CuBr2 to form the 2-bromo derivative XXXIV. Following procedures as described in Scheme I, the aldehyde is reacted with a phosphonate of formula IV or a Wittig reagent in the presence of base to provide an olefin of formula XXXV. Reaction of the 2-bromo olefin of formula XXXV with a compound of formula R2NH2 in the presence of a base such as sodium hydride gives compounds of formula, which is a compound of formula I wherein R1=R2 and Y is alkenyl. 13

[0048] Alternatively, compounds of formula XXXVI where R1═R2 and Y is alkenyl may be prepared according to Scheme 8. The amino group of compound II may be protected with a reagent such as di-t-butyl dicarbonate to give XXXVII, followed by reaction with a phosphonate of formula IV or a Wittig reagent in the presence of base such as an alkoxide or sodium hydride to give a compound of formula XXXVIII. Treatment of XXXVIII with R2L where L is a leaving group such as halo or sulfonate, in the presence of base, followed by removal of the protecting group gives a compound of formula XXXVI, which is a compound of formula I where R1═R2 and Y is alkenyl. 14

[0049] Compounds of formula R4CH2P(O)(OEt)2 may be prepared from compounds of formula R4CH2L, where L is a leaving group such as halogen or sulfonate ester, by heating with triethylphosphite. Compounds of formula R9-L, where Z═O, may be prepared from LCH2CN and R11C(N2)COR10, according to part E of Example 2.

[0050] The starting compounds of Schemes 1-7 are commercially available or may be prepared by methods known to one of ordinary skill in the art.

[0051] All compounds of formula I may be prepared by modification of the procedures described herein.

[0052] Preferred compounds of formula I are those where:

[0053] R1═R2, COR3, or CONR2R3;

[0054] R2═alkyl, aryl, or heteroaryl;

[0055] R3═H, alkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl; 15

[0056] , where n=0, 1, 2; m=1, 2, or

[0057] , where i, j=0 or 1 but cannot both be 1, and Y=optionally substituted alkene, alkyne, or any two adjacent carbon atoms of a cycloalkyl ring;

[0058] R4=alkyl with two or more carbon atoms, aryl, heteroaryl, or R9;

[0059] R5, R6, R7, R8=independently H, or alkyl; 16

[0060] where Z═O;

[0061] R10, R11=independently H, or alkyl.

[0062] The compounds according to the invention have pharmacological properties; in particular, the compounds of formula I are inhibitors of protein kinases such as the cyclin dependent kinases (cdks), for example, cdc2 (cdk1), cdk2, and cdk4. The novel compounds of formula I are expected to be useful in the therapy of proliferative diseases such as cancer, inflammation, arthritis, Alzheimer's disease and cardiovascular disease. These compounds may also be useful in the treatment of topical and systemic fungal infections.

[0063] More specifically, the compounds of formula I are useful in the treatment of a variety of cancers, including (but not limited to) the following:

[0064] carcinoma, including that of the bladder, breast, colon, kidney, liver, lung, ovary, pancreas, stomach, cervix, thyroid, prostate, and skin;

[0065] hematopoietic tumors of lymphoid lineage, including acute lymphocytic leukemia, B-cell lymphoma, and Burkett's lymphoma;

[0066] hematopoietic tumors of myeloid lineage, including acute and chronic myelogenous leukemias and promyelocytic leukemia;

[0067] tumors of mesenchymal origin, including fibrosarcoma and rhabdomyosarcoma; and

[0068] other tumors, including melanoma, seminoma, teratocarcinoma, osteosarcoma, neuroblastoma and glioma.

[0069] Due to the key role of cdks in the regulation of cellular proliferation in general, inhibitors could act as reversible cytostatic agents which may be useful in the treatment of any disease process which features abnormal cellular proliferation, e.g., neuro-fibromatosis, atherosclerosis, pulmonary fibrosis, arthritis, psoriasis, glomerulonephritis, restenosis following angioplasty or vascular surgery, hypertrophic scar formation, inflammatory bowel disease, transplantation rejection, angiogenesis, and endotoxic shock.

[0070] Compounds of formula I may also be useful in the treatment of Alzheimer's disease, as suggested by the recent finding that cdk5 is involved in the phosphorylation of tau protein (J. Biochem, 117, 741-749 (1995)).

[0071] Compounds of formula I may also act as inhibitors of other protein kinases, e.g., protein kinase C, her2, rafl, MEK1, MAP kinase, EGF receptor, PDGF receptor, IGF receptor, Pl3 kinase, wee1 kinase, Src, Abl, and thus be effective in the treatment of diseases associated with other protein kinases.

[0072] The compounds of this invention may also be useful in combination with known anti-cancer treatments such as radiation therapy or with cytostatic and cytotoxic agents, such as for example, but not limited to, DNA interactive agents, such as cisplatin or doxorubicin; inhibitors of farnesyl protein transferase, such as those described in pending U.S. application Ser. No. 08/802,239 which was filed on Feb. 20, 1997; topoisomerase II inhibitors, such as etoposide; topoisomerase I inhibitors, such as CPT-11 or topotecan; tubulin stabilizing agents, such as paclitaxel, docetaxel or the epothilones; hormonal agents, such as tamoxifen; thymidilate synthase inhibitors, such as 5-fluorouracil; and antimetabolites, such as methoxtrexate; antiangiogenic agents, such as angiostatin; and kinase inhibitors, such as her2 specific antibodies.

[0073] If formulated as a fixed dose, such combination products employ the compounds of this invention within the dosage range described below and the other pharmaceutically active agent within its approved dosage range. For example, the cdc2 inhibitor olomucine has been found to act synergistically with known cytotoxic agents in inducing apoptosis (J. Cell Sci., 108, 2897 (1995)). Compounds of formula I may be used sequentially with known anti-cancer or cytotoxic agents when a combination formulation is inappropriate.

[0074] cdc2/cyclin B1 Kinase Assay

[0075] cdc2/cyclin B1 kinase activity was determined by monitoring the incorporation of 32P into histone HI. The reaction consisted of 50 ng baculovirus expressed GST-cdc2, 75 ng baculovirus expressed GST-cyclin B1, 1 μg histone HI (Boehringer Mannheim), 0.2 μCi of 32P γ-ATP and 25 μM ATP in kinase buffer (50 mM Tris, pH 8.0, 10 mM MgCl2, 1 mM EGTA, 0.5 mM DWF). The reaction was incubated at 30° C. for 30 minutes and then stopped by the addition of cold trichloroacetic acid (TCA) to a final concentration of 15% and incubated on ice for 20 minutes. The reaction was harvested onto GF/C unifilter plates (Packard) using a Packard Filtermate Universal harvester, and the filters were counted on a Packard TopCount 96-well liquid scintillation counter (Marshak, D. R., Vanderberg, M. T., Bae, Y. S., Yu, I. J., J. of Cellular Biochemistry, 45, 391-400 (1991), incorporated by reference herein).

[0076] cdk2/cyclin E Kinase Assay

[0077] cdk2/cyclin E kinase activity was determined by monitoring the incorporation of 32P into the retinoblastoma protein. The reaction consisted of 2.5 ng baculovirus expressed GST-cdk2/cyclin E, 500 ng bacterially produced GST-retinoblastoma protein (aa 776-928), 0.2 μCi 32P γ-ATP and 25 μM ATP in kinase buffer (50 mM Hepes, pH 8.0, 10 mM MgCl2, 5 mM EGTA, 2 mM DTT). The reaction was incubated at 30° C. for 30 minutes and then stopped by the addition of cold trichloroacetic acid (TCA) to a final concentration of 15% and incubated on ice for 20 minutes. The reaction was harvested onto GF/C unifilter plates (Packard) using a Packard Filtermate Universal harvester, and the filters were counted on a Packard TopCount 96-well liquid scintillation counter.

[0078] cdk 4/cyclin D1 Kinase Activity

[0079] cdk4/cyclin D1 kinase activity was determined by monitoring the incorporation of 32P in to the retinoblastoma protein. The reaction consisted of 165 ng baculovirus expressed as GST-cdk4, 282 ng bacterially expressed as S-tag cyclin D1, 500 ng bacterially produced GST-retinoblastoma protein (aa 776-928), 0.2 μCi 32P γ-ATP and 25 μM ATP in kinase buffer (50 mM Hepes, pH 8.0, 10 mM MgCl2, 5 mM EGTA, 2 mM DTT). The reaction was incubated at 30° C. for 1 hour and then stopped by the addition of cold trichloroacetic acid (TCA) to a final concentration of 15% and incubated on ice for 20 minutes. The reaction was harvested onto GF/C unifilter plates (Packard) using a Packard Filtermate Universal harvester, and the filters were counted on a Packard TopCount 96-well liquid scintillation counter (Coleman, K. G., Wautlet, B. S., Morissey, D, Mulheron, J. G., Sedman, S., Brinkley, P., Price, S., Wedster, K. R. (1997) Identification of CDK4 Sequences involved in cyclin D, and p16 binding. J. Biol. Chem. 272,30:18869-18874, incorporated by reference herein).

[0080] The following examples and preparations describe the manner and process of making and using the invention and are illustrative rather than limiting. It should be understood that there may be other embodiments which fall within the spirit and scope of the invention as defined by the claims appended hereto.

EXAMPLE 1

[0081] 17

A. Preparation of 2-acetamido-5-bromothiazole

[0082] To a solution of 2-amino-5-bromothiazole (22.3 g, 85.9 mmol) in methylene chloride (100 mL) and pyridine (60 mL) was added acetic anhydride (11 mL) slowly with stirring. The mixture was allowed to stir for 2.5 hours, warmed to room temperature, and stirred for an additional 4 hours. Most of the solvent was removed in vacuo and the residue was washed with ethyl acetate and aqueous HCl. The organic solution was then washed with water, dried over MgSO4 and concentrated to give a crude solid. This solid was triturated with Et2O, filtered, washed with Et2O, and dried to give 2-acetamido-5-bromothiazole as a solid (15.1 g, 80%, C5H5BrN2OS, MS m/e 222 (M+H)+).

B. Preparation of ethyl 3-((E)-2-acetamido-thiazol-5-yl)-acrylate

[0083] A mixture of acetamido-5-bromothiazole (440 mg, 2.0 mmol), ethyl acrylate (400 mg, 4 mmol) and triethylamine (3 mL) in DMF (3 mL) was stirred at 90° C. under argon in the presence of Pd(tol3P)2Cl2 (150.0 mg) for 24 h. The mixture was concentrated and the residue was dissolved in methylene chloride (100 mL), washed with water and dried over MgSO4. The solution was concentrated and the residue was purified by column chromatography (SiO2, CH2Cl2:MeOH/100:5) to afford ethyl 3-((E)-2-acetamido-thiazol-5-yl)-acrylate (100 mg, 21%) as a solid (m.p. 239-240° C., C10H12N2O3S, MS m/e 240.9 (M+H)+).

EXAMPLE 2

[0084] 18

A. Preparation of 2-amino-thiazol-5-ylcarboxaldehyde

[0085] 2-Amino-thiazol-5-ylcarboxaldehyde was synthesized according to the procedure set forth in II Farmaco 44, 1011, (1989) and the references therein.

B. Preparation of 2-acetamido-thiazol-5-ylcarboxaldehyde

[0086] To a suspension of 2-amino-thiazol-5-ylcarboxaldehyde (5.0 g, 39 mmol) in toluene (500 mL) was added acetic anhydride (11.0 mL, 117 mmol). The mixture was heated to 110° C. for 5 hours. Upon cooling to room temperature, a solid precipitated out of the solution. The reaction mixture was concentrated under vacuum to give 2-acetamido-thiazol-5-ylcarboxaldehyde as a light brown colored solid (6.5 g, 98%, C6H6N2O2S, MS m/e 171 (M+H)+).

C. Preparation of Diazomethane

[0087] Caution: diazomethane is potentially explosive. Care should be taken to use plastic containers, or glassware free of scratches. Solid KOH (60 g) was dissolved in water to make 150 mL of a 40% KOH solution. This solution was cooled at 0° C. and ether (500 mL) was added. To this cooled mixture was added 1-methyl-3-nitro-1-nitrosoguanidine (50 g, 0.34 mol) in portions over 45 minutes. After addition was complete, the ether layer was decanted and to give a solution of diazomethane which was used directly.

D. Preparation of 1-diazo-3,3-dimethyl-2-butanone

[0088] To the diazomethane solution was added a solution of trimethylacetyl chloride (15 mL, 0.12 mol) in ether (100 mL) dropwise over 40 minutes. After addition was complete, the solution was allowed to warm slowly overnight to room temperature. The solution was purged with a flow of nitrogen gas to remove any excess diazomethane and the resulting solution was concentrated to give 1-diazo-3,3-dimethyl-2-butanone as a yellow oil which was used directly in the next step.

E. Preparation of 2-(chloromethyl)-5-t-butyloxazole

[0089] To a stirred solution of chloroacetonitrile (40 mL) and boron trifluoride etherate (20 mL, 0.16 mmol) at 0° C. was added 1-diazo-3,3-dimethyl-2-butanone in chloroacetonitrile (40 mL) dropwise over a period of 20 minutes. After addition was complete, the mixture was stirred at 0° C. for one hour and then partitioned between saturated NaHCO3, solution (700 mL) and CH2Cl2 (500 mL). The aqueous solution was extracted with CH2Cl2 (500 mL) and the combined organic layers were washed with brine (400 mL) and dried over MgSO4. After filtration, the solution was concentrated and then distilled under vacuum using an oil bath temperature of 40° C. The 2-(chloromethyl)-5-t-butyloxazole (9.2 g, 44% overall from the acid chloride, C8H12ClNO, MS m/e 174 (M+H)+) was obtained as a light yellow oil.

F. Preparation of (5-t-butyl-oxazol-2-ylmethyl)-phosphonic acid diethyl ester

[0090] A solution of 2-(chloromethyl)-5-t-butyloxazole (8.00 g, 46.1 mmol) in triethylphosphite (15.3 g, 92.0 mmol) was heated at 120° C. for 18 hours. After cooling the mixture to room temperature, toluene (30 mL) was added and the solution was concentrated in vacuo with a bath temperature of 70° C. This procedure was repeated three times and the resulting brown oil was dried in vacuo at 90° C. for 30 minutes to give (5-t-butyl-oxazol-2-ylmethyl)-phosphonic acid diethyl ester (12.4 g, 98%, C12H22NO4P, MS m/e 276 (M+H)+) as a red-orange liquid.

G. Preparation of N-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-acetamide

[0091] To a solution of (5-butyl-oxazol-2-ylmethyl)-phosphonic acid diethyl ester (195 mg, 0.708 mmol) in tetrahydrofuran (10 mL) was added potassium t-butoxide (2.2 mL, 1 M in THF) via syringe. After 10 minutes, a solution of 2-acetamido-thiazo-5-ylcarboxaldehyde (100 mg, 0.587 mmol) in THF (6 mL) was added via syringe. Over the course of a half-hour, a precipitate formed in the solution. Methanol (1.5 mL) was added to dissolve the precipitate, and after an additional half-hour, the reaction was concentrated under reduced pressure to form a slurry. This was diluted with CHCl3 (50 mL) and quenched with water (25 mL). The solution was extracted with CHCl3 (3×50 mL) and ethyl acetate (3×50 mL) until all the formed solid was in solution. The combined organic layers were washed with water (50 mL) and dried over brine followed by MgSO4 and then concentrated to give a yellow solid. The crude solid was purified by chromatography (SiO2, 5% MeOH/CHCl3) to afford N-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-acetamide (118 mg, 69%, m.p. 275° C., C14H17N3O2S, MS m/e 292 (M+H)+) as a light yellow solid. HPLC-HI 100% at 3.95 min (YMC S5 ODS coulm 4.6×50 mm, 10-90% aqueous methanol over 4 minutes containing 0.2% phosphoric acid, 4 mL/min, monitoring at 220 nm).

EXAMPLE 3

[0092] 19

A. Preparation of N-[5-(2-(5-t-butyl-oxazol-2-yl)-ethyl)-thiazol-2-yl]-acetamide

[0093] A solution N-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]acetamide (67 mg, 0.23 mmol) in ethyl acetate (10 mL) was added to a suspension of pre-reduced Pd/C (10%) in ethyl acetate (5 mL). The reaction flask was fitted with a hydrogen balloon and stirred for 24 hours. The reaction was filtered, and the mixture was resubmitted to hydrogenation using the same conditions as above. After an additional 24 hours, the reaction was filtered through a plug of celite, concentrated, and purified by chromatography (SiO2, 5% MeOH/CHCl3) to give N-[5-(2-(5-t-butyl-oxazol-2-yl)-ethyl)-thiazol-2-yl]-acetamide as a white solid (3.5 mg, 5%, C14H19N3O2S, MS m/e 294 (M+H)+). HPLC-HI 91% at 6.75 min (Zorbax SB C18 column 4.6×75 mm, 10-90% aqueous methanol over 8 minutes containing 0.1% TFA, 2.5 mL/min, monitoring at 220 nm).

EXAMPLE 4

[0094] 20

A. Preparation of [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-carbamic acid t-butyl ester

[0095] A sample of [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-carbamic acid t-butyl ester could be prepared according to the methods described in Example 2.

B. Preparation of 2-amino-5-[(E)-2-(5-t-butyl-oxazol-2-yl)-vinyl]-thiazole

[0096] To a suspension of [5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazo-2-yl]-carbamic acid t-butyl ester (1.5 g, 4.3 mmol) in tetrahydrofuran (30 mL) and water (3 mL) was added concentrated HCl (3 mL) dropwise. After addition was complete, the mixture was heated at 60° C. overnight. The solution was concentrated in vacuo to give a slurry, which was neutralized with saturated aqueous NaHCO3 solution. The resulting solid was filtered and washed with water and dried to give the free base (732 mg, 68%, C12H15N3OS, MS m/e 250 (M+H)+).

C. Preparation of N-[5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-phenylacetamide

[0097] To a solution of 2-amino-5-[(E)-2-(5-t-butyl-oxazol-2-yl)-vinyl]-thiazole (20 mg, 0.08 mmol) in dry CH2Cl2 (1 mL) was added dry DMF (0.1 mL) and N,N-diisopropylethylamine (28 μL, 0.16 mmol). The resulting solution was cooled to 0° C. and phenyl acetyl chloride (21 μL, 0.16 mmol) was added via syringe. The reaction mixture was allowed to warm to room temperature over two hours and then concentrated. Chromatography (SiO2, 5% MeOH/CH2Cl2) provided a N-[5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-phenylacetamide as a mixture of Z and E isomers which were separated by reverse-phase HPLC to give the Z isomer (3 mg, 10%, MS m/e 368 (M+H)+) as a light yellow solid, and the E isomer (3 mg, 10%, C20H21N3O2S, MS m/e 368 (M+H)+) as a yellow solid. (Z)-isomer: HPLC-HI 86% at 4.05 min (YMC S5 ODS column 4.6×50 mm, 10-90% aqueous methanol over 4 minutes containing 0.1% TFA, 4 mL/min, monitoring at 254 nm). (E)-isomer: HPLC-HI 84% at 4.18 min (YMC S5 ODS column 4.6×50 mm, 10-90% aqueous methanol over 4 minutes containing 0.1% TFA, 4 mL/min, monitoring at 254 nm).

EXAMPLE 5

[0098] 21

A. Preparation of 2-hydroxymethyl-5-t-butyloxazole

[0099] To a mixture of 2-chloromethyl-5-t-butyloxazole (13.0 g, 75.1 mmole), Cs2CO3 (36.0 g, 110.5 mmole) and acetic acid (10.5 mL, 192.6 mmole) in acetonitrile (120 ml) was heated at 65 deg C. overnight. All the solvent was removed under reduced pressure, the residue was partitioned between water (60 mL) and EtOAc (100 mL) and the aqueous layer was extracted with ethyl acetate (2×100 mL). The combined EtOAc solution was dried over MgSO4 and concentrated to an oil.

[0100] The oil was dissolved in methanol (30 mL) and added with a solution of NaOH (6.50 g, 163 mmole) in 30 ml of water, and stirred at room temperature overnight. MeOH was removed under reduced pressure, and the aqueous layer was extracted with EtOAc (3×80 mL). The combined EtOAc solution was dried over MgSO4 and concentrated to give 2-hydroxymethyl-5-t-butyloxazole as oil (11.76 g, 100%).

B. Preparation of 1,1-dibromo-2-(5′-t-butyloxazol-2-yl)ethylene

[0101] To a stirred solution of oxalyl chloride (45 mL, 90 mmole) at −78 deg C. under argon was added dropwise dimethyl sulfoxide (8.80 mL, 124 mmole). The reaction mixture was stirred at −78 deg C. for 10 min., and was treated with a solution of 2-hydroxymethyl-5-t-butyloxazole (11.7 g, 75.1 mmole) in anhydrous methylene chloride (30 mL) over 20 min. The mixture was stirred at this temperature for 1 hour, then triethylamine was added slowly (31.0 mL, 222 mmole), during which the reaction mixture became a yellowish slurry. After stirring at −78 deg C. for 20 min, the reaction mixture was warmed to room temperature, added with methylene chloride (100 mL) and stirred for 1 hour. The solid was filtered off and washed with EtOAc. The filtrate was washed with 5% aqueous citric acid (100 mL) and brine (50 mL), dried over MgSO4. Concentration and column chromatography (silica gel, EtOAc/hexane 1:4) afforded 2-formyl-5-t-butyloxazole as a light yellow oil (10.1 g).

[0102] To a stirred solution of carbon tetrabromide (24.0 g, 72.4 mmole) in methylene chloride (200 mL) at 5-10 deg C. under argon atmosphere was added triphenylphosphine (37.0 g, 141 mmole) in portions. The reaction mixture was stirred for 5 min and treated with a solution of 2-formyl-5-t-butyloxazole in methylene chloride (60 mL). The reaction mixture was stirred at room temperature for 2 hours, while a white solid precipitated out of the solution. The solid was filtered off, the filtrate concentrated and purified (silica gel, EtOAc/hexane 1:4) to give 1,1-dibromo-2-(5-t-butyl-oxazol-2-yl)ethylene as a pale solid (9.13 g, 39 %).

C. Preparation of N-[5-(2-(5-t-butyl-oxazol-2-yl)-acetelenyl)-thiazol-2-yl]-acetamide

[0103] To a stirred solution of 1,1-dibromo-2-(5-t-butyl-oxazol-2-yl)ethylene (6.0 g, 19 mmole) in anhydrous THF (80 mL) at −78 deg C. under argon atmosphere. was added with 1.6 M n-butyllithium (32 mL, 51 mmole) in hexane dropwise over 20 min., the reaction mixture, stirred at −78 deg C. for 30 min and treated with tributyltin chloride (5.5 mL, 20 mmole). The reaction mixture was stirred for 30 min, warmed to 0 deg C., stirred at 0 deg C. for 30 min, and then at room temperature for 45 min. The mixture was passed through a short column of silica gel (deactivated with 2% triethylamine in hexane), and eluted with 10% EtOAc in dichloromethane to obtain crude product of 1-tributylstannyl-2-(5-t-butyl-oxazol-2-yl)acetylene as a brown oil (9.10 g).

[0104] To a stirred solution of above crude product of tin compound under argon and 2-N-acetylamino-5-iodothiazole (4.65 g, 17.3 mmole) in anhydrous THF (100 mL) at room temperature was added solid tris(dibenzylideneacetone)dipalladium(0) (1.40 g, 1.53 mmole), followed by trifurylphosphine (2.0 g, 8.6 mmole). The reaction mixture was stirred at room temperature for 10 min., then heated at 65 deg C. for 2.5 hours. The catalyst was filtered off, the filtrate concentrated and purified by column silica gel chromatography (EtOAc/hexane 1:2 to 2:1) to give N-[5-(2-(5-t-butyl-oxazol-2-yl)-ethynyl)-thiazol-2-yl]-acetamide as a light brown solid (2.60 g, 46%). C14H15N3O2S, MS m/e 290 (M+H)+. HPLC-HI 100% at 4.02 min (YMC S5 ODS column 4.6×50 mm, 10-90% aqueous methanol over 4 minutes containing 0.2% phosphoric acid, 4 mL/min, monitoring at 220 nm).

EXAMPLE 6

[0105] 22

Preparation of N-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-aminopyridine

[0106] To a solution of 2-aminopyridine (238 mg, 2.56 mmol) in THF (8 mL) under argon was added sodium hydride (100 mg of 60% oil dispersion, 2.5 mmol) and the reaction stirred at 60 deg C. for 15 minutes, cooled to room temperature and 2-bromo-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazole] (200 mg, 0.64 mmol) was added in one portion. The reaction mixture was stirred for 20 minutes, quenched with hydrochloric acid, washed with water and extracted with ethyl acetate. The organic layers were separated and concentrated to give a crude product which was heated with ethyl acetate: hexanes (1:1), cooled, filtered, and dried under vacuum to give the desired product. C17H18N4OS, MS m/e 327 (M+H)+. HPLC-HI 100% at 4.24 min (YMC S5 ODS column 4.6×50 mm, 10-90% aqueous methanol over 4 minutes containing 0.2% phosphoric acid, 4 mL/min, monitoring at 220 nm).

[0107] Using the procedures described herein or by modification of the procedures described herein as known to one or ordinary skill in the art, the following additional compounds have been prepared:

TABLE 1
				Pro-
				cedure
Ex-			MS	of
am-		Molecular	(M +	Ex-
ple	Structure	Formula	H)+	ample
7	23	C10H14N2O3S	243	3
8	24	C14H17N3O2S	292	2
9	25	C16H21N3O3S	336	2
10	26	C18H18N4O2S	355	4
11	27	C13H15N3O2S	278	4
12	28	C19H18F2N4O2S	405	4
13	29	C19H18F2N4O2S	405	4
14	30	C19H20N4O2S	369	4
15	31	C19H20N4O2S	369	4
16	32	C24H23N3O2S	419	4
17	33	C24H23N3O2S	419	4
18	34	C20H21N3O2S	368	4
19	35	C20H21N3O2S	368	4
20	36	C11H16N6OS	281	3
21	37	C14H14N2OS	259	2
22	38	C13H12N2OS	245	2
23	39	C17H20N2OS	301	2
24	40	C13H11ClN2OS	280	2
25	41	C13H14BrN3O2S	357	2
26	42	C13H13N3O2S	276	5
27	43	C13H14N2OS	247	3
28	44	C16H18N2OS	287	2
29	45	C16H20N2OS	289	3
30	46	C12H11N3OS	246	2
31	47	C12H18N6OS	295	3
32	48	C13H12N2OS	245	4
33	49	C12H11N3OS	246	4
34	50	C18H16F2N4O2S	391	4
35	51	C16H21N3O2S	320	4
36	52	C9H10N2O3S	227	2
37	53	C19H22N4O2S	371	3
38	54	C12H11N3OS	246	2
39	55	C12H11N3OS	246	2
40	56	C12H11N3OS	246	2
41	57	C12H11N3OS	246	2
42	58	C23H33N5O4S	477	4
43	59	C19H27N5O2S	391	4
44	60	C18H24N4O3S	377	4
45	61	C15H21N3O2S	308	3
46	62	C15H17N3O2S	304	5
47	63	C14H16FN3O2S	310	2
48	64	C17H19N5O2S	358	4
49	65	C18H25N5O2S	377	4
50	66	C22H23N3O5S	443	4
51	67	C20H28N4O3S	406	4
52	68	C14H12N4OS	285
53	69	C16H13N3O2S	312	2
54	70	C16H23N3OS	306	6
55	71	C20H23N3OS	354	6
56	72	C19H26N4O2S	376	4
57	73	C20H21N5O3S	412	4
58	74	C24H29N5O2S	453	4
59	75	C20H20N4O4S	413	4
60	76	C19H20N4O3S	385	4
61	77	C20H20Cl2N4O3S	468	4
62	78	C18H19N5O2S	370	4
63	79	C19H20N4O3S	385	4
64	80	C20H21N5O3S	412	4
65	81	C19H20N4O3S	385	4
66	82	C21H23N5O3S	427	4
67	83	C24H29N5O4S	485	4
68	84	C22H25N5O3S	441	4
69	85	C22H26N6O3S	456	4
70	86	C25H29N5O5S	513	4
71	87	C18H19N5O2S	370	4
72	88	C20H28N4O2S	390	4
73	89	C18H24N4O3S	377	4
74	90	C20H28N4O3S	406	4
75	91	C19H26N4O2S	376	4
76	92	C20H28N4O2S	390	4
77	93	C20H29N5O2S	405	4
78	94	C17H22N4O3S	363	4
79	95	C18H25N5O2S	377	4
80	96	C19H26N4O3S	392	4
81	97	C20H28N4O3S	406	4
82	98	C20H20N4O4S	413	4
83	99	C19H25N5O3S	405	4
84	100	C18H19N5O2S	370	4
85	101	C21H21N3O5S	428	4
86	102	C20H19N5O5S2	475	4
87	103	C19H26N4O3S	392	4
88	104	C19H19ClN4O3S	420	4
89	105	C24H30N6O2S	468	4
90	106	C20H22N4O2S	383	4
91	107	C17H18N6O4S	403	4
92	108	C21H20N6O4S	454	4
93	109	C19H26N4O3S	392	4
94	110	C22H30N4O4S	448	4
95	111	C20H26N4O4S	420	4
96	112	C19H24N4O4S	405	4
97	113	C19H17F3N4O2S	423	4
98	114	C19H26N4O2S	376	4
99	115	C20H19N5O2S	394	4
100	116	C18H18ClN5O2S	405	4
101	117	C17H25N3O3S	352	3
102	118	C15H19N3O2S	306	Scheme 2
103	119	C14H19N3O3S	310	Scheme 3
104	120	C18H20N4OS	341	6
105	121	C19H19F3N4O2S	425	3
106	122	C19H19N5O4S	414	4
107	123	C19H20N6O3S	413	4
108	124	C15H19N3O2S	306	Scheme 2
109	125	C15H19N3O3S	322	4
110	126	C20H28N4O2S	390	4
111	127	C26H34N4O3S	484	4
112	128	C19H19N3O2S	354	4
113	129	C18H17Cl2N5O2S	439	4
114	130	C19H16F2N4O2S	403	5
115	131	C14H17N3O3S	308	6
116	132	C14H18N4O2S	307	6
117	133	C15H21N3O2S	308	6
118	134	C14H16F3N3OS	332	6
119	135	C13H15N3O2S	278	4
120	136	C17H22N4O4S	379	4
121	137	C18H26N6O2S	392	4
122	138	C20H29N5O2S	405	4
123	139	C18H25N3O3S	364	6
124	140	C15H19N3O3S	322	6
125	141	C14H16N4OS	289	6
126	142	C17H19N5O2S	358	4
127	143	C18H19N3OS	326	6
128	144	C18H21N5O2S	372	4
129	145	C20H24N6O3S	430	4
130	146	C23H25N5O2S	437	4
131	147	C24H32N6O4S	502	4
132	148	C18H22N6O2S	387	4
133	149	C19H24N6O2S	402	4
134	150	C19H24N6O2S	402	4
135	151	C14H19N3O2S	294	6
136	152	C16H23N3O2S	322	6
137	153	C16H23N3O2S	322	6
138	154	C17H25N3O2S	336	6
139	155	C15H21N3O2S	308	6
140	156	C19H30N4OS	364	6
141	157	C18H22N6O3S	403	6
142	158	C17H23N3O3S	350	4
143	159	C20H23N3OS	354	6
144	160	C19H21N3OS	340	6
145	161	C18H18ClN3OS	361	6
146	162	C19H21N3O2S	356	6
147	163	C17H18N4OS	327	6
148	164	C19H27N5O2S	391	4
149	165	C16H17N5OS	328	6
150	166	C17H18N4OS	327	6
151	167	C18H18ClN3OS	361	6
152	168	C20H22N4O2S	383	4
153	169	C17H19N5O2S	358	4
154	170	C20H21N3O2S	368	4
155	171	C16H21N3O2S	320	4
156	172	C16H17N5OS	328	6
157	173	C19H18F2N4O2S	405	4
158	174	C19H20N4O3S	385	4
159	175	C19H21N3O2S	356	6
160	176	C19H21N3O2S	356	6
161	177	C21H20N4OS	377	6
162	178	C16H17N5OS	328	6
163	179	C23H29N5OS	425	6
164	180	C17H18N4O2S	343	6
165	181	C18H20N4OS	341	6
166	182	C17H18N4O2S	343	6
167	183	C26H32N6O3S	510	4
168	184	C20H22N4O2S	383	4
169	185	C25H31N5O3S	483	4
170	186	C26H32N4O3S	482	4
171	187	C16H17N5OS	328	6
172	188	C21H24N4O2S	398	4
173	189	C17H19N5OS	342	6
174	190	C15H19N3OS	290	6
175	191	C20H23N4O2S	385	4
176	192	C16H19N3O2S	318	5
177	193	C20H19N3O2S	366	5
178	194	C17H17N5O2S	356	5
179	195	C17H19N5O2S	358	4
180	196	C17H17Cl2N5O2S	427	4
181	197	C19H17N7O2S	408	4
182	198	C18H21N5O2S	372	4
183	199	C17H16N4OS	325	5
184	200	C17H18N4OS	327	6
185	201	C15H13N3S	268	6
186	202	C17H19N5O2S	358	6
187	203	C19H21N3O2S	356	6
188	204	C18H20N4OS	341	6
189	205	C18H20N4OS	341	6

Claims

1. A compound of the formula:

2. The compound as recited in claim 1, wherein R1═R2, COR3, or CONR2R3; R2=alkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl; R3=H, alkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl; 209, where n=0, 1, 2; m=1, 2, or , where i, j=0 or 1 but cannot both be 1, and Y=optionally substituted alkene, alkyne, or any two adjacent carbon atoms of a cycloalkyl ring; R4=alkyl with two or more carbon atoms, aryl, heteroaryl, or R9 with the proviso that when R1 is acetyl or propionyl and Y=alkene, then R4 cannot be nitrofuryl or 2-quinolinyl; R5, R6, R7, R8=independently H, or alkyl; 210where Z═O; R10, R11=independently H, alkyl or cycloalkyl.

3. The compound as recited in claim 1, wherein: R1═COR3; R3═H, alkyl, heteroaryl, arylalkyl, or heteroarylalkyl; 211, where n=0,1; m=1, or , where i, j=0 or 1 but cannot both be 1, and Y=an optionally substituted alkene; R4═R9; R5, R6, R7, R8=independently H, or alkyl. 212where Z═O; and R10, R11=independently H, alkyl or cycloalkyl.

4. The compound as recited in claim 1, wherein: R1═COR3; R3=alkyl, arylalkyl, heteroaryl, or heteroarylalkyl; 213where n=0,1; m=1; R5, R6, R7, R8=independently H, or alkyl; R4═R9; 214where Z═O; R10=alkyl or cycloalkyl, and R11═H.

5. The compound as recited in claim 1, wherein: R1═COR3; R3=alkyl, arylalkyl, heteroaryl, or heteroarylalkyl; 215, where i, j=0 or 1 but cannot both be 1, and Y=an optionally substituted alkene or alkyne; R4═R9; R5, R6, R7, R8=independently H, or alkyl; 216where Z═O; R10=alkyl or cycloalkyl,; and R11═H.

6. The compound as recited in claim 1, wherein: R1═COR3; R3=alkyl, arylalkyl, heteroaryl, or heteroarylalkyl; 217, where n=0,1; m=1, or , where i, j=0 or 1 but cannot both be 1, and Y=an optionally substituted alkene; R4=alkyl with two or more carbon atoms, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl with the proviso that when R1 is acetyl or propionyl and Y=alkene, then R4 cannot be nitrofuryl or 2-quinolinyl; R5, R6, R7, R8=independently H, or alkyl.

7. The compound as recited in claim 1, wherein: R1═R2; R2=alkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl; 218, where n=0,1; m=1, or , where i, j=0 or 1 but cannot both be 1, and Y=an optionally substituted alkene; R4═R9; R5, R6, R7, R8=independently H, or alkyl. 219where Z═O; and R10, R11=in dependently H, alkyl or cycloalkyl.

8. The compound as recited in claim 1, wherein: R1═R2; R2=alkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl; 220where n=0,1; m=1; R5, R6, R7, R8=independently H, or alkyl; R4═R9; 221where Z═O; R10=alkyl or cycloalkyl,; and R11═H.

9. The compound as recited in claim 1, wherein: R1═R2; R2=alkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl; 222, where i, j=0 or 1 but cannot both be 1, and Y=an optionally substituted alkene or alkyne; R4═R9; R5, R6, R7, R8=independently H, or alkyl; 223where Z═O; R10=alkyl or cycloalkyl,; and R11═H.

10. The compound as recited in claim 1, wherein: R1═R2; R2=alkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl; 224, where n=0,1; m=1, or, , where i, j=0 or 1 but cannot both be 1, and Y=an optionally substituted alkene; R4=alkyl with two or more carbon atoms, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl; R5, R6, R7, R8=independently H, or alkyl.

11. The compound as recited in claim 1, wherein: R1═CONR2R3; R2=alkyl, heteroaryl, arylalkyl, or heteroarylalkyl; R3═H, alkyl, heteroaryl, arylalkyl, or heteroarylalkyl; 225, where n=0,1; m=1, or , where i, j=0 or 1 but cannot both be 1, and Y=an optionally substituted alkene; R4═R9; R5, R6, R7, R8=independently H, or alkyl; 226where Z═O; and R10, R11=independently H, alkyl or cycloalkyl.

12. The compound as recited in claim 1, wherein: R1═CONR2R3; R2=alkyl, arylalkyl, heteroaryl, or heteroarylalkyl; R3=H, alkyl, heteroaryl, arylalkyl, or heteroarylalkyl; 227where n=0,1; m=1; R4═R9; R5, R6, R7, R8=independently H, or alkyl; 228where Z═O; R10=alkyl or cycloalkyl; and R11═H.

13. The compound as recited in claim 1, wherein: R1═CONR2R3; R2=alkyl, arylalkyl, heteroaryl, or heteroarylalkyl; R3=H, alkyl, heteroaryl, arylalkyl, or heteroarylalkyl; 229, where i, j=0 or 1 but cannot both be 1, and Y=an optionally substituted alkene or alkyne; R4═R9; R5, R6, R7, R8=independently H, or alkyl; 230where Z═O; R10=alkyl or cycloalkyl, and R11═H.

14. The compound as recited in claim 1, wherein: R1═CONR2R3; R2=alkyl, arylalkyl, heteroaryl, or heteroarylalkyl; R3═H, alkyl, heteroaryl, arylalkyl, or heteroarylalkyl; 231, where n=0,1; m=1, or, , where i, j=0 or 1 but cannot both be 1, and Y=an optionally substituted alkene; R4=alkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, heterocycloalkylalkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl; R5, R6, R7, R8=independently H, or alkyl.

15. A compound selected from the group consisting of: Ethyl 3-((E)-2-acetamido-thiazol-5-yl)-acrylate; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-acetamide; N-[5-(2-(5-t-Butyl-oxazol-2-yl)-ethyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-phenylacetamide; N-[5-(2-(5-t-Butyl-oxazol-2-yl)-ethynyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-aminopyridine; Ethyl 3-(2-acetamido-thiazol-5-yl)-propionate; N-[(Z)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-acetamide; [(E)-5-(2-(5-Isopropyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-carbamic acid t-butyl ester; N-[(E)-5-(2-(5-Isopropyl-oxazol -2-yl)-vinyl)-thiazol-2-yl]-2-pyridin-3-yl-acetamide; N-[(E)-5-(2-(5-Isopropyl-oxazol -2-yl)-vinyl)-thiazol-2-yl]-acetamide; 1-(2,6-Difluorophenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(2,6-Difluorophenyl)-3-[(Z)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; N-[(Z)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-pyridin-3-yl-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-pyridin-3-yl-acetamide; N-[(Z)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-naphthalen-2-yl-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-naphthalen-2-yl-acetamide; N-[(Z)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-phenylacetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-phenylacetamide; N-[5-(2-(2-Isopropyl-tetrazol-5-yl)-ethyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(3-Methylphenyl)-vinyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-Phenyl)-vinyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(4-t-Butyl-phenyl)-vinyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(3-Chlorophenyl)-vinyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-Bromo-2-(5-isopropyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-acetamide; N-[5-(2-(5-Isopropyl-oxazol-2-yl)-ethynyl)-thiazol-2-yl]-acetamide; N-[5-(2-Phenyl)-ethyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(3-Isopropylphenyl)-vinyl)-thiazol-2-yl]-acetamide; N-[5-(2-(3-Isopropylphenyl)-ethyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(3-Pyridinyl)-vinyl)-thiazol-2-yl]-acetamide; N-[5-(2-(2-t-Butyl-tetrazol-5-yl)-ethyl)-thiazol-2-yl]-acetamide; N-[(Z)-5-(2-Phenyl)-vinyl)-thiazol-2-yl]-acetamide; N-[(Z)-5-(2-(3-Pyridinyl)-vinyl)-thiazol-2-yl]-acetamide; 1-(2,6-Difluorophenyl)-3-[(E)-5-(2-(5-isobutyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-isobutyramide; Ethyl 3-((Z)-2-acetamido-thiazol-5-yl)-acrylate; N-[5-(2-(5-t-Butyl-oxazol-2-yl)-ethyl)-thiazol-2-yl]-2-pyridin-3-yl-acetamide; N-[(E)-5-(2-(4-Pyridinyl)-vinyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(2-Pyridinyl)-vinyl)-thiazol-2-yl]-acetamide; N-[(Z)-5-(2-(4-Pyridinyl)-vinyl)-thiazol-2-yl]-acetamide; N-[(Z)-5-(2-(2-Pyridinyl)-vinyl)-thiazol-2-yl]-acetamide; 4-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-ylcarbamoylmethyl]-piperazine-1-carboxylic acid t-butyl ester; 1-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-ylcarbamoylmethyl]-4-methylpiperazine; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-morpholin-4-yl-acetamide; N-[5-(3-(5-t-Butyl-oxazol-2-yl)-propyl)-thiazol-2-yl]-acetamide; N-[5-(3-(5-t-Butyl-oxazol-2-yl)-propynyl)-thiazol-2-yl]-acetamide; N-[(Z)-5-(2-Fluoro-2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(3H-imidazol-4-yl)-acetamide; )-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-piperazin-1-yl-acetamide; Methyl 4-N-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-carbamoylmethoxy-benzoate; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(1-methyl-piperidin-4-yloxy)-acetamide; N-[5-(5-Phenyl-3H-imidazol-4-yl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(5-phenyloxazol-2-yl)-vinyl)-thiazol-2-yl]-acetamide; Isobutyl-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-amine; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-phenethylamine; 1-Cyclohexyl-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(4-Carbamoylphenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(4-Piperidinylphenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(Benzo[1,3]dioxol-5-yl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(2-Hydroxyphenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(2,6-Dichloro-4-methoxyphenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(2-Pyridinyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(3-Hydroxyphenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(3-Carbamoylphenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(4-Hydroxyphenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(3-Carbamoyl-4-methylphenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 2-Dimethylamino-ethyl 4-{3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol -2-yl]-ureido}-benzoate; 1-[4-(N,N-Dimethylcarbamoyl)phenyl]-3-[(E)-5-(2-(5-butyl-oxazol-2-yl)-vinyl)-thiazol -2-yl]-urea; 1-(2-Morpholinylpyridin-5-yl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol -2-yl]-urea; Methyl 5-{3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-ureido}-2-N-morpholino-benzoate; 1-(3-Pyridinyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(2-Methylcyclohexyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 4-Hydroxy-piperidine-1-carboxylic acid [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol -2-yl]-amide; 2-(2-Hydroxyethyl)-piperidine-1-carboxylic acid [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-amide; 2-Methyl-piperidine-1-carboxylic acid [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol -2-yl]-amide; 1-Cyclohexyl-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-1-methyl-urea; 3-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-1-methyl-1-(1-methyl-piperidin-4-yl)-urea; 3-Hydroxy-pyrrolidine-1-carboxylic acid [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol -2-yl]-amide; 4-Methyl-piperazine-1-carboxylic acid [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-amide 4-Hydroxymethyl-piperidine-1-carboxylic acid [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-amide; 4-(2-Hydroxyethyl)-piperidine-1-carboxylic acid [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl )-thiazol-2-yl]-amide; 1-[4-carboxyphenyl]-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; Piperidine-1,4-dicarboxylic acid 4-amide 1-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-amide; 1-(4-Pyridinyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 4-N-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-carbamoylmethoxy-benzoic acid; 6-{3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-ureido}-1,1-dioxo-1,2,3a,7a-tetrahydro-1λ6-benzo[d]isothiazol-3-one; 3-Hydroxymethyl-piperidine-1-carboxylic acid [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-amide; 1-(2-Chloro-4-hydroxyphenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-[4-(4-Methyl-piperazin-1-yl)phenyl]-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 3-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-1-methyl-1-phenyl-urea; 1-(2,6-Dihydroxy-pyrimidin-4-yl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-3-(2,3-dihydro-phthalazine-1,4-dion-6-yl)-urea; 1-[(1S,2S)-2-Hydroxycyclohexyl]-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; (1R,2S)-2-{3-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-ureido}-cyclohexanecarboxylic acid ethyl ester; (1R,2S)-2-{3-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-ureido}-cyclohexanecarboxylic acid; 1-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-2-fluoro-vinyl)-thiazol-2-yl]carbamoyl-piperidine-4-carboxylic acid; 1-(2,6-Difluorophenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-2-fluoro-vinyl)-thiazol-2-yl]-urea; 2-Piperidin-4-yl-N-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-acetamide; 1-(4-cyanophenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(2-Chloro-pyridin-5-yl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; [5-(2-(5-t-Butyl-oxazol-2-yl)-ethyl)-thiazol-2-yl]-carbamic acid t-butyl ester; N-[5-((S)-2-(5-t-Butyl-oxazol-2-yl)-cyclopropyl)-thiazol-2-yl]-acetamide; N-[5-(2-(5-t-Butyl-oxazol-2-yl)-2-hydroxyethyl)-thiazol-2-yl]-acetamide; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-pyridin-3-ylmethyl-amine; 1-(2,6-Difluorophenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-2-fluoro-ethyl)-thiazol-2-yl]-urea; 1-(5-Carboxy-pyridin-2-yl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(5-Carbamoyl-pyridin-2-yl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; N-[5-((R)-2-(5-t-Butyl-oxazol-2-yl)-cyclopropyl)-thiazol-2-yl]-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-(3-hydroxy)-propionamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(N-methylpiperidin-4-yl)-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-((4-(2-diethylamino)ethoxy)phenyl)-acetamide; N-[(E)-5-(2-(5-Isopropyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-phenyl-acetamide; 1-(3,5-Dichloro-pyridin-4-yl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(2,6-Difluorophenyl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-ethynyl)-thiazol-2-yl]-urea; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-ylamino)-acetic acid; 2-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-ylamino]-acetamide; 3-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-ylamino]-propan-1-ol; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(2,2,2-trifluoroethyl)-amine; N-[(E)-5-(3-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-formamide; (3R,4R)-3,4-Dihydroxy-pyrrolidine-1-carboxylic acid [(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-amide; 1-(4-methylpiperizin-1-yl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; 1-(2,6-Dimethylpiperidin-1-yl)-3-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-urea; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-ylamino]-acetic acid, t-butyl ester; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-ylamino]-acetic acid, methyl ester; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(cyanomethyl)-amine; N-[(E)-5-(3-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(imidazol-1-yl)-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-aniline; N-[(E)-5-(3-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(1-methyl-imidazol-4-yl)-acetamide; N-[(E)-5-(3-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-3[(imidazol-4-yl)]-2-(N′-acetylamino)-propionamide; N-[(E)-5-(3-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-[3-(1-methyl-benzimidazol-2-yl)]-propionamide; (S)-N-[(E)-5-(3-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-3[(1-methyl-imidazol-4-yl)]-2-(N-t-butoxyacetylamino)-propionamide; (S)-N-[(E)-5-(3-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-3[(1-methyl-imidazol-4-yl)]-2-(N-t-butoxyacetylamino)-propionamide; (S)-N-[(E)-5-(3-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-3-(imidazol-4-yl)-2-amino-propionamide; (S)-N-[(E)-5-(3-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-3-(1-methyl-imidazol-5-yl)-2-amino-propionamide; (S)-N-[(E)-5-(3-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-3-(1-methyl-imidazol-4-yl)-2-amino-propionamide; 2-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-ylamino]-ethanol; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(4-hydroxy-butyl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-(3-methoxypropyl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(3-hydroxy-3-methyl-butyl)-amine; (Z)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(3-hydroxy-propyl)-amine; N′-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-N,N-dimethyl-pentane-1,5-diamine; 6-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-ylamino]-4-propoxy-[1,3,5]triazin-2-ol; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-carbamic acid t-butyl ester; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(2,6-dimethylphenyl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(2-methylphenyl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(4-chlorophenyl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(4-methoxyphenyl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(pyridyl-3-yl)-amine; 3-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-1-(1-methyl-piperidin-4-yl)-urea; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(pyrazine-2-yl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(pyridyl-4-yl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(3-chloro-phenyl)-amine; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(2-methylpyridin-3-yl)-acetamide; N-[(Z)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(imidazol-1-yl)-acetamide; N-[(Z)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-phenylacetamide; N-[(Z)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-isobutyramide; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(pyrimidin-2-yl)-amine; 1-[(Z)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-3-(2,6-difluorophenyl)-urea; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(1-oxy-pyridin-3-yl)-acetamide; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(2-methoxyphenyl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(3-methoxyphenyl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(isoquinolin-3-yl)-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl-(pyrimidin-4-yl)-amine; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-[4-(4-methyl-piperazin-1-yl)-phenyl]-amine; (E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-(1-oxy-pyridin-2-yl)-amine; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-benzene-1,3-diamine; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-(1-oxy-pyridin-4-yl)-amine; 3-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-1-(4-(N-pyrrolidin-1-ylethyl-carbamoyl)phenyl)-urea; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(pyridin-3-yl)-propionamide; 3-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-1-[4-(pyrrolidin-1-ylethoxy)-phenyl]-urea; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-[4-(pyrrolidin-1 -ylethoxy)-phenyl]-acetamide; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-pyridazin-3-yl-amine; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(2-methyl-pyridin-3-yl)-propionamide; N-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-pyridine-2,6-diamine; Cyclopropyl-[(E)-5-(2-(5-t-butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-amine; 3-(N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-carbamoyl-methyl)-1-methyl-pyridinium iodide; N-[5-(5-t-Butyl-oxazol-2-yl)-ethynyl)-thiazol-2-yl]-isobutyramide; [5-(5-t-Butyl-oxazol-2-yl)-ethynyl)-thiazol-2-yl]-2-phenylacetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-imidazol-1-yl-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-pyrazol-1-yl-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(4,5-dichloro-imidazol-1-yl)-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(4,5-dicyano-imidazol-1-yl)-acetamide; N-[(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-2-(2-methyl-imidazol-1-yl)-acetamide; [5-(5-t-Butyl-oxazol-2-yl)-ethynyl)-thiazol-2-yl]-(pyridin-2-yl)-amine; [(Z)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-(pyridin-2-yl)-amine; (5-Benzyl-thiazol-2-yl)-pyridin-2-yl-amine; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-(6-methoxy-pyrimidin-4-yl)-amine; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-(3-hydroxymethyl-phenyl)-amine; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-(4-methyl-pyridin-2-yl)-amine; [(E)-5-(2-(5-t-Butyl-oxazol-2-yl)-vinyl)-thiazol-2-yl]-(6-methyl-pyridin-2-yl)-amine.

16. A pharmaceutical composition comprising a compound of claim 1 and a pharmaceutically acceptable carrier.

17. A pharmaceutical composition comprising a compound of claim 1 in combination with pharmaceutically acceptable carrier and an anti-cancer agent formulated as a fixed dose.

18. A method of inhibiting protein kinases which comprises administering to a mammalian specie in need thereof an effective protein kinase inhibiting amount of a compound of claim 1.

19. A method of inhibiting cyclin dependent kinases which comprises administering to a mammalian specie in need thereof an effective cyclin dependent kinase inhibiting amount of a compound of claim 1.

20. A method of inhibiting cdc2 (cdk1) which comprises administering to a mammalian specie in need thereof an effective cdc2 inhibiting amount of a compound of claim 1.

21. A method of inhibiting cdk2 which comprises administering to a mammalian specie in need thereof an effective cdk2 inhibiting amount of a compound of claim 1.

22. A method of inhibiting cdk3 which comprises administering to a mammalian specie in need thereof an effective cdk3 inhibiting amount of a compound of claim 1.

23. A method of inhibiting cdk4 which comprises administering to a mammalian specie in need thereof an effective cdk4 inhibiting amount of a compound of claim 1.

24. A method of inhibiting cdk5 which comprises administering to a mammalian specie in need thereof an effective cdk5 inhibiting amount of a compound of claim 1.

25. A method of inhibiting cdk6 which comprises administering to a mammalian specie in need thereof an effective cdk6 inhibiting amount of a compound of claim 1.

26. A method of inhibiting cdk7 which comprises administering to a mammalian specie in need thereof an effective cdk7 inhibiting amount of a compound of claim 1.

27. A method of inhibiting cdk8 which comprises administering to a mammalian specie in need thereof an effective cdk8 inhibiting amount of a compound of claim 1.

28. A method for treating proliferative diseases comprising administering to a mammalian specie in need thereof a therapeutically effective amount of a composition of claim 17.

29. A method for treating cancer comprising administering to a mammalian specie in need thereof a therapeutically effective amount of a composition of claim 17.

30. A method for treating inflammation, inflamatory bowel disease, or transplantation rejection, comprising administering to a mammalian specie in need thereof a therapeutically effective amount of a composition of claim 17.

31. A method for treating arthritis comprising administering to a mammalian specie in need thereof a therapeutically effective amount of a composition of claim 17.

32. A method for treating proliferative diseases comprising administering to a mammalian specie in need thereof a therapeutically effective amount of a composition of claim 18.

33. A method for treating cancer comprising administering to a mammalian specie in need thereof a therapeutically effective amount of a composition of claim 18.

34. A method for treating inflammation, inflammatory bowel disease, or transplantation rejection, comprising administering to a mammalian specie in need thereof a therapeutically effective amount of a composition of claim 18.

35. A method for treating arthritis comprising administering to a mammalian specie in need thereof a therapeutically effective amount of a composition of claim 18.