Combined electroosmotic and pressure driven flow system

Abstract

Electroosmotic flow controllers that may be used in conjunction with multiple fluids and methods of fluid flow control are described. The invention uses an electroosmotically generated flow component in combination with a pressure driven flow component to modulate fluid flow. A working fluid and a second fluid that supports electroosmotic function may be used in conjunction with embodiments of the invention. Embodiments of the invention may include salt bridges for making electrical connections between a power supply and a channel filled with a porous dielectric material and a fluid.

Description

BACKGROUND

[0002] This invention pertains to the fields of fluid handling and electroosmosis. More particularly, the invention pertains to electroosmotic flow controllers.

[0003] Flow controllers are used to manage the flow of fluids through conduits. Traditionally, control of fluid volume and or fluid composition by flow controllers is accomplished by combinations of pumps and valves. In some applications, flow controllers are used to control fluid flows on the order of many milliliters or more per minute, while in other applications, the fluid flow rates are orders of magnitude smaller.

[0004] Prior art flow controllers tend to suffer from a variety of shortcomings. In high flow and in low flow applications, prior art flow controllers have difficulty in maintaining precise flow rates in the face of changing head pressures, such as those generated by pumping devices. In addition, mechanical feedback loops used to control flow rates through flow controllers often introduce additional imprecision and dead space into flow-controlled devices.

[0005] A variety of systems could benefit from fluid controllers capable of precise control of small fluid volumes. Such systems include separation systems needed for chemical analysis, microseparation techniques developed for gene sequencing, chemical microreactors, separation methods to characterize protein expression from biological materials such as gel electrophoresis, and gradient liquid chromatography followed by mass spectrometry.

[0006] The present invention addresses these and other shortcoming of the prior art by providing flow controllers that can provide precise flow rates for a variety of systems, including systems that require small fluid volumes.

SUMMARY

[0007] The present invention provides an electroosmotic flow controller capable of controlling fluid flow through a combination of electroosmotic and pressure-driven flows where control of the system is realized by varying the electrical potential applied to an electroosmotic flow device. The inventors have recognized that by adding an electroosmotic flow component to a pressure-driven flow component, one may affect rapid and accurate flow control over a wide range of flow rates. Devices embodying the invention may be made with few or no moving parts and are compatible with most solvents. They may be readily fabricated as true microscale devices. Devices embodying the present invention may be used to reliably and accurately correct for over pressurizations, pressure waves, and flow inaccuracies caused by the use of oversized pumps in microfluidic systems. Virtually any type of pressure generator, including high-pressure syringe pumps, hand pumps, or air-driven systems, can drive the devices.

[0008] The systems can be used for variety of purposes described in the aforementioned U.S. application Ser. No. 09/924,884. One application of this system is for controlling the flow of a single fluid. In another version of the flow controller, the electroosmotic device can be used for controlling the flow of a mixture of two fluids. This system includes a channel in which the two fluids flow. The channel has a fluid inlet that is in fluid communication with a first fluid source and a second fluid source, which are at pressures P1 and P2, respectively. The channel also has a fluid outlet that is in fluid communication with the fluid inlet and a fluid terminus at pressure P3, also referred to as outlet pressure. The outlet pressure is less than both P1 and P2. To control the flow of the fluid, an electroosmotic device is built into the channel by means of a porous dielectric material disposed in the channel and a pair of electrodes positioned so that the channel is electrokinetically active when a power supply applies an electrical potential to the electrodes. The electric potential generates an electroosmotically-driven flow component through the channel that modulates at least one of the pressure driven flows. To limit the pressure and flow rates at nodes or junctions in the system, flow restrictors can be provided at various locations in the system.

[0009] The system can include a control system, which includes a sensor for monitoring at least one fluid property and a feed back control mechanism operatively connected to the sensor and the power supply. The feed back control mechanism maintains at least one fluid property within a predetermined range by modulating the electrical potential applied by the power supply.

[0010] Optionally, two electroosmotic devices can be used in a system, where one serves to control the ratio of the two fluids and the second serves to control the total amount of fluid flow (FIGS. 15a and 15b).

[0011] The system can also serve to control the flow of a working fluid, such as a fluid introduced into a chromatograph (FIG. 17). Electroosmotic fluid, fluid that supports electroosmotic flow in the device, under pressure is provided with two flow paths, one to drive the working fluid, which is located in a fluid storage element or a cartridge, and the other through an electroosmotic device. By varying the potential of the electrodes of the electroosmotic device, varying amounts of the electroosmotic fluid are utilized for pumping the working fluid.

[0012] When the working fluid is displaced and the cartridge emptied, a valving system may be utilized so that a second cartridge can replace the first cartridge while the first cartridge is flushed and refilled, then after the second cartridge is emptied, it may be replaced by the first and the cycle repeated.

[0013] In another version of the invention, a fluid storage element for storing electroosmotic fluid can be placed immediately before the electrokinetically active element and forced into the electrokinetically active element by a working fluid under pressure.

BRIEF DESCRIPTION OF THE DRAWINGS

[0014] These and other features, aspects and advantages of the present invention will become better understood with regard to the following description, appended claims, and accompanying drawings where:

[0015] FIG. 1 illustrates an embodiment of the flow controller of the present invention.

[0016] FIG. 1 a illustrates a cross-section through a channel filled with a porous dielectric material.

[0017] FIG. 2 illustrates a voltage-controlled flow splitter in accordance with an embodiment of the invention.

[0018] FIG. 3 illustrates an embodiment of the invention that includes a sensor and a servo loop controller for generating feedback signals and adjusting the power supply.

[0019] FIG. 4 illustrates an embodiment of the invention that includes two sensors and a servo loop controller for generating feedback signals and adjusting the power supply.

[0020] FIG. 5 illustrates an embodiment of the invention that includes a position or displacement sensor.

[0021] FIG. 6 illustrates an embodiment of the invention used to control the flow of two fluids. This embodiment can be used for generating fluid mixtures and gradients of the fluid mixtures for use in separations technologies.

[0022] FIG. 7 illustrates controlled pressure generated by a flow controller of the invention despite varying driving pressure.

[0023] FIG. 8 illustrates controlled pressure generated by a flow controller of the invention despite decay in driving pressure.

[0024] FIG. 9 is a graph showing driving pressure and column pressure as functions of time.

[0025] FIG. 10 is a graph showing reproducibility of water:acetonitrile gradients.

[0026] FIG. 11 illustrates an embodiment of the invention that provides a method to remove the electrode from the channel and an increased range of operating conditions.

[0027] FIG. 12 illustrates a series-mode embodiment of the current invention wherein a second fluid is mixed with a working fluid to improve the performance and operating range of the electroosmotically-driven flow controller element.

[0028] FIG. 13 illustrates a shunt-mode embodiment of the current invention wherein a second fluid is mixed with a working fluid to improve the performance and operating range of the electroosmotically-driven flow controller element.

[0029] FIG. 14 illustrates an embodiment of the invention that promotes mixing of two fluids before they enter the electroosmotically-driven flow controller element.

[0030] FIG. 15 a illustrates an embodiment of the invention having two, separately powered electroosmotically-driven flow controller elements.

[0031] FIG. 15 b illustrates an embodiment of the invention having two electroosmotically driven flow controller elements that share a power source.

[0032] FIG. 16 illustrates an embodiment of the invention in which sixth and seventh flow elements are connected in series between a second fluid source and a drain.

[0033] FIG. 17 illustrates an embodiment of the invention that includes a charge of working fluid stored in one of the flow elements.

[0034] FIG. 18 illustrates a valve configuration that may be used to switch flow elements of flow controllers.

[0035] FIG. 19 illustrates an embodiment of the invention that includes a charge of a fluid stored in one of the flow elements, wherein the fluid is selected to support electroosmotic function of the electroosmotically active element.

[0036] FIG. 20 illustrates a series-mode embodiment of the invention that includes a charge of a fluid stored in one of the flow elements, wherein the fluid is selected to support electroosmotic function of the electroosmotically active element.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

[0037] The present invention utilizes principles of electroosmotic flow for fluid control purposes. Therefore, we will describe some of the basic principles of this phenomenon. Electroosmotic flow, also known as electrokinetic flow, can compete with or even dominate the flow that could be produced by application of a pressure difference across a channel. Electroosmotic flows in the present invention are generated using appropriate fluids and dielectric materials with application of an electrical field utilizing electrodes. The fluid provides a high zeta potential with respect to the porous dielectric material.

[0038] It is desirable that the magnitude of this zeta potential be in the range of about unity to 150 mV or greater. The zeta potential may be either positive or negative in sign. The sign and magnitude of the zeta potential are dependent on the dielectric constant of the fluid, the pH of the fluid, the ionic strength of the fluid and the type of ions in the fluid.

[0039] The fluid may be a pure fluid or a mixture of pure fluids that may have in addition some small concentration of a conducting species such as various ions. Preferably, the pure fluids should have high dielectric constant (between about 5 and 100 relative units), low dynamic viscosity (between about 0.1 and 2 centipoise) and low conductivity (between about 10−4 and 10−14 mho/m). Additives are preferably introduced to define or control the pH and ionic strength of the fluid. Additives should be of a kind and of a concentration to completely dissolve in the fluid. The kind and concentration of these additives preferably are chosen so as to enhance or optimize the zeta potential under the conditions imposed by the size of the pores in the porous dielectric medium.

[0040] Suitable pure fluids include by way of example, but not limitation: distilled and/or deionized water, cyclic carbonates, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 1-pentanol, 1-hexanol, 1-heptanol, benzyl-alcohol, nitromethane, nitrobenzene, butanone, dimethoxymethane, dimethylacetamide, dioxane, p-dioxane, acetonitrile, formamide, methyl formamide, tetrahydrofuran, dimethyl formamide, acetone, acetic acid, triethylamine, dichloromethane, ethylene glycol, and dimethylsulfoxide.

[0041] To yield a zeta potential, generally, the surface of the dielectric material exhibits acidic or basic sites that become ionized in the presence of the fluid. These ionizable surface sites may be native to the material or may be the result of adsorption or grafting of some species onto the surface material.

[0042] Native ionizable materials include by way of example, but not limitation: silica (acidic), alumina (amphoteric), and Nylon (zwitterionic, carboxyl and amine). The sign of the zeta potential is the same as the sign of the net surface charge.

[0043] As an example of adsorption leading to surface charge, admixtures of polyethylene or polypropylene with ionic surfactants can be used. Polyethylene and polypropylene are non-polar polymers having no native ionizable sites. In an aqueous solution containing certain ionic surfactants (e.g. sodium dodecyl sulfate), the hydrophobic tail of the surfactant adsorbs to the polymer. The charged end of the surfactant then appears as a charge site on the surface.

[0044] The degree of ionization of the surface sites depends on the pH of the fluid. In most cases there is a pH at which the surface is net neutral and hence the zeta potential is zero. The zeta potential reaches a maximum value for pH values well-above (for acidic surface sites) or pH values well below (for basic surface sites) the pH value at which the surface is net neutral. Ionizable surface sites can be added to a material by chemical reaction or grafting, or induced by creation of reactive surface chemistry or creation of defects via plasma or radiation treatment.

[0045] The dielectric material is selected for properties of: high zeta potential, sign of the zeta potential, insolubility and stability in the fluid with additives, low electrical conductivity, and sufficient mechanical strength.

[0046] Examples of suitable oxide materials include: silica, alumina, titania, zirconia, cerium oxide, lanthanum oxide, yttrium oxide, hafnium oxide, magnesium oxide, and tantalum oxide. These oxides may be amorphous or glassy or crystalline and may be combined in mixtures having other minor oxide components.

[0047] Examples of suitable glass materials include: crown or float or boro-silicate glasses, lanthanum or flint or dense flint glasses, Pyrex™.

[0048] Examples of suitable nitride materials include: silicon nitride, boron nitride, and aluminum nitride.

[0049] Examples of suitable polymers include: Nafion™ (Dupont Trade name, a sulfonated PTFE), polysulfone, polyethersulfone, cellulose acetate, mixed cellulose esters, polycarbonate, polyacrylonitrile, polyvinylidene fluoride, polyamide (Nylon), silicone elastomers, polymethacrylate, and nitro-cellulose.

[0050] Other classes of suitable materials include certain semiconductors, carbides (e.g. titanium carbide) and silicides (e.g. germanium silicide).

[0051] Counterions are ions in the fluid that have a charge sign opposite the sign of the zeta potential. Increasing the concentration of counterions in the bulk fluid tends to shield the surface charge and thus reduces the magnitude of the zeta potential. As an example, when silica is the dielectric material exposed to water at pH 7 as the pure fluid and KCl is used as an additive, the zeta potential for this system is negative with magnitudes of about: 120 mV, 100 mV, 70 mV and 30 mV for KCl concentrations of 0.1, 1, 10 and 100 millimolar, respectively. The valence of the counterion may also have a pronounced effect on the character of the zeta potential. Polyvalent (i.e. multiply charged) counterions may bind to the surface sites thus changing the pH of zero net charge (i.e. the “isoelectric point”). For example, silica in the presence of a singly valent counterion (e.g. Na+) displays an isoelectric point of about 2.8, whereas silica in the presence of a bivalent counterion (e.g. Ca2+ or Ba2+) displays an isoelectric point in the range of 6 to 7. In this regard, the transport fluid preferably is selected or purified to be substantially free of polyvalent counterions.

[0052] The ionic additives that can be added to the fluid may be broken into two general classes: those that fully ionize (e.g. salts, strong acids and strong bases) and those that partially ionize. The former class can be employed primarily to establish the ionic strength of the fluid. The latter class can be employed primarily to buffer the fluid and thus establish and maintain the pH of the fluid. The two classes often are used in conjunction. The buffering species can exist in polyvalent states (e.g. formate exists as neutral or singly charged whereas phosphate exists as neutral, singly, doubly and triply charged). Thus the choice of a buffering compound is made in view of the issue of polyvalent counterions discussed above.

[0053] Examples of ionic and buffering additives include but are not limited to: alkali-halide salts, mineral acids and bases, organic acids and bases, phosphates, borates, acetates, citrates, malates, formates, carbonates, chlorates, nitrates, sulfates and sulfites, nitrates and nitrites, ammonium-, methylarruonium-, ethylammonium-, propylammonium-salts, BIS, MES, TRIS, TES, HEPES, TEA.

[0054] Certain compounds, sometimes referred to as anti-static agents, are known to alter or eliminate the zeta potential. For example special agents are added to hydrocarbon fuels to eliminate zeta potentials and thus prevent static buildup during pumping and transport. As a further example, special agents are added to shampoos and conditioners again to eliminate the zeta potential and prevent static buildup. Certain surfactants represent one class of these agents. In this regard the fluid is selected or purified so as to be substantially free of agents that degrade or eliminate the zeta potential. As examples: addition of small quantities of the surfactant SDS (sodium dodecyl sulfate) is known to increase the zeta potential of silica in aqueous solutions. The effect of the surfactant CTAB (cetyl trimethylammonium bromide) on silica in water is to reduce the zeta potential upon addition at low concentrations, to a value near zero as the concentration is increased, and to reverse the sign of the zeta potential at even higher concentrations. Addition of polyamines is also known to reduce or reverse the zeta potential of silica. Surface modification properties of surfactants are reviewed by M. J. Rosen, ‘Adsorption of surface-active agents at interfaces: the electrical double layer,’ Chapter II in, Surfactants and Interaction Phenomena (Wiley, N.Y., 1986), pp. 33-107.

[0055] The region of net charge in the fluid and adjacent to the dielectric surface extends some distance into the fluid. The one-on-e (1/e) thickness of this layer is approximately the Debye length in the bulk fluid. The Debye length at a temperature of 20° C. has a value of about 0.034 nm times the square root of the ratio of the fluid dielectric constant to the fluid ionic strength (the later taken in units of mols/liter). For one millimolar KCl in water the Debye length is about 9.6 nm.

[0056] Pores in the porous dielectric material vary in size along the length, and a variety of pore sizes may be present. Thus the dielectric material, saturated with a fluid at some given ionic strength, may have some subset of pores that contain substantially overlapped regions of net charge (here termed ‘nanopores’) with the balance of the pores containing some amount of core fluid that is free of charge-layer overlap (here termed ‘regular’ pores). All of the pores will transport current and hence ionic species, but the nanopores transport flow at a greatly reduced rate compared to the regular pores. It is desirable to apply a current so as to create a flow with minimal alteration of fluid ionic composition. The presence of nanopores reduces the efficiency of this process and may also lead to substantial and performance-degrading ionic strength, composition, and pH gradients across the porous element.

[0057] The porous dielectric materials may be fabricated by a wide variety of methods, examples include but are not limited to the following:

[0058] Packed particles where the particles may be glass or ceramic or polymers. The particles may be held in place (i.e. confined in the channel) by any method known in the art, including but not limited to end-frits or other mechanical restrictions, or by cold welding under pressure or chemical bonding.

[0059] Synthetic porous opaline materials, such as those described in, for example, A. P. Philipse, ‘Solid opaline packings of colloidal silica spheres,’ J. Mat. Sci. Lett. 8 pp. 1371-1373 (1989), and porous materials created by using opalines as a template, as described in, for example, J. E. G. J. Wijnhoven and W. L. Vos, ‘Preparation of photonic crystals made of air spheres in titania,’ Science 281 pp. 802-804 (1998).

[0060] Phase separation and chemical leaching of a glass, for example the Vycor process as applied to a borosilicate or other composite glass as described in, for example, T. Yazawa, ‘Present status and future potential of preparation of porous glass and its application,’ Key Engineering Materials,’ 115 pp. 125-146 (1996).

[0061] Solgel or aerogel process in silica, alumina, titania, zirconia and other inorganic-oxides or mixtures thereof.

[0062] Zeolite and zeolite-like porous media as described in, for example, Y. Ma, W. Tong, H. Zhou, S. L. Suib, ‘A review of zeolite-like porous materials,’ Microporous and Mesoporous Materials 37 pp. 243-252 (2000).

[0063] Phase separation of polymer—inorganic oxide solutions as carried out using, for example the SilicaRod process described in, for example, K. Nakanishi and N. Soga, ‘Phase separation in silica sol-gel system containing polyacrylic acid I. Gel formation behavior and effect of solvent composition,’ J. Non-crystalline Solids 139 pp. 1-13 (1992).

[0064] Direct machining by lithography and etching, molding, casting, laser ablation and other methods known in the arts. Direct machining may be used to generate, e.g., regular or irregular arrays of microchannels or pillars fabricated from a material that, in combination with a desired pumping of transport liquid, gives rise to a zeta potential. Such microchannels or pillars may be used as the porous dielectric materials of embodiments of the present invention.

[0065] Porous polymers as prepared by film stretching, sintering, track etching, casting followed by leaching or evaporation, slip casting, phase inversion, thermal phase inversion. Like methods are often employed in the manufacture of polymer filter membranes.

[0066] Porous polymer monoliths as described in, for example, E. C. Peters, M. Petro, F. Svec and J. M. Frechet, ‘Molded rigid polymer monoliths as separation media for capillary electrochromatography,’ Anal. Chem. 69 pp. 3646-3649 (1997).

[0067] Anodic etching as applied to silicon, as described in, for example, J. Drott, K. Lindstrom, L. Rosengren and T. Laurell, ‘Porous silicon as the carrier matrix in micro structured enzyme reactors yielding high enzyme activities,’ J. Micromech. Microeng. 7 pp 14-23 (1997) or as applied to aluminum as described in, for example, O. Jessensky, F. Muller and U. Gosele, ‘Self-organized formation of hexagonal pore structure in anodic alumina,’ J. Electrochem. Soc. 145 pp. 3735-3740 (1998).

[0068] The porous materials may be fabricated in-channel or may be fabricated, possibly machined or cut, and then inserted or sealed into the channel. The surface properties may be altered before or after placement within a channel.

[0069] The sign and magnitude of the zeta potential can be altered or enhanced by modification of the surface or bulk chemistry of the porous material as described above. Modification of surface chemistry is generally done by reaction with sites (e.g. silanol, hydroxyl, amine) that are present on the native material. Modification of the bulk chemistry is generally done by synthesis of a material that directly incorporates ionizable sites. Examples include but are not limited to the following:

[0070] Modification of the bulk chemistry of a polysulfone or polyethersulfone to convert some portion of the S═O groups to sulfonic acids.

[0071] Modification of the bulk chemistry of PTFE to attach side chains terminated in sulfonic acid groups (Dupont product Nafion).

[0072] Modification of the bulk chemistry of a polyethersulfone or a polyvinyledene fluoride to introduce quaternary amines.

[0073] Modification of the bulk or surface chemistry of a polyamide (Nylon) to provide a material with only carboxy (acidic) or amine (basic) surface sites.

[0074] Modification of a zwitterionic material (e.g. Nylon) to terminate one of the existing ionizable sites with a nonionizable end group. The material is then converted to one having only a basic or an acidic site, rather than one having both types.

[0075] Activation of a polymer material by introduction of defects or creation of cross-links via exposure to a plasma, ultraviolet or ionizing radiation.

[0076] Modification of surface silanol groups with methoxy- or chloro-silanes to create amino groups or sulfonic acid groups.

[0077] The principles and operation of the invention will now be described by reference to the following figures that are intended to serve as illustrative embodiments but not to limit the scope of the invention.

[0078] FIG. 1 illustrates an “in-line” or “series type” flow controller embodiment of the invention. With respect to FIGS. 1 and 1a, a channel 100 of total cross-section A and of total length L is packed with a porous dielectric medium 104. The channel 100 has an inlet 101 that is in fluid communication with a fluid source 102 at pressure P1 and an outlet 103 at pressure P2, where P2<P1. Throughout this description, we have assumed negligible resistance to fluid flow (and so negligible pressure drops) between the fluid source 102 and the inlet 101, and between the fluid outlet 103 and the fluid-collection reservoir 109. Under such circumstances, the pressure drop ΔP across the channel 100 is equal to P2−P1. One of skill in the art readily will appreciate how to modify the equations below to account for pressure drops between the fluid source 102 and the inlet 101, and between the fluid outlet 103, and the fluid collection reservoir 109 by adjusting the term ΔP so that it accurately reflects the pressure drop across the channel 100. The flow rate Q is produced by the combined action of a potential difference ΔV generated by power source 107, and applied to the fluid within the channel through spaced electrodes 105, 106, and a pressure difference ΔP between the channel inlet 101 and the channel outlet 103.

[0079] With reference to FIG. 1, the porous dielectric material 104 is contained in a fluid-impermeable ‘channel’ 100. Channel materials are selected to meet requirements for mechanical strength, dielectric breakdown strength, transport fluid and fluid additive compatibility, and the capacity to retain the porous dielectric material 104. The geometry of the channel 100 covers the entire range from long in length and small cross section to short in length and large cross section. An example of the former geometry is a channel 100 that may be a capillary tube or a covered microchannel formed in a substrate having cross sectional shapes including round to rectangular to rectangular with sloped or curved sides. This channel 100 may be formed by any of the means known in the art. An example of the latter geometry is a large diameter and thin porous membrane.

[0080] The choice of pore size, topology numbers and physical geometry (e.g. porous element thickness and cross-sectional area) are particular to a given application. This then drives the needs for ionic strength and buffering capacity. In general, the following considerations may be taken into account for practicing preferred embodiments of the present invention.

[0081] Use of singly valent counterions for a well-defined hence well-behaved zeta potential.

[0082] Absence of compounds in the fluid that degrade or eliminate the zeta potential.

[0083] Use of the lowest concentration of ionic species compatible with ‘minimal’ double layer overlap (i.e. a concentration yielding a fluid Debye length that is less than about one-fifth the characteristic pore size).

[0084] Use of the lowest concentration of buffering ionic species consistent with establishing and maintaining the pH of the fluid.

[0085] Use of ionic species that are compatible with, well soluble, and well dissociated in the fluid.

[0086] A pore size distribution that is preferably monodisperse and if polydisperse does not contain occasional large pores or defects (e.g. cracks or voids) and contains no or a minimal number of ‘nanopores’

[0087] Use of a porous dielectric material 104 that is less conducting than the fluid with additives.

[0088] Use of a porous dielectric material 104 with a dielectric strength sufficient to withstand the potentials applied without dielectric breakdown.

[0089] Use of a porous dielectric material 104 that is mechanically strong enough to withstand the pressures applied both as regards the ability to withstand compression and collapse, and the ability to remain attached to the material of the bounding channel.

[0090] Use of a porous dielectric material 104 that is resistant and insoluble in the transport fluid with additives.

[0091] Use of a channel material that is an insulator, and in particular the channel material should be less conducting than the fluid with additives.

[0092] Use of a channel material with a dielectric strength sufficient to withstand the potentials applied without dielectric breakdown.

[0093] Use of a channel material that is mechanically strong enough and thick enough to withstand the pressures applied.

[0094] Use of a channel material that is resistant and insoluble in the transport fluid with additives.

[0095] Use of a fluid with a high value of the dielectric constant and a low value of the dynamic viscosity.

[0096] Use of a combination of fluid, surface chemistry and additive ionic species chemistry that provides a high value of the zeta potential.

[0097] Use of a fluid that is a pure fluid or a highly miscible mixture of pure fluids.

[0098] It is well-known to one of skill in the art that application of an electrical potential to a fluid via electrodes 105, 106 in that fluid can generate a current through the fluid, and that gas will be generated at the electrodes 105, 106 via electrolysis of the fluid. It is further appreciated that gas generation within a closed fluid channel may be undesirable. Thus, as shown in FIG. 1, a bridge 108 may be used to connect the electrodes 105, 106 in the fluid-filled reservoirs 102, 109 to the fluid in the channel 100. Such bridges are well-known in the art, and are described, for example, in C. Desiderio, S. Fanali and P. Bocek, ‘A new electrode chamber for stable performance in capillary electrophoresis,’ Electrophoresis 20, 525-528 (1999), and generally comprise a porous membrane or porous solid selected to have sufficiently small pores so as to minimize fluid flow through the bridge, while at the same time to provide for the transport of ions (i.e. to allow current flow). Typical bridge materials include Nafion™ (an ion-selective polymeric membrane) or porous Vycor™ (a phase-separated and etched porous glass having a pore size on the order of 5 nm).

[0099] The flow rate in the channel 100 may be written as: Q=(νΔV−κΔP)A/LF. This relation is a well-known combination of Darcy's law for pressure driven flow and the Helmholtz-Smoluchowski relation as adapted for electroosmotic flow in porous media. Here ν is the effective electroosmotic mobility, κ is the Darcy permeability of the porous media multiplied by F and divided by the dynamic viscosity of the liquid, and F is the formation factor of the porous media and is simply greater than or equal to the inverse of the connected porosity. F is by definition unity for a channel that does not contain porous media and takes values greater than unity for a channel containing porous media. The formation factor may be related to more common descriptors of porous media via F=τ2/φ where τ is termed the tortuoisty and φ is the connected porosity of the solid. The connected porosity is the wetted volume fraction that represents the through-connected pores and excludes dead-ended pores. Each of these descriptors may be determined using any of the methods well known in the art.

[0100] The Debye length scale can be altered by changing the ionic strength of the fluid and is preferably less than about one-fifth the characteristic pore size of the porous dielectric medium 104. For Debye lengths greater than about one-fifth the characteristic pore size, the charged layers on opposing walls of the pore begin to substantially merge having the effect of reducing the apparent zeta potential. For quantitative determination of the degree of double layer overlap the characteristic pore size, Dpore, is preferably taken as defined by D. L. Johnson and P. N. Sen, Phys. Rev. B 37, 3502-3510 (1988); D. L. Johnson, J. Koplick and J. M. Schwartz, Phys. Rev. Lett. 57, 2564-2567 (1986); and D. L. Johnson, J. Koplick and R. Dashen, J. Fluid Mech. 176, 379-392 (1987). This definition of Dpore produces a strong weighting in favor of the larger through-pores in a porous medium.

[0101] Using the definition of Dpore given above, the Darcy permeability is given by:

kD=Dpore2M/F

[0102] where M is termed the ‘pore geometry number’, which equals {fraction (1/32)} for a circular tube and approximately equals {fraction (1/32)} for tubes of other cross sectional shapes and many porous media.

[0103] The effect of charge-layer overlap in simple geometries (e.g. slit or circular pores) has been studied theoretically. See, e.g., C. L. Rice and R. Whitehead, ‘Electrokinetic flow in a narrow cylindrical pore,’ J. Phys. Chem. 69 pp. 4017-4024 (1965); and D. Burgreen and F. R. Nakache ‘Electrokinetic flow in ultrafine capillary slit,’ J. Phys. Chem. 68 pp. 1084-1091 (1964). The conclusions of these studies can be applied analogously to a general porous medium through the use of Dpore as defined above.

[0104] The effective electroosmotic mobility may be written as: ν=εζ(1−ξ)/μ where ε and μ are the dielectric permittivity and dynamic viscosity of the fluid, respectively, ζ is the zeta potential and ξ is a factor that provides for the effect of overlapping net charge layers (i.e. a reduction of the apparent zeta potential under conditions that the thickness of the charge layers becomes on the order of the size of the pores in the media). The zeta potential, hence the electroosmotic mobility, may be signed positive or negative depending on the nature of the fluid and the dielectric material (e.g. for a porous dielectric material 104 composed of TiO2 saturated with an aqueous solution, the zeta potential will have a positive sign at low pH and a negative sign at high pH and will be negligibly small at the material iso-electric point which for TiO2 is at about pH 6.2).

[0105] The electrokinetic property of an electrokinetically active element is characterized by α=νΔV/κP1 where ΔV is the voltage applied across the element. The quantity α is dimensionless and may be thought of as the electroosmotic flowrate produced by the potential ΔV divided by the pressure-driven flowrate produced by a pressure difference equal to P1. A useful metric for the performance of an electrokinetically active material is the quantity ν/κ, which has units of psi/volt. Using these definitions, the flowrates through elements may be appropriately summed at junctions and then solved for the pressures at the junctions.

[0106] The present invention employs a combination of pressure- and electroosmotically-driven flows in a channel 100 filled with a porous dielectric material 104. The applied potential preferably is selected to yield an electroosmotic flow in the same direction as the pressure-driven flow (e.g. for TiO2 at high pH, hence a negative zeta potential hence a negative electroosmotic mobility, the potential would be applied with the negative terminal downstream with respect to direction of the pressure-driven flow). In this configuration the maximum flow rate through the channel 100 will be given by the flow rate equation above and only limited by the magnitude of the potential applied, whereas the minimum flow rate will be for purely pressure-driven flow that is with ΔV=0, hence Q=−κΔP A/LF. Thus the combination of pressure- and electroosmotically-driven flow in the channel 100 filled with the porous dielectric material 104 provides a voltage-controlled means to vary the flow rate through that channel. In effect, flow control is provided by varying the degree of electroosmotic ‘assist’ to the pressure-driven flow through the channel. As is explained in greater detail with respect to other preferred embodiments described below, sensors may be used to monitor parameters such as pressure, flow rate, etc. at one or more points in the flow controller system. Signals arising from these sensors may be used in a servo loop to maintain the signal within a predetermined range by adjusting the voltage outputted by the power supply in response to deviations between the signal and a predetermined set point.

[0107] The system of FIG. 2 illustrates another preferred embodiment of the invention resulting in a device that acts as a voltage-controlled flow splitter. Fluid is supplied from a source 102 at a gauge pressure P1 and subsequently split at a node 202 to flow through the device to a pair of fluid outlets 103, 204 at gauge pressures P2 and P3, respectively. Both P2 and P3 are less than P1. The system of FIG. 2 may include a first flow resistor also referred to as a flow element 205 with an inlet 206 that is in fluid communication with the fluid source 102 at pressure P1 and an outlet 207 in fluid communication with the node 202 at pressure Pnode. The first flow element 205 can be included to provide a pressure-driven flow resistance, or Darcy flow resistance, between the fluid source 102 at pressure P1 and the node 202 so as to reduce the flow rate and pressure available at Pnode such that the maximum available pressure and maximum available flow rate established at the node 202 is compatible with the electroosmotic flow rate of the channel 100. This is accomplished by making the resistance of the first flow element 205 to be some fraction or multiple of the flow resistances of the channel 100 and a third flow element 201 having an inlet 203 and an outlet 204.

[0108] The gauge pressure P2 can be zero, that is, ambient pressure. However, this embodiment is not limited to this condition, which is provided purely for illustration of this application. The flow rate Q3 through the third flow element 201, when P2=0 is given by:

Q 3 =k 3 (k1P1(1−y)−(k1+k2)P3)/(k1+k2+k3)

[0109] If k≡κ A/LF and y≡(ν2/κ2)k2ΔV/k1P1. The variable k can be considered effectively as the above-mentioned pressure-driven flow resistance parameter or conductance for each flow element or channel where A is the effective cross section area and L is the length of the element or channel. Thus for ΔV=0, hence y=0, the flow rate through the third flow element 201 has a value of:

[0110] ti Q3=k3(k1P1−(k1+k2)P3)/(κ1+k2+κ3)

[0111] whereas this flow rate Q3 (i.e. the flow rate through the third flow element 201) is zero when:

y= 1−(k1+k2)P3/k1P1

[0112] hence this flow rate is zero when the potential is set to a value of

ΔV=(k1P1−(k1+k2)P3)κ2/V2)

[0113] The flow rate Q3 through the third flow element 201 can be made negative (i.e. the flow direction through the third flow element reversed) by the application of even higher values of the potential.

[0114] The Darcy flow resistance for the first flow element 205 is selected based upon on the desired range of flow rates through the third flow element 201 and the electroosmotic flow rate that is achieved when a maximum voltage is supplied across the channel 100 by the power source 107. For example, if one desires the ability to halt flow through the third flow element 201, Pnode must be equal to P3. The pressure at the node 202 is given by: Pnode=(k1P1(1−y)+k3P3)/(k1+k2+k3). Thus, the relative resistances of the first flow element 205 and the channel 100 should be designed to allow electroosmotic flow through the channel 100 to be equal to the pressure driven flow through the first flow element 205. Appropriate selections of relative flow resistances for the channel 100, the first flow element 205, and the third flow element 201 for a particular application are readily determined using the equations provided above by those skilled in the art.

[0115] FIG. 3 illustrates an embodiment similar to that illustrated in FIG. 2, except for the addition of a first sensor 301 to monitor the pressure at the common node 202 of the flow elements shown in FIGS. 2 and 3. The first sensor 301 can be employed along with a servo loop controller 302 as part of a sense-and-control loop to regulate the pressure at the common node 202 and hence the flow rate, Q3, through flow element 201. The flow rate Q3 through the third flow element 201 also may be monitored directly or indirectly through a second sensor 311 as described in greater detail below. Such regulation may be desirable to compensate for variations in source pressure P1 (resulting, for example, from fluctuations in the output of a pump providing the pressure P1). Again referring to the example in which the gauge pressure P2 is zero (and again not limiting the invention to this particular condition), the flow rate Q3 through the third flow element 201 is given by Q3=k3(Pnode−P3) where the pressure at the node 202 is given by: Pnode=(k1P1(1−y)+k3P3)/(k1+k2+k3). Thus variations in P1 can be compensated by adjustments to ΔV, hence y, so as to maintain a constant pressure at the node 202 and hence a particular flow rate Q3 through the third flow element 201.

[0116] The control so achievable is limited by the condition that the pressure at P1 remains sufficiently high to supply the required flow rate. This type of feedback control may be accomplished by any of the means that are well-known in the art, for example: observing a pressure or flow reading at the node 202 by use of the first sensor 301 and manually adjusting the potential applied by the power source 107; measuring the pressure or flow at the node 202 with the first sensor 301 and supplying this measurement to an analog electronic (or mechanical) servo loop controller 302 driving an electronically (or mechanically) adjustable power supply 107; measuring the pressure or flow at the node 202 with a first sensor 301 connected to a computer and using the computer to adjust the power supply 107, optionally, with higher order corrections applied (e.g. corrections for fluid or sensor temperature variations) in light of other data being supplied to the computer.

[0117] Multiple devices such as those illustrated in FIGS. 2 and 3, with or without servo-loop control, may be run in parallel to deliver multiple parallel sources of variable flow rate from one common source of fluid 102. The outlets of these parallel implementations need not but may terminate in loads at the same pressures. Similarly, the flow resistances and mobility coefficients of these parallel devices need not but may be the same.

[0118] The servo loop described above may employ a variety of control inputs and action outputs. By way of example, but not limitation, with the object of providing a constant flow rate Q3 through the third flow element 201 the input to the servo loop is taken as, e.g., the differential pressure across the third flow element 201 (see FIG. 4, where the first and second sensors 301 and 311 may be used to measure pressure) or the differential pressure across some other passive pressure drop arranged in series with the third flow element 201. This differential pressure then provides a measure of the flow rate via Darcy's law. Alternatively, the flow rate may be detected by other means know in the art, such as but not limited to: a turbine flowmeter, a thermal convection flowmeter, a Doppler flowmeter measured at or beyond the fluid outlet 204 of the third flow element 201.

[0119] With the object to supply a flow rate of liquid used for heat transfer and by this the control of a temperature or heat flux as a result of the flow of liquid through the third flow element 201, the first and second sensors 301 and 311 (as shown in FIG. 4) may be used to measure temperature and the third flow element 201 is taken to be one side of a liquid heat exchanger or some further downstream element. For control of temperature the input to the servo loop may be a thermocouple or thermistor or RTD or other devices known in the art. For control of heat flux the input to the servo loop may be from a heat flux sensor or the temperature change of the fluid or other means known in the art.

[0120] With the object of applying a mechanical force or displacement through the application of fluid pressure to a bellows 501 (see FIG. 5) or a piston or diaphragm or other means known in the art, the first sensor 301 may be used to generate a signal for input to the servo loop from a load cell (for force) or a displacement sensor as known in the art. One of skill in the art readily will appreciate that hydraulic mechanical systems are preferably applied under compressive load conditions. For the case where the load is naturally compressive (e.g. gravitationally or spring return loaded) a single flow control system may be used to apply and control the hydraulic force acting against the load. For this case the potential applied by the power supply 107 across the channel 100 is reduced to increase flow towards the load thus pushing against the load, whereas the potential across the channel 100 is increased to increase flow of fluid from the hydraulic actuator when the load is being returned. For the case where the load is neutral or where an active return force is required, two such flow control/servo systems may be used in a push-pull configuration.

[0121] The designs represented in FIGS. 1 through 5 illustrate several embodiments of the invention. It will be appreciated by those of skill in the art that these embodiments may be combined in a variety of series and parallel arrangements dictated by the problem or application at hand. In this regard, the embodiment illustrated in FIG. 1 may be considered as a form of in-line or series flow controller and the embodiments illustrated in FIGS. 2 through 5 may be considered forms of shunt or bleed flow controllers.

[0122] The system illustrated in FIG. 6 shows a further embodiment of the invention useful for metering two fluids into a common stream. As one possible application and to illustrate this embodiment, such a system could be used to perform controlled mixing of two reagents or buffers to be used for gradient-type high-pressure liquid chromatography (HPLC). As described above, the use of pressure sensing and servo-feedback control may be applied (as shown in FIG. 6) to monitor and/or control and/or regulate both the mixture and the output flow rate. Again this system and the invention are not limited to this particular example.

[0123] In the example of FIG. 6 sources of two fluids, A, and B, 102, 602, at gauge pressures PA and PB, are fed to two shunt-type controllers (having flow elements 100, 205, 201, 600, 608 and 625 that have inlets 101, 206, 203, 601, 626 and 609 and outlets 103, 207, 204, 603, 627 and 611, respectively, bridges 108 and 628, nodes 202, 610, and 612 and sensors 301, 614, monitoring node pressures P2A and P2B respectively) that feed fluid to a common junction 612 (at gauge pressure P3 monitored by the sensor 613) where the fluids mix. This mixture is further supplied to sample injector 616 and then to a pressure-driven chromatographic column 617. For purposes of this illustration, the outlet pressure of the chromatography column 617 and of collection reservoirs 109, 629 for the second and fourth elements 100 and 600 are taken as ambient (however the invention is not restricted to these outlet pressures, nor by these outlet pressures being the same).

[0124] The objective in this version of the invention is to provide constant flow rate to the column 617 while providing a programmed variation in fluid composition. The flow rates of fluids A and B from their respective sources 102 and 602 are independently measured and servo-controlled by two sense-and-control loops involving the first, second and third sensors 301, 613 and 614, the first and second servo loop controllers 302 and 615, the first and second power sources 107 and 607, and set-point inputs 618, 619. The programmed variation in fluid composition may be in the form of a series of step changes, or in the form of a continuous ramp (i.e. a gradient) or any of the other forms known in the separation arts. In applications requiring more than two sources of fluid, attendant flow controllers and servo loops may be combined to provide for more complicated or broad ranging fluid composition variations. Such configurations can be run in parallel from common sources of fluids to be able to perform multiple separations in parallel.

[0125] For the purpose of this illustration, sample injection through a sample loop 621 connected to a sample injector valve 616 at the head of the separation column 617 is taken to be performed by any of the means known in the HPLC arts (e.g. by a specialized sample injection valve e.g. 616, or by electroosmotic/electrophoretic injection through a porous media). For the purpose of this illustration, the end-use of the separation is taken to be any of the end-uses known in the HPLC arts (e.g. such as analyte detection by a detector 620 that measures, e.g., laser-induced fluorescence, optical absorption, refractive index or electrochemical potential; collection of the separation components; input to a mass spectrometer or ICP or NMR spectrometers; input to a next stage of separation by HPLC or LC or electrochromatographies; or preparative HPLC).

[0126] FIGS. 7 and 8 show examples of flow control using the shunt-type flow controller configuration shown in FIG. 3. The flow elements were constructed from a section of 150 micron inner diameter silica capillary packed with 0.6 mm diameter non-porous silica beads. The flow elements, pressure transducers and pressure source were connected using conventional miniature HPLC fittings.

[0127] The data shown in FIG. 7 were generated using a commercial “lead-screw” type syringe pump as the pressure source (the ripples on the driving pressure curve (line 700) correspond to the well-known pressure fluctuations produced by a syringe pump). A time t=0 the controller was switched on with a set point of ca. 225 psi. By t>2.5 minutes the set point was achieved as illustrated by a controlled pressure trace 701. Over the remainder of the test the feed rate of the syringe pump was changed several times, resulting in changes in the driving pressure 700 but the changes in the driving pressure 700 produced less than 2% variation in the controlled pressure 701. The driving pressure, oscillations apparent in the trace 700 were effectively removed by the flow controller, and so are absent in the controlled pressure trace 701.

[0128] The data shown in FIG. 8 also were generated using a commercial “lead-screw” type syringe pump. Again at t=0 the controller was switched on and the controlled pressure 801 set point of ca. 225 psi was quickly achieved. In this example the driving pressure 800 was increased and the syringe pump then was switched off resulting in decay of the driving pressure 800 over a period of time. By ca. 190 minutes the driving pressure 800 had fallen to ca. 240 psi. whereas the controller maintained the controlled pressure set point of ca. 225 psi. Thus, flow control was achieved with a driving pressure only slightly greater than the set point pressure. The top trace 802 in FIG. 8 shows the current drawn through the flow control element.

[0129] FIG. 9 shows pressure data from a nanobore capillary system driven by a traditional HPLC pump. The flow rate of the HPLC pump is monitored by a trace 900 showing that the pump output pressure is unstable in the microsystem causing 150 psi spikes. The output of a pressure transducer at the column head is shown by trace 901. Switching on the flow controller (between approximately 60 and 120 minutes) allows the pressure and flow rate to the column to be precisely controlled. Over the range where the flow controller operates the root mean squared (“RMS”) variation in pressure around the 650 psi set point is 1.7 psi. At the 8.5 nL/sec flow rate in the column, this correlates to a RMS variation in flow rate of 0.02 nL/sec.

[0130] Since the set point of the flow controller can be changed to almost any value less than the driving pressure, two or more flow controllers may be combined to deliver fast, accurate, and reproducible gradients for use in microscale separations. A single pressure source can be used to drive all of the different fluids used in the gradient. Since the flow controller is a microscale device, it is compatible with being operated in a multiple parallel configuration.

[0131] FIG. 10 shows the performance of a dual flow controller system such as the system illustrated in FIG. 6, programmed to generate water/acetonitrile gradients, illustrated in traces 1000 and 1001. The traces 1000 and 1001 correspond to pressures measured at the nodes 202 and 610 illustrated in FIG. 6. Six gradients are repeated in the figure, starting at approximately 3, 12, 21, 30 and 39 minutes. The water and acetonitrile are both sloped several hundred psi from their starting to ending pressures over the 3-minute gradient and sent to a mixing tee at the head of the nanobore separation column. The gradient changes the composition of the mixed fluid while controlling the rate at which fluid is delivered to the nanobore separation column. The starting conditions of the gradient can be reestablished in less than 1 minute. In this system a simple hand-operated pump provides the driving pressure. The majority of the flow goes directly into the HPLC column; very little waste is produced. The flow rate in the separation column is compatible with feeding directly into a mass spectrometer. This demonstrates the ability of a dual flow controller system to quickly and reproducibly generate fast gradients in a nano bore HPLC system.

[0132] As noted above, the presence of a current-carrying electrode in a closed channel may produce undesirable side effects. Bridges provide one method of removing the electrode from the channel while still providing current. As also noted above, the zeta potential is a function of fluid composition and pH. As such, any given flow control porous element may operate under some limited range of fluid conditions. FIG. 11 shows an embodiment of the invention that provides both a method of removing the electrode from the closed channel and increasing the range of operating conditions.

[0133] In FIG. 11, the second flow control element channel 100 is replaced by two such channels in parallel 100 and 1100. The fluid outlets 103, 1103 of the two channels 100, 1100 are led into separate fluid reservoirs 109, 1109, both at the terminal pressure P2 that is less than source 102 pressure P1. Power from the power supply 107 is supplied via electrodes 105, 106 in the reservoirs. Current is then carried from the power supply 107 through one channel (e.g. 100), back through the other channel (e.g. 1100) to the power supply 107. The common fluid connection of the channels, the node 202, may then be held at an arbitrary potential (preferably but not necessarily system ground). The channels 100 and 1100 comprise different zeta potential porous dielectric materials 104, 1104 having pore sizes sufficiently large to support electroosmotic flow. Note that this configuration reduces to the case of a bridge in the limit that one of the channels contains material having a pore size too small to support electroosmotic flow but large enough to still carry a current.

[0134] For example, the material 104 in channel 100 may be silica with a nominal iso-electric point of pH 3 and the material 1104 in channel 1100 may be alumina with an iso-electric point of pH 9.2. As a further example, the material 104 may be modified to display a sulfonic acid group (nominal iso-electric point of pH 1.5) and the media 1104 may be modified to support a quaternary amine (nominal iso-electric point higher than pH 14). For a fluid with a pH between the iso-electric points of the two materials the electroosmotic flow through one channel will be towards the supply anode and the electroosmotic flow through the other channel will be towards the supply cathode. This then provides flow hence flow control over a wider range of pH conditions than could be supported using a single channel and at the same time removes the current-carrying electrodes 105, 106 from the closed channels 100, 1100.

[0135] As a specific example consider the materials 104, 1104 in channels 100 and 1100 to be silica and alumina, respectively. With fluid having pH 3 channel 100 filled with silica has a negligible zeta potential and thus does not provide electroosmotic flow, but still carries current. Channel 1100 filled with alumina has a high positive zeta potential with fluid having pH 3 and thus provides the electroosmotic flow (from the common junction 202 of the channels towards the supply anode) needed for flow control. With fluid having pH 9 the roles are reversed, the silica displays a high negative zeta potential whereas the alumina has a negligible zeta potential, thus the electroosmotic flow is through the channel 100 filled with silica, from the common junction 202 of the channels towards the supply cathode. For a fluid having a pH between 3 and 9, the channels 100 and 1100 both supply some degree of electroosmotic flow and thus contribute to the ability to achieve flow control.

[0136] It is apparent that the use of any given material as the active element in the embodiments of the invention described thus far restricts the range of liquids that may be used. For example, chromatography of many proteins and small molecules is performed under acidic fluid conditions. However, silica is not viable under acidic conditions. Hence, the embodiments of the invention thus far described may require a change in the electrokinetically active material to operate in different pH ranges.

[0137] In any case, the dynamic range of the flow controller is increased by increasing the zeta potential and decreasing the square of the effective pore size of the active element. The dynamic range of the embodiments thus far described may be not as great as desired because of the need to use a material that is compatible with a particular fluid.

[0138] The following embodiments may be used in conjunction with a much larger range of liquids. A primary application of the following embodiments is thus to chromatography where the working fluid is dictated by the type of separation.

[0139] The embodiment illustrated in FIG. 12 can be used in conjunction with a working fluid that does not, by itself, support electrokinetic activity. A working fluid 1203 from a source 102 at pressure P1 flows through the third flow element 201 to a junction 202 with the first and second flow elements 205 and 100, respectively. A second fluid 1204 from a second fluid source 1201 at pressure P1A flows through the first flow element 205 also to the junction 202. The second flow element 100 is electrokinetically active (i.e., the element exhibits a zeta potential and an external potential is applied to the element) and carries the mixture of the two fluids 1203 and 1204, respectively to a terminus 1205 at pressure P2 that is less than P1 and P1A. The configuration shown in FIG. 12 may be termed a series-mode configuration.

[0140] The second fluid 1204, supplied at pressure P1A, is not necessarily the same as the working fluid 1203. Rather the second fluid 1204 is intended to be mixed with the working fluid 1203 to alter the pH or ionic strength or fluid composition and thus provide for proper operation of the electrokinetically active second flow element 100.

[0141] If we again measure the pressures with respect to the P2 gauge, the flowrate through the second element 100 is Q2=αk2P1+k2P′, where P′ is the pressure at the junction 202, and the sign of α is arranged such that the electroosmotic flow is in the same direction as the pressure-driven flow through the second element. The sign of α is made positive by selecting the sign of the applied potential and the sign of the zeta potential such that the product is positive.

[0142] The flowrate through the third element is given by 1$Q_3=P_1{k}_3\frac{k_1+k_2-{\mathrm{xk}}_1+{\mathrm{ak}}_2}{k_1+k_2+k_3}$$\mathrm{where}x=P_{1A}/P_1.$

[0143] A set of conditions may be imposed to guide the selection of element conductances. Two conditions that may be imposed are setting 1+k2/k1>x and 1+k2/k3>1/x to maintain both Q1 and Q3 positive for all positive values of α. Further conditions may be derived by requirements, if any, for the range of flowrates through the third element; the minimum being at no applied potential hence α=0, and the maximum can be a junction pressure of zero, hence Q3max=k3P1. When the junction pressure is zero, α=(k3+xk2)/k1.

[0144] A further condition may be derived by requiring flowrates through the first and third elements that yield a mixture having properties suitable for high performance electrokinetics in the second element. The mixture of the two fluids may be characterized by the ratio of flowrates through the first and third elements, Q13=Q1/Q3, given by: 2$Q_{13}=\frac{k_1{\mathrm{xk}}_2+{\mathrm{xk}}_3-k_3+{\mathrm{ak}}_2}{k_3{k}_1+k_2-{\mathrm{xk}}_1+{\mathrm{ak}}_2}$

[0145] The selection and optimization of other sets of flow element parameters, given other design conditions, becomes obvious given the fundamental relationships and examples provided throughout this document.

[0146] The following example is for illustration purposes only and is not to be taken as a limitation of the invention. The working fluid can be aqueous 10 mM trifluoroacetic acid “TFA” yielding a pH of about 2.5. In this example, the second element employs silica as the active material, specifically a packing of nominal 0.6 micron non-porous silica beads yielding a performance of over 5 psi per volt under neutral to basic pH conditions. Silica displays little or no zeta potential at a pH of about 2.5. The second fluid is a mixture of 100 mM aqueous imidazole, a weak base with a pH of about 7.15, and 1 mM HCIl The HCl is not mandatory but is added to guarantee operation of the second element even running the pure second fluid.

[0147] The pH of the fluid entering the second element may be estimated, using well-established relationships, by solving:

(1+Q13)(CH−Kw/CH)+CTFA+CHClQ13−CIMDQ13/(1+KIMD/CH)=0

[0148] for the H-ion concentration, CH, hence the pH. Here CTFA, CIMD and CHCl are the concentrations of TFA, imidazole and HCl in the first and second fluids, Kw and KIMD are the equilibrium constants for water and imidazole, respectively.

[0149] For illustration, but not limiting the range of operation of invention, for the case of P1=P1A, hence x=1, the ratio of flowrates is Q13=k1/k3 for all values of α. A design using a value of k1 that is 25% of k3 provides a mixture that buffers the working fluid to about pH 7.2 at the inlet 101 of the second element 100, a condition that yields high performance electrokinetics from silica. A further advantage is gained in that the conductivity of the liquid mixture is substantially reduced, since the high mobility H-ions in the acidic liquid have been replaced by significantly lower mobility imidazole ions in the liquid flowing through the second element 100.

[0150] It will be appreciated, by inspecting the relationship for pH, that values of the product CIMD Q13 must be about two times greater than CTFA to obtain pH values greater than about 7. It is thus preferable to employ a concentrated weak-base in the second fluid (a concentration substantially higher than the acid concentration in the working liquid) to allow for the use of small values of Q13. Obviously a strong base or a weak base with a very low equilibrium constant could be employed. However these in concentrated form yield a high pH second liquid that may damage materials. For example with 1 mM HCl and 100 mM aqueous tris(hydroxymethyl)aminomethane “TRIS” or imidazole, the pH values are about 10.4 or 9.15, respectively. The pH with the concentrated TRIS is sufficiently high to promote dissolution of silica. Whereas silica is reasonably stable at pH values less than about 9.5 making imidazole a viable candidate. Other weak bases may be equally employed.

[0151] Some notable advantages of flow controller systems, like the preceding embodiment of the invention, in which multiple fluids may be used are:

[0152] The ability to run with a wider range of fluid compositions and fluid conditions using a single electroosmotically active element. The composition of the second fluid may be altered to address different working fluids but no change to the physical device/system is required.

[0153] The ability to employ working fluids that are not suitable for electroosmotic flow. The mixture of the working fluid and the second fluid supports electrokinetic flow. Hence, the number of potential ‘working’ liquids includes those already discussed, but is also increased significantly. The working liquid preferably is miscible in and not reactive with the second liquid. For example, benzene, substituted benzenes, long chain aliphatics heptane, hexane, pentane, and carbon tetrachloride have relatively low permittivity and/or dipole moment and thus do not support electrokinetic flow. However these are miscible in isopropyl alcohol, for example, and the mixture can support electrokinetic flow.

[0154] The ability to use silica as the electrokinetically active element with a much greater number of working fluids, and thus take advantage of a well-characterized, widely available, and easily formed material having high electrokinetic performance.

[0155] The ability to use other high performance active materials (e.g. certain polymers or other metal oxides) under liquid conditions that provide for high performance and chemical stability of the materials.

[0156] The ability to use silica as a negative zeta potential material at low working fluid pH values, which are often employed in running buffers in HPLC of proteins and small molecules.

[0157] Electrokinetic operation over a well-defined range of pH, thus providing tolerance for and predictable operation with liquids bearing polyvalent ions.

[0158] The ability to use a high ionic strength, hence high electrical conductivity, working fluid. The zeta potential, hence performance, decreases and the electrical power dissipation hence Joule heating increases with increasing ionic strength of the working fluid. In such cases the second fluid is preferably selected to be of low ionic strength (nominally 0.1 to 1 millimolar) and preferably containing a relatively low specific conductivity salt or buffer. Mixing of the two fluids reduces the conductivity of the fluid in the active element, increases the zeta potential, and decreases Joule heating. There is no absolute limit to the strength of the ionic fluid that may be used. A system can be designed so that a fluid of any ionic strength fluid can be sufficiently diluted to support electrokinetic flow. However, the maximum flow rate for the working fluid will decrease in proportion to its ionic strength.

[0159] The ability to use pure solvent working fluids. Some ionic content is required to achieve reasonable electroosmotic performance. Pure solvents are thus poor electroosmotic fluids, particularly in small-pore size media due to problems with charge layer overlap. In such cases the second fluid is preferably selected to be of moderate ionic strength (10 to 100 mM, for example). Mixing of the two fluids provides the ionic content needed for high performance electrokinetics.

[0160] The ability to use pure organic working fluids. In many cases pure organic solvents, even with suitable ionic content, provide noticeably lower electrokinetic performance than the same solvents containing even a few percent water. In such cases, the second fluid preferably is aqueous and has a moderate ionic strength. Mixing of the two fluids provides the water content needed for high performance electrokinetics.

[0161] Another alternative embodiment configured in a shunt-mode is shown in FIG. 13. The first, second and third elements 205, 100, and 201 respectively of FIG. 13 play the same roles as the first, second and third elements 205, 100, and 201, respectively, of FIG. 12. The working fluid 1203 is supplied at pressure P1 to the inlet 609 of the fourth flow element 608. The outlet 611 of the fourth flow element 608 is connected at a second junction 610 with the inlet 203 of the third flow element 201 and the inlet 626 of a fifth flow element 625. A second fluid 1204 is supplied at pressure P1A to the inlet 206 of the first flow element 205. The outlet 207 of the first flow element 205 is connected at a first junction 202 with second and third flow elements 100 and 201, respectively. The second flow element 100 is electrokinetically active and terminates in a reservoir 1205 at pressure P2 that is less than P1A and P1. The second fluid 1204 mixes with the working fluid 1203 at junction 202 to yield a mixture providing acceptable electroosmotic performance of the controller. The fifth flow element 625 terminates at a terminus 1301, for example a chromatograph, at a pressure P3 that is less than P1A and P1. The objective is to control the flow of working fluid 1203 through the fifth element 625.

[0162] The pressures at the first and second junctions 202 and 610, respectively, P′ and P″ respectively, are determined by solution of:

(P1A−P′)k1+(P″−P′)k3=P′k2

(P1−P″)k4=(P″−P′)k3+(P″−P3)k5

[0163] where these relations, without any loss of generality, are written with respect to a P2 gauge pressure. Several conditions govern or suggest relationships between the various conductances of the flow elements.

[0164] For many applications, particularly in chemical analysis, a goal is to avoid contamination of the fluid flowing through the fifth element 625. In such cases, in a preferred design, the flow conductances are selected to direct the flow through the third element 201 from the second junction 610 to the first junction 202. This requires, for all positive values of α,

k 1 k 4 +k 2 k 4 +(k1k5+k2k5)P3/P1>(k1k4+k1k5)x

[0165] It is preferable to exceed this inequality by a factor of at least 1.2 and more preferably by 2 to 3 times. Higher values tend to minimize system-to-system, performance variation due to component element part-to-part variations.

[0166] Additionally, the third element 201 may be used to prevent the second fluid 1204 from contaminating the fluid flowing through the fifth element 625. This is preferably done with as little head loss as possible. Thus the conductance of the third element 201 is preferably much greater than that of the other elements. Preferably k3 is at least 100 times and more preferably about 1000 to 5000 times larger than the conductance of the other elements.

[0167] Preferably, the ratio of the flowrates through the first and third elements 205 and 201, respectively are set, thereby setting the ratio of the two fluids in the mixture reaching the second element 100. This ratio then allows fluid properties that affect the electrokinetic performance, such as pH or the amount of dilution, to be computed. For the case where P1A=P1 (this equality is imposed here for illustration and does not limit the general operation or applicability of the invention) 3$\frac{Q_1}{Q_3}=\frac{k_1}{k_3}\frac{k_2\left(k_3+k_4+k_5\right)\left(1+a\right)+k_3{k}_5\left(1-P_3/P_1\right)}{k_2{k}_4-k_5{k}_1+{\mathrm{ak}}_2\left(k_4+k_5\right)+k_5\left(k_1+k_2\right)P_3/P_1}$

[0168] Optionally, for example, a flow controller may be designed so that there is a maximum pressure available at the inlet 626 to the fifth element 625, a maximum or minimum flowrate through the fifth element and/or a maximum conductance of the fifth element. Given the conductances determined as described above, setting a minimum flowrate through the fifth element provides a maximum value for α.

[0169] In a specific example, P1A=P1, the working fluid 1203 is 10 mM aqueous TFA, the second fluid 1204 is aqueous 500 mM imidazole and 3 mM TFA, and the active element, which is the second element 100, is packed with nominal 0.6 micron non-porous silica particles. For positive flow through the third element 201, k2k4>k1k5. Inspection of the equations reveals that the lowest fraction of second fluid 1204 added to the mixture, a condition that will yield the most acidic pH, occurs for an applied potential to the second element 100 that yields a gauge pressure of zero at the first junction 202. In this limit the ratio of flowrates is: 4$\frac{Q_1}{Q_3}=\frac{k_1}{k_3}\frac{k_3+k_4+k_5}{k_4+k_5{P}_3/P_1}$

[0170] The design choices in this example with P3=0 suggest conductance values, relative to the value of k5, of about 0.19, 0.14, 3000 and 2.7 for k1 through k4 respectively. The entire set can be scaled to meet the flowrate requirements through the fifth element 625. With these values, the condition for positive flow through the third element 201 is well satisfied. The ratio of flowrates through the first and third element 205 and 201, respectively is sufficient to yield pH values at the inlet 101 of the second element 100 that are greater than about 7.2 over the entire operating range, thus providing for high performance electrokinetics with silica. This set of values also provides a maximum of about 70% of the working fluid source pressure at the inlet 626 of the fifth element 625.

[0171] In the embodiment illustrated in FIG. 13, the second fluid 1204 and the working fluid 1203 are combined at the first junction 202 and flow directly into the second element 100. This may not provide sufficient residence time to assure reasonably complete mixing of the two fluids 1203 and 1204. Some amount of mixing is preferred to obtain higher electroosmotic performance.

[0172] In the embodiment illustrated in FIG. 14, the second fluid 1204 and the working fluid 1203 are combined at the third junction 1401. The combined fluids then flow through a sixth element 1403, having an inlet 1405 and an outlet 1407, to the inlet 101 of the second element 100. The finite residence time of the combined fluids in the sixth element 1403 promotes mixing of the two fluids 1203 and 1204.

[0173] The flow is in the ‘creeping’ or ‘Stokes’ limit. As such, lateral mixing of the two fluids is by diffusion. Thus, the length of the sixth element 1403 preferably is substantially larger, more preferably at least 10 times, and most preferably between 100 and 500 times larger than the quantity Q6/2πD, where D is the diffusion coefficient of one fluid into the other. For cases where the sixth element 1403 is not of circular cross sectional shape the ‘diameter’ is preferably, taken as the major diameter of the non-circular shape.

[0174] Alternate methods may be employed to promote this mixing. For example, structures that serve to enhance stirring, such as passive or active mixers 1409, as are well known in the art, can be included within the sixth element 1403.

[0175] A pair of pressure sensors 301 and 311 are arranged to determine the flowrate through the fifth element 625. These signals can then be employed as part of a servo-loop to control the flowrate by actively adjusting the potential across the active element.

[0176] A number of occurrences may introduce an apparent compressibility (fractional change in volume with respect to a change in pressure) into the system and thereby affect the flowrate and flow direction. Such occurrences include, but are not limited to: the presence of a bubble of gas; isothermal compression of a fluid; and deflection of the sensor diaphragm.

[0177] During a substantial pressure transient, as might occur at first pressurization of the system, the presence of an apparent compressibility, for example, a sensor connected directly at the input of the fifth element 625, may temporarily alter the flow direction through the third element 201 of the embodiment illustrated in FIG. 13. Such a transient flow reversal may temporarily contaminate the second junction 610 with the second fluid 1204.

[0178] At initial pressurization, the second fluid 1204 flows through the first element 205 and then some portion may flow through the third element 201 to fill the compressible volume at junction 610. These flows persist until the sensor volume is pressurized, after which the overall flow through the third element 201 is directed from the second junction 610 to the first junction 202 (positive flow).

[0179] When k3 is selected to be substantially greater than the conductances of the other flow elements, α3/k1 is preferably greater than α2/k4, more preferably at least two times greater than α2/k4, and most preferably more than 5 to 10 times greater than α2/k4, in order to have proper flow direction in the third element 201 during the start-up transient. Here, α=θv, where v is the internal volume of a junction plus any attendant volume or sensor, θ is the sum of apparent compressibilities within the volume, and α2 and α3 are the α-values associated with the second and third junctions. It will be appreciated that the ratio α/k has dimensions of time and reflects a time-response in the same manner as an RC-time-constant in an electronic circuit.

[0180] It is generally preferable to minimize the α-values throughout the system to obtain faster system time response. Thus it is not preferable to satisfy the above condition by making α3 large, rather it is preferable to make α2 small in combination with selecting k4>k1, the latter being wholly consistent with the requirement for positive steady state flow through the third and sixth elements 201 and 1403, respectively.

[0181] Alternate methods for guaranteeing positive flow through the third and sixth elements 201 and 1403, respectively during a pressurization transient include but are not limited to:

[0182] during system pressurization, initiating pressure P1 before pressure P1A and during system de-pressurization, removing pressure P1A prior to removing pressure P1;

[0183] for cases were P1 and P1A are derived from the same source of pressure, a compressible volume, acting as an accumulator that will delay pressurization of the first element, may be added at the 206 inlet to the first element 205;

[0184] with the pressure transducer, which acts as a first accumulator 1411, at the second junction, installing a compressible volume, which acts as a second accumulator 1413, at the third junction to provide an appropriate 60 -value;

[0185] with the pressure transducer at the second junction, installing a check valve to direct flow from the second to the third junction.

[0186] The devices shown thus far employ a single active element 100 and rely on prudent selection of conductances to passively control the ratio of the fluids in the mixture reaching the active element. Active control of other component conductances and driving pressures can add flexibility and can serve to loosen design constraints. FIG. 15 shows two electrokinetically active elements in series as part of a shunt-mode flow controller.

[0187] For the shunt-mode controller with mixing, inspection of the equations reveals that the lowest value of the ratio Q1/Q3 occurs for the lowest value of the pressure at the first junction 202. Consider the shunt-mode controller of FIG. 15 included in a mixing system such as the one shown in FIG. 13. For illustration, but not limiting the invention, the lowest operating pressure at the first junction 202 is taken as zero, the pressure P3 is taken as zero, and P1A is taken equal to P1. The ratio of flowrates, in the limit that k3 is substantially greater than the other k-values, is then Q1/Q3=(1+a1max) k1/k4, where a1max is the value of a1 for the first element 205 at the voltage needed to reach zero pressure in the first junction 202. The condition for positive flow through the third element 201 (for the case considered in this illustration) is k2k4>k1k5 and generally dynamic range considerations tend to yield values of k2 less than or about equal to k5. Thus, the need to provide a finite ratio of flowrates requires, in this example, a finite value of k1/k4 whereas the condition for positive flow through the third element 201 requires, in this example, reducing the value of k1/k4. A finite positive value of a1max can be used to enhance the ratio of flowrates and thus make it possible to satisfy these opposing conditions on the relative values of k1 and k4. It is preferred that the value of a1 be less than the value of a2, preferably 5 to 10 time less, otherwise the electroosmotic flow through the first element 205 will overwhelm that through the second element 100 and the system will not be able to control the flow through the fifth element 625.

[0188] In FIG. 15A, the two active elements 100 and 205 have separate power supplies 107 and 1501, respectively, Accordingly the respective electroosmotic flowrates are controlled independently. This control may employ various algorithms possibly enhanced by measuring supply currents or using various sensor inputs.

[0189] In FIG. 15B, a single supply is employed such that a common current is carried through the two active elements 100 and 205, respectively, which are connected electrically in series. In this configuration akP1=sI, where s=ν/σ, σ is the electrical conductivity of the fluid as modified by any porous media present, and I is the electrical current. With the common current k1a1/k2a2=S1/S2. The requirement for a1 less than a2 can be satisfied by selection of the material in the first element 205 to yield an appropriate value of the zeta potential with respect to that of the second element 100.

[0190] Flow controllers having multiple active elements have been described in light of the fluid mixing configurations revealed. However, such active control schemes are also applicable to and can provide increased range of operation in other flow controller system embodiments that have multiple fluid sources and flow controller system embodiments that have a single fluid.

[0191] FIG. 16 shows a passive design where sixth and seventh flow elements 1602 and 1607, respectively are connected in series between the source 1201 of the second fluid 1204 supplied at pressure P1A and a drain 1601 at pressure P2A that is less than pressures P1 and P1A. The inlet 206 to the first element 205 is connected to the common junction 1610 of sixth and seventh elements 1602 and 1607, respectively. This combination allows the pressure at the inlet 206 of the first element 205 to be reduced from the second fluid source pressure in a fashion much like the use of a resistive voltage divider. This configuration is useful when one wants to use a single pressure source to drive two systems with different pressures.

[0192] Alternatively, the sixth element 1602 may be electrokinetically active. This configuration allows the pressure at the junction 1610 to be modulated by varying the potential applied to the sixth element 1602. The two active elements in this embodiment preferably have separate power supplies allowing the respective electroosmotic flowrates to be controlled independently. This embodiment may employ various algorithms possibly enhanced by measuring supply currents or using various sensor inputs.

[0193] In the embodiments of flow controllers that are used in conjunction with multiple fluids described thus far, the working fluid is mixed with a second fluid that supports electrokinetic activity. Sometimes, it is desirable that the working fluid not mix with another fluid.

[0194] The embodiment shown in FIG. 17 may be used when this is the case. A charge of working fluid 1203 is stored within the volume of the fifth element 625 also sometimes referred to as a fluid storage element or cartridge. The working fluid 1203 is supplied through the fourth element 608 and then to the terminus 1301 by displacing the working fluid 1203 within the fifth element 625 with the second liquid 1204 supplied through the third element 201. The flowrate of working fluid 1203 is thus controlled by controlling the flowrate of the second liquid 1204 through the third element 201.

[0195] The first, second and third elements 205, 100 and 201, respectively form a shunt-mode electroosmotic flow controller. This second liquid 1204 is not necessarily the same as the working fluid 1203 and is selected to support the production of a zeta potential in the second element 100. In this configuration, the number of potential working liquids increases dramatically as the working liquid does not need to be miscible with the second liquid. Nor does the mixture need not support electrokinetic flow. Preferably, however the working liquid is not reactive with the second liquid.

[0196] Working liquids include, but are not limited to all of the working liquids previously listed, oils, hydraulic fluids, gases, slurries (i.e. liquids bearing particulates), emulsions, refrigerants, CFC's, supercritical liquids or mixtures thereof.

[0197] The system of FIG. 17 has a time-of-operation limited by the amount of working fluid 1203 stored within the fifth element 625. This time-of-operation may be reduced by any mixing of the two fluids 1203 and 1204 within the fifth element 625. To this end it is preferable that the flow in the fifth element 625 be laminar and that the geometry of the fifth element 625 be selected for a hydraulic diameter that is substantially less than the length of the element, e.g. a length of fine-bore tubing. It is further preferable that the hydraulic diameter be selected to yield a small value of the Peclet number, of the order 0.5 to 20, which is about equal to the product of hydraulic diameter and flow mean velocity divided by twice the diffusion coefficient of one fluid into the other. The use of a fine-bored tube as a liquid storage volume and the use of such a tube inserted in a running stream as a means of dispensing the liquid is well-known and widely used in the arts of liquid chromatography. See, e.g., A. Weston and P. R. Brown, HPLC and CE: Principles and Practice, Academic Press, San Diego, Calif., 1997, pp. 83-84. The particulars of evaluating the degree of and controlling mixing in this type of flow and geometry are well known in the arts of mechanical engineering. See, e.g., V. Ananthakrishnan, W. N. Gill and A. J. Barduhn, ‘Laminar Dispersion in Capillaries, Part 1. Mathematical Analysis,’ AIChE J. Vol. 11 pp. 1063-1072 (1965). G. I. Taylor, ‘Dispersion of a solute flowing through a tube,’ Proc. Roy. Soc. (London) Vol. 219A, pp. 186-203 (1953). R. Aris, ‘On the dispersion of soluble matter in solvent flowing slowly through a tube,’ Proc. Roy. Soc. (London) Vol. 235A, pp. 67-77 (1956). P. C. Chatwin and P. J. Sullivan ‘The effect of aspect ratio on the longitudinal diffusivity in rectangual channels,’ J. Fluid Mech. Vol. 120, pp. 347-358 (1982). M. R. Doshi, P. M. Daiya and W. N. Gill, ‘Three dimensional laminar dispersion in open and closed rectangular ducts,’ Chem. Eng. Sci. Vol 33, pp. 795-804 (1978).

[0198] The fifth element 625 of FIG. 17 may be equipped with valving at either end as a means of switching the fifth element in and out of the flow circuit so as to allow flushing of the fifth element and replenishment of the working fluid 1203. FIG. 18 shows a valve 1801 configuration. The valve 1801 may be a rotary valve or a collection of discrete valves or any other configuration known in the arts. The valve 1801 may incorporate one or two or more storage volumes and may be ganged to provide like service to more than one flow controller.

[0199] In the embodiment shown in FIG. 18 two working fluid storage volumes 625 and 1602 are in operative association with the valve 1801. These two elements 625 and 1602 need not be identical or have the same internal volume. The working fluids 1203 in these two elements 625 and 1602 need not be the same.

[0200] The flow through the third element 201 is routed through the fifth element 625 and then through the fourth element 608. The sixth element 1602 is shown connected between the source 1803 and the drain 1804 of the working fluid 1203. In this configuration, the working fluid 1203 from the fifth element 625 is supplied to the inlet 609 of the fourth element 608 while the sixth element 1602 is flushed and filled with new working fluid 1203. At some selected time the valve 1801 is actuated, 1/8 turn counterclockwise for the device of FIG. 18, to switch the rolls of the fifth and sixth elements 625 and 1602, respectively. At some selected later time the process is reversed and so on. This allows continuous delivery of the working fluid 1203 or switching of the working fluids, with some minor disruption during valve actuation.

[0201] The flowrate and the volume of the stored liquid determine the maximum time of operation, whereas the size of the volume and the conductances of the connected elements determine the response time of the device. It is thus preferable to minimize the size of the storage volume and provide means to switch-in a newly filled storage volume.

[0202] For example, in the system as shown in FIG. 17, equipped with a rotary valve element as shown in FIG. 18, used for delivery of a working fluid for chemical synthesis, the working fluid can be acetonitrile possibly containing some small amount of an organic acid, e.g. formic or acetic acid. A controlled flowrate of working fluid in the range of 100 to 500 nL/min at load pressures in the range of zero to 25 psi above ambient, for example, is desired. The second liquid 1209 can be aqueous 10 millimolar TRIS and about 5 millimolar acetic acid. The source pressure P1 can be between about 500 and 600 psi. The second element 100 can be a 3 cm long, 150 micron inner diameter, “ID,” capillary filled with nominal 0.7 micron diameter non-porous silica beads. The first, third and fourth elements 205, 201 and 608, respectively can be simple capillaries. The fifth element 625, and sixth element 1602, if used, can be a length of 0.03 inch ID tubing. The pressure difference across the fourth element 608 can be used to monitor the flowrate.

[0203] A design using conductances for the first and fourth elements 205 and 608, of about 1.8 and 5 nl/min-psi, hence lengths of 10 micron ID capillary of about 6.2 and 5.7 cm, provides for a desired range of delivery pressure and flowrate using potentials applied to the second element 100 in the range of about 0.95 and 2.5 kV. The third element 201 can be simply a length of tube or capillary having a substantially larger conductance than the first, second and fourth elements 205, 100, and 608, respectively. The third element 201 can serve several roles: a connector between the first junction 202 and the valve 1801; provide electrical isolation between the bridge connection 108 and the valve 1801; and minimize any back diffusion or mixing of working fluid 1203 into the first junction 202 that might occur during start-up or switching of the valve 1801. The length of the fifth element 625 can be selected to be about 110 cm thereby providing about 16 hours of uninterrupted run-time at the maximum delivery flowrate in this example. The time constant for this embodiment is about 15 seconds. In this design, the roles of the third and fourth elements 201 and 608, respectively, may be reversed.

[0204] In an alternative embodiment, the storage element may be placed before the active flow controller element. Such a configuration is shown in FIG. 19. The working fluid 1203 is supplied at pressure P1 and passes through the first and fourth elements 205 and 608, respectively. The fluid storage element 625 is placed before the electroosmotically active second element 100 and filled with a second fluid 1204 that is designed to support the electroosmotic function of the second element. As the device is operated, the working fluid 1203 displaces the second fluid 1204 stored in the fifth element 625. This embodiment is subject to the same time response and time-of-operation limitations as the previously discussed embodiment. It may also be used with switchable valves and multiple storage elements. One benefit of this embodiment is that the second fluid 1204 is never present in the working fluid 1203 delivery stream and therefore cannot contaminate the output fluid. Instead, the potential for accidental contamination is transferred to the second element 100, which may be more acceptable in certain applications.

[0205] Placing a storage element before the electroosmotically active element is also easily realized in a series-mode controller format, as depicted in FIG. 20. The fifth element 625 serves as the storage element for fluid 1204 that supports the electroosmotically active second element 100.

[0206] Although the present invention has been described in considerable detail with reference to certain preferred versions thereof, other versions are possible. For example, a flow controller system having features of the present invention can include three fluid sources. Therefore, the spirit and scope of the appended claims should not be limited to the description of the preferred versions contained herein.

Claims

1. A flow controller system, comprising: (a) a channel having: (i) a fluid inlet in fluid communication with a first fluid source at pressure P1, and a second fluid source at pressure P2; (ii) a fluid outlet in fluid communication with the fluid inlet and at pressure P3, with a first fluid terminus, wherein P3<P1 and P3<P2; and (iii) a porous dielectric material disposed in the channel; and (b) a power supply in electrical communication with spaced electrodes for applying an electric potential to the electrodes, the electrodes being positioned so that the channel is electrokinetically active when the power supply applies an electric potential to the electrodes; whereby the electric potential generates an electroosmotically-driven flow component through the channel that modulates at least one pressure-driven flow component resulting from the P1−P3 pressure differential and the P2−P3 pressure differential.

2. The system of claim 1, wherein the power supply is a variable power supply.

3. The system of claim 1, wherein the pressure-driven and the electroosmotically-driven flow components through the channel are in the same direction.

4. The system of claim 1, wherein the pressure-driven and the electroosmotically-driven flow components through the channel are in the opposite direction and the pressure-driven fluid flux is greater than or equal to the electroosmotically driven fluid flux.

5. The system of claim 1, wherein the electrical communication is through a bridge.

6. The system of claim 1, wherein the first fluid terminus is a chromatograph.

7. The system of claim 1, wherein the channel comprises a fused silica capillary.

8. The flow controller of claim 1, wherein the porous dielectric material includes porous dielectric materials fabricated by processes selected from the group consisting of lithographic patterning and etching, direct injection molding, sol-gel processing, and electroforming.

9. The flow controller of claim 1, wherein the porous dielectric material includes organic polymer materials.

10. The system of claim 1, wherein one of the fluid sources supplies a fluid having an ionic strength of at least 25 millimolar to the system.

11. The system of claim 1, wherein one of the fluid sources supplies a fluid having an ionic strength less than 0.5 millimolar to the system.

12. The system of claim 1, wherein one of the fluid sources supplies a fluid having a dynamic viscosity greater than 5 centipoise.

13. The system of claim 1, wherein one of the fluid sources supplies a substantially pure organic fluid to the system.

14. The system of claim 1, wherein one of the fluid sources supplies a fluid having dielectric constant less than 20 to the system.

15. The system of claim 1, wherein one of the fluid sources supplies a fluid bearing polyvalent ions to the system.

16. The system of claim 1, wherein the porous dielectric material includes silica particles.

17. The system of claim 16, wherein one of the fluid sources supplies a fluid having a pH value <7 to the system.

18. The system of claim 16, wherein one of the fluid sources supplies a fluid having a pH value <4 to the system.

19. The system of claim 1, further comprising at least one sensor for monitoring at least one control signal, and a feedback control mechanism operatively connected to the sensor and the power supply, wherein the feedback control mechanism maintains the at least one control signal within a predetermined range by modulating the electric potential applied by the power supply.

20. The system of claim 19, wherein the at least one sensor is selected from the group consisting of a pressure transducer, a flowmeter, a temperature sensor, a heat flux sensor, a displacement sensor, a load cell, a strain gauge, a conductivity sensor, a selective ion sensor, a pH sensor, a flow spectrophotometer, and a turbidity sensor.

21. A flow controller system, comprising: (a) a channel having: (i) a fluid inlet in fluid communication with a first fluid source at pressure P1, and a second fluid source at pressure P2; (ii) a fluid outlet in fluid communication with the fluid inlet and a first fluid terminus at pressure P3, wherein P3<P1 and P3<P2; and (iii) a porous dielectric material disposed in the channel; and (b) a power supply in electrical communication with spaced electrodes for applying an electric potential to the electrodes, the electrodes being positioned so that the channel is electrokinetically active when the power supply applies an electric potential to the electrodes and; (c) a first flow element interposed between the first fluid source and a first node, the first flow element having a first flow element inlet in fluid communication with the first fluid source, the first flow element also having a first flow element outlet in fluid communication with the first flow element inlet and, at the first node at pressure PN1, with the fluid inlet, wherein P3<PN1; whereby the electric potential generates an electroosmotically-driven flow component through the channel that modulates at least one pressure-driven flow component resulting from the P1−P3 pressure differential and the P2−P3 pressure differential.

22. The system of claim 21, further comprising: (d) a second flow element interposed between the second fluid source and the first node, the second flow element having a second flow element inlet in fluid communication with the second fluid source, the second flow element also having a second flow element outlet in fluid communication with the second flow element inlet and, at the first node, with the fluid inlet; wherein the channel is also a third flow element.

23. The system of claim 22 wherein the first flow element has a conductance k1, the second flow element has a conductance k2, the third flow element has a conductance k3 and 1+k3/k1>P1/P2 and 1+k3/k2>P2/P1.

24. The system of claim 22, further comprising: (e) a fourth flow element interposed between the first node and the third element, the fourth flow element having a fourth flow element inlet in fluid communication at the first node with the first flow element outlet and the second flow element outlet, the fourth flow element having a fourth flow element outlet in fluid communication with the fourth flow element inlet and the third flow element inlet.

25. A flow controller system, comprising: (a) a channel having: (i) a fluid inlet in fluid communication with a first fluid source at pressure P1, and a second fluid source at pressure P2; (ii) a fluid outlet in fluid communication with the fluid inlet and, at pressure P3, with a first fluid terminus, wherein P3<P1 and P3<P2; and (iii) a porous dielectric material disposed in the channel; (b) a power supply in electrical communication with spaced electrodes for applying an electric potential to the electrodes, the electrodes being positioned so that the channel is electrokinetically active when the power supply applies an electric potential to the electrodes; (c) a first flow element interposed between the first fluid source and a first node, the first flow element having a first flow element inlet in fluid communication with the first fluid source, the first flow element also having a first flow element outlet in fluid communication with the first flow element inlet and, at the first node at pressure PN1, with the fluid inlet, wherein P3<PN1; (d) a second flow element interposed between the second fluid source and the first node, the second flow element having a second flow element inlet in fluid communication with the second fluid source, the second flow element also having a second flow element outlet in fluid communication with the second flow element inlet and, at the first node, with the fluid inlet; wherein the channel is also a third flow element; (e) a fourth flow element interposed between the first node and the third element, the fourth flow element having a fourth flow element inlet in fluid communication at the first node with the first flow element outlet and the second flow element outlet, the fourth flow element also having a fourth flow element outlet in fluid communication with the fourth flow element inlet and the third flow element inlet; and (f) a fluid mixer located in the fourth flow element; whereby the electric potential generates an electroosmotically-driven flow component through the channel that modulates at least one pressure-driven flow component resulting from the P1−P3 pressure differential and the P2−P3 pressure differential.

26. The system of claim 22 further comprising: (e) a second fluid terminus at pressure P4, wherein P4<P1, the second fluid terminus being in fluid communication at a second node at pressure PN2, wherein P3<PN2, and P4<PN2 with the first fluid source and the first flow element inlet; (f) a fourth flow element interposed between the first fluid source and the second node, the fourth flow element having a fourth flow element inlet in fluid communication with the first fluid source, the fourth flow element also having a fourth flow element outlet in fluid communication with the fourth flow element inlet and, at the second node at pressure PN2, with the first flow element inlet and the second fluid terminus; and (g) a fifth flow element interposed between the second node and the second fluid terminus, the fifth flow element having a fifth flow element inlet in fluid communication at the second node with the fourth flow element outlet, the fifth flow element also having a fifth flow element outlet in fluid communication with the fifth flow element inlet and the second fluid terminus.

27. The system of claim 26 wherein one of the fluid terminuses is a chromatograph.

28. The system of claim 26 further comprising an accumulator located in the second flow element inlet.

29. The system of claim 26, further comprising an accumulator at the first node.

30. The system of claim 26, further comprising an accumulator between the first fluid source and the inlet to the first flow element.

31. A flow controller system, comprising: (a) a channel having: (i) a fluid inlet in fluid communication with a first fluid source at pressure P1, and a second fluid source at pressure P2; (ii) a fluid outlet in fluid communication with the fluid inlet and, at pressure P3, with a first fluid terminus wherein P3<P1 and P3<P2; and (iii) a porous dielectric material disposed in the channel; (b) a power supply in electrical communication with spaced electrodes for applying an electric potential to the electrodes, the electrodes being positioned so that the channel is electrokinetically active when the power supply applies an electric potential to the electrodes; (c) a first flow element interposed between the first fluid source and a first node, the first flow element having a first flow element inlet in fluid communication with the first fluid source, the first flow element also having a first flow element outlet in fluid communication with the first flow element inlet and, at the first node at pressure PN1, with the fluid inlet, wherein P3<PN1; (d) a second flow element interposed between the second fluid source and the first node, the second flow element having a second flow element inlet in fluid communication with the second fluid source, the second flow element also having a second flow element outlet in fluid communication with the second flow element inlet and, at the first node, with the fluid inlet; (e) a second fluid terminus at pressure P4, wherein P4<P1, the second fluid terminus being in fluid communication at a second node at pressure PN2, wherein P3<PN2, and P4<PN2 with the first fluid source and the first flow element inlet; wherein the channel is also a third flow element; (f) a fourth flow element interposed between the first fluid source and the second node, the fourth flow element having a fourth flow element inlet in fluid communication with the first fluid source, the fourth flow element also having a fourth flow element outlet in fluid communication with the fourth flow element inlet and, at the second node at pressure PN2, with the first flow element inlet and the second fluid terminus; (g) a fifth flow element interposed between the second node and the second fluid terminus, the fifth flow element having a fifth flow element inlet in fluid communication at the second node with the fourth flow element outlet, the fifth flow element also having a fifth flow element outlet in fluid communication with the fifth flow element inlet and the second fluid terminus; (h) a third fluid terminus at pressure P5, wherein and P5<P2, the third fluid terminus being in fluid communication at a third node with the second fluid source and the second flow element inlet; (i) a sixth flow element interposed between the third fluid terminus and the third node, the sixth flow element having a sixth flow element inlet in fluid communication at the third node with the second fluid source, the sixth flow element also having a sixth flow element outlet in fluid communication with the sixth flow element inlet and the third fluid terminus; and (j) a seventh flow element interposed between the second fluid source and the third node, the seventh flow element having a seventh flow element inlet in fluid communication with the second fluid source, the seventh flow element also having a seventh flow element outlet in fluid communication with the seventh flow element inlet and, at the third node, with the sixth flow element inlet and the second flow element inlet; whereby the electric potential generates an electroosmotically-driven flow component through the channel that modulates at least one pressure-driven flow component resulting from the P1−P3 pressure differential and the P2−P3 pressure differential.

32. The system of claim 31, further comprising: (k) a porous dielectric material disposed in the sixth flow element; (l) a second power supply in electrical communication with the second set of spaced electrodes for applying an electric potential to the second set of spaced electrodes, the second set of spaced electrodes being positioned so that the sixth flow element is electrokinetically active when the second power supply applies an electric potential to the second set of spaced electrodes.

33. The system of claim 26, wherein the second flow element has a conductance k2, the third flow element has a conductance k3, the fourth flow element has a conductance k4, the fifth flow element has a conductance k5, and k2k4+k3k4+(k2k5+k3k5)P4/P1>(k2k4+k2k5)P2/P1.

34. The system of claim 26, wherein the first flow element has a conductance k1, and k1>k2, k3, k4, and k5.

35. The system of claim 34, wherein k1 is more than 100 times greater than each of k2, k3, k4, and k5.

36. The system of claim 26, further comprising: (h) a sixth flow element interposed between a third node at pressure PN3, wherein P3<PN3 and the first node, the sixth flow element having a sixth flow element inlet in fluid communication at the first node with the second flow element outlet and the first flow element outlet, the sixth flow element also having a sixth flow element outlet in fluid communication with the sixth flow element inlet and, at the third node, with the third element inlet.

37. The system of claim 36, wherein the first flow element has a conductance of k1 the second flow element has a conductance of k2, the third flow element has a conductance of k3, the fourth flow element has a conductance of k4, the fifth flow element has a conductance of k5 and the sixth flow element has a conductance of k6, and wherein k1+k6> each of k2, k3, k4 and k5.

38. The system of claim 26, wherein α1=θ1V1, where V1 is the internal volume of the first node and θ1 is the sum of apparent compressibilities within V1, α2=θ2V2 where V2 is the internal volume of the second node and θ2 is the sum of apparent compressibilities within V2, and wherein α1/k2>α2/k4.

39. The system of claim 36, wherein D represents the diffusion coefficient of the second fluid into the first fluid and the sixth element has a flowrate Q6 and a length L and L>Q6/2πD.

40. The system of claim 36, further comprising a fluid mixer located in the sixth flow element.

41. The system of claim 26, further comprising at least one sensor for monitoring at least one control signal, and a feedback control mechanism operatively connected to the sensor and the power supply, wherein the feedback control mechanism maintains the at least one control signal within a predetermined range by modulating the electric potential applied by the power supply.

42. The system of claim 41, wherein the at least one sensor is a pair of pressure transducers arranged to determine the flowrate through the fifth flow element.

43. The system of claim 41, wherein a pressure transducer is located at the first node.

44. The system of claim 41, wherein a pressure transducer is located at the second node, further comprising an accumulator located at the first node.

45. The system at claim 41, wherein a pressure transducer is located at the second node, further comprising a check valve located between the first and second nodes.

46. A flow controller system, comprising: (a) a first conduit having: (i) a first fluid inlet in fluid communication with a first fluid source at pressure P1; (ii) a first fluid outlet at pressure P3 in fluid communication with the first fluid inlet, wherein P3<P1; and (iii) a first flow element disposed between the first fluid inlet and a first node; and (b) a second conduit having: (i) a second fluid inlet in fluid communication with a second fluid source at pressure P2, wherein P3<P2; (ii) a second fluid outlet in fluid communication with the second fluid inlet and, at the first node, with the first conduit; (iii) a second flow element disposed between the second fluid inlet and the second fluid outlet; and (iv) a third fluid outlet at pressure P4, wherein P4<P1 and P4<P2, the third fluid outlet being in fluid communication at a second node with the second flow element outlet; wherein α1=θ1V1, where V1 is the internal volume of the first node and θ1 is the sum of apparent compressibilities within V1, α2=θ2V2 where V2 is the internal volume of the second node and θ2 is the sum of apparent compressibilities within V2, the first flow element has a conductance of k1, the second flow element has a conductance of k2, and wherein α1/k1>α2/k2.

47. A flow controller system, comprising: (a) a first conduit having: (i) a first fluid inlet in fluid communication with a first fluid source at pressure P1; (ii) a first fluid outlet at pressure P3 in fluid communication with the first fluid inlet, wherein P3<P1; and (iii) a first flow element disposed between the first fluid inlet and a first node; and (b) a second conduit having: (i) a second fluid inlet in fluid communication with a second fluid source at pressure P2, wherein P3<P2; (ii) a second fluid outlet in fluid communication with the second fluid inlet and, at the first node, with the first conduit; (iii) a second flow element disposed between the second fluid inlet and the second fluid outlet; and (iv) a third fluid outlet at pressure P4, wherein P4<P1 and P4<P2, the third fluid outlet being in fluid communication at a second node at pressure PN2, with the second flow element outlet; (c) a pressure transducer located at either the first or the second node; and (d) an accumulator located at the opposite node as the pressure transducer; wherein α1=θ1V1, where V1 is the internal volume of the first node and θ1 is the sum of apparent compressibilities within V1, α2=θ2V2 where V2 is the internal volume of the second node and θ2 is the sum of apparent compressibilities within V2, the first flow element has a conductance of k1, the second flow element has a conductance of k2, and wherein α1/k1>α2/k2.

48. A flow controller system, comprising: (a) a first conduit having: (i) a first fluid inlet in fluid communication with a first fluid source at pressure P1; (ii) a first fluid outlet at pressure P3 in fluid communication with the first fluid inlet, wherein P3<P1; and (iii) a first flow element disposed between the first fluid inlet and a first node; and (b) a second conduit having: (i) a second fluid inlet in fluid communication with a second fluid source at pressured P2, wherein P3<P2; (ii) a second fluid outlet in fluid communication with the second fluid inlet and, at the first node, with the first conduit; (iii) a second flow element disposed between the second fluid inlet and a second fluid outlet; and (iv) a third fluid outlet at pressure P4, wherein P4<P1 and P4<P2, the third fluid outlet being in fluid communication at a second node with the second flow element outlet; (c) a pressure transducer located at either the first or the second node; and (d) a check valve between the first and second nodes; wherein α1=θ1V1, where V1 is the internal volume of the first node and θ1 is the sum of apparent compressibilities within V1, α2−θ2V2 where V2 is the internal volume of the second node and θ2 is the sum of apparent compressibilities within V2, the first flow element has a conductance of k1, the second flow element has a conductance of k2, and wherein α1/k2>α2/k2.

49. A flow controller system, comprising: (a) a first channel having: (i) a first channel fluid inlet in fluid communication at a node with a first fluid source at pressure P1 and a second fluid source at pressure P2; (ii) a first channel fluid outlet in fluid communication with the first channel fluid inlet and, at pressure P3, with a fluid terminus, wherein P3<P1 and P3<P2; and (iii) a porous dielectric material disposed in the first channel; (b) a second channel having: (i) a second channel fluid inlet in fluid communication with the second fluid source; (ii) a second channel fluid outlet in fluid communication with the second channel fluid inlet and, at the first node, with the first channel inlet; and (iii) a porous dielectric material disposed in the second channel; and (c) a power supply in electrical communication with spaced electrodes for applying an electrical potential to the electrodes, the electrodes being positioned so that the channels are electrokinetically active when the power supply applies an electric potential to the electrodes; wherein the electric potential generates an electroosmotically-driven flow component through at least one of the first and the second channels, wherein the electroosmotically-driven flow component modulates at least one pressure-driven flow component resulting from the P1−P3 and the P2−P3 pressure differentials.

50. A flow controller system, comprising: (a) a first channel having: (i) a first channel fluid inlet in fluid communication at a first node with a first fluid source at pressure P1 and a second fluid source at pressure P2; (ii) a first channel fluid outlet in fluid communication with the first channel fluid inlet and, at pressure P3, with a fluid terminus, wherein P3<P1 and P3<P2; and (iii) a porous dielectric material disposed in the first channel; (b) a second channel having: (i) a second channel fluid inlet in fluid communication with the second fluid source; (ii) a second channel fluid outlet in fluid communication with the second channel fluid inlet and, at the first node, with the first channel; and (iii) a porous dielectric material disposed in the second channel; (c) a first power supply in electrical communication with a first set of spaced electrodes for applying a first electric potential to the first set of spaced electrodes, the first set of spaced electrodes being positioned so that the first channel is electrokinetically active when the first power supply applies an electric potential to the first set of spaced electrodes; (d) a second power supply in electrical communication with a second set of spaced electrodes for applying a second electric potential to the second set of spaced electrodes, the second set of spaced electrodes being positioned so that the second channel is electrokinetically active when the second power supply applies an electric potential to the second set of spaced electrodes; wherein the first electric potential generates a first electroosmotically-driven flow component through the first channel, the first electroosmotically-driven flow component modulating at least one pressure-driven flow component resulting from the P1−P3 and the P2−P3 pressure differentials and the second electric potential generates a second electroosmotically-driven flow component through the second channel, the second electroosmotically-driven flow component modulating at least one pressure-driven flow components resulting from the P1−P3 and the P2−P3 pressure differentials.

51. A flow controller system, comprising: (a) a channel having: (i) a fluid inlet in fluid communication at a node with a fluid source at pressure P1; (ii) a fluid outlet in fluid communication with the fluid inlet and, at pressure P2, with a first fluid terminus, wherein P2<P1; and (iii) a porous dielectric material disposed in the channel; (b) a power supply in electrical communication with spaced electrodes for applying an electric potential to the spaced electrodes, the spaced electrodes being positioned so that the channel is electrokinetically active when the power supply applies an electric potential to the electrodes; and (c) a first fluid storage element being disposed between the node and a second fluid terminus at pressure P3, wherein P3<P1, wherein the first fluid storage element has a first fluid storage element inlet in fluid communication at the node with the fluid source, and wherein the first fluid storage element also has a first fluid storage element outlet in fluid communication with the first fluid storage element inlet and the second fluid terminus; wherein the electric potential generates an electroosmotically-driven flow component through the channel that modulates at least one pressure-driven flow component resulting from the P1−P2 and the P1−P3 pressure differentials.

52. The system of claim 51, further comprising: (d) a first flow element disposed between the fluid source and the node, the first flow element having a first flow element inlet in fluid communication with the fluid source, the first flow element also having a first flow element outlet in fluid communication with the first flow element inlet and, at the first node, with the first fluid storage element inlet and the fluid inlet; wherein the channel is also a second flow element; (e) a third flow element disposed between the node and the first fluid storage element, the third flow element having a third flow element inlet in fluid communication at the first node with the first flow element outlet, the third flow element also having a third flow element outlet in fluid communication with the third flow element inlet and the first fluid storage element inlet; and (f) a fourth flow element disposed between the first fluid storage element and the second fluid terminus, the fourth flow element having a fourth flow element inlet in fluid communication with the first fluid storage element outlet, the fourth flow element also having a fourth flow element outlet in fluid communication with the fourth flow element inlet and the second fluid terminus; wherein the fluid storage element is also a flow element.

53. The system of claim 51, further comprising: (d) a second fluid storage element; and (e) a valve for switching the first fluid storage element with the second fluid storage element.

54. A flow controller system, comprising: (a) a channel having: (i) a fluid inlet in fluid communication at a node with a fluid source at pressure P1; (ii) a fluid outlet in fluid communication with the fluid inlet and, at pressure P2, with a first fluid terminus, wherein P2<P1; and (iii) a porous dielectric material disposed within the first channel; (b) a power supply in electrical communication with the spaced electrodes for applying an electrical potential to the spaced electrodes, the spaced electrodes being positioned so that the channel is electrokinetically active when the power supply applies an electric potential to the electrodes; (c) a first fluid storage element disposed between the node and the fluid inlet, the first fluid storage element having a first fluid storage element inlet in fluid communication at the node with the fluid source, the first fluid storage element also having a first fluid storage element outlet in fluid communication with the first fluid storage element inlet and the fluid inlet; and (d) a second fluid terminus at pressure P3, wherein P3<P1, in fluid communication at the node with the fluid source, wherein the electric potential generates an electroosmotically-driven flow component through the first channel that modulates at least one pressure-driven flow component resulting from the P1−P2 and the P1−P3 pressure differentials.

55. The system of claim 54, further comprising: (e) a first flow element disposed in the first channel between the first fluid source and the node, the first flow element having a first flow element inlet in fluid communication with the fluid source, the first flow element also having a first flow element outlet in fluid communication at the node with the first flow element inlet, the fluid storage element inlet, and the second fluid terminus; wherein the channel is also a second flow element; and (f) a third flow element disposed between the node and the second fluid terminus, the third flow element having a third flow element inlet in fluid communication at the node with the first flow element outlet, the third flow element also having a third flow element outlet in fluid communication with the third flow element inlet and the second fluid terminus.

56. The system of claim 54, further comprising: (e) a second fluid storage element; and (f) a valve for switching the first fluid storage element with the second fluid storage element.

57. A flow controller system, comprising: (a) a channel having: (i) a fluid inlet in liquid communication with a fluid source at pressure P1; (ii) a fluid outlet in liquid communication with a first fluid terminus at pressure P2, wherein P2<P1; and (iii) a porous dielectric material disposed in the channel; (b) a power supply in electrical communication with spaced electrodes for applying an electric potential to the spaced electrodes, the spaced electrodes being positioned so that the channel is electrokinetically active when the power supply applies an electric potential to the electrodes; and (c) a fluid storage element fluid disposed between the fluid source and the channel, the fluid storage element having a fluid storage element inlet in fluid communication with a fluid source, the fluid storage element also having a fluid storage element outlet in fluid communication with the fluid storage element inlet and the fluid inlet; whereby the electric potential generates an electroosmotically-driven flow component through the channel that modulates a pressure-drive flow component resulting from the P1−P2 pressure differential.

58. A method for controlling a flow of a fluid, comprising: applying an electric potential to spaced electrodes in electrical communication with a channel, the channel having a porous dielectric material disposed therein, the channel also having a fluid inlet in fluid communication with a first fluid source at pressure P1 and a second fluid source at pressure P2, the channel also having a fluid outlet in fluid communication with the fluid inlet and, at pressure P3, with a terminus, wherein P3<P1 and P3<P2, wherein the electric potential generates an electroosmotically-driven flow component through the channel that modulates at least one pressure-driven flow component resulting from the P1−P3 and the P2−P3 pressure differentials.

59. A method of controlling the flow of a fluid comprising: (a) placing a first accumulator at a first node, wherein the first node is in a first conduit having: a first fluid inlet in fluid communication with a first fluid source at pressure P1, a first fluid outlet at pressure P3, wherein P3<P1, and a first flow element disposed between the first fluid inlet and the first fluid outlet; (b) placing a second accumulator at a second node; wherein, the second node is in a second conduit having: a second fluid inlet in fluid communication with a second fluid source at pressure P2, wherein P3<P2, a second fluid outlet in fluid communication with the first conduit at the first node, a second flow element disposed between the second fluid inlet and the second fluid outlet, and a third fluid outlet at pressure P4, wherein P4<P1 and P4<P2, the third fluid outlet being in fluid communication at the second node with the second fluid inlet.

60. A method of controlling a flow of a fluid, comprising: applying an electric potential to spaced electrodes in electrical communication with a channel, the channel having a porous dielectric material disposed therein, the channel also having a fluid inlet in fluid communication at a node with a fluid source at pressure P1, the channel also having a fluid outlet in fluid communication with the fluid inlet and, at pressure P2, with a first fluid terminus, wherein P2<P1, and wherein a fluid storage element is disposed between the node and a second fluid terminus at pressure P3, wherein P3<P1, the fluid storage element having a fluid storage element inlet in fluid communication at the node with the fluid source, the fluid storage element also having a fluid storage element outlet in fluid communication with the fluid storage element inlet and the second fluid terminus, wherein the electric potential generates an electroosmotically-driven flow component through the channel that modulates at least one pressure-driven flow component resulting from the P1−P2 and the P1−P3 pressure differentials.

61. A method for controlling a flow of fluid, comprising: applying an electric potential to spaced electrodes in electrical communication with a channel, the channel having a porous dielectric material disposed therein, the channel also having a fluid inlet in fluid communication at a node with a fluid source at pressure P1, the channel also having a fluid outlet in fluid communication with the fluid inlet and, at pressure P2, with a first fluid terminus, wherein P2<P1, and wherein a fluid storage element is disposed between the node and the fluid inlet, the fluid storage element having a fluid storage element inlet in fluid communication at the node with the fluid source, the fluid storage element also having a fluid storage element outlet in fluid communication with the fluid storage element inlet and the fluid inlet, wherein the electric potential generates an electroosmotically driven flow component through the channel that modulates a pressure-driven flow component resulting from the P1−P2 pressure differential.