Characterization of Proteins and Their Aggregates at High Concentration

Information

  • Research Project
  • 7107739
  • ApplicationId
    7107739
  • Core Project Number
    R43GM077722
  • Full Project Number
    1R43GM077722-01
  • Serial Number
    77722
  • FOA Number
  • Sub Project Id
  • Project Start Date
    8/1/2006 - 18 years ago
  • Project End Date
    1/30/2008 - 17 years ago
  • Program Officer Name
    SWAIN, AMY L
  • Budget Start Date
    8/1/2006 - 18 years ago
  • Budget End Date
    1/30/2008 - 17 years ago
  • Fiscal Year
    2006
  • Support Year
    1
  • Suffix
  • Award Notice Date
    7/28/2006 - 18 years ago

Characterization of Proteins and Their Aggregates at High Concentration

[unreadable] DESCRIPTION (provided by applicant): Scattering Solutions, LLC proposes to develop a method to characterize proteins and protein aggregation in highly concentrated suspensions by the combination of 3 new techniques: (1) cross correlation of light scattered within a single speckle to suppress the detrimental effect of multiple scattering; (2) optical homodyne amplification to suppress flare and errors arising from properties of the sample or its containment vessel; and (3) a new signal processing scheme to access a larger simultaneous dynamic range than formerly possible, with internally generated estimates of accuracy. Phase I research tasks build on previous research and development to confirm the feasibility of Scattering Solutions' light scattering technology for the characterization of highly concentrated proteins, and prepare for more advanced research and development which seeks to extend the range of measurements to include smaller sizes, dilute concentrations, small sample volumes, and shape characterization. This research specifically addresses stability issues which present efficacy and safety concerns to high concentration final dosage formulations of therapeutic agents. In addition, an investigation is proposed to examine the effect of protein concentration on fibril formation, a common structural feature in several degenerative conditions such as Parkinson's and Alzheimer's diseases. This research addresses the aims of NIH in its objective to lay the foundation for advances in disease diagnosis, treatment, and prevention since it addresses an issue which is vital to patient care (drug administration), pharmaceutical development, and fundamental biochemistry and medical research. Phase II will yield the design and fabrication of an inexpensive yet versatile instrument with highly desirable capabilities not currently available. It will also include extensive studies with commercial therapeutic proteins provided by multiple collaborators, and studies concerning disease-correlated protein aggregation phenomena. [unreadable] [unreadable] [unreadable]

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R43
  • Administering IC
    GM
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    156482
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    859
  • Ed Inst. Type
  • Funding ICs
    NIGMS:156482\
  • Funding Mechanism
  • Study Section
    ZRG1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    SCATTERING SOLUTIONS, LLC
  • Organization Department
  • Organization DUNS
    189584423
  • Organization City
    Irvine
  • Organization State
    CA
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    92612
  • Organization District
    UNITED STATES