Claims
- 1. An isolated recombinant DNA molecule comprising a nucleotide sequence which codes for a polypeptide which includes the conformational epitope of the 41/37 Kd protein of IBDV serotype I that is recognized by IBDV neutralizing monoclonal antibody MAb 17-82 and is capable of eliciting IBDV neutralizing antibodies in an avian host.
- 2. An isolated A recombinant DNA molecule comprising a nucleotide sequence which codes for a polypeptide which includes the conformational epitope encoded by the Ndel-Nael fragment of the IBDV genome sequence coding for the 41/37 KD protein of IBDV serotype I and is capable of eliciting IBDV neutralizing antibodies in an avian host.
- 3. A DNA molecule according to claim 1 or claim 2, wherein said nucleotide sequence codes for said polypeptide in the form of a fused polypeptide.
- 4. A DNA molecule according to claim 3, wherein said fused polypeptide includes portion(s) of the 3400 b.p. segment of IBDV coding for further polypeptides or proteins to correctly process said 41/37 Kd protein or fragment thereof.
- 5. A DNA molecule according to claim 3, wherein said fused polypeptide comprises said polypeptide which includes the conformational epitope of the 41/37 Kd protein of IBDV serotype I as the C-terminal sequence, and an additional polypeptide coded for by the DNA of a recombinant DNA cloning vehicle or vector as the N-terminal sequence fused thereto, such that the fused polypeptide is capable of eliciting IBDV neutralizing antibodies in an avian host.
- 6. An isolated recombinant DNA molecule comprising a nucleotide sequence substantially as shown in FIGS. 10A-10F, or a portion of said base sequence which codes for a polypeptide which includes the confirmational epitope of the 41/37 Kd protein of IBDV serotype I that is recognized by IBDV neutralizing monoclonal antibody MAb 17-82 and is capable of eliciting IBDV neutralizing antibodies in an avian host.
- 7. An isolated recombinant DNA molecule comprising a nucleotide sequence which codes for a serotype I IBDV 41/37 Kd polypeptide which retains a conformational neutralizing epitope of the native, intact IBDV virus that is recognized by IBDV neutralizing monoclonal antibody MAb 17-82 and is are absent from SDS-detergent-treated 41/37 Kd polypeptide.
- 8. A DNA molecule according to claim 7, wherein said nucleotide sequence codes for said polypeptide in the form of a fusion polypeptide with non-IBDV amino acid sequences encoded by a cloning vehicle or vector.
- 9. An isolated recombinant DNA molecule comprising a nucleotide sequence according to claim 1 or claim 2 wherein said sequence is under the control of an expression control sequence.
- 10. An isolated recombinant DNA cloning vehicle or vector capable of expressing a polypeptide which includes the conformational epitope of the 41/37 Kd protein of IBDV serotype I and is capable of eliciting IBDV neutralizing antibodies in an avian host, said cloning vehicle or vector having inserted therein a nucleotide sequence according to claim 1 or claim 2, said sequence being under the control of an expression control sequence.
- 11. A host cell containing a recombinant DNA molecule according to claim 9.
- 12. A host cell containing a recombinant DNA cloning vehicle or vector according to claim 10.
- 13. A host cell according to claim 11 which is E. coli.
- 14. A host cell according to claim 12, which is E. coli.
Priority Claims (2)
Number |
Date |
Country |
Kind |
PH 00815/85 |
May 1985 |
AUX |
|
PH 02118/85 |
Aug 1985 |
AUX |
|
Parent Case Info
This application is a Continuation of application Ser. No. 07/902,846, filed Jun. 23, 1992; which is a Continuation of application Ser. No. 07/018,941, filed as PCT/AU86/00156 May 5, 1986 abandoned.
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Number |
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Date |
Kind |
5350575 |
Azad et al. |
Sep 1994 |
|
5614409 |
Azad et al. |
Mar 1997 |
|
Non-Patent Literature Citations (3)
Entry |
Jagadish et al. 1990 Gene 95:179. |
MacNeadie et al 1990 Vaccine 8:549. |
Azad et al 1986 Virology 149:190. |
Continuations (2)
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Number |
Date |
Country |
Parent |
902846 |
Jun 1992 |
|
Parent |
18941 |
Jan 1987 |
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