Patent Application
20240382082
The subject matter disclosed herein relates to a cleaning indicator that is used to determine if articles that are cleaned in a washing machine, such as an endoscope reprocessing system, are in fact clean to a necessary degree following operation of the washing machine.
It is sometimes necessary to determine the efficacy of a cleaning process. For example, before being subjected to sterilization, instruments used in a surgical procedure must be cleaned to remove soils such as blood, stool, or other body fluids that remain on them after use. Even new instruments must sometimes be cleaned to remove substances left on their surfaces during manufacture. As used herein, a “cleaning process” or “washing process” relates to washing of articles in a washing machine, which can be operated manually or automatically.
The effectiveness of a cleaning process can be verified by use of a cleaning indicator, which indicator can be one containing an active agent, such as a color changing chemical sensitive to a specific chemistry or physical condition such as temperature, and a carrier substrate (i.e., support system) for supporting the active agent. Effectiveness of a cleaning process is indicated by a change in the color of the active agent, which is then evaluated by an operator, for example by visual inspection, after the cleaning process is completed. Alternatively, the indicator can comprise a substrate having an image printed on it in an ink that is susceptible to being washed off the substrate by the cleaning process, and effectiveness of the cleaning process is evaluated by visual observation of the image to determine the completeness of its removal.
One aspect of the present disclosure relates to a cleaning indicator comprising at least one substrate including an image side bearing at least one query image and at least one test soil covering at least a portion of the at least one query image. In an alternative embodiment, a cleaning indicator can comprise at least one substrate including an image side bearing at least one query image that is formed from a test soil.
This aspect of the present disclosure includes an article comprising such a cleaning indicator, and further comprising a holder that encompasses the cleaning indicator and includes passages of various shapes and sizes disposed proximate to the image side.
Another aspect of the present disclosure relates to a method for monitoring cleaning of articles comprising:
Some embodiments of this aspect of the disclosure may further comprise measuring a starting reflectance or fluorescence of the blank image before step ii, and wherein the determining step includes determining that the exposed portion of the at least one query image provides a reflectance or fluorescence within 95% of the starting reflectance.
Another aspect of the present disclosure is embodied in a washing machine system comprising
A further aspect of the present disclosure lies in a method for monitoring cleaning of articles comprising:
The specification concludes with claims, which particularly point out and distinctly claim the subject matter described herein and so define the invention disclosed herein. The accompanying drawings, which are merely illustrative, and should not be considered as describing all embodiments or otherwise limiting the invention that is encompassed by the claims, are provided to assist in understanding the disclosed invention. In the drawings, like reference numerals identify the same elements.
The following detailed description should be read with reference to the drawings, in which like elements in different drawings are identically numbered. The drawings, which are not necessarily to scale, depict selected embodiments and are not intended to limit the scope of the invention. The detailed description illustrates by way of example, not by way of limitation, the principles of the invention. This description will clearly enable one skilled in the art to make and use the invention, and describes several embodiments, adaptations, variations, alternatives and uses of the invention, including what is presently believed to be the best mode of carrying out the invention.
As used herein, the terms “about” or “approximately” for any numerical values or ranges indicate a suitable dimensional tolerance that allows the part or collection of components to function for its intended purpose as described herein. More specifically, “about” or “approximately” may refer to the range of values ±10% of the recited value, e.g. “about 90%” may refer to the range of values from 81% to 99%.
Presently disclosed is a cleaning indicator that can be used to evaluate the efficacy of a cleaning process conducted in a washing machine. Such a cleaning process is one intended to remove various soils from articles, for example instruments or medical devices used in a surgical or other medical procedure, but unless otherwise stated, the articles to be washed are not so limited. In some embodiments, the washing machine works automatically; in relation to the present disclosure, particular automatic operations can be those with respect to ending the washing procedure (or “washing cycle”) and unlocking of the machine. An automatic operation can also be one of starting and further controlling a washing procedure after manually pushing a “start” button by an operator of the washing machine.
Typically, cleaning indicators must be inspected manually, i.e., by visual evaluation of the indicator by one conducting a washing process, with the attendant problems of interruption of the cleaning process to confirm that it has been effective, and the subjectivity of the evaluation of efficacy of the cleaning process that has been conducted. The cleaning indicators presently disclosed can be used in such a fashion, but they also improve the existing technology by enabling automatic detection of the effectiveness of the washing process, without inspection of the cleaning indicator by a person. Thus, an ineffective cleaning process can be indicated to an operator as ineffective such that the process may be repeated or modified to make it effective, or can be continued without interruption to a point where it has become effective as indicated by the cleaning indicator. Continuing a cleaning process can include modifying the conditions of the cleaning process.
Another improvement to the existing technology concerns incorporating the cleaning indicator into a system that includes instrumentation for scanning the cleaning indicator and, if the cleaning indicator is not one that is recognized by the instrumentation as being genuine, or the cleaning indicator being used is of the wrong type (e.g. having a substrate requiring different cleaning conditions from those programmed into the washing machine to clean those articles being washed) the operator can be so informed, automatic operation of the washing machine can be stopped or prevented, or the washing machine may be locked.
In general, a cleaning indicator can be one comprising a substrate bearing (or showing) at least one query image on an image side and at least one test soil deposited on the image side of the substrate and covering at least a portion of the query image.
Such a cleaning indicator can be one wherein the query image comprises a first image portion and a second image portion, the first image portion being covered by a first test soil and the second image portion being covered by a second test soil different from the first test soil.
Such a cleaning indicator can be one further comprising a plurality of substrates, each substrate bearing at least one query image on an image side and at least one test soil deposited on the image side of the substrate and covering at least a portion of the query image.
A “query image” is one that is inspected, either visually by one conducting a washing process, or by devices included in a washing machine system, to determine whether the cleaning process has been effective to remove soils from articles being cleaned by the cleaning process. A query image can be in the form of a barcode or a QR code, a simple geometric shape (such as a circle or regular polygon—e.g. a triangle or a square) or a random but pre-determined and consistently drawn shape. A query image can be “filled”, i.e. the area within the outline of the query image is printed, preferably with the same ink as used to print the outline, or can be presented only as an outline and having the substrate in the outlined area not covered by any ink. A query image can comprise a shape that includes a plurality of concave and convex portions. A query image can include a plurality of shapes that are not connected to one another.
A query image can be embossed on the substrate, leaving a depression in the substrate.
In some embodiments, the query image is completely covered by a soil intended to be removed by the washing process (a “test soil”) and an effective washing process is one that removes all of the soil from the query image so that the complete query image is seen upon visual inspection by the operator, or the complete query image is detected by a scanning device that is part of a washing system using the cleaning indicator.
Test soils can be either actual body fluids—e.g. blood or urine, tissues, excrements or the like, or “proxy” soils, as are known in the art, such as a Chemdye®, or a fat or carbohydrate substance. Some examples of test soils are listed in ISO 15883, including: nigrosin, wheat flour, hens egg, E. faecium, blood, bovine hemoglobin, P. aeruginosa biofilm, bovine serum albumin, porcine mucin, bovine fibrinogen, glycerol, dehydrated hog mucin, horse serum, unbleached plain flour, and an aqueous safranine solution.
Test soils can comprise a mixture of two or more compositions or substances. In some embodiments, the test soil is applied to the substrate to cover the desired portion of the query image and dried to bind it to the cleaning indicator.
In some embodiments, a depression of an embossed query image is filled with the test soil.
In some embodiments, a portion of the query image is left uncovered by any test soil, and such an uncovered portion can be interrogated as a “blank” portion of the image that serves as a reference for reflectance or fluorescence measurement of a clean surface bearing the ink used to produce the query image. In some embodiments, the substrate is printed with both of at least one query image and a separate blank image, using the same ink. In such embodiments, a query image is covered with one or more test soils and the blank image is left uncovered by any soil.
Any material durable in the washing process in which the cleaning indicator is to be used might be used as a substrate. Preferably, the substrate is one that provides a rigid, or at least only slightly flexible, surface suitable for handling without flaking of the ink used to print the images on the substrate and preferably without flaking of the test soil applied over the query image. Suitable substrates include laminated paper, a plastic—for example, polycarbonate or polystyrene or polymethylmethacrylate, a glass, a metal, or a suitably stiff rubber. The substrate can be a laminated paper, a plastic, a rubber, a metal, a ceramic, a glass or a composite of any two or more of these. In some embodiments, the substrate is a material similar to the material used to manufacture a part or the whole of an article being cleaned in the washing process in which the cleaning indicator is used. For instance, a substrate might be made from the same rubber used to produce the tubing portions of an endoscope. A borosilicate or pyrex glass might be used as a substrate for a cleaning indicator for a process used to wash laboratory glassware.
A cleaning indicator as disclosed can also include a magnetic strip or RFID chip that can hold information relating to the indicator and integrating the handling of the cleaning indicator or its associated articles into a hospital or laboratory supply chain management system. Alternatively, such information can be encoded in a barcode or QR code as part of a blank image or a query image.
In some embodiments, the cleaning indicator includes a plurality of substrates, each underlying a query image, or a portion of one query image, that is covered with the test soil. For instance, a substrate comprising a section of glass, a section of rubber and a section of plastic can be overlaid by a single query image and the query image then covered by a test soil. (Transparent or translucent substrates might be underlaid by a query image so that the image shows through the substrate on an image side bearing a test soil.) In some of these embodiments, each substrate material can be covered, in whole or in part, by a different query image. In some embodiments, the query image can include blank portions that allow interrogation of each substrate uncovered by either soil or ink that can serve as a reference surface for measurements of fluorescence or reflectance of each substrate.
Such embodiments as above can be made by adhering each different substrate to an underlying sheet of a suitable material, such as a laminated paper, a plastic, a rubber, a metal, a ceramic, a glass or a composite of any two or more of these
Additionally or alternatively, any query image can be covered by a plurality of test soils of different compositions.
In some embodiments, the test soil can be applied to the cleaning indicator in a shape, or in a shape and color, that can be detected prior to the start of the cleaning cycle, and if that shape (and/or color) does not match an image stored by a washing machine utilizing the cleaning indicator, then the machine is not started. In some embodiments, the test soil can be formed into a shape that is used as the query image. In such embodiments, an area of the substrate not covered by the test soil formed into the query image can be used as a blank image or as a measurement standard for determining that the test soil has been completely removed from the substrate. In such an embodiment, the criteria for deeming that the washing cycle has been successfully completed can be that the reflectance of a light beam from the area covered by the test soil or transmittance of a light beam through the area of the test soil (if the substrate is transparent or translucent) is the same as the reflectance or transmittance of a reference light beam from the blank area of the substrate.
The ink that is used to print the query image can be any ink known in the art to bind to the selected substrate and to remain bound to the substrate under the conditions of the washing process. For example, Platinum Carbon Black ink is a highly water resistant pigment-based ink; Diamine Registrar's Blue-black ink is a waterproof iron gall ink. In some embodiments, the ink used to print the query image can comprise a fluorophore, preferably one that remains bound to the cleaning indicator under the conditions of the washing process (for example, perylene is a water insoluble flurophore). In some such embodiments, the cleaning indicator also includes a blank image printed on the substrate(s) using the same ink as used to print the query image.
In some embodiments, the substrate of the cleaning indicator can be transparent to optical or ultraviolet wavelengths, and the query image can be detected as a shadow in a transmittance image. In some such embodiments, an unprinted part of the substrate can serve as a blank image or blank portion of a query image.
Referring to
A cleaning indicator as disclosed herein can be used together with an article that serves to hold the cleaning indicator in a particular position, for example, such as a position in which the cleaning indicator may be scanned by a device that images the query image, or in some embodiments that images both the query image and a blank image. Such an article can be a holder that encompasses the cleaning indicator and includes at least one opening, such as passageways of various shape and size, through which the test soil surface of the cleaning indicator may be exposed to the cleaning environment inside a cleaning system or washing machine system, such that during a washing cycle, the cleaning indicator and particularly the test soil may be exposed to cleaning fluids. A holder for a cleaning indicator can be a simple frame holding two or more of the edges of the cleaning indicator, or might be configured as a “claw” that embraces the edges of a cleaning indicator.
The holding article will have at least one opening, such as the openings described in the preceding paragraph, or at least one alternative opening, through which light can pass from an image on the cleaning indicator to the scanning or imaging device (e.g. a digital camera or a bar-code or QR code reader). In some embodiments the holding article can also have at least one opening through which light from the scanning or imaging device can illuminate an image on the cleaning indicator.
One embodiment of such a holder is illustrated in
One or more cleaning indicators (and holders) as described above can be used in a washing processes implemented in a washing machine system. Such as system can be one that includes an enclosure holding within it apparatus suitable for holding articles to be washed, fluid handling systems for washing and rinsing articles to be washed, in some instances a system for venting aerosols and the like and for drying the articles after they have been washed, a holder that positions a cleaning indicator as disclosed herein to be imaged by an imaging apparatus for obtaining images of the cleaning indicator at one or more times in the washing process. The holder should also position the cleaning indicator in a manner that exposes the test soil surface and any blank image of the cleaning indicator to the cleaning process of the washing machine, and preferably exposes them to the cleaning environment of the washing machine. The holder might be accessible from the outside of the washing machine through an opening different from the one used to load articles into the machine.
The imaging apparatus can include a digital camera, which can be one configured to image a fluorescent emission or a reflected or transmitted light beam. In instances where a query and/or a blank image comprises a bar code or a QR code, the imaging apparatus can be a bar code or QR code scanner. The imaging apparatus can include hardware and software for illuminating an object to be imaged, for controlling illumination of an object to be imaged and for controlling exposure of an imaging sensor to the light coming from the object being imaged. Control of illumination and exposure can in some instances be by computational resources external to the imaging apparatus, or control of these can be divided between the imaging apparatus and external computational resources. (For example an external processor might control illumination and provide data to the imaging apparatus to set the appropriate exposure.) Fiber optic elements might convey light from a light acquiring element, such as a lens, to an imaging sensor. Such an arrangement might be used to dispose certain light gathering elements within the enclosure of washing machine and other imaging elements outside the enclosure of the washing machine. Also, multiple cleaning indicators might be disposed in different locations in the washing machine enclosure and convey images to a single imaging apparatus via such fiber optic elements.
The imaging apparatus can further comprise hardware and software elements, including but not limited to memory, a non-transitory storage medium (e.g., a solid state or disk hard drive) and processors (e.g., microprocessors) configured for running appropriate image acquisition and analysis software, for analyzing image data gathered by the imaging apparatus to determine whether a shape in the image corresponds to a shape stored in a memory module of the imaging apparatus, and for signaling to an electronic control in the washing machine whether a corresponding image has been detected or alternatively, that a different image has been detected. Alternatively, such image analysis hardware/software might be provided wholly or partly by computational resources external to the imaging apparatus and even external to the washing machine.
An image “corresponds to” or “matches” another image (e.g. a query image may correspond to a stored image) if the outlines of the shapes are the same or substantially the same when overlaid one upon the other. The criterion for correspondence can also include that a predetermined proportion of the length of the outline of the first image is found in the second image. This definition can be used with either line drawing images or filled shape images. In the instance of a filled shape image, a predetermined portion of the area of the filled image can also be used to define correspondence of two images. Preferably the proportion of the outline or filled shape detected matches at least 90%, more preferably 95%, or 99% of the complete outline or filled shape.
The criteria for correspondence or matching can additionally or alternatively include a threshold for a percentage of an initial reflectance, transmittance or fluorescence signal from a blank image or blank portion of a query image. Preferably, the percentage is set at least at 90%, more preferably 95% or 99% of the initial reflectance, transmittance or fluorescence, integrated over the entire image.
The washing machine system might further include computational resources that interface with material supply chain management systems in an enterprise. Such computational resources might collate an identification of the articles washed in a machine cycle with the data regarding the correspondence (or not) of the washed cleaning indicator to a “clean” indication after the cycle and perhaps also provide the current location of the articles as resident in the washing machine, as well as perhaps other data.
The washing machine system might also include electronic controls of its door locks or start switch that are configured to maintain the door locks in the “locked” position, and/or the start switch in the “off” position, in the event that the electronic control is signaled by the imaging apparatus that a query image corresponding to a stored image has not been received or if the control is signaled that a query image different from a stored image in its shape has been detected, or the control might be configured to maintain the door locks in the locked position until a signal is received that a “clean” indication has been detected.
One or more holders 309, which are configured to hold a cleaning indicator as otherwise described herein and to expose the cleaning indicator, at least the portion of it including the test soil-covered query image, and possibly the blank image or blank portion of the query image, to the washing environment, including the cleaning substances delivered by the end parts of the fluid handling system. The holders 309 are configured also to position the cleaning indicator, again including the test soil-covered query image, and possibly the blank image or blank portion of the query image, as might be applicable, to be imaged by an imaging system 311. In the illustrated embodiment the imaging system includes a digital camera 311a, that images the cleaning indicator through a lens 311b and, in some embodiments, a fiber optic element 311c. The imaging system illustrated also includes an illumination source 311d, that conveys light to illuminate the cleaning indicator, e.g., via a fiber optic element 311c.
Holder or primary holder 461 may be used in conjunction with a secondary holder 462, shown in
Flow restrictors incorporated directly in fluidic connectors 472 as well as flow restrictor 486 may function to restrict flow according to various mechanisms. For example, these flow restrictors may change a speed of the fluid by reducing a diameter of the flow path or may change a conductance of the fluid, e.g., by providing a plurality of empty volumes along the flow path such that the fluid must fill each volume before it can flow to the next volume, and ultimately to the cleaning indicator. Preferably, these flow restrictors, used singularly or in combinations, cause the flow properties (e.g., volume flow rate, mass flow rate, outlet pressure) of a cleaning liquid through primary holder 461 to be similar to the flow properties at a distal end of an endoscope lumen being cleaned in a washing machine system, such as system 301.
The article of claim 25, wherein the flow restrictor is configured is configured to change a conductance of the fluid.
The article of claim 25, wherein the flow restrictor comprises a plurality of empty volumes within the fluid path.
The imaging system conveys data to computational resources 313, which perform image-analysis to determine whether the “clean” criteria established for the washing machine system have been met. The results of the image analysis might also be used to determine whether a cleaning indicator appropriate for the washing procedure to be performed is present in the holder as described above. In the illustrated embodiment, the computational resources signal to a door locking mechanism 315 the result of image analysis in a manner that, if the “clean” criterion is not met, then the locking mechanism remains locked and the mechanism is unlocked if the “clean” criterion is met. In the illustrated embodiment, the computational resources also signal to a power switch 317 the result of analysis of a blank image of the cleaning indicator to remain “off” if the blank image does not correspond to an image stored in memory. However, if the blank image corresponds to the image stored in memory, the power switch may be activated to turn on washing machine system 301.
Computational resources 313 include a digital computer, e.g. a laptop or mobile device, or a networked computer, to control the operation of the washing machine system and its various components. Computational resources can employ one or more processors (e.g., microprocessors). Computational resources can also employ a non-transitory storage medium, such as random access memory (RAM), a hard-disk drive, or flash memory, which can store data, such as a manifest of articles being cleaned, control images, initial and time series fluorescence or transmittance data and the like, images constructed from data acquired by the imaging system, washing protocols including detergent nature and amount values, water temperature values and time values. Computational resources can further include software and/or logic for controlling a digital imaging system, which can include a fluorescence-based imaging system. Such software/logic can include instructions for controlling illumination, controlling a camera to acquire and produce an image, and to receive one or more acquired images from a camera. Computational resources can further include software and/or logic by which the microprocessor can numerically analyze image data. Computational resources can further include software and/or logic by which the microprocessor can compare a captured query image to a stored control image. Elements of camera control and image acquisition software/logic can be distributed between camera and digital computer devices. Computational resources can further include outputs to a display, for instance to show in some desired format a washing protocol that is run, and/or the results of comparison of the query and control images and/or fluorescence or transmittance data. Computational resources can also include software/logic for reporting results of a washing protocol (i.e. “successfully cleaned” or “not successfully cleaned”, or the like), perhaps together with associated inventory information relating to articles being washed, to an inventory management system or the like implemented by a washing facility. Computational resources can also include software/logic for controlling various parts of the washing machine system, for example a locking system that opens a lock upon determination that a washing cycle has sufficiently cleaned articles being washed, or for example that controls a switch starting the wash cycle in a manner responsive to analysis of a control image.
Further disclosed herein are methods for monitoring cleaning of articles. Such methods are preferably performed in automated washing machines.
A disclosed method can be one comprising:
A cleaning indicator as disclosed herein can be used in a manual method, in which the cleaning indicator is scanned by a hand-held reader, thereby enabling objective inspection of the indicator.
A method for monitoring cleaning of articles as disclosed herein can be one in which the cleaning indicator further comprises a blank image printed upon the image side using the same ink composition used to print the query image. Alternatively, such a method can be one in which the cleaning indicator includes a blank portion of the query image that is not covered by a test soil.
A disclosed method can be one that includes a step of measuring a starting reflectance, transmittance or fluorescence (luminosity) of a blank image, or of a blank portion of a query image, before the washing cycle of the washing machine system is started. Such a measurement of a starting reflectance, transmittance or starting fluorescence provides a reference measurement of a clean image that has no soil or residue from the cleaning process covering it. Thus, such a measurement provides a criterion for “clean” against which the progress of the cleaning process can be evaluated to determine automatically, i.e. without intervention of the operator of the washing machine if the washing process should be ended. Then, the washing process can be automatically continued until the reference criterion is met, at which time the washing process can be stopped. Alternatively, if the “clean criterion” is not met after the passage of a certain amount of time, an operator can intervene to change the washing conditions (perhaps including adding another cleaning indicator appropriate for the changed conditions) or the batch of washed articles can be flagged as inadequately cleaned and disposed of accordingly.
Such an automatically controlled washing process can be one including stopping the wash cycle only after the entire shape or substantially the entire shape of the query image is detected. Substantially the entire shape of the query image can be defined to include at least 90%, preferably at least 95%, or at least 99% of the complete outline of the query shape or of the complete filled area of the query shape (as might be applicable). In embodiments in which it is applicable, the automatically controlled washing process can be stopped when the query shape is detected at a reflectance or fluorescence of at least 90%, preferably at least 95% or at least 99%, of the starting reflectance or fluorescence integrated over the entire blank image.
In such a method, the query image can be scanned at the end of the washing cycle. In such an embodiment, the washing cycle can be re-started or continued if a deficient cleaning is detected, for instance by detecting a level or fluorescence or reflectance of the query image that is below the starting fluorescence or reflectance measured from the blank image (or blank portion of the query image), by some predetermined amount, or by failure to detect a complete shape of a query image.
An automatically controlled washing process can also be one in which the query image is scanned periodically or continuously during the washing cycle. In such an embodiment, the washing cycle can then be stopped when a reflectance or fluorescence value of the query image reaches a criterion value, or when a complete shape of a query image is detected, or a predetermined criterion of a combination of these variables is reached.
In some embodiments a blank image or the blank portion of a query image is scanned at the same time or in alternation with scanning of a corresponding query image (i.e. one printed with the same ink and on the same substrate as the blank). Such embodiments exhibit an advantage of being self-correcting, for example for fluctuations in reflectance or fluorescence measurements resulting from fluctuation in the intensity of the scanning or excitation beam, or due to scattering of the scanning or excitation beam or the signal beam by falling water drops or streams or fog occurring within the washing machine. In such embodiments, a time average of reflectance or fluorescence measurements in a manner known in the art are used to establish criterion for controlling the washing machine.
Embodiments of the presently disclosed method can include scanning of a blank image and preventing start of the wash cycle if the blank image detected by the scan does not correspond to a stored blank image. This embodiment can also be effected by using the shape and color of the test soil deposited over the query image. A blank image (or test soil image) would “correspond to” a stored blank image if the shape, and perhaps also the color, of the two images is the same within a predetermined criterion, or in an instance where the blank image is a bar-code or a QR code, if the scanned code and the stored code match. Such embodiments of disclosed methods have the advantage of precluding use of “third party” cleaning indicators that are not suitable for use in the washing machine system, or alerting an operator to mismatch of a cleaning indicator with a washing process it might not be suitable to evaluate. (For instance, when a cleaning indicator having a rubber substrate and stool as a test soil is inadvertently used in washing cycle for removing blood from glassware.)
Additionally or alternatively, such a method can be one comprising maintaining the washing machine system in a locked state if the query image detected by scanning it during or after the washing cycle does not correspond to a stored blank image or a stored query image.
A method for monitoring cleaning of articles can be one comprising:
In one embodiment, the stored blank or query image can be the image illustrated in
Devices and methods for scanning an image and determining its shape and color (i.e., “imaging”) are considered known in the art (e.g., U.S. Application Publication 2011/0291830), as are devices for reading and evaluating bar-codes or QR codes (e.g., U.S. Pat. No. 9,783,839 and U.S. Pat. No. 10,002,276; U.S. Pat. No. 9,739,764). Similarly, devices and methods for measuring the reflectance of a surface (e.g., U.S. Pat. No. 8,229,204) or for measuring fluorescence of a surface, both qualitatively and quantitatively, are also considered known in the art. Methods for qualitatively and quantitatively measuring fluorescence as a function of position in an image are also considered to be known in the art (e.g., U.S. Pat. No. 6,555,826). Quantitative methods including real-time correction of reflectance and fluorescence signals for fluctuation in scanning or excitation beam intensity and environmental scattering are also considered to be known in the art (e.g., U.S. Pat. No. 8,760,656 and U.S. Pat. No. 7,940,377).
Any of the examples or embodiments described herein may include various other features in addition to or in lieu of those described above. The teachings, expressions, embodiments, examples, etc., described herein should not be viewed in isolation relative to each other. Various suitable ways in which the teachings herein may be combined should be clear to those skilled in the art in view of the teachings herein.
Having shown and described exemplary embodiments of the subject matter contained herein, further adaptations of the methods and systems described herein may be accomplished by appropriate modifications without departing from the scope of the claims. In addition, where methods and steps described above indicate certain events occurring in certain order, it is intended that certain steps do not have to be performed in the order described but in any order as long as the steps allow the embodiments to function for their intended purposes. Therefore, to the extent there are variations of the invention, which are within the spirit of the disclosure or equivalent to the inventions found in the claims, it is the intent that this patent will cover those variations as well. Some such modifications should be apparent to those skilled in the art. For instance, the examples, embodiments, geometries, materials, dimensions, ratios, steps, and the like discussed above are illustrative. Accordingly, the claims should not be limited to the specific details of structure and operation set forth in the written description and drawings.
The present application is a divisional of U.S. application Ser. No. 17/440,142, filed Sep. 16, 2021, which is a national phase entry under 35 U.S.C. § 371 of International Application No. PCT/IB2020/052550, filed Mar. 19, 2020, which claims the benefit of the filing date of U.S. Provisional Patent Application No. 62/822, 179, filed Mar. 22, 2019, the disclosures of which are incorporated herein by reference.
| Number | Date | Country | |
|---|---|---|---|
| 62822179 | Mar 2019 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 17440142 | Sep 2021 | US |
| Child | 18786762 | US |
