Claims
- 1. A method of producing a ketocarotenoid in a host cell, the method comprisinginserting into the host cell a vector comprising a heterologous nucleic acid sequence which encodes for a protein having ketolase enzyme activity and has the nucleic acid sequence of SEQ ID NO: 1 or 3, wherein the heterologous nucleic acid sequence is operably linked to a promoter; and expressing the heterologous nucleic acid sequence, thereby producing the ketocarotenoid.
- 2. The method of claim 1, wherein the host cell is selected from the group consisting of a bacterial cell, an algal cell and a plant cell.
- 3. A method of producing a ketocarotenoid in a host cell, the method comprisinginserting into the host cell a vector comprising a heterologous nucleic acid sequence which encodes for a protein having ketolase enzyme activity and has a sequence which encodes the amino acid sequence of SEQ ID NO: 2 or 4, wherein the heterologous nucleic acid sequence is operably linked to a promoter; and expressing the heterologous nucleic acid sequence, thereby producing the ketocarotenoid.
- 4. The method of claim 3, wherein the host cell is selected from the group consisting of a bacterial cell, an algal cell and a plant cell.
- 5. A method of modifying the production of carotenoids in a host cell, relative to an untransformed host cell, the method comprisinginserting into a host cell which already produces carotenoids a vector comprising a heterologous nucleic acid sequence which encodes for a protein having ketolase enzyme activity and has the nucleic acid sequence of SEQ ID NO: 1 or 3, wherein the heterologous nucleic acid sequence is operably linked to a promoter; and expressing the heterologous nucleic acid sequence in the host cell to modify the production of the carotenoids in the host cell, relative to an untransformed host cell.
- 6. The method of claim 5, wherein the host cell is selected from the group consisting of a bacterial cell, an algal cell and a plant cell.
- 7. A method of modifying the production of carotenoids in a host cell, relative to an untransformed host cell, the method comprisinginserting into a host cell which already produces carotenoids a vector comprising a heterologous nucleic acid sequence which encodes for a protein having ketolase enzyme activity and has a sequence which encodes the amino acid sequence of SEQ ID NO: 2 or 4, wherein the heterologous nucleic acid sequence is operably linked to a promoter; and expressing the heterologous nucleic acid sequence in the host cell to modify the production of the carotenoids in the host cell, relative to an untransformed host cell.
- 8. The method of claim 7, wherein the host cell is selected from the group consisting of a bacterial cell, an algal cell and a plant cell.
- 9. A purified nucleic acid sequence which encodes for a protein having ketolase enzyme activity and has the nucleic acid sequence of SEQ ID NO: 1.
- 10. A purified nucleic acid sequence which encodes for a protein having ketolase enzyme activity and has the nucleic acid sequence of SEQ ID NO: 3.
- 11. A purified nucleic acid sequence which encodes for a protein having ketolase enzyme activity and has a sequence which encodes the amino acid sequence of SEQ ID NO: 2.
- 12. A purified nucleic acid sequence which encodes for a protein having ketolase enzyme activity and has a sequence which encodes the amino acid sequence of SEQ ID NO: 4.
- 13. A vector which comprises the nucleic acid sequence of any one of claims 9-12, wherein the nucleic acid sequence is operably linked to a promoter.
- 14. A host cell which is transformed with the vector of claim 13.
- 15. The host cell of claim 14, wherein the host cell is selected from the group consisting of a bacterial cell, an algal cell and a plant cell.
- 16. The host cell of claim 14, wherein the host cell is a photosynthetic cell.
- 17. The host cell of claim 14, wherein the host cell contains a ketocarotenoid.
- 18. The host cell of claim 14, wherein the host cell contains modified levels of carotenoids, relative to an untransformed host cell.
- 19. A purified ketolase enzyme comprising the amino acid sequence of SEQ ID NO: 2.
- 20. A purified ketolase enzyme comprising the amino acid sequence of SEQ ID NO: 4.
Parent Case Info
This application is a 371 of PCT//US99/10455 filed May. 21, 1999, which claims the benefit of U.S. provisional application No. 60/086,460, filed May 22, 1998.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/US99/10455 |
|
WO |
00 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO99/61652 |
12/2/1999 |
WO |
A |
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Number |
Name |
Date |
Kind |
5453565 |
Mawson |
Sep 1995 |
A |
5744341 |
Cunningham, Jr. et al. |
Apr 1998 |
A |
5811273 |
Misawa et al. |
Sep 1998 |
A |
5910433 |
Kajwara et al. |
Jun 1999 |
A |
Provisional Applications (1)
|
Number |
Date |
Country |
|
60/086460 |
May 1998 |
US |