Claims
- 1. A method for determining the concentration of ethanol in the bloodstream of a human who is suspected of having consumed ethanol, which comprises:
- (a) reacting saliva of said human with a dry, room-temperature-stable test device comprising an inert support pad containing alcohol oxidase, mannitol present in an amount sufficient to stabilize the alcohol oxidase, a salt that buffers in the range of pH 6-9, a substance having peroxidase activity, and a substance which will react with hydrogen peroxide to give a compound of changed color, whereby a change of color is obtained in the presence of ethanol; and
- (b) comparing any color so obtained to a standard to determine the ethanol concentration in the bloodstream of said human.
- 2. The method of claim 1, wherein said test device further comprises a polyamine polyacid metal chelating agent.
- 3. The method of claim 1, wherein said test device comprises alcohol oxidase in intimate admixture with from 300-2500 mg of said mannitol per 1000 enzyme units of alcohol oxidase.
- 4. The method of claim 3, wherein said test device further comprises from 2-50 mg of a polyamine polyacid metal chelating agent per 1000 enzyme units of alcohol oxidase.
Parent Case Info
This application is a continuation-in-part of application Ser. No. 513,505, now abandoned, filed July 12, 1983.
US Referenced Citations (8)
Non-Patent Literature Citations (2)
Entry |
Janssen et al, Biochimica et Biophysica Acta, vol. 151, 330-342, 1968. |
Majkic-Singh et al, Analytica Chimica Acta, vol. 115, 401-405, 1980. |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
513505 |
Jul 1983 |
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