Claims
- 1. An isolated nucleic acid comprising a nucleotide sequence that encodes an amino acid sequence having at least 85% identity with SEQ ID NO:2
- 2. An isolated nucleic acid comprising the nucleotide sequence having least 85% identity with SEQ ID NO:1
- 3. An isolated nucleic acid comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:2
- 4. An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:1
- 5. An isolated S16 ribosomal polypeptide comprising an amino acid sequence having least 85% identity to the sequence of SEQ ID NO:2
- 6. An isolated S16 ribosomal polypeptide comprising the amino acid sequence of SEQ ID NO:2
- 7. The isolated S16 ribosomal polypeptide of claim 6 which is labeled
- 8. The isolated S16 ribosomal polypeptide of claim 7 wherein the label is selected from the group consisting of: radiolabels, fluorescent labels, amino acid tags and biotin
- 9. The isolated S16 ribosomal polypeptide of claim 8 wherein said S16 ribosomal polypeptide comprises a radiolabel
- 10. The isolated S16 ribosomal polypeptide of claim 8 wherein said S16 ribosomal polypeptide comprises a fluorescent label.
- 11. The isolated S16 ribosomal polypeptide of claim 8 wherein said S16 ribosomal polypeptide comprises an amino acid tag.
- 12. The isolated S16 ribosomal polypeptide of claim 8 wherein said S16 ribosomal polypeptide comprises a biotin molecule
- 13. A vector comprising the nucleic acid of claim 1
- 14. A host cell comprising the vector of claim 13
- 15. A method of making isolated an S16 ribosomal polypeptide comprising:
a) introducing the nucleic acid of claim 1 into a host cell b) maintaining said host cell under conditions whereby said nucleic acid is expressed to produce said S16 ribosomal polypeptide c) purifying said S16 ribosomal polypeptide
- 16. A method for testing for inhibitors of ribosomal assembly comprising the steps of:
a.) contacting at least one direct binding ribosomal polypeptide selected from the group consisting of S4, S7, S8, S15, S17 and S20 with 16S ribosomal RNA to form a polyribonucleotide protein complex and; b) contacting said polyribonucleotide protein complex with S16 ribosomal polypeptide
(i) in the presence of a test agent; and (ii) in the absence of said test agent; and c) determining the amount of S16 ribosomal polypeptide bound to the polyribonucleotide protein complex
(i) in the presence of a test agent; and (ii) in the absence of said test agent; and d) comparing the amount of S16 ribosomal polypeptide determined in step (c)(i) to the amount of S16 ribosomal polypeptide determined in step (c)(ii);
- 17. The method of claim 16 wherein the direct binding ribosomal proteins comprise S4, S7, S8.
- 18. The method of claim 16 wherein the direct binding ribosomal proteins comprise S4, S7, S8 and S17.
- 19. The method of claim 16 wherein the direct binding ribosomal proteins comprise S4, S7, S8, S17, S15.
- 20. The method of claim 16 wherein the direct binding ribosomal proteins comprise S4, S7, S8, S17, S15 and S20
- 21. The method of claim 16 wherein the S16 ribosomal polypeptide is labelled
- 22. The method of claim 16 wherein S16 ribosomal polypeptide comprises a radiolabel
- 23. The method of claim 16 wherein S16 ribosomal polypeptide comprises an amino acid tag.
- 24. The method of claim 16 wherein wherein S16 ribosomal polypeptide comprises comprises a biotin molecule
- 25. The method of claim 16 wherein said 16S ribosomal RNA is labeled
- 26. The method of claim 16 wherein said 16S ribosomal RNA comprises a radiolabel
- 27. The method of claim 16 wherein said 16S ribosomal RNA comprises a biotin molecule
- 28. The method of claim 16 wherein wherein S16 ribosomal polypeptide is attached to a solid support.
- 29. The method of claim 16 wherein said 16S ribosomal RNA is attached to a solid support
- 30. A method for testing for inhibitors of ribosomal assembly comprising the steps of:
a.) contacting at least one direct binding ribosomal polypeptide selected from the group consisting of S4, S7, S8, S15, S17 and S20 with 16S ribosomal RNA to form a polyribonucleotide protein complex and; b) contacting said polyribonucleotide protein complex with at least one non-direct binding ribosomal polypeptide selected from the group consisting of S1, S2, S3, S5, S6, S9, S10, S11, S12, S13, S14, S16, S18, S19, and S21.
(iii) in the presence of a test agent; and (iv) in the absence of said test agent; and c) determining the amount of at least one non-direct binding ribosomal polypeptide bound to the RNA
(i) in the presence of a test agent; and (ii) in the absence of said test agent; and d) comparing the amount of least one non direct binding ribosomal polypeptide in step (c)(i) to the amount of non-direct binding ribosomal polypeptide protein determined in step (c)(ii);
- 31. The method of claim 30 wherein the direct binding ribosomal proteins comprise S4, S7, S8.
- 32. The method of claim 30 wherein the direct binding ribosomal proteins comprise S4, S7, S8 and S17.
- 33. The method of claim 30 wherein the direct binding ribosomal proteins comprise S4, S7, S8, S17, S15.
- 34. The method of claim 30 wherein the direct binding ribosomal proteins comprise S4, S7, S8, S17, S15 and S20
- 35. The method of claim 30 wherein the non-direct binding ribosomal proteins comprise S16
- 36. The method of claim 30 wherein the non-direct binding ribosomal proteins comprise S3, S5, S9, S10, S12, S14, S16 and S19
- 37. The method of claim 30 wherein said direct binding or non-direct binding ribosomal polypeptide is labeled
- 38. The method of claim 30 wherein said direct binding or non-direct binding ribosomal polypeptide comprises a radiolabel
- 39. The method of claim 30 wherein said direct binding or non-direct binding ribosomal polypeptide comprises an amino acid tag.
- 40. The method of claim 30 wherein said said direct binding or non-direct binding ribosomal polypeptide comprises a biotin molecule
- 41. The method of claim 30 wherein said 16S ribosomal RNA is labeled
- 42. The method of claim 30 wherein said 16S ribosomal RNA comprises a radiolabel
- 43. The method of claim 30 wherein said 16S ribosomal RNA comprises a biotin molecule
- 44. The method of claim 30 wherein said direct binding or non-direct binding ribosomal polypeptide is attached to a solid support.
- 45. The method of claim 30 wherein said 16S ribosomal RNA is attached to a solid support
- 46. A method for testing for inhibitors of ribosomal assembly comprising the steps of:
a.) contacting S4, S7, S8, S17 and S20 ribosomal polypeptides with 16S ribosomal RNA to form apolyribonucleotide protein complex and; b) contacting said polyribonucleotide protein complex with non-direct binding ribosomal polypeptides S3, S5, S9, S10, S12, S14, S16 and S19 to form a resultant polyribonucleotide protein complex
(v) in the presence of a test agent; and (vi) in the absence of said test agent; and b) contacting non-direct binding ribosomal polypeptide S3 with said resultant polyribonucleotide protein complex; and c) determining the amount of said non-direct binding ribosomal polypeptide S3 bound to said resultant polyribonucleotide protein complex;
(i) formed in the presence of said test agent; and (ii) formed in the absence of said test agent; and e) comparing the amount of S3 determined in step (c)(i) to the amount of S3 determined in step (c)(ii)
- 47. The method of claim 46 wherein said non-direct binding ribosomal polypeptide S3 is labeled.
- 48. The method of claim 47 wherein said non-direct binding ribosomal polypeptide S3 is radiolabeled
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of the following provisional application: Application Serial No. 60/219360 filed Jul. 19, 2000 under 35 U.S.C 119(e)(1).
Provisional Applications (1)
|
Number |
Date |
Country |
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60219360 |
Jul 2000 |
US |