Chinese traditional medicine composition for treatment of avian influenza, method for preparation, and application thereof

Abstract

A Chinese traditional medicine composition for prevention and treatment of avian influenza is provided. Its activated ingredients are consisted of: herba houttuyniae 21.4 wt. %, Caulis lonicerae japonicae 17.9 wt. %, radix isatidis 14.3 wt. %, male fern rhizome 10.7 wt. %, radix et rhizoma sophorae tonkinensis 7.1 wt. %, radix angelicae dahuricae 7.1 wt. %, rhizoma paridis 7.l wt. %, herba artemisiae annuae 7.2 wt. %, and rhizoma iridis tectoris 7.2 wt. %. This Chinese traditional medicine composition could be made into any regular medicinal types, for example, oral solutions, tablets, capsules, pellets and injection solution, etc. Modified preparation methods for this Chinese traditional medicine composition, as well as the use of the same in preparation of medicines and health foods for prevention or treatment of avian influenza were also provided in present invention. This Chinese traditional medicine composition could effectively prevent and treat avian influenza. It was a safer, more easily-obtained, and inexpensive anti-avian influenza drug.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows an optical microscopic photograph of the cells of control group in Experiment Example 3, while the culture of cells was stopped. FIG. 1A shows a positive control group without medicine. FIG. 1B shows a control group applied with 0.284 ug/ml of Tamiflu, and a large amount of cells are infected. FIG. 1C shows a control group applied with 2.84 ug/ml of Tamiflu, and most of cells are infected. FIG. 1D shows a control group applied with 28.4 ug/ml of Tamiflu, wherein viruses was killed, but there are many unrelated cells (killed).

FIG. 2 shows optical microscopic photograph of the cells of control group in Experiment Example 3, while the culture of cells was stopped. FIG. 2A shows a positive control group without medicine. FIG. 2B shows the photograph when applied with 12.5 ug/ml of the Chinese traditional medicine composition of the present invention, and a large amount of cells are infected. FIG. 2C shows photograph when applied with 25 ug/ml of the Chinese traditional medicine composition of the present invention, and a large amount of cells are infected. FIG. 2D shows the photograph when applied with 50 ug/ml of the Chinese traditional medicine composition of the present invention, wherein the viruses are killed, and only a few cells are infected. FIG. 2E shows the photograph when applied with 100 ug/ml of the Chinese traditional medicine composition of the present invention, wherein all viruses are killed, and there are a few unrelated cells (killed). FIG. 2F shows the photograph when applied with 200 ug/ml of the Chinese traditional medicine composition of the present invention, wherein all viruses are killed, but some cells are killed.

Claims

1. A Chinese traditional medicine composition for treatment and prevention of Avian Influenza comprising active ingredients and/or medical acceptable vectors, wherein the said active ingredients include the following raw materials: herba houttuyniae 4.7 wt. %-70.0 wt. %, caulis lonicerae japonicae 4.7 wt. %-46.7 wt. %, radix isatidis 2.3 wt. %-42.0 wt. %, radix et rhizoma sophorae tonkinensis 1.2 wt. %-35.0 wt. % and other herbs for removing fever and detoxification.

2. The Chinese traditional medicine composition according to claim 1, wherein the said herbs for removing fever and detoxification are Male Rern Rhizome, Radix angelicae dahuricae, Herba Andrographic, Dandelion, Natural Indigo, Sargentgloryvine Stem and Chinese Licorice.

3. The Chinese traditional medicine composition according to claim 1, wherein the said active ingredients are consisted of herba houttuyniae 4.7 wt. %-70.0 wt. %, Caulis lonicerae japonicae 4.7 wt. %-46.7 wt. %, radix isatidis 2.3 wt. %-42.0 wt. %, and radix et rhizoma sophorae tonkinensis 1.2 wt. %-35.0 wt. %.

4. A Chinese traditional medicine composition for treatment and prevention of Avian Influenza comprising active ingredients and/or medical acceptable vectors, wherein the said active ingredients include the following raw materials: herba houttuyniae 4.7 wt. %-58.4 wt. %, caulis lonicerae japonicae 4.7 wt. %-46.7 wt. %, radix isatidis 2.3 wt. %-42.0 wt. %, radix et rhizoma sophorae tonkinensis 1.2 wt. %-35.0 wt. %, male fern rhizome 1.9 wt. %-37.3 wt. %, radix angelicae dahuricae 1.2 wt. %-35.0 wt. % and other herbs for removing fever and detoxification.

5. The Chinese traditional medicine composition according to claim 4, wherein the said herbs for removing fever and detoxification are rhizoma paridis, herba artemisiae annuae, Java brucea fruit, herba andrographis, dandelion, mint, platycodon grandiflorus, and Chinese licorice.

6. The Chinese traditional medicine composition according to claim 4, wherein the said active ingredients are consisted of the following raw materials: herba houttuyniae 4.7 wt. %-58.4 wt. %, Caulis lonicerae japonicae 4.7 wt. %-46.7 wt. %, radix isatidis 2.3 wt. %-42.0 wt. %, and radix et rhizoma sophorae tonkinensis 1.2 wt. %-35.0 wt. %, male fern rhizome 1.9 wt. %-37.3 wt. %, radix angelicae dahuricae 1.2 wt. %-35.0 wt. %.

7. A Chinese traditional medicine composition for treatment and prevention of Avian Influenza comprising active ingredients and/or medical acceptable vectors, wherein the said active ingredients are consisted of the following raw materials: herba houttuyniae 9.3 wt. %-46.7 wt. %, Caulis lonicerae japonicae 9.3 wt. %-37.3 wt. %, radix isatidis 4.7 wt. %-35.0 wt. %, male fern rhizome 2.3 wt. %-28.0 wt. %, radix et rhizoma sophorae tonkinensis 2.3 wt. %-23.3 wt. %, radix angelicae dahuricae 2.3 wt. %-23.3 wt. %, rhizoma paridis 2.3 wt. %-23.3 wt. %, herba artemisiae annuae 2.3 wt. %-23.3 wt. %, rhizoma iridis tectoris 2.3 wt. %-23.3 wt. %.

8. The Chinese traditional medicine composition according to claim 7, wherein the said active ingredients are consisted of the following raw materials: herba houttuyniae 14.0 wt. %-35.0 wt. %, Caulis lonicerae japonicae 11.7 wt. %-23.3 wt. %, radix isatidis 9.3 wt. %-21.0 wt. %, male fern rhizome 7.0 wt. %-18.7 wt. %, radix et rhizoma sophorae tonkinensis 4.7 wt. %-11.7 wt. %, radix angelicae dahuricae 4.7 wt. %-11.7 wt. %, rhizoma paridis 4.7 wt. %-11.7 wt. %, herba artemisiae annuae 4.7 wt. %-11.7 wt. %, rhizoma iridis tectoris 4.7 wt. %-11.7 wt. %.

9. The Chinese traditional medicine composition according to claim 8, wherein the said active ingredients are consisted of the following raw materials: herba houttuyniae 21.4 wt. %, Caulis lonicerae japonicae 17.9 wt. %, radix isatidis 14.3 wt. %, male fern rhizome 10.7 wt. %, radix et rhizoma sophorae tonkinensis 7.1 wt. %, radix angelicae dahuricae 7.1 wt. %, rhizoma paridis 7.1 wt. %, herba artemisiae annuae 7.2 wt. %, rhizoma iridis tectoris 7.2 wt. %.

10. The Chinese traditional medicine composition according to claims 1-9, wherein the said Chinese traditional medicine composition is in any of the medicine types.

11. The Chinese traditional medicine composition according to claim 10, wherein the said Chinese traditional medicine composition is a pellet.

12. A preparation method for the Chinese traditional medicine composition according to any of claims 1-11, wherein the method includes the following steps: (1) all raw materials are weighed;(2) the herba houttuyniae is boiled in water for 10˜40 minutes, and filtered, then the filtrate is concentrated to obtain a clear cream with a density of 1.05, and cooled;(3) the clear cream from step 2 is added with ethanol until the amount of ethanol is 50˜80%, allowed to sit for 24 hours and filtered, then ethanol is recovered from the filtrate and concentrated to obtain a clear cream with a density of 1.25;(4) other raw materials are boiled in water twice for 2 hours each time and filtered, then the filtrate is concentrated to a density of 1.20, filtered, and concentrated again to obtain a clear cream with a density of 1.10-1.25;(5) the clear cream from step 3 and step 4 are mixed, concentrated continuously to obtain a thick cream with a density of 1.26 or above, and added with accessory agents.

13. The use of the Chinese traditional medicine composition according to claims 1-11 in preparation of medicines for prevention or treatment of avian influenza.

14. The use of the Chinese traditional medicine composition according to claims 1-11 in preparation of health foods for prevention or treatment of avian influenza.

15. The use according to claim 13 or 14, wherein the said avian influenza is highly pathogenic avian influenza.

16. The use according to claim 15, wherein the said highly pathogenic avian influenza is caused by H5N1.

17. The use according to claim 15, wherein the said avian influenza is human-avian influenza.

18. The use according to claim 13, wherein the said avian influenza is animal-avian influenza.