Claims
- 1. An isolated DNA sequence encoding a prepro form of Paramecium tetraurelia cathepsin L wherein the DNA sequence comprises nucleotide sequence regions that encode the amino acid sequence regions represented by (i) positions 43 through 57, inclusive, (ii) positions 110 through 122, inclusive, and, (iii) positions 194 through 201, inclusive, of SEQ. ID. No 13.
- 2. An isolated DNA sequence comprising the sequence of SEQ. ID. No. 12.
- 3. An isolated DNA sequence comprising the sequence complementary to the sequence of SEQ. ID. No. 12.
- 4. An isolated DNA sequence comprising a nucleotide sequence that encodes the Paramecium tetraurelia cathepsin L signal sequence having the amino acid sequence represented by positions 1 through 22, inclusive, of SEQ. ID. No 13.
- 5. An isolated DNA sequence comprising nucleotide positions 1 to 86 of the sequence of SEQ. ID. No. 12.
- 6. An isolated DNA sequence comprising the sequence complementary to the sequence as claimed in claim 5.
- 7. An isolated DNA sequence comprising nucleotide positions 21 to 86, inclusive, of the sequence of SEQ. ID. No. 12.
- 8. An isolated DNA sequence comprising the sequence complementary to the sequence as claimed in claim 7.
- 9. The isolated DNA sequence as claimed in claim 7, wherein all TAA codons are replaced with a codon selected from the group consisting of CAA and CAG and specifying the amino acid glutamine.
- 10. The isolated DNA sequence as claimed in claim 9, wherein all TAA codons are replaced with CAA codons.
- 11. An isolated DNA sequence comprising a nucleotide sequence that encodes the Paramecium tetraurelia cathepsin L propeptide having the amino acid sequence represented by positions 23 through 109, inclusive, of SEQ. ID. No 13.
- 12. An isolated pro region-encoding DNA sequence comprising nucleotide positions 87 to 347, inclusive, of the sequence of SEQ. ID. No. 12.
- 13. An isolated DNA sequence comprising the sequence complementary to the sequence claimed in claim 12.
- 14. The isolated DNA sequence as claimed in claim 12, wherein all TAA codons are replaced with a codon selected from the group consisting of CAA and CAG and specifying the amino acid glutamine.
- 15. The isolated pro region-encoding DNA sequence as claimed in claim 14, wherein all the TAA codons are replaced with CAA codons.
- 16. An isolated DNA sequence comprising nucleotide positions 348 to 1276, inclusive, of the sequence of SEQ. ID. No 12.
- 17. An isolated DNA sequence comprising nucleotide positions 348 to 965, inclusive, of the sequence of SEQ. ID. No 12.
- 18. The isolated DNA sequence as claimed in claim 17, wherein all TAA codons are replaced with a codon selected from the group consisting of CAA and CAG and specifying the amino acid glutamine.
- 19. The isolated DNA sequence as claimed in claim 18, wherein all the TAA codons are replaced with CAA codons.
- 20. A process for preparing a cathepsin L propeptide which comprises expressing the propeptide-encoding DNA sequence of claim 14 in a heterologous expression system.
- 21. The process as claimed in claim 20, wherein the expression system is E. coli.
- 22. The process as claimed in claim 21, wherein a DNA sequence comprising nucleotide positions 87 to 347, inclusive, of the sequence of SEQ. ID. No. 12, wherein all the TAA codons are replaced with CAA codons, is introduced into E. coli using a heat-inducible vector.
- 23. A process for preparing a cathepsin L which comprises expressing a cathepsin L-encoding DNA sequence comprising nucleotide positions 348 to 965, inclusive, of the sequence of SEQ. ID. No 12 in a heterologous expression system.
Priority Claims (1)
Number |
Date |
Country |
Kind |
196 19 366 |
May 1996 |
DE |
|
RELATED APPLICATIONS
This application is a divisional of application U.S. Ser. No. 08/981,957, filed on Apr. 13 1998, now allowed, which was filed pursuant to 37 CFR §371 from international application PCT/EP97/02388, filed on May 9, 1997 now U.S. Pat No. 6,312,935, and now published as WO 97/43425, which in turn claims priority to German application 196 19 366.4, filed May 14, 1996.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
6312935 |
Kiy et al. |
Nov 2001 |
B1 |
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Entry |
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