Claims
- 1. A dioxetane of the formula II, III or IV: ##STR41## wherein Y.sup.1 and Y.sup.2 are independently H, a hydroxyl group, a halogen, an unsubstituted lower alkyl group, a hydroxy lower alkyl group, a halo lower alkyl group, a phenyl group, a halo phenyl group, an alkoxyphenoxy group, an alkoxy phenyl group, a hydroxyalkoxy group, a cyano group, an amide group, an alkoxy group or a carboxyl group,
- R is C1-12 alkyl, aryl or aralkyl,
- X is an enzyme-labile group selected from the group consisting of a phosphate, galactoside, acetate, 1-phospho-2,3-diacylglyceride, 1-thio-D-glucoside, adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, adenosine, .alpha.-D-glucoside, .beta.-D-glucoside, .beta.-D-glucuronide, .alpha.-D-mannoside, .beta.-D-mannoside, .beta.-D-fructofuranoside, .beta.-glucosiduronate, P-toluenesulfonyl-L-arginine ester, and P-toluenesulfonyl-L-arginine amide, and
- Z is an electron-active group selected from the group consisting of electron withdrawing groups and electron donating groups;
- with the proviso that OX is substituted on the naphthyl ring such that the number of ring carbons between the point of substitution of the dioxetane on the naphthyl ring, including the carbon at that point of substitution, and the point of substitution of OX, including the carbon at that point of substitution, is an odd number.
- 2. The dioxetane of claim 1 wherein X is a phosphate group.
Parent Case Info
This is a Continuation of application Ser. No. 08/588,810 filed on Jan. 19, 1996, now U.S. Pat. No. 5,851,771, which is a Continuation application of Ser. No. 08/057,903, filed on May 7, 1993, allowed, now U.S. Pat. No. 5,538,847 issued Jul. 23, 1996, which is a CIP of U.S. Ser. No. 07/806,928 filed Dec. 12, 1991, now U.S. Pat. No. 5,330,900, which is a divisional application of Ser. No. 07/574,786, filed Aug. 30, 1990, now U.S. Pat. No. 5,112,960, which is a CIP of Ser. No. 07/559,152, filed Jul. 25, 1990 now abandoned, which is a divisional application of Ser. No. 07/367,772, filed Jul. 17, 1989 now abandoned and a Division of Ser. No. 07/140,197, filed Dec. 31, 1987 now abandoned.
Foreign Referenced Citations (1)
Number |
Date |
Country |
8906226 |
Jul 1989 |
WOX |
Non-Patent Literature Citations (1)
Entry |
Edwards et al., J. Org. Chem., 55(25):6225-29, 1990. |
Divisions (2)
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Number |
Date |
Country |
Parent |
574786 |
Aug 1990 |
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Parent |
367772 |
Jul 1989 |
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Continuations (2)
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Number |
Date |
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Parent |
588810 |
Jan 1996 |
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Parent |
057903 |
May 1993 |
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Continuation in Parts (2)
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Number |
Date |
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Parent |
806928 |
Dec 1991 |
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Parent |
559152 |
Jul 1990 |
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