Claims
- 1. A method of eliciting in a vertebrate a protective immune response against a bacterium of the genus Chlamydia, the method comprising administering to the vertebrate a composition comprising a carrier group coupled to an oligosaccharide obtained from a chlamydial glycolipid, the composition being administered in an amount sufficient to elicit a protective immune response against the member.
- 2. The method of claim 1, wherein the chlamydial glycolipid is glycolipid exoantigen.
- 3. The method of claim 1, wherein the carrier group is coupled to the oligosaccharide by a linker.
- 4. The method of claim 3, wherein the linker is 2-(4-aminophenyl)ethylamine.
- 5. The method of claim 1, wherein the carrier group is coupled to a mixture of oligosaccharides obtained from the glycolipid.
- 6. The method of claim 5, wherein the mixture of oligosaccharides comprises oligosaccharides having a molecular weight of from 800 to 3000 daltons.
- 7. A composition comprising a carrier group coupled to an oligosaccharide obtained from a chlamydial glycolipid.
- 8. The composition of claim 7, wherein the glycolipid is GLXA.
- 9. The composition of claim 7, wherein the carrier group is coupled to the oligosaccharide by a linker.
- 10. The composition of claim 9, wherein the linker is 2-(4-aminophenyl)ethylamine.
- 11. A method of purifying a chlamydial glycolipid, the method comprising providing an aqueous composition that has been in contact with cells infected with a bacterium of the genus Chlamydia, the aqueous composition comprising a chlamydial glycolipid; centrifuging the composition for at least 2 hours at 100,000 g or more to form a pellet comprising the chlamydial glycolipid; and collecting the pellet, thereby purifying the chlamydial glycolipid.
- 12. The method of claim 11, further comprising centrifuging an aqueous mixture at 8000 g or less to produce the aqueous composition.
- 13. The method of claim 11, further comprising resuspending the pellet in a reaction mixture and digesting the reaction mixture with DNAse, RNAse, and proteinase K to form a digested mixture.
- 14. The method of claim 13, further comprising subjecting the digested mixture to affinity chromatography using a monoclonal antibody against chlamydial glycolipid exoantigen.
- 15. A purified chlamydial glycolipid exoantigen, wherein the purified chlamydial glycolipid exoantigen is free of other components as determined by sodium dodecylsulfate gel electrophoreses and silver staining.
- 16. A method of eliciting in a vertebrate a protective immune response against a bacterium of the genus Chlamydia, the method comprising administering to the vertebrate a composition comprising a carrier group coupled to an oligosaccharide corresponding to a chlamydial glycolipid, the composition being administered in an amount sufficient to elicit a protective immune response against the member.
- 17. A composition comprising a carrier group coupled to an oligosaccharide corresponding to a chlamydial glycolipid.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority from U.S. Provisional Patent application Ser. No. 60/195,004, filed Apr. 6, 2000.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60195004 |
Apr 2000 |
US |