Claims
- 1. A cell culture system which comprises living eucaryotic cells of human or animal origin, or mycophyta, dispersed within a porous carrier bed, said carrier bed comprising particulate material, and a support means for said carrier bed, said particulate material providing sufficient internal cavities or spaces for retaining and permitting said cells to grow and multiply within said carrier bed, and said support means allowing liquid media to pass through and out of said carrier bed and to have contact with said cells.
- 2. A cell culture system according to claim 1 wherein the particle retention size of said carrier bed is from 0.1 to 2.00 .mu.m.
- 3. A cell culture system according to claim 1 wherein said particulate material is a diatomaceous earth.
- 4. A cell culture system according to claim 1 wherein said diatomaceous earth is selected from the class consisting of diatomite and kieselguhr.
- 5. A cell culture system according to claim 1 wherein said carrier bed comprises more than one horizontal layer of particulate material.
- 6. A cell culture system according to claim 5 wherein the particle retention size of said layers of particulate material generally decreases from the top surface layer to the layer adjacent the bottom surface layer.
- 7. A cell culture system according to claim 6 wherein the particle retention size of said layers is from 0.1 to 0.75 .mu.m.
- 8. A cell culture system according to claim 6 wherein the particle retention size of said layers is from 0.2 to 0.5 .mu.m.
- 9. A cell culture system according to claim 6 wherein said bottom surface layer is of greater particle retention size than the layer adjacent thereto.
- 10. A cell culture system according to claim 9 wherein the particle retention size of said bottom surface layer is about 0.5 .mu.m.
- 11. A cell culture system according to claim 1 wherein said carrier bed comprises a single layer of particle retention size 0.75 .mu.m to 2.0 .mu.m.
- 12. A cell culture system according to claim 11 wherein the particle retention size is 1.2 .mu.m.
- 13. A cell culture system according to claim 1 wherein the thickness of a layer of said carrier bed is from 8 mm to 20 mm.
- 14. A cell culture system according to claim 1 wherein said eucaryotic cells are selected from the class consisting of baby hamster clone 21 cell line and IB2 Renal Swine pig kidney cell line.
- 15. A cell culture system according to claim 1 wherein said cells are infected with a microorganism.
- 16. A cell culture system according to claim 15 wherein said microorganism is a virus.
- 17. A cell culture system according to claim 16 wherein said virus is a strain of foot-&-mouth disease virus.
- 18. A cell culture system according to claim 1 wherein said carrier bed is supported on a horizontal plate pressure filter.
- 19. A cell culture system according to claim 1 wherein said carrier bed is supported on a porous support means.
- 20. A cell culture system according to claim 1 wherein said carrier bed is supported on a support means having means for allowing said liquid media to pass through and out of said carrier bed.
- 21. A recirculating cell culture system which comprises living eucaryotic cells of human or animal origin, or mycophyta, dispersed within a porous carrier bed, said carrier bed comprising particulate material, said particulate material providing sufficient internal cavities or spaces for retaining and permitting said cells to grow and multiply within said carrier bed, a support means for said carrier bed, said support means allowing liquid media to pass through and out of said carrier bed and to have contact with said cells, and means for recirculating said liquid media through said carrier bed.
Priority Claims (1)
Number |
Date |
Country |
Kind |
19387/72 |
Apr 1972 |
GBX |
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Parent Case Info
This application is a continuation in part of Ser. No. 670,448, filed Mar. 25, 1976 now U.S. Pat. No. 4,085,203 and Ser. No. 354,195, filed Apr. 25, 1973, now abandoned.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
3493651 |
Sloane |
Feb 1970 |
|
3717551 |
Bizzini et al. |
Feb 1973 |
|
4055466 |
Torney et al. |
Oct 1977 |
|
Foreign Referenced Citations (4)
Number |
Date |
Country |
1517758 |
Jan 1970 |
DEX |
1598245 |
Aug 1970 |
FRX |
786824 |
Nov 1957 |
GBX |
989247 |
Apr 1965 |
GBX |
Related Publications (1)
|
Number |
Date |
Country |
|
354195 |
Apr 1973 |
|
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
670448 |
Mar 1976 |
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