Patent Grant
10989723
None.
One barrier to the broad adoption of rapid DNA-based human identification is the consumable cost. A low-cost cartridge using small amounts of reagents would reduce this barrier. Previous approaches have focused on automation and manufacturing process improvement to reduce the cost to make a given cartridge.
Versions of systems including sample cartridges and fluidic systems for sample extraction and analysis are described in, for example, U.S. Pat. No. 6,190,616 (Jovanovich et al.); U.S. Pat. No. 6,551,839 (Jovanovich et al.); U.S. Pat. No. 6,870,185 (Jovanovich et al.); U.S. Pat. No. 7,244,961 (Jovanovich et al.); U.S. Pat. No. 7,445,926 (Mathies et al.); U.S. Pat. No. 7,799,553 (Mathies et al.); U.S. Pat. No. 8,173,417 (Tan et al.); U.S. Pat. No. 8,206,974 (Tan et al.); U.S. Pat. No. 8,394,642 (Jovanovich et al.); U.S. Pat. No. 8,425,861 (Selden et al.); U.S. Pat. No. 8,431,340 (Jovanovich et al.); U.S. Pat. No. 8,720,036 (Selden et al.) and U.S. Pat. No. 8,858,770 (Tan et al.); US patent applications 2009/0178934 (Jarvius); 2009/0253181; 2011/0039303 (Jovanovich et al.); 2011/0126911 (Kobrin et al.); 2011/0220502 (Selden et al.); 2012/0181460 (Eberhart et al.); 2013/0139895 (Vangbo) and 2013/0115607 (Nielsen et al.); and International Patent Application WO/US2013/130910.
Fluidic devices are provided, for example in the form of a cartridge, for sample extraction and analyte reaction and analysis.
Provided herein is a cartridge comprising one or more fluidic circuits that each comprise at least one diaphragm valve; wherein the cartridge comprises: (a) a body comprising (i) a surface comprising a valve seat in fluidic communication with a valve inlet and a valve outlet and (ii) at least one port in fluidic communication with the fluidic circuit; and (b) a layer of deformable material covering the valve seat and the at least one port, wherein a portion of the layer of deformable material functions as a diaphragm which, in combination with the valve seat, forms a diaphragm valve, and wherein the diaphragm, when actuated (for example, by being moved into contact with the valve seat or by being moved out of contact with the valve seat), regulates fluid flow across the diaphragm valve, and comprising at least one conduit through the layer of deformable material, each conduit communicating with a port; and wherein the cartridge is configured to engage a cartridge interface, putting the deformable material in direct contact with a surface of cartridge interface, putting the diaphragm in communication with a source of positive and/or negative pressure that actuates the diaphragm (e.g., pneumatically or mechanically) and putting the at least one port in communication with a fluid or pneumatic line through the conduit, wherein the layer of deformable material optionally functions as a gasket sealing the at least one port against leakage. In one embodiment the fluidic circuit further comprises a reaction chamber formed in the body, optionally covered with a film of heat conductive material (e.g., a metal film). In another embodiment the fluidic circuit further comprises a chamber configured to receive a sample comprising a biological material, said chamber optionally comprising a close tab. An example of such an instrument and cartridge is shown in
Also provided herein is an instrument comprising: (a) at least one cartridge interface comprising: (i) an engagement unit configured to receive a removably insertable cartridge and to engage a received cartridge with a manifold assembly and, optionally, a thermal regulator, wherein the cartridge comprises at least one fluidic circuit comprising at least one fluid channel, at least one exit port; and at least one diaphragm valve comprising a valve seat configured to regulate fluid flow in the at least one fluidic circuit; and a layer of deformable material covering the valve seat and the at least one port, wherein the deformable material functions as a diaphragm in the diaphragm valve and comprises at least one conduit through the layer, each conduit communicating with a port; and (ii) a manifold assembly comprising an engagement surface configured to directly contact the deformable layer of a received cartridge and having a plurality of transmission channels communicating with ports on the engagement surface; wherein engaging a cartridge with the manifold assembly: (A) puts the diaphragm in communication with a first port on the engagement surface configured to transmit positive or negative pressure to the diaphragm, and (B) puts the exit ports in communication with second ports on the engagement surface configured to transmit fluid into or out of a fluidic circuit through the transmission channels; and (iii) a thermal regulator which, when engaged with a received cartridge, puts a heat pump (e.g., a thermoelectric heater, e.g., a Peltier device) in thermal contact with a thermal cycling chamber in the cartridge or a heating element in thermal contact with a chamber in the cartridge. In one embodiment the instrument of further comprises any of: (b) a pneumatic pressure source configured to deliver positive or negative pneumatic pressure to a transmission channel of the manifold assembly; (c) a pump configured to move liquid into or out of a transmission channel of the manifold assembly; (d) a source of reagents in fluid communication with a transmission channel of the manifold assembly; (e) an analysis module configured to perform at least one analysis (e.g., electrophoresis) on a fluid received from the cartridge; and (f) a control module comprising executable code that, when executed, controls operation of the instrument.
Also provided herein is a cartridge comprising a first layer and a deformable layer: (a) wherein the first layer comprises: (i) a first side contacting the deformable layer, wherein the first side comprises a plurality of fluidic circuits, each fluidic circuit comprising at least one fluidic channel; and (ii) a second side comprising at least one fluidic distribution channel, which fluidic distribution channel is covered by a cover layer; (iii) a plurality of vias in the first layer, each via configured to put the fluidic distribution channel in communication with a fluidic channel; and (iv) optionally, at least one via in the first layer configured to put the fluidic distribution channel in communication with a port on a side of the first layer configured to engage a source of fluid; and (b) optionally comprising an actuation layer comprising at least one actuation circuit configured to actuate a diaphragm against a valve seat in the first side. An example of such a cartridge is shown in
Also provided herein is a cartridge comprising a first layer, a second layer and a deformable layer sandwiched there between: (a) wherein the first layer comprises: (i) a first side contacting the deformable layer, wherein the first side comprises a plurality of fluidic circuits, each fluidic circuit comprising at least one fluidic channel and at least one valve seat; (ii) a second side comprising at least one pneumatic distribution channel, which pneumatic distribution channel is optionally covered by a cover layer; (iii) at least one via in the first layer configured to put a pneumatic distribution channel in communication with the deformable layer; and (iv) optionally, at least one via in the first layer configured to put a pneumatic distribution channel in communication with a port on a side of the first layer configured to engage in a source of pneumatic pressure; (b) wherein portions of the deformable layer, in combination with valve seats in the first layer form diaphragm valves; and (c) wherein the actuation layer comprises at least one actuation circuit comprising at least one branch channel, wherein each branch channel is configured to actuate a diaphragm against a valve seat in a different fluidic circuit and further comprises a valve seat; and wherein positive or negative pressure applied to a pneumatic distribution channel transmits pressure through the via to actuate a diaphragm portion of the deformable layer into or out of contact with the valve seat in the branched channel, wherein closing the control valve inhibits actuation the diaphragm valve in the fluidic circuit. An example of such a configuration is shown in
Also provided herein is a cartridge comprising: (a) a body comprising a polymer and comprising at least one functional feature on a surface of the body and configured to transmit fluid (e.g., a port, a via, a fluid channel, a chamber, a valve inlet and valve outlet and/or a valve seat); and (b) a layer of deformable material thermally bonded to the body and covering the functional feature (optionally, wherein at least one portion of the layer comprises a permanent deformation), and wherein application of positive or negative pressure to at least one portion of the layer actuates the deformable material into or out of contact with a functional feature on the surface of the body; and wherein the cartridge is configured to engage a cartridge interface configured to supply positive or negative pressure to the at least one portion of the layer. In one embodiment the cartridge further comprises a fluid-filled chamber in the body, wherein the chamber has an opening sealed closed with a heat seal material. In another embodiment the deformable material comprises a heat seal material. In another embodiment the deformable material comprises a material selected from polypropylene, polyethylene, polystyrene, cycloolefin co-polymer (COC), mylar, polyacetate and a metal. An example of valves in such a cartridge is shown in
Also provided herein is a device comprising the aforementioned cartridge and a ram configured to actuate a diaphragm of a diaphragm valve on the body.
Also provided herein is a device comprising a cartridge and a clamping device: (a) wherein the cartridge comprises: (i) a body comprising: (A) at least one fluidic circuit comprising: (I) at least one functional feature on a surface of the body and configured to transmit fluid (e.g., a port, a fluid channel, a chamber, a valve inlet and valve outlet and/or a valve seat), wherein the functional feature optionally comprises a ridge on the surface of the body; and (II) at least one compartment containing a liquid, wherein the compartment communicates with the functional feature through one or more vias in the body; and (ii) a layer of deformable material covering the functional feature; and (b) wherein the clamping device, when engaged with the cartridge, applies sufficient pressure to the deformable material to deform the deformable material against the cartridge body and seal against movement of liquid from the compartment and through the at least one functional feature; and wherein removing the clamping device releases pressure to the deformable material, allowing the seal to open (e.g., through an elastic response of the deformable material or through application of positive or negative pressure against the seal). In one embodiment the clamping device comprises a mechanical clamp or a vacuum seal. An example of such a cartridge is shown in
Also provided herein is a cartridge comprising: (a) a fluidics layer comprising a surface having at least one diaphragm valve comprising a valve seat (e.g., a recessed valve seat); (b) a deformable layer mated to the surface, wherein a portion of the deformable layer functions as a diaphragm which, when actuated, is configured to move into or out of contact with the valve seat; and wherein the portion of the deformable layer functioning as a diaphragm comprises a boss positioned on a side of the deformable layer opposite of a side that contacts the valve seat; and (c) optionally comprising: a rigid substrate mated with the deformable layer and comprising apertures exposing the bosses and configured to receive a ram that contacts the boss and actuates the diaphragm, e.g., by application of mechanical pressure; or configured to engage an interface comprising apertures exposing the bosses and configured to receive a ram that contacts the boss and actuates the diaphragm, e.g., by application of mechanical pressure. An example of such a cartridge is shown in
Also provided herein is a cartridge comprising: (a) a base comprising: (I) a central barrel comprising a pump chamber and movable syringe, (II) a base floor comprising a port station comprising a floor port; and (III) a channel fluidically connecting the barrel chamber to the port in the floor; and (b) a turret configured to revolve around the central barrel and comprising a plurality of turret chambers, each turret chamber comprising a turret chamber aperture in a chamber floor of the turret chamber and facing the base floor, wherein positioning a turret chamber at the port station puts the turret chamber aperture in fluid communication with the barrel chamber through the floor port, and wherein the floor closes a turret chamber aperture when the turret chamber is positioned at at least one position other than the port station; and wherein at least one turret chamber further comprises a channel communicating between the floor port and an exit port. An example of such a cartridge is shown in
Also provided herein is a instrument comprising a cartridge interface and a removable cartridge engaged therewith: (a) wherein the interface comprises a base and one or more hollow bore pins for delivering fluid to a port in the cartridge, wherein the pin is biased against the base by the cartridge and protrudes through an aperture in the base; and wherein the pin comprises a home lead-in configured to put the pin in a home position after the bias is released by removal of the cartridge; and a tapered end configured to mate with a guide in the cartridge; and (b) wherein the cartridge comprises a fluidic circuit comprising a port and a pin guide communicating with the port, wherein the pin guide comprises a mating cone configured to mate with a tapered end of the pin and a pin guide lead-in configured to guide the pin into the mating cone when the cartridge exerts a bias against the tapered end of the pin during engagement with the interface. An example of such a combination is shown in
Also provided herein is a cartridge comprising: (a) a body comprising at least one puncturing element, at least one fluidic channel and at least one flange; and (b) a reagent reservoir comprising at least one fluidically isolated, fluid filled compartment; a breakable seal in a wall of the compartment and a breakable tab attached to an outside wall of the compartment; wherein the cartridge is configured such that when the reagent reservoir is engaged with the body: (i) the puncturing elements punctures the breakable seal, putting the compartment in fluidic communication with the channel; and (ii) the flange exerts a force against the breakable tab, breaking the tab and forming a vent in the compartment. An example of such an embodiment is shown in
Also provided herein is a fluidic device comprising one or more diaphragm valves, each diaphragm valve configured to regulate fluid flow in a fluidic channel, wherein the fluidic device comprises a fluidics layer, an actuation element and a deformable membrane, wherein each diaphragm valve comprises: a) a diaphragm comprised in the deformable membrane; b) a valve seat comprised in the fluidics layer and recessed from a surface of the fluidics layer that contacts the deformable membrane so that the diaphragm does not close the diaphragm valve unless positive pressure is exerted on the diaphragm; and c) a valve inlet and a valve outlet comprised in the fluidics layer and in fluid communication with a fluidic channel; and d) a ram configured to actuate the diaphragm; wherein the deformable membrane comprises a plastic material adhered to the fluidics layer through a heat activated adhesive, thermal fusion, chemical bonding or a pressure sensitive adhesive, and wherein the deformable membrane is configured such that pressure on the deformable membrane presses the membrane against the valve seat, thereby closing the valve and wherein the valve can be opened by releasing pressure of the ram against the deformable membrane or by pushing liquid through the valve. In one embodiment the deformable membrane is not an elastomeric material, e.g., is not PDMS. In another embodiment the deformable membrane comprises a laminate comprising the plastic material and a deformable, space-filling material, wherein pressure against the space-filling material causes the space filling material to fill the valve chamber sufficiently so that of the plastic material closes the valve. In another embodiment the deformable material has a durometer value of between 10 to 80 Shore D. In another embodiment the deformable material has a thickness sufficient such that the mechanical pressure is applied the deformable material sufficiently fills the valve chamber to form a seal between the plastic material in a valve seat to close the valve. In another embodiment the deformable, space-filling material comprises a solid foam. In another embodiment the deformable material is attached to the plastic material through an adhesive. In another embodiment the deformable material is pressed into contact with the plastic layer through an interface device. In another embodiment the fluidics layer comprises a polymer, e.g. a thermoplastic.
Also provided herein is a fluidic device comprising one or more diaphragm valves, each diaphragm valve configured to regulate fluid flow one or more fluidic channels, wherein the fluidic device comprises a fluidics layer, an actuation element and a deformable membrane, wherein each diaphragm valve comprises: a) a diaphragm comprised in the deformable membrane; b) a valve seat comprised in the fluidics layer and recessed from a surface of the fluidics layer that contacts the deformable membrane so that the diaphragm does not close the diaphragm valve unless positive pressure is exerted on the diaphragm; and c) a valve inlet and a valve outlet comprised in the fluidics layer and in fluid communication with a fluidic channel; and d) a ram comprised as an actuation element having a forked end comprising tines, wherein the end has a surface complying with the valve seat and wherein the tines are compliant to lateral pressure whereby pressure by the ram on the diaphragm and against the valve seat closes the valve. Embodiments are shown in
Also provided herein is a fluidic device comprising a sample input, a sample output and a waste chamber, all fluidically connected through fluid channels wherein the waste chamber comprises a material that degrades nucleic acid. In one embodiment the material that degrades nucleic acid comprises a hypochlorite salt. In another embodiment the material that degrades nucleic acid comprises an enzyme such as an exonuclease or an endonuclease.
Also provided herein is a fluidic device comprising a fluidic circuit comprising sample input, a reaction chamber and a sample output, wherein the reaction chamber comprises a solid substrate, e.g., solid phase extraction material, for retaining analyte from a sample. In one embodiment the solid substrate comprises a material that binds nucleic acid. In another embodiment the solid substrate comprises Whatman FTA paper, a carboxylated material, a sponge-like material, a polymer membrane, or glass particles. In another embodiment the solid substrate binds a predetermined amount of material. Embodiments are shown in
Also provided herein is a method comprising 1. A method comprising: (a) providing a reaction mixture comprising: (I) a sample comprising mammalian (e.g., human) DNA, (II) reagents for amplifying short tandem repeats (STRs) in the mammalian DNA (e.g., labeled primers, nucleotides and polymerase) and (III) a mammalian-specific probe selected to be amplified in the reaction and including a label that is distinguishable from the labeled primers; (b) performing an STR reaction comprising amplifying STRs in the sample and the mammalian specific probe; (c) detecting an amount of amplified mammalian specific probe in the reaction, e.g., over time, e.g. performing real-time PCR; and (d) optionally, stopping the STR reaction based on the amount of amplified mammalian specific probe detected. In one embodiment the labels are fluorescent labels and the distinguishable label has a wavelength above or below the highest or lowest wavelength of labeled primers. In another embodiment the mammalian specific probe further comprises a quencher such as a Black Hole Quencher® or a Tag Man® probe.
All publications and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.
The novel features of the disclosure are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present disclosure will be obtained by reference to the following detailed description that sets forth illustrative claims, in which the principles of the disclosure are utilized, and the accompanying drawings of which:
I. Instruments for Engaging and Operating Fluidic Cartridges
In one aspect provided herein is a cartridge comprising: (a) a body comprising a fluidic circuit comprising: (1) a sample chamber comprising an opening configured to receive a sample and a via through the body; (2) a reaction chamber; (3) diaphragm valve elements including a valve inlet and a valve outlet, each configured as a via through the body, and a valve seat; (4) a plurality of ports through the body; and (5) one or more fluidic channels in a surface of the body, wherein the one or more fluidic channels put the sample chamber (e.g., through the via), the reaction chamber, the valve elements (e.g., through the valve inlet and valve outlet), and each of the plurality of ports in fluidic communication with each other; (b) a cover layer attached to the body and sealing the via, the fluidic channels and the reaction chamber; and (c) a deformable layer attached to the body, wherein the deformable layer (i) comprises a plurality of holes, each hole communicating with a port; and (ii) in combination with the valve inlet, the valve outlet and the valve seat, form a diaphragm valve configured to regulate fluid flow in the fluidic circuit.
In another aspect provided herein is an instrument comprising: (a) at least one cartridge interface comprising: (1) an engagement assembly having a first position to receive a cartridge as described herein and a second position to engage a received cartridge with a manifold assembly and an optional thermal regulator; (2) a manifold assembly having a plurality of channels, each channel opening onto a front port and a back port, wherein, when the engagement assembly has received a cartridge and is in the second position, a plurality of front ports fluidically engage ports in the cartridge and pneumatically engage the diaphragm of the diaphragm valve, and wherein the deformable material serves as a gasket for fluidic engagement; (3) an optional thermal control assembly (e.g., thermal cycler) configured to place a heat spreader in thermal contact with a thermal cycling chamber of an engaged cartridge and to regulate temperature of the thermal cycling chamber, when the engagement assembly is the second position; (b) a pneumatic and fluidic assembly comprising: (1) a source of pneumatic pressure; (2) a plurality of fluid sources; (3) a plurality of transmission lines connecting a source of pneumatic pressure in each of the plurality of sources in fluid communication with a back port of the manifold assembly; (4) a pump configured to move fluids from the source through the transmission lines.
This configuration permits samples to be inserted into cartridges, and cartridges to be independently inserted into a slot, even if other slots are processing other samples. Thus, in one embodiment, the system can process samples independently.
The cartridge described in
In this embodiment, the cartridge can comprise an injection molded body, for example, a plastic, a deformable film; and a foil, such as a metal foil, each bonded to the body. The body can have integrated alignment features 209 and 225 so that it can be easily and accurately inserted into the interface. The plastic material can include any plastic known to those skilled in the art, such as polypropylene, polystyrene, polyethylene, polyethylene terephthalate, polyester, polyamide, poly(vinylchloride), polycarbonate, polyurethane, polyvinyldiene chloride, cyclic olefin copolymer (COC), or any combination thereof.
The cartridge may be scribed with a barcode or QR code for optical identification or have an EEPROM or RFID or other similar identification device mounted on the cartridge that can assist in sample tracking and optionally contain information about the chemistry, process to be performed, lot number, expiration date, and other information.
The body can have a folding tab 211 that can be snapped shut after the swab is inserted, either by the operator or the system. More than one style of body, each adapted to a swab, punch type, or sample type can be produced. After the tab is snapped shut, the body can serve to contain the sample, providing protection against contamination and facilitating re-testing or recovery of the sample as required.
The body can also define the volumes for two process chambers. The swab, punch, or other sample type is placed in a compartment 207 that also serves as a lysis chamber. To accommodate the swab, punch, or other sample type, it can have a volume ranging from, e.g., 10 μL to 15 mL or 1 ml to 10 ml. Cells are lysed and DNA extracted from the swab, punch, or other sample type in this chamber. The second chamber 301, called the reaction chamber, can serve to capture DNA or house a small amount of lysate for direct amplification. It can also be where cleanup and/or amplification occurs. To minimize the duration of thermocycling and the amount of energy required, this second chamber can have minimal volume, perhaps ranging from 2 μl to 25 μl, although other configurations are practical.
Referring to
The deformable material used in cartridges disclosed herein can be a plastic material (plastic deformation) or an elastic material (elastic deformation). The plastic material can comprise, without limitation, a polymer or a metal. Suitable plastic materials include, without limitation, polypropylene and polyethylene. Suitable metals include aluminum. Suitable elastic materials include, for example, elastomeric materials such a polysiloxanes, e.g., PDMS. Other deformable materials are further described herein.
In one embodiment, the deformable film serves as a gasket for three low-dead-volume connections. These provide an inlet, an outlet, and a purge line that can be used to flush out the cartridge and outlet line. The deformable film also serves as the flexible diaphragm for a valve. The valve seat 305 can be formed into the cartridge body. The inputs 306 and outputs 307 to the valve can be vias through the body, channels between the body and the deformable, or both.
The valve can be actuated by positive or negative pressure or applied to the deformable material over the valve seat. In another embodiment the valve can be actuated by a ram exerting mechanical force on cover sheet 205.
The deformable material may also fill a hole in the body, creating a flexible valve on the far side of the body. The deformable material can be pressed from the near side to deform through the body, and seal against a surface on the far side of the body.
In addition to being mechanically simple, structuring the cartridge around one molded body with functional elements on the surface increases the robustness. Leaks are critical problems, so the fluidic and pneumatic connections need to line up to enable sealing. Tolerance variations accumulate across assemblies, typically with each part contributing some variation. As a result, simpler assemblies can be more robust even with the same part tolerances. Furthermore, the single sample cartridge and other simplified cartridges in this instant disclosure involve only a few connections, further reducing the risk of leakage. Additionally, the effect of thermal expansion increases with size, so having few connections that are also close together reduces the risks still more.
This embodiment integrates parts, reducing materials and assembly costs. In one embodiment, it is designed with pneumatic ports, fluidic ports, and valve controls (either mechanical or pneumatic) onto one side. This simplifies connections and permits more space for other functions such as a temperature regulator (e.g., a thermocycler) to contact the cartridge, an optical system to interrogate the cartridge, or other measurement devices.
Cartridges constructed in this or other embodiments can also be built to accommodate multiple samples. These multi-sample cartridges can permit the operator to run multiple samples without having to insert multiple cartridges. (See, e.g.,
Alternatively, single-sample cartridges can be assembled onto multi-cartridge holders. Operators running many samples at a time are able to take the rack containing used cartridges out of the system and insert the new rack containing unused cartridges. Operators running only a few samples are able to populate only as many spaces as they wish. The remaining spaces can be filled with dummy cartridges or left empty depending on the configuration.
The single-sample cartridges become particularly advantageous when paired with a latched cartridge interface 101 that can permit them to be inserted and removed singly. This can provide more flexible sample flow.
This interface can have a number of cartridge positions 103 or ‘slots’ that can open and close independently. They can apply a compressive force on the cartridges. They can be held closed by solenoids, or another means controlled by the software, or could be manually latched.
An operator with a new sample to run can insert the sample into a cartridge, and snap the cartridge top closed. He or she can then ask the system to open a slot. If a slot is not in use, the system opens it, permitting the sample to be inserted. If a processed cartridge is in the slot, the operator removes it. The operator could read the barcode, QR code, RFID or other identifying material on the cartridge before it is inserted or the system could read as it is inserted or afterwards. The operator can then push the interface closed, and enter additional information if necessary. The system can then start processing the sample immediately or start it automatically when next possible.
In an alternative embodiment, an operator with a new sample can manually open a slot or direct the system to open a slot. If a slot is not in use, the system opens it. If a processed cartridge is in the slot, the operator removes it. The operator inserts a cartridge. The operator could read the barcode, QR code, RFID or other identifying material on the cartridge before it is inserted or the system could read as it is inserted. The operator could then read the barcode, QR code, RFID or other identifying material on the sample if there is one and place the sample into the cartridge in the slot; alternatively the operator could enter sample tracking information manually into the system. After the sample has been added, the top of the cartridge can be closed by the operator or by the instrument. The operator can then push the interface closed manually or the instrument can close the interface. The system can then start processing the sample immediately or start it automatically when next possible.
This instant embodiment can be automated with a loading system that automatically inserts and removes cartridges as needed into slots. The automation can include mechanisms to load samples from a variety of sample types such as a collection of tubes containing swabs, microtiter plates containing liquid samples that can include preprocessing from single sources or mixtures, tubes containing liquid samples that can include preprocessing from single sources or mixtures, blood containers such as Vacutainers, or other containers for additional sample types.
The interface for each cartridge can float, permitting it to seal around the various fluidic and pneumatic connections on one side, while pressing the cartridge against the thermo regulator (e.g., thermocycler) 109 on the other. As shown, the floating interface rotates, pressing the cartridge against a fixed temperature regulator. Alternatively, multiple smaller thermocyclers could be used. These can rotate or translate, possibly pressing the cartridges against a common pneumatic and fluidic assembly.
After processing, the slot may remain closed to avoid contamination.
The interface can press the cartridge against a temperature regulator, e.g., a Peltier device. This contact can be against the foil or film 205 enclosing the reaction chamber. Depending on the chemistry used, on the opposite side of the reaction chamber, the interface can house an LED, filter, and photodiode for reaction quantification or another detector.
In such an embodiment, when the reaction is a short tandem repeat (STR) reaction, in many jurisdictions for casework samples, the amount of human DNA must be quantified. The typical forensic process is to quantify an extracted sample using real time polymerase chain reaction (PCR) in a separate instrument before the sample is STR amplified. In this instant disclosure, a human specific probe is added to the STR mixture which has fluorescence outside the range used by the STR kit. The reaction chamber 301 is interrogated by a suitable wavelength of light for the human specific probe while the STR is being PCR amplified. The human specific probe can be a quencher such as a Black Hole Quencher® or a TaqMan® probe or other chemistries well know to one skilled in the art. As the PCR cycles increase, the fluorescence from the human specific probe is monitored to quantify the amount of human DNA in the reaction. In a preferred embodiment, the number of amplification cycles can be adjusted based upon the amount of human DNA measured; this can be on a cartridge-by-cartridge monitoring if independent thermal cyclers are in use. One advantage is that the human specific probe will allow the concurrent STR amplification to achieve an optimal amplification and produce an amount of STR product that is optimal for the kit regardless of amount of starting DNA in the sample. A second advantage is the real monitoring concurrent with the STR amplification allows integration of a sample-to-answer system without having an additional separate quantification process. A third advantage is for low copy number samples where there is barely enough sample to produce a good STR profile the integration of the quantification with the STR amplification prevents the aliquot typically used for quantification from causing the remaining sample to not have enough DNA for a successful STR amplification.
In addition to actuating the valve diaphragms (e.g., 311, 1301) mechanically, they can be actuated pneumatically. In one embodiment, the interface 1901 (
The interface block 1905 is a component in the interface latch subassembly 1901. The block has alignment features 1911 and 1913 that mate to the cartridge alignment features 2003 and 2005 accurately locating the cartridge in the interface. The block mounts flexibly to a hinge arm 1915 that pivots to engage the cartridge to the interface, or permit the operator to insert or remove cartridges. A frame 1917 loosely guides the cartridge during insertion, ensuring that it can mate with the alignment features.
The single body cartridge allows on-chip storage/integration of reagent reservoir, including, for example, for example, capillary electrophoresis separation gel. This embodiment also permits STR manipulation without having reagents contact PDMS, which can interfere with certain biochemical reactions. This embodiment permits an integrated reaction chamber: The reaction chamber volume is defined by the outside of the fluidic layer and enclosed (e.g., by heat seal plastic, heat seal foil, graphite, etc). It can connect to the circuit either by vias through the fluidics layer, or by enclosed channels along the surface.
In systems that use STR components that are sensitive to PDMS or other diaphragm materials, the second side can house the STR components in reservoirs 603 (
In addition to STR components, other reagents can also be stored on the second side of the fluidics layer. For laminated cartridges, which need to maintain a high degree of flatness near the pneumatic and fluidic circuits, these storage chambers could be above or below the laminate, or off to the side. The storage volumes would need vents near the top, and outlets near the bottom or narrowed sections capable of drawing the fluids upwards like a straw.
To minimize the risk of contamination from one sample to another, reagents that are used before amplification could have separate chambers above the laminated area. Reagents used after amplification, when contamination is less of a risk, can be shared among all sample circuits. This approach permits all reagents needed to run the system to be stored on a single cartridge.
Those reagents which require low pressures for movement or containment can be handled with diaphragm valves. Those reagents which require higher pressures, such as the separation gel, can be drawn out at low pressure into another chamber, and then pushed into the capillaries at high pressure.
II. Cartridges Having a Fluid Distribution Channel
A double-sided fluidic layer offers a number of capabilities for multi-sample cartridges, such as an embodiment as shown in
III. Pneumatic Channels to Selectively Block Diaphragm Valves
The right-to-left channels can also route pneumatic control signals to enable or disable specific circuits, as shown in
IV. Cartridges Having a Deformable Layer Sealed to a Plastic Body
Cartridges of this disclosure can have a body comprising a solid material. The solid material can be rigid, plastic (capable of irreversible deformation) or elastic (capable or reversible deformation). The body can be stiff or compliant. In some embodiments, the solid material is a polymer, e.g., a thermoplastic, such as polypropylene. The body can comprise an external surface comprising elements of fluidic circuits, such as channels, compartments, vias and valve seats. The body can be made by injection molding of the thermoplastic. These features can be covered with a layer of material attached to the surface of the cartridge body. The layer can function to seal otherwise open features such as channels and compartments. The material can be a deformable material that can deform to contact a valve seat, thereby closing the valve. In certain embodiments, the solid material is inelastic (not capable of elastic deformation). For example, the solid material is not an elastomer, such as PDMS.
The material can be attached to the surface of the body using a selective bonding process in which the material bonds to selected portions of the surface during the bonding process and does not bond to un-selected portions of the circuit after the bonding process is complete. For example, the material may bond to surfaces other than fluidic elements during the bonding process, and not bond to fluidic elements, such as channels and valve seats, after the bonding process. Methods for selective bonding include, for example, thermal bonding (e.g., heat sealing, welding, laser welding), chemical bonding (e.g., chemical bonding of oxide to PDMS) and selectively placed adhesives.
In one embodiment a layer of the deformable material is attached to a surface of a cartridge body through thermal bonding. This can include thermally bonding the material directly to the surface, or thermally bonding the material through an intermediate layer of material. In the latter case the material can be a laminate in which a deformable material is coated with a layer of material that contacts the surface and that melts at lower temperature. In either case bonding typically comprises contacting the deformable material to the body to form a combination and using a die to apply heat and pressure to the combination. Application of heat and pressure melts substrates in locations at which the material and body are in contact and fuse them, e.g., through coalescence. This process is more generally referred to as welding.
A material that bonds to a body through application of heat and pressure is referred to herein as “heat seal”. Heat seals are well known in the art and are commercially available. For example, 4titude (Walton, Surrey, UK) commercializes a variety of heat seals. These heat seals are described on the website 4ti.co.uk/sealing/heat-seals/, which is incorporated herein by specific reference. These include, for example, Clear Seal, Clear Weld Seal and Foil Seal. Clear Seal is described as “an optically clear laminate film forming a peelable seal to polypropylene, polyethylene, polystyrene, polycarbonate and cyclic olefin copolymer (COC) plates . . . . The optical clarity of this seal enables its use for sealing plates required for imaging use, including fluorescent detection methods such as qPCR and colorimetric assays . . . . The Clear Heat Seal forms a complete seal to a plate enabling both low temperature uses, including low temperature storage, and high temperature uses, such as PCR (when used with a pressurized heated lid) . . . . This seal demonstrates moderate solvent resistance and can be utilized for short term compound storage at room temperature.” Clear Weld Seal is described as “an optically clear polymer film forming a permanent seal to polypropylene plates . . . . The optical clarity of this seal enables its use for sealing plates required for imaging use, including fluorescent detection methods such as qPCR and colorimetric assays . . . . The Clear Weld Heat Seal Mark 2 forms a complete seal to a plate, enabling both low and very high temperature uses, including low temperature storage and high temperature incubations . . . . This seal is suitable for PCR/qPCR, even without the use of a pressurized heated lid, and is 100% effective when used in water bath thermal cyclers . . . . The permanent nature of this 100% effective seal renders it suitable for the storage and disposal of hazardous material . . . . Clear Weld Heat Seal Mark 2 demonstrates a good solvent resistance and can be utilized for long term compound storage.” Foil Seal is described as being “compatible with polypropylene and polystyrene plates . . . . This seal demonstrates moderate solvent resistance and can be used for low temperature compound storage in DMSO and organic solvents and short term room temperature storage . . . . The Foil Heat Seal can be pierced with a pipette tip, manually or by liquid handling robots, or it can be removed by peeling . . . . It can be resealed by applying another Foil Heat Seal directly on top of a previously pierced seal.” Heat seals also are produced by Axygen, a Corning brand (Corning, Tewksbury, Mass., USA). These include Axygen® PlateMax heat sealing film which is “designed for applications ranging from PCR and Real-Time PCR to ELISA and cell culture . . . . They can be used in wide temperature ranges, making them suitable for many applications . . . . The Axygen PlateMax semi-automatic plate sealer is ideal for the low to medium throughput laboratory that requires uniform and consistent sealing of microplates. Offering complete versatility, the PlateMax sealer will accept a wide range of plates for PCR, assays, or storage. It can be used to seal a wide range of plate heights,” and sealing film rolls, which are described as being “designed for applications ranging from PCR and Real-time PCR to storage. They can be used in a wide range of temperatures making them suitable for nearly any application. See the website: catalog2.corning.com/LifeSciences/en-US/Shopping/Product.aspx?category name=Genomics+and+Proteomics(Lifesciences)%7cPCR+Products(Lifesciences)%7cSealing+Films+and+Tapes+for+Microplates(Lifesciences)%7cHeat+Sealing+Films+and+Tapes+for+Microplates(Lifesciences), which is incorporated herein by specific reference.
The deformable material can be a homogenous or non homogenous material. In certain embodiments, the heat seal material is made from the same material as the body of the cartridge. It can comprise a thermoplastic (e.g., polypropylene, polyethylene, polystyrene, cycloolefin co-polymer (COC), mylar, polyacetate) or a metal (e.g., aluminum). See, e.g., WO 2012/136333, which is incorporated herein by specific reference. The heat seal can be produced by contacting a heat seal layer with the body and applying heat and pressure. Non-homogenous films include laminates having a first side for contact with the heater and a second side for contact with the body. The first side has higher melting temperature (“high melt”) than the second side (‘low melt”). This permits use of a heat source to bring the lower side to its melting temperature before the first side allowing bonding to the body without bonding to the heater.
In the single sample cartridge, one side of the body into which compartments are formed is covered in a film or foil that can be adhered or thermally attached to the body. This encloses a second functional layer while only requiring one molded part. This permits functional details—valves, channels, etc. on different sides of the body. In the case of the single sample cartridge, this permits the valves, pneumatic connections, and fluidic connections to be on one side of the cartridge, while the reaction chamber is on a different side of the cartridge. As a result, the temperature regulator controlling the reaction chamber temperature can do so through a thin film, rather than the deformable gasket, which can result in quicker and more controlled thermocycling.
Referring to
Depending on the film used, there can be a slight overhang around the perimeter of the valve, channel, or volume. This overhang can be due to adhesive or plastic flow during bonding. To prevent these from affecting the quality of the valve seal, the valve inlet, outlet, or both can be through vias in the valve seat 1317. The valve seat, away from the perimeter, can be less affected by the overhang.
Because of the limited flexibility of the film, it may be necessary to create a dimple over the valves. This can be achieved by coining the film downwards against the valve, with the limitation that the heat applied must not be enough to bond the film to the valve seat. A preferred approach would be to vacuform the dimples. The ordinary process of heat sealing involves applying a combination of heat and pressure to create a bond. If the heated tool (1401) was made from a porous material and had cavities cut above the valves, suction could be applied that can draw the film over the valve into the cavity, creating a dimple. This can occur at the same time as the film was being bonded to the body in other areas.
This embodiment can allow multiple fabrication and material options. For example, PDMS, which is commonly used in microfluidics, could be replaced with such a material, such as the heat seal films. This embodiment also reduces requirement for flatness in pieces, permitting other cartridge materials, such as polypropylene.
The use of the fluidics layer for reagent storage and the use of sections of the enclosing film for shipping as in the embodiment of 601, and the use of sections of the enclosing film to implement valves as in the embodiment of 701, permits the cartridge functions to be served by one molded piece and one or more bonded films. Another embodiment, as shown in
V. Clamp-Sealed Cartridges
To close off the outlets 1109 and vents 1105 to these reagent chambers 1107, bar clamps 1103 can be built into the shipping container for the cartridge. These bar clamps can have some rigidity, but can be covered by a deformable or other material that can conform to the cartridge surface. It may have a basic shape or be formed to mate with the cartridge surface.
Bar clamp 1103 is able to hold the seal film cover (907, not shown in
If the flexible bar clamp is U-shaped as shown, it can close each channel in two places to prevent leakage. The operator will then be able to confirm that no leakage has taken place by examining the area between the two seals. Any leakage past both seals will generally leave a residual amount between the two seals.
Before use, the two vias leading to each of the reagent reservoirs are held closed by a shipping clamp. This shipping clamp can apply a uniform force to a flexible pad, causing the pad to deform and hold the valves closed. Alternatively, it can include a number of small rubber contacts that can individually hold each valve closed. This shipping clamp can then be removed before the cartridge is inserted into the system.
VI. Diaphragm Valve with a Bossed Diaphragm
Diaphragm valves also can be actuated mechanically using a ram, e.g., a pin. These can be actuated by a solenoid. If actuated by solenoid, it may be beneficial to add a boss (such as element 1511) to the outside of the deformable. This permits a ram to push against the boss, creating a centered force sealing the valve, even if the solenoid is not centered over the valve.
VII. Turret Cartridge
Cartridges actuated mainly by a syringe pump or by a manually operated syringe are included in this instant disclosure. The cartridges can be controlled by motors controlled by the computer on the system.
One embodiment of a cartridge utilizes a syringe pump for actuation, with selectable, specialized areas arranged in a ring. These areas can each store reagents, house the swab or punch, contact a temperature regulator, connect to the capillary for separation, etc.
Referring to
One, two or three positions on the hub can be temperature controlled. One position on the hub can be open on top, for sample insertion. One or more positions can have external, retractable magnets.
Turret cambers can include: (A) Vent: air to injection chamber; (B) Vent: to denature heater; (C) lysis chamber/swab vial; (D) lysis buffer/Waste; (E) mix chamber/beads; (F) water; (G) ethanol; (H) STR lyosphere (amplification reagents); (I) capture solution and size standard (or lyosphere); (J) eluting agent; (K) electrophoresis separation gel; (L) reaction chamber.
Gel injection may be to a booster pump instead of directly to the capillary. This would avoid the need for high-pressure seals, in the sample cartridge (This would permit gel injection in parallel with other functions.) If the capillary can be mounted directly, denature heading may be complete by one of the heated positions, without an external denature heater. An external waste gate, at the cathode end of the capillary may still be necessary.
This embodiment can permit an interface consisting of one rotary and one stepper motor, eliminating pneumatic pumps, manifolds, anode module/gel filling mechanism, etc.
VIII. Lead-in Guiding Fluid Delivery Pogo Pin
IX. Vent Tabs
In an embodiment shown in
For cartridge concepts that have bodies 1205 and moveable reagent reservoirs 1201, such as the cartridge of the embodiment of 701, there is another approach to providing vents. This approach does not require additional parts. This is to build in designed-to-fail tabs (e.g., 1203) into the reagent reservoir. Before use, these tabs will remain closed, but will have a slight interference with the cartridge body 1205. When the reagent reservoir is engaged by pressing, these tabs will try to pull away from the main volume. It will tear or crack, opening a small vent in the reservoir.
This embodiment can provide a vent for on-cartridge reagent reservoirs without requiring additional degree of freedom in the interface or additional part in the cartridge.
The cartridge can utilize off-cartridge pumps to move liquids.
To avoid the need for high mechanical precision, these valves and channels can be larger than traditional microfluidic valves and channels.
X. Fluidic Device with Diaphragm Valve
The cartridge of this disclosure can include diaphragm valves. Diaphragm valve can be formed having a valve chamber in the fluidics layer of the cartridge and a deformable membrane attached to a surface of the fluidics layer and providing a diaphragm for opening and closing valve. In one embodiment, the valves are normally open. That is, at ambient pressure the valve is open and closing the valve involves applying positive pressure to the diaphragm opposite the valve seat. Applying negative pressure to the diaphragm opposite the valve seat can further open the valve. The diaphragm can be actuated by pneumatic or mechanical pressure. In an embodiment of this disclosure the diaphragm is mechanically actuated by positive pressure applied by a ram or rod having an end configured for insertion into the valve chamber. In certain embodiments the rod has a compliant end that promotes contact between the diaphragm and a valve seat, thereby sealing the valve closed. Withdrawal of the rod relieves pressure on the diaphragm, thereby opening the valve.
In one embodiment of a normally open valve, a surface of the fluidics layer comprises a recess that both defines a valve chamber and functions as a valve seat. At ambient pressure the deformable membrane does not sit against the valve seat and the valve is in an open configuration. Positive pressure on the deformable membrane from the side opposite the fluidics layer pushes the deformable membrane against the valve seat, closing the valve. The valve seat can take a curved shape that is convex with respect to the surface of the fluidic layer, against which the deformable membrane can conform. For example, the valve shape can be a section of a sphere or an inverted dimple or a dome. Such a configuration decreases the dead volume of the valve, e.g., by not including a valve chamber that contains liquid while the valve is closed. This valve also comprises a surface against which the deformable membrane can conform easily to close the valve. In another embodiment, the concave surface can comprise within it a sub-section having a convex surface, e.g., an inverted dimple comprising an extraverted dimple within it forming, for example, a saddle shape. The convex area rises up to meet the deformable membrane under pressure, creating a better seal for the valve.
Valve seats can be recessed with respect to the rest of the surface by about 25 microns to about 1000 microns, e.g., about 700 microns. Valves can communicate with fluidic channels that are either microfluidic or macrofluidic (e.g., having an aspect less than 500 microns or having an aspect greater than 500 microns or at least 1000 microns). In certain embodiments of a normally open valve, the concavity is recessed less than the channels to which it is connected. In certain embodiments the channels can enter partially into the concavity, for example forming a vault. In certain embodiments, the channels and concavity are formed by micromachining, injection molding or embossing.
XI. Valve Actuated by Ram with Compliant End
One embodiment involves closing a fluidic valve with a ram. The valve can be comprised in substrate that contains the valve and one or more input and output fluidic channels. There can be more than one input and output. These channels can enter the surface of the dome valve at any location on the surface as long as there is a sealing surface between channels. In certain embodiments, channels can enter the valve chamber through vias that connect with the channels. The dome valve is then covered with a membrane either elastic or non-elastic film. The film is affixed to the perimeter of the dome to create an air and liquid tight seal. The ram is then pressed against the film diaphragm with sufficient force to deform the diaphragm and press the film onto the dome surface. The pressure from the ram creates a fluidic seal between the orifices of the ports entering the dome valve.
In one embodiment the valve is configured as a router. The router can have, for example, four inlets/outlets. In this configuration the forked ram, when engaged, can block access to the router by some, but not all, of the inlets/outlets. For example, the forked ram could allow fluid flow through the router in a north-south direction or not it in an East-West direction.
The ram is structured such that there are one or more flexure posts defining an identical dome surface to match the valve dome surface with the offset of the thickness of the diaphragm. The flexure posts with the seal seat tips will be able to self align to the target seal areas of the dome, namely the perimeter of the orifice for the input and output channels of the valve. The flexure posts also concentrate the stress generated by the force applied to the overall post onto the active seal area.
Referring to
XII. Reaction Chamber
In one embodiment a fluidic device of this disclosure comprises a reaction chamber that comprises a solid substrate, e.g., solid phase extraction material, for retaining analyte from the sample. The solid substrate can comprise a material that binds the analyte, such as a nucleic acid such as DNA. The amount of solid substrate in a chamber and the selected to retain the predefined amount of analyte. For example, the material can be a Whatman FTA paper or a carboxylated material. Alternatively, the solid substrate can be an absorbent or sponge-like material that absorbs a predetermined volume of fluid. The material can be in the form of a monolith. The material can be, for example, PVDF (polyvinyldiene fluoride) membranes, filter paper, glass particles, silica, or other solid phase extraction material. In operation, lysate is pumped through the chamber and a predetermined amount of analyte is retained on a solid substrate. Then, retained material is contacted with reagents, e.g., reagents for PCR. The resulting material can be incubated to form a reaction product. For example, the chamber can be put into thermal contact with a thermal-control device, such as a Peltier, and the reaction mixture can be thermal cycled. In another embodiment, the chamber can include a pocket or container designed to retain the defined volume of liquid.
XIII. Contaminant Deactivation
In one embodiment the fluidic layer includes a waste chamber. A waste chamber can contain material that degrades nucleic acids, polypeptides, or other analytes. For example a material can comprise a chlorinated material, such as calcium hypochlorite. Alternatively, the waste chamber can include in absorbent material that absorbs waste containing liquid
In another embodiment the nucleic acid degrading material is contained in a water-soluble capsules in yet another embodiment the nucleic acid degrading material is combined with an absorbent material such as cellulose or polypropylene fibers.
In another embodiment, the waste chamber contains enzymes that degrade the nucleic acids (e.g., nucleases), polypeptides (e.g., proteases), or other analytes such as phosphorylated sites (e.g. phosphatases).
XIV. Cartridge and Method
XV. Integrated System
The cartridges of this disclosure are useful in integrated and automated sample-to-answer systems that, starting from a sample comprising biological material, generate an analysis of the sample. In certain embodiments, the biological material is DNA and the genetic profile involves determining one or a plurality of alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, a STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
Systems provided herein may be fully integrated. Sample processing can be accomplished in a single system without having to remove a sample and transfer it to another system. Systems provided herein can be fully automated, enabling a user to process a sample without substantial input from the user.
A sample preparation module includes a cartridge module assembly configured to engage and operate one or more than one sample cartridge. A sample cartridge is configured to receive one or more samples and to perform nucleic acid extraction and isolation, and DNA amplification when the cartridge is engaged with a cartridge module assembly in the system. It can also include controls and standards for assisting in analysis. In other embodiments, a sample cartridge is configured to receive one or more samples and to perform cell lysis, and enzymatic assays when the cartridge is engaged with a cartridge module assembly in the system.
The sample preparation module can include a receptacle for receiving one or more cartridges, an engagement assembly to engage the cartridge; a fluidic manifold configured to engage ports in a cartridge and to deliver pressure and/or fluids to the cartridge through the ports; a delivery assembly configured to deliver reagents, such as amplification pre-mix, from a compartment in the sample cartridge to an amplification compartment; a pneumatic manifold configured to engage ports in a cartridge and to deliver positive or negative pressure to the cartridge through the ports for moving fluids and operating valves, pumps and routers in the cartridge; a pump configured to deliver pressure to the fluidic and pneumatic manifold. Consumable reagents can be carried in a module, e.g., a buffer module, that is, removably engageable with the cartridge module.
PCR can be carried out using a thermal cycler assembly. This assembly can include thermal controller, such as a Peltier device, infrared radiation source, resistive heating element, circulating water or other fluids, circulating air, movement of constant temperature blocks, or other material, which can be configured to heat and cool for thermal cycling and can be comprised in the cartridge module which can be configured to move the thermal controller into thermal contact with the thermal cycling chambers, for example, through a heat spreader (or thermoconductor that can spread/distribute heat and cooling) disposed over each of the reaction chambers. In some embodiments, the cartridge comprises a temperature regulator assembly having one or more (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 16, 24, 32, 40, 48 or more) thermocycling chambers and the sample cartridge can be in fluid communication with a fluidic channel.
An analysis and detection module is configured to receive analyte from the sample preparation module and perform capillary electrophoresis on the analyte to detect analytes separated by electrophoresis and to analyze the detected analytes. It can include a capillary electrophoresis assembly, a detection assembly, and an analysis assembly.
The capillary electrophoresis assembly can include an injection assembly, that can include a denature heater assembly, a positioning assembly for positioning an analyte for capillary injection; a cathode assembly; a capillary assembly; an anode assembly; a capillary filling assembly for filling a capillary with separation medium and a power source for applying a voltage between the anode and the cathode.
A detection assembly can comprise a laser configured to illuminate the capillaries and a detector. The laser can be configured to excite fluorescent dyes in the analyte. In alternative embodiments, the laser can be replaced by an alternate light source such as an LED. The detector can include a CCD array, photomultiplier, diode array, or other detector, for detecting light produced by excited dyes and for producing an output signal.
An analysis assembly can include a computer comprising memory and a processor for executing code (e.g., code on a tangible medium) for analyzing the output signal and producing a computer file containing an analysis of the signal. Such an analysis can include, for example, identification of alleles from various STR loci. The computer file can be in a format that is compatible with public databases. For example, the file can be in CODIS format which is compatible with the National DNA Index System (NDIS) operated by the FBI.
The system can be operated by a control module. The control module can include a user interface configured to receive instructions from and deliver information to a user. It can include software programmed to execute routines for performing the operations mentioned, above, and transmit and receive information, such as computer files, from remote locations, for example, over the internet.
XVI. Method of Use
The cartridges of this disclosure can be used in an integrated system for preparing a sample, for example, DNA isolation and amplification. For example, in one embodiment, a sample contained on for example a swab or a card punch, can be introduced into sample chamber 207. The chamber can be snapped shut by the lid 211. The cartridge can be engaged with cartridge interface 103. Cell lysis buffer contained in an on-system reservoir can be feed through line 405 through interface assembly 401 into the fluidic channel in the cartridge and into the sample chamber 207. After lysis, lysate can be moved through a fluidic channel on the chip, for example, which pumps the fluid into a reaction chamber 301. In one embodiment, the DNA reaction chamber can include magnetically attractable particles that bind DNA and that can be immobilized in the reaction chamber by applying a magnetic force generated in the interface. This can eliminate the need for an intermediate DNA isolation chamber. Waste fluid can be moved through the cartridge and out through a vent. Reagents for performing PCR or other reactions can introduced into the reaction chamber through one of the fluid lines 405 connected to the interface. A thermal control mechanism in the system can apply heat to perform thermal cycling in a thermal cycling chamber 301 of the cartridge. In some embodiments the heat is applied to a heat transmission element, for example, a foil or metalized film, that improves thermal contact and transmission.
The cartridges of this disclosure can be used in an integrated system for analyzing a sample, for example, DNA isolation and amplification with real time or end point detection. For real time measurement, the samples can be interrogated by an optical detection system while amplifying in reaction chamber 301. The readout can be the change in fluorescence or by melting point. The probes can be human specific for human identification, forensics, or molecular diagnostic applications, or specific for pathogens for molecular diagnostic applications, or for bioagents for biodefense applications or nonspecific intercalators for determining amount of DNA present. Amplification methods include, for example, thermal or isothermal amplification reactions, for example, PCR, rolling circle amplification, whole genome amplification, nucleic acid sequence-based amplification, and single strand displacement amplification, single primer isothermal linear amplification (SPIA), loop-mediated isothermal amplification, ligation-mediated rolling circle amplification and the like
The cartridges of this disclosure can be used in an integrated system for analyzing a sample. The assay can detect a polypeptide (e.g., immunoassay) or a nucleic acid (e.g., PCR). The assay can be multiplex or single analyte. They can involve any assay to measure presence, amount, activity, or other characteristics of the sample. These include assays that involve detection by fluorescence, luminescence, chemiluminescence, Raman, absorbance, reflectance, transmittance, birefringence, refractive index, colorimetric and combinations thereof. In this instant disclosure, the enzyme master mix and the substrate might be individually added to the reaction and the progress or endpoint of the assay monitored optically.
For STR applications, after thermal cycling, other reagents such as molecular weight markers (size standards) can be combined with the PCR product. Movement through the cartridge can be controlled when diaphragm valve 303 is actuated by pneumatic or mechanical actuators wherein forces transmitted through line 407. Products of the PCR can be moved off chip for analysis through an output line.
While preferred claims of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such claims are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the claims of the invention described herein may be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.
This application is a divisional of and claims priority to U.S. application Ser. No. 15/037,039, filed May 16, 2016, which is a National Phase Application of PCT/US14/66008, filed Nov. 17, 2014, which claims the benefit of U.S. provisional application 61/905,804, filed Nov. 18, 2013 and of U.S. provisional application 61/981,678, filed Apr. 14, 2014, each of which is incorporated by reference in its entirety.
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| Number | Date | Country | |
|---|---|---|---|
| 20190242917 A1 | Aug 2019 | US |
| Number | Date | Country | |
|---|---|---|---|
| 61981678 | Apr 2014 | US | |
| 61905804 | Nov 2013 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 15037039 | US | |
| Child | 16258412 | US |
