Cloning of Hybrid Celery Using Bioreactor Technology

Information

• NSF Award
• 8760224

Owner

• Plant Genetics, Inc.

• Award Id

  8760224

• Award Effective Date

  2/1/1988 - 37 years ago

• Award Expiration Date

  8/31/1988 - 37 years ago

• Award Amount

  $ 47,024.00

• Award Instrument

  Standard Grant

This proposal describes research on bioreactor scale up of plant cell culture directed toward plant propagation. In Phase I research, the PI proposes to clone a breeder's cross hybrid of celery in a 1 to 4 liter bioreactor. Using this system, he proposes to identify the importance of agitation, aeration, and time in liquid culture on the yield and performance of developing celery somatic embryos. He further proposes to determine the level of singulation and synchronization of embryos resulting from regeneration in the bioreactor. A method for separating embryos from debris and immature embryos will be determined. Finally, several hundred somatic embryos will be regenerated and transplanted to the field. During Phase II research, he proposes to complete the field evaluation of plant variation as well as continue bioreactor studies. Development of these techniques could enable commercialization and delivery of hybrid crops where hybrid seed production methods do not currently exist.

• Program Officer

  Ritchie B. Coryell

• Min Amd Letter Date

  12/28/1987 - 37 years ago

• Max Amd Letter Date

  10/4/1988 - 37 years ago

• ARRA Amount

• Name

  Plant Genetics Inc

• City

  Davis

• State

  CA

• Country

  United States

• Address

  1930 Fifth Street

• Postal Code

  956164018

• Phone Number

  9167531400

• First Name

  David

• Last Name

  Stuart

• Start Date

  2/1/1988 12:00:00 AM

• Name

  Agriculture

• Code

  201000

• Name

  Life Science Biological

• Code

  61