Patent Application
20200101049
The present disclosure relates generally to medical methods and devices. More particularly, the present disclosure relates to medical methods and kits for distributing temsirolimus in the tissue surrounding a blood vessel. Further, the present disclosure relates to medical methods and kits for distributing temsirolimus in combination with a glucocorticoid in the tissue surrounding a blood vessel.
Blockages can form in blood vessels under various disease conditions. In atherosclerosis, the narrowing of arteries in the body, particularly in the heart, legs, carotid and renal anatomy, can lead to tissue ischemia from lack of blood flow. Mechanical revascularization methods, such as balloon angioplasty, atherectomy, stenting, or surgical endarterectomy, are used to open the blood vessel and to improve blood flow to downstream tissues. Unfortunately, mechanical revascularization can lead to an injury cascade that causes the blood vessel to stiffen and vessel walls to thicken with a scar-like tissue, which can reduce the blood flow and necessitate another revascularization procedure. There is a great desire to reduce the vessel stiffening and thickening following mechanical revascularization to maintain or improve the patency of the blood vessel.
The present disclosure provides methods, devices, and compositions for distributing a combination of a cell division inhibitor and a glucocorticoid into a tissue surrounding a blood vessel for treating vascular diseases.
Provided herein are pharmaceutical compositions comprising temsirolimus and a glucocorticoid, or their pharmaceutically acceptable salts thereof. Further provided herein are pharmaceutical compositions wherein the glucocorticoid is dexamethasone or a pharmaceutically acceptable salt thereof. Further provided herein are pharmaceutical compositions wherein the ratio (by weight) of temsirolimus to the glucocorticoid, or vice versa, is between 10:1 to 1:1. Further provided herein are pharmaceutical compositions wherein the composition is in an injectable dosage form. Further provided herein are pharmaceutical compositions wherein the composition further comprises at least one pharmaceutically acceptable excipient. Further provided herein are pharmaceutical compositions wherein the concentration of dexamethasone is 1.0-8.0 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of dexamethasone is about 3.2 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of dexamethasone is less than 4.0 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of temsirolimus is 0.01-2.0 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of temsirolimus is 0.05-0.5 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of temsirolimus is about 0.1 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of temsirolimus is about 0.4 mg/mL. Further provided herein are pharmaceutical compositions for use in treating restenosis. Further provided herein are pharmaceutical compositions for use in treating restenosis below the knee. Further provided herein are pharmaceutical compositions for use in treating restenosis above the knee. Further provided herein are pharmaceutical compositions for use in treating restenosis in a below-knee popliteal vessel or tibial vessel.
Provided herein are injectable compositions comprising temsirolimus or a pharmaceutically acceptable salt thereof, dexamethasone or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery to a coronary vessel. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery to a coronary artery. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery below the knee. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery above the knee. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery to a below-knee popliteal or tibial vessel. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery to a femoral vessel. Further provided herein are injectable compositions wherein the therapeutically effective amount of temsirolimus is about 1 μg to 50 mg. Further provided herein are injectable compositions wherein the therapeutically effective amount of temsirolimus is about 10 μg to 20 mg. Further provided herein are injectable compositions wherein the therapeutically effective amount of temsirolimus is about 100 μg to 15 mg. Further provided herein are injectable compositions wherein the therapeutically effective amount of temsirolimus is about 100 μg to 5 mg. Further provided herein are injectable compositions wherein the therapeutically effective amount is determined by a longitudinal length of a target diseased artery. Further provided herein are injectable compositions wherein the therapeutically effective amount of dexamethasone is about 0.8 mg to 8 mg per cm of longitudinal length of a disease site in the blood vessel. Further provided herein are injectable compositions wherein the therapeutically effective amount of dexamethasone is about 0.05 mg to 10 mg per cm of longitudinal length of a disease site in the blood vessel and the therapeutically effective amount of temsirolimus is about 0.005 mg to 5 mg per cm of longitudinal length of a disease site in the blood vessel. Further provided herein are injectable compositions wherein the therapeutically effective amount of dexamethasone is about 0.1 mg to 2 mg per cm of longitudinal length of a disease site in the blood vessel and the therapeutically effective amount of temsirolimus is about 0.025 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel. Further provided herein are injectable compositions wherein the injection volume of the composition is about 0.01 mL to about 50 mL. Further provided herein are injectable compositions wherein the injection volume of the composition is about 0.5 mL to about 20 mL. Further provided herein are injectable compositions wherein the injection concentration of temsirolimus is about 0.01 mg/mL to about 2.0 mg/mL. Further provided herein are injectable compositions wherein the injection concentration of temsirolimus is about 0.1 mg/mL to about 0.5 mg/mL. Further provided herein are injectable compositions wherein the injection concentration of temsirolimus is about 0.4 mg/mL. Further provided herein are injectable compositions wherein the pharmaceutically acceptable excipient is 0.9% sodium chloride injection USP, dehydrated alcohol, dl-alpha tocopherol, anhydrous citric acid, polysorbate 80, polyethylene glycol 400, propylene glycol, or a combination thereof. Further provided herein are injectable compositions for use in treating restenosis. Further provided herein are injectable compositions for use in treating restenosis below the knee. Further provided herein are injectable compositions for use in treating restenosis above the knee. Further provided herein are injectable compositions for use in treating restenosis in a below-knee popliteal vessel or tibial vessel.
Provided herein are methods of treating a vascular disease in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a pharmaceutical composition described herein, wherein the composition is administered by direct injection to a disease site. Further provided herein are methods of treating a vascular disease wherein the composition is injected though a catheter with a needle. Further provided herein are methods of treating a vascular disease wherein the composition is injected distal or proximal to the disease site. Further provided herein are methods of treating a vascular disease wherein the composition is injected at least about 2 cm away from the disease site. Further provided herein are methods of treating a vascular disease wherein the composition is injected at or adjacent to the disease site. Further provided herein are methods of treating a vascular disease wherein the composition is administered by injection into the blood vessel. Further provided herein are methods of treating a vascular disease wherein the composition is injected into an adventitial tissue surrounding a blood vessel. Further provided herein are methods of treating a vascular disease wherein the composition is injected into a perivascular tissue surrounding the blood vessel. Further provided herein are methods of treating a vascular disease wherein the blood vessel is an artery. Further provided herein are methods of treating a vascular disease wherein the blood vessel is a vein. Further provided herein are methods of treating a vascular disease wherein the artery is a coronary artery or a peripheral artery. Further provided herein are methods of treating a vascular disease wherein the artery is selected from the group consisting of renal artery, cerebral artery, pulmonary artery, and artery in the leg. Further provided herein are methods of treating a vascular disease wherein the artery is below the knee. Further provided herein are methods of treating a vascular disease wherein the blood vessel is below-knee popliteal vessel or tibial vessel. Further provided herein are methods of treating a vascular disease wherein the composition is injected into a blood vessel wall. Further provided herein are methods of treating a vascular disease wherein the composition is injected into a tissue surrounding the blood vessel wall. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of temsirolimus is about 1 μg to 50 mg. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of temsirolimus is about 10 μg to 20 mg. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of temsirolimus is about 100 μg to 15 mg. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of temsirolimus is about 100 μg to 5 mg. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of dexamethasone is about 0.8 mg to 8 mg per cm of longitudinal length of the disease site in the blood vessel. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of dexamethasone is about 0.05 mg to 10 mg per cm of longitudinal length of the disease site in the blood vessel and the therapeutically effective amount of temsirolimus is about 0.005 mg to 5 mg per cm of longitudinal length of the disease site in the blood vessel. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of dexamethasone is about 0.1 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel and the therapeutically effective amount of temsirolimus is about 0.025 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel. Further provided herein are methods of treating a vascular disease wherein the injection volume of the composition is about 0.01 mL to about 50 mL. Further provided herein are methods of treating a vascular disease wherein the injection volume of the composition is about 0.5 mL to about 20 mL. Further provided herein are methods of treating a vascular disease wherein the injection concentration of temsirolimus is about 0.01 mg/mL to about 2.0 mg/mL. Further provided herein are methods of treating a vascular disease wherein the injection concentration of temsirolimus is about 0.1 mg/mL to about 0.4 mg/mL. Further provided herein are methods of treating a vascular disease wherein the injection concentration of temsirolimus is about 0.4 mg/mL. Further provided herein are methods of treating a vascular disease wherein the injection concentration of temsirolimus is about 0.1 mg/mL. Further provided herein are methods of treating a vascular disease wherein 12 months after administration of the pharmaceutical composition, vessel cross-sectional area at the disease site has decreased no more than 60%, when compared to vessel cross-sectional area at the disease site at the time of administration. Further provided herein are methods of treating a vascular disease wherein 12 months after administration of the pharmaceutical composition, vessel cross-sectional area at the disease site has decreased no more than 50%, when compared to vessel cross-sectional area at the disease site at the time of administration. Further provided herein are methods of treating a vascular disease wherein 12 months after administration of the pharmaceutical composition, vessel cross-sectional area at the disease site has decreased no more than 30%, when compared to vessel cross-sectional area at the disease site at the time of administration. Further provided herein are methods of treating a vascular disease wherein the subject is human. Further provided herein are methods of treating a vascular disease wherein the vascular disease is angina, myocardial infarction, congestive heart failure, cardiac arrhythmia, peripheral artery disease, claudication, or critical limb ischemia. Further provided herein are methods of treating a vascular disease wherein the vascular disease is atherosclerosis, bypass graft failure, transplant vasculopathy, vascular restenosis, or in-stent restenosis. Further provided herein are methods of treating a vascular disease wherein treating using the pharmaceutical composition results in a decrease in apoptosis at the disease site relative to treating using a pharmaceutical composition comprising either temsirolimus or a glucocorticoid. Further provided herein are methods of treating a vascular disease wherein treating using the pharmaceutical composition results in a decrease in necrosis at the disease site relative to treating using a pharmaceutical composition comprising either temsirolimus or a glucocorticoid.
Provided herein are methods of treating a vascular disease in an individual in need thereof, the method comprising administering to the subject a therapeutically effective amount of a first pharmaceutical composition and a second pharmaceutical composition, wherein the first pharmaceutical composition comprises temsirolimus, and the second pharmaceutical composition comprises a glucocorticoid, wherein the first composition and the second composition are each administered by injection.
Provided herein are methods of treating a peripheral artery disease in a human subject in need thereof, the method comprising administering to the human subject a therapeutically effective amount of a pharmaceutical composition comprising temsirolimus or pharmaceutically acceptable salt thereof, and a glucocorticoid or pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, wherein the composition is administered by direct injection to or near a disease site in a wall of a peripheral artery via a laterally extending injection needle of a catheter advanced through vasculature of the human subject. Further provided herein are methods wherein the composition further comprises a contrast medium for visualizing the injection.
Provided herein are injectable compositions comprising temsirolimus and a glucocorticoid, or pharmaceutically acceptable salts thereof, and a pharmaceutically acceptable excipient for use in treating a vascular disease of a human subject, wherein the composition is suitable for adventitial delivery to a peripheral artery, wherein the composition is suitable for direct injection to a vascular disease site in a wall of the peripheral artery via a laterally extending needle from a catheter advanced through vasculature of the human subject. Further provided herein are methods wherein the composition further comprises a contrast medium for visualizing the injection.
Provided herein are pharmaceutical compositions comprising a cell division inhibitor and a glucocorticoid, or their pharmaceutically acceptable salts thereof. Further provided herein are pharmaceutical compositions wherein the glucocorticoid is dexamethasone or a pharmaceutically acceptable salt thereof. Further provided herein are pharmaceutical compositions wherein the ratio (by weight) of a cell division inhibitor to the glucocorticoid, or vice versa, is between 10:1 to 1:1. Further provided herein are pharmaceutical compositions wherein the composition is in an injectable dosage form. Further provided herein are pharmaceutical compositions wherein the composition further comprises at least one pharmaceutically acceptable excipient. Further provided herein are pharmaceutical compositions wherein the concentration of dexamethasone is 1.0-8.0 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of dexamethasone is about 3.2 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of dexamethasone is less than 4.0 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of a cell division inhibitor is 0.01-2.0 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of a cell division inhibitor is 0.05-0.5 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of a cell division inhibitor is about 0.1 mg/mL. Further provided herein are pharmaceutical compositions wherein the concentration of a cell division inhibitor is about 0.4 mg/mL. Further provided herein are pharmaceutical compositions for use in treating restenosis. Further provided herein are pharmaceutical compositions for use in treating restenosis below the knee. Further provided herein are pharmaceutical compositions for use in treating restenosis above the knee. Further provided herein are pharmaceutical compositions for use in treating restenosis in a below-knee popliteal vessel or tibial vessel. Further provided herein are pharmaceutical compositions wherein the cell division inhibitor is paclitaxel.
Provided herein are injectable compositions comprising a cell division inhibitor or a pharmaceutically acceptable salt thereof, dexamethasone or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery to a coronary vessel. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery to a coronary artery. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery below the knee. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery above the knee. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery to a below-knee popliteal or tibial vessel. Further provided herein are injectable compositions wherein the composition is suitable for adventitial delivery to a femoral vessel. Further provided herein are injectable compositions wherein the therapeutically effective amount of a cell division inhibitor is about 1 μg to 50 mg. Further provided herein are injectable compositions wherein the therapeutically effective amount of a cell division inhibitor is about 10 μg to 20 mg. Further provided herein are injectable compositions wherein the therapeutically effective amount of a cell division inhibitor is about 100 μg to 15 mg. Further provided herein are injectable compositions wherein the therapeutically effective amount of a cell division inhibitor is about 100 μg to 5 mg. Further provided herein are injectable compositions wherein the therapeutically effective amount is determined by a longitudinal length of a target diseased artery. Further provided herein are injectable compositions wherein the therapeutically effective amount of dexamethasone is about 0.8 mg to 8 mg per cm of longitudinal length of a disease site in the blood vessel. Further provided herein are injectable compositions wherein the therapeutically effective amount of dexamethasone is about 0.05 mg to 10 mg per cm of longitudinal length of a disease site in the blood vessel and the therapeutically effective amount of a cell division inhibitor is about 0.005 mg to 5 mg per cm of longitudinal length of a disease site in the blood vessel. Further provided herein are injectable compositions wherein the therapeutically effective amount of dexamethasone is about 0.1 mg to 2 mg per cm of longitudinal length of a disease site in the blood vessel and the therapeutically effective amount of a cell division inhibitor is about 0.025 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel. Further provided herein are injectable compositions wherein the injection volume of the composition is about 0.01 mL to about 50 mL. Further provided herein are injectable compositions wherein the injection volume of the composition is about 0.5 mL to about 20 mL. Further provided herein are injectable compositions wherein the injection concentration of a cell division inhibitor is about 0.01 mg/mL to about 2.0 mg/mL. Further provided herein are injectable compositions wherein the injection concentration of a cell division inhibitor is about 0.1 mg/mL to about 0.5 mg/mL. Further provided herein are injectable compositions wherein the injection concentration of a cell division inhibitor is about 0.4 mg/mL. Further provided herein are injectable compositions wherein the pharmaceutically acceptable excipient is 0.9% sodium chloride injection USP, dehydrated alcohol, dl-alpha tocopherol, anhydrous citric acid, polysorbate 80, polyethylene glycol 400, propylene glycol, or a combination thereof. Further provided herein are injectable compositions for use in treating restenosis. Further provided herein are injectable compositions for use in treating restenosis below the knee. Further provided herein are injectable compositions for use in treating restenosis above the knee. Further provided herein are injectable compositions for use in treating restenosis in a below-knee popliteal vessel or tibial vessel. Further provided herein are injectable compositions wherein the cell division inhibitor is paclitaxel.
Provided herein are methods of treating a vascular disease in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a pharmaceutical composition described herein, wherein the composition is administered by direct injection to a disease site. Further provided herein are methods of treating a vascular disease wherein the composition is injected though a catheter with a needle. Further provided herein are methods of treating a vascular disease wherein the composition is injected distal or proximal to the disease site. Further provided herein are methods of treating a vascular disease wherein the composition is injected at least about 2 cm away from the disease site. Further provided herein are methods of treating a vascular disease wherein the composition is injected at or adjacent to the disease site. Further provided herein are methods of treating a vascular disease wherein the composition is administered by injection into a blood vessel. Further provided herein are methods of treating a vascular disease wherein the composition is injected into an adventitial tissue surrounding a blood vessel. Further provided herein are methods of treating a vascular disease wherein the composition is injected into a perivascular tissue surrounding the blood vessel. Further provided herein are methods of treating a vascular disease wherein the blood vessel is an artery. Further provided herein are methods of treating a vascular disease wherein the blood vessel is a vein. Further provided herein are methods of treating a vascular disease wherein the artery is a coronary artery or a peripheral artery. Further provided herein are methods of treating a vascular disease wherein the artery is selected from the group consisting of renal artery, cerebral artery, pulmonary artery, and artery in the leg. Further provided herein are methods of treating a vascular disease wherein the artery is below the knee. Further provided herein are methods of treating a vascular disease wherein the blood vessel is below-knee popliteal vessel or tibial vessel. Further provided herein are methods of treating a vascular disease wherein the composition is injected into a blood vessel wall. Further provided herein are methods of treating a vascular disease wherein the composition is injected into a tissue surrounding the blood vessel wall. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of a cell division inhibitor is about 1 μg to 50 mg. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of a cell division inhibitor is about 10 μg to 20 mg. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of a cell division inhibitor is about 100 μg to 15 mg. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of a cell division inhibitor is about 100 μg to 5 mg. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of dexamethasone is about 0.8 mg to 8 mg per cm of longitudinal length of the disease site in the blood vessel. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of dexamethasone is about 0.05 mg to 10 mg per cm of longitudinal length of the disease site in the blood vessel and the therapeutically effective amount of a cell division inhibitor is about 0.005 mg to 5 mg per cm of longitudinal length of the disease site in the blood vessel. Further provided herein are methods of treating a vascular disease wherein the therapeutically effective amount of dexamethasone is about 0.1 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel and the therapeutically effective amount of a cell division inhibitor is about 0.025 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel. Further provided herein are methods of treating a vascular disease wherein the injection volume of the composition is about 0.01 mL to about 50 mL. Further provided herein are methods of treating a vascular disease wherein the injection volume of the composition is about 0.5 mL to about 20 mL. Further provided herein are methods of treating a vascular disease wherein the injection concentration of a cell division inhibitor is about 0.01 mg/mL to about 2.0 mg/mL. Further provided herein are methods of treating a vascular disease wherein the injection concentration of a cell division inhibitor is about 0.1 mg/mL to about 0.4 mg/mL. Further provided herein are methods of treating a vascular disease wherein the injection concentration of a cell division inhibitor is about 0.4 mg/mL. Further provided herein are methods of treating a vascular disease wherein the injection concentration of a cell division inhibitor is about 0.1 mg/mL. Further provided herein are methods of treating a vascular disease wherein 12 months after administration of the pharmaceutical composition, vessel cross-sectional area at the disease site has decreased no more than 60%, when compared to vessel cross-sectional area at the disease site at the time of administration. Further provided herein are methods of treating a vascular disease wherein 12 months after administration of the pharmaceutical composition, vessel cross-sectional area at the disease site has decreased no more than 50%, when compared to vessel cross-sectional area at the disease site at the time of administration. Further provided herein are methods of treating a vascular disease wherein 12 months after administration of the pharmaceutical composition, vessel cross-sectional area at the disease site has decreased no more than 30%, when compared to vessel cross-sectional area at the disease site at the time of administration. Further provided herein are methods of treating a vascular disease wherein the subject is human. Further provided herein are methods of treating a vascular disease wherein the vascular disease is angina, myocardial infarction, congestive heart failure, cardiac arrhythmia, peripheral artery disease, claudication, or critical limb ischemia. Further provided herein are methods of treating a vascular disease wherein the vascular disease is atherosclerosis, bypass graft failure, transplant vasculopathy, vascular restenosis, or in-stent restenosis. Further provided herein are methods of treating a vascular disease wherein treating using the pharmaceutical composition results in a decrease in apoptosis at the disease site relative to treating using a pharmaceutical composition comprising either a cell division inhibitor or a glucocorticoid. Further provided herein are methods of treating a vascular disease wherein treating using the pharmaceutical composition results in a decrease in necrosis at the disease site relative to treating using a pharmaceutical composition comprising either a cell division inhibitor or a glucocorticoid. Further provided herein are methods wherein the cell division inhibitor is paclitaxel.
Provided herein are methods of treating a vascular disease in an individual in need thereof, the method comprising administering to the subject a therapeutically effective amount of a first pharmaceutical composition and a second pharmaceutical composition, wherein the first pharmaceutical composition comprises a cell division inhibitor, and the second pharmaceutical composition comprises a glucocorticoid, wherein the first composition and the second composition are each administered by injection. Further provided herein are methods wherein the cell division inhibitor is paclitaxel.
Provided herein are methods of treating a peripheral artery disease in a human subject in need thereof, the method comprising administering to the human subject a therapeutically effective amount of a pharmaceutical composition comprising a cell division inhibitor or pharmaceutically acceptable salt thereof, and a glucocorticoid or pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, wherein the composition is administered by direct injection to or near a disease site in a wall of a peripheral artery via a laterally extending injection needle of a catheter advanced through vasculature of the human subject. Further provided herein are methods wherein the composition further comprises a contrast medium for visualizing the injection. Further provided herein are methods wherein the cell division inhibitor is paclitaxel.
Provided herein are injectable compositions comprising a cell division inhibitor and a glucocorticoid, or pharmaceutically acceptable salts thereof, and a pharmaceutically acceptable excipient for use in treating a vascular disease of a human subject, wherein the composition is suitable for adventitial delivery to a peripheral artery, wherein the composition is suitable for direct injection to a vascular disease site in a wall of the peripheral artery via a laterally extending needle from a catheter advanced through vasculature of the human subject. Further provided herein are methods wherein the composition further comprises a contrast medium for visualizing the injection. Further provided herein are injectable compositions wherein the cell division inhibitor is paclitaxel.
All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference.
The novel features of the present disclosure are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present disclosure will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the present disclosure are utilized, and the accompanying drawings of which:
Provided herein are methods, devices, and compositions for the treatment of cardiovascular diseases, such as restenosis. Various aspects of the methods, devices, and compositions comprise combinations of an mTOR inhibitor (such as temsirolimus) or a cell division inhibitor (such as paclitaxel) and a glucocorticoid (such as dexamethasone), or their pharmaceutically acceptable salts. In some cases, the compositions are administered by direct injection to the disease site.
Blockages form in blood vessels under various disease conditions. Atherosclerosis, which causes the narrowing, or stenosis, of arteries in the body, particularly in the heart, legs, carotid, and renal anatomy, leads to tissue ischemia from lack of blood flow. In some instances, atherosclerosis in the coronary arteries causes myocardial infarction, commonly referred to as a heart attack, which can be immediately fatal, or even if survived, causes damage to the heart which can incapacitate the patient. Other coronary diseases include congestive heart failure, vulnerable or unstable plaque, and cardiac arrhythmias, which cause death and incapacitation. In addition, peripheral artery disease (PAD), where the arteries in peripheral tissues narrow, most commonly affects the leg, renal, and carotid arteries. In some instances, blood clots and thrombus in the peripheral vasculature occlude peripheral blood flow, leading to tissue and organ necrosis. Some patients with PAD experience critical limb ischemia that results in ulcers and some instances requires amputation in the worst cases. In some instances, PAD in a renal artery causes renovascular hypertension, and clots in the carotid artery embolize and travel to the brain, potentially causing ischemic stroke.
To improve blood flow to downstream tissues, various revascularization methods are used to bypass or open the artery. In some instances, artery bypass surgery is an effective treatment for stenosed, or narrowed, arteries resulting from atherosclerosis and other causes, but it is a highly invasive procedure, expensive, requires substantial hospital and recovery time. In some instances, mechanical revascularization methods with balloon angioplasty, atherectomy, stenting, or surgical endarterectomy are used to open, or dilate, the artery. For example, percutaneous transluminal angioplasty (PTA), commonly referred to as balloon angioplasty, is less invasive, less traumatic, and significantly less expensive than bypass surgery. In addition, the effectiveness of balloon angioplasty has improved with the introduction of stenting which involves the placement of a scaffold structure within the artery which has been treated by balloon angioplasty. The stent inhibits abrupt re-closure of the artery and has some benefit in reducing subsequent restenosis resulting from hyperplasia. By salvaging blood vessels rather than bypassing them, more options are left available to physicians in the further treatment of the disease.
Unfortunately, in some instances, mechanical revascularization procedures lead to an injury cascade that causes the artery to stiffen and arterial walls to thicken with a scar-like tissue, known as neointimal hyperplasia. In some instances, not only does the inner wall of the artery (i.e., the intima) thicken and stiffen in response to the injury cascade, but the media (i.e., the middle tissue layer of the wall) and the adventitia (i.e., the outer layer of the wall) can thicken and stiffen as well. The thickening (or hyperplasia) and the stiffening (or sclerosis), reduces the blood flow to tissues distal to the affected site. As a result, patients who have undergone mechanical revascularization procedures in some instances suffer from a high incidence of restenosis resulting from hyperplasia. Restenosis, or recurrence of stenosis or narrowing, of the blood vessel may necessitate another revascularization procedure to the affected area again.
Inflammatory restenosis and reocclusion in some cases occurs due to hyperplasia and sclerosis. The resolution of an inflammatory impetus in some instances leads to a stiff, narrow vessel or a functioning artery with vasomotor actions (including the ability to dilate or constrict based on signals received from the body's endocrine system. In order to maintain long-term vascular patency, the vessel must be allowed to heal without sclerosis or hyperplasia.
Some embodiments herein describe compositions and methods for reducing the buildup of sclerosis and hyperplasia following mechanical revascularization.
In some embodiments, implanting stents coated with anti-proliferative drugs reduces the occurrence of hyperplasia. For example, mechanical endovascular revascularization alone leads to patency (the binary measure of vessel openness, typically greater than 50% in diameter compared to adjacent non-diseased vessel) rates of 33-55% at one year and 20-50% at two years, while drug-coated balloons and adventitial drug delivery have shown an ability to improve patency to better than 80% at one year and 65-70% at 2 years.
Described herein are pharmaceutical compositions comprising a cell division inhibitor, a glucocorticoid (such as dexamethasone or a pharmaceutically acceptable salt thereof), and a pharmaceutically acceptable excipient. Some embodiments herein describe a pharmaceutical composition comprising a microtubule stabilizer (such as a taxane or a pharmaceutically acceptable salt thereof), a glucocorticoid (such as dexamethasone or a pharmaceutically acceptable salt thereof), and a pharmaceutically acceptable excipient. Some embodiments herein describe a pharmaceutical composition comprising an mTOR inhibitor (such as temsirolimus or a pharmaceutically acceptable salt thereof), a glucocorticoid (such as dexamethasone or a pharmaceutically acceptable salt thereof), and a pharmaceutically acceptable excipient. Other embodiments herein describe a pharmaceutical composition comprising a taxane (such as paclitaxel or a pharmaceutically acceptable salt thereof), a glucocorticoid (such as dexamethasone or a pharmaceutically acceptable salt thereof), and a pharmaceutically acceptable excipient. In some embodiments, the pharmaceutical compositions are useful for treating the diseases and condition described herein (e.g., reducing the buildup of sclerosis and hyperplasia following mechanical revascularization).
mTOR Inhibitors
In certain embodiments, the pharmaceutical compositions described herein comprise an mTOR inhibitor, such as a -limus drug. In some cases, the mTOR inhibitor is sirolimus, everolimus, zotarolimus, deforolimus, biolimus, temsirolimus, or combinations thereof. The pharmaceutical compositions disclosed herein in some instances comprise temsirolimus or its pharmaceutically acceptable salts thereof. In some embodiments, the mTOR inhibitor is Torisel®.
The pharmaceutical compositions disclosed herein in some instances comprise temsirolimus or its pharmaceutically acceptable salts thereof. In some instances, the pharmaceutical compositions comprise pharmaceutically acceptable excipients. In some instances, the pharmaceutical compositions further comprise excipients including 0.9% sodium chloride injection USP, dehydrated alcohol, dl-alpha tocopherol, anhydrous citric acid, polysorbate 80, polyethylene glycol 400, propylene glycol, or a combination thereof. In some instances, the pharmaceutical compositions comprise nanoparticle formulations. In some instances, the pharmaceutical compositions further comprise excipients including albumin. In some instances, the pharmaceutical compositions comprise other excipients commonly used in injectable compositions. In some instances, the pharmaceutical compositions comprise a contrast agent to aid in visualization of the delivery of the pharmaceutical composition. In some instances, the pharmaceutical compositions comprising temsirolimus are injectable. In some instances, the pharmaceutical composition is a liquid, a suspension, a solution, or a gel.
Taxanes
In certain embodiments, the pharmaceutical compositions described herein comprise a cell division inhibitor, such as a taxane drug. In some cases, the cell division inhibitor is paclitaxel, docetaxel, cabazitaxel, or combinations thereof. The pharmaceutical compositions disclosed herein in some instances comprise paclitaxel or its pharmaceutically acceptable salts thereof. In some embodiments, the cell division inhibitor is Abraxane®.
The pharmaceutical compositions disclosed herein in some instances comprise paclitaxel or its pharmaceutically acceptable salts thereof. In some instances, the pharmaceutical compositions comprise pharmaceutically acceptable excipients. In some instances, the pharmaceutical compositions further comprise excipients including cremophor EL, ethanol, or a combination thereof. In some instances, the pharmaceutical compositions comprise nanoparticle formulations. In some instances, the pharmaceutical compositions further comprise excipients including albumin. In some instances, the pharmaceutical compositions comprise other excipients commonly used in injectable compositions. In some instances, the pharmaceutical compositions comprise a contrast agent to aid in visualization of the delivery of the pharmaceutical composition. In some instances, the pharmaceutical compositions comprising paclitaxel are injectable. In some instances, the pharmaceutical composition is a liquid, a suspension, a solution, or a gel. In some instances, pharmaceutical compositions comprise an antihistamine. In some instances, pharmaceutical compositions comprise solubilizing agents, such as polyols, glycols, or glycerols. In some instances, pharmaceutical compositions comprise macrogolglycerol ricinoleate.
In some instances, the pharmaceutical compositions disclosed herein comprise one or more glucocorticoids, such as dexamethasone, prenisolone, prednisone, triamcinoclone, hydrocortisone, methylpredinisolone, budesonide, betamethasone, deflazacort, beclomethasone, cortisone, or any other compound that targets the glucocorticoid receptor (GR). In some embodiments, the glucocorticoid is dexamethasone.
The pharmaceutical compositions disclosed herein in some instances comprise dexamethasone or its pharmaceutically acceptable salts thereof. In some instances, dexamethasone is dexamethasone sodium phosphate injection. In some instances, the pharmaceutical compositions further comprise anhydrous citric acid, sodium sulfite, benzyl alcohol, sodium citrate, water for injection, or a combination thereof. In some instances, the pharmaceutical compositions comprise pharmaceutically acceptable excipients. In some instances, the pharmaceutical compositions comprise other excipients commonly used in injectable compositions. In some instances, the pharmaceutical compositions comprise a contrast agent to aid in visualization of the delivery of the pharmaceutical composition. In some instances, the pharmaceutical composition is injectable. In some instances, the pharmaceutical composition is a liquid, a suspension, a solution, or a gel.
mTOR Inhibitors and Glucocorticoids
In certain embodiments, a cell division inhibitor, such as an mTOR inhibitor and/or a glucocorticoid (such as dexamethasone) as described herein is administered as a pure chemical. In other embodiments, the combination of mTOR inhibitor and a glucocorticoid described herein is combined with a pharmaceutically suitable or acceptable carrier (also referred to herein as a pharmaceutically suitable (or acceptable) excipient, physiologically suitable (or acceptable) excipient, or physiologically suitable (or acceptable) carrier) selected on the basis of a chosen route of administration and standard pharmaceutical practice as described, for example, in Remington: The Science and Practice of Pharmacy (Gennaro, 21st Ed. Mack Pub. Co., Easton, Pa. (2005)). In some embodiments, an mTOR inhibitor (such as temsirolimus) and a glucocorticoid (e.g., dexamethasone) are each administered as individual compositions. In some embodiments, individual compositions of an mTOR inhibitor or a glucocorticoid are combined with a suitable or acceptable excipient.
In some embodiments, mTOR inhibitor (such as temsirolimus) and a glucocorticoid (e.g., dexamethasone) are administered as a single, combined composition.
Provided herein are pharmaceutical compositions comprising an mTOR inhibitor, and a glucocorticoid together with one or more pharmaceutically acceptable carriers. The carrier(s) (or excipient(s)) is acceptable or suitable if the carrier is compatible with the other ingredients of the composition and not deleterious to the recipient (i.e., the subject or patient) of the composition.
In certain embodiments, an mTOR inhibitor and/or a glucocorticoid as described herein is substantially pure, in that it contains less than about 5%, or less than about 1%, or less than about 0.1%, of other organic small molecules, such as unreacted intermediates or synthesis by-products that are created, for example, in one or more of the steps of a synthesis method.
In some instances, the pharmaceutical compositions comprise an mTOR inhibitor and a glucocorticoid, and one or more pharmaceutically acceptable excipients. In some instances, pharmaceutical compositions comprising an mTOR inhibitor, glucocorticoid, or combination thereof comprise (by way of non-limiting example) excipients such as 0.9% sodium chloride injection USP, dehydrated alcohol, dl-alpha tocopherol, anhydrous citric acid, polysorbate 80, polyethylene glycol 400, propylene glycol, benzyl alcohol, sodium citrate, sodium sulfite, albumin, or any combination thereof. In some instances, the pharmaceutical compositions comprise nanoparticles. In some instances, the pharmaceutical compositions comprise other excipients commonly used in injectable compositions. In some instances, the pharmaceutical compositions comprise a contrast agent to aid in visualization of the delivery of the pharmaceutical composition. In some instances, the pharmaceutical compositions a liquid, a suspension, a solution, or a gel. In some instances, the pharmaceutical compositions comprising an mTOR inhibitor, a glucocorticoid, or combination thereof are injectable. In some instances, pharmaceutical compositions comprise excipients that solubilize an mTOR inhibitor, a glucocorticoid, or a combination thereof. In another embodiment, the pharmaceutical compositions comprising an mTOR inhibitor and a glucocorticoid are provided in a dosage form for parenteral administration, which comprises one or more pharmaceutically acceptable excipients or carriers. In some instances, the pharmaceutical compositions comprising an mTOR inhibitor, a glucocorticoid, or combination thereof are injectable. Where pharmaceutical compositions are formulated for intravenous, cutaneous or subcutaneous injection, the active ingredient is in the form of a parenterally acceptable aqueous solution, which is pyrogen-free and has a suitable pH, isotonicity, and stability. Those of relevant skill in the art are well able to prepare suitable solutions using, for example, isotonic vehicles, such as Sodium Chloride injection, Ringer's injection, or Lactated Ringer's injection. In some embodiments, preservatives, stabilizers, excipients, buffers, antioxidants, and/or other additives are included.
In some instances, a pharmaceutical composition comprising an mTOR inhibitor (such as temsirolimus) and a glucocorticoid (such as dexamethasone) is administered as a formulation comprising both drugs. In some instances, the percent by weight of temsirolimus to the glucocorticoid or vice versa is between 10:1 to 1:1. In some instances, the percent by weight of temsirolimus to the glucocorticoid or vice versa is between 7:1 to 2:1. In some instances, the percent by weight of temsirolimus to the glucocorticoid or vice versa is between 5:1 to 1:1. In some instances, the percent by weight of temsirolimus to the glucocorticoid or vice versa is between 3:1 to 1:1 In some instances, the percent by weight of temsirolimus to the glucocorticoid or vice versa is about 10:1, or about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, or about 1:1. In some instances, the percent by weight of temsirolimus to the glucocorticoid or vice versa is 10:1 to 1:1, 7:1 to 2:1, 5:1 to 1:1, or 3:1 to 1:1.
Taxanes and Glucocorticoids
In certain embodiments, the combination of a cell division inhibitor (such as paclitaxel) and/or a glucocorticoid (such as dexamethasone) as described herein is administered as a pure chemical. In other embodiments, the combination of cell division inhibitor and a glucocorticoid described herein is combined with a pharmaceutically suitable or acceptable carrier (also referred to herein as a pharmaceutically suitable (or acceptable) excipient, physiologically suitable (or acceptable) excipient, or physiologically suitable (or acceptable) carrier) selected on the basis of a chosen route of administration and standard pharmaceutical practice as described, for example, in Remington: The Science and Practice of Pharmacy (Gennaro, 21st Ed. Mack Pub. Co., Easton, Pa. (2005)). In some embodiments, a cell division inhibitor (such as paclitaxel) and a glucocorticoid (e.g., dexamethasone) are each administered as individual compositions. In some embodiments, individual compositions of a cell division inhibitor or a glucocorticoid are combined with a suitable or acceptable excipient.
In some embodiments, a cell division inhibitor (such as paclitaxel) and a glucocorticoid (e.g., dexamethasone) are administered as a single, combined composition.
Provided herein are pharmaceutical compositions comprising a cell division inhibitor, and a glucocorticoid together with one or more pharmaceutically acceptable carriers. The carrier(s) (or excipient(s)) is acceptable or suitable if the carrier is compatible with the other ingredients of the composition and not deleterious to the recipient (i.e., the subject or patient) of the composition.
In certain embodiments, a cell division inhibitor and/or a glucocorticoid as described herein is substantially pure, in that it contains less than about 5%, or less than about 1%, or less than about 0.1%, of other organic small molecules, such as unreacted intermediates or synthesis by-products that are created, for example, in one or more of the steps of a synthesis method.
In some instances, the pharmaceutical compositions comprise a cell division inhibitor and a glucocorticoid, and one or more pharmaceutically acceptable excipients. In some instances, pharmaceutical compositions comprising a cell division inhibitor, glucocorticoid, or combination thereof comprise (by way of non-limiting example) excipients such as 0.9% sodium chloride injection USP, dehydrated alcohol, dl-alpha tocopherol, anhydrous citric acid, polysorbate 80, polyethylene glycol 400, propylene glycol, benzyl alcohol, sodium citrate, sodium sulfite, cremophor EL, albumin, or any combination thereof. In some instances, the pharmaceutical compositions comprise nanoparticles. In some instances, the pharmaceutical compositions comprise other excipients commonly used in injectable compositions. In some instances, the pharmaceutical compositions comprise a contrast agent to aid in visualization of the delivery of the pharmaceutical composition. In some instances, the pharmaceutical compositions comprise a liquid, a suspension, a solution, or a gel. In some instances, the pharmaceutical compositions comprising a cell division inhibitor, a glucocorticoid, or combination thereof are injectable. In some instances, pharmaceutical compositions comprise excipients that solubilize a cell division inhibitor, a glucocorticoid, or a combination thereof. In another embodiment, the pharmaceutical compositions comprising a cell division inhibitor and a glucocorticoid are provided in a dosage form for parenteral administration, which comprises one or more pharmaceutically acceptable excipients or carriers. In some instances, the pharmaceutical compositions comprising a cell division inhibitor, a glucocorticoid, or combination thereof are injectable. Where pharmaceutical compositions are formulated for intravenous, cutaneous or subcutaneous injection, the active ingredient is in the form of a parenterally acceptable aqueous solution, which is pyrogen-free and has a suitable pH, isotonicity, and stability. Those of relevant skill in the art are well able to prepare suitable solutions using, for example, isotonic vehicles, such as Sodium Chloride injection, Ringer's injection, or Lactated Ringer's injection. In some embodiments, preservatives, stabilizers, excipients, buffers, antioxidants, and/or other additives are included.
In some instances, a pharmaceutical composition comprising a cell division inhibitor (such as paclitaxel) and a glucocorticoid (such as dexamethasone) is administered as a formulation comprising both drugs. In some instances, the percent by weight of paclitaxel to the glucocorticoid or vice versa is between 10:1 to 1:1. In some instances, the percent by weight of paclitaxel to the glucocorticoid or vice versa is between 7:1 to 2:1. In some instances, the percent by weight of paclitaxel to the glucocorticoid or vice versa is between 5:1 to 1:1. In some instances, the percent by weight of paclitaxel to the glucocorticoid or vice versa is between 3:1 to 1:1 In some instances, the percent by weight of paclitaxel to the glucocorticoid or vice versa is about 10:1, or about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, or about 1:1. In some instances, the percent by weight of paclitaxel to the glucocorticoid or vice versa is 10:1 to 1:1, 7:1 to 2:1, 5:1 to 1:1, or 3:1 to 1:1.
In atherosclerosis, bypass graft failure, transplant vasculopathy, and in-stent restenosis, the activation and proliferation of vascular smooth muscle cells (VSMCs) located in the vessel wall are linked to inflammation, apoptosis, and matrix alterations observed in these pathophysiologic diseases. An established strategy for the treatment of these conditions is to inhibit cellular proliferation through the use of paclitaxel. Paclitaxel has cytotoxic effects and may not provide the most durable solution for vascular pathologies. Some embodiments herein describe the use of mTOR inhibitors combined with glucocorticoids to provide a safer and more superior approach to the regulation of activated VSMCs compared to anti-proliferatives such as paclitaxel. Other embodiments herein describe the use of cell division inhibitors such as paclitaxel combined with glucocorticoids to provide safer and more superior approaches to the regulation of activated VSMCs compared to anti-proliferatives such as paclitaxel used alone.
Mechanistic target of rapamycin (mTOR) inhibitors have been identified drugs for treating vascular disease. A member of phosphatidylinositol-3 kinase-related kinase (PI3K) family, mTOR is involved in regulating cell growth, proliferation, cell survival and angiogenesis. In response to physical insult of revascularization procedure, smooth muscle and endothelial cells in blood vessels activate stress response pathways, which lead to cell proliferation, secretion of pro-inflammatory mediators and extracellular matrix components, and ultimately to restenosis. In some instances, drugs successful in blocking one or more of the stress response pathways decrease the degree of restenosis. In certain instances, mTOR inhibitors reduce cellular proliferation and inflammation and have been used successfully in graft-versus-host disease, in organ transplant and in some cancers by blocking mTOR activation in response to insulin, growth factors and amino acids.
mTOR inhibitors include the original mTOR inhibitor, sirolimus, also known as rapamycin, and the analogs of sirolimus. These analogs include everolimus, zotarolimus, deforolimus, biolimus and temsirolimus.
Temsirolimus is approved for the treatment of renal cell carcinoma (RCC), but it is not approved for the treatment of vascular restenosis. Temsirolimus has been described as a prodrug for sirolimus (i.e., temsirolimus is metabolized to the active agent sirolimus). As there are fewer metabolic reactions in the vascular tissue compared to the liver (where systemic drugs are generally metabolized), one would not expect temsirolimus to be efficacious when administered directly to vascular and other luminal walls. Some embodiments provided herein describe temsirolimus as an active agent useful for inhibiting mTOR and disrupting the mitosis of cells. In some embodiments, temsirolimus is useful for the treatment of vascular disease. In further or additional embodiments, temsirolimus is useful for the treatment of vascular disease via direct injection into vascular or other luminal walls. In further or additional embodiments, temsirolimus in combination with a glucocorticoid (e.g., dexamethasone) is useful for the treatment of vascular disease via direct injection into vascular or other luminal walls.
Also described herein are methods, devices, and compositions wherein in some embodiments a synergistic effect is observed for the combination of -limus analogs (such as temsirolimus) with corticosteroids (such as dexamethasone) for their ability to reduce the signals causing hyperplasia and sclerosis and to further reduce the cellular activities which apparently cause hyperplasia and sclerosis. Further described herein are methods wherein, in an attempt to influence the healing process, the combination of dexamethasone and temsirolimus, exemplary agents chosen from the category of corticosteroids (such as glucocorticoids) and mTOR inhibitors, respectively, target the disease process. In other methods described herein, the combination of dexamethasone and paclitaxel, exemplary agents chosen from the category of corticosteroids (such as glucocorticoids) and cell division inhibitors, respectively, target the disease process. In other methods described herein, the combination of dexamethasone and paclitaxel, exemplary agents chosen from the category of corticosteroids (such as glucocorticoids) and cell division inhibitors, respectively, influences the healing process. In some embodiments, the deployment or delivery of the drug takes place from the luminal side via drug-coated balloons or pressurized catheters, or from within the vessel wall (the media, the adventitia, or the perivascular tissues) with the use of needle injection catheters that deploy a needle from the inside to the outside of the artery. In some embodiments, drug delivery is accomplished using ultrasound guidance and penetration of a needle through the skin and surrounding tissues to the perivascular area in order to bathe the target tissues in the combination drug.
Subjects treated by the methods disclosed herein can exhibit a vascular disease. In one example, the vascular disease is atherosclerosis in the heart, legs, carotid, or renal blood vessels. In another example, the vascular disease is peripheral artery disease (PAD). In another example, the vascular disease is angina, myocardial infarction, congestive heart failure, cardiac arrhythmia, peripheral artery disease, claudication, or critical limb ischemia. In another example, the vascular disease is atherosclerosis, bypass graft failure, transplant vasculopathy, in-stent restenosis, or restenosis. In one example, the vascular disease is blood clots, thrombus, or other blockages in a blood vessel that may occlude peripheral blood flow, leading to tissue and organ necrosis. In one example, the vascular disease is PAD in renal artery or carotid artery. In some examples, the subject with restenosis had a procedure to improve the patency of the blood vessel or a revascularization procedure previously. In some instances, the vascular disease is restenosis.
In some cases, the disease site of the vascular disease includes blood vessels and the tissues surrounding the blood vessel. The vasculature of a subject refers to the circulatory system and in some instances comprises the arterial system, venous systems, or both arterial and venous systems and the blood vessels within those systems. In some examples, the blood vessel is an artery, arteriole, or other blood vessels of the arterial system. In some examples, the blood vessel is a vein, venule, or other blood vessels of the venous system. In one example, the artery is a coronary artery or a peripheral artery. In one example, the artery is below the knee. In another example, the artery is in the leg above the knee. In another example, the blood vessel is below-knee popliteal vessel or tibial vessel. In some examples, the blood vessel is a femoral vessel. In some examples, the artery is renal artery, carotid artery, cerebral artery, pulmonary artery, or artery in the leg. In some examples, the artery is a femoral artery.
Restenosis is in various tissues and blood vessels in the body. In some instances, the restenosis is in a coronary vessel. In some instances, the restenosis is in a coronary artery. In some instances, the restenosis is in a peripheral artery. In some instances, the restenosis is in the leg. In other instances, the restenosis is below the knee or in the leg above the knee. In some instances, the restenosis is in a below-knee popliteal vessel or tibial vessel. In some instances, the restenosis is in a femoral vessel. In other instances, the restenosis is in a femoral artery.
In some instances, the tissue surrounding a blood vessel refers to any tissues outside the endothelial cell wall of the blood vessel that is radially away from the lumen of the blood vessel in a cross section and includes plaque and calcification. In some instances, the tissue surrounding a blood vessel comprises adventitial tissue, perivascular tissue, or any tissue surrounding the endothelial wall of a blood vessel. In some instances, adventitial tissue is also known as adventitia or tunica adventitia or tunica externa. In some instances, adventitial tissue is outside of the external elastic membrane. In some instances, the tissue surrounding a blood vessel is tissues outside the tunica intima of the blood vessel. In some instances, the tissue surrounding a blood vessel is tissues outside the tunica media of the blood vessel. In some instances, the tissue surrounding a blood vessel is tissues outside the internal elastic membrane. In some instances, the tissue is a connective tissue. In some instances, the tissue is diseased tissue such as plaque, fibrosis, calcification, or combinations of diseased and healthy tissues.
In some instances, “patency” refers to blood vessel openness. In some instances, patency at the disease site refers to patency of the blood vessel, or blood vessel openness, at the disease site. In some instances, vessel cross-sectional area at the disease site refers to patency of the blood vessel at the disease site. In some instances, vessel cross-sectional area is determined by angiography. In some instances, the angiography is quantitative vascular angiography (QVA). In other instances, vessel cross-sectional area is determined by intravascular ultrasound (IVUS). In some instances, patency is described as percent of diameter of the lumen of the blood vessel that is open and unobstructed. In some instances, patency is described as percent of cross sectional area of the lumen of the blood vessel, or vessel cross-sectional area, that is open and unobstructed. In other instances, patency is the percent of luminal volume that is open and unobstructed. In some instances, patency requires determination of the boundaries of the endothelial wall of the blood vessel. In some instances, a blood vessel that is completely open and unobstructed has 100% patency; i.e., the blood vessel has a cross-sectional area that is healthy and typical of a normal, healthy blood vessel in the same part of the body. In some instances, a blood vessel that is completely blocked and obstructed has 0% patency. In some instances, patency is the binary measure of openness greater than 50% in diameter compared to adjacent non-diseased vessel. In some instances, patency is the binary measure of openness greater than 50% in cross-sectional area compared to adjacent non-diseased vessel. In some instances, patency is the binary measure of openness greater than 50% in luminal volume compared to adjacent non-diseased vessel.
In some instances, a “therapeutically effective amount” refers to an amount of drug (e.g., temsirolimus or dexamethasone) that increases vessel cross-sectional area at the disease site. In some instances, therapeutically effective refers to increasing the vessel cross-sectional area at the disease site after administration of a pharmaceutical composition. In some instances, therapeutically effective refers to minimally decreasing the vessel cross-sectional area at the disease site after administration when compared to the vessel cross-sectional area at the disease site at the time of administration. In some instances, therapeutically effective refers to increasing the vessel cross-sectional area at the disease site. In some instances, therapeutically effective refers to increasing minimally the vessel cross-sectional area at the disease site after administration when compared to the vessel cross-sectional area at the disease site at the time of administration. In some instances, therapeutically effective refers to decreasing the vessel cross-sectional area at the disease site no more than 30%, 20%, 10%, or 0% when compared to the vessel cross-sectional area at the disease site at the time of administration; in other words the patency decreases no more than 30%, 20%, 10%, or 0% when compared to the patency at the disease site at the time of administration. In some instances, the vessel cross-sectional area at the disease site decreases no more than 60%, 50%, 40%, 30%, 20%, or 10% when compared to vessel cross-sectional area at the disease site at the time of administration. In some instances, the vessel cross-sectional area at the disease site increases at least 60%, 50%, 40%, 30%, 20%, or 10% when compared to vessel cross-sectional area at the disease site at the time of administration.
In some instances, the pharmaceutical composition is injected at various locations at or near the disease site. In some instances, the disease site refers to a blood vessel affected by a vascular disease. In some instances, the disease site refers to a blood vessel with a partial or complete blockage of the lumen. In some instances, the disease site refers to a blood vessel with a vessel cross-sectional area of less than 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, or 0% of vessel cross-sectional area of an unobstructed vessel as determined from the vessel wall. In some instances, the pharmaceutical composition is injected distal or proximal to the disease site. In some instances, the pharmaceutical composition is injected at least about 2 cm away from the disease site. In some instances, the pharmaceutical composition is injected at or adjacent to the disease site. In some instances, the pharmaceutical composition is injected into a blood vessel. In some instances, the pharmaceutical composition is injected into an adventitial tissue surrounding a blood vessel. In some instances, the pharmaceutical composition is injected into a perivascular tissue surrounding a blood vessel.
Temsirolimus administered as part of a combination therapy with a glucocorticoid in some instances has a range of doses that are therapeutically effective for treating the vascular disease. In some instances, the therapeutically effective amount of temsirolimus is about 1 μg to 50 mg, about 10 μg to 20 mg, about 25 μg to 10 mg, about 1 μg to 2 mg, about 10 μg to 500 μg, about 100 μg to about 2 mg or about 100 μg to 500 μg. In some instances, the therapeutically effective amount of temsirolimus is about 100 μg to 5 mg. In some instances, the therapeutically effective amount of temsirolimus is about 100 μg to 15 mg. In some instances, the therapeutically effective amount of temsirolimus is about 10 μg, about 25 μg, about 50 μg, about 100 μg, about 500 μg, about 1.0 mg, about 5.0 mg, about 10.0 mg, or about 15.0 mg. In some instances, the therapeutically effective volume of temsirolimus is about 0.01 ml to about 50 ml, about 0.5 ml to about 20 ml, about 0.5 ml to about 25 ml, about 0.5 ml to about 5 ml, or about 1 ml to about 5 ml. In some instances, the therapeutically effective concentration of temsirolimus is about 0.1 mg/mL to about 0.4 mg/mL, about 0.1 mg/mL to about 0.5 mg/mL, or about 0.01 mg/mL to about 2.0 mg/mL. In some instances, the therapeutically effective concentration of temsirolimus is about 0.1 mg/mL, about 0.2 mg/mL, about 0.3 mg/mL, about 0.4 mg/mL, about 0.5 mg/mL, about 1.0 mg/ml, about 1.5 mg/ml, about 2.0 mg/ml, about 2.5 mg/ml, or 3.0 mg/ml. In some instances, the therapeutically effective amount of temsirolimus is about 0.005 mg to 5 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.025 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.05 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel, or about 0.1 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel. In some instances, the therapeutically effective amount of temsirolimus is about 0.025 mg to 1 mg per cm of longitudinal length of the blood vessel. The longitudinal length of the disease site in the blood vessel, also known as the longitudinal length of the lesion, is about 1 cm, 5 cm, 10 cm, 20 cm, 30 cm, 40 cm, or 50 cm.
Paclitaxel administered as part of a combination therapy with a glucocorticoid in some instances has a range of doses that are therapeutically effective for treating the vascular disease. In some instances, the therapeutically effective amount of paclitaxel is about 1 μg to 50 mg, about 10 μg to 20 mg, about 25 μg to 10 mg, about 1 μg to 2 mg, about 10 μg to 500 μg, about 100 μg to about 2 mg or about 100 μg to 500 μg. In some instances, the therapeutically effective amount of paclitaxel is about 10 μg, about 25 μg, about 50 μg, about 100 μg, about 500 μg, about 1.0 mg, about 5.0 mg, about 10.0 mg, or about 15.0 mg. In some instances, the therapeutically effective volume of paclitaxel is about 0.01 ml to about 50 ml, about 0.5 ml to about 20 ml, about 0.5 ml to about 25 ml, about 0.5 ml to about 5 ml, or about 1 ml to about 5 ml. In some instances, the therapeutically effective concentration of paclitaxel is about 0.1 mg/mL to about 0.4 mg/mL, about 0.1 mg/mL to about 0.5 mg/mL, or about 0.01 mg/mL to about 2.0 mg/mL. In some instances, the therapeutically effective concentration of paclitaxel is about 0.1 mg/mL, about 0.2 mg/mL, about 0.3 mg/mL, about 0.4 mg/mL, about 0.5 mg/mL, about 1.0 mg/ml, about 1.5 mg/ml, about 2.0 mg/ml, about 2.5 mg/ml, or 3.0 mg/ml. In some instances, the therapeutically effective amount of paclitaxel is about 0.005 mg to 5 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.025 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.05 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel, or about 0.1 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel. In some instances, the therapeutically effective amount of paclitaxel is about 0.025 mg to 1 mg per cm of longitudinal length of the blood vessel. The longitudinal length of the disease site in the blood vessel, also known as the longitudinal length of the lesion, is about 1 cm, 5 cm, 10 cm, 20 cm, 30 cm, 40 cm, or 50 cm.
A glucocorticoid administered in combination with temsirolimus comprises a range of dosages that are therapeutically effective for treating the vascular disease. In some instances, the glucocorticoid is dexamethasone. In some instances, the therapeutically effective amount of dexamethasone is about 1 μg to 50 mg, about 10 μg to 20 mg, about 25 μg to 10 mg, about 1 μg to 2 mg, about 10 μg to 500 μg, about 100 μg to 50 mg, about 100 μg to 20 mg, about 100 μg to 10 mg, about 100 μg to 1 mg, or about 100 μg to 500 μg. In some instances, the therapeutically effective amount of dexamethasone is about 10 μg, about 25 μg, about 50 μg, about 100 μg, about 500 μg, about 1.0 mg, about 5.0 mg, about 10.0 mg, about 20.0 mg, about 30.0 mg, about 40.0 mg, or about 50.0 mg. In some instances, the therapeutically effective volume of dexamethasone is about 0.01 mL to about 50 mL, about 0.5 mL to about 20 mL, about 0.5 mL to about 25 mL, about 0.5 mL to about 5 mL, 1 mL to 10 mL, or about 1 mL to about 5 mL. In some instances, the therapeutically effective concentration of dexamethasone is about 0.1 mg/mL to about 0.4 mg/mL, about 0.1 mg/mL to about 0.5 mg/mL, about 0.01 mg/mL to about 2.0 mg/mL, or about 1.0 mg/mL to about 10.0 mg/mL. In some instances, the therapeutically effective concentration of dexamethasone is about 0.1 mg/mL, about 0.2 mg/mL, about 0.3 mg/mL, about 0.4 mg/mL, about 0.5 mg/mL, about 1.0 mg/ml, about 1.5 mg/ml, about 2.0 mg/ml, about 2.5 mg/ml, about 3.0 mg/ml, about 4.0 mg/mL, about 6.0 mg/mL, about 8.0 mg/mL or about 10.0 mg/mL. In some instances, the therapeutically effective amount of dexamethasone is about 0.05 mg to 10 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.05 mg to 7 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.1 mg to 4 mg per cm of longitudinal length of the disease site in the blood vessel, or about 0.1 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel. In some instances, the therapeutically effective amount of dexamethasone is about 0.8 mg to 8 mg per cm of longitudinal length of the disease site in the blood vessel. In some instances, the therapeutically effective amount of dexamethasone is about 0.1 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel. The longitudinal length of the disease site in the blood vessel, also known as the longitudinal length of the lesion, is about 1 cm, 5 cm, 10 cm, 20 cm, 30 cm, 40 cm, or 50 cm.
A glucocorticoid administered in combination with paclitaxel comprises a range of dosages that are therapeutically effective for treating the vascular disease. In some instances, the glucocorticoid is dexamethasone. In some instances, the therapeutically effective amount of dexamethasone is about 1 μg to 50 mg, about 10 μg to 20 mg, about 25 μg to 10 mg, about 1 μg to 2 mg, about 10 μg to 500 μg, about 100 μg to 50 mg, about 100 μg to 20 mg, about 100 μg to 10 mg, about 100 μg to 1 mg, or about 100 μg to 500 μg. In some instances, the therapeutically effective amount of dexamethasone is about 10 μg, about 25 μg, about 50 μg, about 100 μg, about 500 μg, about 1.0 mg, about 5.0 mg, about 10.0 mg, about 20.0 mg, about 30.0 mg, about 40.0 mg, or about 50.0 mg. In some instances, the therapeutically effective volume of dexamethasone is about 0.01 mL to about 50 mL, about 0.5 mL to about 20 mL, about 0.5 mL to about 25 mL, about 0.5 mL to about 5 mL, 1 mL to 10 mL, or about 1 mL to about 5 mL. In some instances, the therapeutically effective concentration of dexamethasone is about 0.1 mg/mL to about 0.4 mg/mL, about 0.1 mg/mL to about 0.5 mg/mL, about 0.01 mg/mL to about 2.0 mg/mL, or about 1.0 mg/mL to about 10.0 mg/mL. In some instances, the therapeutically effective concentration of dexamethasone is about 0.1 mg/mL, about 0.2 mg/mL, about 0.3 mg/mL, about 0.4 mg/mL, about 0.5 mg/mL, about 1.0 mg/ml, about 1.5 mg/ml, about 2.0 mg/ml, about 2.5 mg/ml, about 3.0 mg/ml, about 4.0 mg/mL, about 6.0 mg/mL, about 8.0 mg/mL or about 10.0 mg/mL. In some instances, the therapeutically effective amount of dexamethasone is about 0.05 mg to 10 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.05 mg to 7 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.1 mg to 4 mg per cm of longitudinal length of the disease site in the blood vessel, or about 0.1 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel. In some instances, the therapeutically effective amount of dexamethasone is about 0.8 mg to 8 mg per cm of longitudinal length of the disease site in the blood vessel. In some instances, the therapeutically effective amount of dexamethasone is about 0.1 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel. The longitudinal length of the disease site in the blood vessel, also known as the longitudinal length of the lesion, is about 1 cm, 5 cm, 10 cm, 20 cm, 30 cm, 40 cm, or 50 cm.
A pharmaceutical composition of temsirolimus administered in combination with a glucocorticoid comprises a range of dosages that are therapeutically effective for treating the vascular disease. In some instances, the therapeutically effective amount of temsirolimus is about 1 μg to 50 mg, about 10 μg to 20 mg, about 25 μg to 10 mg, about 1 μg to 2 mg, about 10 μg to 500 μg, about 100 μg to about 2 mg or about 100 μg to 500 μg. In some instances, the therapeutically effective amount of temsirolimus is about 10 μg, about 25 μg, about 50 μg, about 100 μg, about 500 μg, about 1.0 mg, about 5.0 mg, about 10.0 mg, or about 15.0 mg. In some instances, the therapeutically effective volume of temsirolimus is about 0.01 ml to about 50 ml, about 0.5 ml to about 20 ml, about 0.5 ml to about 25 ml, about 0.5 ml to about 5 ml, or about 1 ml to about 5 ml. In some instances, the therapeutically effective concentration of temsirolimus is about 0.1 mg/mL to about 0.4 mg/mL, about 0.1 mg/mL to about 0.5 mg/mL, or about 0.01 mg/mL to about 2.0 mg/mL. In some instances, the therapeutically effective concentration of temsirolimus is about 0.1 mg/mL, about 0.2 mg/mL, about 0.3 mg/mL, about 0.4 mg/mL, about 0.5 mg/mL, about 1.0 mg/ml, about 1.5 mg/ml, about 2.0 mg/ml, about 2.5 mg/ml, or 3.0 mg/ml. In some instances, the therapeutically effective amount of temsirolimus is about 0.005 mg to 5 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.025 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.05 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel, or about 0.1 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel. In some instances, the therapeutically effective amount of temsirolimus is about 0.025 mg to 1 mg per cm of longitudinal length of the blood vessel. The longitudinal length of the disease site in the blood vessel, also known as the longitudinal length of the lesion, is about 1 cm, 5 cm, 10 cm, 20 cm, 30 cm, 40 cm, or 50 cm.
Alternatively, a pharmaceutical composition of paclitaxel administered in combination with a glucocorticoid comprises a range of dosages that are therapeutically effective for treating the vascular disease. In some instances, the therapeutically effective amount of paclitaxel is about 1 μg to 50 mg, about 10 μg to 20 mg, about 25 μg to 10 mg, about 1 μg to 2 mg, about 10 μg to 500 μg, about 100 μg to about 2 mg or about 100 μg to 500 μg. In some instances, the therapeutically effective amount of paclitaxel is about 10 μg, about 25 μg, about 50 μg, about 100 μg, about 500 μg, about 1.0 mg, about 5.0 mg, about 10.0 mg, or about 15.0 mg. In some instances, the therapeutically effective volume of paclitaxel is about 0.01 ml to about 50 ml, about 0.5 ml to about 20 ml, about 0.5 ml to about 25 ml, about 0.5 ml to about 5 ml, or about 1 ml to about 5 ml. In some instances, the therapeutically effective concentration of paclitaxel is about 0.1 mg/mL to about 0.4 mg/mL, about 0.1 mg/mL to about 0.5 mg/mL, or about 0.01 mg/mL to about 2.0 mg/mL. In some instances, the therapeutically effective concentration of paclitaxel is about 0.1 mg/mL, about 0.2 mg/mL, about 0.3 mg/mL, about 0.4 mg/mL, about 0.5 mg/mL, about 1.0 mg/ml, about 1.5 mg/ml, about 2.0 mg/ml, about 2.5 mg/ml, or 3.0 mg/ml. In some instances, the therapeutically effective amount of paclitaxel is about 0.005 mg to 5 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.025 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.05 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel, or about 0.1 mg to 1 mg per cm of longitudinal length of the disease site in the blood vessel. In some instances, the therapeutically effective amount of paclitaxel is about 0.025 mg to 1 mg per cm of longitudinal length of the blood vessel. The longitudinal length of the disease site in the blood vessel, also known as the longitudinal length of the lesion, is about 1 cm, 5 cm, 10 cm, 20 cm, 30 cm, 40 cm, or 50 cm.
A pharmaceutical composition of a glucocorticoid administered in combination with temsirolimus comprises a range of dosages that are therapeutically effective for treating the vascular disease. In some instances, the glucocorticoid is dexamethasone. In some instances, the therapeutically effective amount of dexamethasone is about 1 μg to 50 mg, about 10 μg to 20 mg, about 25 μg to 10 mg, about 1 μg to 2 mg, about 10 μg to 500 μg, about 100 μg to 50 mg, about 100 μg to 20 mg, about 100 μg to 10 mg, about 100 μg to 1 mg, or about 100 μg to 500 μg. In some instances, the therapeutically effective amount of dexamethasone is about 10 μg, about 25 μg, about 50 μg, about 100 μg, about 500 μg, about 1.0 mg, about 5.0 mg, about 10.0 mg, about 20.0 mg, about 30.0 mg, about 40.0 mg, or about 50.0 mg. In some instances, the therapeutically effective volume of dexamethasone is about 0.01 mL to about 50 mL, about 0.5 mL to about 20 mL, about 0.5 mL to about 25 mL, about 0.5 mL to about 5 mL, 1 mL to 10 mL, or about 1 mL to about 5 mL. In some instances, the therapeutically effective concentration of dexamethasone is about 0.1 mg/mL to about 0.4 mg/mL, about 0.1 mg/mL to about 0.5 mg/mL, about 0.01 mg/mL to about 2.0 mg/mL, or about 1.0 mg/mL to about 10.0 mg/mL. In some instances, the therapeutically effective concentration of dexamethasone is about 0.1 mg/mL, about 0.2 mg/mL, about 0.3 mg/mL, about 0.4 mg/mL, about 0.5 mg/mL, about 1.0 mg/ml, about 1.5 mg/ml, about 2.0 mg/ml, about 2.5 mg/ml, about 3.0 mg/ml, about 4.0 mg/mL, about 6.0 mg/mL, about 8.0 mg/mL or about 10.0 mg/mL. In some instances, the therapeutically effective amount of dexamethasone is about 0.05 mg to 10 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.05 mg to 7 mg per cm of longitudinal length of the disease site in the blood vessel, about 0.1 mg to 4 mg per cm of longitudinal length of the disease site in the blood vessel, or about 0.1 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel. In some instances, the therapeutically effective amount of dexamethasone is about 0.1 mg to 2 mg per cm of longitudinal length of the disease site in the blood vessel. The longitudinal length of the disease site in the blood vessel, also known as the longitudinal length of the lesion, is about 1 cm, 5 cm, 10 cm, 20 cm, 30 cm, 40 cm, or 50 cm.
The dose of the composition comprising temsirolimus and a glucocorticoid as described herein differ, depending upon the patient's condition, that is, stage of the disease, general health status, age, and other factors.
The dose of the composition comprising paclitaxel and a glucocorticoid as described herein differ, depending upon the patient's condition, that is, stage of the disease, general health status, age, and other factors.
Pharmaceutical compositions described herein are administered in a manner appropriate to the disease to be treated (or prevented). An appropriate dose and a suitable duration and frequency of administration will be determined by such factors as the condition of the patient, the type and severity of the patient's disease, the particular form of the active ingredient, and the method of administration. In general, an appropriate dose and treatment regimen provides the composition(s) in an amount sufficient to provide therapeutic and/or prophylactic benefit (e.g., an improved clinical outcome), or a lessening of symptom severity. Optimal doses are generally determined using experimental models and/or clinical trials. The optimal dose depends upon the body mass, weight, or blood volume of the patient.
In one embodiment, the pharmaceutical compositions of temsirolimus and a glucocorticoid described herein, or pharmaceutically acceptable salts thereof, are used in the preparation of medicaments for the treatment of diseases or conditions in a mammal that would benefit from administration of any one of the compounds disclosed. In one embodiment, the pharmaceutical compositions of paclitaxel and a glucocorticoid described herein, or pharmaceutically acceptable salts thereof, are used in the preparation of medicaments for the treatment of diseases or conditions in a mammal that would benefit from administration of any one of the compounds disclosed. Methods for treating any of the diseases or conditions described herein in a mammal in need of such treatment, involves administration of pharmaceutical compositions that include at least one compound described herein or a pharmaceutically acceptable salt, active metabolite, prodrug, or pharmaceutically acceptable solvate thereof, in therapeutically effective amounts to said mammal.
In certain embodiments, the pharmaceutical compositions of temsirolimus and a glucocorticoid described herein are administered for prophylactic and/or therapeutic treatments. In certain embodiments, the pharmaceutical compositions of paclitaxel and a glucocorticoid described herein are administered for prophylactic and/or therapeutic treatments. In certain therapeutic applications, the compositions are administered to a patient already suffering from a disease or condition, in an amount sufficient to cure or at least partially arrest at least one of the symptoms of the disease or condition. Amounts effective for this use depend on the severity and course of the disease or condition, previous therapy, the patient's health status, weight, and response to the drugs, and the judgment of the treating physician. Therapeutically effective amounts are optionally determined by methods including, but not limited to, a dose escalation and/or dose ranging clinical trial.
In prophylactic applications, the pharmaceutical composition of temsirolimus and a glucocorticoid described herein are administered to a patient susceptible to or otherwise at risk of a particular disease, disorder or condition. In prophylactic applications, the pharmaceutical composition of paclitaxel and a glucocorticoid described herein are administered to a patient susceptible to or otherwise at risk of a particular disease, disorder or condition. Such an amount is defined to be a “prophylactically effective amount or dose.” In this use, the precise amounts also depend on the patient's state of health, weight, and the like. When used in patients, effective amounts for this use will depend on the severity and course of the disease, disorder or condition, previous therapy, the patient's health status and response to the drugs, and the judgment of the treating physician. In one aspect, prophylactic treatments include administering to a mammal, in which the mammal previously experienced at least one symptom of the disease being treated and is currently in remission, a pharmaceutical composition comprising a compound described herein, or a pharmaceutically acceptable salt thereof, in order to prevent a return of the symptoms of the disease or condition.
In certain embodiments wherein the patient's condition does not improve, upon the doctor's discretion the administration of a pharmaceutical composition of temsirolimus and a glucocorticoid are administered chronically, that is, for an extended period of time, including throughout the duration of the patient's life in order to ameliorate or otherwise control or limit the symptoms of the patient's disease or condition. In certain embodiments wherein the patient's condition does not improve, upon the doctor's discretion the administration of a pharmaceutical composition of paclitaxel and a glucocorticoid are administered chronically, that is, for an extended period of time, including throughout the duration of the patient's life in order to ameliorate or otherwise control or limit the symptoms of the patient's disease or condition.
Once improvement of the patient's conditions has occurred, a maintenance injection is administered if necessary. Subsequently, in specific embodiments, the dosage or the frequency of administration, or both, is reduced, as a function of the symptoms, to a level at which the improved disease, disorder or condition is retained. In certain embodiments, however, the patient requires intermittent treatment on a long-term basis upon any recurrence of symptoms.
Toxicity and therapeutic efficacy of such therapeutic regimens are determined by standard pharmaceutical procedures in cell cultures or experimental animals, including, but not limited to, the determination of the LD50 and the ED50. The dose ratio between the toxic and therapeutic effects is the therapeutic index and it is expressed as the ratio between LD50 and ED50. In certain embodiments, the data obtained from cell culture assays and animal studies are used in formulating the therapeutically effective daily dosage range and/or the therapeutically effective unit dosage amount for use in mammals, including humans. In some embodiments, the dosage amount of the compounds described herein lies within a range of circulating concentrations that include the ED50 with minimal toxicity. In certain embodiments, the dosage range and/or the unit dosage amount varies within this range depending upon the dosage form employed and the route of administration utilized.
In any of the aforementioned aspects are further embodiments comprising single administrations of the effective amount of the pharmaceutical composition of temsirolimus and a glucocorticoid described herein, including further embodiments in which the composition is administered once a month, twice a month, 3 times a month, once every 2 months, once every 3 months, once every 4 months, once every 5 months, or once every 6 months.
In any of the aforementioned aspects are further embodiments comprising single administrations of the effective amount of the pharmaceutical composition of paclitaxel and a glucocorticoid described herein, including further embodiments in which the composition is administered once a month, twice a month, 3 times a month, once every 2 months, once every 3 months, once every 4 months, once every 5 months, or once every 6 months.
In certain embodiments wherein a patient's status does improve, the dose of drug being administered is temporarily reduced or temporarily suspended for a certain length of time (e.g., a “drug holiday”). In specific embodiments, the length of the drug holiday is between 1 month and 5 years, including by way of example only, 2 months, 3 months, 4 months, 5 months, 6 months, 7 months, 1 year, 2 years, 3 years, 4 years, 5 years, or more than 5 years. The dose reduction during a drug holiday is, by way of example only, by 10%-100%, including by way of example only 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, and 100%. In further or alternative embodiments, the method comprises a drug holiday, wherein the administration of the compound is temporarily suspended or the dose of the compound being administered is temporarily reduced; at the end of the drug holiday, dosing of the compound is resumed. In one embodiment, the length of the drug holiday varies from 6 months to 2 years. In one embodiment, the length of the drug holiday is 1 year. In one embodiment, the length of the drug holiday is 2 years. In one embodiment, the length of the drug holiday is 3 years.
Described herein are devices and methods for administration of pharmaceutical compositions. As an alternative to stent-based luminal drug delivery, the direct delivery of drug into vascular and other luminal walls in some instances enhances the therapeutic concentrations of pharmaceutical agents in targeted tissues. For example, it is in some cases particularly desirable to provide for an extended volumetric distribution of the delivered pharmaceutical agent including both longitudinal and radial spreading from the injection site(s) in order to provide therapeutic dosage levels of the agent within the targeted tissue region. In some instances, devices efficiently deliver the drugs into the targeted tissue and limit or avoid the loss of drugs into the luminal blood flow. In some instances, the persistence of such therapeutic concentrations of the pharmaceutical agent in the tissue were also increased, particularly in targeted tissues away from the blood vessel wall, including the adventitial tissue surrounding the blood vessel wall. In some instances, the uniformity and extent of pharmaceutical agent delivery over remote, extended, and distributed regions of the adventitia and other tissues surrounding the blood vessels is increased. In some instances, pharmaceutical agents are delivered through the blood vessel walls at non-diseased sites within the blood vessel, where the agent then migrate through the adventitia or other tissues to the diseased site(s). In some instances, intravascular delivery of pharmaceutical agents treats diseases and conditions of the tissues and organs in addition to those directly related to the vasculature.
In some cases, drug injection or infusion catheters and devices are suitable for use with the methods described herein to inject pharmaceutical compositions into blood vessels the treat restenosis. An example of a device includes the Mercator Bullfrog® Micro-Infusion Device available from Mercator MedSystems of Emeryville, Calif. Other examples include the devices described in U.S. patent applications Ser. Nos. 14/605,865 and 15/691,138, the entire disclosures of which are incorporated herein by reference. Examples of suitable devices and their use are described as follows. In some instances, injections are performed using needles or microneedles.
In some cases, a pharmaceutical composition to treat the vascular disease is delivered to the tissue surrounding a blood vessel using a drug injection or infusion catheter. In one example of a drug injection or infusion catheter as shown in
Retaining rings 22a and 22b are located at the distal and proximal ends, respectively, of the actuator. The catheter tip is joined to the retaining ring 22a, while the catheter lead is joined to retaining ring 22b. The retaining rings are made of a thin, on the order of 10 to 100 microns (μm), substantially flexible but relatively non-distensible material, such as Parylene (types C, D or N), or a metal, for example, aluminum, stainless steel, gold, titanium or tungsten. The retaining rings form a flexible but relatively non-distensible substantially “C”-shaped structure at each end of the actuator. In some cases the catheter is joined to the retaining rings by, for example, a butt-weld, an ultra-sonic weld, integral polymer encapsulation or an adhesive such as an epoxy.
The actuator body further comprises a central, expandable section 24 located between retaining rings 22a and 22b. The expandable section 24 includes an interior open area 26 for rapid expansion when an activating fluid is supplied to that area. The central section 24 is made of a thin, semi-flexible but relatively non-distensible or flexible but relatively non-distensible, expandable material, such as a polymer, for instance, Parylene (types C, D or N), silicone, polyurethane or polyimide. The central section 24, upon actuation, is expandable somewhat like a balloon-device.
The central section is capable of withstanding pressures of up to about 200 psi upon application of the activating fluid to the open area 26. The material from which the central section is made of is flexible but relatively non-distensible or semi-flexible but relatively non-distensible in that the central section returns substantially to its original configuration and orientation (the unactuated condition) when the activating fluid is removed from the open area 26. Thus, in this sense, the central section is very much unlike a balloon which has no inherently stable structure.
The open area 26 of the actuator is connected to a delivery conduit, tube or fluid pathway 28 that extends from the catheter's lead end to the actuator's proximal end. The activating fluid is supplied to the open area via the delivery tube. The delivery tube often is constructed of Teflon© or other inert plastics. The activating fluid often is a saline solution or a radio-opaque dye.
In some instances, the microneedle 14 is located approximately in the middle of the central section 24. However, as discussed below, this is not necessary, especially when multiple microneedles are used. The microneedle is affixed to an exterior surface 24a of the central section. The microneedle is affixed to the surface 24a by an adhesive, such as cyanoacrylate. Alternatively, the microneedle often is joined to the surface 24a by a metallic or polymer mesh-like structure 30 (See
The microneedle includes a sharp tip 14a and a shaft 14b. The microneedle tip can provide an insertion edge or point. The shaft 14b can be hollow and the tip can have an outlet port 14c, permitting the injection of a pharmaceutical or drug into a patient. The microneedle, however, does not need to be hollow, as it often is configured like a neural probe to accomplish other tasks.
As shown, the microneedle extends approximately perpendicularly from surface 24a. Thus, as described, the microneedle will move substantially perpendicularly to an axis of a lumen into which has been inserted, to allow direct puncture or breach of body lumen walls.
The microneedle further includes a pharmaceutical or drug supply conduit, tube or fluid pathway 14d which places the microneedle in fluid communication with the appropriate fluid interconnect at the catheter lead end. This supply tube often is formed integrally with the shaft 14b, or it often is formed as a separate piece that is later joined to the shaft by, for example, an adhesive such as an epoxy.
In some instances, the needle 14 is a 30-gauge, or smaller, steel needle. Alternatively, the microneedle is microfabricated from polymers, other metals, metal alloys or semiconductor materials. The needle, for example, is made of Parylene, silicon or glass. Microneedles and methods of fabrication are described in U.S. application Ser. No. 09/877,653, filed Jun. 8, 2001, entitled “Microfabricated Surgical Device”, assigned to the assignee of the subject application, the entire disclosure of which is incorporated herein by reference.
The catheter 20, in use, is inserted through an opening in the body (e.g. for bronchial or sinus treatment) or through a percutaneous puncture site (e.g. for artery or venous treatment) and moved within a patient's body passageways 32, until a specific, targeted region 34 is reached (see
During maneuvering of the catheter 20, well-known methods of fluoroscopy or magnetic resonance imaging (MRI) can be used to image the catheter and assist in positioning the actuator 12 and the microneedle 14 at the target region. As the catheter is guided inside the patient's body, the microneedle remains unfurled or held inside the actuator body so that no trauma is caused to the body lumen walls.
After being positioned at the target region 34, movement of the catheter is terminated and the activating fluid is supplied to the open area 26 of the actuator, causing the expandable section 24 to rapidly unfurl, moving the microneedle 14 in a substantially perpendicular direction, relative to the longitudinal central axis 12b of the actuator body 12a, to puncture a body lumen wall 32a. In some instances, it takes only between approximately 100 milliseconds and five seconds for the microneedle to move from its furled state to its unfurled state.
The ends of the actuator at the retaining rings 22a and 22b remain fixed to the catheter 20. Thus, they do not deform during actuation. Since the actuator begins as a furled structure, its so-called pregnant shape exists as an unstable buckling mode. This instability, upon actuation, in some cases produces a large-scale motion of the microneedle approximately perpendicular to the central axis of the actuator body, causing a rapid puncture of the body lumen wall without a large momentum transfer. As a result, a microscale opening is produced with very minimal damage to the surrounding tissue. Also, since the momentum transfer is relatively small, only a negligible bias force is required to hold the catheter and actuator in place during actuation and puncture.
The microneedle aperture, in fact, travels with such force that it can enter body lumen tissue 32b as well as the adventitia, media, or intima surrounding body lumens. Additionally, since the actuator is “parked” or stopped prior to actuation, more precise placement and control over penetration of the body lumen wall are obtained.
After actuation of the microneedle and delivery of the agents to the target region via the microneedle, the activating fluid is exhausted from the open area 26 of the actuator, causing the expandable section 24 to return to its original, furled state. This also causes the microneedle to be withdrawn from the body lumen wall. The microneedle, being withdrawn, is once again sheathed by the actuator.
Various microfabricated devices can be integrated into the needle, actuator and catheter for metering flows, capturing samples of biological tissue, and measuring pH. The device 10, for instance, could include electrical sensors for measuring the flow through the microneedle as well as the pH of the pharmaceutical being deployed. The device 10 could also include an intravascular ultrasonic sensor (IVUS) for locating vessel walls, and fiber optics, as is well known in the art, for viewing the target region. For such complete systems, high integrity electrical, mechanical and fluid connections are provided to transfer power, energy, and pharmaceuticals or biological agents with reliability.
By way of example, the microneedle has have an overall length of between about 200 and 3,000 microns (μm). The interior cross-sectional dimension of the shaft 14b and supply tube 14d often is on the order of 20 to 250 μm, while the tube's and shaft's exterior cross-sectional dimension often is between about 100 and 500 μm. The overall length of the actuator body often is between about 5 and 50 millimeters (mm), while the exterior and interior cross-sectional dimensions of the actuator body can be between about 0.4 and 4 mm, and 0.5 and 5 mm, respectively. The gap or slit through which the central section of the actuator unfurls has in some instances a length of about 4-40 mm, and a cross-sectional dimension of about 50-500 μm. The diameter of the delivery tube for the activating fluid often is about 100 μm. The catheter size often is between 1.5 and 15 French (Fr).
Variations of the present disclosure include a multiple-buckling actuator with a single supply tube for the activating fluid. The multiple-buckling actuator includes multiple needles that can be inserted into or through a lumen wall for providing injection at different locations or times.
For instance, as shown in
Specifically, the microneedle 140 is located at a portion of the expandable section 240 (lower activation pressure) that, for the same activating fluid pressure, will buckle outwardly before that portion of the expandable section (higher activation pressure) where the microneedle 142 is located. Thus, for example, if the operating pressure of the activating fluid within the open area of the expandable section 240 is two pounds per square inch (psi), the microneedle 140 will move before the microneedle 142. It is only when the operating pressure is increased to four psi, for instance, that the microneedle 142 will move. Thus, this mode of operation provides staged buckling with the microneedle 140 moving at time t1, and pressure p1, and the microneedle 142 moving at time t2 and p2, with t1, and p1, being less than t2 and p2, respectively.
This sort of staged buckling can also be provided with different pneumatic or hydraulic connections at different parts of the central section 240 in which each part includes an individual microneedle.
Also, as shown in
Referring now to
Referring now to
As can be seen in
The needle 330 in some cases extends the entire length of the catheter body 312 or, more usually, will extend only partially into the therapeutic or diagnostic agents delivery lumen 337 in the tube 340. A proximal end of the needle can form a sliding seal with the lumen 337 to permit pressurized delivery of the agent through the needle.
The needle 330 will be composed of an elastic material, typically an elastic or super elastic metal, typically being nitinol or other super elastic metal. Alternatively, the needle 330 could be formed from a non-elastically deformable or malleable metal which is shaped as it passes through a deflection path. The use of non-elastically deformable metals, however, is less preferred since such metals will generally not retain their straightened configuration after they pass through the deflection path.
In some cases, the bellows structure 344 is made by depositing parylene or another conformal polymer layer onto a mandrel and then dissolving the mandrel from within the polymer shell structure. Alternatively, the bellows 344 could be made from an elastomeric material to form a balloon structure. In a still further alternative, a spring structure can be utilized in, on, or over the bellows in order to drive the bellows to a closed position in the absence of pressurized hydraulic fluid therein.
After the therapeutic material is delivered through the needle 330, as shown in
In
In
As shown in
Referring to
Actuation of the balloon 12 occurs with positive pressurization. In
As illustrated in
Although the above steps show
To assess the ability of a range of drug or drug combinations and their effects on cell activation, pro-inflammatory cytokine production and cytotoxicity in PDGF-stimulated aortic smooth muscle cells in vitro was examined. A range of concentrations of the mTOR inhibitors sirolimus and temsirolimus and the glucocorticoid dexamethasone were screened, in addition to combinations thereof, in comparison to the mitotic inhibitor paclitaxel.
Cultured human aortic VSMCs (4×105 cells/mL) were grown in complete DMEM in 96 well plates (Corning) and stimulated with PDGF at 10 ng/mL (Peprotech) O/N. Utilizing established protocols for VSMC stimulation mediated by the growth factor, PDGF, drug was incubated at concentration ranges from 0, 1, 10, 100, 500 nanomolar (nM), in the presence or absence of 50 nM dexamethasone, for 12 or 48 hours. Drug-specific effects on cellular metabolic activity, pro-inflammatory cytokine production and cytotoxicity were examined. As a cytotoxic control for disruption of metabolic activity, we used actinomycin D (100 ng/mL or 398 nM). As a negative control, vehicle (5% (v/v) sterile DMSO in saline) was used. Following an 8 hour incubation in the presence of drug and stim (PDGF), MTT substrate was added and incubated for an additional 4 hrs prior to measurement. Absorbance readings were taken using a microtiter plate reader (Tecan). Data represent the average absorbance readings. Error bars represent the standard deviation amongst replicates.
In the presence of PDGF, conversion of MTT substrate into colorimetric product should occur to a significantly higher degree, as compared to media controls. Indeed, in
In yet a further experiment (
Cultured human aortic VSMCs (4×105 cells/mL) were grown in complete DMEM in 96 well plates (Corning) and stimulated with PDGF at 10 ng/mL (Peprotech) O/N. As a negative control (0 nM Drug)), vehicle (5% (v/v) sterile DMSO in saline) was used. Following a 48 hour incubation in the presence of drug and stimulant (PDGF), 50 uL of supernatant from each well was collected and ELISAs were performed to measure TNFα levels. Pro-inflammatory cytokine expression was examined under these conditions by measuring TNFα levels in cell supernatant after 48 h by enzyme-linked immunoassay (ELISA; Peprotech #900-T16, #900-T25). Absorbance readings were taken using a microtiter plate reader (Tecan).
In yet a further experiment (
Cultured human aortic VSMCs (4×105 cells/mL) were grown in complete DMEM in 96 well plates (Corning) and stimulated with PDGF at 10 ng/mL (Peprotech) O/N. As a negative control (0 nM Drug), vehicle (5% (v/v) sterile DMSO in saline) was used. Following a 48 hour incubation in the presence of drug and stimulant (PDGF), 50 uL of supernatant from each well was collected and ELISAs were performed to measure IL6 levels. Pro-inflammatory cytokine expression was examined under these conditions by measuring IL6 levels in cell supernatant after 48 h by enzyme-linked immunoassay (ELISA; Peprotech #900-T16, #900-T25). Absorbance readings were taken using a microtiter plate reader (Tecan).
The percent change in TNFα and IL6 levels compared to vehicle control levels (set as baseline) were also analyzed and are presented in
In yet a further experiment (
Cultured human aortic VSMCs (4×105 cells/mL) were grown in complete DMEM in 96 well plates (Corning) and stimulated with PDGF at 10 ng/mL (Peprotech). Drug (sirolimus; temsirolimus; paclitaxel; dexamethasone) or vehicle control was incubated for 12 hours. At harvest, cells were gently lysed and a Caspase 3 ELISA was performed (Cell Signaling Technologies, Cat #7190). Absorbance readings were taken using a microtiter plate reader (Tecan).
In yet another experiment (
Cultured human aortic VSMCs (4×105 cells/mL) were grown in complete DMEM in 96 well plates (Corning) and stimulated with PDGF at 10 ng/mL (Peprotech). Drug (sirolimus; temsirolimus; paclitaxel; dexamethasone) or vehicle control was incubated for 12 hours. At harvest, plates were centrifuged at 800 rpm in a clinical centrifuge, and supernatant was carefully removed and assayed for lysed cells by measuring the LDH released into the culture media by ELISA, according to manufacturer instructions. Absorbance readings were taken using a microtiter plate reader (Tecan). Temsirolimus, sirolimus, paclitaxel and dexamethasone were also assessed for toxicity using the LDH release assay to measure necrosis and cytolysis in PDGF-treated VSMCs (
In yet a further experiment (
In a porcine model of femoral artery injury, a dose of temsirolimus, dexamethasone, or a combination thereof is administered directly into the tissue around an injured artery through a catheter with a needle. Porcine vascular anatomy is similar to human anatomy, allowing the study of medical equipment intended for use in humans. Porcine vascular pathology allows for the development of stenotic arteries for the study of anti-stenotic or anti-restenotic therapies intended for use in humans.
In twenty-four Yorkshire pigs, the femoral artery in each leg (hind leg) is injured by angioplasty overstretch and followed with either temsirolimus, dexamethasone, combination of both drugs, or control saline injection, for bilateral injury and injection. The angioplasty balloon is selected to be 40-60% larger than the reference diameter of the artery to be injured and is delivered by a catheter to the target injury site by carotid artery access. The angioplasty balloon is inflated to 10-20 atmosphere of pressure three times for 30 seconds each inflation at the target injury site. After the balloon is removed, the Mercator MedSystems Bullfrog® Micro-Infusion Device microneedle catheter is used to deliver either temsirolimus, dexamethasone, combination of both drugs, or control saline by injection into the adventitia and perivascular tissue around the injured artery at the center of each target injury site. The injections are administered under and verified by fluoroscopy. The animals are monitored before, during, and after the procedure, ensuring that all animals survive without adverse incidents until sacrifice.
Temsirolimus preparation. The 25 mg/mL of Torisel® (temsirolimus) is diluted to 10 mg/ml with the supplied diluent and further diluted to 1.0 mg/mL in 0.9% sodium chloride solution. Then, the 1.0 mg/mL temsirolimus is diluted in a ratio of 4 parts temsirolimus to 2 parts contrast medium, IsoVUE 370, and 4 parts 0.9% sodium chloride solution, for a final temsirolimus concentration of 400 μg/ml. This temsirolimus preparation is subsequently administered in temsirolimus-treated group pigs. Similarly, a control solution is prepared by mixing 0.9% sodium chloride solution at 4:1 ratio with a contrast medium, Isovue-370. This control solution is administered in control group pigs.
Dexamethasone preparation. A 10 mg/mL solution of dexamethasone is diluted to 5 mg/mL in 0.9% sodium chloride solution. Then, the 5 mg/mL dexamethasone is mixed at 4:1 ratio with a contrast medium, Isovue-370, for a final dexamethasone concentration of 4 mg/mL. This dexamethasone preparation is subsequently administered in dexamethasone-treated group pigs.
Temsirolimus/dexamethasone combination preparation. The 1.0 mg/mL temsirolimus and the 10 mg/mL dexamethasone solutions are mixed at a 2:2:1 ratio with a contrast medium, Isovue-370, for a final temsirolimus concentration of 0.4 mg/mL, and a final dexamethasone concentration of 4.0 mg/mL. This temsirolimus/dexamethasone preparation is subsequently administered in temsirolimus/dexamethasone-treated group pigs.
Temsirolimus-treated group. Six pigs receive a single dose of temsirolimus (1.5 mL of 400 μg/mL temsirolimus) in the tissue around each 3-cm injured femoral artery segment, for a total of two doses per animal. In each case, all temsirolimus treated animals undergo perivascular infusion into the femoral artery adventitia. Two pigs are sacrificed at each time point of 3 days, 7 days, and 28 days post-procedure, and each pig is analyzed for histopathology, pharmacokinetics, and safety evaluation.
Dexamethasone-treated group. Six pigs receive a single dose of dexamethasone (1.5 mL of 4.0 mg/mL dexamethasone) in the tissue around each injured femoral artery, for a total of two doses per animal. In each case, all dexamethasone treated animals undergo perivascular infusion into the femoral artery adventitia. Two pigs are sacrificed at each time point of 3 days, 7 days, and 28 days post-procedure, and each pig is analyzed for histopathology, pharmacokinetics, and safety evaluation.
Temsirolimus/Dexamethasone combination-treated group. Six pigs receive a single dose of the temsirolimus/dexamethasone composition (1.5 ml of 400 μg/mL temsirolimus/4.0 mg/mL dexamethasone) in the tissue around each injured femoral artery, for a total of two doses per animal. In each case, all temsirolimus treated animals undergo perivascular infusion into the femoral artery adventitia. Two pigs are sacrificed at each time point of 3 days, 7 days, and 28 days post-procedure, and each pig is analyzed for histopathology, pharmacokinetics, and safety evaluation.
Control group. Six pigs serve as control animals. Each pig receives 2 injury sites in femoral arteries, for a total of 12 injury sites among the 6 pigs. Each injury site receives 1.5 ml of 0.9% sodium chloride (saline) diluted 4:1 ratio with contrast medium (Isovue-370). Two pigs are sacrificed at each time point of 3 days, 7 days, and 28 days post-procedure, and each pig is analyzed for histopathology, pharmacokinetics, and safety evaluation.
All treatment and control group animals will successfully receive their respective injection administered directly to the adventitia and perivascular tissues of the femoral arteries. All injection sites except the two control sites will have complete or partial circumferential and longitudinal coverage of the target site by the injection.
Safety Evaluation. Local and systemic toxicity is assessed by clinical observations and clinical pathology either during the survival duration or by analysis of tissues post mortem.
Outcomes. Cellular proliferation as measured by Ki-67 expression, BrdU expression, histopathology, pharmacokinetics, and safety evaluation will be compared for treatment and control groups to ascertain the effect of combination therapy on restenosis in a porcine model.
The general method of Example 7 is followed, with the modification that the researcher wants to study the effects of sequential treatment with both drugs. The combination temsirolimus/dexamethasone-treatment group is removed, the temsirolimus group receives a dexamethasone injection 24 hours after treatment, and the dexamethasone group receives a temsirolimus injection 24 hours after treatment. Outcomes are evaluated in a similar manner, and the effect of sequential treatment is ascertained.
The general method of Example 7 is followed, with the modification that the researcher replaces temsirolimus with paclitaxel, in dosages described herein. Outcomes are evaluated in a similar manner.
The general method of Example 8 is followed, with the modification that the researcher replaces temsirolimus with paclitaxel, in dosages described herein. Outcomes are evaluated in a similar manner, and the effect of sequential treatment is ascertained.
A study was performed to assess the ability of a range of drug or drug combinations to induce cytotoxicity in PDGF-stimulated aortic smooth muscle and endothelial cells in vitro. Cultured human aortic VSMCs (4×105 cells/mL) were grown in complete DMEM in 96 well plates and stimulated with PDGF at 10 ng/mL (Peprotech). A range of concentrations of drug (SIR, sirolimus; TEM, temsirolimus; PAC, paclitaxel; DEX, dexamethasone) and vehicle control or cytotoxic control (ACT D, actinomycin D) were screened (
In the presence of sufficient nutrients and growth factors such as PDGF, cells expressing PDGF receptors, such as VSMCs and ECs up-regulate their cellular metabolism to support growth and proliferation. VSMCs stimulated with 10 ng/mL PDGF showed increased accumulation of metabolite compared to media control and cytotoxic control (the transcription inhibitor actinomycin D at 100 ng/mL). Vehicle control (5% DMSO (v/v) in saline) did not interfere with metabolic activity. Temsirolimus, sirolimus and paclitaxel at higher concentrations had inhibitory activity in the MTT assay. Cellular proliferation in the presence of PDGF showed dose-dependent inhibition of BrdU incorporation compared to vehicle control, and concentrations of temsirolimus, sirolimus and paclitaxel were identified that approached the degree of inhibition of actinomycin D.
Overview. The purpose of the first study was to assess the pharmacokinetic profile of temsirolimus administered directly to the adventitia and perivascular tissues of femoral arteries via the Mercator MedSystems Bullfrog® Micro-Infusion Device. Specific histopathology and histomorphometry endpoints were used to determine efficacy of the temsirolimus therapy.
Design.
The study design included a total of eleven (11) animals; 8 of which received angioplasty overstretch followed by single dose of test article in bilateral superficial femoral arteries administered for a total in-life phase of 1 hour (n=2), 3 days (n=2), 7 days (n=2) and 28 days (n=2) and three additional animals that served as control by undergoing angioplasty overstretch followed by infusion of saline and surviving for 3 days (n=1), 7 days (n=1) and 28 days (n=1). A carotid artery access was utilized for all interventional procedures. In each case, all temsirolimus-treated animals underwent perivascular infusion into the femoral artery adventitia. Animals survived to 1 hour (n=2), 3 days (n=2), 7 days (n=2) and 28 days (n=2), for detailed histopathology and safety evaluation. Following survival to the designated endpoint, animals underwent blood collection, then were euthanized and necropsied followed by histological examination.
A prospective study of a single dose (357 μg in 1.5 ml) of adventitially delivered study drug (Torisel®) was conducted. All doses contained 50% contrast medium for distribution tracking. Each pig received bilateral injections in the mid femoral region. 2 pigs each were sacrificed at 1 hour, 3 days, 7 days and 28 days, for a total of 8 pigs. This was done in two phases: The first phase used to confirm the presence and concentrations of temsirolimus and sirolimus and utilized 3 animals, one each at 1 hour, 3 days and 7 days. The second phase, after confirmation of drug concentrations in the first phase, included the remaining 5 pigs (1 each at 1 hour, 3 days and 7 days and 2 at 28 days). 3 pigs were used as control in which treatment sites were subjected to overstretch injury followed by infusion of saline via the Mercator Bullfrog® catheter and sacrificed at 3 days, 7 days or 28 days (1 pig at each time point).
The injury model was angioplasty overstretch 40-60%, at least 10 atm, 3 inflations of 30 sec each and 30 sec flow. Adventitial delivery was made directly after overstretch injuries. Whole blood samples were taken following each infusion at 5 minutes, 20 minutes, 1 hour, and then 24 hours and upon sacrifice. Whole blood samples were analyzed for circulating temsirolimus and sirolimus concentrations. Arteries were perfused with Lactated Ringers Solution (LRS), extracted, and cut into serial 5 mm sections with every other section fixed in formalin (not perfusion fixed) for immunohistochemical analysis and every other section was frozen for LC/MS/MS analysis of both temsirolimus and sirolimus levels. Histopathology and histomorphometry endpoints were used to determine efficacy of the rapamycin therapy.
Results. All animals successfully received overstretch injury to the superficial femoral arteries followed by administration of the test article or control. All animals in both the test and control groups successfully received the test or control article through the Mercator MedSystems Bullfrog® Micro-Infusion device without any complications and survived to the designated endpoint. The survival period was devoid of any test article/device or procedure related events. The concentration of temsirolimus in the mixture (comprised of 50% temsirolimus and 50% Isovue-370) was 357 μg per 1.5 mL (238 μg/mL), which represents the concentration that the treatment animals received. As all treatment animals received dosages into both femoral arteries, the total dose given to each treatment animal was 714 μg. The mean whole blood baseline temsirolimus levels were below the limits of quantitation (0.200 ng/ml for sirolimus and 0.500 ng/ml for temsirolimus) in all swine prior to dosing. Following dosing, the mean temsirolimus levels were highest at 1 hour after the first injection (32.1±11.0 ng/mL) and decreased to 2.4±1.0 ng/mL within 24 hours. Concentrations continued to decrease to almost non-detectable levels by the third day and by day 7 onwards, all blood analyzed for sirolimus and temsirolimus was found to be below the limits of quantitation.
Similar trends were observed in the sirolimus concentration in the local vascular tissue, but presence of temsirolimus was much more persistent and measured in the tissue up to 28 days post-dosing. Clinical pathology over the course of the survival duration revealed that there were no test device/drug-related effects in hematology. Changes observed in clinical chemistry were considered within expected ranges for biological and/or procedure-related variation. There was no evidence of local toxicity to the treated vessels and no evidence of local vascular irritation upon Torisel® injection with the Mercator MedSystems Micro-Infusion Device. Overall injection of Torisel® with the Mercator MedSystems Bullfrog® Micro-Infusion Device appeared safe in this model. The injection procedure produced no mural injury that could be detectable microscopically. There was no or minor structural injury ascribable to the overstretch angioplasty procedure at 0, 3 and 7 days. There was some evidence of compressive injury in the form of single cell necrosis in the media and/or media hypocellularity. There was no evidence of thrombosis or stenosis. At 28 days two treated vessels showed visible balloon overstretch injury with optimal secondary healing. Mural inflammation was absent or very minimal at all time periods and was associated with the slight mural compressive or overstretch injury noted above.
The treated vessels were fully or nearly fully healed as early as Day 7, generally showing a normal wall and occasionally displaying minimal to mild perivascular or adventitial fibrosis and low severity nonspecific and localized mural inflammation considered to be of no pathological significance. There was complete or near complete re-endothelialization and no or minimal to mild and non-stenosing neointima formation. Ki67 staining indicated cellular proliferation in the control vessel wall peaking on Day 7. Quantitative analysis of Ki67 positive nuclei showed a treatment-related decrease in average proliferation values at Day 3, Day 7 and Day 28. The decrease was substantial and consistent along the vessel length. Staining for smooth muscle actin (SMA) showed SMA positive cells in the adventitia on Day 3 that increased slightly through Day 7 and Day 28 and was primarily associated with myofibroblasts and to a lesser degree with neovascularization. This variation reflected adventitial healing response to vessel injury.
Conclusion. Following temsirolimus administration via the Mercator MedSystems Bullfrog® Micro-Infusion Device, the mean temsirolimus sodium phosphate levels in whole blood were highest at 1 hour post-dose and decreased at 24 hours post-dose. By 3 days post dose, whole blood temsirolimus concentration had fallen to almost non-detectable levels. At 7 and 28 days post-dose, whole blood temsirolimus levels were below the limit of quantitation. At the time of necropsy on study day 28, there were persistent detectable levels of temsirolimus present in the local vascular tissues in the treated swine.
Evaluation of tissues from eleven (11) swine administered either the Torisel® treatment or the control in the adventitia of the peripheral femoral arteries with the Micro-Infusion Catheter (Mercator MedSystems) and euthanized at Day 0 (1 hour post treatment), Day 3, Day 7, and Day 28 post treatment showed no adverse or toxicologically meaningful changes in the treated vessels. There was no or minimal to mild procedural injury that was healed at the end of the study (Day 28) and produced no adverse consequences on the patency or healing of treated vessels. Cellular proliferation was increased on Day 3 in the vessel wall and adventitia and peaked on Day 7 decreasing slightly thereafter. Notably there was a moderate to marked decrease in proliferation indices in Torisel®-treated vessels throughout the vessel wall at all time periods (Day 3, 7 and 28) compared to the respective controls. This decrease was considered to be treatment-related.
The data from the current study indicate that infusion of Torisel® directly into the adventitia of femoral arteries of swine via the Mercator MedSystems Bullfrog® Micro-Infusion device does not produce evidence of local or systemic toxicity assessed by clinical observations and clinical pathology either during the survival duration or by histopathology in the analysis of tissues post mortem.
Overview. The purpose of the second preclinical study was two-fold: (1) Confirm the safety of up to 52 mg Torisel® (temsirolimus) (10 mg per peripheral artery×4 arteries and 4 mg per coronary artery×3 arteries), and 5.2 mg Torisel® (1 mg per peripheral artery×4 arteries and 0.4 mg per coronary artery×3 arteries), as compared to saline; all agents contained 20% contrast medium and were delivered to the perivascular tissues around the coronary and peripheral arteries, and (2) Determine pharmacokinetic profile of temsirolimus and sirolimus in tissue and blood samples after Torisel® (temsirolimus) was delivered to the adventitia and perivascular tissue around femoral and coronary arteries in a porcine model of balloon overstretch artery stenosis.
Methods. Twelve animals were evaluated for tissue safety and pharmacokinetic blood and tissue profiles. The animals received vessel injury induced via oversizing a balloon at ˜20-30% overstretch, then receiving subsequent Bullfrog® catheter delivery of 4 mg/mL Torisel® or saline placebo control into the adventitia and perivascular tissue around the coronary and femoral arteries. Blood samples for pharmacokinetic analysis were taken at 5 minutes, 30 minutes, 1 hour, 24 hours, 72 hours, 7 Days, 28 Days, and termination. Animals were terminated at 90 days. Gross examination of all treated tissues and surrounding structures was performed. One coronary and one femoral artery and surrounding tissues were collected en bloc and flash frozen from each animal (note: one animal did not have a coronary vessel harvested) for pharmacokinetic analysis. Histological analysis was performed on all other collected arterial tissues.
Results.
Endpoint 1: Overall Animal Health. All animals were generally healthy and gained weight for the duration of the study. No adverse treatment related clinical observations were noted. All physical exams were normal throughout the study duration.
Endpoint 2: Tissue Response to the Drug. There was no mortality or significant abnormalities noted in the treated arteries at necropsy. Histologically, all arteries were patent and there were no luminal thrombi or occlusions present in any of the sections examined in the test groups. There were no histological remarkable differences in all graded parameters in the treated vessels between the test groups and the control group at either treatment location. There were thin neointimal formation, medial SMC loss, and medial fibrosis present in the treated coronary and femoral arteries in the control and test groups which most likely were caused by balloon overstretching injury in this animal model. The morphometric measured and calculated parameters were comparable between the control and test groups. The treatment did not create any clinically significant findings in the low and high test groups and therefore, did not raise a safety concern in this animal model. Taken together these findings suggest the safety of the treatments used in this animal model at the 90-day time point.
Endpoint 3: Whole Blood Temsirolimus and Sirolimus Levels. The blood levels for all treated animals peaked at early time points, declined over time to Day 7, and were below LLOQ at 28 days and at termination. In general, observed concentrations were proportional to dose.
Endpoint 4: Homogenized Vascular Tissue Temsirolimus and Sirolimus Levels. The tissue levels for all animals were all below LLOQ.
Conclusion. All animals survived the treatment procedure to 90 days with no related deficiencies. There was no observed vascular toxicity of Torisel® in comparison to saline as assessed with histopathological analysis.
Overview: The purpose of this study was to evaluate the safety and pharmokinetic profile of Torisel® and dexamethasone when delivered in an exaggerated dose to peripheral vasculature in a porcine model of balloon overstretch artery stenosis.
Methods: Four juvenile porcine subjects were used on this study to assess tissue safety and pharmacokinetic profile. The animals received femoral artery injury induced via oversizing a balloon (˜20-30% overstretch). Three animals received subsequent treatment with the Bullfrog® catheter of Torisel® (2 mg/mL) with dexamethasone (6 mg/mL) and contrast medium (20%) into the adventitia and perivascular tissue around the peripheral arteries. The fourth animal received subsequent treatment with the Bullfrog® catheter of saline and contrast medium (20%) into the adventitia and perivascular tissue around the peripheral arteries. Blood samples were taken at baseline, and post-implant 20 minutes, 1 hour, 4 hours, 24 hours, 3 days, 7 days, and just prior to termination for pharmacokinetic assessment. One animal had an early death at day 4 post-implant. The other three animals were survived for 14 days. At day 14, blood was collected, animals were euthanized and a necropsy was performed.
Results.
Endpoint 1: Overall animal health. Three of four animals survived for the study duration and were generally healthy throughout. A fourth animal was found deceased. All surviving animals showed a positive weight gain over the course of the study. The deceased animal was lethargic on day 1. Lethargy continued until the animal was found deceased on day 4. On day 2, blood samples were drawn for analysis, though no conclusive results were obtained. Fever and tachycardia were noted on day 3 prior to the animal being found deceased on day 4. No antemortem diagnosis was made. No significant findings in clinical pathology were noted.
Endpoint 2: Tissue response to the device. Both test and control animals exhibit none to moderate changes in the vessel wall. Perivascular and skeletal muscle changes also ranged from none to moderate in both test and control animals. Mineralization was not noted in control animal tissues examined. However, as mineralization was not previously seen with a dosage of 4 mg/mL temsirolimus, it is considered likely due to the high ethanol concentration in the solution at this dose.
Endpoint 3: Blood temsirolimus and dexamethasone levels (pK assessment). Temsirolimus levels in the blood for all test animals peaked at early time points, declined over time to 7 days and were below the lower limit of quantitation (LLOQ) at termination. The dexamethasone levels in plasma also peaked at early time points and were below LLOQ at the 24-hour time point and after. At 14 days, dexamethasone tissue levels were all <LLOQ, and low levels of temsirolimus were found in both test animals.
Endpoint 4: Homogenized vascular tissue temsirolimus and dexamethasone levels. Levels of temsirolimus (50.3 and 97.5 ng/g, equivalent to 48.8 nM and 94.7 nM, respectively) were found in vascular tissue at term for the two animals treated with the test solution; no temsirolimus was found in the control animal vessels. Dexamethasone levels were <LLOQ (10.0 ng/mL homogenate, equivalent to 100 ng/g in tissue, or 255 nM) in vascular tissue at term for all animals.
Overview. A rabbit study was performed to evaluate the tissue effect of Torisel® (Tem) and dexamethasone (Dex) when delivered as combined or alone to peripheral vasculature in a Watanabe heritable hyperlipidemic (WHHL) rabbit model of balloon overstretch artery stenosis. Each animal was subject to balloon angioplasty and treatment in each of the two (2) external iliac arteries. Four subjects were assigned to each of three groups: Torisel®, dexamethasone, or combination. Three subjects were assigned to each of two groups: control (vehicle delivery) or balloon only. Two New Zealand white rabbits were assigned to treatment with balloon only in one iliac and were naive to any treatment in the other iliac.
Methods. Twenty animals were evaluated for tissue effect of pen-vascular injection of test and control articles. Twenty-two animals underwent procedure on this study, as two animals died early and were replaced. The animals received vessel injury induced via oversizing a balloon (˜20-30% overstretch). Animals were divided into the six groups with varying balloon injury targets and various combinations of test and control articles. The test and control articles were injected into the peri-vascular tissue using the Bullfrog® catheter. Animals were recovered and survived for ˜28 days. At ˜28 days, animals were sacrificed, a gross pathology performed and tissue harvested for histopathology analysis.
Results.
Endpoint 1: Overall animal health (moribundity). A total of 22 animals were utilized on this study. Two animals either died or were euthanized early. The reason for death or euthanasia in these animals were likely due to anesthesia and surgical intervention, but not test/control article related. While there were some instances of inappetance, no animals were noted to experience clinically significant weight loss. Many rabbits were noted as hyperglycemic and hyperlipidemic, however this is consistent with what would be expected from the genetic model of the WHHL rabbits. No other significant health issues were identified throughout the course of the study.
Endpoint 2: Tissue response to the device. The combination therapy (Tem+Dex) group showed less neointimal thickness (47.40±14.84 um) and area (0.19±0.06 mm2) versus temsirolimus, dexamethasone, and vehicle group. The % HHF-35 positive intimal/medial area, as a marker of vascular smooth muscle cell viability, was smaller with combination therapy or temsirolimus alone relative to dexamethasone alone or vehicle group. The number of BrdU and TUNEL positive cells including intima and media showed no statistical difference among these groups.
Twenty of 22 animals survived until 28-day term and 1 of the 20 animals was euthanized per vet orders on day 28 due to acute collapse. For the early death animals, death was related to anesthesia and surgical intervention, not test/control article. All other animals had normal in-life experiences based on the animal model and procedures performed. No vascular toxicity was noted by histopathology for any sample.
The combination therapy (Tem+Dex) group showed less neointimal thickness (47.40±14.84 μm) and area (0.19±0.06 mm2) versus temsirolimus, dexamethasone, and vehicle group. The % HHF-35 positive intimal/medial area, as a marker of vascular smooth muscle cell viability, was smaller with combination therapy or temsirolimus alone relative to dexamethasone alone or vehicle group. The number of BrdU and TUNEL positive cells including intima and media showed no statistical difference among these groups. However, the balloon-only group did not exhibit significant neointima or stenosis, and therefore none of the therapies showed specific benefit over the balloon-only group. The balloon-only group also did not exhibit similar proliferative markers to any of the other groups, suggesting a lack of proliferative injury induction or a different stage of disease in that group of rabbits.
Conclusion. The delivery of Torisel® and dexamethasone to a peripheral vasculature in a Watanabe heritable hyperlipidemic (WHHL) rabbit model of balloon overstretch artery stenosis was found to be safe and effective. Animals survived the study duration of ˜28 days without significant deficiencies related to the test article. Histopathology evaluation of the various groups showed less neointimal thickness and area in animals treated with Torisel and dexamethasone when compared to the Torisel only, dexamethasone only, and vehicle groups.
While preferred embodiments of the present disclosure have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the present disclosure. It should be understood that various alternatives to the embodiments of the present disclosure described herein may be employed in practicing the present disclosure. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.
This application is a divisional of U.S. application Ser. No. 15/990,167 filed on May 25, 2018, which claims the benefit of U.S. provisional patent application No. 62/511,797, filed on May 26, 2017, and U.S. provisional patent application No. 62/624,528, filed on Jan. 31, 2018, which are all hereby incorporated by reference in their entirety.
| Number | Date | Country | |
|---|---|---|---|
| 62624528 | Jan 2018 | US | |
| 62511797 | May 2017 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 15990167 | May 2018 | US |
| Child | 16591467 | US |
