Claims
- 1. A method for generating mammalian neural crest stem cells comprising inducing neuroepithelial stem cells to differentiate in vitro, thereby generating said neural crest stem cells.
- 2. The method of claim 1 wherein said inducing comprises replating said neuroepithelial stem cells on a laminin-coated substrate.
- 3. The method of claim 2 wherein said inducing further comprises withdrawing a mitogen.
- 4. The method of claim 3 wherein said mitogen is FGF.
- 5. The method of claim 2 wherein said inducing further comprises withdrawing chick embryo extract.
- 6. The method of claim 1 wherein said inducing comprises withdrawing a mitogen.
- 7. The method of claim 6 wherein said mitogen is FGF.
- 8. The method of claim 1 wherein said inducing comprises withdrawing chick embryo extract.
- 9. The method of claim 1 wherein said inducing comprises adding a dorsalizing agent to the cells.
- 10. The method of claim 9 wherein said dorsalizing agent is a member selected from the group consisting of bone morphogenetic proteins.
- 11. The method of claim 10 wherein said dorsalizing agent is BMP-2.
- 12. The method of claim 10 wherein said dorsalizing agent is BMP-4.
- 13. The method of claim 10 wherein said dorsalizing agent is BMP-7.
- 14. A method of generating cells of the peripheral nervous system comprising the steps of:
(a) inducing neuroepithelial stem cells to differentiate in vitro, thereby generating neural crest stem cells; (b) inducing said neural crest stem cells to differentiate in vitro, thereby generating peripheral nervous system cells.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. application Ser. No. 08/852,744, filed May 7, 1997.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
[0002] This invention was made with government support under a FIRST award from the National Institutes of Health. The government has certain rights in the invention.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
08852744 |
May 1997 |
US |
Child |
09073881 |
May 1998 |
US |