Claims
- 1. A yeast from the genus Kluyveromyces transformed by a plasmid vector for expression of a heterologous protein in said yeast in which said vector is a circular plasmid comprising a portion of the DNA of the plasmid k.sub.1 containing at least the replication sequence, a DNA sequence encoding a heterologous gene, and a DNA sequence which insures expression of the said gene in the yeast.
- 2. A yeast as claimed in claim 1 wherein the heterologous gene is the URA.sub.3 gene of S. cerevisiae.
- 3. A method of preparing a protein encoded by a heterologous gene which comprises growing on a nutrient medium a yeast as claimed in claim 1.
Priority Claims (2)
Number |
Date |
Country |
Kind |
82 09564 |
Jun 1982 |
FRX |
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82 15114 |
Jun 1982 |
FRX |
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FIELD OF THE INVENTION
This application is a continuation of application Ser. No. 576,382, filed Jan. 17, 1984, filed as PCT FR83/00106 on June 1, 1983, published as WO83/04261 on Dec. 8, 1983, abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4418150 |
Gunge |
Nov 1983 |
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Non-Patent Literature Citations (5)
Entry |
Bach et al. (1979) Proceedings National Academy Sciences, USA vol. 76 pp. 386-390. |
de Louvencourt, L. et al. May 1983 J. Bacteriol 154:737. |
Struhl et al. (1979) P.N.A.S. 76:1035-1039. |
Das et al. (1982) Curr. Genet. 6:123-128. |
Niwa et al. (1981) J. Bact 148:988-990. |
Continuations (1)
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Number |
Date |
Country |
Parent |
576382 |
Jan 1984 |
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