Patent Application
20240109973
The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Dec. 8, 2021, is named MSKCC_048_WO1_SL.txt and is 17,823 bytes in size.
All references cited in this disclosure are hereby incorporated by reference in their entireties (numbers in parentheses or in superscript following text in this patent disclosure refer to the numbered references provided in the “Reference List” section of this patent specification). In addition, any manufacturers' instructions or catalogues for any products cited or mentioned herein are incorporated by reference. Documents incorporated by reference into this text, or any teachings therein, can be used in the practice of the present invention.
Immunotherapy with immune checkpoint blockade (anti-CTLA4, anti-PD-1/PD-L1) has had a major impact on cancer treatment.1 Despite this success, the majority of cancer patients (over 80%) still do not benefit from it. A major hurdle to overcome resistance to immune checkpoint blockade is proper activation of antigen presenting cells (APCs), particularly dendritic cells (DCs), which are responsible for priming T cells. Many cancers (including breast2 colorectal3 lung4 and prostate5 cancer) demonstrate defective APC activation. Since APCs are responsible for priming T cells, this may at least partly explain the absence of a productive anti-tumor T cell response in immunotherapy-refractory cancers.
Stimulation of CD40 on APCs has been recognized as a highly effective way to activate APCs.6,7 Indeed, CD40 agonists have demonstrated antitumor activity as monotherapy in human cancers.8-10 Currently there is great interest in the use of CD40 stimulation in the treatment of pancreatic cancer, in part because of compelling early-phase data incorporating the CD40 agonist mAb APX005M (developed by Apexigen) in the first-line treatment of metastatic pancreatic cancer.11 And in studies performed in mice, it has been shown that a CD40 agonist antibody can be used to overcome resistance to PD-1 blockade and that combining CD40 activation with TLR4 stimulation using monophosphoryl lipid A (MPL) can restore sensitivity to PD-1 blockade, confer persistent antitumor immunity, and overcome the challenge of T-cell exhaustion in tumors.12
It has been demonstrated that CD40 agonist antibodies with a modified Fc portion that increases binding to a specific Fc receptor—FcγRIIB, present on intratumoral myeloid cells—can more potently stimulate APCs.13 This has led to the production of a series of CD40 agonist antibodies designed to have increased FcγRIIB binding, including the antibodies referred to as APX005M14 and 2141-V1115. However, the utility of such antibodies is dependent on specific defined populations of tumor-infiltrating immune cells that vary widely between patients.16 Thus, there remains a need in the art for agents that stimulate CD40 and enhance APC activation-independent of the presence of patient-specific populations of tumor-infiltrating immune cells. The present invention addresses this need.
The present invention provides novel CD40 binding molecules that were designed specifically to compete with CD40's ligand (CD40L) for binding to CD40 and to have intrinsic CD40 agonist activity on antigen-presenting cells without a requirement for Fc receptor engagement. The process by which these CD40 binding molecules were developed is described in the Examples section of this patent disclosure, and the amino acid sequences of several of these CD40 binding molecules (and their key binding determinants, including their CDRs) are provided in the Detailed Description section of this patent disclosure. As described further in the Examples section of this patent disclosure, these novel CD40 binding molecules exhibit robust antitumor activity in vivo in a clinically relevant model of melanoma-without dependence on Fc receptor engagement or intratumoral immune cell populations that vary greatly between patients.
Accordingly, in some embodiments the present invention provides isolated CD40 binding molecules comprising: the complementarity-determining region (CDR) H1, CDR H2 and CDR H3 regions of SEQ ID NO. 1, and the CDR L1, CDR L2, and CDR L3 regions of SEQ ID NO. 2.
Similarly, in some embodiments the present invention provides isolated CD40 binding molecules comprising: (i) the CDR H1 and CDR H2 regions of SEQ ID NO. 1, (ii) the CDR H3 region of SEQ ID NO. 1, 3, 5, 7, or 17, and (iii) the CDR L1, CDR L2, and CDR L3 regions of SEQ ID NO. 2.
And, in other embodiments the present invention provides isolated CD40 binding molecules comprising: (i) the CDR H1 and CDR H2 regions of SEQ ID NO. 1, (ii) a CDR H3 region comprising SEQ ID NO. 16, and (iii) the CDR L1, CDR L2, and CDR L3 regions of SEQ ID NO. 2.
In further embodiments the present invention provides isolated CD40 binding molecules comprising: (i) a heavy chain variable region comprising: a CDR H1 domain comprising SEQ ID NO. 9, a CDR H2 domain comprising SEQ ID NO. 10, and a CDR H3 domain comprising SEQ ID NO. 16, and (ii) a light chain variable region comprising: a CDR L1 domain comprising SEQ ID NO. 12, a CDR L2 domain comprising the amino acid sequence FTS, and a CDR L3 domain comprising SEQ ID NO. 13. In some such embodiments the CDR H3 domain in the CD40 binding molecule comprises SEQ ID NO. 11 or SEQ ID NO. 14 or SEQ ID NO. 15. In some such embodiments the CDR H3 domain in the CD40 binding molecule comprises SEQ ID NO. 11. In some such embodiments the CDR H3 domain in the CD40 binding molecule comprises SEQ ID NO. 14. In some such embodiments the CDR H3 domain in the CD40 binding molecule comprises SEQ ID NO. 15.
In some embodiments the present invention provides isolated CD40 binding molecules that comprise (a) a heavy chain variable region comprising SEQ ID NO. 3, SEQ ID NO. 5, or SEQ ID NO. 7, and (b) a light chain variable region comprising SEQ ID NO. 4, SEQ ID NO. 6, or SEQ ID NO. 8—all of which variable region sequences are humanized.
In some embodiments the present invention provides CD40 binding molecules that comprise (a) a heavy chain variable region comprising amino acids 1-121 of SEQ ID NO. 1, and (b) a light chain variable region comprising amino acids 1-113 of SEQ ID NO. 2—which variable region sequences are humanized.
In some embodiments the present invention provides CD40 binding molecules that comprise (a) a heavy chain variable region comprising SEQ ID NO. 17 and (b) a light chain variable region comprising SEQ ID NO. 18-which variable region sequences are murine.
In some embodiments the CD40 binding molecules further comprise a human constant region. In some such embodiments such CD40 binding molecules further comprise a human IgG constant region. In some such embodiments such CD40 binding molecules further comprise a human IgG2 constant region.
In some embodiments the CD40 binding molecules comprise a heavy chain comprising SEQ ID NO. 1, and a light chain comprising SEQ ID NO. 2.
The above sequences, as identified by their SEQ ID NOs, are provided in Tables A & B in the Detailed Description section of this patent disclosure.
In some embodiments the CD40 binding molecules described above or elsewhere herein are, or comprise, antibodies. For example, in some embodiments the CD40 binding molecules are, or comprise, humanized antibodies, fully human antibodies, chimeric antibodies, bi-specific antibodies, or multi-specific antibodies.
In some embodiments the CD40 binding molecules described above or elsewhere herein are, or comprise, antibody fragments. Examples of such antibody fragments include, but are not limited to, Fv, Fab, F(ab′)2, Fab′, single chain Fv (scFv), sc(Fv)2, and disulfide-linked (dsFv) fragments.
In some embodiments the CD40 binding molecules described above or elsewhere herein are, or comprise diabodies, nanobodies, triabodies, tetrabodies, nanobodies, or minibodies.
In those embodiments where a CD40 binding molecule as described above or elsewhere herein comprises a heavy chain constant region, or a portion thereof, the heavy-chain constant region may be an alpha, delta, epsilon, gamma, or mu heavy chain constant region.
Similarly, in some embodiments, the CD40 binding molecules described herein may be, or may comprise, an IgA, IgD, IgE, IgG or IgM class immunoglobulin molecule. In some embodiments, the CD40 binding molecules described herein may be, or may comprise, an IgG1, IgG2, IgG3, or IgG4, class immunoglobulin molecule. In some embodiments, the CD40 binding molecules described herein may be, or may comprise, an IgG1 or IgG2 class immunoglobulin molecule. In some embodiments, the CD40 binding molecules described herein may be, or may comprise, an IgG2 class immunoglobulin molecule.
In those embodiments where a CD40 binding molecule as described above or elsewhere herein comprises a light chain constant region, or a portion thereof, the light-chain constant region may a lambda light chain constant region or a kappa light chain constant region.
In some embodiments, the present invention also provides isolated CD40 binding molecules that bind to the same epitope on human CD40 as any one of the CD40 binding molecules described above or elsewhere herein. Similarly, in some embodiments the present invention provides isolated CD40 binding molecules that compete with any one of the CD40 binding molecules described above or elsewhere herein for binding to human CD40.
In addition to the various CD40 binding molecules described herein, the present invention also provides various compositions comprising such CD40 binding molecules, for example pharmaceutical compositions that comprise a CD40 binding molecule as described herein and a pharmaceutically acceptable carrier.
The present invention also provides nucleotide sequences that encode the CD40 binding molecules described herein.
The present invention also provides vectors that comprise nucleotide sequences that encode the CD40 binding molecules described herein. In some of such embodiments the vectors are expression vectors, for example comprising a nucleotide sequence that encodes a CD40 binding molecule operatively linked to a promoter.
Similarly, the present invention also provides cells (including human cells and murine cells) that comprise nucleotide sequences that encode the CD40 binding molecules described herein. Similarly, in some embodiments the present invention provides cells (including human cells and murine cells) that comprise nucleotide sequences that encode the CD40 binding molecules described herein operatively linked to a promoter and that express the CD40 binding molecules described herein.
The present invention also provides methods of use of the CD40 binding molecules, compositions, and cells described herein.
For example, in some embodiments the present invention provides methods for CD40-mediated activation of cells, such methods comprising contacting cells that express CD40 with a CD40 binding molecule as described herein (or a composition, such as a pharmaceutical composition, comprising such a CD40 binding molecule), thereby inducing intracellular signaling pathways. In some embodiments the present invention provides methods for activating cells expressing CD40, such methods comprising contacting cells that express CD40 with a CD40 binding molecule as described herein (or a composition, such as a pharmaceutical composition, comprising such a CD40 binding molecule), thereby activating CD40-induced signaling pathways in the cells. In some such embodiments the cells are myeloid cells. In some such embodiments the cells are lymphoid cells. In some such embodiments the cells are endothelial cells. In some such embodiments the cells are immune cells. In some such embodiments the cells are cells with innate or adaptive immune cell function. In some such embodiments the cells are antigen presenting cells. In some embodiments the antigen presenting cells are dendritic cells. In some embodiments the antigen presenting cells are B cells. In some embodiments the antigen presenting cells are macrophages. In some embodiments the cells are in vitro. In some embodiments the cells are in vivo.
In some embodiments the present invention provides methods of activating T cells via CD40-mediated activation of antigen presenting cells, such methods comprising contacting the antigen presenting cells with a CD40 binding molecule as described herein (or a composition, such as a pharmaceutical composition, comprising such a CD40 binding molecule). In some embodiments the antigen presenting cells are dendritic cells. In some embodiments the antigen presenting cells are B cells. In some embodiments the antigen presenting cells are macrophages. In some embodiments the antigen presenting cells are monocytes. In some embodiments the antigen presenting cells are endothelial cells. In some embodiments the cells are in vitro. In some embodiments the cells are in vivo.
In some embodiments the present invention provides methods for treating melanoma in subjects in need thereof, such methods comprising administering to a subject that has melanoma an effective amount of a CD40 binding molecule as described herein, (or a composition, such as a pharmaceutical composition, comprising such a CD40 binding molecule). In some such embodiments the method results in regression of a melanoma in the subject. In some such embodiments the method results in limiting proliferation of melanoma tumor cells in the subject. In some such embodiments the method results in killing of melanoma tumor cells in the subject. In some embodiments the survival of subjects treated with such methods is increased as compared to that of subjects with melanoma that are not so treated.
The present invention also provides methods for detecting CD40 or CD40-expressing cells in a sample (such as in a cell or tissue sample—e.g., a biopsy sample) or in a subject. Such methods typically involve contacting a cell or tissue sample with a CD40 binding molecule as described herein, and performing an assay to determine whether the CD40 binding molecule binds to the cell or tissue sample and/or performing an assay to quantify any binding of the CD40 binding molecule binds to the cell or tissue sample.
These and other embodiments of the invention are further described in the “Brief Description of the Figures,” “Detailed Description,” “Examples,” “Figures,” and “Claims” sections of this patent disclosure, each of which sections is intended to be read in conjunction with, and in the context of, all other sections of the present patent disclosure. Furthermore, one of skill in the art will recognize that the various embodiments of the present invention described herein can be combined in various ways, and that such combinations are within the scope of the present invention.
Some of the embodiments of the present invention are described in the “Summary of the Invention,” “Examples,” “Brief Description of the Figures,” and “Figures” sections of this patent disclosure. This Detailed Description section provides certain additional embodiments and certain additional description and details relating to embodiments described elsewhere herein, and is intended to be read in conjunction with all other sections of the present patent disclosure.
The practice of the present invention will employ, unless otherwise indicated, conventional techniques of cell biology, cell culture, molecular biology, transgenic biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See, e.g., Ausubel et al. eds. (2015) Current Protocols in Molecular Biology (John Wiley and Sons); Greenfield, ed. (2013) Antibodies: A Laboratory Manual (2nd ed., Cold Spring Harbor Press); Green and Sambrook, eds. (2012), Molecular Cloning: A Laboratory Manual (4th ed., Cold Spring Harbor Laboratory Press); Krebs et al., eds. (2012) Lewin's Genes XI (11th ed., Jones & Bartlett Learning); Freshney (2010) Culture Of Animal Cells (6th ed., Wiley); Weir and Blackwell, eds., (1996) Handbook Of Experimental Immunology, Volumes I-IV (5th ed., Wiley-Blackwell); Borrebaeck, ed. (1995) Antibody Engineering (2nd ed., Oxford Univ. Press); Glover and Hames, eds., (1995) DNA Cloning: A Practical Approach, Volumes I and II (2nd ed., IRL Press); Rees et al., eds. (1993) Protein Engineering: A Practical Approach (1st ed., IRL Press); Mayer and Walker, eds. (1987) Immunochemical Methods In Cell And Molecular Biology (Academic Press, London); Nisonoff (1984) Introduction to Molecular Immunology (2nd ed., Sinauer Associates, Inc.); and Steward (1984) Antibodies: Their Structure and Function (1st ed., Springer Netherlands).
In order that the present invention can be more readily understood, certain terms are defined herein. Additional definitions are set forth throughout the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention is related. For example, The Dictionary of Cell and Molecular Biology (5th ed. J. M. Lackie ed., 2013), the Oxford Dictionary of Biochemistry and Molecular Biology (2d ed. R. Cammack et al. eds., 2008), and The Concise Dictionary of Biomedicine and Molecular Biology (2d ed. P-S. Juo, 2002) can provide one of skill with general definitions of some terms used herein.
As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents, unless the context clearly dictates otherwise. The terms “a” (or “an”) as well as the terms “one or more” and “at least one” can be used interchangeably.
Furthermore, “and/or” is to be taken as specific disclosure of each of the two specified features or components with or without the other. Thus, the term “and/or” as used in a phrase such as “A and/or B” is intended to include A and B, A or B, A (alone), and B (alone).
Likewise, the term “and/or” as used in a phrase such as “A, B, and/or C” is intended to include A, B, and C; A, B, or C; A or B; A or C; B or C; A and B; A and C; B and C; A (alone); B (alone); and C (alone).
Units, prefixes, and symbols are denoted in their Systéme International de Unites (SI) accepted form. Numeric ranges provided herein are inclusive of the numbers defining the range.
Where a numeric term is preceded by “about” or “approximately,” the term includes the stated number and values±10% of the stated number.
Numbers in parentheses or superscript following text in this patent disclosure refer to the numbered references provided in the “Reference List” section of this patent disclosure.
Wherever embodiments are described with the language “comprising,” otherwise analogous embodiments described in terms of “consisting of” and/or “consisting essentially of” are included.
Amino acids are referred to herein by their commonly known three-letter symbols or by the one-letter symbols recommended by the IUPAC-IUB Biochemical Nomenclature Commission. Nucleotides, likewise, are referred to by their commonly accepted single-letter codes.
The abbreviation “CDR” refers to a complementary determining region—for example of an antibody or other CD40 binding molecule as described herein. Typically, the variable domains of both the heavy and light chains of antibodies comprise three hypervariable CDRs located within relatively conserved framework regions (FR). In general, the heavy and light chains of the variable domains of antibodies comprise, from N-terminal to C-terminal, FR1, CDR1, FR2, CDR2, FR3, CDR3 and FR4 regions. There are several different methodologies by which the assignment of amino acids in a variable domain to each of these FR and CDR regions can be performed. These include the “Kabat” method (described in Kabat, et al.; National Institutes of Health, Bethesda, Md.; 5.sup.th ed.; NIH Publ. No. 91-3242 (1991); Kabat (1978) Adv. Prot. Chem. 32:1-75; Kabat, et al., (1977) J. Biol. Chem. 252:6609-6616, the “Chothia” method (described by Chothia, et al., (1987) J Mol. Biol. 196:901-917 and Chothia, et al., (1989) Nature 342:878-883) and the “IMGT” ((ImMuno GeneTics) method (described by Lefranc et al., 2003, Dev Comp Immunol 27: 55-77). There can be slight differences in the boundaries of the various FR and CDR regions, and thus slight differences in the precise locations and amino acid sequences of the CDRs, depending on which of these methodologies is used.
The amino acid sequences described herein were analyzed using the IMGT methodology and the CDR sequences provided are those identified by the IMGT methodology. The IMGT (ImMunoGeneTics) numbering scheme is a standardized numbering system for all the protein sequences of the immunoglobulin superfamily, including variable domains from antibody light and heavy chains as well as T cell receptor chains from different species and counts residues continuously from 1 to 128 based on the germ-line V sequence alignment (see Giudicelli et al., Nucleic Acids Res. 25:206-11 (1997); Lefranc, Immunol Today 18:509 (1997); Lefranc et al., Dev Comp Immunol. 27:55-77 (2003)).
The term “humanized antibody” refers to an antibody derived from a non-human (e.g., murine) immunoglobulin, which has been engineered to contain minimal non-human (e.g., murine) sequences. Typically, humanized antibodies are human immunoglobulins in which residues from the complementary determining region (CDR) are replaced by residues from the CDR of a non-human species (e.g., mouse, rat, rabbit, or hamster) that have the desired specificity, affinity, and capability (Jones et al., 1986, Nature, 321:522-525; Riechmann et al., 1988, Nature, 332:323-327; Verhoeyen et al., 1988, Science, 239:1534-1536). In some instances, the Fv framework region (FW) residues of a human immunoglobulin are replaced with the corresponding residues in an antibody from a non-human species that has the desired specificity, affinity, and capability. The term “human antibody” means an antibody produced by a human or an antibody having an amino acid sequence corresponding to an antibody produced by a human made using any technique known in the art. The definition of a human antibody includes intact or full-length antibodies comprising at least one human heavy and/or light chain polypeptide such as, for example, an antibody comprising murine light chain and human heavy chain polypeptides.
The term “chimeric antibodies” refers to antibodies wherein the amino acid sequence of the immunoglobulin molecule is derived from two or more species. Typically, the variable region of both light and heavy chains corresponds to the variable region of antibodies derived from one species of mammals (e.g., mouse) with the desired specificity, affinity, and capability while the constant regions are homologous to the sequences in antibodies derived from another (usually human) to avoid eliciting an immune response in that species.
“Binding affinity” generally refers to the strength of the sum-total of non-covalent interactions between a single binding site of a molecule (e.g., a CD40 binding molecule) and its binding partner (e.g., CD40). Unless indicated otherwise, as used herein, “binding affinity” refers to intrinsic binding affinity which reflects a 1:1 interaction between members of a binding pair (e.g., a CD40 binding molecule and CD40). The affinity of a molecule X for its partner Y can generally be represented by the dissociation constant (KD). Affinity can be measured by common methods known in the art, including those described herein. Low-affinity antibodies generally bind antigen slowly and tend to dissociate readily, whereas high-affinity antibodies generally bind antigen faster and tend to remain bound longer. The affinity or avidity of an antibody for an antigen can be determined experimentally using any suitable method known in the art, e.g., flow cytometry, enzyme-linked immunosorbent assay (ELISA), or radioimmunoassay (RIA), or kinetics (e.g., KINEXA® or BIACORE™ or OCTET® analysis). Direct binding assays as well as competitive binding assay formats can be readily employed. (See, e.g., Berzofsky et al., “Antibody-Antigen Interactions,” In Fundamental Immunology, Paul, W. E., ed., Raven Press: New York, N.Y. (1984); Kuby, Immunology, W. H. Freeman and Company: New York, N.Y. (1992)). The measured affinity of a particular interaction (e.g., antibody-antigen interaction) can vary if measured under different conditions (e.g., salt concentration, pH, temperature). Thus, measurements of affinity and other antigen-binding parameters (e.g., KD or Kd, Kon, Koff) are made with standardized solutions of antibody and antigen, and a standardized buffer, as known in the art.
“Potency” is normally expressed as an IC50 or EC50 value, in nM or pM, unless otherwise stated. IC50 is the median inhibitory concentration of an antibody molecule. In functional assays, IC50 is the concentration that reduces a biological response by 50% of its maximum. In ligand-binding studies, IC50 is the concentration that reduces receptor binding by 50% of maximal specific binding level. EC50 is the median effective concentration of an antibody molecule. In functional assays, EC50 is the concentration that induces a biological response that is 50% of its maximum biological response. In ligand-binding studies, EC50 is the concentration that induces 50% of the maximal specific binding level. IC50 and EC50 values can be calculated by any number of means known in the art.
As used herein a “subject” is any individual for whom diagnosis, prognosis, or therapy is desired. In some embodiments the subjects are mammalian subjects, including humans, domestic animals, farm animals, sports animals, and zoo animals. In some embodiments the subjects are non-human primates. In some embodiments the subjects are murine subjects. In some embodiments the subjects are humans.
The term “pharmaceutical composition” refers to a preparation that is in such form as to permit the biological activity of the active agent (e.g., a CD40 binding molecule) to be effective and which contains no additional components that are unacceptably toxic to a subject to which the composition may be administered. Such compositions can be sterile.
Typically, such compositions comprise a pharmaceutically acceptable carrier. Examples of pharmaceutically acceptable carriers include, but are not limited to, physiological saline. In some embodiments pharmaceutical compositions can comprise one or more of: a buffer (e.g., acetate, phosphate, or citrate buffer), a surfactant (e.g., polysorbate), a stabilizing agent (e.g., human albumin), a preservative (e.g., benzyl alcohol), an absorption promoter to enhance bioavailability and/or other conventional solubilizing or dispersing agents.
The term “vector” means a construct for delivery of a nucleic acid molecule to a host cell. Examples of vectors include, but are not limited to, viruses, viral-derived vectors, naked DNA or RNA vectors, plasmid vectors, cosmid vectors, phage vectors, and the like. In some embodiments a vector may be an “expression vector” that is capable of delivering a nucleic acid molecule to a host cell and that also contains elements required for expression of the nucleic acid molecule in the host cell.
An “isolated” molecule is not within a living subject or cell and is typically in a form not found in nature. In some embodiments an isolated molecule may have been purified to a degree that it is not in a form in which it is found in nature. In some embodiments, a molecule that is isolated is substantially pure. In some embodiments, a molecule that is isolated has a purity of greater than 75%, or greater than 80%, or greater than 90%, or greater than 95%.
The terms “identical” or percent “identity” in the context of two or more nucleic acid sequences or amino acid sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of nucleotides or amino acid residues that are the same, when compared and aligned (introducing gaps, if necessary) for maximum correspondence, not considering any conservative amino acid substitutions as part of the sequence identity. The percent identity can be measured using sequence comparison software or algorithms or by visual inspection. Various algorithms and software are known in the art that can be used to obtain alignments of amino acid or nucleotide sequences.
Other terms are defined elsewhere in this patent disclosure, or else are used in accordance with their usual meaning in the art.
The acronym “CD40” refers to “Cluster of Differentiation 40.” CD40 is a costimulatory protein found on antigen-presenting cells that is required for their activation. The CD40 protein, and the nucleotide sequences that encode it, are well known in the art. The present invention provides CD40 binding molecules that specifically bind to and activate/agonize CD40.
The CD40 binding molecules of the present invention include, but are not limited to, those referred to herein as TDS-Y-003, Hu10F12 and Hu10FG2 (N103D)—each of which are humanized antibodies comprising a heavy chain and a light chain. The CD40 binding molecules of the present invention also include three different humanized variants of Hu10F12—each having identical CDRs but differences in their variable domain amino acid sequences outside of the CDR regions. Amino acid sequences comprising the variable regions of TDI-Y-003, three different humanized variants of Hu10F12, and Hu10F12 (N103D) are provided in Table A, below. The amino acid sequences provided in Table A were analyzed using the IMGT methodology, and the location and sequence of the CDR regions shown in Table A are those identified using the IMGT methodology.
QVQLQESGPGLVKPSETLSLTCTVS
GFSLTTYG
LHWI
RQPPGKGLEWIGV
IWRGGNT
DYNPSLKSRVTISKDTS
KNQVSLKLSSVTAADTAVYYC
AKNSYYG
Q
GGHAMD
Y
WGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAAL
DIVMTQSPDSLAVSLGERATINCKSS
QSLLHSFNQKN
Y
LAWYQQKPGQPPKLLIY
FTS
TRESGVPDRFSGSGS
GTDFTLTISSLQAEDVAVYYC
QQHYTTPFT
FGQGTKL
EIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPR
QVQLQESGPGLVKPSETLSLTCTVS
GFSLTTYG
LHWI
RQPPGKGLEWIGV
IWRGGNT
NYNPSLKSRVTISVDTS
KNQFSLKLSSVTAADTAVYYC
AKNSYYG
N
GGHAMD
Y
WGQGTLVTVSS
DIVMTQSPDSLAVSLGERATINCKSS
QSLLHSFNQKN
Y
LAWYQQKPGQPPKLLIY
FTS
TRESGVPDRFSGSGS
GTDFTLTISSLQAEDVAVYYC
QQHYTTPFT
FGQGTKL
EIK
QVQLQESGPGLVKPSETLSLTCTVS
GFSLTTYG
LHWI
RQPPGKGLEWIGV
IWRGGNT
DYNPSLKSRVTISKDTS
KNQVSLKLSSVTAADTAVYYC
AKNSYYG
N
GGHAMD
Y
WGQGTLVTVSS
DIVMTQSPDSLAVSLGERATINCKSS
QSLLHSFNQKN
Y
LAWYQQKPGQPPKLLIY
FTS
TRESGVPDRFLGSGS
GTDFTLTISSLQAEDVAVYFC
QQHYTTPFT
FGQGTKL
EIK
QVQLVESGGGVVQPGRSLRLSCAAS
GFSLTTYG
MH
WVRQAPGKGLEWVAV
IWRGGNT
DYNADSVKGRFTI
SRDNSKNTLYLQMNSLRAEDTAVYYC
AKNSYYG
N
G
GHAMDY
WGQGTLVTVSS
DIVMTQVPVSLAVSLGERATINCKSS
QSLLHSFNQKN
Y
LAWYQQKPGQSPKLLIY
FTS
TRESGVPDRFSGSGS
GTDFTLTISSLQAEDVAVYFC
QQHYTTPFT
FGQGTKL
EIK
QVQLKQSGPGLVQPSQSLSITCTVS
GFSLTTYG
LHW
VRQSPGKGLEWLGV
IWRGGNT
DYNAAFMSRLSITKD
NSKSQVFFKMNSLQADDTAIYYC
AKNSYYG
N
GGHA
MDY
WGQGTSVTVSS
DIVMTQSPSSLAMSVGQKVTMSCKSS
QSLLHSFNQK
NY
LAWYQQRPGQSPKLLIY
FTS
TRESGVPDRFLGSG
SGTDFTLTISSVQAEDLADYFC
QQHYTTPFT
FGSGTK
LEIK
In Table A, the amino acid sequences of the TDI-Y-003 heavy and light chains are presented in a human IgG2 format. However, one of skill in the art will recognize, as described elsewhere herein, that these sequences can be engineered to different immunoglobulin formats, and/or to produce antigen binding fragments and the like while retaining the key determinants for their CD40 binding and agonist activity—i.e., their CDRs. Similarly, in Table A the amino acid sequences of three different variants of the Hu10F12 heavy and light chain variable regions are presented. For these molecules, only the variable domain sequences are shown.
One of skill in the art will recognize that the specific full-length antibody sequences or full variable domain sequences provided herein can be engineered to contain various different constant regions and/or can be engineered to various different immunoglobulin formats, and/or can be engineered to produce antigen binding fragments and the like—as long as the molecules retain the key determinants for their CD40 binding and agonist activity—i.e., their CDRs.
Accordingly, in some embodiments the present invention provides CD40 binding molecules that comprise the CDR sequences of either TDI-Y-003, the 3 variants of Hu10F12 or Hu10F12 (N103D). The CDRs of these CD40 binding molecules are the same with the exception that they vary at the amino acid position in CDRH3 that corresponds to amino acid number 103 of SEQ ID NO. 1 (the position in CDRH3 that corresponds to amino acid number 103 of SEQ ID NO. can be readily determined by alignment of a given sequence to SEQ ID NO. 1 or to a portion of SEQ ID NO. 1). The amino acid sequences of the CDRs of TDI-Y-003, the 3 Hu10F12 variants and Hu10F12 (N103D), as determined using the IMGT methodology, are provided in Table B, below.
The red/italicized text in Table B represents the location of the amino acid in CDR H3 that corresponds to amino acid residue 103 of SEQ ID NO. 1. This amino acid is an N in hu10F12 and in SEQ ID NO. 11, a D in Hu10F12 (N103D) and in SEQ ID NO. 14, and a Q in TDI-Y-003 and in SEQ ID NO. 11. In SEQ ID NO. 16 this amino acid is A, R, N, D, E, Q, G, H, I, L, K, M, F, S, T, W, Y or V.
Accordingly, in some embodiments, the present invention provides CD40 binding molecules that comprise:
In some of such embodiments the CDR H3 domain in the CD40 binding molecule comprises SEQ ID NO. 11. In some such embodiments the CDR H3 domain in the CD40 binding molecule comprises SEQ ID NO. 14. In some such embodiments the CDR H3 domain in the CD40 binding molecule comprises SEQ ID NO. 15.
In some embodiments other amino acids are present at the position in CDR H3 that corresponds to amino acid residue 103 of SEQ ID NO. 1. In particular, in some embodiments this amino acid is A, R, N, D, E, Q, G, H, I, L, K, M, F, S, T, W, Y or V. Thus, in some embodiments the present invention provides CD40 binding molecules that comprise:
As described above, the locations/boundaries of the CDRs shown in Table A and Table B above were determined using the IMGT system. However, as also described above, there can be slight differences in the assignment of amino acids to particular FR or CDR region depending on the methodology used. The present invention encompasses CD40 binding molecules comprising the CDRs of the various molecules shown in Table A regardless of which system is used to identify the locations/boundaries of the CDRs. Accordingly, in some embodiments, the present invention provides CD40 binding molecules that comprise: (i) the CDR H1, CDR H2, and CDR H3 regions of SEQ ID NO. 1, 3, 5, 7, or 17; and (i) the CDR L1, CDR L2, and CDR L3 regions of SEQ ID NO. 2, 4, 6, 8, or 18.
For example, in some embodiments, the present invention provides a CD40 binding molecule that comprises: (i) the CDR H1, CDR H2, and CDR H3 regions of SEQ ID NO 1; and (i) the CDR L1, CDR L2, and CDR L3 regions of SEQ ID NO. 2. Similarly, in some embodiments, the present invention provides a CD40 binding molecule that comprises: (i) the CDR H1, CDR H2, and CDR H3 regions of SEQ ID NO 3; and (i) the CDR L1, CDR L2, and CDR L3 regions of SEQ ID NO. 4. In some embodiments, the present invention provides a CD40 binding molecule that comprises: (i) the CDR H1, CDR H2, and CDR H3 regions of SEQ ID NO 5; and (i) the CDR L1, CDR L2, and CDR L3 regions of SEQ ID NO. 6. In some embodiments, the present invention provides a CD40 binding molecule that comprises: (i) the CDR H1, CDR H2, and CDR H3 regions of SEQ ID NO 7; and (i) the CDR L1, CDR L2, and CDR L3 regions of SEQ ID NO. 8. In some embodiments, the present invention provides a CD40 binding molecule that comprises: (i) the CDR H1, CDR H2, and CDR H3 regions of SEQ ID NO 17; and (i) the CDR L1, CDR L2, and CDR L3 regions of SEQ ID NO. 18.
In some embodiments, in addition to the specific CD40 binding molecules (or portions thereof) whose sequences are provided in Table A, and in addition to CD40 binding molecules comprising the CDRs of the molecules whose sequences are provided in Table A, and in addition to CD40 binding molecules comprising the specific CDRs whose sequences are provided in Table B, the present invention also provides variant CD40 binding molecules (“variant molecules”). In some embodiments such variant molecules include humanized, chimeric, optimized, germ-lined, and/or other Ig-formatted versions of any of the specific CD40 binding molecules disclosed—herein provided that such variant molecules bind to, and are agonists of, human CD40. Similarly, in some embodiments such variant molecules include molecules that comprise variants of the specific CDR sequences disclosed herein —provided that such variant molecules bind to, and are agonists of, human CD40. For example, in some embodiments variant molecules that comprise a CDR that has 85%, 90%, 95%, 96%, 97%, 98% or 99% amino acid identity to a CDR sequence as set forth herein are included —provided that such variant molecules bind to, and are agonists of, human CD40. Similarly, in some embodiments variant molecules that comprise a CDR that has 1, or 2, or 3 amino acid substitutions, additions, or deletions as compared to a CDR sequence as set forth herein are included—provided that such variant molecules bind to, and are agonists of, human CD40. In some of such embodiments such amino acid substitutions are conservative substitutions.
Similarly, in some embodiments the present invention provides variant molecules that comprise a VH and/or VL amino acid sequence that is at least 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% identical to one of the specific VH or VL sequences provided herein (e.g., as provided by, or comprised within, one of SEQ ID NO. 1 to SEQ ID NO. 8) and/or that comprises 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more amino acid substitutions (e.g., conservative substitutions), additions or deletions relative to one of the specific VH or VL sequences provided herein (e.g., as provided by, or comprised within, one of SEQ ID NO. 1 to SEQ ID NO. 8)—provided that such variant molecules bind to, and are agonists of, human CD40. In some such embodiments, the amino acid differences (e.g., substitutions, additions, deletions, etc.) are outside of the CDRs.
In some embodiments, a CD40 binding molecule or variant CD40 binding molecule as described herein, in addition to binding to, and being an agonist of human CD40, also has one or more the following properties: (a) binding to the epitope on CD40 that is bound by TDI-Y-003, Hu10F12 or Hu10F12 (N103D), (b) competition with TDI-Y-003 or Hu10F12 or Hu10F12 (N103D) for binding to its epitope on CD40, (c) CD40 agonist activity without a requirement for Fc receptor engagement, and (d) APC activation activity (e.g., dendritic cell activation activity).
In some embodiments, a CD40 binding molecule according to the present invention is a murine antibody, a human antibody, a humanized antibody, a chimeric antibody, a monoclonal antibody, a polyclonal antibody, a bi-specific antibody, or a multi-specific antibody.
In some embodiments a CD40 binding molecule according to the present invention is, or comprises, a Fab, a Fab′, a F(ab′)2, a Fd, a Fv, a scFv, a disulfide linked Fv, a V-NAR domain, an IgNar, a VHH domain, a nanobody, an intrabody, an IgGΔCH2, a minibody, a F(ab′)3 a tetrabody, a triabody, a diabody, a single-domain antibody, DVD-Ig, Fcab, mAb2, a (scFv)2, or a scFv-Fc.
In some embodiments, a CD40 binding molecule according to the present invention comprises a heavy chain variable domain (VH). In some embodiments, a CD40 binding molecule according to the present invention comprises a light chain variable domain (VL). In some embodiments, a CD40 binding molecule according to the present invention comprises a heavy chain variable domain (VH) and a light chain variable domain (VL). In some embodiments, a CD40 binding molecule according to the present invention comprises a heavy chain constant region (CH). In some embodiments, a CD40 binding molecule according to the present invention comprises a light chain constant region (CL). In those embodiments where the CD40 binding molecule comprises a constant region, in some of such embodiments the constant region is a human constant region. In some of such embodiments the human constant region is a human IgG constant region. In some of such embodiments the human constant region is a human IgG2 constant region.
In some embodiments the present invention provides CD40 binding molecules that can specifically bind to the same CD40 epitope as is bound by TDI-Y-003, Hu10F12 or Hu10F12 (N103D). Similarly, in some embodiments the present invention provides CD40 binding molecules that compete for binding to CD40 with TDI-Y-003 or Hu10F12 Hu10F12 (N103D).
In some embodiments, the CD40 binding molecules provided herein bind to human CD40 with a binding affinity characterized by a dissociation constant (KD) of about 100 pM to about 50 nM. In some embodiments, the CD40 binding molecules provided herein bind to human CD40 with a binding affinity characterized by a dissociation constant (KD) of about 10 nM to about 50 nM. In some embodiments, the CD40 binding molecules provided herein bind to human CD40 with a binding avidity characterized by a dissociation constant (KD) of about 1 nM to about 2 nM. In some embodiments such binding affinities or avidities are determined using a Biacore™ assay or on a Kinetic Exclusion Assay (KinExA®) 3000 platform or on an Octet® instrument.
In some embodiments, the CD40 binding molecules provided herein specifically bind to human CD40 with a dissociation constant or KD of less than 10−6 M, or of less than 10−7 M, or of less than 10−8 M, or of less than 10−9 M, or of less than 10−10 M, or of less than 10−11 M, of less than 10−12 M, of less than 10−13 M, of less than 10−14 M, or of less than 10−15 M as measured, e.g., by Biacore™ or KinExA® or Octet®.
In some embodiments, the CD40 binding molecules provided herein bind to human CD40 with a Koffof less than 1×10−3 s−1, or less than 2×10−3 s−1. In other embodiments, the CD40 binding molecules provided herein bind to human CD40 with a Koff of less than 10−3 s−1, less than 5×10−3 s−1, less than 10−4 s−1, less than 5×10−4 s−1, less than 10−5 s−1, less than 5×10−5 s−1, less than 10−6 s−1, less than 5×10−6 s−1, less than less than 5×10−7 s−1, less than 10−8 s−1, less than 5×10−8 s−1, less than 10−9 s−1, less than 5×10−9 s−1, or less than 101 s−1 as measured, e.g., by Biacore™ or KinExA® or Octet®.
In some embodiments, the CD40 binding molecules provided herein bind to human CD40 with an association rate constant or Kon rate of at least 105 M−1 s−1, at least 5×105 M−1 s−1, at least 106 M−1 s−1 at least 5×106 M−1 s−1, at least 107 M−1 s−1 at least 5×107 M−1 s−1, or at least 108 M−1 s−1, or at least 109 M−1 s−1 as measured, e.g., by Biacore™ or KinExA® or Octet®.
The CD40 binding molecules of the invention can be prepared using any suitable means known in the art. For example, the CD40 binding molecules of the invention can be prepared using recombinant DNA methods. For example, polynucleotides encoding the CD40 binding molecules can be cloned into suitable expression vectors. Transfection of host cells with the expression vector results in generation of the molecules by the host cells.
The present invention provides various methods of using the CD40 binding molecules described herein.
For example, in some embodiments the present invention provides methods for activating cells expressing CD40, such methods comprising contacting cells that express CD40 with a CD40 binding molecule as described herein (or a composition, such as a pharmaceutical composition, comprising such a CD40 binding molecule), thereby activating CD40-induced signaling pathways in the cells. In some embodiments the present invention provides methods for activating cells expressing CD40, such methods comprising contacting cells that express CD40 with a CD40 binding molecule as described herein (or a composition, such as a pharmaceutical composition, comprising such a CD40 binding molecule), thereby activating CD40-induced signaling pathways in the cells. In some such embodiments the cells are myeloid cells. In some such embodiments the cells are lymphoid cells. In some such embodiments the cells are endothelial cells. In some such embodiments the cells are immune cells. In some such embodiments the cells are cells with innate or adaptive immune cell function. In some such embodiments the cells are antigen presenting cells. In some embodiments the antigen presenting cells are dendritic cells. In some embodiments the antigen presenting cells are B cells. In some embodiments the antigen presenting cells are macrophages. In some embodiments the cells are in vitro. In some embodiments the cells are in vivo.
The present invention provides methods of activating T cells via CD40-mediated activation of antigen presenting cells, such methods comprising contacting the antigen presenting cells with a CD40 binding molecule as described herein (or a composition, such as a pharmaceutical composition, comprising such a CD40 binding molecule). In some embodiments the antigen presenting cells are dendritic cells. In some embodiments the antigen presenting cells are B cells. In some embodiments the antigen presenting cells are macrophages. In some embodiments the antigen presenting cells are monocytes. In some embodiments the antigen presenting cells are endothelial cells. In some embodiments the cells are in vitro. In some embodiments the cells are in vivo.
The present invention also provides methods for detecting CD40 or CD40-expressing cells in a sample (such as in a cell or tissue sample—e.g., a biopsy sample) or in a subject. Such methods typically involve contacting a cell or tissue sample with a CD40 binding molecule as described herein, and performing an assay to determine whether the CD40 binding molecule binds to the cell or tissue sample and/or performing an assay to quantify any binding of the CD40 binding molecule binds to the cell or tissue sample.
In some embodiments the present invention provides methods for treating melanoma in subjects in need thereof, such methods comprising administering to a subject that has melanoma an effective amount of a CD40 binding molecule as described herein, (or a composition, such as a pharmaceutical composition, comprising such a CD40 binding molecule). In some such embodiments the method results in regression of a melanoma in the subject. In some such embodiments the method results in a delay of tumor growth or a reduction in the rate of tumor growth. In some such embodiments the method results in limiting proliferation of melanoma tumor cells in the subject. In some such embodiments the method results in killing of melanoma tumor cells in the subject. In some embodiments the survival of subjects treated with such methods is increased as compared to that of subjects with melanoma that are not so treated.
As used herein, the terms “treat,” “treating,” “treatment,” “administration,” and “administering” refer to contact of an exogenous pharmaceutical, therapeutic, or composition to an animal, human, subject, cell, tissue, organ, or biological fluid. Treatment of a cell encompasses contact of a reagent to the cell, as well as contact of a reagent to a fluid, where the fluid is in contact with the cell. “Administration” and “treatment” also means in vitro and ex vivo treatments, e.g., of a cell, by a reagent, binding compound, or by another cell.
In carrying out the treatment methods described herein, any suitable method or route of administration can be used to deliver the CD40 binding molecules described herein. In some embodiments systemic administration may be employed, for example, intravenous administration, or any other route of systemic administration known in the art that is suitable for use with the present CD40 binding molecules. In some embodiments intra-tumoral delivery may be employed. For example, the active agents described herein may be administered directly into a tumor by local injection, infusion through a catheter placed into the tumor, delivery using an implantable drug delivery device inserted into a tumor, or any other means known in the art for direct delivery of an agent to a tumor.
As used herein the term “effective amount” refers to an amount of a CD40 binding molecule (or a composition comprising a CD40 binding molecule) as described herein that, when administered alone or in combination with an additional therapeutic/prophylactic agent to a cell, tissue, or subject, is effective to prevent or cause a measurable improvement in one or more symptoms of the condition being treated (e.g., melanoma). For example, such measurable improvements include, but are not limited to, reducing the rate of growth of a tumor (or of tumor cells), halting the growth of a tumor (or of tumor cells), causing regression of a tumor (or of tumor cells), reducing the size of a tumor (for example as measured in terms of tumor volume or tumor mass), reducing the grade of a tumor, eliminating a tumor (or tumor cells), and the like. When applied to a combination, an effective dose refers to combined amounts of the active ingredients that result in the prophylactic or therapeutic effect, whether administered in combination, serially or simultaneously.
An appropriate “effective” amount in any individual case may be determined using standard techniques known in the art, such as dose escalation studies, and may be determined taking into account such factors as the desired route of administration (e.g., systemic vs. intra-tumoral), desired frequency of dosing, etc. Furthermore, an “effective amount” may be determined in the context of any co-administration method to be used. One of skill in the art can readily perform such dosing studies (whether using single agents or combinations of agents) to determine appropriate doses to use, for example using assays such as those described in the Examples section of this patent application—which involve administration of CD40 binding molecules to animal subjects routinely used in the pharmaceutical sciences for performing dosing studies.
In certain embodiments the compositions and methods of treatment provided herein may be employed together with other compositions and treatment methods known to be useful for the given condition being treated (here—melanoma), including, but not limited to, surgical methods (e.g., melanoma resection), radiation therapy methods, treatment with chemotherapeutic agents, treatment with immune checkpoint inhibitors, and the like. Similarly, in certain embodiments the methods of treatment provided herein may be employed together with procedures used to monitor disease status/progression, such as biopsy methods and diagnostic methods (e.g., MRI methods or other imaging methods).
The invention is further described by, and understood with reference to, the following non-limiting Examples, as well as the Figures referred to therein.
Background
Stimulation of the CD40 pathway in APCs has been recognized as a highly effective way to activate APCs.6,7 CD40 agonists have demonstrated antitumor activity as monotherapy in human cancers.8-10 Currently there is great interest in the use of CD40 stimulation in the treatment of pancreatic cancer, in part because of compelling early-phase data incorporating the CD40 agonist mAb APX005M (Apexigen) along with chemotherapy and PD-1 blockade in the first-line treatment of metastatic pancreatic cancer.11
Several approaches to induce CD40 signaling with an agonist agent have been explored.20 For example, certain CD40 agonist antibodies with a modified Fc portion that increases binding to FcγRIIB, which is present on intra-tumoral myeloid cells, can more potently stimulate APCs.13 This has led to the production of a series of CD40 agonist antibodies designed to have increased FcγRIIB binding, including APX005M14 or 2141-V1115.
Instead of relying on Fc receptor engagement, this approach focused on identifying CD40 agonist antibodies with certain other intrinsic features, including competition with the binding site of CD40L, the ligand for CD40. Binding CD40 at the natural ligand binding site has been shown to increase agonist activity,22 and may further reduce inter-patient variability by preventing uncontrolled activation of CD40 by endogenous CD40L.
Identification & Characterization of CD40 Binding Molecules
Antibody generation was carried out by immunizing mice with recombinant CD40 protein. Hybridomas were generated by electrofusion, and the supernatants were screened for antibodies that bound to CD40, competed with CD40L for binding to CD40, and displayed potent agonist activity on cells that overexpress CD40. After completion of subcloning and sequencing, chimeric versions of the top-ranked agonist antibodies were produced with the murine variable regions fused to human constant regions with either an IgG1 or IgG2 isotype subclass. The human/mouse chimeric antibodies were tested along with the parental murine antibodies in an APC activation assay using dendritic cells isolated from PBMCs.
The 10F12 antibody was selected as a lead candidate based on it having the following biological and functional properties:
For immunizations, the extracellular domains of human CD40 (residues 21-192), cynomolgus monkey CD40 (residues 21-192) and mouse CD40 (residues 24-192), each fused via their carboxy-terminus to a human Fc fragment, were used.
Two cohorts of mice (Balb/c and NZB/W strains) were immunized with human CD40-Fc. For another cohort of Balb/c mice, human, cynomolgus monkey and mouse CD40-Fc were used alternatively in consecutive immunizations, with the goal to obtain antibodies that are cross-reactive between human, cynomolgus and mouse CD40. Serum was collected to assess the immune response to CD40. All animals had midpoint titers of 1; 10,000 or greater when analyzed by ELISA using recombinant human CD40 (residues 21-192) fused to a 6×His-tag (SEQ ID NO. 19) at the carboxy-terminus. Weak binding to mouse CD40-His at mid-point titers of 1:200 or lower was observed for sera from the Balb/c mice that had been immunized with CD40-Fc from various species. Three days after the final boost, lymph nodes were collected and pooled for each cohort, followed by isolation of IgG-producing B cells by magnetic sorting. The enriched B cells were fused with mouse myeloma cells to generate hybridomas, which were plated for screening.
Hybridoma supernatants were analyzed. Binding to human, cynomolgus monkey and mouse CD40 was analyzed by ELISA, followed by testing of the binders for interaction with a HEK293 cell line stably expressing human CD40 on the surface.
Subsequently, competition of the antibodies with human CD40L for binding to CD40 was analyzed by ELISA, and agonist activity was assessed using a HEK293 cell line that overexpresses human CD40 and includes a luciferase reporter gene under the NF-kB promoter.
To assess the contribution of the Fc portion to the agonist activity of the CD40 lead candidate antibodies, they were re-formatted to generate chimeric molecules with mouse variable regions fused to human constant regions of either the IgG1 or IgG2 isotype subclass. The chimeric mAbs were expressed recombinantly in HEK293 cells to obtain purified material that can be used to determine dissociation constants in solution, agonist potency on cells, and activity in an APC stimulation assay.
Dissociation constants (KD), on-rates (kon) and off-rates (koff) for the 10F12 chimeric mAb were determined by BLI using soluble, monomeric human CD40-His (Table 1). The KD value for binding to human CD40 in solution was 27.1 nM and 26.5 nM for human IgG1 and human IgG2, respectively.
Agonist activity was measured using the HEK293-CD40 NF-kB luciferase reporter cell line in the presence of anti-human Fc to mediate antibody crosslinking. Table 2 summarizes the EC50 values and maximum stimulation compared to CD40L.
The chimeric CD40 antibodies were tested in a human dendritic cell activation assay. Monocytes were first isolated from healthy human donor blood by Ficoll density gradient centrifugation and plastic adherence. To obtain monocyte-derived dendritic cells, adherent cells were cultured in the presence of hIL-4 and hGM-CSF for five to seven days. Differentiated dendritic cells (DCs) were harvested and plated in 96 wells at a concentration of 0.5-2×106/mL and stimulated for 48 hours with 10 μg/mL anti-CD40 antibodies. A maturation mix of prostaglandin E, TNFα, IL-1b, IL-4, IL-6, and GM-CSF or the Toll-like receptor 4 (TLR4) agonists lipopolysaccharide (LPS) and monophosphoryl lipid A (MPL) were used as positive controls to activate DCs. Cell culture supernatants were analyzed for cytokine secretion in multiplexed immunoassays. Dendritic cells were washed and subjected to immunostaining, followed by flow cytometry analysis. The following DC activation markers were analyzed: HLAABC, HLA-DR, CD80, and CD86.
Antibody 10-F12A formatted with a human IgG2 constant region (10-F12 hIgG2) emerged as a robust agonist in a series of dendritic cell activation assays with peripheral blood mononuclear cells (PBMCs) from multiple donors (
Humanization of the 10F12 Antibody
Humanization of the murine 10F12 antibody variable region was carried out by homology modeling followed by assessment of the productivity and antigen binding properties of a panel of candidate molecules. The selected humanized candidate antibody sequence was then analyzed in silico for potential post-translational modification sites in the CDRs and for potential predicted immunogenicity.
Analysis of the humanized mAb 10F12 variable region sequence identified a potential deamidation ‘hot-spot’ (an asparagine followed by a glycine, NG) in CDR3 of the heavy chain. To evaluate the impact of deamidation on the binding properties of the humanized 10F12 antibody, a charge mimic mutation (N103D) was introduced. The charge mimic variant, mAb hu10F12 (N103D), retained the specificity and affinity of the parent antibody hu10F12. An N103Q mutation was introduced in the hu10F12 mAb in order to ablate the CDR3 deamidation site, generating mAb hu10F12 (N103Q). The introduction of the conservative N to Q change had minimal impact on the biopharmaceutical, binding, and functional properties. Hu10F12 (N103Q) was selected as the lead mAb and designated TDI-Y-003.
Antibody TDI-Y-003 produced in stable CHO pools was tested in a series of binding and functional assays in vitro as well as an in vivo study in a disease-relevant animal model. Affinity to human CD40 was measured by BLI using an Octet Red96e. TDI-Y-003 binds to CD40 with a KD of 40 nM, with an intermediate on-rate and a relatively fast off-rate (Table 3). The KD of TDI-Y-003 for binding to human CD40-Fc was 1.2 nM. The slower off-rate seen for this interaction is likely a result of the avidity between the bivalent TDI-Y-003 IgG and the dimeric CD40-Fc.
Affinity to cynomolgus monkey and mouse CD40 was measured by BLI using an Octet Red96e. TDI-Y-003 binds to cyno CD40 with a KD of 48 nM (Table 4), which is similar to human CD40 (40 nM). TDI-Y-003 showed no binding to mouse CD40-His at concentrations up to 1.7 μM.
To assess the binding of TDI-Y-003 to cell surface-bound CD40, HEK293 overexpressing CD40 as well as Raji cells (B-lymphocytes) and primary human dendritic cells, which express endogenous CD40, were used. TDI-Y-003 bound HEK293-CD40 and Raji cells with EC50 values in the sub-nanomolar range at 0.44 and 0.77 nM, respectively (Table 5). The EC50 value for binding to dendritic cells was 3.85 nM.
Competitive binding of TDI-Y-003 with CD40L for binding to CD40 was assessed using HEK293 cells stably expressing CD40. Cells were co-incubated with HA tagged CD40 ligand and TDI-Y-003 or isotype control at the indicated concentrations and analyzed for CD40 ligand binding by flow cytometry (
TDI-Y-003 was tested for agonist activity using a HEK293 cell line stably expressing CD40 with an NF-kB-luciferase reporter. To assess the contribution of the Fc portion, a F(ab′)2 fragment of TDI-Y-003 was included. In addition, CD40 ligand and a Fab fragment of TDI-Y-003 were utilized controls.
Human dendritic cells were used to analyze the ability of the TDI-Y-003 antibody to activate professional antigen presenting cells. Monocytes were isolated from human blood and differentiated into dendritic cells as previously described and as shown in
The DC activation assay showed a strong increase in expression of all dendritic cell activation markers tested. Expression levels of HLA-ABC, HLA-DR, CD80, and CD86 increased in a dose-dependent manner and to comparable extents in four different donors. Efficacy of TDI-Y-003 reached a plateau at a concentration of 5 μg/mL in all individually tested donors. Treatment with a human IgG2 isotype control did not result in detectable DC activation. Average EC50 values were calculated (Table 8) using dose-response curves from four different donors (
Differentiated, matured dendritic cells and other antigen presenting cells stimulated with TDI-Y-003 were also assessed for cytokine secretion. Secretion of the following cytokines were analyzed: IL-12p40, IL-10, IL-8, MIP-1α, and TNFα (
The DC activation assay was also used to analyze the impact of abrogating the TDI-Y-003 antibody's ability to bind to the Fc receptor on the efficacy of the TDI-Y-003 antibody on activating dendritic cells. Differentiated and matured dendritic cells were stimulated for 48 hours with TDI-Y-003 (full version), a variant of TDI-Y-003 that includes Fc mutations that abrogate Fcγ receptor binding (“TDI-Y-003 hIgG2d”), a variant of TDI-Y-003 that is a bivalent pepsin-digested IgG with no Fc portion (“TDI-Y-003 F(ab)2”), a variant of TDI-Y-003 that is a monovalent pepsin-digested IgG with no Fc portion (“TDI-Y-003 F(ab)”), or human IgG2 isotype control, at concentrations ranging from 0.001 μg/mL to 100 μg/mL. A maturation cocktail, LPS, or MPL were used as positive controls to activate DCs. CD agonists CP-870893 (Pfizer) or selicrelumab (Roche) (“Ab1 hIgG2”), APX-005 (Apexigen) (“Ab2 hIgG1 S267E”), and dacetuzumab (Seattle-Genetics) (“Ab3-hIgG1”) were also included as additional controls. DC activation markers HLA-ABC and CD80 were analyzed (
As shown in
Additionally, the DC activation assay was used to compare the ability to activate dendritic cells of TDI-Y-003 with other CD40 agonists. In one study, differentiated and matured dendritic cells were stimulated for 48 hours with TDI-Y-003, CD40 agonist B016-Ab1 (equivalent to selicrelumab), or human IgG2 isotype control at concentrations ranging from 0.01 μg/mL to 5 μg/mL. A maturation cocktail and LPS were used as positive controls to activate DCs. As shown in
In another study, differentiated and matured dendritic cells were stimulated for 48 hours with TDI-Y-003, CD40 agonist APX005M, CD40 agonist CDX-1140, or human IgG2 isotype control at concentrations of 0.1 μg/mL and 10 μg/mL. A maturation cocktail, LPS, or MPL were used as positive controls to activate DCs. As shown in
To expand functional in vitro analyses of TDI-Y-003 efficacy to another antigen-presenting cell type, B cells were tested (
TDI-Y-003 efficacy was also studied on primary human B cells. The B cells were stimulated with TDI-Y-003 at concentrations ranging from 0.01 μg/mL to 50 μg/mL. After 48 hours cells were washed, stained, and subjected to flow cytometry analysis to study activation marker expression and cytokine secretion. CpG ODN was included as a positive control to activate B cells, and ctrl ODN was the corresponding control oligonucleotide. The following B cell activation markers were analyzed: HLA-ABC, HLA-DR, CD80, CD86, and CD70 (
T cells were used to analyze whether stimulation of antigen presenting cells by TDI-Y-003 can lead to activation of T cells (
The in vivo efficacy of TDI-Y-003 was assessed using syngeneic B16F10 melanoma (known to be resistant to PD-1 and CTLA-4 blocakde18,19) in C57BL/6 transgenic mice engineered to express human CD40 and human FcγRs17. These mice recapitulate normal antibody pharmacokinetics as they include the human version of FcRn. These mice also contain a human IgG1 knock-in tolerizing them to human IgGs, allowing for repeated human antibody administration without excess risk of immunogenicity from exogenously administered human IgG antibodies.
Bilateral B16-F10 melanoma tumors were implanted in hCD40/hFcγRs mice by injecting 1×106 cells intradermally in two flanks in each mouse (
Administration of TDI-Y-003 led to regression of injected tumors and delay of distant (nontreated) tumors, resulting in a significant survival benefit of the humanized mice with bilateral tumors (
TDI-Y-003 resulted in greater tumor control as a monotherapy than our initial experiments using an anti-mouse CD40 antibody (FGK45) suggested12. The in vivo studies with TDI-Y-003 indicate that the antibody may confer clinical benefit as monotherapy.
In addition, the impact of abrogating TDI-Y-003's ability to bind to the Fc receptor on the in vivo efficacy of TDI-Y-003 was assessed. Bilateral B16-F10 melanoma tumors were implanted in hCD40/hFcγRs mice by injecting 1×106 cells intradermally in two flanks in each mouse. After the tumors were established, the mice were injected with TDI-Y-300 (20 μg), TDI-Y-003 hIgG2σ (which has Fc mutations that abrogate Fcγ receptor binding) (20 μg), or an isotype control intratumorally twice weekly for four weeks. Administration of TDI-Y-003 and TDI-Y-003 hIgG2σ each led to significant regression of injected tumors and to delay of distant tumors (
Finally, a clonogenic assay was performed on in C57BL/6 mice having bilateral B16-F10 melanoma tumors as described above. Human IgG2 isotype control or TDI-Y-003 were injected into the established tumors at day 8 after tumor implantation. The tumors were then harvested within 24 hours and CD45+ cells were separated. The results show that there were significantly fewer tumor cell colonies from tumors injected with TDI-Y-003 (
Nat Rev Cancer 16, 447-462 (2016).
This application claims benefit of U.S. Provisional Application No. 63/126,282, filed Dec. 16, 2020, the entirety of which is herein incorporated by reference.
This invention was made with government support under grant number CA008748 awarded by the National Institutes of Health. The government has certain rights in the invention.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2021/063870 | 12/16/2021 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 63126282 | Dec 2020 | US |
