Claims
- 1. A process for determining total lipid concentration in an animal fluid containing at least 1 mg. lipids per ml. which comprises:
- a. reacting a sample of the animal fluid with sulfuric acid in a container;
- b. subsequently adding directly to the animal fluid-sulfuric acid reaction product in said container, a phospho-vanillin reagent comprising--
- an aqueous solution of vanillin and phosphoric acid having a mole ratio of vanillin to phosphoric acid between 1.12 .times. 10.sup.-.sup.3 and 2.08 .times. 10.sup.-.sup.3 to form a chromogen without transferring a specific volume of the sulfuric acid-animal fluid mixture to a second container prior to adding the phospho-vanillin reagent, the mole ratio of phosphoric acid to sulfuric acid added to the animal fluid being between 9.5 and 55.5; and,
- c. measuring the light absorbence of said chromogen, the concentration of total lipids being determined from the measurement of light absorbence of said chromogen,
- said mole ratio of vanillin to phosphoric acid and said mole ratio of phosphoric acid to sulfuric acid enabling the addition of the phospho-vanillin reagent directly to the animal fluid-sulfuric acid reaction product and eliminating the necessity of transferring a specific volume of sulfuric acid-animal fluid mixture to a second container prior to adding the phospho-vanillin reagent.
- 2. The process of claim 1 wherein the animal fluid is blood serum.
- 3. The process of claim 1 wherein the animal fluid is human blood serum.
- 4. The process as set forth in claim 3 wherein the blood serum is mixed with sulfuric acid in the container and the mixture is incubated prior to the addition of the phospho-vanillin reagent.
- 5. The process as set forth in claim 4 further characterized in that the addition of the phospho-vanillin reagent directly to the blood serum-sulfuric acid reaction product enables lipid concentration in excess of 1000 mg/dl to be determined.
- 6. The process as set forth in claim 4 further characterized in that the addition of the phospho-vanillin reagent directly to the blood serum-sulfuric acid reaction product enables the total lipid concentration to be determined in blood serum samples having a volume less than 0.10 ml.
- 7. The process as set forth in claim 5 further characterized in that the addition of the phospho-vanillin reagent directly to the blood serum-sulfuric acid reaction product enables the total lipid concentration to be determined in blood serum samples having a volume less than 0.10 ml.
- 8. The process as set forth in claim 4 wherein the light absorbance is measured at 540 m.mu..
Parent Case Info
This is a continuation of application Ser. No. 372,267, filed June 21, 1973, now abandoned.
Non-Patent Literature Citations (3)
Entry |
frings & Dunn, Am. J. Clin. Path., v. 53, pp. 89-91 (1970). |
Frings et al., Clin. Chem., v. 18, pp. 673-674 (1972). |
Ratliff et al., Advances in Automated Analysis, vol. 1, pp. 101-107 (1972). |
Continuations (1)
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Number |
Date |
Country |
Parent |
372267 |
Jun 1973 |
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