Claims
- 1. A method for the detection and determination of endoproteases comprising:
- a) incubating a solution suspected of containing an endoprotease with a solution containing a chromogenic peptide substrate for said endoprotease, said chromogenic peptide substrate comprising a compound of the formula I ##STR5## in which X denotes a hydrogen atom, a group which irreversibly masks the terminal amino group, or a conventional protecting group selected from the group consisting of carbobenzoxy, t-butoxycarbonyl and 9-fluorenyloxycarbonyl,
- A and B may be identical or different and denote an alpha-, beta- or gamma-amino acid which comprises 2 to 15 carbon atoms and up to 4 nitrogen atoms, 2 sulfur atoms and 6 oxygen atoms, and B may denote a dipeptide formed from these amino acids,
- C denotes arginine, lysine, tyrosine, phenylalanine or tryptophane, and their homologs,
- R.sub.1 and R.sub.2 may be identical or different and denote a hydrogen atom or an alkyl radical having up to 4 carbon atoms,
- R.sub.3 to R.sub.8 may be identical or different and denote hydrogen, an alkyl radical, an alkoxy radical or a halogen radical,
- Y denotes oxygen, and
- An.sup.- denotes an anion,
- and its water-soluble salts, and
- b) determining the amount of chromophore released by said endoprotease from said chromogenic peptide substrate.
- 2. The method of claim 1, in which X is a hydrogen atom and the amino acid A is present in the D-form, if A is a chiral amino acid.
- 3. The method of claim 1, wherein
- R.sub.1 and R.sub.2 are identical or different and are selected from the group consisting of methyl, ethyl, propyl and hydrogen, and
- R.sub.7 is selected from a group consisting of hydrogen, methyl, ethyl, propyl, methoxy and ethoxy.
Priority Claims (1)
Number |
Date |
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Kind |
3629175 |
Aug 1986 |
DEX |
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Parent Case Info
This is a division of application Ser. No. 07/089,425, filed Aug. 26, 1987 and now U.S. Pat. No. 5,097,014.
US Referenced Citations (10)
Divisions (1)
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Number |
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89425 |
Aug 1987 |
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