Claims
- 1. A process for preparing a compound of formula 1:
- 2. A process of claim 1, wherein the organism is Saccharopolyspora erythraea and which may optionally contain an effectively integrated plasmid capable of directing the biosynthesis of compounds of formula 1; said plasmid may optionally contain a Type II PKS promoter/activator gene.
- 3. A process of claim 2, wherein the organism Saccharopolyspora erythraea is selected from strains NRRL 2338, 18643 or 21484 which may optionally contain an effectively integrated plasmid capable of directing the biosynthesis of compounds of formula 1; said plasmid may optionally contain the act promoter and its cognate activator gene actII-orf4.
- 4. A process of claim 3, wherein the optionally effectively integrated plasmids is pAVLD, pIG1, pND30, pCJR26, pCJR49, pC-AT12, pc-ATX or other similar constructs.
- 5. A process of claim 3, wherein the organism is S. erythraea ERMD1, S. erythraea NRRL 2338/pIG1, S. erythraea NRRL 2338/pND30, or other similar tranformants.
- 6. A process for preparing a compound of formula 2:
- 7. A process of claim 6, wherein the organism is Saccharopolyspora erythraea and which may optionally contain an effectively integrated plasmid capable of directing the biosynthesis of compounds of formula 2; said plasmid may optionally contain a Type II PKS promoter/activator gene.
- 8. A process of claim 7, wherein the organism Saccharopolyspora erythraea is selected from strains NRRL 2338, 18643 or 21484 which may optionally contain an effectively integrated plasmid capable of directing the biosynthesis of compounds of formula 2 said plasmid may optionally contain the act1 promoter and its cognate activator gene actII-orf4.
- 9. A process of claim 8, wherein the optionally effectively integrated plasmids is pAVLD, pIG1, pND30, pCJR26, pCJR49, pC-ATI2, pc-ATX or other similar constructs.
- 10. A process of claim 8, wherein the organism is S. erythraea ERMD1, S. erythraea NRRL 2338/pIG1, S. erythraea NRRL 2338/pND30, or other similar tranformants.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PCT/US98/14911 |
Jul 1998 |
WO |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. Ser. No. 08/846,247 filed 30 Apr. 1997 which is a continuation-in-part of U.S. Ser. No. 08/486,645 filed 7 Jun. 1995 which is continuation-in-part of U.S. Ser. No. 08/238,811 filed 6 May 1994. Priority is claimed under 35 USC §120. Priority is also claimed under 35 USC 119(e) with respect to U.S. Provisional application 60/076,919 filed 5 Mar. 1998. The disclosures of these applications are incorporated herein by reference.
REFERENCE TO GOVERNMENT FUNDING
[0002] This work was supported in part by a grant from the National Institutes of Health, CA66736. The U.S. government has certain rights in this invention.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60003338 |
Jul 1995 |
US |
Continuations (2)
|
Number |
Date |
Country |
Parent |
09073538 |
May 1998 |
US |
Child |
10340139 |
Jan 2003 |
US |
Parent |
09164306 |
Oct 1998 |
US |
Child |
09311756 |
May 1999 |
US |
Continuation in Parts (8)
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Number |
Date |
Country |
Parent |
08846247 |
Apr 1997 |
US |
Child |
09073538 |
May 1998 |
US |
Parent |
08486645 |
Jun 1995 |
US |
Child |
08846247 |
Apr 1997 |
US |
Parent |
08238811 |
May 1994 |
US |
Child |
08486645 |
Jun 1995 |
US |
Parent |
08164301 |
Dec 1993 |
US |
Child |
08238811 |
May 1994 |
US |
Parent |
08123732 |
Sep 1993 |
US |
Child |
08164301 |
Dec 1993 |
US |
Parent |
09311756 |
May 1999 |
US |
Child |
10340139 |
Jan 2003 |
US |
Parent |
08896323 |
Jul 1997 |
US |
Child |
09164306 |
Oct 1998 |
US |
Parent |
08675817 |
Jul 1996 |
US |
Child |
08896323 |
Jul 1997 |
US |