COMPOSITE BODY OF INORGANIC COMPOUND AND CELLS AND PRODUCTION METHOD THEREOF

Abstract

A composite body of an inorganic compound and cells includes animal cells filled with fine particles of the inorganic compound, and the fine particles of the inorganic compound adhered to an outer portion of the animal cells. A method of producing a composite body of an inorganic compound and cells is also provided, including cultivating animal cells in a culture medium containing fine particles of the inorganic compound, and separating a solid component from the cultivated product.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a transmission electron microscopic (TEM) photograph (×400) of the composite body of hydroxyapatite particles and cells, MDCK-HA, produced in Example 1;

FIG. 2 is a TEM photograph (×1,800) of MDCK-HA;

FIG. 3 is a scanning electron microscopic (SEM) photograph (×4,500) of MDCK-HA showing the HA particles as is attached to and is impregnated into the MDCK cells;

FIG. 4 is a SEM photograph (×3,500) of MDCK-HA showing the MDCK cells in the process of importing the HA particles;

FIG. 5 is a TEM photograph (×7,000) of the HA nanoparticles as a single body;

FIG. 6 is a TEM photograph (×400) of the MDCK cell as a single body;

FIG. 7 is a TEM photograph (×1,200) of the composite body (HOS-HA) produced in Example 2;

FIG. 8 is a TEM photograph (×1,500) of the dried product of the composite body (HOS-HA) produced in Example 2;

FIG. 9 is a TEM photograph (×3,000) of the composite body of silica particles and cells produced in Example 3;

FIG. 10 is a TEM photograph (×7,000) of the silica particles as a single body;

FIG. 11 is a TEM photograph (×700) of the composite body of alumina particles and cells produced in Example 4; and

FIG. 12 is a TEM photograph (×7,000) of the alumina particles as a single body.

Claims

1. A composite body of an inorganic compound and cells comprising: animal cells filled with fine particles of said inorganic compound; andsaid fine particles of said inorganic compound adhered to an outer portion of the animal cells.

2. The composite body according to claim 1, wherein said fine particles of said inorganic compound have an average particle size of 10 nm to 10 μm.

3. The composite body according to claim 1, wherein said fine particles of said inorganic compound are coated with a polymeric nitrogen compound.

4. The composite body according to claim 3, wherein said polymeric nitrogen compound has a number average molecular weight of 800 to 100,000.

5. The composite body according to claim 3, wherein said polymeric nitrogen compound comprises a primary amino group, a secondary amino group and a tertiary amino group in a repeating structure unit thereof.

6. The composite body according to claim 3, wherein said polymeric nitrogen compound is a straight chain, branched chain or cyclic polyamine compound or a mixture thereof.

7. The composite body according to claim 6, wherein said polyamine compound is polyethylene imine.

8. The composite body according to claim 1, wherein said fine particles of said inorganic compound comprises one of a calcium phosphate compound and metal oxide.

9. The composite body according to claim 8, wherein said calcium phosphate compound comprises hydroxyapatite.

10. The composite body according to claim 8, wherein said metal oxide comprises aluminum oxide, silica, magnesium oxide or ferric oxide.

11. A method of producing a composite body of an inorganic compound and cells comprising: cultivating animal cells in a culture medium containing fine particles of the inorganic compound; andseparating a solid component from the cultivated product.

12. The production method according to claim 11, wherein said inorganic compound is contained in a concentration of 1.0 pg/cell to 1.0 ng/cell with respect to the animal cells.

13. The production method according to claim 11, wherein said cultured animal cells are deactivated after separation of said solid component from said cultivated product.

14. The production method according to claim 13, wherein said deactivation treatment comprises one of a heating treatment and a chemical treatment.

15. The production method according to claim 14, wherein said chemical treatment comprises an immobilization of a protein with an aldehyde compound or a dimaleimide compound.

16. The production method according to claim 11, wherein said fine particles of the inorganic compound are coated with a polymeric nitrogen compound.

17. The production method according to claim 16, wherein said polymeric nitrogen compound has a number average molecular weight of 800 to 100,000.

18. The production method according to claim 16, wherein said polymeric nitrogen compound comprises a primary amino group, a secondary amino group and a tertiary amino group in a repeating structure unit thereof.

19. The production method according to claim 16, wherein said polymeric nitrogen compound comprises one of a straight chain, a branched chain and cyclic polyamine compound, or a mixture thereof.

20. The production method according to claim 19, wherein said polyamine compound comprises polyethyleneimine.

21. The production method according to claim 11, wherein said fine particles of said inorganic compound comprise one of a calcium phosphate compound and metal oxide.

22. The production method according to claim 21, wherein said calcium phosphate compound comprises hydroxyapatite.

23. The production method according to claim 21, wherein said metal oxide comprises one of aluminum oxide, silica, magnesium oxide and ferric oxide.