Patent Application
20230055657
The present invention relates to a combination therapy for treating, preventing, or ameliorating conditions mediated by a fibrotic integrin and a farnesoid X receptor (FXR), in particular liver diseases, comprising administering to a subject in need thereof a therapeutically effective amount of an integrin inhibitor and an FXR agonist. Furthermore, the present invention is directed to a pharmaceutical combination comprising an αvβ1 integrin inhibitor and a farnesoid X receptor (FXR) agonist tropifexor, optionally in the presence of a pharmaceutically acceptable carrier, and pharmaceutical compositions comprising them.
Nonalcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease in the Western world. NAFLD is a chronic liver disease (CLD) that was long thought to be a non-progressive form of fatty liver. However, recent clinical and preclinical evidence indicates that NAFLD can progress to more severe non-alcoholic steatohepatitis (NASH) and, as a consequence, patients can develop hepatic fibrosis where there is a persistent inflammation in the liver resulting in the generation of fibrous scar tissue around the liver cells and blood vessels. Eventually, cirrhosis can develop over time whose damage is permanent and can lead to liver failure and liver cancer (hepatocellular carcinoma). Thus, the main stages of NAFLD are: 1) simple fatty liver (steatosis); 2) NASH; 3) fibrosis; and 4) cirrhosis.
Liver transplantation is the only treatment for advanced cirrhosis with liver failure. Estimates of the worldwide prevalence of NAFLD range from 6.3% to 33% with a median of 20% in the general population. The estimated prevalence of NASH is lower, ranging from 3 to 5% (Younossi et al., Hepatology, Vol. 64, No. 1, 2016). NASH is a worldwide problem with growing prevalence over the last few decades. Over the last decade NASH has risen from uncommon to the second indication for liver transplantation in the U.S. It is expected to be the leading cause of transplant by 2024. NASH is highly associated with the metabolic syndrome and Type 2 diabetes mellitus. Furthermore, cardiovascular mortality is an important cause of death in NASH patients.
Development of NASH involves several mechanisms: accumulation of fat in the liver (steatosis), inflammation of the liver, hepatocyte ballooning, and fibrosis. The NAFLD Activity Score (NAS) was developed as a tool to measure changes in NAFLD during therapeutic trials. The score is calculated as the unweighted sum of the scores for steatosis (0-3), lobular inflammation (0-3), and ballooning (0-2).
In chronic liver diseases such as NASH, activated hepatic stellate cells account for the major source of myofibroblasts that drive fibrogenesis (Higashi et al. 2017), and transforming growth factor beta (TGF-β) is a major driver of myofibroblast activation. TGFβ1 is initially secreted with the latency-associated peptide (LAP) that keeps TGFβ1 in an inactive state. One method by which TGF-β1 is converted into its active form is through an interaction between LAP and the αv integrins, including αvβ1. The αvβ1 integrin is an RGD-binding integrin expressed on fibroblasts, and is thought to significantly contribute to TGF-β1 activation in fibrotic liver tissues (Parola et al. 2008, Reed et al. 2015). Pharmacologic inhibition of αvβ1 has been shown to decrease fibrosis in a mouse model of liver fibrosis (Reed et al. 2015). As TGF-β1 signaling is involved in a wide variety of homeostatic processes throughout the body, it is believed that inhibition of the αvβ1TGF-β1 axis specific to fibrotic tissues may allow for a localized, and therefore potentially safer, targeting of TGFβ1 signaling (Henderson et al. 2013, Henderson and Sheppard 2013, Reed et al. 2015).
Farnesoid X Receptor (FXR) is a nuclear receptor activated by bile acids, also known as Bile Acid Receptor (BAR). FXR is expressed in principal sites of bile acid metabolism, such as liver, intestine and kidney, where it mediates effects on multiple metabolic pathways in a tissue-specific manner.
The mode of action of FXR in the liver and intestine is well known, and is described e.g. in Calkin and Tontonoz, (2012) (Nature Reviews Molecular Cell Biology 13, 213-24). FXR is responsible for modulating bile acid production, conjugation and elimination through multiple mechanisms in the liver and intestine. In normal physiology, FXR detects increased levels of bile acids and responds by decreasing bile acid synthesis and bile acid uptake while increasing bile acid modification and secretion in the liver. In the intestine, FXR detects increased bile acid levels, and decreases bile acid absorption and increases secretion of FGF15/19. The net result is a decrease in the overall levels of bile acids. In the liver, FXR agonism increases expression of genes involved in canalicular and basolateral bile acid efflux and bile acid detoxifying enzymes while inhibiting basolateral bile acid uptake by hepatocytes and inhibiting bile acid synthesis.
Furthermore, FXR agonists decrease hepatic triglyceride synthesis leading to reduced steatosis, inhibit hepatic stellate cell activation reducing liver fibrosis, and stimulate FGF15/FGF19 expression (a key regulator of bile acid metabolism) leading to improved hepatic insulin sensitivity. Thus, FXR acts as a sensor of elevated bile acids and initiates homeostatic responses to control bile acid levels, a feedback mechanism that is believed to be impaired in cholestasis. FXR agonism has shown clinical benefits in subjects with cholestatic disorders (Nevens et al., J. Hepatol. 60 (1 SUPPL. 1): 347A-348A (2014)), bile acid malabsorption diarrhea (Walters et al., Aliment Pharmacol. Ther. 41(1):54-64 (2014)) and non-alcoholic steatohepatitis (NASH; Neuschwander-Tetri et al 2015).
The FXR agonist 2-[3-({5-cyclopropyl-3-[2-(trifluoromethoxy)phenyl]-1,2-oxazol-4-yl}methoxy)-8-azabicyclo[3.2.1]octan-8-yl]-4-fluoro-1,3 -benzothiazole-6-carboxylic acid) (see Tully et al, J Med Chem 2017;60:9960-9973) is currently being evaluated in NASH patients with fibrosis (see NCT02855164 study). The compound was disclosed for the first time in WO 2012/087519 (Example 1, compound 1-IB of the table on page 125) and it is also known as tropifexor or LJN452.
Because the pathophysiology of NAFLD and NASH is complex and multiple redundant pathways may be implicated, there is a need to provide treatments for NAFLD, NASH and fibrotic/cirrhotic that can address the different aspects of these complex conditions, while demonstrating an acceptable safety and/or tolerability profile.
The invention provides a pharmaceutical combination comprising, separate or together, at least an αvβ1 integrin inhibitor and at least one additional therapeutic agent, for simultaneous, sequential or separate administration. The invention further provides a medicament comprising the pharmaceutical combination.
In one aspect, the αvβ1 integrin inhibitor is (S)-2-(4-methyltetrahydro-2H-pyran-4-carboxamido)-9-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)nonanoic acid (Compound 1, shown below), a stereoisomer, a tautomer, an enantiomer, a pharmaceutically acceptable salt, a prodrug, an ester thereof or an amino acid conjugate thereof.
In another aspect, the at least one additional therapeutic agent is a non-bile acid derived farnesoid X receptor (FXR) agonist. In another aspect, the non-bile acid derived FXR agonist is 2[3-({5-cyclopropyl-3-[2-(trifluoromethoxy)phenyl]-1,2-oxazol-4-yl}methoxy)-8-azabicyclo[3.2.1]octan-8-yl]-4-fluoro-1,3-benzothiazole-6-carboxylic acid (tropifexor), a pharmaceutically acceptable salt, prodrug and/or ester thereof and/or an amino acid conjugate thereof.
In another aspect, the combination is a fixed dose combination.
In another aspect, the combination is a free combination.
In another aspect, the αvβ1 integrin inhibitor and the at least one additional therapeutic agent can be administered together, one after the other, separately, in one combined unit dose form, or in two separate unit dose forms. The unit dose form may also be a fixed combination.
In another aspect, the pharmaceutical combination is used in the manufacture of a medicament for preventing, delaying or treating a liver disease or disorder.
In one aspect, the invention relates to such pharmaceutical combinations, e.g. fixed or free combinations, e.g. combined unit doses, for use in preventing, delaying or treating a fibrotic or cirrhotic disease or disorder, e.g. a liver disease or disorder. In some aspects, such pharmaceutical combination comprises an αvβ1 integrin inhibitor, e.g. Compound 1, and the at least one additional therapeutic agent, each being in an amount that is jointly therapeutically effective. In another aspect, the at least one additional therapeutic agent is a non-bile acid derived farnesoid X receptor (FXR) agonist. The non-bile acid derived FXR agonist is tropifexor.
The invention provides the use of an αvβ1 integrin inhibitor, e.g. Compound 1, in combination with at least one additional therapeutic agent, e.g. a non-bile acid derived FXR agonist, e.g. fixed or free combination, for the manufacture of a medicament for the prevention or treatment of a liver disease or disorder, e.g. a chronic liver disease or disorder, e.g. cholestasis, intrahepatic cholestasis, estrogen-induced cholestasis, drug-induced cholestasis, cholestasis of pregnancy, parenteral nutrition-associated cholestasis, primary biliary cirrhosis (PBC), primary sclerosing cholangitis (PSC), progressive familiar cholestasis (PFIC), non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), drug-induced bile duct injury, gallstones, liver cirrhosis, alcohol-induced cirrhosis, cystic fibrosis-associated liver disease (CFLD), bile duct obstruction, cholelithiasis, liver fibrosis, renal fibrosis, dyslipidemia, atherosclerosis, diabetes, diabetic nephropathy, colitis, newborn jaundice, prevention of kernicterus, veno-occlusive disease, portal hypertension, metabolic syndrome, hypercholesterolemia, intestinal bacterial overgrowth, erectile dysfunction, progressive fibrosis of the liver caused by any of the diseases above or by infectious hepatitis, e.g. NAFLD, NASH, hepatic fibrosis, hepatosteatis or PBC.
In some aspects of the invention, the invention provides a method of preventing, delaying or treating a liver disease or disorder, in a patient in need therefor, comprising the step of administering a therapeutically effective amount of 1) an αvβ1 integrin inhibitor, e.g. Compound 1, and 2) at least one additional therapeutic agent, e.g. a non-bile acid derived FXR agonist (e.g. tropifexor), wherein the liver disease or disorder is a chronic liver disease or disorder, e.g. cholestasis, intrahepatic cholestasis, estrogen-induced cholestasis, drug-induced cholestasis, cholestasis of pregnancy, parenteral nutrition-associated cholestasis, primary biliary cirrhosis (PBC), primary sclerosing cholangitis (PSC), progressive familiar cholestasis (PFIC), non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), drug-induced bile duct injury, gallstones, liver cirrhosis, alcohol-induced cirrhosis, cystic fibrosis-associated liver disease (CFLD), bile duct obstruction, cholelithiasis, liver fibrosis, renal fibrosis, dyslipidemia, atherosclerosis, diabetes, diabetic nephropathy, colitis, newborn jaundice, prevention of kernicterus, veno-occlusive disease, portal hypertension, metabolic syndrome, hypercholesterolemia, intestinal bacterial overgrowth, erectile dysfunction, progressive fibrosis of the liver caused by any of the diseases above or by infectious hepatitis, e.g. NAFLD, NASH, hepatic fibrosis, hepatosteatotis or PBC.
In some aspects of the invention, the invention provides a pharmaceutical combination for use in preventing, delaying or treating a chronic liver disease or disorder, e.g. NAFLD, NASH, hepatosteatosis, liver fibrosis, cirrhosis, PBC, and steatosis, wherein the combination comprises 1) an αvβ1 integrin inhibitor, e.g. Compound 1, and 2) a non-bile acid derived FXR agonist (e.g., tropifexor).
In some aspects of the invention, the invention provides a pharmaceutical combination comprising 1) an αvβ1 integrin inhibitor, e.g. Compound 1, and 2) a non-bile acid derived FXR agonist (e.g., tropifexor), for use in preventing, delaying or treating NASH.
In some aspects of the invention, the invention provides a pharmaceutical combination comprising 1) an αvβ1 integrin inhibitor, e.g. Compound 1, and 2) a non-bile acid derived FXR agonist, e.g., tropifexor, for use in preventing, delaying or treating liver fibrosis.
In some aspects of the invention, the invention provides a pharmaceutical combination comprising 1) an αvβ1 integrin inhibitor, e.g. Compound 1, and 2) a non-bile acid derived FXR agonist, e.g., tropifexor, for use in preventing, delaying or treating hepatosteatosis.
In some aspects of the invention, the invention provides a pharmaceutical combination comprising 1) an αvβ1 integrin inhibitor, e.g. Compound 1, and 2) a non-bile acid derived FXR agonist, e.g., tropifexor, for use in preventing, delaying or treating hepatocellular ballooning.
In some aspects of the invention, the invention provides a pharmaceutical combination comprising 1) an αvβ1 integrin inhibitor, e.g. Compound 1, and 2) a non-bile acid derived FXR agonist, e.g., tropifexor, for use in preventing, delaying or treating PBC.
The invention provides a method of preventing, delaying or treating a liver disease or disorder, in a patient in need therefor, comprising administering a therapeutically effective amount of each active ingredient in the pharmaceutical combination of the current invention. The pharmaceutical combination comprises 1) an αvβ1 integrin inhibitor, e.g. Compound 1, and 2) a non-bile acid derived FXR agonist, e.g. tropifexor. The liver disease or disorder is a fibrotic or cirrhotic liver disease or disorder, e.g. a liver disease or disorder, e.g. a chronic liver disease or disorder, e.g. NAFLD, NASH, liver fibrosis, cirrhosis and PBC, e.g. NASH, liver fibrosis or PBC.
A method of modulating at least one integrin in a subject, the at least one integrin comprising an αv subunit, the method comprising administering to the subject an effective amount of the pharmaceutical combination comprising administering a therapeutically effective amount of the pharmaceutical combination of the invention. In particular, the integrin being modulated is αvβ1.
The present invention provides a combination of two or more active ingredients that act on two or more distinct modes of NASH pathophysiology. A combination of an αvβ1 integrin inhibitor, e.g. Compound 1, and a non-bile acid derived FXR agonist, e.g. tropifexor, can address the metabolic, anti-inflammatory and antifibrotic pathways involved in NASH. The αvβ1 integrin inhibitor Compound 1 and non-bile acid derived FXR agonist tropifexor impact distinct targets affecting different modes of NASH pathophysiology as evidenced by:
In freshly explanted fibrotic liver tissue obtained at the time of transplant from 5 patients with NASH, the αvβ1 integrin inhibitor Compound 1, showed decreased expression of pro-fibrotic genes, including COL1A1, which encodes the most abundant type of collagen produced in fibrosis, and TIMP1, which encodes the tissue inhibitor of metallopeptidase type 1 (TIMP-1). TIMP-1 is one of the three components of the Enhanced Liver Fibrosis (ELF) score, a noninvasive clinical diagnostic test to assess the likelihood of having clinically significant liver fibrosis.
Compound 1 showed potent and dose-dependent antifibrotic activity in animal models of NASH (CDAHFD) and liver fibrosis (CCl4).
Without wishing to be bound by theory, it is believed from these findings that selective inhibition of integrin αvβ1 by Compound 1 can provide anti-fibrotic benefits in NASH patients with advanced fibrosis.
Tropifexor is designed to address several features of NASH including the buildup of fat in the liver, inflammation and fibrosis.
Compound 1 and tropifexor are potent and highly specific for their respective targets.
The complementary effects of Compound 1 and tropifexor can provide enhanced fibrosis reduction and/or improved clinical benefits in some patient populations.
Various embodiments of the invention are described herein. It will be recognized that features specified in each embodiment may be combined with other specified features to provide further embodiments of the present invention.
The present invention relates to a combination of two or more active ingredients with different Mechanisms of Action (MoA) that provides additional benefits for improving treatment efficacy and response rates.
The present disclosure relates to a pharmaceutical combination comprising, separate or together, at least an αvβ1 integrin inhibitor and at least one additional therapeutic agent, for simultaneous, sequential or separate administration. The invention further provides a medicament, comprising such a combination.
The present disclosure relates to a method of preventing, delaying or treating a liver disease or disorder, in a patient in need therefor, comprising administering a therapeutically effective amount of each active ingredient in the pharmaceutical combination. The pharmaceutical combination comprises 1) an αvβ1 integrin inhibitor, e.g. Compound 1, and 2) a non-bile acid derived FXR agonist, e.g. tropifexor.
The present disclosure relates to a method of modulating at least one integrin in a subject, the at least one integrin comprising an αv subunit, the method comprising administering to the subject an effective amount of the pharmaceutical combination comprising administering a therapeutically effective amount of the pharmaceutical combination of the invention. In particular, the integrin being modulated is 60 vβ1.
In another aspect, the invention provides a method for the treatment of a condition mediated by integrin, in particular a liver disease, in a subject in need thereof, comprising administering to said subject a pharmaceutical combination comprising:
1) an αvβ1 integrin inhibitor, e.g. Compound 1, wherein the αvβ1 integrin inhibitor is administered at a therapeutically effective dose, and
2) a non-bile acid derived FXR agonist, e.g. tropifexor.
The present invention provides a combination of two or more active ingredients that act on two or more distinct modes of NASH pathophysiology. A combination of an αvβ1 integrin inhibitor, e.g. Compound 1, and a non-bile acid derived FXR agonist, e.g. tropifexor, can address the metabolic, anti-inflammatory and antifibrotic pathways involved in NASH. The αvβ1 integrin inhibitor Compound 1 and non-bile acid derived FXR agonist tropifexor impact distinct targets affecting different modes of NASH pathophysiology as evidenced by:
In freshly explanted fibrotic liver tissue obtained at the time of transplant from 5 patients with NASH, the αvβ1 integrin inhibitor Compound 1, showed decreased expression of pro-fibrotic genes, including COL1A1, which encodes the most abundant type of collagen produced in fibrosis, and TIMP1, which encodes the tissue inhibitor of metallopeptidase type 1 (TIMP-1). TIMP-1 is one of the three components of the Enhanced Liver Fibrosis (ELF) score, a noninvasive clinical diagnostic test to assess the likelihood of having clinically significant liver fibrosis.
Compound 1 showed potent and dose-dependent antifibrotic activity in animal models of NASH (CDAHFD) and liver fibrosis (CCl4).
Without wishing to be bound by theory, it is believed from these findings that selective inhibition of integrin αvβ1 by Compound 1 can provide anti-fibrotic benefits in NASH patients with advanced fibrosis.
Tropifexor addresses several features of NASH including the buildup of fat in the liver, inflammation and fibrosis.
Compound 1 and tropifexor are potent and highly specific for their respective targets.
The combined effects of Compound 1 and tropifexor are designed to provide enhanced fibrosis reduction and/or improved clinical benefits in some patient populations.
1a. A pharmaceutical combination for simultaneous, sequentially or separate administration, comprising (i) an αvβ1 integrin inhibitor, e.g. Compound 1; and (ii) a non-bile acid derived FXR agonist, e.g. tropifexor.
2a: A pharmaceutical combination for simultaneous, sequential or separate administration, comprising (i) an αvβ1 integrin inhibitor, e.g. Compound 1, wherein αvβ1 integrin inhibitor is administered at a therapeutically effective dose; and (ii) a non-bile acid derived FXR agonist, e.g. tropifexor.
3a. The pharmaceutical combination according to Embodiment 1a or 2a, wherein the αvβ1 integrin inhibitor, e.g. Compound 1, is in a free form or a pharmaceutically acceptable salt, solvate, prodrug, ester and/or an amino acid conjugate thereof.
4a. The pharmaceutical combination according to Embodiment 3a, wherein tropifexor is in a free form, a pharmaceutically acceptable salt or crystalline form thereof.
5a. The pharmaceutical combination according to Embodiment 4a, comprising about 0.01 mg to about 2 mg of tropifexor.
6a. The pharmaceutical combination according to Embodiment 5a, comprising about 0.01 mg to about 0.3 mg of tropifexor.
7a. The pharmaceutical combination according to Embodiment 5a, comprising about 0.01 mg, about 0.015 mg, about 0.03 mg, about 0.04 mg, about 0.06 mg, about 0.07 mg, about 0.075 mg, about 0.08 mg, about o.09 mg, about 0.1 mg, about 0.14 mg, about 0.15 mg, about 0.2 mg, about 0.25 mg, or about 0.3 mg of tropifexor.
8a. A pharmaceutical combination for simultaneous, sequential or separate administration, comprising: (i) Compound 1; and (ii) tropifexor.
9a. A pharmaceutical combination for simultaneous, sequential or separate administration, comprising: (i) Compound 1; and (ii) from about 0.01 mg to about 2 mg of tropifexor, e.g. from about 0.01 mg to about 1 mg of tropifexor, from about 0.015 mg to about 0.3 mg of tropifexor, from about 0.015 mg to about 0.15 mg of tropifexor, from about 0.03 mg to about 0.2 mg of tropifexor, or from about 0.03 mg to about 0.1 mg of tropifexor.
10a. The pharmaceutical combination according to any one of Embodiments 1a to 9a, comprising Compound 1 in a free form.
11a. The pharmaceutical combination according to any one of Embodiments 1a to 10a, comprising Compound 1 in a zwitterion form.
12a. The pharmaceutical combination according to any one of Embodiments 1a to 11a, wherein the tropifexor is in a crystalline form.
13a. The pharmaceutical combination according to any one of Embodiments 1a to 12a, wherein the tropifexor is in a free form.
14a. The pharmaceutical combination according to any one of Embodiments 1a to 13a, wherein said combination is a fixed combination.
15a. The pharmaceutical combination according to any one of Embodiments 1a to 14a, wherein said combination is a free combination.
16a. A pharmaceutical combination according to any one of Embodiments 1a to 15a, for use in preventing, delaying or treating a condition mediated by integrin, in particular a liver disease or an intestinal disease.
17a. A method of preventing, delaying or treating a liver disease or disorder or an intestinal disease or disorder, in a subject in need thereof, comprising administering a therapeutically effective amount of the pharmaceutical combination according to any one of Embodiments 1a to 16a.
18a. The method according to Embodiment 17a, wherein the liver disease or disorder is a fibrotic or cirrhotic liver disease or disorder, selected from the group consisting of non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), liver cirrhosis, alcohol-induced cirrhosis, cystic fibrosis-associated liver disease (CFLD), liver fibrosis, and progressive fibrosis of the liver caused by any of the diseases above or by infectious hepatitis.
19a. The method according to Embodiment 17a, wherein the liver disease or disorder is non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), primary biliary cirrhosis (PBC), liver fibrosis, or liver cirrhosis.
20a. The method according to Embodiment 17a, wherein the liver disease or disorder is non-alcoholic fatty liver disease, (NAFLD).
21a. The method according to Embodiment 17a, wherein the liver disease or disorder is non-alcoholic steatohepatitis (NASH).
22a. The method according to Embodiment 17a, further comprising resolution of steatohepatitis.
23a. The method according to Embodiment 17a, wherein the liver disease or disorder is liver fibrosis.
24a. The method according to any one of Embodiments 17a to 23a, further comprising improvement in liver fibrosis.
25a. The method according to any one of Embodiments 17a to 24a, further comprising improvement in liver cirrhosis.
26a. The method according to any one of Embodiments 17a to 25a, wherein the non-bile acid derived FXR agonist is administered in the evening.
Definitions
For purposes of interpreting this specification, the following definitions will apply and whenever appropriate, terms used in the singular will also include the plural and vice versa.
As used herein, the term “a”, “an” or the like refers to one or more.
As used herein, the term “about” in relation to a numerical value x means +/−10%, unless the context dictates otherwise.
As used herein, the term “FXR agonist” refers to an agent that directly binds to and upregulates the activity of FXR which may be referred to as bile acid receptor (BAR) or NR1H4 (nuclear receptor subfamily 1, group H, member 4) receptor. FXR agonist may act as agonists or partial agonists of FXR. The agent may be e.g. a small molecule, an antibody or a protein, preferably a small molecule. The activity of a FXR agonist may be measured by several different methods, e.g. in an in vitro assay using the fluorescence resonance energy transfer (FRET) cell free assay as described in Pellicciari, et al. Journal of Medicinal Chemistry, 2002 vol. 15, No. 45:3569-72.
As used herein, the terms “salt” or “salts” refers to an acid addition or base addition salt of a compound of the invention. “Salts” include in particular “pharmaceutical acceptable salts.”
As used herein, the term “amino acid conjugate” refers to conjugates of compounds with any suitable amino acid. Preferably, such suitable amino acid conjugates of a compound will have the added advantage of enhanced integrity in bile or intestinal fluids. Suitable amino acids include but are not limited to glycine, taurine and acyl glucuronide. Thus, the present invention encompasses, for example, the glycine, taurine and acyl glucuronide conjugates of the FXR agonist or αvβ1 integrin inhibitor, i.e., Compound 1 or tropifexor.
As used herein, the term “pharmaceutically acceptable” means a nontoxic material that does not interfere with the effectiveness of the biological activity of the active ingredient(s).
As used herein the term “prodrug” refers to compound that is converted in vivo to the compounds of the present invention. A prodrug is active or inactive. It is modified chemically through in vivo physiological action, such as hydrolysis, metabolism and the like, into a compound of this invention following administration of the prodrug to a subject. The suitability and techniques involved in making and using pro-drugs are well known by those skilled in the art. Suitable prodrugs are often pharmaceutically acceptable ester derivatives.
As used herein, the terms “patient” or “subject” are used interchangeably and refer to a human.
As used herein, the term “treat”, “treating” or “treatment” of any disease or disorder refers in one embodiment to ameliorating the disease or disorder (i.e. slowing or arresting or reducing the development of the disease or at least one of the clinical symptoms or pathological features thereof). In another embodiment “treat”, “treating” or “treatment” refers to alleviating or ameliorating at least one physical parameter or pathological features of the disease, e.g. including those which may not be discernible by the subject. In yet another embodiment, “treat”, “treating” or “treatment” refers to modulating the disease or disorder, either physically, (e.g. stabilization of at least one discernible or non-discernible symptom), physiologically (e.g. stabilization of a physical parameter) or both. In yet another embodiment, “treat”, “treating” or “treatment” refers to preventing or delaying the onset or development or progression of the disease or disorder, or of at least one symptoms or pathological features associated thereof. In yet another embodiment, “treat”, “treating” or “treatment” refers to preventing or delaying progression of the disease to a more advanced stage or a more serious condition, such as e.g. liver cirrhosis; or to preventing or delaying a need for liver transplantation. For example, treating NASH using for example, any of the combinations disclosed herein, may refer to ameliorating, alleviating or modulating at least one of the symptoms or pathological features associated with NASH; e.g. hepatosteatosis, hepatocellular ballooning, hepatic inflammation and fibrosis; e.g. may refer to slowing progression, reducing or stopping at least one of the symptoms or pathological features associated with NASH, e.g. hepatosteatosis, hepatocellular ballooning, hepatic inflammation and fibrosis. It may also refer to preventing or delaying liver cirrhosis or a need for liver transplantation.
As used herein, the term “therapeutically effective amount” refers to an amount of the integrin inhibitor and/or the at least one additional therapeutic agent of the pharmaceutical combination of the invention, individually or in combination, e.g. FXR agonist and/or 60 vβ1 integrin inhibitor, e.g. tropifexor and/or Compound 1, which is sufficient to achieve the respective stated effect. Accordingly, a therapeutically effective amount of a FXR agonist and/or αvβ1 integrin inhibitor, e.g. tropifexor and/or Compound 1, used for the treatment or prevention of a liver disease or disorder as hereinabove defined is an amount sufficient for the treatment or prevention of such a disease or disorder individually or in combination.
By “therapeutic regimen” is meant the pattern of treatment of an illness, e.g., the pattern of dosing used during the treatment of the disease or disorder.
As used herein, a subject is “in need of” a treatment if such subject would benefit biologically, medically or in quality of life from such treatment.
As used herein, the term “liver disease or disorder” encompasses one, a plurality, or all of non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), drug-induced bile duct injury, gallstones, liver cirrhosis, alcohol-induced cirrhosis, cystic fibrosis-associated liver disease (CFLD), bile duct obstruction, cholelithiasis and liver fibrosis.
As used herein, the term NAFLD may encompass the different stages of the disease: hepatosteatosis, NASH, fibrosis and cirrhosis.
As used herein, the term NASH may encompass steatosis, hepatocellular ballooning and lobular inflammation.
As herein defined, “combination” refers to either a fixed combination in one unit dosage form (e.g., capsule, tablet, or sachet), free (i.e. non-fixed) combination, or a kit of parts for the combined administration where an αvβ1 integrin inhibitor of the present invention and one or more “combination partner” (i.e. the at least additional therapeutic agent, such as e.g. a non-bile acid derived farnesoid X receptor (FXR) agonist or a pharmaceutically acceptable salt or solvate thereof, also referred to as or “co-agent”) may be administered independently at the same time or separately within time intervals, especially where these time intervals allow that the combination partners show a cooperative, e.g. synergistic effect.
The terms “co-administration” or “combined administration” or the like as utilized herein are meant to encompass administration of the at least one additional therapeutic agent to a single subject in need thereof (e.g. a patient), and the at least one additional therapeutic agent are intended to include treatment regimens in which the αvβ1 integrin inhibitor and the at least one additional therapeutic agent such as the FXR agonist are not necessarily administered by the same route of administration and/or at the same time. Each of the components of the combination of the present invention may be administered simultaneously or sequentially and in any order. Co-administration comprises simultaneous, sequential, overlapping, interval, continuous administrations and any combination thereof.
The term “pharmaceutical combination” as used herein means a pharmaceutical composition that results from the combining (e.g. mixing) of more than one active ingredient and includes both fixed and free combinations of the active ingredients.
The term “fixed combination” means that the active ingredients, i.e. 1) an αvβ1 integrin inhibitor, e.g. Compound 1 (as defined herein), and 2) the at least one additional therapeutic agent, e.g. a non-bile acid derived FXR agonist, e.g. tropifexor (as defined herein), are both administered to a patient simultaneously in the form of a single entity or dosage.
The term “free combination” means that the active ingredients as herein defined are both administered to a patient as separate entities either simultaneously, concurrently or sequentially with no specific time limits, and in any order, wherein such administration provides therapeutically effective levels of the two compounds in the body of the patient.
By “simultaneous administration”, it is meant that 1) an αvβ1 integrin inhibitor, e.g. Compound 1 (as defined herein), and 2) the at least one additional therapeutic agent, e.g. the FXR agonist, e.g. tropifexor (as defined herein), are administered on the same day. The two active ingredients can be administered at the same time (for fixed or free combinations) or one at a time (for free combinations).
According to the invention, “sequential administration”, may mean that during a period of two or more days of continuous co-administration only one of 1) an αvβ1 integrin inhibitor, e.g. Compound 1 (as defined herein), and 2) the at least one additional therapeutic agent, e.g. the FXR agonist, e.g. tropifexor (as defined herein), is administered on any given day.
By “overlapping administration”, it is meant that during a period of two or more days of continuous co-administration, there is at least one day of simultaneous administration and at least one day when only one of 1) an αvβ1 integrin inhibitor, e.g. Compound 1 (as defined herein), and 2) the at least one additional therapeutic agent, e.g. the FXR agonist, e.g. tropifexor (as defined herein), is administered.
By “continuous administration”, it is meant a period of co-administration without any void day. The continuous administration may be simultaneous, sequential, or overlapping, as described above.
The term “tropifexor” means 2-[3-({5-cyclopropyl-3-[2-(trifluoromethoxy)phenyl]-1, 2-oxazol-4-yl}methoxy)-8-azabicyclo[3.2.1]octan-8-yl]-4-fluoro-1,3 -benzothiazole-6-carboxylic acid (shown below). The term includes a stereoisomer, an enantiomer, in free form, a zwitterion, a polymorph, a pharmaceutically acceptable salt, a solvate, a hydrate, a prodrug, an ester, or an amino acid conjugate thereof; and is also intended to represent unlabeled forms as well as isotopically labeled forms of the compound.
The term “Compound 1” means (S)-2-(4-methyltetrahydro-2H-pyran-4-carboxamido)-9-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)nonanoic acid (shown below). The term includes a stereoisomer, an enantiomer, in free form, a zwitterion, a polymorph, a pharmaceutically acceptable salt, a solvate, a hydrate, a prodrug, an ester, or an amino acid conjugate thereof and is also intended to represent unlabeled forms as well as isotopically labeled forms of the compound.
Unless otherwise specified, the amount of Compound 1 or the additional therapeutic agent refers to the amount of each in a free form.
αvβ1 Integrin Inhibitor
According to an embodiment of the invention, the αvβ1 integrin inhibitor is Compound 1. As defined above, the term “Compound 1” also includes a stereoisomer, an enantiomer, in free form (including a zwitterion), a polymorph, a pharmaceutically acceptable salt, a solvate, a hydrate, a prodrug, an ester, or an amino acid conjugate thereof, e.g. HCl or TFA salt.
In one embodiment, the amino acid conjugate is a glycine conjugate, taurine conjugate or acyl glucuronide conjugate.
In one embodiment, Compound 1 is also intended to represent unlabeled forms as well as isotopically labeled forms of the compound.
Additional Therapeutic Agents or Combination Partners
The terms “additional therapeutic agent” and “combination partner” are herein used interchangeably. A combination of an αvβ1 integrin inhibitor with an FXR agonist can address the metabolic, anti-inflammatory and anti-fibrotic pathways involved in NASH. According to an embodiment of the invention, the at least one additional therapeutic agent is a non-bile acid derived FXR agonist, e.g. tropifexor, in the treatment or prevention of a liver disease or disorder or an intestinal disease or disorder in a subject in need thereof. As defined above, the term “tropifexor” also includes a stereoisomer, an enantiomer, in free form, a zwitterion, a polymorph, a pharmaceutically acceptable salt, a solvate, a hydrate, a prodrug, an ester, or an amino acid conjugate thereof.
In one embodiment, the amino acid conjugate is a glycine conjugate, taurine conjugate or acyl glucuronide conjugate.
Pharmaceutical Compositions
The αvβ1 integrin inhibitor or the at least one additional therapeutic agent each may be used as a pharmaceutical composition with a pharmaceutically acceptable carrier. For example, such a composition may contain, in addition to the αvβ1 integrin inhibitor or an FXR agonist, carriers, various diluents, fillers, salts, buffers, stabilizers, solubilizers, and other materials known in the art. The characteristics of the carrier will depend on the route of administration.
The pharmaceutical composition for use in the disclosed methods may be a free combination of a pharmaceutical composition containing an αvβ1 integrin inhibitor (e.g. Compound 1), and a pharmaceutical composition containing a non-bile acid FXR agonist (e.g. tropifexor), each as described above.
The pharmaceutical composition for use in the disclosed methods may also be a combination pharmaceutical composition in a single dose unit that contains the αvβ1 integrin inhibitor and the at least one additional therapeutic agents for treatment of the particular targeted disorder. For example, a pharmaceutical composition includes the αvβ1 integrin inhibitor and the non-bile acid derived FXR agonist in the treatment or prevention of liver disease or disorder or an intestinal disease or disorder. Such additional therapeutic agents may be included in the combination pharmaceutical composition to produce a synergistic effect with the αvβ1 integrin inhibitor.
Modes of Administration
The pharmaceutical composition of the invention can be formulated to be compatible with its intended route of administration (e.g. oral compositions generally include an inert diluent or an edible carrier). Other non-limiting examples of routes of administration include parenteral (e.g. intravenous), intradermal, subcutaneous, oral (e.g. inhalation), transdermal (topical), transmucosal, and rectal administration.
Diseases
As hereinabove defined, the fibrotic or cirrhotic disease or disorder can be a liver disease or disorder, or renal fibrosis.
In one embodiment of the invention, the pharmaceutical combination (as herein defined) is for the treatment or prevention of a fibrotic disease or disorder, e.g. a liver disease or disorder, e.g. a chronic liver disease, e.g. a liver disease or disorder selected from the group consisting of PBC, NAFLD, NASH, drug-induced bile duct injury, gallstones, liver cirrhosis, alcohol-induced cirrhosis, cystic fibrosis-associated liver disease (CFLD), bile duct obstruction, cholelithiasis, liver fibrosis. In one embodiment of the invention, the pharmaceutical combination (as herein defined) is for the treatment or prevention of fibrosis, e.g. renal fibrosis or liver fibrosis.
According to one embodiment of the invention, the liver diseases or disorders refer to NAFLD, e.g. any stages of NAFLD, e.g. any of steatosis, NASH, fibrosis and cirrhosis.
In one embodiment of the invention, there is provided a pharmaceutical combination of the invention for the improvement of liver fibrosis without worsening of steatohepatitis.
In another embodiment of the invention, there is provided a pharmaceutical combination of the invention for obtaining a complete resolution of steatohepatitis without worsening, e.g. improving, of liver fibrosis.
In another embodiment of the invention, there is provided a pharmaceutical combination of the invention for preventing or treating steatohepatitis and liver fibrosis.
In yet another embodiment of the invention, there is provided a pharmaceutical combination of the invention for reducing at least one of the features of the NAS score, i.e. one of hepatosteatosis, hepatic inflammation and hepatocellular ballooning; e.g. at least two features of the NAS score, e.g. hepatosteatosis and hepatic inflammation, or hepatosteatosis and hepatocellular ballooning, or hepatocellular ballooning and hepatic inflammation.
In a further embodiment of the invention, there is provided a pharmaceutical combination of the invention for reducing at least one or two features of the NAS score and liver fibrosis, e.g. for reducing hepatic inflammation and liver fibrosis, or hepatosteatosis and liver fibrosis or hepatocellular ballooning and liver fibrosis.
In yet a further embodiment of the invention there is provided a pharmaceutical combination for treating or preventing, stage 3 fibrosis to stage 1 fibrosis, e.g. stage 3 and/or stage 2 and/or stage 1 fibrosis.
In one embodiment of the invention, the pharmaceutical combination (as herein defined) is for the treatment or prevention of an intestinal disease or disorder.
Patient Subjects
According to the invention, the patients receiving the combination of the invention can be affected or at risk of a fibrotic disease or disorder, e.g. a liver fibrotic disease or disorder.
Dosing Regimens
Depending on the compound used, the targeted disease or disorder and the stage of such disease or disorder, the dosing regimen, i.e. administered doses and/or frequency, may vary.
The dosing frequency will depend on; inter alia, the phase of the treatment regimen.
According to the invention, tropifexor (as hereinabove defined) is administered at a dose of e.g. about 0.01 mg, about 0.015 mg, about 0.03 mg, about 0.04 mg, about 0.06 mg, about 0.07 mg, about 0.075 mg, about 0.08 mg, about o.09 mg, about 0.1 mg, about 0.14 mg, about 0.15 mg, about 0.2 mg, about 0.25 mg, or about 0.3 mg. Such doses may be for oral administration of tropifexor.
Kits for Treatment of Liver Fibrotic Disease or Disorder
Accordingly, there are provided a pharmaceutical kit comprising: a) an αvβ1 integrin inhibitor, e.g. Compound 1; b) the at least one additional therapeutic agent, e.g. a FXR agonist, e.g. non-bile acid derived FXR agonists, e.g. tropifexor; and c) means for administering the αvβ1 integrin inhibitor and the at least one additional therapeutic agent, to a subject affected by a liver disease or disorder; and optionally d) instructions for use.
In one embodiment of the invention, there is provided a combination package comprising: a) an αvβ1 integrin inhibitor, e.g. Compound 1; and b) at least one individual dose of at least one additional therapeutic agent as hereinabove defined, e.g. at least one individual dose of an FXR agonist, e.g. non-bile acid derived FXR agonists, e.g. tropifexor. The combination package may further comprise instructions for use.
The examples and embodiments described herein are for illustrative purposes only and various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims. All publications, patents, and patent applications cited herein are hereby incorporated by reference for all purposes.
(S)-2-(4-methyltetrahy dro-2H-pyran-4-carboxamido)-9-(5,6,7,8 -tetrahy dro- 1 , 8 -naphthyridin-2-yl)nonanoic acid (Compound 1) may be prepared according to Scheme A below.
Step 1:
To a solution of methyl (S)-2-amino-9-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)nonanoate in DMF was added DIPEA (10 equiv) followed by 4-methyltetrahydro-2H-pyran-4-carboxylic acid (1.1 equiv) and HATU (1.1 equiv). The reaction was allowed to stir at room temperature while monitoring reaction progress by LCMS. When the starting material had been consumed, the reaction was diluted with 1 N NaOH and extracted with EA, washed with brine, dried over sodium sulfate, and concentrated. The crude residue was purified by silica gel chromatography to afford the depicted compound.
Step 2:
To a solution of the depicted ester in an appropriate solvent mixture such as THF/MeOH/H2O or THF/EtOH/H2O was added LiOH (3-5 equiv). The reaction was allowed to stir at room temperature while monitoring reaction progress by LCMS. Upon completion, the reaction was concentrated and purified by reverse phase preparative HPLC to afford the depicted carboxylic acid as the TFA salt. LCMS theoretical m/z=432.2 [M+H]+, found 432.3.
Example 2—Solid Phase Integrin αvβ1 or αvβ6 Binding Assay
Microplates were coated with recombinant human integrin αvβ1 or αvβ6 (2 μg/mL) in PB S(100 μL/well 25° C., overnight). The coating solution was removed, washed with wash buffer (0.05% Tween 20; 0.5 mM MnCl2; in 1×TBS). The plate was blocked with 200 μL/well of Block Buffer (1% BSA; 5% sucrose; 0.5 mM MnCl2; in ×TBS) at 37° C. for 2 h. Dilutions of Compound 1 and recombinant TGFβ1 LAP(0.67 μg/mL) in binding buffer(0.05% BSA; 2.5% sucrose; 0.5 mM MnCl2; in ×TBS) were added. The plate was incubated for 2 hours at 25° C., washed, and incubated for 1 hour with Biotin-Anti-hLAP. Bound antibody was detected by peroxidase-conjugated streptavidin. The IC50 values for the testing compound were calculated by a four-parameter logistic regression.
Example 3—Proximity-Based Integrin αvβ1 or αvβ6 Binding Assay
Compound 1 was tested for αvβ1 or αvβ6 integrin biochemical potency using the ALPHASCREEN® (Perkin Elmer, Waltham, Mass.) proximity-based assay (a bead-based, non-radioactive Amplified Luminescent Proximity Homogeneous Assay) as described previously (Ullman E F et al., Luminescent oxygen channeling immunoassay: Measurement of particle binding kinetics by chemiluminescence. Proc. Natl. Acad. Sci. USA, Vol. 91, pp. 5426-5430, June 1994). To gauge the potency of inhibitors of binding to human integrin αvβ1 or αvβ6, the inhibitor compound and the integrin were incubated together with recombinant TGFβ1 LAP and biotinylated anti-LAP antibody plus acceptor and donor beads, following the manufacturer's recommendations. The donor beads were coated with streptavidin. The acceptor beads had a nitrilotriacetic acid Ni chelator, for binding to a 6×His-tag on human integrin αvβ1 or αvβ6. All incubations occurred at room temperatures in 50 mM Tris-HCl, pH 7.5, 0.1% BSA supplemented with 1 mM each CaCl2 and MgCl2. The order of reagent addition was as follows: 1. αvβ1 or αvβ6 integrin, test inhibitor Compound 1, LAP, biotinylated anti-LAP antibody and acceptor beads were all added together. 2. After 2 hours, donor beads were added. After another 30 min incubation, samples were read.
Example 4—Results of αvβ1 or αvβ6 Integrin Inhibition
The IC50 values obtained for αvβ1 and αvβ6 integrin inhibition for Compound 1 obtained in Examples 2 and 3 are in Table 1 below:
Adult male C57BL/6J mice are housed with ad libitum access to water and food. Mice are fed a HF/NASH diet (40 kcal % fat, 2% cholesterol, 40 kcal % carbohydrate, Research Diets, D09100301 or SSniff Special Diets, supplemented with a fructose-sucrose solution (42 g/L, 55% fructose and 45% sucrose by weight) in drinking water). Age-matched animals are maintained on regular chow (Normal Diet, ND, Kliba Nafag, 3892) and received tap water. Mice are subjected to HF/NASH diet for a total of 20 weeks.
At week 8 of HF/NASH feeding, HF/NASH animals are randomized to treated and untreated groups according to body weight, total lean and fat masses, and liver fat measured by MRI. The study comprises four groups of mice: Group 1: Normal Diet/Water (n=7); Group 2: HF/NASH+tropifexor (n=9); Group 3: HF/NASH+Compound 1 (n=9); and Group 4: HF/NASH+tropifexor+Compound 1 (n=9). Body weight is measured weekly. Fat and lean masses are measured at 0, 4, 7, 14 and 20 weeks of HF/NASH feeding using a mouse body composition nuclear magnetic resonance (NMR) analyzer; and liver fat is assessed at 8, 12, 16 and 20 weeks of HF/NASH feeding using magnetic resonance imaging (MRI).
This study involves 14-day-pregnant C57BL/6 mice. NASH is established by a single subcutaneous injection of 200 μg streptozotocin (Sigma, USA) after birth and feeding with a high fat diet (HFD, 57% kcal fat, CLEA Japan, Japan) ad libitum after 4 weeks of age (day 28±2). Randomization of NASH mice into six groups of 12 mice at 6 weeks of age (day 42±2) and six groups of 12 mice at 9 weeks of age (day 63±2), the day before the start of treatment, respectively. NASH animals are dosed from age 6-9 weeks (Study 1), or from age 9-12 weeks (Study 2) with: vehicle, tropifexor, Compound 1, tropifexor +Compound 1. A non-disease vehicle-control group of 12 mice is included in both Study 1 and Study 2. These animals are fed with a normal diet (CE-2; CLEA Japan) ad libitum.
PK samples are collected and stored at ≤−60° C. Animals are dosed according to the dosing schedule below. Each animal is sacrificed 5 hours after last morning dose on the last day of study treatment.
Dosing:
Tropifexor is prepared in 0.5% (w/v) methylcellulose with 1% Tween® 80 in sterile water for injection (USP).
Compound 1 is prepared in 0.5% (w/v) methylcellulose (400 cPs) aqueous solution containing 0.5% (v/v) polysorbate 80, NF, in reverse osmosis water.
In general, vehicle, monotherapies, and combination therapy are administered once daily.
Measurements:
The following parameters are measured or monitored daily: individual body weight, survival, clinical signs and behavior of mice.
Pharmacokinetic measurements: PK samples are collected from 4 animals per time point per compound. PK samples for Compound 1 are taken at hours 1 and 24 on Days 6 and 10 (n=4 per time point) for both monotherapy and combination groups. Only one PK sample was collected per animal using the first 8 animals per group.
End of Treatment Measurements:
Mice are sacrificed at 9 weeks of age (study 1) or at 12 weeks of age (study 2). The 8 NASH animals that do not receive any treatment or vehicle are sacrificed at week 9 as a ‘baseline’ in order for comparisons of any fibrosis regression events observed in treated animals.
The following samples are collected: plasma, liver (fresh liver samples for gene expression analysis are collected at 5 hr post the last dose for each animal), stool. Organ weight is measured.
The following biochemical assays are performed: Non-fasting blood glucose in whole blood by Life Check (Eidia, Japan); serum ALT by FUJI DRI-CHEM (Fujifilm, Japan); serum triglyceride; serum MCP-1, RANTES (CCL5) and MIP-1α/MIP-1 quantification by a commercial ELISA kit; liver triglyceride by Triglyceride E-test kit (Wako, Japan); liver hydroxyproline quantification by hydrolysis method; histological analyses for liver section; HE staining and estimation of NAFLD Activity score; Sirius-red staining and estimation of fibrosis area (with and without perivascular space subtracted); oil red staining and estimation of fat deposition area; F4/80 immunohistochemistry staining and estimation of inflammation area; alpha-SMA immunohistochemistry staining and estimation of α-SMA positive area Gene expression assays using total RNA from the liver.
Real-time RT-PCR analyses are performed for: MCP-1, MIP-1α/β, RANTES, Emr1, CD68, TGF-β1, CCR2/5, TIMP-1, Cola1A1, TNF, IL-10, MMP-9, α-SMA and CX3CR1/CX3CL1, SHP (small heterodimer partner), BSEP (bile salt export pump), Cyp8b1.
Statistical tests are performed using one-way ANOVA followed by Dunnett's test and the Mann-Whitney test, as appropriate, for the multiple group comparisons. P values <0.05 are considered statistically significant.
The ability of Compound 1 to inhibit the expression of profibrotic genes and decrease biomarkers of fibrosis was measured in precision cut liver slices generated using cirrhotic liver tissues from NASH patient explants and from rodent models of liver fibrosis and NASH.
In precision cut liver slices from 5 cirrhotic NASH patient explants, Compound 1 significantly reduced gene expression of collagen, type 1, alpha 1 (COL1A1) by 39% and also reduced metalloproteinase inhibitor 1 (TIMP1) after two days of treatment (Error! Reference source not found.). Data are mean +/−standard deviation from the 5 cirrhotic NASH patients. DMSO was used as the solvent and utilized at a constant concentration (0.1%) across the different groups. ALK5 was used as a positive control. Compound Lake. significantly reduced the level of FBN-C (26%), a C-terminal fragment of fibronectin (Bager et al. 2010) in culture media. PRO-C1 (34%), PRO-C3 (16%), and PRO-C4 (15%), fragments of the respective collagen subtypes (Leeming et a 2010, Nielsen et al. 2013, Leeming et al 2013), were similarly reduced in culture media with Compound 1 treatment but did not achieve statistical significance.
Example 8—Antifibrotic activity of Compound 1 in a mouse model of liver fibrosis
Antifibrotic activity of Compound 1 was established in an abbreviated, 3-week, murine CCl4 model of liver fibrosis. CCl4 is a hepatotoxin that when injected into mice results in liver fibrosis (Constandinou 2003). Compound 1 was dosed during the final week of injury.
Levels of phosphorylated SMAD3 (pSMAD3)/SMAD3 in the liver, a readout of active TGF-β signaling, were significantly reduced with all doses of Compound 1, demonstrating a reduction in TGF-β signaling. Gene expression analysis indicated a significant reduction in hepatic Col1α1, Col1α1, and Col3α1 expression with all doses of Compound 1. Hepatic OHP concentration was not significantly changed with all doses of Compound 1.
In summary, therapeutic treatment with Compound 1, significantly reduced levels of pSMAD3/SMAD3 in the liver, hepatic collagen gene expression and hepatic OHP concentration.
Example 9—Antifibrotic activity of Compound 1 in a mouse model of NASH
Compound 1 was also demonstrated to be an effective antifibrotic agent in the CDAHFD NASH mouse model. CDAHFD injury is a rodent model of NASH displaying liver fat accumulation, inflammation, and fibrosis (Matsumoto 2013). Three types of studies were performed: 1) prophylactic, Compound 1 in an abbreviated 3-week CDAHFD model; 2) therapeutic, Compound 1 for 6 weeks in the 11- to 12-week CDAHFD model; and 3) Compound 1 for 4 weeks in a 10-week CDAHFD model.
Compound 1 was tested prophylactically in an abbreviated 3-week CDAHFD model at low, medium and high doses across two independent studies. pSMAD3 levels in the liver were decreased by 19% at high dose, suggesting reduced activation of TGF-β. At high dose, Compound 1 significantly reduced hepatic OHP concentrations by ˜30% in both studies. Significantly reduced gene expression of collagens was observed in one of the studies at high dose and expression of Ehhadh, a gene for a peroxisomal bifunctional enzyme involved in fatty acid metabolism, was significantly elevated at high dose in both studies.
Compound 1 was tested therapeutically in 11- to 12-week CDAHFD injury studies at medium, high and highest doses across 3 independent studies. All doses significantly reduced hepatic OHP by up to 38% and pSMAD3 levels by up to 57%. Compound 1 also caused significant reduction in OHP concentration (24%). Collagen gene expression (Col1α1 and Col3α1) was significantly reduced at high and highest doses, as well as gene expression of profibrotic markers of connective tissue growth factor (Ctgf), matrix metalloproteinase 2 (Mmp-2), and Timp1. Gene expression of peroxisomal acyl-coenzyme A oxidase 1 (Acox1) and Ehhadh, which are involved in fatty acid metabolism, was significantly increased. Histological analysis of tissue showed a significant reduction in collagen area and the composite fibrosis score determined through second harmonic generation imaging indicated a significant reduction in quantity and quality of the collagen fibers.
In a 10-week CDAHFD study, the efficacy of Compound 1 was compared to the pan-αv inhibitor CWHM12 (3S)-N-[3-hydroxy-5-[(1,4,5,6-tetrahydro-5-hydroxy-2-pyrimidinyl)amino]benzoyl]glycyl-3-[3-bromo-5-(1,1-dimethyl ethyl)phenyl]β-alanine). pSMAD3 levels were reduced by 40% and 61% and OHP concentrations by 24% and 30% with Compound 1 and CWHM12, respectively. Although pan-αv inhibition with CWHM12 reduced pSMAD and OHP levels, selective αvβ1 inhibition was sufficient for antifibrotic activity.
In summary, treatment with Compound 1, a small molecule antagonist of αvβ1, prophylactically or therapeutically, blocked SMAD3 phosphorylation and significantly decreased OHP levels, collagen gene expression, and collagen deposition examined histologically in the CDAHFD NASH mouse model. These findings were replicated in multiple studies.
Example 10—First-in-human study of the safety, tolerability, PK, and PD of Compound 1
Part A (Single Ascending Dose Study) Part A of the study was a first-in-human, randomized, double-blind, placebo-controlled, parallel-group, single ascending dose study of the safety, tolerability, and PK of Compound 1 in a maximum of 50 healthy male and female (non-childbearing potential) participants. Forty participants will be enrolled in up to 5 sequential cohorts.
Part B (Multiple Ascending Dose Study) Part B of the study is on-going and was initiated after the first 2 cohorts in Part A of the study had been completed. The doses in Part B were determined from Part A of the study and were not higher than the highest dose that was administered in Part A of the study.
Part B of the study is a randomized, double-blind, placebo-controlled, parallel-group, multiple ascending dose study of the PK, PD, safety, and tolerability of Compound 1 administered for 7 days in a maximum of 40 healthy male and female (non-childbearing potential) participants.
Compound 1 is thus far well tolerated in all healthy volunteers.
Example 11—Tropifexor for the treatment of nonalcoholic steatohepatitis: Interim results based on baseline body mass index from Phase 2b study FLIGHT-FXR
The FLIGHT-FXR (NCT02855164) is a Phase 2, randomized, double-blind, multicenter, placebo-controlled trial with an adaptive design to assess the safety, tolerability, and efficacy of tropifexor in patients with NASH (nonalcoholic steatohepatitis). Data from tropifexor 60 μg, tropifexor 90 μg, and placebo arms are provided herein-below.
Patients were divided into two subgroups: Lower BMI subgroup (BMI <30 kg/m2 (Asian) or ≤35 kg/m2 (Non-Asian)) and Higher BMI subgroup (BMI ≥30 kg/m2 (Asian) or ≥35 kg/m2 (Non-Asian)).
The objectives of the study were as follows:
To determine the dose-response relationship of tropifexor on a marker of FXR target engagement in the gut (FGF19) by BMI subgroups over time.
To determine dose-response relationship of tropifexor on markers of hepatic inflammation (alanine aminotransferase [ALT]), target engagement and marker of oxidative stress (gamma-glutamyl transferase [GGT]), and on changes in liver fat content (LFC) measured by magnetic resonance imaging proton density fat fraction (MRI-PDFF) at Week 12 by BMI subgroups.
To determine lipids profile by BMI subgroups.
Results and efficacy of 60 μg tropifexor, 90 μg tropifexor, and placebo
Table 3 (below) shows the results observed in each treatment arms.
†BMI < 30 kg/m2 (Asian) or <35 kg/m2 (Non-Asian);
‡BMI ≥ 30 kg/m2 (Asian) or ≥35 kg/m2 (Non-Asian);
§Measured by MRI-PDFF
Effect of tropifexor on marker of target engagement: FGF19: the assessment of FGF19 was done at Week 6. A dose-response increase in the FGF19 levels was observed 4 hours post-dose compared with pre-dose in both BMI subgroups. At Week 6, the geometric mean percentage changes in FGF19 from pre-dose in the lower BMI subgroup (60 μg of tropifexor=360.2, and 90 μg of tropifexor=585.8) were higher than the mean percentage changes in the higher BMI subgroup (60 μg of tropifexor=276.9, and 90 μg of tropifexor=446.9).
Effect of tropifexor on marker of hepatic inflammation: ALT: A rapid and sustained decline in ALT levels from baseline was observed with 90 μg of tropifexor doses in patients from both BMI subgroups, more marked in the group with lower BMI.
Effect of tropifexor on GGT, a marker of oxidative stress: A dose-response decrease in GGT levels was observed with tropifexor in both BMI subgroups, more marked in the group with lower BMI. At Week 12, the geometric mean percentage change in GGT was higher with 60 μg of tropifexor (−47.0) and 90 μg of tropifexor (−61.3) in the lower BMI versus 60 μg of tropifexor (−38.4) and 90 μg of tropifexor (−48.7) in the higher BMI subgroup.
Effect of tropifexor on liver fat content: At Week 12, the mean percentage change was greater in all arms in the lower BMI subgroup (placebo=−13.1; tropifexor 60 μg=−19.9, and tropifexor 90 μg=−18.8) compared with the higher BMI subgroup (placebo=−5.5; tropifexor 60 μg=−12.9, and tropifexor 90 μg=−11.4). The proportion of patients with an absolute decrease of Liver fat content (LFC) by >5% was higher in the lower BMI subgroup versus the higher BMI subgroup.
Effect of tropifexor on C4: At Week 12, a decrease of 7-hydroxy-4-cholesten-3-one (C4) was observed in all tropifexor treatment groups. This decrease is more obvious in the higher BMI subgroup. However, C4 is subject to diurnal variation, therefore, the influence of BMI on C4 invites further investigation.
As far as the safety of the formulation comprising tropifexor is concerned, the Incidence of adverse events, including pruritus, was comparable between arms. Lipid profiles were comparable in both BMI subgroups. The interim results from the first two parts of this Phase 2b study provide the evidence for target engagement, anti-inflammatory, and antisteatotic effects of tropifexor in both BMI subgroups. However, the effect of tropifexor on ALT, GGT, and LFC was more pronounced in the lower BMI subgroup. The study also showed that the lipid profiles were comparable in both subgroups and that rates of events in the study, including pruritus, were comparable across treatment arms. Consistent trends of lower responses in the higher BMI subgroup, receiving lower dosing by body weight, support testing higher tropifexor doses (e.g. 140 and 200 μg/day).
All references throughout, such as publications, patents, patent applications and published patent applications, are incorporated herein by reference in their entireties.
Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it is apparent to those skilled in the art that certain minor changes and modifications will be practiced. Therefore, the description and examples should not be construed as limiting the scope of the invention.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2020/066079 | 12/18/2020 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 62951615 | Dec 2019 | US |
