Patent Application
20050129712
The present invention relates to novel, combined DNA/Protein vaccine compositions comprising at least one nucleic acid molecule encoded antigen and at least one protein antigen adjuvanted in a mineral-based adjuvant so as to enhance the immunogenicity of both in a single vaccine formulation.
DNA vaccination is an important novel immunization strategy to specifically prime humoral and cellular immune responses (S. Gurunathan et al., Annu Rev. Immunol. (2000) 18:927-74). With DNA vaccines based on injection of nucleotides alone 50-100 μg nucleotide is required to elicit a potent immune response against the antigen encoded by the DNA. It is not known how large a proportion of the DNA that is de facto transcribed into protein and it is not clear how much protein that it subsequently gives lead to. It has been shown, however, that no or barely detectable immune responses are detected when <10 μg plasmid DNA is injected into mice.
Currently, DNA vaccines are not yet on the market. Limitations that prevent the introduction of DNA vaccination into clinical practice include the conspicious absence of techniques that can deliver low doses of a DNA vaccine in a sufficiently immunogenic form to a wide range of animals and to man. It will be clear that the development of techniques which would lead to the elicitation of a potent immune response after administration of significantly lower doses of DNA nucleotide would attract both scientific and economic interest among vaccine producers.
In recent literature various adjuvants have been proposed to enhance the immunogenicity of DNA vaccines. For example, the interrelated references WO 00/02591, WO 98/35562, and S. Wang et al., Vaccine 18:1227-1235 (2000) disclose vaccine formulations comprising nucleic acid molecules and an aluminium phosphate-based or calcium phosphate-based adjuvant provided in a biologically effective concentration so as to improve induction of an immune response subsequent to vaccination. The formulations with calcium- or aluminum phosphate adjuvants prior to injection have been shown to facilitate delivery of DNA vaccines, since antibody titers are increased by 10- to 100-fold and the immunogenic dose of DNA is decreased by 10-fold. Although the mechanism by which aluminium phosphate or aluminium hydroxyphosphate exerts this adjuvant effect is not clear, the adjuvants have an extensive record from use in practical vaccination.
WO 99/51269 discloses a DNA vaccine containing a naked DNA incorporating and expressing in vivo a nucleotide sequence encoding an antigenic polypeptide and at least an adjuvant compound selected from the class of (meth)acrylic polymers and copolymers of maleic anhydride and alkenyl derivatives, preferably a carbomer or an EMA®.
WO 02/03961 discloses a nucleic acid delivery system comprising nucleic acid molecules encapsulated in biodegradable microspheres, suitable for delivering DNA vaccines, which comprises an adjuvant for modulating the immunostimulatory efficacy said adjuvant comprising an aminoalkyl glucoaminide-4-phosphate (AGP).
WO99/30733 discloses a method to enhance an immune response based on a combination of polynucleotide and polypeptide. Pharmaceutical compositions comprising DNA+polypeptide may contain a specially prepared aluminium hydroxide (Al(OH)3). The protein antigen was adsorbed onto aluminium hydroxide (Example 8) and subsequently co-acervated with polycaprolactone to create a complex form with slow-release of antigen from the specially treated Al(OH)3. This complex was then mixed with the DNA in the ratio 2 μg protein to 10 μg DNA prior to use. No other approaches to adjuvantation of the peptide-nucleotide combination using other mineral-based adjuvants were investigated.
WO 97/28818 discloses vaccinations and vaccines comprising a nucleic acid encoding a first epitope and a peptide encoding a second epitope, in particular in vitro transfection of APC's (Antigen Presenting Cells) with DNA encoded antigen (and not muscular tissue which is the normal target tissue for DNA immunization) and the simultaneous presence of peptide antigen as well as DNA encoded and transcribed antigen in APC's was demonstrated. Several vectors and delivery systems were discussed and a speculation was made (p. 42) on the possible use of a wide range of adjuvants, but no experimental data for the use of any adjuvant was provided.
Conventional vaccines allow the combination of different antigens, but only within the group of either adjuvanted vaccine components or within the group of non-adjuvanted live-attenuated vaccine relevant organisms. The former group which is adjuvanted by mineral salts primarily gives lead to a Th2 response which is protective for the mentioned diseases, whereas similar adjuvantation could have an inhibitory effect on the immune response necessary for protection against the live attenuated vaccine components (E. B. Lindblad et al., Infect. Immun. 65(2):623-629 (1997)). This dichotomy currently limits the possibilities of making combination vaccines where diseases from the two groups, respectively, are represented. The diseases covered by the live attenuated vaccines are often requiring a Th1 type immune response as protecting against these diseases does not depend upon an antibody response alone.
There is still a need for further investigation in order to improve the immuno-genicity of DNA vaccines. It is noted in this connection that as far as the inventors are aware the possibility of combining DNA nucleotide-based vaccines with conventional protein or adsorbed protein-based vaccines for establishing an efficient combination of these two technologies has hitherto not been explored, especially not in relation to the use of negatively-charged mineral-based adjuvants.
The DNA vaccine technology has the potential of inducing a Th1 type response necessary for protection against those diseases which are at present prevented by the group of live attenuated vaccines. The present invention provides a combination of at least one DNA vaccine with one or more classically adjuvanted Th2 stimulating vaccines, thereby overcoming such limitations of preparing an extended programme of combination vaccines.
The present invention provides a combined DNA/Protein antigen vaccine composition suitable for administration to a vertebrate host, including man, which comprises:
In a preferred embodiment of the invention said mineral-based, negatively charged adjuvant is an adjuvant selected from the group of aluminium phosphate-based and aluminium hydroxyphosphate-based adjuvants.
In another embodiment of the invention said mineral-based, negatively charged adjuvant is an adjuvant selected from the group of calcium phosphate-based and calcium hydroxyphosphate-based adjuvants.
Suitable polynucleotide vaccine components which can be used in the vaccine composition according to the invention include components from vaccines where the classical aluminium adjuvants are considered inadequate to elicit protection, for example intracellular parasitic bacteria like Mycobacterium sp. and Leishmania sp., and viruses like cytomegalovirus (CMV), HTLV-I, HIV, hepatitis C and D, influenza, measels, rubella, and tumour-associated antigens.
Suitable model protein antigens may range from acidic IEP proteins, such as bovine serum albumin and human serum albumin, to basic IEP proteins, such as lysozyme.
Suitable vaccine protein antigens include but are not limited to detoxified toxins as well as other vaccine relevant antigens at present used in conventional vaccines against e.g. tetanus, diphtheria, botulinus poisoning, pertussis, inactivated poliomyelitis, hepatitis A and B, and the like.
In yet another preferred embodiment said mineral-based, negatively charged adjuvant is pre-incubated by said at least one protein antigen vaccine component prior to being formulated with said polynucleotide vaccine component to a vaccine composition according to the present invention.
In a further aspect of the invention the use of a mineral-based, negatively charged adjuvant is provided as a component in a combined DNA/Protein based vaccine composition as herein defined.
In another aspect of the invention a kit is provided comprising a vaccine composition as defined above, in a unit dose form for administration to a vertebrate recipient, including man.
In yet another aspect of the invention a method of inducing an immune response in a vertebrate host is provided which comprises introducing a vaccine composition as defined above into said vertebrate host.
These and other embodiments of the present invention will be outlined in more detail in the following detailed description.
The term “polynucleotide vaccine” (PNV) which is identical to “DNA vaccine”, as used herein, is meant to indicate a DNA vector containing a gene encoding a viral, bacterial, parasitic or tumor antigen which has been shown to express that respective antigen subsequent to administration to the vertebrate, for example by intramuscular injection.
The term “model protein antigen” as used herein is meant to define a protein which is not derived from an infectious microorganism which may cause one or more diseases. Hence a model protein antigen is not included in a vaccine preparation with the aim of eliciting a protective immune response towards the model protein itself.
The term “conventional vaccines”, as used herein, is meant to define well-established vaccines, whether they are based on (1) live, attenuated vaccine components (not adjuvanted), or (2) killed inactivated vaccine components, killed whole cells, purified subunit or peptide components (which are adjuvanted), using e.g. mineral adjuvants.
The term “vaccine protein antigen” as used herein, refers to an antigen, regardless whether it is a protein-containing cell fragment, a purified protein, a synthetic peptide or whether in its nature it consists of amino acids only or of amino acids in combination with other biological molecules, like carbohydrates or lipids, derived from an infectious organism against which the vaccine is intented to protect. The purpose of including the vaccine protein antigen is to induce specific and protective immunity towards the infection caused by the organism that the vaccine protein relevant was derived from.
The term “adjuvant”, as used herein, refers to one or more compounds that enhance the immune response against the antigenic components of the vaccine formulation or helps delivering the nucleotide or provides a stimulative effect for the immune response to transcription products of the injected DNA nucleotides.
The term “negatively charged mineral-based phosphate adjuvant”, as used herein, refers to preformed aluminium phosphate or aluminium hydroxyphosphate gel adjuvants, and the like, which are characterized by having an acidic point of zero charge (PZC). It also refers to a formulation by which preformed aluminium hydroxide adjuvant (which normally has an alkaline PZC) after its formation is incubated with phosphate ions to form a complex with these by which process it also—subsequently—achieves an acidic PZC. It also refers to calcium phosphate or calcium hydroxyphosphate based adjuvants.
The term “point of zero charge” or “PZC”, as used herein refers to the specific pH value at which a given mineral-based adjuvant has no net charge. As such the PZC is analogous to the isoelectric point (IEP) of a protein as known from protein chemistry. At pH values above the PZC the net charge of the mineral-based adjuvant is negative and below the PZC the net charge of the mineral-based adjuvant is positive.
The present invention is based on the surprising finding, after extensive research and experimentation, that aluminium phosphate or alumium hydroxyphosphate-mediated enhancement of the immunogenicity of DNA vaccines was further improved by pre-incubating aluminium phosphate or aluminium hydroxyphosphate with at least one suitable protein. It was also found that aluminium phosphate and aluminium hydroxy-phosphate and their calcium counterparts can be used to combine protein- and DNA-based vaccination to prime an enhanced and differentiated specific immunity.
The invention relates in one aspect to a novel vaccine formulation comprising nucleic acid molecules and a mineral-based, negatively charged adjuvant delivered in a biologically effective concentration so as to promote the effective induction of an immune response directed towards one or more specific antigens encoded by the nucleic acid molecule(s). According to this aspect the adjuvant, when delivered in conjunction, e.g. pre-incubated or pre-mixed with a suitable protein, seems to have a different appearance as compared to the original vaccine formulations on which this improvement is based which have been extensively described, inter alia in WO 00/2591, the disclosure of which is incorporated herein by reference. Apart from the improvement defined above, any aspect of said disclosure including definitions, preparation of the pharmaceutical formulations, selection and amounts of ingredients, methods of inducing an immune response in an vertebrate host using the pharmaceutical formulations, way of delivery of the formulations to the vertebrate host, the diseases or disorders to be treated by the formulations, etc., apply equally to the present invention.
The present invention is further based on the surprising finding that the enhancement is mediated by the negatively charged mineral-based phosphate adjuvant (as exemplified by, but not limited to aluminium phosphate and aluminium hydroxy phosphate), with a suitable protein, as exemplified by either a model protein, such as an acidic IEP protein, for example bovine serum albumine (BSA) having low adsorption affinity, or a basic IEP protein, for example hen egg lysozyme (HEL) having high adsorption affinity, or, alternatively, a vaccine-relevant protein antigen, as exemplified by—but not limited to—two vaccine-relevant antigens of hepatitis B virus (HBV), i.e. its envelope protein (hepatitis B surface antigen, HBsAg) and its nucleoprotein (hepatitis B core antigen, HBcAg). Accordingly, a protein antigen, whether or not intended to raise protective immune responses against itself, can be suitably and advantageously co-delivered with DNA vaccines formulated in aluminium phosphate, aluminium hydroxy-phosphate, or another suitable negatively charged mineral adjuvant.
It was further found that AlPO4 is particularly suitable as an adjuvant to formulate a vaccine composition that delivers expression plasmid-encoded antigens together with recombinant protein antigens. The vaccine composition combines (i) the adjuvant effect of AlPO4, (ii) the adjuvant effect of plasmid DNA, (iii) the enhanced immunogenic delivery of a DNA vaccine by AlPO4, and (iv) the potent co-priming of multispecific, humoral and cellular immune responses to a variety of antigens that usually is achieved only by immunization with vaccines containing live, attenuated or killed pathogens.
When analyzing the priming of murine humoral and CTL responses to vaccine-relevant antigens formulated in aluminium-based adjuvants, no boosting injections were given to exclusively determine the potency of the vaccine formulations under study in priming CTL and T cell-dependent immune responses. (It will be understood that boosting injections are not excluded from the the present invention).
It is known that plasmid DNA and proteins with an acidic IEP adsorb efficiently onto Al(OH)3, but not to AlPO4 [J. B. Ulmer et al., Vaccine (1999) 18:18-28; S. J. Seeber et al., Vaccine (1991) 9:201-2031. An immunogenic delivery of DNA vaccines with concentional Al(OH)3 adjuvant has not been achieved while Al(OH)3 is widely used to deliver protein antigens in immunogenic form. Without being bound to any theory, the present inventors believe that the high content of phosphate in the DNA strand may give lead to the very high binding affinity to Al(OH)3 and that this binding impairs the subsequent transcription of the protein antigen encoded by the DNA. A “depot effect”, i.e. the slow release of antigen in situ combined with an immune-stimulating effect of the aluminium salt, has often been proposed to underlie the adjuvant activity. The lack of adsorption of plasmid DNA onto AlPO4 (but not Al(OH)3) seems to be an essential prerequisite for its ability to deliver DNA vaccines in an adequate and functional form. When low doses of a “naked” DNA vaccine are injected i.m. without adjuvants, specific priming of CTL, but not of serum antibody responses is observed [W. Böhm et al., J. Immunol. Methods (1996) 193:29-40]. Delivery of a DNA vaccine by AlPO4 did not enhance its ability to prime specific, polarization of the response against the individual components of the vaccine. The antigens encoded by DNA vaccines would preferentially prime Th1 immunity while the antigens formulated into the AlPO4 as recombinant proteins would preferentially induce Th2 immunity. This is clearly shown in
Experimental vaccines were formulated in which low doses (1-5 μg/mouse) of antigen-encoding plasmid DNA together with low doses of either a model protein antigen (BSA), or a vaccine-relevant protein antigen (HBsAg or HBcAg) was mixed with aluminium phosphate or aluminium hydroxyde. The immune responses elicited by this combination vaccine against the protein antigen adsorbed to aluminium phosphate were enhanced in the presence of the DNA vaccine, and did not interfere with immune responses against the antigen encoded by the DNA vaccine.
To be more specific, low doses of different DNA vaccines formulated with AlPO4 induced enhanced humoral responses and supported priming of MHC class I-restricted cellular immunity. Different proteins mixed with the plasmid DNA vaccine in the AlPO4 formulation did not impair its immunogenicity. Co-injection of two different, vaccine-relevant antigens in the same AlPO4 formulation, one as a DNA vaccine, the other as a recombinant protein, elicited polyvalent, humoral and cellular immune responses to all antigens delivered. The isotype profiles of the induced humoral responses and the cytokine profiles of the specifically primed T cell responses indicated that the combined vaccines supported co-priming of Th1- and Th2-biased, as well as differentiated responses.
Therefore, protein antigen-containing mineral-based, negatively charged adjuvants, such as aluminium phosphate and for example also aluminium hydroxy-phosphate can efficiently enhance not only plasmid DNA vaccines but also provides a novel strategy for immunogenic, multivalent combined protein/DNA vaccine delivery.
The invention is further illustrated by the following examples which, however, are not intended to limit the invention in any respect.
Experimental
Immunization of BALB/c mice with protein- or DNA-based vaccines was chosen as the preclinical model since a significant amount of the preclinical experience obtained with novel vaccination approaches has been generated in this species.
Standard immunization procedures were used based on the intramuscular injection of either 5, 50 or 100 μg plasmid DNA vaccines, or 0.5-5 μg protein antigen.
1. Mixing a Model Protein (Antigen) Together with a Plasmid DNA Vaccine to Al Phosphate or Al Hydroxyphosphate Enhances the Immunogenicity of the Latter
BALB/c mice were immunized i.m. with 5, 50 or 100 μg pCl/S plasmid DNA (encoding the small HBsAg).
The DNA was delivered:
The antibody response was determined 4 weeks after a single immunization.
(A) BSA System (See Table 1)
(B) HEL System (See Table 2)
These results allow the following conclusions:
BALB/c mice were immunized i.m. with 5, 50 or 100 μg pCl/LC149 plasmid DNA (encoding a secreted, truncated variant of the HBV core antigen HBcAg).
The DNA vaccine was delivered:
HBsAg (2 μg/mouse) was delivered.
The antibody response was determined 4 weeks after a single immunization. The results are shown in Table 3 below.
These results allow the following conclusions:
The results are shown in the following graph.
HBsAg was readily detected in S/N of mixtures of antigen with aluminium phosphate (even when as low amounts as only 1 μg HBsAg was mixed with the phosphate adjuvant) while no HBsAg was detectable after incubation with aluminium
| Number | Date | Country | Kind |
|---|---|---|---|
| 02076276.1 | Mar 2002 | EP | regional |
| 0307533.4 | Feb 2003 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP03/03353 | 3/28/2003 | WO |
