TREA

CHIMERIC RSV, IMMUNOGENIC COMPOSITIONS, AND METHODS OF USE

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Information

  • Patent Application
  • 20230293660
  • Publication Number
    20230293660
  • Date Filed
    January 14, 2022
    2 years ago
  • Date Published
    September 21, 2023
    a year ago
Information
Abstract
This disclosure relates to chimeric respiratory syncytial virus (RSV), live attenuated vaccine and immunogenic compositions, and methods of use. In certain embodiments, the chimeric respiratory syncytial virus has a mutated gene pattern encoding an RSV F protein having M at position 79, R at position 191, K at position 357, and/or Y at position 371.
Description
Claims
  • 1-20. (canceled)
  • 21. A respiratory syncytial virus (RSV) F protein comprising K at position 357 and Y at position 371, each relative to amino acid sequence as set forth in SEQ ID NO: 4, provided that the RSV F protein has less than 98% sequence identity to SEQ ID NO: 4; and wherein the RSV F protein has more than 90% sequence identity to SEQ ID NO: 1.
  • 22. The RSV F protein of claim 21, further comprising M at position 79, R at position 191, and/or V at position 557.
  • 23. The RSV F protein of claim 21, wherein the RSV F protein has more than 95% sequence identity to SEQ ID NO: 1.
  • 24. The RSV F protein of claim 21, wherein the RSV F protein comprises SEQ ID NO: 1.
  • 25. The RSV F protein of claim 21, wherein the RSV F protein is derived from an RSV strain of subgroup B.
  • 26. The RSV F protein of claim 21, wherein the RSV protein is derived from the Buenos Aires clade.
  • 27. The RSV F protein of claim 21, wherein the F protein is mutated such that position 557 is not V.
  • 28. The RSV F protein of claim 27, wherein the F protein is mutated such that I is in position 557.
  • 29. A respiratory syncytial virus (RSV) F protein comprising K at position 357 and Y at position 371, each relative to amino acid sequence as set forth in SEQ ID NO: 4, provided that the RSV F protein has less than 98% sequence identity to SEQ ID NO: 4; and wherein the RSV F protein has more than 90% sequence identity to SEQ ID NO: 13.
  • 30. The RSV F protein of claim 29, further comprising M at position 79, R at position 191, and V at position 557.
  • 31. The RSV F protein of claim 29, wherein the RSV F protein has more than 95% sequence identity to SEQ ID NO: 13.
  • 32. The RSV F protein of claim 29, wherein the RSV F protein comprises SEQ ID NO: 13.
  • 33. The RSV F protein of claim 29, wherein the RSV F protein is derived from an RSV strain of subgroup B.
  • 34. The RSV F protein of claim 29, wherein the RSV protein is derived from the Buenos Aires clade.
  • 35. The RSV F protein of claim 29, wherein the F protein is mutated such that position 557 is not V.
  • 36. The RSV F protein of claim 29, wherein the F protein is mutated such that I is in position 557.
Provisional Applications (2)
Number Date Country
62334547 May 2016 US
62247962 Oct 2015 US
Continuations (1)
Number Date Country
Parent 15772275 Apr 2018 US
Child 17575829 US