Claims
- 1. The method of preparing a composition of matter having activity as an inhibitor of cell proliferation .Iadd.and tissue invasion .Iaddend.comprising the steps of providing connective tissue having a high content of collagen or proteoglycans in condition for extraction, extracting said inhibitor from said tissue with an aqueous extraction medium which includes a solute which does not irreversibly denature the proteinaceous matter to be extracted, separating the resultant aqueous extract from the tissue, recovering from the aqueous extract substances having a molecular weight below about 50,000, treating the fraction of aqueous extract having a molecular weight below about 50,000 to remove salts therefrom, and dehydrating the resultant material.
- 2. The method of claim 1 wherein the connective tissue comprises tissue normally devoid of an intimate capillary blood supply.
- 3. The method of claim 2 wherein the tissue comprises cartilage.
- 4. The method of claim 2 wherein the tissue comprises blood vessels.
- 5. A composition of matter having activity as an inhibitor of cell proliferation .Iadd.and tissue invasion .Iaddend.comprising the product of the method of claim 1.
- 6. A composition of matter having activity as an inhibitor of cell proliferation comprising the product of the method of claim 2.
- 7. A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 1.
- 8. A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 2.
- 9. A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 3.
- 10. A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 4.
Government Interests
The invention described herein was made in the course of work under a grant or award from the Department of Health, Education and Welfare.
Non-Patent Literature Citations (1)
Entry |
Willmer-Cells and Tissues in Culture, vol. 1 (1965), pp. 44 and 573. |
Reissues (1)
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Number |
Date |
Country |
Parent |
630275 |
Nov 1975 |
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