Claims
- 1. A method for carrying out an immunological analysis which comprises the steps of: providing a reagent carrier comprising a carrier fleece consisting essentially of
- a) from 5 to 60% by weight of cellulose-containing fibres,
- b) from 40 to 95% by weight of polymer fibres selected from the group consisting of polyester, polyamide and mixture thereof, and
- c) an organic binding agent which has a hydroxyl or an ester group or a combination thereof in an amount of from 5 to 30% by weight of the fibre components,
- said fleece being impregnated with an immunologically active agent selected from the group consisting of haptens, antigens, antibodies, antibody fragments, and conjugates thereof with labelling substances and/or synthetic peptides, said agent being easily and substantially completely elutable into a solution of a sample containing an immunologically active substance to be analyzed; introducing said reagent carrier into a composition comprising a solvent and said sample, said solvent being a solvent for the immunologically active agent, whereby the immunologically active agent is eluted into the solution; and determining the presence or amount of the immunologically active substance which reacts with the immunologically active agent.
- 2. A method according to claim 1 wherein, in the carrier fleece, 60 to 40% by weight of the fibres are cellulose-containing fibres, 40 to 60% by weight of the fibres are polyamide, and the organic binding agent is polyvinyl alcohol.
- 3. A method according to claim 1 wherein, in the carrier fleece, 5 to 40% by weight of the fibres are cellulose-containing fibres, 60 to 95% by weight of the fibres are polyester, and the organic binding agent is polyvinyl alcohol.
- 4. A method according to claim 1 wherein the cellulose-containing fibres are selected from the group consisting of regenerated cellulose, linters and cellulose pulp.
- 5. The method of claim 4 wherein the cellulose-containing fibres consist of a mixture of regenerated cellulose and linters.
- 6. The method of claim 5 wherein the ratio of regenerated cellulose to linters is from 5:1 to 1:1.
- 7. A method according to claim 1 wherein, in the carrier fleece, the polyester fibres have a weight of about 1.17 g/cm.sup.3, a length of cut of 3 to 6 mm, and a fibre fineness of 1.7 to 3.3 dtex.
- 8. A method according to claim 1 wherein, in the carrier fleece, the polyamide fibres have a specific weight of 1.14 to 1.15 g/cm.sup.3, a length of cut of 4 to 6 mm, and a fibre fineness of 2.2 dtex.
- 9. A method according to claim 2 in which the immunologically active agent impregnated into the carrier fleece a beta-galactosidase conjugate.
- 10. A method according to claim 2 in which the reagent carrier additionally comprises binding components selected from the group consisting of biotin, avidin, streptavidin, protein A, IgG, concanavalin A and mannose.
Priority Claims (1)
Number |
Date |
Country |
Kind |
3802366 |
Jan 1988 |
DEX |
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Parent Case Info
This is a continuation of application Ser. No. 296,429 filed on Jan. 12, 1989 now abandoned.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
4803171 |
Baier et al. |
Feb 1989 |
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4820633 |
Herrmann |
Apr 1989 |
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4820644 |
Schafer et al. |
Apr 1989 |
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Non-Patent Literature Citations (2)
Entry |
Kazu et al., Abstract Bulletin, vol. 58, No. 1, Jul. 1987, p. 137, Abstract No. 1069. |
Shinoki et al., Abstract Bulletin, vol 58, No. 3, Sep. 1987, p. 410, Abstract No. 3676. |
Continuations (1)
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Number |
Date |
Country |
Parent |
296429 |
Jan 1989 |
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