Claims
- 1. An isolated polynucleotide comprising:
(a) a nucleotide sequence encoding a polypeptide having chalcone synthase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2 or 4 have at least 80% sequence identity, or (b) the full-length complement of the nucleotide sequence of (a).
- 2. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2 or 4 have at least 85% identity.
- 3. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2 or 4 have at least 90% identity.
- 4. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2 or 4 have at least 95%.
- 5. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide comprises the amino acid sequence of SEQ ID NO:2 or 4.
- 6. The polynucleotide of claim 1 wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:1 or 3.
- 7. A vector comprising the polynucleotide of claim 1.
- 8. A recombinant DNA construct comprising the polynucleotide of claim 1 operably linked to at least one regulatory sequence.
- 9. A method for transforming a cell, comprising transforming a cell with the polynucleotide of claim 1.
- 10. A cell comprising the recombinant DNA construct of claim 8.
- 11. A method for producing a plant comprising transforming a plant cell with the polynucleotide of claim 1 and regenerating a plant from the transformed plant cell.
- 12. A plant comprising the recombinant DNA construct of claim 8.
- 13. A seed comprising the recombinant DNA construct of claim 8.
- 14. An isolated polynucleotide comprising a first nucleotide sequence, wherein the first nucleotide sequence contains at least 30 nucleotides, and wherein the first nucleotide sequence is comprised by another polynucleotide, wherein the other polynucleotide includes:
(a) a second nucleotide sequence, wherein the second nucleotide sequence encodes a polypeptide having chalcone synthase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2 or 4 having at least 80% sequence identity, or (b) the complement of the second nucleotide sequence of (a).
- 15. An isolated polypeptide having chalcone synthase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2 or 4 have at least 80% identity.
- 16. The polypeptide of claim 15, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2 or 4 have at least 85% identity.
- 17. The polypeptide of claim 15, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2 or 4 have at least 90% identity.
- 18. The polypeptide of claim 15, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2 or 4 have at least 95% identity.
- 19. The polypeptide of claim 15, wherein the amino acid sequence of the polypeptide comprises the amino acid sequence of SEQ ID NO:2 or 4.
- 20. A method for isolating a polypeptide encoded by the polynucleotide of claim 1 comprising isolating the polypeptide from a cell or culture medium of the cell, wherein the cell comprises a recombinant DNA construct comprising the polynucleotide of claim 1 operably linked to at least one regulatory sequence.
Parent Case Info
[0001] This application claims the benefit of U.S. Provisional Application No. 60/404,171, filed Aug. 16, 2002, the entire content of which is herein incorporated by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60404171 |
Aug 2002 |
US |