Claims
- 1. A colorimetric sensor for detecting an analyte, comprising:
a receptor; and and a polymerized composition comprising at least one compound of the formula 4R3, R8, R13, R21, R24, R31 and R33 are independently C1-C20 alkyl; R4, R5, R7, R14, R16, R19, R20, R22, R25, and R32 are independently C1-C14 alkylene; R6, R15, R18, and R26 are independently C1-C14 alkylene, C2-C8 alkenylene, or C6-C13 arylene; R9 is C1-C14 alkylene or —NR34—; R10, R12, R27, and R29 are independently C1-C14 alkylene or (C1-C14 alkylene)-(C2-C8 arylene); R11 and R28 are independently C2-C30 alkynyl; R17 is an ester-activating group; R23 is C6-C13 arylene; R30 is C1-C14 alkylene or —NR36—; R34, and R36 are C1-C4 alkyl; p is 1-5; n is 1-20; wherein R1 and R2 are not the same; wherein the receptor is incorporated into the polymerized composition to form a transducer; and wherein the transducer exhibits a color change when contacted with an analyte.
- 2. The sensor of claim 1, wherein R1 is dodecyl or hexadecyl.
- 3. The sensor of claim 1, wherein R3 is undecyl or pentadecyl.
- 4. The sensor of claim 1, wherein R24 is methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, or dodecyl.
- 5. The sensor of claim 1, wherein R33 is dodecyl, tetradecyl, hexadecyl, or octadecyl.
- 6. The sensor of claim 1, wherein R4 is methylene, trimethylene, or tetramethylene.
- 7. The sensor of claim 1, wherein R5 is ethylene or trimethylene.
- 8. The sensor of claim 1, wherein R7, R16, R19, R20 and R25 are independently ethylene, trimethylene, tetramethylene, pentamethylene, hexamethylene, heptamethylene, octamethylene, or nonamethylene.
- 9. The sensor of claim 1, wherein R20 is methylene, trimethylene, or tetramethylene.
- 10. The sensor of claim 1, wherein R22 is ethylene, trimethylene, or tetramethylene.
- 11. The sensor of claim 1, wherein R32 is ethylene, trimethylene, tetramethylene, pentamethylene, or hexamethylene.
- 12. The sensor of claim 1, wherein R6, R15, R18, and R26 are independently ethylene, trimethylene, ethenylene, or phenylene.
- 13. The sensor of claim 1, wherein R8 and R31 are independently C1-C14 alkyl.
- 14. The sensor of claim 15, wherein R8 and R31 are independently butyl, pentyl or hexyl.
- 15. The sensor of claim 1, wherein R9 and R30 are independently methylene, NH—, —N(CH2CH3)—, or —N(CH3)—.
- 16. The sensor of claim 1, wherein R10, R12, R27, and R29 are independently methylene, ethylene, trimethylene, tetramethylene, —C(CH3)2—, —CH((CH2)1-4CH3)—, or —CH2-phenylene.
- 17. The sensor of claim 1, wherein R11 and R28 are independently C2-C30 alkynyl having at least two carbon-carbon triple bonds.
- 18. The sensor of claim 19, wherein R11 and R28 are independently —(CH2)8—C≡C—C≡C—(CH2)9CH3, or —(CH2)8—C≡C—C≡C—(CH2)11CH3.
- 19. The sensor of claim 1, wherein R13 is C1-C4 alkyl.
- 20. The sensor of claim 1, wherein R14 is C1-C4 alkylene.
- 21. The sensor of claim 1, wherein R17 is 2,5-dioxo-1-pyrrolidinyl.
- 22. The sensor of claim 1, wherein R23 is phenylene.
- 23. The sensor of claim 1, wherein n is 1-20, 3-17, 6-14, or 9-11.
- 24. The sensor of claim 1, wherein p is 1 or 2.
- 25. The sensor of claim 1 wherein R1 is
- 26. The sensor of claim 25, wherein R1 is
- 27. The sensor of claim 1, wherein the receptor is selected from the group consisting of phospholipid and glycerol.
- 28. The sensor of claim 1, wherein the transducer is dispersed in an aqueous solution.
- 29. The sensor of claim 1, wherein the transducer is coated on a substrate.
- 30. The sensor of claim 29, wherein the substrate exhibits a contact angle less than 50 degrees using methylene iodide.
- 31. The sensor of claim 30, wherein the substrate is selected from the group consisting of silica gel plate, paper, glass, textured photo paper, gloss photo paper, and microporous film.
- 32. The sensor of claim 1, wherein the receptor is integrated into the polymerized composition by physical mixing.
- 33. The sensor of claim 1, wherein the receptor is covalently bonded to the polymerized composition.
- 34. A method for the detection of an analyte, comprising
forming a calorimetric sensor, comprising a receptor and a polymerized composition of the formula 9R3, R8, R13, R21, R24, R31 and R33 are independently C1-C20 alkyl; R4, R5, R7, R14, R16, R19, R20, R22, R25, and R32 are independently C1-C14 alkylene; R6, R15, R18, and R26 are independently C1-C14 alkylene, C2-C8 alkenylene, or C6-C13 arylene; R9 is C1-C14 alkylene or —NR34—; R10, R12, R27, and R29 are independently C1-C14 alkylene or (C1-C14 alkylene)-(C2-C8 arylene); R11 and R28 are independently C2-C30 alkynyl; R17 is an ester-activating group; R23 is C6-C13 arylene; R30 is C1-C14 alkylene or —NR36—; R34, and R36 are C1-C4 alkyl; p is 1-5; n is 1-20; wherein R1 and R2 are not the same; wherein the receptor is incorporated into the polymerized composition to form a transducer capable of exhibiting a color change; contacting the sensor with an analyte; and observing a color change if the analyte is present.
- 35. The method of claim 34, wherein the transducer is dispersed in an aqueous solution.
- 36. The method of claim 34, wherein the transducer is coated on a substrate.
- 37. The method of claim 36, wherein the substrate exhibits a contact angle less than 50 degrees using methylene iodide.
- 38. The sensor of claim 30, wherein the substrate is selected from the group consisting of silica gel plate, paper, glass, textured photo paper, gloss photo paper, and microporous film.
- 39. The sensor of claim 34, wherein the receptor is integrated into the polymerized composition by physical mixing.
- 40. The sensor of claim 34, wherein the receptor is covalently bonded to the polymerized composition.
- 41. A method for the detection of an analyte, comprising
forming a colorimetric sensor, comprising a receptor and a polymerized composition of the formula 10R3, R8, R13, R21, R24, R31 and R33 are independently C1-C20 alkyl; R4, R5, R7, R14, R16, R19, R20, R22, R25, and R32 are independently C1-C14 alkylene; R6, R15, R18, and R26 are independently C1-C14 alkylene, C2-C8 alkenylene, or C6-C13 arylene; R9 is C1-C14 alkylene or —NR34—; R10, R12, R27, and R29 are independently C1-C14 alkylene or(C1-C14 alkylene)-(C2-C8 arylene); R11 and R28 are independently C2-C30 alkynyl; R17 is an ester-activating group; R23 is C6-C13 arylene; R30 is C1-C14 alkylene or —NR36—; R34, and R36 are C1-C4 alkyl; p is 1-5; n is 1-20; wherein R1 and R2 are not the same; and wherein the receptor is incorporated into the polymerized composition to form a transducer capable of exhibiting a color change; contacting the transducer with an analyte; contacting the transducer with a probe that has an affinity for both the analyte and the receptor; and observing no color change if the analyte is present.
- 42. The method of claim 41, wherein the probe and analyte are combined to form a mixture before contacting the transducer.
- 43. The method of claim 41, wherein the transducer is dispersed in an aqueous solution.
- 44. The method of claim 41, wherein the transducer is coated on a substrate.
- 45. The method of claim 44, wherein the substrate exhibits a contact angle less than 50 degrees using methylene iodide.
- 46. The method of claim 45, wherein the substrate is selected from the group consisting of silica gel plate, paper, glass, textured photo paper, gloss photo paper, and microporous film.
- 47. The method of claim 41, wherein the receptor is integrated into the polymerized composition by physical mixing.
- 48. The method of claim 41, wherein the receptor is covalently bonded to the polymerized composition.
- 49. The method of claim 41, wherein the probe comprises a membrane disrupting peptide.
- 50. The method of claim 49, wherein the probe is selected from the group consisting of alamethicin, magainin, gramicidin, polymyxin B sulfate, and melittin.
- 51. The method of claim 41, wherein the analyte is selected from the group consisting of gram-negative bacteria and endotoxin.
- 52. A disposable kit for detecting the presence of an analyte, comprising:
one or more transducers comprising a receptor and a polymerized composition of the formula 11R3, R8, R13, R21, R24, R31 and R33 are independently C1-C20 alkyl; R4, R5, R7, R14, R16, R19, R20, R22, R25, and R32 are independently C1-C14 alkylene; R6, R15, R18, and R26 are independently C1-C14 alkylene, C2-C8 alkenylene, or C6-C13 arylene; R9 is C1-C14 alkylene or —NR34—; R10, R12, R27, and R29 are independently C1-C14 alkylene or (C1-C14 alkylene)-(C2-C8 arylene); R11 and R28 are independently C2-C30 alkynyl; R17 is an ester-activating group; R23 is C6-C13 arylene; R30 is C1-C14 alkylene or —NR36—; R34, and R36 are C1-C4 alkyl; p is 1-5; n is 1-20; wherein R1 and R2 are not the same; and wherein the receptor is integrated with the polymerized composition to form a transducer capable of exhibiting a color change; and a means for contacting the transducer with an analyte.
- 53. The kit of claim 52, further comprising one or more probes with an affinity to the receptor and one or more analytes; wherein the probe is physically separated from the transducer until the kit is used.
- 54. The kit of claim 52, wherein one or more transducers are dispersed in an aqueous solution.
- 55. The kit of claim 52, wherein one or more transducers are coated on a substrate to form an array.
- 56. The method of claim 55, wherein the substrate exhibits a contact angle less than 50 degrees using methylene iodide.
- 57. The sensor of claim 56, wherein the substrate is selected from the group consisting of silica gel plate, paper, glass, textured photo paper, gloss photo paper, and microporous film.
- 58. The kit of claim 52, wherein the receptor is integrated into the polymerized composition by physical mixing.
- 59. The kit of claim 52, wherein the receptor is covalently bonded to the polymerized composition.
- 60. A method of detecting the presence of bacteria in a body fluid, comprising the step of contacting the sensor of claim 1 with a body fluid.
- 61. The method of claim 60, wherein the body fluid is selected from the group consisting of urine, mucus, wound exudate, and blood.
- 62. The calorimetric sensor of claim 1, further comprising a diagnostic test for detectingleukocytes.
- 63. A method of detecting the presence of bacteria in a body fluid, comprising the step of contacting the sensor of claim 62 with a body fluid.
- 64. The method of claim 63, wherein the body fluid is selected from the group consisting of urine, mucus, wound exudate, and blood.
- 65. A medical article, comprising a wound dressing and the colorimetric sensor of claim 1.
- 66. The medical article of claim 65, wherein the colorimetric sensor is connected to the wound bed by a fluid control system.
- 67. The medical article of claim 66 wherein the fluid control system is a microfluidic system.
- 68. A method of detecting the presence of bacteria, the method comprising the step of inserting the colorimetric sensor of claim 1 in a wound dressing.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 10/325,801, filed Dec. 19, 2002.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
10325801 |
Dec 2002 |
US |
Child |
10738573 |
Dec 2003 |
US |