Claims
- 1. A method of producing a cloned non-human mammalian embryo by nuclear transfer comprising introducing a contact-inhibited non-cycling mammalian donor cell in the G1 state or a nucleus thereof into an enucleated oocyte from a non-human mammal of the same species as the donor to form a nuclear transfer unit, implanting the nuclear transfer unit into the uterus of a surrogate mother and permitting the nuclear transfer unit to develop into the cloned non-human mammalian embryo.
- 2. A method of producing a cloned non-human mammalian embryo by nuclear transfer according to claim 1 wherein the non-human mammalian embryo is an ungulate.
- 3. A method of producing a cloned non-human mammalian embryo by nuclear transfer according to claim 2 wherein the ungulate is a pig.
- 4. A method of producing a cloned non-human mammalian embryo by nuclear transfer according to claim 3 wherein the pig is an H-transferase transgenic pig.
- 5. A method of producing a cloned non-human mammalian embryo by nuclear transfer according to claim 1 wherein the donor cell or donor cell nucleus is selected from the group consisting of epithelial cells, neural cells, epidermal cells, keratinocytes, hematopoietic cells, melanocytes, chondrocytes, B-lymphocytes, T-lymphocytes, erythrocytes, macrophages, monocytes, fibroblasts, muscle cells, and nuclei isolated therefrom.
- 6. A method of producing a cloned non-human mammalian embryo by nuclear transfer according to claim 1 wherein the non-human mammalian embryo is allowed to mature and become a neonate.
- 7. A method of producing a cloned non-human mammalian nuclear transfer unit by nuclear transfer comprising introducing a contact-inhibited non-cycling mammalian donor cell in the G1 state or a nucleus thereof into an enucleated oocyte from a non-human mammal of the same species as the donor to form a nuclear transfer unit.
- 8. A method of producing a cloned non-human mammalian nuclear transfer unit by nuclear transfer according to claim 7 wherein the species is an ungulate.
- 9. A method of producing a cloned non-human mammalian nuclear transfer unit by nuclear transfer according to claim 8 wherein the ungulate is a pig.
- 10. A method of producing a cloned non-human mammal by nuclear transfer according to claim 9 wherein the pig is an H-transferase transgenic pig.
- 11. A method of producing a cloned non-human mammalian nuclear transfer unit by nuclear transfer according to claim 7 wherein the donor cell or donor cell nucleus is selected from the group consisting of epithelial cells, neural cells, epidermal cells, keratinocytes, hematopoietic cells, melanocytes, chondrocytes, B-lymphocytes, T-lymphocytes, erythrocytes, macrophages, monocytes, fibroblasts, muscle cells, and nuclei isolated therefrom.
- 12. A method of producing a cloned non-human mammalian nuclear transfer unit by nuclear transfer according to claim 7 wherein the nuclear transfer unit is implanted into a surrogate mother.
- 13. A method of producing a cloned non-human mammalian nuclear transfer unit by nuclear transfer according to claim 12 wherein the nuclear transfer unit is becomes an embryo.
CROSS REFERENCE TO RELATED APPLICATION
[0001] This application claims priority to U.S. Provisional Serial No. 60/304,431 filed on Jul. 9, 2001, the contents of which are hereby incorporated by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60304431 |
Jul 2001 |
US |