The ability of nature to produce small molecules having both structural complexity and biological potency has led to the development of countless number of therapeutic agents such as taxol, penicillin, and quinine, to name a few. Specific organisms found in nature have developed the ability to produce secondary metabolites in response to the challenges and needs that are encountered in their particular environment. In particular, through the random recombination and mutation of existing genetic material, the organism is able, in a combinatorial sense, to generate new biosynthetic enzymes that catalyze the assembly of new organic compounds (see, Verdine “The Combinatorial Chemistry of Nature” Nature, 1996, 384, 11).
Clearly, the ability to generate a diverse array of compounds reminiscent of these natural products would be desirable to increase the arsenal of available small molecules available for testing and use as therapeutic agents. Just as many scientists involved in the discovery and isolation of new natural products sample organisms from many different environments such as coral reefs, deep-sea hydrothermal vents, and tropical rainforests in their quest for structural diversity, both biologists and chemists have been searching for new ways to achieve this structural diversity by manipulating genetic material directly, or by generating novel synthetic pathways, respectively.
For example, certain researchers have attempted to alter the catalytic capability of synthetic enzymes that naturally produce interesting biologically active compounds by making specific changes to genes encoding particular enzymes (see, for example, Cortes et al., Science 268:1487, 1995; Kao et al., J. Am. Chem. Soc. 117:9105, 1995; Donadio et al., Science 252:675, 1991; WO 93/1363; U.S. Pat. No. 5,824,513; WO 98/49315; U.S. Pat. No. 5,652,116; U.S. Pat. No. 5,824,774; WO 98/51695; U.S. Pat. No. 5,795,738; and WO 98/01546). The hope is that, by modifying the gene encoding the synthetic enzyme, the researchers will be able to generate new enzymes with altered synthetic characteristics, which new enzymes will in turn generate new chemical compounds that are related to those produced by the naturally-occurring enzymes, and therefore are likely to have similar desirable biological activities.
Concurrently, several groups have also been interested in generating combinatorial libraries of compounds synthesized using novel synthetic methods. Specifically, in many cases, researchers have developed “biased” libraries, in which all members share a particular characteristic, such as an ability to interact with a particular target ligand, or a characteristic structural feature designed to mimic a particular aspect of a class of natural compounds. For example, a number of libraries have been designed to mimic one or more features of natural peptides. Such “peptidomimetic” libraries include phthalimido libraries (WO 97/22594), thiophene libraries (WO 97/40034), benzodiazopene libraries (U.S. Pat. No. 5,288,514), libraries formed by the sequential reaction of dienes (WO 96/03424), thiazolidinone libraries, libraries of metathiazanones and their derivatives (U.S. Pat. No. 5,549,974), and azatide libraries (WO 97/35199) (for review of peptidomimetic technologies, see Gante, J., Angew. Chem. Int. Ed. Engl. 1994, 33, 1699-1720 and references cited therein). Each of these libraries has provided solid phase synthetic strategies for compounds possessing specific core functionalities, but none achieves the complexity of structure found in natural products, or in other lead compounds prepared through traditional chemical synthetic routes. Complex natural products commonly contain several different functionalities and often are rich in stereochemical complexity. Such diversity and complexity is difficult to achieve if the synthesis is restricted to compounds containing a specific core functionality.
Clearly, there remains a need to develop an efficient and powerful system for the generation of large numbers of compounds having unprecedented stereochemical, structural, topological and functional diversity. The present invention provides a unique method in which both the tools provided by nature and modern organic synthesis can be utilized.
The present invention provides a method for merging combinatorial biosynthesis incorporated above with techniques of synthetic organic chemistry. In general, this method, combinatorial biology, involves 1) providing “starter units”, wherein the starter units are capable of being accepted by the modular biosynthetic enzymatic machinery, and have incorporated therein a “functional handle” capable of reacting with specific functionality present on a solid support; 2) feeding these “starter units” into the modular biosynthetic enzymatic machinery, in vivo or in vitro, to obtain complex template molecules; and 3) further functionalizing the complex template molecules using synthetic organic chemistry to provide a collection of complex “unnatural” natural products having structural, topological, stereochemical and functional diversity.
In a preferred embodiment, the starter units are attached to solid support units prior to feeding the starter units into the modular biosynthetic enzymatic machinery, and thus the support bound starter units are fed into the biosynthetic enzymatic machinery to generate a collection of complex template structures. These template structures thus generated can then be further functionalized using synthetic organic chemistry, or can be further functionalized using any combination of synthetic organic chemistry and reintroduction into the biosythetic enzymatic machinery.
In another preferred embodiment, the starter units are fed to the modular biosynthetic enzymatic machinery prior to being attached to the solid support, and thus template structures are generated. In particularly preferred embodiments specific functionalities can also be incorporated into the template structures via the original starter unit capable of being recognized by an antibody and then purified. Alternatively or additionally, these templates can then be attached to solid support units and further functionalized using synthetic organic chemistry or any combination of synthetic organic chemistry and the biosynthetic enzymatic machinery.
Recognizing the desirability of utilizing both the efficient and powerful methods of natural products biosynthesis and the the diverse repertoire of reactions available in synthetic organic chemistry, a method for merging combinatorial biosynthesis incorporated above with techniques of synthetic organic chemistry is provided. In general, this method, combinatorial biology, involves 1) providing “starter units”, wherein the starter units are capable of being accepted by the modular biosynthetic enzymatic machinery, and have incorporated therein a “functional handle” capable of reacting with specific functionality present on a solid support; 2) feeding these “starter units” into the modular biosynthetic enzymatic machinery, in vivo or in vitro, to obtain complex template molecules; and 3) further functionalizing the complex template molecules using synthetic organic chemistry to provide a collection of complex “unnatural” natural products having structural, topological, stereochemical and functional diversity. As used herein, the term “starter unit” comprises any compound that can be incorporated into the biosynthetic pathway. For example, certain biosynthetic enzymes, such as polyketide synthases, utilize two different classes of “starter units”, specifically “initiator” molecules and “extender“molecules, typically acetates or propionates. For the purposes of the present invention, either category, “initiator” or “extender” qualifies as a “starter” molecule.
As one of ordinary skill in the art will realize, because the starter units have incorporated therein a functional handle, they, or any of the products generated from these starter units, are capable of being attached to solid support units at any stage in the combinatorial biosynthetic pathway. In one preferred embodiment, the starter units are attached to solid support units prior to feeding the starter units into the modular biosynthetic enzymatic machinery, and thus the support bound starter units are fed into the biosynthetic enzymatic machinery to generate a collection of complex template structures. These template structures thus generated can then be further functionalized using synthetic organic chemistry, or can be further functionalized using any combination of synthetic organic chemistry and reintroduction into the biosynthetic enzymatic machinery. In another preferred embodiment, the starter units are fed to the modular biosynthetic enzymatic machinery prior to being attached to the solid support, and thus template structures are generated. In particularly preferred embodiments specific functionalities can also be incorporated into the template structures via the original starter unit capable of being recognized by an antibody and then purified. Alternatively or additionally, these templates can then be attached to solid support units and further functionalized using synthetic organic chemistry or any combination of synthetic organic chemistry and the biosynthetic enzymatic machinery.
Thus, the present invention represents a broadening of the concept of combinatorial biosynthesis to incorporate the advantages of organic synthetic techniques on the solid phase to generate increasingly complex “unnatural” natural products. Various characteristics of the starter units and the reactions utilized in preferred embodiments of the present invention are discussed in more detail below; certain examples of the method of the present invention are also presented.
Biosynthetic Enzymatic Machinery
In principle, the inventive combinatorial biology methods may be applied to any biosynthetic pathway in which the synthetic enzymes will accept inventive starter molecules. In certain embodiments of the invention, the starter molecules are provided to living cells in which the synthetic enzymes are operating, and synthetic reactions in accordance with the present invention are performed in vivo. Alternatively, the biosynthetic pathway may be reproduced in vitro, and the starter molecules may be provided to the synthetic enzymes in that context. Preferred biosynthetic pathways to which the inventive technology may be applied include the animal fatty acid synthase pathway, the polyketide synthase pathway, the peptide synthetase pathway, and the terpene (or isoprenoid) synthase pathway. In certain preferred embodiments of the invention, the naturally-occurring synthetic enzymes are employed and are simply provided with non-natural starter molecules as described herein.
Alternatively, as mentioned above, various researchers have made modifications to certain preferred biosynthetic enzymes that alter their catalytic properties (see, for example, Cortes et al., Science 268:1487, 1995; Kao et al., J. Am. Chem. Soc. 117:9105, 1995; Donadio et al., Science 252:675, 1991; WO 93/1363; U.S. Pat. No. 5,824,513; WO 98/49315; U.S. Pat. No. 5,652,116; U.S. Pat. No. 5,824,774; WO 98/51695; U.S. Pat. No. 5,795,738; and WO 98/01546). Moreover, U.S. patent application Ser. No. 09/225,990, entitled “Improved DNA Cloning”, filed on even date herewith and incorporated herein by reference, describes a powerful system for the production of modified versions of biosynthetic enzymes, and in particular for the production of libraries of modified enzymes.
Preferred embodiments of the present invention utilize such modified enzymes, and preferably libraries of modified enzymes, to catalyze synthetic reactions with inventive starter molecules.
To mention but one particularly preferred embodiment, the system described in the above-mentioned “Improved DNA Cloning” patent application can be utilized to generate a library of class I polyketide synthase enzymes in which a particular “AT” domain responsible for choosing an inventive altered starter molecule has been shuffled to a variety of different positions in the molecule. Exposure of such an enzyme library to the appropriate collection of natural and inventive starter and extender molecules will result in incorporation of inventive molecules at various locations in the polyketide-type compound being synthesized, so that a wide variety of different polyketide-type molecules can be produced by subsequent combinatorialization of the compounds generated by the synthetic enzyme.
Starter Units
As discussed above, the method of the present invention utilizes any combination of enzymatic machinery that can be employed for specific classes of biosynthetic reaction pathways. In determining the specific “starter units” that will be utilized in the synthesis, consideration must also be made to the desired biosynthetic pathway to employ, and thus a desired family of compounds to be synthesized. For example, specific biosynthetic pathways such as polyketide synthases and peptide synthases, utilize particular starter units known to be accepted by the modular enzymes to produce a variety of natural products. In but one example, carboxylic acid building blocks having differing functional moieties such as methyl, ethyl, propyl groups are utilized by modular polyketide synthases to yield topologically, functionally and stereochemically diverse structures such as 6-deoxyerythronolide B and tylactone (see, Khosla, Chem. Rev. 1997, 97, 2577). Similarly, as shown in
Clearly, as mentioned, each of the starter units provided must be capable of being accepted by the enzymes “machinery”, and thus the functionality and structural topology must be compatible with the biosynthetic pathway. In but one example, as shown in
In addition to the selection of suitable starter units that will ultimately be accepted by the enzymatic machinery, it is also necessary to ensure that each of the “starter units” that are fed into the modular biosynthetic enzymatic “machinery” preferably contains a suitable functional “handle” capable of attachment to functionality present on the solid phase.
In an exemplary embodiment, the starter units utilized in the present invention have alkyne functionalities incorporated therein as handles for attachment to the solid support. To illustrate the incorporation of “handles” into the starter units,
An additional important matter to take into consideration when utilizing a starter unit having “handles”, is the number of “handles” that will ultimately be incorporated into the complex template as a result of the biosynthesis. For example, if a simpler (less structurally complex) starter unit is utilized (for example one that is used in successive condensation reactions, as exemplified in the polyketide synthases) a larger number of handles will be incorporated. Thus, if one were to incorporate a handle into a starter unit that is utilized in successive “rounds” by the enzymatic machinery, a template structure would result having many handles incorporated therein. Thus, to overcome this problem, it is preferable to 1) utilize starter units that are only incorporated once, as depicted in
Combinatorial Bioorganic Synthesis
Once the selection of desired starting materials is achieved and functional handles are incorporated therein for attachment to the solid phase and optionally for purification purposes, several variations of the present invention may be employed to obtain “unnatural” natural products.
As shown in
In one particularly preferred embodiment, as shown by pathway A, the selected starter units having the functional handles incorporated therein can be fed directly into the mutated biosynthetic enzymatic machinery. Thus, after exposure to the enzymatic machinery, a unique template compound can be obtained and at this stage can be purified, preferably using an antibody recognition element, or can also be attached to a solid support unit. Subsequently, after attachment to a solid support unit, the template could be reintroduced into the enzymatic machinery (same or different) to thus obtain a modified template. Alternatively, and/or additionally, the template could be utilized in split-pool organic synthesis to generate combinatorial libraries of complex “unnatural” natural products.
In another particularly preferred embodiment, as shown in pathway B, the selected starter units having the functional handles incorporated therein can be attached to the solid support prior to being exposed to the biosynthetic enzymatic machinery. Alternatively, or additionally, a collection of starter units could be generated using combinatorial synthetic organic chemistry. After one or more starter units are fed into the enzymatic machinery, the starter units are transformed into a support bound template structure. As
Referring to the embodiments discussed above, either split-pool or parallel synthesis methods can be employed at each stage of the inventive method to provide a desired collection of compounds. For example, if the starter unit is not initially attached to the solid support a parallel synthesis technique is preferably utilized so that the product resulting from the enzymatic machinery can be identified using spatial encoding methods. Additionally, subsequent identification of compounds using standard methods such as nuclear magnetic resonance spectroscopy or mass spectrometry can also be employed to identify specific compounds. Depending on the size of the library of compounds desired for the synthesis, the identification of individual compounds may be prohibitively time consuming and therefore a spatial encoding method may be more particularly preferred.
As discussed above, in one embodiment of the invention, the template structures are generated in solution, rather than on a solid support unit. In a preferred embodiment, for the generation of a collection of compounds in solution, a parallel synthesis technique is utilized, in which all of the products are assembled separately in their own reaction vessels. In a particularly preferred parallel synthesis procedure, a microtitre plate containing n rows and m columns of tiny wells which are capable of holding a few milliliters of the solvent in which the reaction will occur, is utilized. One of ordinary skill in the art will realize that this particular procedure is most useful when smaller libraries are desired, and the specific wells can provide a ready means to identify the library members in a particular well.
In another more particularly preferred embodiment of the present invention, a solid phase synthesis technique is utilized for the biosynthesis of the templates and the diversified structures, or alternatively for the synthesis of the diversified structures produced from template structures generated in solution as described above. As discussed in detail, the starter units or the template structures are attached to the solid phase directly or though a linking unit, depending on the stage of the procedure a solid phase synthesis is desired. Advantages of solid phase techniques, most particularly at the stage when the template structures are functionalized using synthetic organic techniques, include the ability to more easily conduct multi-step reactions and the ability to drive reactions to completion because excess reagents can be utilized and the unreacted reagent washed away. Perhaps one of the most significant advantages of solid phase synthesis is the ability to use a technique called “split and pool”, in addition to the parallel synthesis technique, developed by Furka. (Furka et al., Abstr. 14th Int. Congr. Biochem., Prague, Czechoslovakia, 1988, 5, 47; Furka et al., Int. J. Pept. Protein Res. 1991, 37, 487; Sebestyen et al., Bioorg. Med. Chem. Lett., 1993, 3, 413.) In this technique, a mixture of related compounds can be made in the same reaction vessel, thus substantially reducing the number of containers required for the synthesis of very large libraries, such as those containing as many as or more than one million library members. As an example, the solid support templates or starter units can be divided into n vessels, where n represents the number species of reagent A, or the number of different biosynthetic pathways created by the shuffling procedure, to be reacted with the template structures or starter units. After reaction, the contents from n vessels are combined and then split into m vessels, where m represents the number of species of reagent B, or the number of different biosynthetic pathways created by the shuffling procedure, to be reacted with the scaffold structures. This procedure is repeated until a desired collection of structures is obtained to yield a library of “unnatural” natural products.
The use of solid phase techniques in the present invention may also include the use of a specific encoding technique. Specific encoding techniques have been reviewed by Czarnik. (Czarnik, A. W., Current Opinion in Chemical Biology, 1997, 1, 60.) As used in the present invention, an encoding technique involves the use of a particular “identifying agent” attached to the solid support, which enables the determination of the structure of a specific library member without reference to its spatial coordinates. One of ordinary skill in the art will also realize that if smaller solid phase libraries are generated in specific reaction wells, such as 96 well plates, or on plastic pins, the reaction history of these library members may also be identified by their spatial coordinates in the particular plate, and thus are spatially encoded. It is most preferred, however for large combinatorial libraries, to use an alternative encoding technique to record the specific reaction history.
Examples of particularly preferred alternative encoding techniques that can be utilized in the present invention include, but are not limited to, spatial encoding techniques, graphical encoding techniques, including the “tea bag” method, chemical encoding methods, and spectrophotometric encoding methods. Spatial encoding refers to recording a reaction's history based on its location. Graphical encoding techniques involve the coding of each synthesis platform to permit the generation of a relational database. Examples of preferred spectrophotometic encoding methods include the use of mass spectroscopy, fluorescence emission, and nuclear magnetic resonance spectroscopy. In a most preferred embodiment, chemical encoding methods are utilized, which uses the structure of the reaction product to code for its identity. Decoding using this method can be performed on the solid phase or off of the solid phase. One of ordinary skill in the art will realize that the particular encoding method to be used in the present invention must be selected based upon the number of library members desired, and the reaction chemistry employed.
Subsequent characterization of the library members, which can include either the scaffolds obtained after the biosynthetic pathway or the complex molecules obtained after diversification using synthetic organic chemistry, can be performed using standard analytical techniques, such as mass spectrometry, Nuclear Magnetic Resonance Spectroscopy, and gas chromatography. One of ordinary skill in the art will realize that the selection of a particular analytical technique will depend upon whether the inventive library members are in the solution phase or on the solid phase.
Reactions at Latent Functionality in the Inventive Templates to Generate “Nonnatural” Compounds
Once the inventive templates have been synthesized as discussed above, diversification reactions may be employed at each of the different latent functionality sites present in the template structures. As mentioned previously, these functionalized templates may then also be reintroduced into the enzymatic machinery for further functionalization and structural changes, as well as be further functionalized using synthetic organic chemistry, until a desired collection of compounds is obtained. One of ordinary skill in the art will appreciate that at any stage the reactivity of a particular functionality present in the template structure must be considered when selecting particular reagents for diversification.
Additionally, for each of the compounds produced by the inventive method, further reactions may be employed to attach biomolecules (such as polysaccharides, nucleic acids, or peptides) or polymers to appropriate functionalities.
One of ordinary skill in the art will realize that the above examples are representative of the reactions that can be used to diversify not only the templates, but also the starter units, of the present invention and are not intended to be exclusive. Rather, all equivalents thereof are intended to be within the scope of the presently claimed invention. A skilled artisan in the field of synthetic organic chemistry will be able to readily identify those reagents capable of reacting to create further diversity at selected sites in the inventive template structures and starter units to generate compounds and libraries of compounds reminiscent of natural products. The inventive method is particularly useful for the generation of such compounds because it incorporates the efficiency and creativity of a method for manipulating the enzymatic reactions that produce such complex structures in nature with the arsenal of reactions available using synthetic organic techniques. For example, the generation of complex templates can be difficult utilizing only synthetic organic techniques, however, nature efficiently and elegantly provides these templates. Combining this with synthetic organic chemistry enables the use of reactions, such as palladium catalyzed reactions, that are not available in nature and thus the best of both systems can be utilized to generate compounds having unprecedented structural, topological, stereochemical and functional diversity.
Uses
The methods, compounds and libraries generated by the method of the present invention can be utilized in various disciplines. For example, the complex molecules generated in the method of the present invention may modulate the biological activity of a biological target, such as a protein, nucleic acid, lipid or combination thereof. In one preferred embodiment, the compounds generated by the method of the invention are utilized in chemical genetics assays to alter, i.e. inhibit or initiate, the action of such biological molecules. Alternatively or additionally, the compounds may be used in in vitro assays, or any other system that allows detection of a chemical or biological function.
In a particularly preferred embodiment of the invention, one or more inventive compounds is contacted with a biological target having a detectable biochemical activity. Such biological targets include, for example, enzymes, receptors, subunits involved in the formation of multimeric complexes. Such multimeric complex subunits may be characterized by catalytic capabilities (such as, for example, an ability to catalyze substrate conversion), or may alternatively be primarily active in binding to one or more other molecule. The biological target can be provided in the form of a purified or semi-purified composition, a cell lysate, a whole cell or tissue, or even a whole organism. The level of biochemical activity is detected in the presence of the compound, and a statistically significant change in the biochemical activity, relative to the level of biochemical activity in the absence of the compound, identifies the compound as a modulator, e.g. inhibitor or potentiator of the biological activity of the target protein. In some cases, particularly where assays are done on whole cells or organisms, the effect of the chemical compound may be to alter the amount, in addition to or instead of the activity, of the particular biological target. “Modulators”, therefore, are chemical compounds that alter the level of activity of a particular target molecule.
In one particularly preferred embodiment of the present invention, multiple compounds are assayed simultaneously in a high-throughput format, preferably allowing simultaneous analysis of at least 500,000 compounds, preferably at least 1,000,000 compounds, and most preferably at least or more than 2,000,000 compounds.
As discussed above, once a specific desired effect on a biological target has been associated with a particular compound of the inventive library, the compounds of the present invention may be utilized as a therapeutic agent for a particular medical condition. A therapeutic agent for use in the present invention may include any pharmacologically active substances that produce a local or systemic effect in animals, preferably mammals, or humans. The term thus means any substance intended for use in the diagnosis, cure, mitigation, treatment or prevention of disease or in the enhancement of desirable physical or mental development and conditions in an animal or human. The therapeutic agent may be administered orally, topically or via injection by itself, or additionally may be provided as a pharmaceutical composition comprising the therapeutic agent and a biologically acceptable carrier. The inventive compositions can be, but are not limited to aqueous solutions, emulsions, creams, ointments, suspensions, gels, and liposomal suspensions. Particularly preferred biologically acceptable carriers include but are not limited to water, saline, Ringer's solution, dextrose solution and solutions of ethanol, glucose, sucrose, dextran, mannose, mannitol, sorbitol, polyethylene glycol (PEG), phosphate, acetate, gelatin, collagen, Carbopol, and vegetable oils. It is also possible to include suitable preservatives, stabilizers, antioxidants, antimicrobials, and buffering agents, for example including but not limited to BHA, BHT, citric acid, ascorbic acid, and tetracycline. The therapeutic agents of the presently claimed invention may also be incorporated or encapsulated in a suitable polymer matrix or membrane, thus providing a sustained-release delivery device suitable for implantation near the site to be treated locally.
As one of ordinary skill in the art will realize, the amount of the therapeutic agent required to treat any particular disorder will of course vary depending upon the nature and severity of the disorder, the age and condition of the subject, and other factors readily determined by one or ordinary skill in the art.
In alternative embodiments, the compounds and libraries of the present invention may also be used for the development of cosmetics, food additives, pesticides, and lubricants to name a few. Furthermore, the compounds and libraries of the present invention may also be used for the development of novel catalysts and materials. For example, the inventive compounds may be useful as ligands for transition metal catalysts and the inventive libraries may be useful for the rapid identification of novel ligands. These compounds and libraries of compounds may also function by acting in concert with a particular transition metal catalyst to effect a particular desired chemical reaction. Additionally, the inventive compounds and libraries of compounds are also useful in the area of materials science. Because of the reactive moieties present in these compounds, molecules such as lipids and other polymeric materials may be attached and thus generate potentially important biomaterials.
One of ordinary skill in the art will realize that the present invention is not intended to be limited to the abovementioned uses, but rather may be employed in many contexts and disciplines.
The present application claims priority to currently pending U.S. patent application Ser. No. 09/478,263, filed Jan. 5, 2000, which claims priority to Provisional Application U.S. Ser. No. 60/114,909, filed Jan. 5, 1999, the entire contents of each of which are incorporated herein by reference.
Number | Date | Country | |
---|---|---|---|
60114909 | Jan 1999 | US |
Number | Date | Country | |
---|---|---|---|
Parent | 09478263 | Jan 2000 | US |
Child | 11763966 | Jun 2007 | US |